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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </location>
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        </location>
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            <end position="240"/>
        </location>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYF5</accession>
    <name>A0AAU9EYF5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
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        </recommendedName>
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        <name evidence="17" type="ORF">DMAD_08577</name>
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    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EYF5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Casein kinase I gamma"/>
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    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Casein_kinase-1_gamma_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12605">
        <property type="entry name" value="CK1gamma_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="62"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="380"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="395"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
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</entry>
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            <authorList>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EYH5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000090">
        <property type="entry name" value="two pore potassium channel protein sup-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003092">
        <property type="entry name" value="2pore_dom_K_chnl_TASK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF326">
        <property type="entry name" value="ACID-SENSITIVE TWO PORE DOMAIN K+ CHANNEL DTASK-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038061">
        <property type="entry name" value="K_channel_subfamily_K_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01095">
        <property type="entry name" value="TASKCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13 16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 12 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="6 12" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4 12" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="15">
        <location>
            <begin position="68"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="15">
        <location>
            <begin position="169"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR038061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF90795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="407" mass="45392" checksum="6BB23CDD6E3B14B3" modified="2024-11-27" version="1">MKKQNVRTISLIVCTFTYLLVGAAVFDALESETEKRRWEALQDAEDMIIRKYSISPEDFKVMETVVLKSESHKAGQQWKFTGAFYYATTVLTTIGYGHSTPSTVGGKLFTMCYAIVGIPLGLVMFQSIGERVNRLSSYVIQAVRTSLRCKRTVASEVDLICVVTTLSSLTIAGGAAAFSKFEGWSYFDSVYYCFITLTTIGFGDMVALQRDNALNRKPEYVMFALIFILFGLAIVAASLNLLVLRFVTMNTEDERRDEAQAMQALQVAVKLEGDVITSNGSILSGYEGHDGQSLNGSSASSMCSCHCVCLNRNRHKKSNHLGRNNDTENQYRLQRSPTHIRHLLPEVVPMQDLNYDYDTQSVHSMPDRGIMDSNYMGVDMVDTASSGSPLRPQQLLKRNVSLLSFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYI9</accession>
    <name>A0AAU9EYI9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transcription factor Ouib</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_08227</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EYI9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF5">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 44 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="13">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="271"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF89474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="309" mass="35930" checksum="4A94669F8E1F6411" modified="2024-11-27" version="1">MIAGIRLRDLTNVPGFICHCCQSDLKMAMTFRKLCLKTQKRWQPKIVKDEDSSLSEMECHAQAEPLKGKIKNCSKVSTFMETDDEPKEPEEPFIIMLEEQPTSNIFECEQIDEEEANNLDLDIVIKCEPEGSSSEAEQLTSDPILTKSSHKVKSEDEPYICELCGTHSHSKTVFERHMRKHTGERPYGCLECTAKFFTAAELRSHHRVHTGERPFACRYCERRYVSYMGRLKHERIHTNERPFVCAECGKAFTNSYILKNHMLVHTGERQFRCDLCERSFQRKTHLRTHFQSNTHQQNLVKQQQLDGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYJ7</accession>
    <name>A0AAU9EYJ7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Golgi-associated plant pathogenesis-related protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_09678</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EYJ7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
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    <sequence length="184" mass="20629" checksum="2FFA02B16669DB9A" modified="2024-11-27" version="1">MQAKIILIFLAIYIAPIHGDFAQDALKRHNEIRKGHGCPDLQLDDELSTDCQAYAKTLSEKGKIEPSNGDGYTENLCITILKPIDCLESWYMERALHDYSAPRLNAETEHFTAMIWKSSTKLGIGLVTKDDNNYVVARYKPKGNVKSEFKENVPRPTGKADHATSFSFGVLVLGSVFIQYFSDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYK4</accession>
    <name>A0AAU9EYK4_DROMD</name>
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        <recommendedName>
            <fullName evidence="12">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="12">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
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        <name evidence="16" type="ORF">DMAD_08621</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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    </comment>
    <comment type="pathway">
        <text evidence="3 12">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 11 12" id="KW-0833">Ubl conjugation pathway</keyword>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU369009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF90009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYK5</accession>
    <name>A0AAU9EYK5_DROMD</name>
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        <recommendedName>
            <fullName evidence="4">PID domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">DMAD_09276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EYK5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="entry name" value="FI19613P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21219:SF4">
        <property type="entry name" value="PID DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="609"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="653"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="690"/>
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        </location>
    </feature>
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            <begin position="869"/>
            <end position="893"/>
        </location>
    </feature>
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        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
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        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
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        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="403"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="610"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="654"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="690"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF90825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1054" mass="112900" checksum="E60654E5C4C1F124" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYL2</accession>
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        <submittedName>
            <fullName evidence="5">Arrestin domain-containing protein 17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_08248</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EYL2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF167">
        <property type="entry name" value="ARRESTIN C-TERMINAL-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
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        <location>
            <begin position="173"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="318"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="318"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="341"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF89504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="531" mass="60120" checksum="E2FB17C71F396E17" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYM1</accession>
    <name>A0AAU9EYM1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Gamma-aminobutyric acid type B receptor subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">G-protein coupled receptor 51</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_09701</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EYM1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004965">
        <property type="term" value="F:G protein-coupled GABA receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15294">
        <property type="entry name" value="7tmC_GABA-B-R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06366">
        <property type="entry name" value="PBP1_GABAb_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000063">
        <property type="entry name" value="Gamma-aminobutyric acid type B receptor subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000072">
        <property type="entry name" value="Gamma-aminobutyric acid type B receptor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002455">
        <property type="entry name" value="GPCR3_GABA-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519">
        <property type="entry name" value="GABA-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519:SF74">
        <property type="entry name" value="GAMMA-AMINOBUTYRIC ACID TYPE B RECEPTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01177">
        <property type="entry name" value="GABAB1RECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01176">
        <property type="entry name" value="GABABRECEPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12 22" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="506"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="581"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="639"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="680"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="703"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="21">
        <location>
            <begin position="465"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="935"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1071"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="953"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="979"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1006"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1100"/>
            <end position="1110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="BFF91412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1242" mass="140824" checksum="4E63D580952D53BB" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYM6</accession>
    <name>A0AAU9EYM6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Cadherin-86C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_09900</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000246">
        <property type="entry name" value="Cadherin 86C, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000295">
        <property type="entry name" value="Cadherin 86C, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000098">
        <property type="entry name" value="cadherin-23 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000289">
        <property type="entry name" value="cadherin-86C isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000259">
        <property type="entry name" value="cadherin-86C isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026:SF96">
        <property type="entry name" value="CADHERIN-86C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026">
        <property type="entry name" value="FAT ATYPICAL CADHERIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 13" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="222"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="324"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="441"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="558"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="666"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="732"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
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            <begin position="971"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1339"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1383"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1413"/>
            <end position="1465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1494"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1581"/>
            <end position="1613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1627"/>
            <end position="1651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1663"/>
            <end position="1872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="985"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1383"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1441"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1502"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1585"/>
            <end position="1597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1627"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1667"/>
            <end position="1679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1707"/>
            <end position="1722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1758"/>
            <end position="1768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1819"/>
            <end position="1850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00059331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF91675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1904" mass="215339" checksum="B747BCFA82045936" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYM9</accession>
    <name>A0AAU9EYM9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CLIP domain-containing serine protease</fullName>
            <ecNumber evidence="11">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_09301</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The clip domain consists of 35-55 residues which are 'knitted' together usually by 3 conserved disulfide bonds forming a clip-like compact structure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10 12">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="11"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EYM9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000028">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000084">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.30:FF:000005">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022700">
        <property type="entry name" value="CLIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038565">
        <property type="entry name" value="CLIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12032">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00680">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51888">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 11 15" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043101557" description="CLIP domain-containing serine protease" evidence="12">
        <location>
            <begin position="23"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Clip" evidence="14">
        <location>
            <begin position="38"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="13">
        <location>
            <begin position="131"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF90855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="395" mass="43541" checksum="03AF8A320D2EA48A" modified="2024-11-27" version="1">MFNKEYICLIFGILASLSAASGQFYFPNEAAQVPNYGRCITPNRERALCIHLEDCKYLYGVLTTSPLRDTDRLYLSRSQCGYTNGKVLICCLDRYRDTSPNTTPQTVQPNITNSNLLPLPGQCGNLLSNRIYGGTRTKIDEFPWMALIEYTKTAGKKGHHCGGSLISTRYVVTASHCVNGKALPTDWRLTGVRLGEWDTATDPDCEVDVRGMKDCAPAHLDVPVERAIPHPEYIPSSKNQVNDIALLRLGRQVEYTDFVRPICLPLDVNLRSATFDGITMDVAGWGKTEELSSSNMKLKAAVEGTRMDDCQAVYNRQDIMLEDTQMCAGGKEGIDSCRGDSGGPLIGLDTNKVNTYYFLAGVVSFGPTPCGLAGWPGVYTLVGKYVDWIQNTIEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F1T8</accession>
    <name>A0AAU9F1T8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Transcription factor kayak</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_09665</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Homodimer. Heterodimer with Jra. The kay-Jra heterodimer binds more stably to the AP-1 site than either of the two proteins alone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. Fos subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F1T8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000006">
        <property type="entry name" value="fos-related antigen 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000837">
        <property type="entry name" value="AP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351:SF24">
        <property type="entry name" value="ACTIVATING TRANSCRIPTION FACTOR 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351">
        <property type="entry name" value="FOS TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00042">
        <property type="entry name" value="LEUZIPPRFOS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="11">
        <location>
            <begin position="277"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="164"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="370"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="566"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="243"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="399"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF91364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="594" mass="62304" checksum="7648E20489EC740A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F1U5</accession>
    <name>A0AAU9F1U5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Synapse-associated protein of 47 kDa</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_08415</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F1U5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048172">
        <property type="term" value="P:regulation of short-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3970.10:FF:000001">
        <property type="entry name" value="synapse-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051494">
        <property type="entry name" value="BSD_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019:SF6">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD" evidence="3">
        <location>
            <begin position="285"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="163"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="473"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="505"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HOMEOBOX"/>
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        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="167"/>
            <end position="227"/>
        </location>
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        <location>
            <begin position="169"/>
            <end position="228"/>
        </location>
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        <location>
            <begin position="219"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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            <dbReference type="ARBA" id="ARBA00023155"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF91644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="275" mass="30982" checksum="451F4A876F0D98D3" modified="2024-11-27" version="1">MSIQLAPLHIPAIRPGPGFETDASVAVKRHAAPWPYNDDGFNQYHASASQYYMEQRDRKPMFPYYPDTQFQPYWANYRDQPTSAAAAAYMSATDDRHVTVSAAAAARQSVEGTSQSSYETPTYSSPGGLRGYPGEAYSSTGASGGLSVGAVGPCTPTNPLHEWTGAVSVRKKRKPYSKFQTLELEKEFLFNAYVSKQKRWELARNLQLTERQVKIWFQNRRMKNKKNSQRQANQQNNNNNSSSNHNHSQTAQQHHNNHHLNLGLSMGHHATKMHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F1X7</accession>
    <name>A0AAU9F1X7_DROMD</name>
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        <submittedName>
            <fullName evidence="4">Peroxidase</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ORF">DMAD_09886</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Neoptera</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="OXIDASE/PEROXIDASE"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1 4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043975665" evidence="3">
        <location>
            <begin position="22"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="2">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
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        </ligandPart>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR619791-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF91659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="695" mass="77048" checksum="3CCDF620AB771B55" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F1Y6</accession>
    <name>A0AAU9F1Y6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_08468</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F1Y6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000596">
        <property type="entry name" value="CK1 family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="12"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF89794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="340" mass="40038" checksum="639452065FFBF3DC" modified="2024-11-27" version="1">MDPLEFTVAEKYRLIERIGNGSFGELYRAIDIGNGEEVAAKVELVKAAQPMLEREAKIYKILAGGKGIPQVRYYGIERDVNVLVLDLLGPTLEDLLNFCMRRFSLNTILVLAGQMLARLEFVHQKCFVHRDIKPENFLMGQGAQRNVVYLIDFGLAKNFYNPSTAKHIEYRQNRELVGTPRYASVRAHFAEQGRRDDLESLGYILIYFWHGRLPWQSIQSNSRAQKLEQMAEIKANMPLDNLCAGMPDEFLMYLRYVRCLHFDEMPDYMYLRQLFRNLFCRRRWTNDFFFDWVDKQSSVQHQRDCVAKRDKQQILKKNLNCCSCSYHKRRPRMDHQRGQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F1Y9</accession>
    <name>A0AAU9F1Y9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Neprilysin-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_09741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F1Y9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018497">
        <property type="entry name" value="Peptidase_M13_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF240">
        <property type="entry name" value="GH14155P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01431">
        <property type="entry name" value="Peptidase_M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M13 C-terminal" evidence="1">
        <location>
            <begin position="25"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF91469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="235" mass="27060" checksum="0DAFFCEC798C02AF" modified="2024-11-27" version="1">MGRLFEEELPAPSDPVEAYEARVRYRPVQRQIDIGLGLLQAPFYDPHYGQSLRYATLGVAVARQMAMAFDDPHWSVDLLERDHWDGLTAWRYHDRSECFSRQVEEYLHANASATRQLIADSAALNVAFRAYLTWLGFKEPNNDFNLLSKETLPGLNYSNTALFFVAYAQQHCSREESHRWSSSWAKELPSYSAQQNHTWPRLQVNGPLRNLEDFAKEFHCPIGSAMNPATKCNIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F272</accession>
    <name>A0AAU9F272_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst complex component Sec8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_09854</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the SEC8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032584">
        <property type="term" value="C:growth cone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039682">
        <property type="entry name" value="Sec8/EXOC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007191">
        <property type="entry name" value="Sec8_exocyst_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04048">
        <property type="entry name" value="Sec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec8 N-terminal" evidence="8">
        <location>
            <begin position="425"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="L27" evidence="7">
        <location>
            <begin position="526"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="805"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1040"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="808"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF91619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1359" mass="152435" checksum="FF4827B16CD9DD39" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F284</accession>
    <name>A0AAU9F284_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">C-terminal-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_09173</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F284"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001221">
        <property type="term" value="F:transcription coregulator binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05299">
        <property type="entry name" value="CtBP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000012">
        <property type="entry name" value="C-terminal-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043322">
        <property type="entry name" value="CtBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051638">
        <property type="entry name" value="CTBP_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029:SF7">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="7">
        <location>
            <begin position="38"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="8">
        <location>
            <begin position="134"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="381"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="450"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="384"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="479"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF90696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="489" mass="52106" checksum="1D64E2F6874C34B1" modified="2024-11-27" version="1">MDKNLMMPKRSRIDVKGSFANGPLQARPLVALLDGRDCSIEMPILKDVATVAFCDAQSTSEIHEKVLNEAVGALMWHTIILTKEDLEKFKALRIIVRIGSGTDNIDVKAAGELGIAVCNVPGYGVEEVADTTMCLILNLYRRTYWLANMVREGKKFTGPEQVREAAHGCARIRGDTLGLVGLGRIGSAVALRAKAFGFNVIFYDPYLPDGIDKSLGLTRVYTLQDLLFQSDCVSLHCTLNEHNHHLINEFTIKQMRPGAFLVNTARGGLVDDETLALALKQGRIRAAALDVHENEPYNVFQGALKDAPNLICTPHAAFFSDASATELREMAATEIRRAIVGNIPDVLRNCVNKEYFMRTPPTAAAGGVAAAVYPEGALHHRAHSTTPHDGPHSTTNIGSSSSSSALAQPPPPPNSVAAAAAAVAAAAAALLPSPVPPTVVPTVPHLSPQVGGLPLGIVSSQSPLSAPDPNNHLLSSIKAEVKAESTETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F287</accession>
    <name>A0AAU9F287_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein CLEC16A homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_08595</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CLEC16A/gop-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F287"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901096">
        <property type="term" value="P:regulation of autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039272">
        <property type="entry name" value="CLEC16A/TT9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045820">
        <property type="entry name" value="CLEC16A/TT9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019155">
        <property type="entry name" value="CLEC16A/TT9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21481">
        <property type="entry name" value="PROTEIN CLEC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21481:SF0">
        <property type="entry name" value="PROTEIN CLEC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19439">
        <property type="entry name" value="CLEC16A_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09758">
        <property type="entry name" value="FPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FPL" evidence="4">
        <location>
            <begin position="50"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="CLEC16A/TT9 C-terminal" evidence="5">
        <location>
            <begin position="247"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="CLEC16A/TT9 C-terminal" evidence="5">
        <location>
            <begin position="439"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="829"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="872"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1030"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="837"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="872"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="912"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="922"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="946"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="970"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1032"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1053"/>
            <end position="1064"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF89974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1064" mass="119011" checksum="1B4BEFEF9FC3F158" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F294</accession>
    <name>A0AAU9F294_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Metastasis-associated protein MTA3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_09186</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Plays a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels. Contributes to transcriptional repression by BCL6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the nucleosome remodeling and deacetylase (NuRD) repressor complex, composed of core proteins MTA1, MTA2, MTA3, RBBP4, RBBP7, HDAC1, HDAC2, MBD2, MBD3, and peripherally associated proteins CDK2AP1, CDK2AP2, GATAD2A, GATAD2B, CHD3, CHD4 and CHD5. The exact stoichiometry of the NuRD complex is unknown, and some subunits such as MBD2 and MBD3, GATAD2A and GATAD2B, and CHD3, CHD4 and CHD5 define mutually exclusive NuRD complexes. Interacts with BCL6. Interacts with NACC2. Interacts with PWWP2B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the metastasis-associated protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F294"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016581">
        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04709">
        <property type="entry name" value="BAH_MTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11661">
        <property type="entry name" value="SANT_MTA3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000022">
        <property type="entry name" value="Blast:Metastasis-associated protein MTA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000012">
        <property type="entry name" value="Metastasis-associated 1 family, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.50:FF:000001">
        <property type="entry name" value="Metastasis-associated 1 family, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000012">
        <property type="entry name" value="metastasis-associated protein MTA3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000949">
        <property type="entry name" value="ELM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040138">
        <property type="entry name" value="MIER/MTA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035170">
        <property type="entry name" value="MTA1_R1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10865:SF29">
        <property type="entry name" value="METASTASIS ASSOCIATED 1-LIKE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10865">
        <property type="entry name" value="METASTASIS-ASSOCIATED PROTEIN AND MESODERM INDUCTION EARLY RESPONSE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01448">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17226">
        <property type="entry name" value="MTA_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01189">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51156">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="19">
        <location>
            <begin position="4"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="ELM2" evidence="20">
        <location>
            <begin position="204"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="21">
        <location>
            <begin position="322"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="530"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="63"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="729"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="84"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00058654"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00065659"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00071581"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00093454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="BFF90711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="858" mass="94251" checksum="D354A11BCC1061FB" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBV0</accession>
    <name>A0AAU9FBV0_DROMD</name>
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        <recommendedName>
            <fullName evidence="2">Superoxide dismutase copper/zinc binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">DMAD_11034</name>
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    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
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            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BFF93130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBV0"/>
    <dbReference type="Proteomes" id="UP001500889">
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Cu-only_SOD"/>
    </dbReference>
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        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SUPEROXIDE DISMUTASE COPPER_ZINC BINDING DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043806984" description="Superoxide dismutase copper/zinc binding domain-containing protein" evidence="1">
        <location>
            <begin position="33"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="2">
        <location>
            <begin position="701"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF93130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1137" mass="127969" checksum="8117E74DEA7A4A0B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBV1</accession>
    <name>A0AAU9FBV1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fibroleukin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_11098</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
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        <location>
            <begin position="23"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF93206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="190" mass="22065" checksum="A991D53CBB16CD3C" modified="2024-11-27" version="1">MAMNAWNCFVYIMLGVIFLASANSVDSLADIGVDQESCCSSSDLEDQCGFVCYPILKPLLQYLEKCQEMDLQIKELEDMFREQSVEIIKYKSKTEVLSKHLENNFCDTKVMELGDAIDVRIDVQEEIKKLKLELASKESDIIDMNSKLFKKYELLEQKNKSLKETETAWVITIRRGMYHGIWLDHRSERT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBV5</accession>
    <name>A0AAU9FBV5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_11064</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the TIM23 complex at least composed of Tim23, Tim17 (Tim17a1, Tim17a2 or Tim17b1) and a Tim50.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Mitochondrion inner membrane</location>
            <topology evidence="2 14">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the TIM50 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBV5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000019">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10 14" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="FCP1 homology" evidence="15">
        <location>
            <begin position="129"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF93167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="336" mass="39339" checksum="A2C6D5BDFEC03738" modified="2024-11-27" version="1">MRTSNISRSCSDSSSGKNITHKKVTEENSLWKLKYTSSSNASKDLYNFGRISVCSLVVGGFCSVVLWAIYELGKPEVDQHGLSIEDEFSGLPWAREYLERMWHSLQYYQKMLEEPLPEKLLPDTLPPPYIQPPYTLVLEIKDILVHLDWTYQTGWRFKKRPGVDFFLRKCSKHFEIVVYTSEQGMTAFPLLDALDPYGYICYRVVRGDTELVEGQHVKNLNNLNRNLSRVVVVDWDRNATPQHPNNTFVMTRWLGNDDDVQLFDLTAFLELLAEHHVNDVREILKYYSRFDDPIEQFRENQRRLLEIEEKTNRKAMQIQRNWTASLLGQYARGTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBV6</accession>
    <name>A0AAU9FBV6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_11031</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
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</entry>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    </evidence>
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        <source>
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</entry>
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</entry>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </source>
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        </location>
    </feature>
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        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
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        </source>
    </evidence>
    <sequence length="1394" mass="154231" checksum="7B536314A1EDB679" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="463"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="501"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="541"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="582"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="641"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="682"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="707"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="472"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="819"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="752"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="824"/>
            <end position="837"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF93250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="847" mass="95207" checksum="D6AD810FAC49066A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FC77</accession>
    <name>A0AAU9FC77_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Trypsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_11158</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FC77"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000077">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
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    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF94">
        <property type="entry name" value="AT20289P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 11" id="KW-0720">Serine protease</keyword>
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    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
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    <feature type="chain" id="PRO_5043639202" evidence="12">
        <location>
            <begin position="22"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="13">
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            <begin position="32"/>
            <end position="257"/>
        </location>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF93278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="259" mass="27186" checksum="4883AF2749AE29E4" modified="2024-11-27" version="1">MNRLLLCVVAVVALGAGCVQAHPDIDFPFGRIVNGEDTSIESHPWQVSIQTSAKGSHFCGGSLINQDTVVTAAHCMQSYAASEMQVRLGSTSRSSGGEVVSVKAFKYHEGYNSKLMVNDVAVIKLSSPVRETAKVRYVTLASSTPSSGTPAVVTGWGTTCFLLCSSPDSLMAVGVDILQKQDCASDSYSYGESILDTMVCASGEKKDACQGDSGGPLVANGELVGVVSWGNGCAWTGYPGVYADVAPLRSWILKTAEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FC81</accession>
    <name>A0AAU9FC81_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Proton-coupled amino acid transporter-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_11190</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FC81"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF349">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="458"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="76"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF93318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="485" mass="53227" checksum="ACA14C65346E92C9" modified="2024-11-27" version="1">MTERQPLLLQSDASDYEGSRGGPARPARSSPPDNTLVNVHSEDSIAASGSGSGDDDQAALDKASYNPTLHRTLEHPTSNSETLVHLLKGNIGTGILAMPDAFKNAGLYVGLFGTLIMGAICTHCMHMLVNCSHELCRRLQQPALDFSDVAYCSFETGPLGLRRYSLLARRIVTTFLFITQIGFCCVYFLFVALNIKDVMDHYFKIDVHIYLLIMLMPMIVLNLVRNLKYLTPVSLIAAILTVAGLAITFSYMLHDLPDVHTVKPIASWATLPLYFGTAIYAFEGIGVVLPLENNMRTPDDFGGTTGVLNTGMVVVACLYTSVGFFGYLKYGEDVKGSITLNLPQGDGLSQLVRLTMAVAIFLSYTLQFYVPVNIVEPFVRSHFDTTRAKDLAATILRTVLVTFTFLLATCIPNLGSIISLVGAVSSSALALIAPPIIEIITYYHVGYGRYNWMLWKDFLILIFGLCGFVFGTWASLAQILNDRTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FC84</accession>
    <name>A0AAU9FC84_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">39S ribosomal protein L59, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_11163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
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            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="40279" checksum="352709D812BD31C9" modified="2024-11-27" version="1">MAELKTFSLLANVCKCFLVRGNAFSCWSRALSKYAISPLNMNSDTLQESQGNAGWSKNGPLSTLLRNVSESMKLIERTGYECSKVATQQKVNGNILQRHLPQKQNEQQVSARSYASPRPRKQTLFGCNDSNLVSKYARIWESRCEGYASQSKPNWRDRKMEPPVEGEKPTSYRQEYVWNIKKTPPKEYTTLPESRWIGEQQSLKEQQRLKEQLADYIDIASITNKIKRNDRIMRCIRGVEHNAHLPVDFKPQVYRHGYHWNLKQSEIIAKYKDDKIPEMIKMQQQQKRKKEMSERKDLRKVTKKQQLPHRTVSEVKIINKAKKVKKSIMESSSKAKLSKRYPRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FC85</accession>
    <name>A0AAU9FC85_DROMD</name>
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        <submittedName>
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        </submittedName>
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        <name evidence="5" type="ORF">DMAD_11169</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5 6">RF1</strain>
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        <text evidence="3">Belongs to the choline/ethanolamine kinase family.</text>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RE24176P"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="2" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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            <end position="155"/>
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            <begin position="351"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="528" mass="60029" checksum="9582C807362B4B58" modified="2024-11-27" version="1"> </sequence>
</entry>
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    <accession>A0AAU9FC87</accession>
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        <submittedName>
            <fullName evidence="8">Proton-coupled amino acid transporter-like protein</fullName>
        </submittedName>
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        <name evidence="8" type="ORF">DMAD_11190</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FC87"/>
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        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
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        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF349">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="228"/>
        </location>
    </feature>
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            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="374"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="479"/>
        </location>
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        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="489" mass="53605" checksum="C8547B7E011B91B9" modified="2024-11-27" version="1">MTERQPLLLQSDASDYEGSRGGPARPARSSPPDNTLVNVHSEDSIAVHAAASGSGSGDDDQAALDKASYNPTLHRTLEHPTSNSETLVHLLKGNIGTGILAMPDAFKNAGLYVGLFGTLIMGAICTHCMHMLVNCSHELCRRLQQPALDFSDVAYCSFETGPLGLRRYSLLARRIVTTFLFITQIGFCCVYFLFVALNIKDVMDHYFKIDVHIYLLIMLMPMIVLNLVRNLKYLTPVSLIAAILTVAGLAITFSYMLHDLPDVHTVKPIASWATLPLYFGTAIYAFEGIGVVLPLENNMRTPDDFGGTTGVLNTGMVVVACLYTSVGFFGYLKYGEDVKGSITLNLPQGDGLSQLVRLTMAVAIFLSYTLQFYVPVNIVEPFVRSHFDTTRAKDLAATILRTVLVTFTFLLATCIPNLGSIISLVGAVSSSALALIAPPIIEIITYYHVGYGRYNWMLWKDFLILIFGLCGFVFGTWASLAQILNDRTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FC88</accession>
    <name>A0AAU9FC88_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_11146</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF51">
        <property type="entry name" value="ANOCTAMIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="421"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="529"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="670"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="723"/>
            <end position="742"/>
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        <property type="entry name" value="Chitin-bd_dom_sf"/>
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        <property type="entry name" value="CHITIN DEACETYLASE-LIKE 5, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
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            <begin position="29"/>
            <end position="1775"/>
        </location>
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        <location>
            <begin position="55"/>
            <end position="110"/>
        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
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            <begin position="1332"/>
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        </location>
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            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <begin position="180"/>
            <end position="193"/>
        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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        </location>
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    </feature>
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        <location>
            <begin position="1173"/>
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        </location>
    </feature>
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        <location>
            <begin position="1189"/>
            <end position="1199"/>
        </location>
    </feature>
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        <location>
            <begin position="1200"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="1295"/>
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        </location>
    </feature>
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        <location>
            <begin position="1332"/>
            <end position="1341"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF96102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1775" mass="195937" checksum="BA8E56E524668291" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKG4</accession>
    <name>A0AAU9FKG4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_13322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKG4"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="117"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF96046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="283" mass="33541" checksum="B4E28AB0DCFC1847" modified="2024-11-27" version="1">MEQGSGMQVDVCPNPMLQPQTSEWVVDPYRKRSESLKIFVAALVRSWRRRGENICQLEEETQHFRDEYLQIKNSVRLSKALKDVELERNGELHLELMMYKRSFYEAQTSIYSLTTDKKKLIHEVECSKREHDELQAVADQRQIEICTALKKQLIDQQRLSQEEKAINRLRRKNADLVTEVTTLRRMQMEYKISEQCLTKELEETNERMSTIQEELNCLQDRMRIWNSNEPLEHEPQVRPKMEQQELFISNDSLQTECSKSNKAVFPMPQHVLLNALILFIKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKG6</accession>
    <name>A0AAU9FKG6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tubulin polyglutamylase TTLL4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_13490</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin--tyrosine ligase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKG6"/>
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        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000009">
        <property type="entry name" value="tubulin polyglutamylase TTLL5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF162">
        <property type="entry name" value="TUBULIN MONOGLUTAMYLASE TTLL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="69"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="190"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="69"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF96253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="977" mass="109581" checksum="8CE860E16C05566D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKG9</accession>
    <name>A0AAU9FKG9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dipeptidase</fullName>
            <ecNumber evidence="1">3.4.13.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_13508</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an L-aminoacyl-L-amino acid + H2O = 2 an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:48940"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="ChEBI" id="CHEBI:77460"/>
            <dbReference type="EC" id="3.4.13.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Peptidase M19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.13.19" evidence="1"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKG9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dipep_AS"/>
    </dbReference>
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        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008257">
        <property type="entry name" value="Pept_M19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443:SF47">
        <property type="entry name" value="DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443">
        <property type="entry name" value="MICROSOMAL DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01244">
        <property type="entry name" value="Peptidase_M19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RENAL_DIPEPTIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0482">Metalloprotease</keyword>
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    <keyword evidence="1" id="KW-0862">Zinc</keyword>
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            <end position="379"/>
        </location>
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            <end position="655"/>
        </location>
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            <begin position="319"/>
            <end position="345"/>
        </location>
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            <end position="363"/>
        </location>
    </feature>
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        <location>
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            <end position="612"/>
        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF96274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="896" mass="96862" checksum="3D2CBC45A6355E2C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKH2</accession>
    <name>A0AAU9FKH2_DROMD</name>
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        <recommendedName>
            <fullName evidence="11">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_13392</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF96135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4 11" id="KW-0430">Lectin</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ricin B lectin" evidence="12">
        <location>
            <begin position="466"/>
            <end position="597"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF96135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="617" mass="70020" checksum="94156FAF2B5E5C74" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKH3</accession>
    <name>A0AAU9FKH3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine-rich adhesin for platelets</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_13346</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13220">
        <property type="entry name" value="PH-GRAM_GRAMDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000008">
        <property type="entry name" value="GRAM domain containing 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF4">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1188"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="7">
        <location>
            <begin position="757"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="65"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="175"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="517"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="694"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="928"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1135"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="175"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="224"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="313"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKJ1</accession>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">RF1</strain>
        </source>
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAU9FKJ7_DROMD</name>
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    </gene>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="74" mass="8052" checksum="2BA1BD6F2E176FA3" modified="2024-11-27" version="1">MAKGQNPCPSLCWPLAVPKDNGSSAKGNAAPARRACNTDGATCKDITSKANICDYKEELQEEELQEEELQEKEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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    </gene>
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        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="LD20667P"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DHR-2_Lobe_B"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="478"/>
        </location>
    </feature>
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        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF96111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2065" mass="234669" checksum="7BAAF53534D6BBF0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKM6</accession>
    <name>A0AAU9FKM6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endoplasmic reticulum-Golgi intermediate compartment protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_05113</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ERGIC family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKM6"/>
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        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF25">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN 3"/>
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            <begin position="330"/>
            <end position="355"/>
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            <begin position="7"/>
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            <dbReference type="ARBA" id="ARBA00004257"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004457"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00005648"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00040493"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF96476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="372" mass="42418" checksum="8301586E4BB6B65B" modified="2024-11-27" version="1">MKFADVLRRLDAYPRTLDDFSVRTVGGAAVTIMSTSIISLLIFLEFLNYMQPALNEELFVDTTRGHKLRINLDVTLHNLACNYISLDAMDSSGDTHLRVDHDIFKHRLDLKGEPLKETPIKEIVAVSPPNKNVTCGSCYGAEHNATHCCNTCEEVLDAYRLRKWNVQVDKIEQCKGKYKRTDEDAFKEGCRIQGHLEVNRMAGSFHFAPGKSFSIRQFHIHDFQFSNVKLSHTINHLSFGEKIEFAKTHPLDGLRVDVAETKSEMFNYYLKIVPTLYMRQRDGQPIYTNQFSVTRYRKDLTDRERGMPGIFFSYELSPLMVKYAQKHSSFGHFATNCCSIIGGVFTVAGILAVLLNNSWEAIQRKLDVGKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKN3</accession>
    <name>A0AAU9FKN3_DROMD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_13425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKN3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
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        <location>
            <begin position="41"/>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
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            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
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        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF96177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="147" mass="16650" checksum="933C536F33E5633C" modified="2024-11-27" version="1">MLTIKHAERWVQQDNQKSDDSDKTQVYVRRQVNPSYWDKLKQKQSLANNGFQPEQLDSEPATPFVGTGRAIKGGFPTEAQTQVPTQPQPQLQATPMGQYNSQHQQRENNYSNTFGMVSNSTSTSRPNSSYYSNYSNSFVKGDMLLPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKS8</accession>
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        <submittedName>
            <fullName evidence="14">Probable G-protein coupled receptor 179</fullName>
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        <name evidence="14" type="ORF">DMAD_13421</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
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    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="14 15">RF1</strain>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
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        <property type="protein sequence ID" value="BFF96171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome U"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="7tmC_GPR158-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GPR158/179"/>
    </dbReference>
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        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 158-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32546:SF26">
        <property type="entry name" value="SMOG, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="8 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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            <end position="642"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="744"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="554"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="887"/>
            <end position="924"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
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        <location>
            <begin position="74"/>
            <end position="88"/>
        </location>
    </feature>
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        <location>
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            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
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            <end position="281"/>
        </location>
    </feature>
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        <location>
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            <end position="912"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF96171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="950" mass="105375" checksum="985475F82F6C5B58" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL02</accession>
    <name>A0AAU9FL02_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphodiesterase</fullName>
            <ecNumber evidence="9">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_13532</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="9">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="9"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047555">
        <property type="term" value="F:3',5'-cyclic-GMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046068">
        <property type="term" value="P:cGMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000003">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000031">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000032">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0140">cGMP</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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            <begin position="756"/>
            <end position="1080"/>
        </location>
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            <begin position="28"/>
            <end position="51"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="66"/>
            <end position="156"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="197"/>
            <end position="225"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="286"/>
            <end position="313"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="1176"/>
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            <begin position="84"/>
            <end position="105"/>
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            <begin position="106"/>
            <end position="149"/>
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            <begin position="197"/>
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            <begin position="1301"/>
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        <ligand>
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            <name>AMP</name>
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        <ligand>
            <name>Zn(2+)</name>
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            <dbReference type="EMBL" id="BFF96303.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1425" mass="157042" checksum="1860407AFD542FD3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL04</accession>
    <name>A0AAU9FL04_DROMD</name>
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        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="14" type="ORF">DMAD_13485</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="14 15">RF1</strain>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:regulation of gene expression"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Transcription factor E4F1 isoform 1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="zinc finger protein 546 isoform X1"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
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        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
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        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
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        <property type="entry name" value="ZnF_C2H2"/>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
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    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="595"/>
            <end position="623"/>
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        <location>
            <begin position="624"/>
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        <location>
            <begin position="652"/>
            <end position="679"/>
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        <location>
            <begin position="680"/>
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    </feature>
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        <location>
            <begin position="488"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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        <location>
            <begin position="503"/>
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    </feature>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF96246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="762" mass="85847" checksum="3F1E454C5183C5EE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL05</accession>
    <name>A0AAU9FL05_DROMD</name>
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        <recommendedName>
            <fullName evidence="14">Probable ATP-dependent RNA helicase DDX23</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
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        <alternativeName>
            <fullName evidence="15">DEAD box protein 23</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_13538</name>
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    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation. Independently of its spliceosome formation function, required for the suppression of incorrect R-loops formed during transcription; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">The phosphorylated form (by SRPK2) is a component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, WDR57, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Identified in the spliceosome C complex. Interacts with ERBB4. Interacts with ERCC6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX23/PRP28 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FL05"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17945">
        <property type="entry name" value="DEADc_DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000322">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000520">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057479">
        <property type="entry name" value="PRP28/DDX23-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25430">
        <property type="entry name" value="DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 21" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="20">
        <location>
            <begin position="408"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="439"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="667"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="191"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="809"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="408"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="61"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="126"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="162"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="220"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="243"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055288"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062365"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="BFF96312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="836" mass="96081" checksum="0C8B2415924E308F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL06</accession>
    <name>A0AAU9FL06_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein cappuccino</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_13557</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family. Cappuccino subfamily.</text>
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        <property type="entry name" value="Formin_Cappuccino_subfam"/>
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        <property type="entry name" value="FORMIN HOMOLOGY 2 DOMAIN CONTAINING, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920:SF7">
        <property type="entry name" value="FORMIN-G"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="FORMIN"/>
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    <dbReference type="SMART" id="SM00498">
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    <dbReference type="SUPFAM" id="SSF101447">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1089"/>
            <end position="1493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="191"/>
            <end position="219"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="408"/>
            <end position="439"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="461"/>
            <end position="482"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="521"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
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        <location>
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            <begin position="1054"/>
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            <begin position="1468"/>
            <end position="1495"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="521"/>
            <end position="547"/>
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    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="907"/>
            <end position="1014"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1058"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1079"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF96337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1520" mass="161532" checksum="4E6250FEE04B1561" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSW9</accession>
    <name>A0AAU9FSW9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Arginine-glutamic acid dipeptide repeats protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_06738</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSX7</accession>
    <name>A0AAU9FSX7_DROMD</name>
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        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
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        <name evidence="18" type="ORF">DMAD_06796</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.</text>
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        <property type="protein sequence ID" value="BFF98715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140693">
        <property type="term" value="F:molecular condensate scaffold activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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    <dbReference type="GO" id="GO:0006884">
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0140694">
        <property type="term" value="P:membraneless organelle assembly"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="CCTL2_WNK"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Kinase_OSR1/WNK_CCT"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR008271">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="11 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
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            <end position="543"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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        <source>
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        <source>
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    <sequence length="2311" mass="257242" checksum="806785907A2F216E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSX9</accession>
    <name>A0AAU9FSX9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mediator of RNA polymerase II transcription subunit 26</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ORF">DMAD_06815</name>
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    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </source>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <dbReference type="Proteomes" id="UP001500889"/>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Yamamoto D."/>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00071250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="BFF99107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="583" mass="62888" checksum="FE7D7DD5292C340C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSZ4</accession>
    <name>A0AAU9FSZ4_DROMD</name>
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        <submittedName>
            <fullName evidence="12">Protein boule</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_07094</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="term" value="P:3'-UTR-mediated mRNA stabilization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:meiotic cell cycle"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:spermatogenesis"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="RRM_DAZL_BOULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000167">
        <property type="entry name" value="protein boule-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034988">
        <property type="entry name" value="DAZ_BOULE_RRM"/>
    </dbReference>
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        <property type="entry name" value="DAZ_dom"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11176">
        <property type="entry name" value="BOULE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11176:SF57">
        <property type="entry name" value="PROTEIN BOULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51890">
        <property type="entry name" value="DAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="32"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="DAZ" evidence="11">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="190"/>
            <end position="222"/>
        </location>
    </feature>
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        <location>
            <begin position="201"/>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF99122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="246" mass="26993" checksum="CE5AA9F4A743FB3B" modified="2024-11-27" version="1">MHKIGAQPPSSAPAGGLDAPLAAPKYGTLIPNRIFVGGISGDTTEADLTRVFSAYGTVKSTKIIVDRAGVSKGYGFVTFETEQEAQRLQSDGECVVLRDRKLNIAPAIKKQPNPLQSIVATNGAVYYTTTPPAPISNIPMDQFAAAVYPPVTDFTAAGVPAIYPPSAMQYQPFYQYYSVPMQNVPTIWPQNYQENHPPPISQMASQQQPHQNQHQQQMLHPQQQPNANWMEFDDLNINLNNCGPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSZ5</accession>
    <name>A0AAU9FSZ5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcription factor AP-2-epsilon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_06808</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FSZ5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004979">
        <property type="entry name" value="TF_AP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013854">
        <property type="entry name" value="TF_AP2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812:SF17">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03299">
        <property type="entry name" value="TF_AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01748">
        <property type="entry name" value="AP2TNSCPFCT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor AP-2 C-terminal" evidence="8">
        <location>
            <begin position="241"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="22"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="22"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007770"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF98730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="465" mass="50799" checksum="86B1A460D9A13742" modified="2024-11-27" version="1">MSFLATLDASAWQERLSGHGSLGLSSSSAAYTTHSGGHTGRSGPATGHSGHSGTHGSAATMHHQSLQSDFQPPYFPPPFHHSTQSPPQQQISEYLQNHGALEYLGTDPYGQPLSSLHHAPLHHYNQLAGLRSTQDQLGIHRTHREAELQGHVSQLAHGFPYTDRRGDYSSAISAGAAHGTRLGHEHESLALHQALQNAVDDVQAPAIDDNVAFMSDLPLIKSMKSGKDAGNIGSGSPSEVFCAVPGRLSLLSSTSKYKVTIAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRLLREKLEKIGLNLPAGRRKAANVTLLTSLVEGEATHLAKDFHFVCETEFPARQLAEYIVRHQTEPNDSYRRKELILHSQQITKELMQILSQDRTTHFGTRSQHLLEPSMQRHLTHFSLITHGFGSPAIMAVLHAFQTFLNESLNYLEKLYPSNGGGMVSSSLDKSKMDNEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSZ9</accession>
    <name>A0AAU9FSZ9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha-protein kinase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_06754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FSZ9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052145">
        <property type="entry name" value="Mediator/Homeobox_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330">
        <property type="entry name" value="HOMEOBOX PROTEIN BARH-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
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    </feature>
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        <location>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF98658.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="824" mass="92815" checksum="5019366A3EF89E9D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FT87</accession>
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        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
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        </alternativeName>
    </protein>
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        <name evidence="17" type="ORF">DMAD_06775</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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        <text evidence="3">Protein modification; protein ubiquitination.</text>
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        <subcellularLocation>
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        </subcellularLocation>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0008586">
        <property type="term" value="P:imaginal disc-derived wing vein morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 12" id="KW-0833">Ubl conjugation pathway</keyword>
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        </location>
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        <location>
            <position position="968"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50004"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50020"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50237"/>
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        <source>
            <dbReference type="EMBL" id="BFF98684.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1001" mass="114314" checksum="525CFDDDA88C0C68" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FT90</accession>
    <name>A0AAU9FT90_DROMD</name>
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        <submittedName>
            <fullName evidence="11">Sodium-dependent nutrient amino acid transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_06755</name>
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    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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        <text evidence="2">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
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    </dbReference>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
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    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
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            <end position="331"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
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            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="403"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
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        <location>
            <begin position="508"/>
            <end position="527"/>
        </location>
    </feature>
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        <location>
            <begin position="602"/>
            <end position="635"/>
        </location>
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            <end position="631"/>
        </location>
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    <feature type="binding site" evidence="8">
        <location>
            <position position="63"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
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        <location>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF98661.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="816" mass="91477" checksum="616868C81CCF85A1" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FT94</accession>
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        <name evidence="6" type="ORF">DMAD_06903</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
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        </lineage>
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    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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        </subcellularLocation>
    </comment>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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</entry>
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            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
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            <end position="138"/>
        </location>
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            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="53596" checksum="9CD1C5788993BC4A" modified="2024-11-27" version="1">MNKLRSLLPGQQAPSQGSSNRHSVVLEPEIGFQITIDTAGGESAAKSSTAVPELNVHLIAARHLPSLFGFKIVQGYLIKLFPGTKRLDSTIQTNTWPKFNENFKFPLVPDIKSSLKHGSARDMLSRRSTNNHQTTAPATNHVPEPDELFAGQFVVFTVYALLELPAASFNRFNKTYRSLKERSSNFVQRLVDSSASGTPNESKPEALGKPNEGKSKKKSENPRDAMPPLTISESRRNIGSVTCYLEPKLFKRNARTGCYSSEELWLPIKDITTTVSKTTSNNNNNTLNNSPKGVVELALEVRDLGEDGSMPVPATQPPTPAAGPASNNWQRMTAEMKRKMGMSNASHVESGRYVLRVTTTRMRCSNKVKDELEATASAGVYVKTTAFEHGIYIDAWKSPNFFPSLSTKWDDGTDVNEGTITIPLRSLDQLENIVVRVTLATKKMGKKLVLGTVLIGGDRAGDTGSEQLRLIRETPPGERVAAWHCYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTD1</accession>
    <name>A0AAU9FTD1_DROMD</name>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="14">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
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            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="19">
        <location>
            <begin position="645"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="974"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="988"/>
            <end position="999"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030680"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00051911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF99304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1105" mass="122816" checksum="B3ED227801CEE4DC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4W9</accession>
    <name>A0AAU9G4W9_DROMD</name>
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        <recommendedName>
            <fullName evidence="2">Iron-sulfur cluster assembly 1 homolog, mitochondrial</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">DMAD_02156</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the HesB/IscA family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Iron-binding protein IscA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="iron-sulfur cluster assembly accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072:SF41">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 1 HOMOLOG, MITOCHONDRIAL"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fe-S_biosyn"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <feature type="domain" description="Core" evidence="3">
        <location>
            <begin position="26"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00039743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG02722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="130" mass="14116" checksum="DAD2337A661A72CB" modified="2024-11-27" version="1">MATRVVATATVRAVKGRKLIPTRAALTLTPAAVNRIKSLLQDKPDFIGLKVGVRQRGCNGLSYTLDYASSKDKLDEEVVQDGVKVFIDKKAQLSLLGTEMDFVESKLSSEFVFNNPNIKGTCGCGESFSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4X7</accession>
    <name>A0AAU9G4X7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Chaoptin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_02190</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="entry name" value="GH01279p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000770">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF171">
        <property type="entry name" value="LEUCINE RICH REPEAT NEURONAL 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="31"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1311"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1090"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1402"/>
            <end position="1436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1468"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1100"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1413"/>
            <end position="1436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1470"/>
            <end position="1479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG02776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1513" mass="170194" checksum="947FC50E90B51938" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4Y3</accession>
    <name>A0AAU9G4Y3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Potassium voltage-gated channel protein eag</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_02225</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G4Y3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.260:FF:000003">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000009">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000009">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003949">
        <property type="entry name" value="K_chnl_volt-dep_EAG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01464">
        <property type="entry name" value="EAGCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="367"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="17">
        <location>
            <begin position="43"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="18">
        <location>
            <begin position="113"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="16">
        <location>
            <begin position="594"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="737"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="777"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="877"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="974"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1043"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1127"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="974"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="987"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1055"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1070"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1138"/>
            <end position="1156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00023065"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFG02826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1182" mass="126733" checksum="1D889B7654D97BB7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4Z7</accession>
    <name>A0AAU9G4Z7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribonuclease H1</fullName>
            <shortName evidence="9">RNase H1</shortName>
            <ecNumber evidence="9">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_02760</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the RNase H family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="9"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G4Z7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09280">
        <property type="entry name" value="RNase_HI_eukaryote_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.970.10:FF:000002">
        <property type="entry name" value="Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000097">
        <property type="entry name" value="Ribonuclease H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.970.10">
        <property type="entry name" value="Ribonuclease H1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050092">
        <property type="entry name" value="RNase_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017067">
        <property type="entry name" value="RNase_H1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011320">
        <property type="entry name" value="RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037056">
        <property type="entry name" value="RNase_H1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10642">
        <property type="entry name" value="RIBONUCLEASE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10642:SF31">
        <property type="entry name" value="RIBONUCLEASE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01693">
        <property type="entry name" value="Cauli_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036852">
        <property type="entry name" value="Ribonuclease_H1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="10">
        <location>
            <begin position="161"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR036852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG03527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="317" mass="35415" checksum="678BD5C09D205B09" modified="2024-11-27" version="1">MAYYAVASGRKAGIYDTWAQCEQQVKGFKNAVYKKFNTRQEAEQFINIKSTYAPHEVAVPLGQKQPLPASWSTTKKQEPQYTEAWPDEDHDLVEDELNAAMNEVEGDPKPTSSSILIYGTTQKRKLDTSDDRQKKVPRHVSRVADAAGIKHVGEFEFEIDAEGYVIVYTDGSCIGNGRKGACAGYGVYFGDNHPLNAAQPVSGRVTNNVGEIQAAIHAIKTALDLGIGKLCIYTDSQFLINSITLWVQGWKRRGWMLANNQPVKNVVDFKELDELLQNKDIKVKWNYVEAHKGINGNEMADKLARQGSALYKEQNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4Z8</accession>
    <name>A0AAU9G4Z8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MAP kinase-activating death domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_02358</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
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            <dbReference type="EMBL" id="BFG02752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="983" mass="107977" checksum="BF21BFABD7CA396C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G591</accession>
    <name>A0AAU9G591_DROMD</name>
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        <submittedName>
            <fullName evidence="11">Trypsin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_02866</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
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        <property type="protein sequence ID" value="BFG03668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Serine proteinase stubble"/>
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    </dbReference>
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        <property type="entry name" value="Trypsin-like serine proteases"/>
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    </dbReference>
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        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
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        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
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        <property type="entry name" value="ACROSIN-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="HYALIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
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        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
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    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043583290" evidence="9">
        <location>
            <begin position="21"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="134"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="61"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="68"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG03668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="379" mass="41102" checksum="204DFCE745329AF5" modified="2024-11-27" version="1">MSRASIGLVICLLLVLLGLAQTFAEEQQQPARPEATPVSPLLKQSQNTFIQWVLSLLPQRPGGTDGESATLATLSSSSTKPEPASSSTTTSTTPAPSSSTTTTRRATTAAPPTLTPARNCTDCVCGIANIQKRIVGGQETEVHQYPWVAMLLYGGRFYCAASLLNDQFLLTASHCVYGFRRERITVRLLDHNRKMSHTQKIDRNVAEVITHPKYNARNYDNDIAIIKLDEPVEFDEVLHPVCMPTPGRSFKGETGIVTGWGALKVGGPTSDTLQEVQVPILSQDACRKSRYGNKITDNMLCGGYDEGGKDSCQGDSGGPLHIVASGTREHQIAGVVSWGEGCAKSGYPGVYARVNRYGTWIKNLTKQACLCQQETKKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G592</accession>
    <name>A0AAU9G592_DROMD</name>
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        <submittedName>
            <fullName evidence="14">WD repeat-containing protein 19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_02830</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035721">
        <property type="term" value="P:intraciliary retrograde transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000845">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056168">
        <property type="entry name" value="TPR_IF140/IFT172/WDR19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR056170">
        <property type="entry name" value="Znf_IFT121-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14920">
        <property type="entry name" value="OSMOTIC AVOIDANCE ABNORMAL PROTEIN 1/WD REPEAT MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14920:SF0">
        <property type="entry name" value="WD REPEAT DOMAIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23389">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23145">
        <property type="entry name" value="Zf_2nd_IFT121"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR19 first beta-propeller" evidence="12">
        <location>
            <begin position="19"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="WDR19 WD40 repeat" evidence="10">
        <location>
            <begin position="360"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="IF140/IFT172/WDR19 TPR" evidence="13">
        <location>
            <begin position="660"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="IF140/IFT172/WDR19 TPR" evidence="13">
        <location>
            <begin position="858"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="domain" description="IFT121-like zinc finger" evidence="11">
        <location>
            <begin position="1301"/>
            <end position="1346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF15911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF24762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFG03617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1366" mass="153428" checksum="4ABB290D6C23C9FE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G598</accession>
    <name>A0AAU9G598_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serine-rich adhesin for platelets</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_02616</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03384">
        <property type="entry name" value="PAP2_wunen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000036">
        <property type="entry name" value="Putative phosphatidate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF197">
        <property type="entry name" value="FI04477P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="192"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG06275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="363" mass="41292" checksum="C172D691CE97C961" modified="2024-11-27" version="1">MSKESSVSETTPLRRQPSQLSAESEANVANTRQATTVVSLVDNNNLSSNNNHYSNLVEVRLQAAEENQQQQQQQQGENMDTNKRILCRVGLDILILLCVGFPILLFFLLGDPYKRGFFCDDESLRHPFHDSTVRNWMLYFIGVLIPVGVILTVEVLISRRKAKLDNGNATSRRYVFMGYELPNWLIECYKKVGIYGFGAAVSQLTTDIAKYSIGRLRPHFIAVCQPVLTDGSTCDDPQNANKYIQEFTCRGLGSSARMLKEMRLSFPSGHSSFTFFAMVYMALYLQARMTWHGSKLLRHLLQFMFIMIAWYTALSRVSDYKHHWSDVLAGALIGSTCALVVSFCLPKNWFNWHTVWPAKGKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFA1</accession>
    <name>A0AAU9GFA1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14114">
        <property type="entry name" value="STKc_Twitchin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000321">
        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000440">
        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000097">
        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000642">
        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000614">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001845">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001892">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000460">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000504">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000505">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000147">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002083">
        <property type="entry name" value="Protein CBR-UNC-22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000160">
        <property type="entry name" value="Titin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000003">
        <property type="entry name" value="Titin isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000012">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000127">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001003">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000249">
        <property type="entry name" value="twitchin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000056">
        <property type="entry name" value="twitchin isoform X4"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000936">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000006">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000775">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000831">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001034">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001164">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000567">
        <property type="entry name" value="Uncharacterized protein, isoform G"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002180">
        <property type="entry name" value="Uncharacterized protein, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000466">
        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000876">
        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001033">
        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000935">
        <property type="entry name" value="Uncharacterized protein, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001001">
        <property type="entry name" value="Uncharacterized protein, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000051">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000553">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000559">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000748">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001016">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="76"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF151">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="40"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="37"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    <sequence length="853" mass="92663" checksum="ECFFAAFDC1802187" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFE8</accession>
    <name>A0AAU9GFE8_DROMD</name>
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        <recommendedName>
            <fullName evidence="2">Kinesin-like protein unc-104</fullName>
        </recommendedName>
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        <name evidence="16" type="ORF">DMAD_04956</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for presynaptic maturation, has a role in axonal transport of dense-core vesicles carrying synaptic vesicle precursors, components required for the morphological transformation of axonal growth cones to mature boutons.</text>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome E"/>
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        <property type="term" value="C:dendrite"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="C:microtubule"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:microtubule motor activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:anterograde synaptic vesicle transport"/>
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        <property type="entry name" value="Kinesin_motor_dom_sf"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="PH-like_dom_sf"/>
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        <property type="entry name" value="PH_domain"/>
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    <dbReference type="InterPro" id="IPR049780">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
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        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF10">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF3694"/>
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        <property type="entry name" value="KIF1B"/>
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        <property type="entry name" value="Kinesin"/>
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        <property type="entry name" value="Kinesin_assoc"/>
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        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
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        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="KISc"/>
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        <property type="entry name" value="PH_DOMAIN"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
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        <location>
            <begin position="3"/>
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        <location>
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    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    </feature>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00056070"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50067"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG06447.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
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</entry>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <strain evidence="2 3">RF1</strain>
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            <end position="93"/>
        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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    </feature>
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    </evidence>
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</entry>
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        <reaction evidence="19">
            <text>Ca(2+)(in) + 3 Na(+)(out) = Ca(2+)(out) + 3 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:69955"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC8 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EYN9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005432">
        <property type="term" value="F:calcium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2030:FF:000024">
        <property type="entry name" value="sodium/calcium exchanger 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051171">
        <property type="entry name" value="CaCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038081">
        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003644">
        <property type="entry name" value="Calx_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004836">
        <property type="entry name" value="Na_Ca_Ex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032452">
        <property type="entry name" value="Na_Ca_Ex_C-exten"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00845">
        <property type="entry name" value="caca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878:SF65">
        <property type="entry name" value="NA_CA-EXCHANGE PROTEIN, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878">
        <property type="entry name" value="SODIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03160">
        <property type="entry name" value="Calx-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16494">
        <property type="entry name" value="Na_Ca_ex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01259">
        <property type="entry name" value="NACAEXCHNGR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00237">
        <property type="entry name" value="Calx_beta"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141072">
        <property type="entry name" value="CalX-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 21" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043684078" evidence="21">
        <location>
            <begin position="27"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="782"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="815"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="854"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="885"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="925"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="22">
        <location>
            <begin position="426"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="22">
        <location>
            <begin position="543"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BFF90870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="951" mass="106605" checksum="0D678BC81E434F67" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYP2</accession>
    <name>A0AAU9EYP2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Low-density lipoprotein receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_07726</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0043235">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:hormone binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000105">
        <property type="entry name" value="Lipophorin receptor 1, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000015">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000034">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000008">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000113">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000030">
        <property type="entry name" value="Sortilin related receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF14">
        <property type="entry name" value="MEGALIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
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            <begin position="1"/>
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        </location>
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            <end position="543"/>
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        <location>
            <begin position="743"/>
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        <location>
            <begin position="755"/>
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    <feature type="disulfide bond" evidence="16">
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    <feature type="disulfide bond" evidence="16">
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    <feature type="disulfide bond" evidence="16">
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    <feature type="disulfide bond" evidence="16">
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        <location>
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        <location>
            <begin position="300"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="868" mass="96554" checksum="8B27BD9EA7F1A99F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYR6</accession>
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        <submittedName>
            <fullName evidence="4">Dipeptidyl peptidase 9</fullName>
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        <name evidence="4" type="ORF">DMAD_07747</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Diptera</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4 5">RF1</strain>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <location>
            <begin position="425"/>
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        <location>
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        <location>
            <begin position="55"/>
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    </feature>
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        <location>
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            <end position="324"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF88851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1112" mass="123660" checksum="F9E07F697FF37167" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYS3</accession>
    <name>A0AAU9EYS3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Phosphatidylinositol-binding clathrin assembly protein LAP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_08306</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Binds clathrin and phosphatidylinositol 4,5-bisphosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PICALM/SNAP91 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:extrinsic component of presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16985">
        <property type="entry name" value="ANTH_N_AP180"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol-binding clathrin assembly protein LAP"/>
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    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
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            <begin position="16"/>
            <end position="159"/>
        </location>
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            <begin position="581"/>
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            <begin position="661"/>
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            <begin position="683"/>
            <end position="693"/>
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            <dbReference type="ARBA" id="ARBA00023176"/>
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        <source>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="703" mass="74781" checksum="F7804F197E99E815" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYS4</accession>
    <name>A0AAU9EYS4_DROMD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_08689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome O"/>
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        <location>
            <begin position="148"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="294"/>
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    </feature>
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        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF90099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="309" mass="34111" checksum="188C64F7DCAEC86D" modified="2024-11-27" version="1">MLKNPKIVKIPPATVKSPRPNRAAAKSFDVAQVCSVDLESDGGVRLPPLTLESRIQPEDVADARFRLVEARSKPVMLSDRQFDVHMKRLVKVFMSEVSITRDMREITSKRSLQPADRLLLQDLEILKGKLRQERCSLVVTLSKDSRHRCQQSAATDIAPKASNQSQVRDEKPPESADEPKPSTSGMSKILKRPFQSRKRGGAQPKVASNPPIICSNIETPKELSQKLMEKLAKACESPAAPDETTRKLKQRFAITTDSNEDKDASQNAPKIDPTNAPAAAPVSPATEATSEALSRKSSDIFYDFPVAIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYU8</accession>
    <name>A0AAU9EYU8_DROMD</name>
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        <recommendedName>
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        </recommendedName>
        <alternativeName>
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        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Baculoviral IAP repeat-containing protein 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Ubiquitin-conjugating BIR domain enzyme apollon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="ORF">DMAD_09354</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="31 32">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Microtubule organizing center</location>
            <location evidence="4">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="24">Belongs to the BIRC6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23810">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000014">
        <property type="entry name" value="Baculoviral IAP repeat-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000001">
        <property type="entry name" value="baculoviral IAP repeat-containing protein 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022103">
        <property type="entry name" value="BIRC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116">
        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116:SF39">
        <property type="entry name" value="BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12356">
        <property type="entry name" value="BIRC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="23" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="22" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-0967">Endosome</keyword>
    <keyword evidence="20" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="21" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="19" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="17" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0853">WD repeat</keyword>
    <keyword evidence="18" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="UBC core" evidence="30">
        <location>
            <begin position="4572"/>
            <end position="4739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="498"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="650"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="979"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="2308"/>
            <end position="2344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="2899"/>
            <end position="2928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="3628"/>
            <end position="3670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="4231"/>
            <end position="4291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="4885"/>
            <end position="4924"/>
        </location>
    </feature>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        <name evidence="10" type="ORF">DMAD_08712</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
    <sequence length="1159" mass="129035" checksum="B0B20B87CABF8179" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="6">TBC1 domain family member 4</fullName>
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        <name evidence="6" type="ORF">DMAD_09846</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6 7">RF1</strain>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <location>
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        <location>
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        <location>
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</entry>
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        <property type="match status" value="1"/>
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    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0630">Potassium</keyword>
    <keyword evidence="7" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
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    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0851">Voltage-gated channel</keyword>
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        <location>
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    <feature type="region of interest" description="Disordered" evidence="17">
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        <location>
            <begin position="784"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
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        <source>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
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            <dbReference type="SAM" id="Phobius"/>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
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    <sequence length="1222" mass="135578" checksum="AC18F697A73361B7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZ58</accession>
    <name>A0AAU9EZ58_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Thiamine pyrophosphokinase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_07865</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate (TPP) utilizing UTP and therefore links the biosynthesis of TPP to pyrimidines metabolism. By producing thiamine pyrophosphate, a cofactor of the mitochondrial pyruvate dehydrogenase indirectly regulates pyruvate oxidation and lipogenesis. Although it can also catalyze thiamine phosphorylation using ATP and CTP in vitro, it does so with significantly lower efficiency and without physiological relevance evidence.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>thiamine + UTP = thiamine diphosphate + UMP + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18385"/>
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            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
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        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thiamine pyrophosphokinase family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF89016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:thiamine diphosphokinase activity"/>
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        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thiamine pyrophosphokinase"/>
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        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
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        <property type="entry name" value="Thiamin pyrophosphokinase, thiamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thi_PPkinase"/>
    </dbReference>
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        <property type="entry name" value="Thiamin_PyroPKinase_B1-bd"/>
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        <property type="entry name" value="TPK_B1-bd_sf"/>
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        <property type="entry name" value="TPK_catalytic"/>
    </dbReference>
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        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Thiamin pyrophosphokinase, substrate-binding domain"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamin pyrophosphokinase thiamin-binding" evidence="11">
        <location>
            <begin position="210"/>
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        </location>
    </feature>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00074758"/>
        </source>
    </evidence>
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            <dbReference type="SMART" id="SM00983"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF89016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="296" mass="33035" checksum="70D92672AE664469" modified="2024-11-27" version="1">MAIADGDNRSLCTKTTIGFRQQMDHTVPPKEFKWTPGNMINENFKLGDNGHVCVVLNRQIQVPAHVVKSLWTNAAVRCAVDGGSNHWREFLLGQAVAKRPKGAPATAPLEPLDVITGDFDSITEETVDFFKTTPKIHTPDQDATDFTKAISVLQPVMAQRKIHDVVVFHDTSGRLDQVMANLNTLYKAQKDNCNVFLLSGDSITWLLRPGKHTIQVPLDLVKTQRWCSLMPVGSMAHNVTTTGLKWNLYHTQMEFGGMVSTSNTYSTEFVQVETDANLIWSMGAYDFEDKLRQAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZ60</accession>
    <name>A0AAU9EZ60_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Polypyrimidine tract-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_09445</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EZ60"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="RRM1_PTBP1_hnRNPL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12693">
        <property type="entry name" value="RRM2_PTBP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12423">
        <property type="entry name" value="RRM3_PTBP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000341">
        <property type="entry name" value="Hephaestus, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000036">
        <property type="entry name" value="polypyrimidine tract-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000018">
        <property type="entry name" value="Polypyrimidine tract-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="HnRNP-L/PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055204">
        <property type="entry name" value="HNRNPL_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021790">
        <property type="entry name" value="PTBP1-like_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01649">
        <property type="entry name" value="hnRNP-L_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22976">
        <property type="entry name" value="RRM_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11835">
        <property type="entry name" value="RRM_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="95"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="248"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="437"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="554"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF91080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="631" mass="68202" checksum="756BD37025314CC0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZ66</accession>
    <name>A0AAU9EZ66_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bromodomain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_10073</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EZ66"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043695228" evidence="2">
        <location>
            <begin position="17"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="459"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="603"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="462"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="498"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="609"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF91900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="660" mass="73712" checksum="4D414009AEB836E5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F2A2</accession>
    <name>A0AAU9F2A2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA replication licensing factor MCM5</fullName>
            <ecNumber evidence="13">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_09904</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="13"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F2A2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000005">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000006">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008048">
        <property type="entry name" value="MCM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054125">
        <property type="entry name" value="MCM5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF42">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21933">
        <property type="entry name" value="MCM5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01661">
        <property type="entry name" value="MCMPROTEIN5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="329"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF91679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="676" mass="75910" checksum="6FD2BDED571981CC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F2C7</accession>
    <name>A0AAU9F2C7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Hemicentin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_09944</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F2C7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278:SF25">
        <property type="entry name" value="GH14967P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278">
        <property type="entry name" value="SIDESTEP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043560712" evidence="8">
        <location>
            <begin position="24"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="620"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="37"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="142"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="248"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="347"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="444"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="656"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="812"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="660"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="681"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="812"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="829"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF91724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="909" mass="100069" checksum="54D083B57C038127" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F2E8</accession>
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        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_10091</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF91929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9F2E8"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004674">
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000602">
        <property type="entry name" value="serine/threonine-protein kinase Nek9"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
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    </dbReference>
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        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
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        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR051997">
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        <property type="entry name" value="PROTEIN CBG16200"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR44535:SF5">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 16" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="110"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="15">
        <location>
            <begin position="475"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="15">
        <location>
            <begin position="528"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="15">
        <location>
            <begin position="580"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="15">
        <location>
            <begin position="684"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFF91929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="843" mass="91909" checksum="88A04B8C7429D2A3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCA1</accession>
    <name>A0AAU9FCA1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Proton-coupled amino acid transporter-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_11190</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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                <person name="Yamamoto D."/>
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</entry>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCF2</accession>
    <name>A0AAU9FCF2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Haloacid dehalogenase-like hydrolase domain-containing protein 2</fullName>
            <ecNumber evidence="5">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Phospholysine phosphohistidine inorganic pyrophosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_11195</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Phosphatase that hydrolyzes imidodiphosphate, 3-phosphohistidine and 6-phospholysine. Has broad substrate specificity and can also hydrolyze inorganic diphosphate, but with lower efficiency.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>diphosphate + H2O = 2 phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="5"/>
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        <property type="protein sequence ID" value="BFF93325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FCF2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07509">
        <property type="entry name" value="HAD_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000051">
        <property type="entry name" value="Phospholysine phosphohistidine inorganic pyrophosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
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        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006355">
        <property type="entry name" value="LHPP/HDHD2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01458">
        <property type="entry name" value="HAD-SF-IIA-hyp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF46">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF93325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="255" mass="27560" checksum="878C807E123454F9" modified="2024-11-27" version="1">MSIKAALIDLSGTLHVEDDPTPNAVEALARLRDSGVAVKFVTNTTKDSKGTLHERLCRIGFQLDSSEIYSSLSAAVTFVQSGKLNPYYLLSEDARQDFPPEDPNRCPDSVVVGLAPKAFNYEQMNKAFNVLLQNKEHKLVAIHQGKYYKRSDGLALGPGCFVKGLEYATGCTAELIGKPNPYFFKGALDGLDPSFCVMIGDDANDDIGGAMAMGLQGILVKTGKYLPDVALSPPPTALVENFAEAVDWILKKNLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCF3</accession>
    <name>A0AAU9FCF3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Serine-rich adhesin for platelets</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_11232</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045216">
        <property type="term" value="P:cell-cell junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058586">
        <property type="entry name" value="Ajm-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038825">
        <property type="entry name" value="Apical_junction"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21517">
        <property type="entry name" value="APICAL JUNCTION COMPONENT 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21517:SF3">
        <property type="entry name" value="APICAL JUNCTION COMPONENT 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ajm-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="6">
        <location>
            <begin position="2007"/>
            <end position="2050"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        </source>
    </evidence>
    <sequence length="2340" mass="252666" checksum="7669B7BA0E6971A5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKP4</accession>
    <name>A0AAU9FKP4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein cappuccino</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_13557</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family. Cappuccino subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001265">
        <property type="entry name" value="Formin_Cappuccino_subfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920">
        <property type="entry name" value="FORMIN HOMOLOGY 2 DOMAIN CONTAINING, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920:SF7">
        <property type="entry name" value="FORMIN-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00828">
        <property type="entry name" value="FORMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="4">
        <location>
            <begin position="917"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="236"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="349"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="700"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="732"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="882"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1296"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="349"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="735"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="886"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="907"/>
            <end position="924"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF96338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1348" mass="142863" checksum="82F151393208D5AE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKQ4</accession>
    <name>A0AAU9FKQ4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dedicator of cytokinesis protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_13371</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08696">
        <property type="entry name" value="C2_Dock-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11695">
        <property type="entry name" value="DHR2_DOCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.740:FF:000002">
        <property type="entry name" value="Dedicator of cytokinesis protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.410:FF:000002">
        <property type="entry name" value="Dedicator of cytokinesis protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037808">
        <property type="entry name" value="C2_Dock-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021816">
        <property type="entry name" value="DOCK_C/D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317:SF76">
        <property type="entry name" value="LD20667P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11878">
        <property type="entry name" value="DOCK_C-D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 DOCK-type" evidence="5">
        <location>
            <begin position="591"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="6">
        <location>
            <begin position="1610"/>
            <end position="2045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="415"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF96110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2071" mass="235326" checksum="FD638030EF6BC66A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKQ6</accession>
    <name>A0AAU9FKQ6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pericentrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_05131</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028745">
        <property type="entry name" value="AKAP9/Pericentrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019528">
        <property type="entry name" value="PACT_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44981">
        <property type="entry name" value="PERICENTRIN-LIKE PROTEIN, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44981:SF2">
        <property type="entry name" value="PERICENTRIN-LIKE PROTEIN, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10495">
        <property type="entry name" value="PACT_coil_coil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pericentrin/AKAP-450 centrosomal targeting" evidence="8">
        <location>
            <begin position="2699"/>
            <end position="2768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="17"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="234"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="320"/>
            <end position="413"/>
        </location>
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        <property type="entry name" value="CUGBP Elav-like family member 1 isoform 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CUGBP Elav-like family member 2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034196">
        <property type="entry name" value="CELF1/2_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="129"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="216"/>
            <end position="297"/>
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    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="499"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="24"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="29"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009621"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF96392.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="584" mass="61981" checksum="738C22984033878E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKR1</accession>
    <name>A0AAU9FKR1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">Nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="5 16">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_13569</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate. Helps prevent cellular oxidative stress via its role in NAD biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + nicotinate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <comment type="PTM">
        <text evidence="16">Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the NAPRTase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="5 16"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKR1"/>
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        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01570">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.140.10:FF:000002">
        <property type="entry name" value="Nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.140.10:FF:000004">
        <property type="entry name" value="Nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000155">
        <property type="entry name" value="Nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000173">
        <property type="entry name" value="Nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.140.10">
        <property type="entry name" value="nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041525">
        <property type="entry name" value="N/Namide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041619">
        <property type="entry name" value="NAPRTase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040727">
        <property type="entry name" value="NAPRTase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006405">
        <property type="entry name" value="Nic_PRibTrfase_pncB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01513">
        <property type="entry name" value="NAPRTase_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098:SF1">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04095">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17956">
        <property type="entry name" value="NAPRTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17767">
        <property type="entry name" value="NAPRTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 16" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nicotinate phosphoribosyltransferase N-terminal" evidence="18">
        <location>
            <begin position="29"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate/nicotinamide phosphoribosyltransferase" evidence="17">
        <location>
            <begin position="177"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate phosphoribosyltransferase C-terminal" evidence="19">
        <location>
            <begin position="432"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF17767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF17956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF96353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="556" mass="62321" checksum="F6DED3DEA40CF67D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKR2</accession>
    <name>A0AAU9FKR2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">P protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_13415</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKR2"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01116">
        <property type="entry name" value="P_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051475">
        <property type="entry name" value="Diverse_Ion_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43568">
        <property type="entry name" value="P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43568:SF1">
        <property type="entry name" value="P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="324"/>
        </location>
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    </dbReference>
    <dbReference type="FunFam" id="3.10.390.10:FF:000002">
        <property type="entry name" value="Putative ski oncogene"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.20:FF:000002">
        <property type="entry name" value="SKI-like oncogene a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.390.10">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.20">
        <property type="entry name" value="Ski"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014890">
        <property type="entry name" value="c-SKI_SMAD4-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010919">
        <property type="entry name" value="SAND-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003380">
        <property type="entry name" value="SKI/SNO/DAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037000">
        <property type="entry name" value="Ski_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023216">
        <property type="entry name" value="Tscrpt_reg_SKI_SnoN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10005">
        <property type="entry name" value="SKI ONCOGENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10005:SF25">
        <property type="entry name" value="SNO ONCOGENE, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08782">
        <property type="entry name" value="c-SKI_SMAD_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02437">
        <property type="entry name" value="Ski_Sno_DHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01046">
        <property type="entry name" value="c-SKI_SMAD_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63763">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="c-SKI SMAD4-binding" evidence="2">
        <location>
            <begin position="247"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF96140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="358" mass="39392" checksum="2E4B0658B00349CC" modified="2024-11-27" version="1">MTEYVTPNISSVLKKYQTSATKSLQGPGHALAVAAAAAVAAAGIGGGCSSLEVASTNGGGGVGIRSESPTCGGKVQQPPSPGIPLIKKEILSSPEPHELQHHHRDAASPQIYATPATPPRELSQPILSVADAGCGELYETRLEGKTIGCFSVGGEMRLCLPQFLNNVLNDFSLEQINRIFDELGIYCSQCTPDQLVEFKAAKILPSDVKASGLITRTDAERLCAALLHRSDRNSYIPIESLAKGALSFHVYHKCFGKCEGICTPEMYSYQKPTCIKCLECDGWFSPQKFVGHVHRKFENQTCHWGFDSRNWHDYLHVALDVENREKYQIILDQLKEVELKEMHKAQLEMDHKKRKVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKU5</accession>
    <name>A0AAU9FKU5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Round spermatid basic protein 1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_13491</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the round spermatid basic protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026306">
        <property type="entry name" value="RSBN1/Dpy-2/CEP530"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354:SF11">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354">
        <property type="entry name" value="ROUND SPERMATID BASIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="325"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="878"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="998"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1270"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1427"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="89"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="357"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="370"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="391"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="890"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="945"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="973"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1003"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1066"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1104"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1128"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1270"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1430"/>
            <end position="1474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF96255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1474" mass="157675" checksum="113ED5F1EB8E0523" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKV6</accession>
    <name>A0AAU9FKV6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">F-box/LRR-repeat protein 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_13410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKV6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22126">
        <property type="entry name" value="F-box_FBXL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000574">
        <property type="entry name" value="Jetlag, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF247">
        <property type="entry name" value="GH16156P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="1">
        <location>
            <begin position="127"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF96155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="311" mass="35080" checksum="F3CEF5F73B253969" modified="2024-11-27" version="1">MCSLHVEEEAHLAAMGGNGDGTRTPSPRPRSHEANIALFEICWEDVLLPHMSTYLSLKDLFNLRCCSQTARRFAEASLEKRQELNFSGNNTSNIELAFKVVARCCRRLEVLHLACCKWLTDELLLPLLTNNKHRLCAVNLNECVNITALSLQPIIVECKDLRILKLSKCQWLTTGAVDALTLHQSKLVEFDISHCGAIGERCLIIFFRKLNKLTVLSLAHTPSVTDQVLIQIGNYCRELEHINLMGCVAISDYGVHALTKTCTRLQSLMVQRCPLVTERVLAPLRGRVHLDRPSMGYNLQPSQNHRLFLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKW0</accession>
    <name>A0AAU9FKW0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DMAD_05177</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKW0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF96566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="136" mass="15319" checksum="60EFB1D1C4664264" modified="2024-11-27" version="1">MFASTGVYAPQPGSCRQLKHTIADSTYKGIMIHRSMYLDVFGEQEANKPPSPVNLCIAIALPPKNRKLQYVILGTDHKQYVVIYICHYLKATKSHEEMINTYTAEKKSSKETLVGISEAFTKNGVNETLQLMLCDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKW1</accession>
    <name>A0AAU9FKW1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptide-methionine (S)-S-oxide reductase</fullName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Peptide-methionine (S)-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05095</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKW1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1060.10:FF:000004">
        <property type="entry name" value="Peptide methionine sulfoxide reductase A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049006">
        <property type="entry name" value="MsrA_helical"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774:SF1">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE MSRA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20939">
        <property type="entry name" value="MsrA_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="5">
        <location>
            <begin position="40"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Selenoprotein methionine sulfoxide reductase A helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF96452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="245" mass="27890" checksum="68532B2B8F44E343" modified="2024-11-27" version="1">MFAHFFRNFKARKVQYRTVRNEQKELSILPVHHVNIPKATATFGMGCFWGAESLYGATRGVLRTTVGYAGGSSELPTYRKLGDHTEVLEIDYDPTVISFKELLDLFWNNHEYGLTTPIKRQYASLILYHDDEQKKMAVTSKLEEQERRAPELITTEVAPKVNFYAAEAYHQKYRLQGHKDLATSLNLSPTLLQSSYVATKLNGYLAGVGGMEQFKSEVDTLGLTPTQRQYCSYHVEQNEGQGLYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL07</accession>
    <name>A0AAU9FL07_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein FAM92A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_13593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FL07"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009602">
        <property type="entry name" value="CBAR/FAM92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21223">
        <property type="entry name" value="CBY1-INTERACTING BAR DOMAIN-CONTAINING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21223:SF2">
        <property type="entry name" value="CBY1-INTERACTING BAR DOMAIN-CONTAINING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06730">
        <property type="entry name" value="FAM92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF96384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="412" mass="46618" checksum="7DD910E7ABC67BB9" modified="2024-11-27" version="1">MFRRGKLSFLNTKDDRIKVINERITLTERHLMEICSAFALVTRKMAKYRDSYDELAKGVKGYADDEEINESLSTGLKSFTNAVTIMGDYMDINVHRLEHKIVNEMSQFEQLCKSTRDNLRLAVIARDKEVLRQRQMLEIKSKFAANNSAADSELFKAKLEVARTNKEIDEIIGNFEQRKLRDIKQLLGDFILISMKQHTKALEVLSASYYDIGSIDERDDYLEFQKLMKTKEEPTTLKGTLKKGLRSQSMDSLDHEQLVSPLKRHQKLSRSSKNLAGAGIAHGNKGEEQQEQETDEQEDEEVDEDDDDEEETEQSGEDDEGEETGSASDDEREPAAPDAPPAPRENVFGVKPRASSSKEMSNPSMAPLKPIRQSVKHPFKAVAATHVKRRRAGRGRSSAGRLVTVVRSKPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL08</accession>
    <name>A0AAU9FL08_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bestrophin homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05108</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Forms chloride channels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the anion channel-forming bestrophin (TC 1.A.46) family. Calcium-sensitive chloride channel subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FL08"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000615">
        <property type="entry name" value="Bestrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021134">
        <property type="entry name" value="Bestrophin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10736">
        <property type="entry name" value="BESTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10736:SF65">
        <property type="entry name" value="BESTROPHIN 1, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01062">
        <property type="entry name" value="Bestrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0868">Chloride</keyword>
    <keyword evidence="6" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="6" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF96469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="504" mass="58946" checksum="BCEBF32B74B7DD12" modified="2024-11-27" version="1">MTVTYTANVNTSKVISNFLKLLFRWRGSIYKLVWIDLVIFFGLYCLVALSYRALMTENAQKVFEGIVYYCSTHGNLLPLSFVLGFYVSIVMVRWWQQYTTIPWPDSIAILVSSSIQGFDDRARAMRRTILRYVCLCQVIVFTMISPNVKKRFPTYSQIIESGLLLENEKAIIENLDVAFPHYPKHWMPIVWAASIVMRARKENKIRDDYAVKTIIDELNKFRGYCGFLLYYDWVSVPLVYTQVVTLATYTFFLFSVIGQQWTQVRETGSEPNRVVQWFPFLTILQFFFYMGWLKVAESLINPFGEDDDDFELNWMIDRNLTVSYLIVDEMHQEHPELVKDQYWDEVFPNELPYAEDAKRTGPPEASTAKLDYNKVQRFYISRDSTTTNRSGRDNFLNHILDRQSTSASFADYQHQMPSRLTFNEPTLADSEEEITCLRRPSRSGSASLNFDELIEQRHQERRERMRHRFLNMRSTHTRTGPSGGYTVTVLRAPSEDALGEEENP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL10</accession>
    <name>A0AAU9FL10_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein cappuccino</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_13557</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family. Cappuccino subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FL10"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
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</entry>
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        </submittedName>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4 5">RF1</strain>
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        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF96241.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
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        </recommendedName>
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        <name evidence="9" type="ORF">DMAD_05098</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
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        </reaction>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DEUBIQUITINASE OTUD6B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
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        </location>
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        <location>
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        <location>
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            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
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        </location>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="313" mass="35642" checksum="0CC38F344FBD97AB" modified="2024-11-27" version="1">MAKGGLEIFKMEPKLKEIHLEDVATRHRRERKELQAKLQAMKKNAPKNNKTKRKEFLDEMSRLENELEQRQKVELQADNSKDVSELSKNEHFAGVPTAACQPAKSKEDEEKRQEPGTQRVSKAQKRRDRKAREAREREEEIRVELQNAANDGPSIKLVELQQITAKLSGRQLALHSIASDGDCLYHAVLHQLNVNSLPGHSVQELRKETANYVRAQKDTFVSYMTHPKSGDLLNEEQFEQYCKDIDENHAWGGHIELKAMSSLLKVPIEVIQADGAPTLLGQEEFGGAPLVLCFHRHMYQLGAHYNSTVPVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL15</accession>
    <name>A0AAU9FL15_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">asparagine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Asparaginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_05218</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Asn) + L-asparagine + ATP = L-asparaginyl-tRNA(Asn) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9659"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="3"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FL15"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000040">
        <property type="entry name" value="Asparagine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF16">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="11"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF96626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="313" mass="36480" checksum="88B0D8A92C172F56" modified="2024-11-27" version="1">MIRGENTSKVLKMRSIVMQAFRDHYFEQGYNEVTPPTLVQTQVEGGSTLFKMQFFGEEAFLTQSSQLYLETCLPALGDVFTIAQSYRAEQSRTRRHLAEYTHVEAECPFISYDDLLDRLEDLVCDVVDRVLKSPFGHLVMELNPDFKAPKKPFRRMDYAAAIDWLKSNNITKDDGTFYEFGEDIPEAPERKMTDTINEPILLCRFPVAIKSFYMSRCPEDQRLTESVDVLLPNVGEIIGGSMRIWDSEELLKGYEREGIDPTPYYWYTDQRVYGTMPHGGYGLGLERFLCWLLNRYHIREVCLYPRFLDRCRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL19</accession>
    <name>A0AAU9FL19_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Misexpression suppressor of ras 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_13541</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FL19"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000362">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039142">
        <property type="entry name" value="NRF1/Ewg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20338">
        <property type="entry name" value="NUCLEAR RESPIRATORY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF96318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="31313" checksum="B7A1DE3840A17AF3" modified="2024-11-27" version="1">MPHGRAPETRRRLGSVSDSAPPSESSDGTGFSSASSEQRDELILHADWSGAHSHSSAQRSSAHGTTSLYNASDIDADTISTDTTSNTNLSDYERGQVESFFGGLGTEIFVSGALANLYEGTGNDGDWRLVFTGIPVILHDKGNSRARSMPRVTLVLAERGSCFALWSDRIDNLSNYRIAGPSFHTMCLSSNHQQMIGFSFDSTDAARELWQHVERLVSDPENIALTVPGTRKQKKQKRAKAAPLPPKSQISHPCQFHHVTSVVKEDSERYYSMQAFGPHNPQQHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL24</accession>
    <name>A0AAU9FL24_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycine--tRNA ligase</fullName>
            <ecNumber evidence="5">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Glycyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_05129</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2 ATP + H(+) = P(1),P(4)-bis(5'-adenosyl) tetraphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34935"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58141"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:34936"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>tRNA(Gly) + glycine + ATP = glycyl-tRNA(Gly) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:16014"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="5"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FL24"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070150">
        <property type="term" value="P:mitochondrial glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00858">
        <property type="entry name" value="GlyRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00935">
        <property type="entry name" value="GlyRS_RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.230:FF:000001">
        <property type="entry name" value="Glycine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.200:FF:000001">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
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    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
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            <begin position="9"/>
            <end position="65"/>
        </location>
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            <begin position="66"/>
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        </location>
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        <source>
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        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <sequence length="679" mass="75876" checksum="8EB9154265C52F99" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL29</accession>
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        <name evidence="2" type="ORF">DMAD_13581</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
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        <location>
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        </location>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF96367.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL34</accession>
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        <submittedName>
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        <name type="scientific">Drosophila madeirensis</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="31"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF96499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="148" mass="16754" checksum="0B7F4FC1C0B59F54" modified="2024-11-27" version="1">MYALLEHSFQCREGDDQRCYFTLPPLVAPLKCSILPLSNNAEFQPFTQKLSSFLTKAELSHKVDDSSGSIGRRYARTDEIAIPYGITVDFDTLKEPHTVTLRDRNSMKQVRVGLEKVVGVVKDLATARTSWEQVVAQYPVFEQQEATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL40</accession>
    <name>A0AAU9FL40_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_13583</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the POU transcription factor family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF96370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FL40"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:regulation of antimicrobial peptide biosynthetic process"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN NUBBIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00389">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
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            <begin position="428"/>
            <end position="502"/>
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        <location>
            <begin position="528"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="530"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="101"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="315"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="588"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
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            <begin position="132"/>
            <end position="150"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000682"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF96370.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="609" mass="66249" checksum="2E08A4FE8BA5B095" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL64</accession>
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        <submittedName>
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        </submittedName>
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        <name evidence="9" type="ORF">DMAD_05131</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9 10">RF1</strain>
        </source>
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        <subcellularLocation>
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        </subcellularLocation>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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        </location>
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        </location>
    </feature>
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            <end position="48"/>
        </location>
    </feature>
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    <feature type="compositionally biased region" description="Polar residues" evidence="7">
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF10495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF96503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2810" mass="317649" checksum="E75B89E68AF6714E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL76</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">DMAD_05270</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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            <dbReference type="ChEBI" id="CHEBI:72745"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:36140"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + ATP + CoA = 12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52112"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:90718"/>
            <dbReference type="ChEBI" id="CHEBI:136408"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:52113"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + ATP + CoA = 15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52116"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:78832"/>
            <dbReference type="ChEBI" id="CHEBI:136409"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:52117"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = 5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65341"/>
            <dbReference type="ChEBI" id="CHEBI:136407"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:52109"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hexadecanoate + ATP + CoA = hexadecanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:30751"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:30752"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="13"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FL89"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047676">
        <property type="term" value="F:arachidonate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05927">
        <property type="entry name" value="LC-FACS_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045311">
        <property type="entry name" value="LC-FACS_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF107">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 13 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="14">
        <location>
            <begin position="77"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF96701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="654" mass="73009" checksum="C189D8D432AEF491" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTA4</accession>
    <name>A0AAU9FTA4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_07019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTA4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF99013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="127" mass="14630" checksum="47293D52A98870A6" modified="2024-11-27" version="1">MVEMLHREPAPPAAPRPPTRGTTSNSRRRQRRGSGKCIWPTTEWLRKLRANSRTLSPAGASEEQLQQQDHQQQRRSSVLLMVLLHSSSNDEMKAKAVLVSEEQQQRERQRQNLPQDEVEDAIDALWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTC3</accession>
    <name>A0AAU9FTC3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcription factor AP-2-epsilon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_06808</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTC3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004979">
        <property type="entry name" value="TF_AP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013854">
        <property type="entry name" value="TF_AP2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812:SF17">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03299">
        <property type="entry name" value="TF_AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01748">
        <property type="entry name" value="AP2TNSCPFCT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor AP-2 C-terminal" evidence="8">
        <location>
            <begin position="241"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="24"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="24"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF98731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="465" mass="51097" checksum="4F965148B4404F31" modified="2024-11-27" version="1">MHILHRTNEHQFEDQKFMMERLSGHGSLGLSSSSAAYTTHSGGHTGRSGPATGHSGHSGTHGSAATMHHQSLQSDFQPPYFPPPFHHSTQSPPQQQNHGALEYLGTDPYGQPLSSLHHAPLHHYNQLAGLRSTQDQLGIHRTHREAELQGHVSQLAHGFPYTDRRGDYSSAISAGAAHGTRLGHEHESLALHQALQNAVDDVQAPAIDDNVAFMSDLPLIKSMKSGKDAGNIGSGSPSEVFCAVPGRLSLLSSTSKYKVTIAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRLLREKLEKIGLNLPAGRRKAANVTLLTSLVEGEATHLAKDFHFVCETEFPARQLAEYIVRHQTEPNDSYRRKELILHSQQITKELMQILSQDRTTHFGTRSQHLLEPSMQRHLTHFSLITHGFGSPAIMAVLHAFQTFLNESLNYLEKLYPSNGGGMVSSSLDKSKMDNEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTD4</accession>
    <name>A0AAU9FTD4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcription factor AP-2-epsilon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_06808</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTD4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004979">
        <property type="entry name" value="TF_AP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013854">
        <property type="entry name" value="TF_AP2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812:SF17">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03299">
        <property type="entry name" value="TF_AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01748">
        <property type="entry name" value="AP2TNSCPFCT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor AP-2 C-terminal" evidence="8">
        <location>
            <begin position="235"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="22"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="22"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="81"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF98729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="459" mass="50065" checksum="F1F4A50A39C13D63" modified="2024-11-27" version="1">MSFLATLDASAWQERLSGHGSLGLSSSSAAYTTHSGGHTGRSGPATGHSGHSGTHGSAATMHHQSLQSDFQPPYFPPPFHHSTQSPPQQQNHGALEYLGTDPYGQPLSSLHHAPLHHYNQLAGLRSTQDQLGIHRTHREAELQGHVSQLAHGFPYTDRRGDYSSAISAGAAHGTRLGHEHESLALHQALQNAVDDVQAPAIDDNVAFMSDLPLIKSMKSGKDAGNIGSGSPSEVFCAVPGRLSLLSSTSKYKVTIAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRLLREKLEKIGLNLPAGRRKAANVTLLTSLVEGEATHLAKDFHFVCETEFPARQLAEYIVRHQTEPNDSYRRKELILHSQQITKELMQILSQDRTTHFGTRSQHLLEPSMQRHLTHFSLITHGFGSPAIMAVLHAFQTFLNESLNYLEKLYPSNGGGMVSSSLDKSKMDNEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTD6</accession>
    <name>A0AAU9FTD6_DROMD</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ORF">DMAD_06706</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTD6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097733">
        <property type="term" value="C:photoreceptor cell cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000094">
        <property type="entry name" value="protein C21orf2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 410-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849">
        <property type="entry name" value="LEUCINE RICH REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="157"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="299"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="225"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="353"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF98570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="428" mass="48833" checksum="97FAD5D2C798D0A3" modified="2024-11-27" version="1">MTRLTEEMVIARAKQSDLGLITKLNCWGSDLTDVSIIKRMRGVEVLALSVNKISTLAPFEDCSKLQELYLRKNHIQDINEIAYLQNLPALKNLWLEENPCCEQSGSNYRAIVLRALPNLKKLDNVEVTQEEVDDALRRGGGGGGGGSVPEDEVYEDAYEARQQQRTSPQHSYPTHSPPPQQQAQQQQQYHPQTQQQPQQQQQQQQQRQSSSPIMREEPVHPPSPMYNTLTKEQRTSYQQYDRSPGSDQESSPNAGYREVRPAPIPPSISAHSMKEYYQSDRPAYPAHYRHSQTDLTEWEEHQQHQVPQVHHNPYGSQMLNHPQRRSVGPESGERFTGYRNGSARENGGDWDPEERPRSSRRPEGRYSDSTNNVSASVANHYSGYHRRPINRSSNLLSATLCLVKELDYASLEVLEHAVRCRIDELATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTD8</accession>
    <name>A0AAU9FTD8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_06817</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278:SF32">
        <property type="entry name" value="NEUROMUSCULIN, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278">
        <property type="entry name" value="SIDESTEP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043358789" description="Ig-like domain-containing protein" evidence="9">
        <location>
            <begin position="44"/>
            <end position="2021"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="984"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="22"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="155"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="265"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="377"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="475"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="580"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="669"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="788"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="873"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1019"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1170"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1320"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1366"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1390"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1447"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1610"/>
            <end position="1629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1691"/>
            <end position="1714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1975"/>
            <end position="1996"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1820"/>
            <end position="1847"/>
        </location>
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        <location>
            <begin position="1047"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1078"/>
            <end position="1123"/>
        </location>
    </feature>
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        <location>
            <begin position="1170"/>
            <end position="1190"/>
        </location>
    </feature>
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        <location>
            <begin position="1375"/>
            <end position="1385"/>
        </location>
    </feature>
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        <location>
            <begin position="1394"/>
            <end position="1404"/>
        </location>
    </feature>
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        <location>
            <begin position="1409"/>
            <end position="1419"/>
        </location>
    </feature>
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        </location>
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        <location>
            <begin position="1469"/>
            <end position="1479"/>
        </location>
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        <location>
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            <end position="1701"/>
        </location>
    </feature>
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        <location>
            <begin position="1980"/>
            <end position="1996"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="BFF98746.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2021" mass="223533" checksum="9D18EBE24A806FB2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTD9</accession>
    <name>A0AAU9FTD9_DROMD</name>
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        <submittedName>
            <fullName evidence="12">Calcium-binding mitochondrial carrier F55A11.4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_06852</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
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    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="63"/>
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        </location>
    </feature>
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    </evidence>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="363" mass="40413" checksum="C54A3F25624E71DA" modified="2024-11-27" version="1">MLRNSTYHYNLPVDYHNIQQMAVAGDMMEDFVVIFRDMMGRYLDIGEDMNVPDDFTQKEMQTGLWWRHLVAGGIAGGVSRTCTAPLDRIKVYLQVQTTRMGISECAQIMLNEGGSRSMWRGNGINVLKIAPETALKFAAYEQMKRLIRGEDASRQMSIVERFYAGAAAGGISQTIIYPMEVLKTRLALRKTGQYAGIADAAAKIYKHEGARSFYRGYVPNILGILPYAGIDLAVYETLKRRYIASHDNNEQPSFLVLLACGSTSSALGQLCSYPLALVRTRLQAQAAETITNQKRKTLIPLKSSDAHSGQETMTGLFRKIVKQEGLTGLYRGITPNFLKVLPAVSISYVVYEYSSRALGIKMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTE1</accession>
    <name>A0AAU9FTE1_DROMD</name>
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        <submittedName>
            <fullName evidence="13">Protein nervous wreck</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07091</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTE1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of supramolecular fiber organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FCH and double SH3 domains protein"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000155">
        <property type="entry name" value="FCH and double SH3 domains protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF21">
        <property type="entry name" value="PROTEIN NERVOUS WRECK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="12">
        <location>
            <begin position="11"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="545"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="638"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="431"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="703"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="749"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="930"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="497"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="713"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="789"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="821"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="876"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
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            <end position="1015"/>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
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        </source>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="BFF99113.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1015" mass="111367" checksum="77684CC8C9C9FAB8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTF0</accession>
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        </submittedName>
    </protein>
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        <name evidence="7" type="ORF">DMAD_07054</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
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        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
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        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTF1</accession>
    <name>A0AAU9FTF1_DROMD</name>
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        <submittedName>
            <fullName evidence="7">Lipase member H</fullName>
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        <name evidence="7" type="ORF">DMAD_07274</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
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        <property type="entry name" value="Lipase"/>
    </dbReference>
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        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
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        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF173">
        <property type="entry name" value="LIPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
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    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="6">
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            <end position="319"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
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        </source>
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            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF99356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="375" mass="41722" checksum="AEF9EF7E07F20160" modified="2024-11-27" version="1">MATITSNTLKTLFVALIIPIMAIPQENKPKLPANIETIQKHNIQLLTEQLNRGWRAFCDAPVDEGVMSLFQGINPSDAALHVMTITNQSVQLPIKSISLLKDFDINPERKTLVYVNAFHTADSYFSVQEHLSLLQSSRRDLNVIVVDFARDVAQLYYAVRHHLSVHGYAIYNVLKALKDAGIAPQDITLAGHSVGANIAALGAQLFARGSKQKVGQLVAIDPATMCRTTDILVKQSVAERVVVLHGEGDVFGVRVPVGHIDIYPNGIGYFPRRKLQPGCDSKICSHMYPFVLFMEALIEGVMIPATKCESWAKFRQSDCQFQNTINIGLIYPSTARGLYFCLTQPNPPFTYMEHGLRYKTTPRTKPEKALKKKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTF9</accession>
    <name>A0AAU9FTF9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neurabin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Neurabin-I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Neural tissue-specific F-actin-binding protein I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Protein phosphatase 1 regulatory subunit 9A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_07260</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06790">
        <property type="entry name" value="PDZ_neurabin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09512">
        <property type="entry name" value="SAM_Neurabin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000010">
        <property type="entry name" value="Neurabin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000008">
        <property type="entry name" value="Neurabin-1 isoform 1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
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        <location>
            <begin position="494"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
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        <location>
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        <location>
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        <location>
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        <location>
            <begin position="349"/>
            <end position="359"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="952" mass="103099" checksum="B651ABF03065F1F7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTG5</accession>
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        <submittedName>
            <fullName evidence="9">Probable E3 ubiquitin-protein ligase HERC4</fullName>
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        <name evidence="9" type="ORF">DMAD_06728</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9 10">RF1</strain>
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        <subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="HECT AND RLD DOMAIN CONTAINING E3 UBIQUITIN LIGASE 4, ISOFORM C"/>
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        <location>
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        <location>
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        <location>
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        <location>
            <position position="1022"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF98600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1054" mass="118483" checksum="25CBD0FB3A578C49" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTG6</accession>
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        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="11" type="ORF">DMAD_06842</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
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    </dbReference>
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    <dbReference type="SUPFAM" id="SSF57667">
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="369"/>
            <end position="396"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="397"/>
            <end position="424"/>
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    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="509"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="537"/>
            <end position="564"/>
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    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="565"/>
            <end position="592"/>
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                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG02961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="675" mass="74583" checksum="7145B7DD5D6936A1" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5B7</accession>
    <name>A0AAU9G5B7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">A disintegrin and metalloproteinase with thrombospondin motifs 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_02473</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ZnMc_salivary_gland_MPs"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sol narae, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1620.60:FF:000007">
        <property type="entry name" value="Sol narae, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADAMTS_CR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034030">
        <property type="entry name" value="ZnMc_salivary_gland_MPs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF249">
        <property type="entry name" value="SOL NARAE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17771">
        <property type="entry name" value="ADAMTS_CR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13582">
        <property type="entry name" value="Reprolysin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043717590" evidence="10">
        <location>
            <begin position="27"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="11">
        <location>
            <begin position="309"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
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            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="755"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="274"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="644"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="691"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="771"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="794"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="850"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="470"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG03148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="866" mass="96262" checksum="841D47ABEE4654D0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5B8</accession>
    <name>A0AAU9G5B8_DROMD</name>
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        <submittedName>
            <fullName evidence="15">Kinesin-like protein KIF13B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_02593</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22706">
        <property type="entry name" value="FHA_KIF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinesin family member 13A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinesin family member 13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000014">
        <property type="entry name" value="Uncharacterized protein, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
        <property type="entry name" value="Kinesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12473">
        <property type="entry name" value="DUF3694"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="5"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="14">
        <location>
            <begin position="1834"/>
            <end position="1876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="771"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="843"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1427"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1466"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1583"/>
            <end position="1606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1634"/>
            <end position="1712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1741"/>
            <end position="1770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1891"/>
            <end position="1925"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="589"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1466"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1476"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1634"/>
            <end position="1644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1678"/>
            <end position="1695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1701"/>
            <end position="1712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1750"/>
            <end position="1765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1898"/>
            <end position="1911"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="100"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG03304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1925" mass="215910" checksum="BDE5A41F7AA74C1A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5C4</accession>
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        <submittedName>
            <fullName evidence="13">Proton channel OtopLc</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_02176</name>
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    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the otopetrin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9G5C4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015252">
        <property type="term" value="F:proton channel activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Otopetrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522:SF62">
        <property type="entry name" value="OTOPETRIN-LIKE A, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522">
        <property type="entry name" value="PROTON CHANNEL OTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03189">
        <property type="entry name" value="Otopetrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="654"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="722"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="748"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="794"/>
            <end position="813"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="825"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="867"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="164"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="186"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG02750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="943" mass="103395" checksum="E535B3F54E3718F7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5C8</accession>
    <name>A0AAU9G5C8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Guanine nucleotide exchange factor DBS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_02453</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G5C8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051336">
        <property type="entry name" value="RhoGEF_Guanine_NuclExch_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056466">
        <property type="entry name" value="Spectrin_DBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826:SF211">
        <property type="entry name" value="LD43457P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826">
        <property type="entry name" value="RHO GUANINE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23289">
        <property type="entry name" value="Spectrin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DH_2"/>
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            <end position="1132"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
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            <strain evidence="2 3">RF1</strain>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG02892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="121" mass="12890" checksum="CE447D5D5B67E0C1" modified="2024-11-27" version="1">MKYLCVFVMLAMCLASSWAAISEPAGALPEIPEQDAAAAGAIGEKKTEKRGIYGFGHGYGGYGGYGGYGGYGHGYGDYGHGHYGYASPYYGSIGYVHSAPYYGGHHGGYYPYHGHGHYGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Drosophilidae</taxon>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </subcellularLocation>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 12" id="KW-0833">Ubl conjugation pathway</keyword>
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        <location>
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            <end position="1490"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
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        </location>
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        <location>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    </dbReference>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="501" mass="55994" checksum="634CE4AAE191385B" modified="2024-11-27" version="1">MANFDDTLQRARLAFASGKTRNVNFRRKQLENLLRCYEENENDIISALEADLRRPKQESLIVETEFMKNDIKHILYHLSDWVKAEKPSKSIINVMDDVQIYNDPFGVVLVIGAWNYPLQLLLVPVASAIAAGNCVVIKPSEIAGNCAKFIAEVIPKYLDNDCYPVVCGGPSETAELLKQRFDYIFYTGSTRVGKIIHAAANKHLTPTTLELGGKSPCYIDKSVELRTAVKRILWGKLINCGQTCIAPDYILCSKEMQDKFVAEAKDVLKEWYGENIQSSPDLSRVINTNNFQRLIGLMKSGRVAVGGKYDASERYIEPTILVDVKPNDPIMEEEIFGPILPIYNVESAYDAIKFINAREKPLVIYVFSNSNKLVKEFQSNTTSGGFCSNETIMHCGVDVLPFGGVGMSGMGSYHGKYGFDTFTHKKSCLGKDLSAVGEKLASARYPPYSDRKGSFLSFLLRKRRPLPNFYLTHVLAVGLGVGLTVLANYYLQVRKGKLLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5F6</accession>
    <name>A0AAU9G5F6_DROMD</name>
    <protein>
        <submittedName>
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        </submittedName>
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        <name evidence="13" type="ORF">DMAD_02176</name>
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    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
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        <lineage>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the otopetrin family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFG02754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G5F6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015252">
        <property type="term" value="F:proton channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004878">
        <property type="entry name" value="Otopetrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522:SF62">
        <property type="entry name" value="OTOPETRIN-LIKE A, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522">
        <property type="entry name" value="PROTON CHANNEL OTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03189">
        <property type="entry name" value="Otopetrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="667"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="735"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="761"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="807"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="838"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="880"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="177"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="485"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG02754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="952" mass="104356" checksum="76BB5901D0A63917" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5F9</accession>
    <name>A0AAU9G5F9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">A disintegrin and metalloproteinase with thrombospondin motifs 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_02473</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G5F9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ZnMc_salivary_gland_MPs"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sol narae, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1620.60:FF:000007">
        <property type="entry name" value="Sol narae, isoform D"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADAMTS_CR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034030">
        <property type="entry name" value="ZnMc_salivary_gland_MPs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF249">
        <property type="entry name" value="SOL NARAE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17771">
        <property type="entry name" value="ADAMTS_CR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13582">
        <property type="entry name" value="Reprolysin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043482369" evidence="10">
        <location>
            <begin position="21"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="11">
        <location>
            <begin position="303"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="253"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="639"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="268"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="639"/>
            <end position="649"/>
        </location>
    </feature>
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        <location>
            <position position="465"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG03150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="688" mass="77131" checksum="7D11B1FE75352F14" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5G1</accession>
    <name>A0AAU9G5G1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">DNA repair protein complementing XP-C cells homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_02505</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPC family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G5G1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000111">
        <property type="term" value="C:nucleotide-excision repair factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071942">
        <property type="term" value="C:XPC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.20.110:FF:000001">
        <property type="entry name" value="DNA repair protein complementing XP-C cells"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2460:FF:000001">
        <property type="entry name" value="DNA repair protein Rad4 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.20.110">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2460">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018327">
        <property type="entry name" value="BHD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004583">
        <property type="entry name" value="DNA_repair_Rad4"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cysteine proteinases"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="991"/>
            <end position="1043"/>
        </location>
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    <feature type="domain" description="Rad4 beta-hairpin" evidence="10">
        <location>
            <begin position="1045"/>
            <end position="1100"/>
        </location>
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    <feature type="domain" description="Rad4 beta-hairpin" evidence="11">
        <location>
            <begin position="1107"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="149"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="250"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="515"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="93"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="283"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="308"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
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        <source>
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        </source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01032"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03193.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="13">Proton channel OtopLc</fullName>
        </submittedName>
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        <name evidence="13" type="ORF">DMAD_02176</name>
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        <name type="scientific">Drosophila madeirensis</name>
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        <dbReference type="NCBI Taxonomy" id="30013"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13 14">RF1</strain>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="2">Belongs to the otopetrin family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
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            <end position="114"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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            <begin position="31"/>
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            <begin position="32"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG02756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="933" mass="102306" checksum="D4A74CCEBC17BC5C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5H1</accession>
    <name>A0AAU9G5H1_DROMD</name>
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        <recommendedName>
            <fullName evidence="13">beta-galactoside alpha-(2,6)-sialyltransferase</fullName>
            <ecNumber evidence="13">2.4.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_02669</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a beta-D-galactoside + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha-neuraminyl-(2-&gt;6)-beta-D-galactosyl derivative + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52104"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28034"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:136398"/>
            <dbReference type="EC" id="2.4.3.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
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    <dbReference type="EC" id="2.4.3.1" evidence="13"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G5H1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003835">
        <property type="term" value="F:beta-galactoside alpha-2,6-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097503">
        <property type="term" value="P:sialylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23968">
        <property type="entry name" value="GT29_ST6GAL1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000018">
        <property type="entry name" value="Sialyltransferase, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46059">
        <property type="entry name" value="BETA-GALACTOSIDE ALPHA-2,6-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46059:SF1">
        <property type="entry name" value="BETA-GALACTOSIDE ALPHA-2,6-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
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    <feature type="chain" id="PRO_5043627972" description="beta-galactoside alpha-(2,6)-sialyltransferase" evidence="15">
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            <begin position="30"/>
            <end position="447"/>
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            <begin position="36"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023034"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00034329"/>
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            <dbReference type="SAM" id="SignalP"/>
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            <dbReference type="EMBL" id="BFG03404.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
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    <sequence length="447" mass="51177" checksum="BF919E8D482A4273" modified="2024-11-27" version="1">MWKVRRALSGQCQTLLGVLLLSAALIIQSVQIGATKAQPRHEPAAAESNAAEPRVRGNERRLTRSVFHVRWNPNEKFVVESRESPAINSSKLAPHPRLKVSKNTKLTLSPKLYMCHDKYSDQCHNKTQQFRQRIVQTFEKSMSESLNDSHPNPYHVDYKPVFGDSFEEQYYPSTCLVMEAGVRVLRRKDAPFDKMPFGRLFPRQKLFRNIKNIKTCAIVSSAGSLAGSKLGRFIDTHDIVMRFNHAPTQGHEGDVGSKTTIRVVNSQVVTKPEFDFAHAPIFRNVTIAAWDPGKYNGTLEDWLTSSDYDLFTNYEIYRRRHPKSRAFLIDPHSVWRLWQTLQMFAGSRSIRRNPPSSGFIGLALLLPHCPQVDFVEYIPSTRLNGRCHYYSKEMNAACTFGSWHPLAAEKLMTLDMNTADDMSVFQFGILRIRRPDRLLCGFNFFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5H3</accession>
    <name>A0AAU9G5H3_DROMD</name>
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        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
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        <name evidence="7" type="ORF">DMAD_02486</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <location>
            <position position="162"/>
        </location>
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            <name>ATP</name>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG03165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1029" mass="116845" checksum="8FD81E4AA7C93648" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFB0</accession>
    <name>A0AAU9GFB0_DROMD</name>
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        <submittedName>
            <fullName evidence="15">Neuropeptide CCHamide-2 receptor</fullName>
        </submittedName>
    </protein>
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        <name evidence="15" type="ORF">DMAD_04949</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Neuropeptide CCHamide-1 receptor"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Bombsn_rcpt-like"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF24">
        <property type="entry name" value="NEUROPEPTIDE CCHAMIDE-2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BOMBESINR"/>
    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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            <end position="216"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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    <feature type="transmembrane region" description="Helical" evidence="13">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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            <end position="366"/>
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            <end position="363"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="434"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG06436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="488" mass="52954" checksum="5D978E8534122E3B" modified="2024-11-27" version="1">MYAALMDMSQSVAASLAYAPLDLSGVGAVNGQNGSGSGIGPGSGSGSAGNESLDSDAGSFLGQHNASGNGPYVPVLERPETYIVSVLYTLIFIVGVLGNGTLVIIFFRHRSMRNIPNTYILSLALADLLVILVCVPVATIVYTQDSWPFERNMCRITEFFKDISIGVSVFTLTALSGERYCAIVNPLRKLQTKPLTVFTAVIIWIMAILLGMPSFLVSDIEVYPMPTAAGNGTIKVCSPFRSKIYAKYMMAAKASIYYVVPLCIIAVLYIMMAKRLHMSARDMPGEQLSVQSRTQARARRHVARMVVAFVVVFFICFFPYYVFELWYHFYPTAEEDFDSFWHMVRIVGFCTSFLNSCVNPVALYCVSGVFRQHFNRYLCCMCVKRQPHLRQHSTATGMMENTSVMSMRRSTYVGVGSGSGGGNLRASLHMNNNQGGGGGGGGGAMRGGSFHRQDSMPLQHRNGGTAAAAGSGPTTGPAGRSTTISEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFB2</accession>
    <name>A0AAU9GFB2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">protein kinase C</fullName>
            <ecNumber evidence="7">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">DMAD_04829</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30 31">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="7"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11622">
        <property type="entry name" value="HR1_PKN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11623">
        <property type="entry name" value="HR1_PKN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11625">
        <property type="entry name" value="HR1_PKN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05589">
        <property type="entry name" value="STKc_PKN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000001">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000002">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000058">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000008">
        <property type="entry name" value="serine/threonine-protein kinase N isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000038">
        <property type="entry name" value="serine/threonine-protein kinase N2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.160">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037313">
        <property type="entry name" value="PKN_HR1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02185">
        <property type="entry name" value="HR1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17 23 25" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 30" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="19" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="REM-1" evidence="29">
        <location>
            <begin position="44"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="29">
        <location>
            <begin position="148"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="29">
        <location>
            <begin position="244"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="972"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="28">
        <location>
            <begin position="1232"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="574"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="845"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="918"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="396"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="583"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="845"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="918"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1001"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="BFG06290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1299" mass="143529" checksum="AB3B3EECADBAA934" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFB4</accession>
    <name>A0AAU9GFB4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14114">
        <property type="entry name" value="STKc_Twitchin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform C"/>
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        <property type="entry name" value="Bent, isoform J"/>
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        <property type="entry name" value="Bent, isoform J"/>
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        <property type="entry name" value="Bent, isoform J"/>
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        <property type="entry name" value="Myosin light chain kinase"/>
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        <property type="entry name" value="Myosin-binding protein C, slow type"/>
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        <property type="entry name" value="Protein CBR-UNC-22"/>
        <property type="match status" value="4"/>
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        <property type="entry name" value="Titin a"/>
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        <property type="entry name" value="Titin isoform E"/>
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        <property type="entry name" value="titin isoform X1"/>
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        <property type="match status" value="76"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
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        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
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        <property type="match status" value="40"/>
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        <property type="match status" value="26"/>
    </dbReference>
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        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="37"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
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    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0728">SH3 domain</keyword>
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        <location>
            <begin position="9"/>
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            <begin position="1791"/>
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            <begin position="1891"/>
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        <location>
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            <begin position="3583"/>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
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        <property type="protein sequence ID" value="BFG06720.1"/>
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        <property type="component" value="Chromosome dot"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:protein kinase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:regulation of developmental process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:regulation of multicellular organismal process"/>
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        <property type="term" value="P:sarcomere organization"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFD4</accession>
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            <fullName evidence="14">FXNA-like protease</fullName>
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        <name evidence="19" type="ORF">DMAD_04777</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="19 20">RF1</strain>
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        <subcellularLocation>
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        <text evidence="3">Belongs to the peptidase M28 family.</text>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF22248"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF22249"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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        <property type="match status" value="4"/>
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        <property type="match status" value="5"/>
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            <dbReference type="PROSITE" id="PS50835"/>
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        <source>
            <dbReference type="EMBL" id="BFG06326.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">DMAD_13674</name>
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    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        </location>
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        </location>
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        </location>
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        <location>
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        </location>
    </feature>
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        <source>
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        </source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG06778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:sensory neuron axon guidance"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Yamamoto D."/>
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</entry>
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    </evidence>
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</entry>
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    <accession>A0AAU9GFH8</accession>
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        <submittedName>
            <fullName evidence="10">PDZ and LIM domain protein Zasp</fullName>
        </submittedName>
    </protein>
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        <name evidence="10" type="ORF">DMAD_05019</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LIM1_Enigma_like_1"/>
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    <dbReference type="CDD" id="cd09461">
        <property type="entry name" value="LIM3_Enigma_like_1"/>
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    <dbReference type="CDD" id="cd09360">
        <property type="entry name" value="LIM_ALP_like"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd23068">
        <property type="entry name" value="PDZ_ZASP52-like"/>
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        <property type="entry name" value="Uncharacterized protein, isoform Z"/>
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    <dbReference type="FunFam" id="2.10.110.10:FF:000067">
        <property type="entry name" value="Uncharacterized protein, isoform Z"/>
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    <dbReference type="FunFam" id="2.10.110.10:FF:000069">
        <property type="entry name" value="Uncharacterized protein, isoform Z"/>
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        <property type="entry name" value="Cysteine Rich Protein"/>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR050604">
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        <property type="entry name" value="PDZ_sf"/>
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    <dbReference type="InterPro" id="IPR006643">
        <property type="entry name" value="Zasp-like_motif"/>
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    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
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    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
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        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP-RELATED"/>
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    <dbReference type="Pfam" id="PF00595">
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        <property type="entry name" value="ZM"/>
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    </dbReference>
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        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="8"/>
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            <end position="366"/>
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    <feature type="domain" description="LIM zinc-binding" evidence="8">
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            <end position="2452"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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                <person name="Yamamoto D."/>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
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                <person name="Yamamoto D."/>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EXW0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="513"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF91828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="558" mass="64071" checksum="766316E7A0EBB9B1" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXX1</accession>
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        <recommendedName>
            <fullName evidence="1">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">DMAD_08425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="130"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF89739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="415" mass="47491" checksum="506F2319DEA1680C" modified="2024-11-27" version="1">MAAIKVPEWINAELFEDVLKSTVPGYTKVKTFKADIGSAAGENYATIMLRVNIEVELEDGKSKDVSYMLKLPHQLELYQEMMKQNNIFDIERMMYSEVVPEMEALYKEVGVDVIFGARSYDFKGAKTDYVLLEDLGQKGFKNANRLEGLDQTHTERVLKKLSQWHAASAVRVATKGAYPEILNLGFLKEESRPMMTDMIKGMMARFLKVCVTYEGHEEYIEQIKNLIPVTVDEMYKMAKIDAQEFNVLNHGDFWSNNVMFQYDAFGKIKEVYLVDYQLPKYGTVAQDLLNFLLSSTKLEDKLSKFDYYIKFYHDNLIEHLKILKYTKPLPTLRSIHSSLLKHGVFGYSVVTGVMGAVLLDPSENASFENFVGNSEAGEAFQMQLYTNPRYRKHIQVILPWLLNRGALETSAPTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EY63</accession>
    <name>A0AAU9EY63_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_08507</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EY63"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF89844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="202" mass="21580" checksum="E19A1EBE1EFC7A07" modified="2024-11-27" version="1">MLENLRKYYANQQQHPPAHSVNSSSLSNSSSSNSSMSQQQLKLNLNLPKQQQHLVPRRNSSTSNASSTAENFDTFITLRNATANTSTDYLSKQQMQRRAQQQSVPLTSALNYYGHGPPTPTPSLTPAVAYNGKLTATAMLSQVNLSRAAATQQGTGTAATSASLLPHQHQQHARATAKPVTASGVVPGPGAGGESFILEYEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EY68</accession>
    <name>A0AAU9EY68_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sterile alpha motif domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_10033</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Interacts promiscuously (via SAM domain) with EPHA5, EPHA6, EPHA7, EPHA8, EPHB1, EPHB2, EPHB3 and EPHB4 (via SAM domain) (in vitro).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EY68"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd09527">
        <property type="entry name" value="SAM_Samd5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000055">
        <property type="entry name" value="Sterile alpha motif domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051725">
        <property type="entry name" value="SAM-SH3_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12301:SF10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12301">
        <property type="entry name" value="SAM-DOMAIN, SH3 AND NUCLEAR LOCALIZATION SIGNALS PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SAM" evidence="9">
        <location>
            <begin position="7"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="669"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="9">
        <location>
            <begin position="767"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="182"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="601"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="635"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="734"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="850"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="918"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="607"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="652"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="734"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="748"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="850"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
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    <sequence length="2241" mass="246244" checksum="5E925785739EA893" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EY84</accession>
    <name>A0AAU9EY84_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Aminopeptidase</fullName>
            <ecNumber evidence="22">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">DMAD_10046</name>
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    <organism evidence="27 28">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.</text>
            <dbReference type="EC" id="3.4.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20 22">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="20 22">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <topology evidence="3">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="22"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EY84"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016285">
        <property type="term" value="F:alanyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000016">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000008">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000016">
        <property type="entry name" value="Glutamyl aminopeptidase"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF253">
        <property type="entry name" value="AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="22 27" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="11 22" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 20 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 22" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7 22" id="KW-0645">Protease</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12 20 22" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043840766" description="Aminopeptidase" evidence="23">
        <location>
            <begin position="19"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="26">
        <location>
            <begin position="37"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="24">
        <location>
            <begin position="267"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="25">
        <location>
            <begin position="579"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="343"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="21">
        <location>
            <position position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
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        </source>
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            <dbReference type="PIRSR" id="PIRSR634016-4"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="BFF91848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="929" mass="107376" checksum="D4D6BECFECCDE6DA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EY88</accession>
    <name>A0AAU9EY88_DROMD</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Insecta</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
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    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
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            <end position="284"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF91247.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="284" mass="31156" checksum="1B8E339FE0B53BB6" modified="2024-11-27" version="1">MKAASTGTPRRDLLLFSVVINALSLGLRSCHAVPIVGGRIVSTVGETSQYPFMVSLQDVIVGNATVAPTYRHFCGGSLISDRWILSAAHCVWRKNINTISAFIGYESIENVDLLEAYGLESAEYIYFQPSNFRNDIALLYMRRRYRSALGQDLQFAQLPPQGMTPDRNESCRIIGYGATQHAGPCQTQLFEAEVRVIGNKECRDIIGHVWAPQNGANTVCAMGIGHQDSCQGDSGGPLICPYGGRDYIYGLVSHGLTCGIKGMPSVYTVTRPYHDWVQLLLSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F1D0</accession>
    <name>A0AAU9F1D0_DROMD</name>
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        <recommendedName>
            <fullName evidence="14">Signal transducer and activator of transcription</fullName>
        </recommendedName>
    </protein>
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        <name evidence="17" type="ORF">DMAD_09415</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="12">Forms a homodimer or a heterodimer with a related family member.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the transcription factor STAT family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF91034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09919">
        <property type="entry name" value="SH2_STAT_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14786">
        <property type="entry name" value="STAT_CCD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="STAT_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.630:FF:000003">
        <property type="entry name" value="Signal transducer and transcription activator 6"/>
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    </dbReference>
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        <property type="entry name" value="EF-hand"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.20">
        <property type="entry name" value="STAT transcription factor, all-alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.630">
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.532.10">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="STAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048988">
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    </dbReference>
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        <property type="entry name" value="STAT_N_sf"/>
    </dbReference>
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        <property type="entry name" value="STAT_TF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015988">
        <property type="entry name" value="STAT_TF_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013801">
        <property type="entry name" value="STAT_TF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012345">
        <property type="entry name" value="STAT_TF_DNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013799">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11801">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01017">
        <property type="entry name" value="STAT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02864">
        <property type="entry name" value="STAT_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02865">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21354">
        <property type="entry name" value="STAT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00964">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47655">
        <property type="entry name" value="STAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0010">Activator</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13 14" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 14" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SH2" evidence="16">
        <location>
            <begin position="584"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="728"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU046415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF91034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="771" mass="86576" checksum="171B37C354E869F7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F1G4</accession>
    <name>A0AAU9F1G4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Peripheral plasma membrane protein CASK</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_09697</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAGUK family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F1G4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019098">
        <property type="term" value="P:reproductive behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10831">
        <property type="entry name" value="PDZ_CASK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12081">
        <property type="entry name" value="SH3_CASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14094">
        <property type="entry name" value="STKc_CASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000146">
        <property type="entry name" value="MAGUK p55 subfamily member 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000051">
        <property type="entry name" value="Peripheral plasma membrane protein CASK isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000062">
        <property type="entry name" value="peripheral plasma membrane protein CASK isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000016">
        <property type="entry name" value="peripheral plasma membrane protein CASK isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035473">
        <property type="entry name" value="CASK_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 16" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="12"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="L27" evidence="21">
        <location>
            <begin position="343"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="L27" evidence="21">
        <location>
            <begin position="402"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="20">
        <location>
            <begin position="494"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="591"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="19">
        <location>
            <begin position="720"/>
            <end position="892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00071925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="BFF91404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="907" mass="101972" checksum="92DB8F3AA3FA315F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F2B8</accession>
    <name>A0AAU9F2B8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">BK channel</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Maxi K channel</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_10072</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. Slo sub-subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F2B8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000035">
        <property type="entry name" value="Calcium-activated potassium channel slowpoke"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000005">
        <property type="entry name" value="calcium-activated potassium channel subunit alpha-1 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000015">
        <property type="entry name" value="Calcium-activated potassium channel subunit alpha-1 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
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        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01449">
        <property type="entry name" value="BKCHANNELA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16 23" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="8" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="22">
        <location>
            <begin position="356"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="22">
        <location>
            <begin position="782"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="700"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1122"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="700"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00060897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BFF91884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1152" mass="127704" checksum="A6444094F8B215E4" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9T1</accession>
    <name>A0AAU9F9T1_DROMD</name>
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        <submittedName>
            <fullName evidence="6">DDB1- and CUL4-associated factor 10 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_10466</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat DCAF10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DCA10"/>
    </dbReference>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14588">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14588:SF2">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="87"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="129"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="305"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="402"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="323"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="410"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="434"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF92400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="601" mass="67270" checksum="4F771BE42AA1B7B7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9T7</accession>
    <name>A0AAU9F9T7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_10527</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F9T7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17086">
        <property type="entry name" value="RAWUL_RING1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16739">
        <property type="entry name" value="RING-HC_RING1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000219">
        <property type="entry name" value="E3 ubiquitin-protein ligase RING1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000100">
        <property type="entry name" value="E3 ubiquitin-protein ligase RING2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032443">
        <property type="entry name" value="RAWUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043540">
        <property type="entry name" value="RING1/RING2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042741">
        <property type="entry name" value="RING1_RING-HC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46076:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RING1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46076">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RING1 / RING 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16207">
        <property type="entry name" value="RAWUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
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            <begin position="233"/>
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    </feature>
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            <begin position="282"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
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            <dbReference type="PROSITE" id="PS50089"/>
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            <dbReference type="EMBL" id="BFF92469.1"/>
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            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
    <sequence length="408" mass="44669" checksum="EB217EF10E38E385" modified="2024-11-27" version="1">MTSLDPAPNKTWELSLYELQRKPQDIITDSTEIAVSPRSLHSELMCPICLDMLKKTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSKRSLRADPNFDLLISKIYPSREEYEAIQEKVMAKFNQTQSQQALVNSINEGIKLQSQNRPQRFRTKGSGGAGGGGGGGGGGNASNDSNSNTNSTDRDNRDPGQSATSVTSANTSTSTVAPVTTPAPTGASTSATRMQVEDGSNPPSVRSTPSPVPSNSSSSKPKRAMSVLTSERSEESESDSQMDCRTEGDSIDTEGEGNGELGINDEIELVFKPHPTEMSADNQLIRALKENCVRYIKTTANATVDHLSKYLAMRLTLDLGADLPEACRLLNFCIYVAPQPQQLVILNGNQTLHQVNDKFWKVNKPMEMYYSWKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAU9F9U4_DROMD</name>
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        <submittedName>
            <fullName evidence="5">Protein unc-80 homolog</fullName>
        </submittedName>
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        <name evidence="5" type="ORF">DMAD_10531</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5 6">RF1</strain>
        </source>
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    </dbReference>
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            <end position="256"/>
        </location>
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            <begin position="3244"/>
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        </source>
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            <dbReference type="Pfam" id="PF15778"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF19424"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF20262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF92478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="3301" mass="368006" checksum="1487BEA962010EC0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9U8</accession>
    <name>A0AAU9F9U8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_10527</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="EMBL" id="AP029263">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="RAWUL_RING1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E3 ubiquitin-protein ligase RING1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E3 ubiquitin-protein ligase RING2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="Znf_RING_CS"/>
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        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RING1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46076">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RING1 / RING 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16207">
        <property type="entry name" value="RAWUL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF93056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="546" mass="61201" checksum="38945A64DE5FE4B5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBF5</accession>
    <name>A0AAU9FBF5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MADF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_10964</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBF5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF93031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="20139" checksum="77AFA2D2AB6642CC" modified="2024-11-27" version="1">MNKDVFSCKNAWKSLRDSYRYHMKMVAKGKAAVNNEDADWDPLADAKTRDNISGRWRFAPHMEFLYQSVQNRPVSTVFVGKVVKPKASPSPHGMSSPPAIPSPQARIPPQTKTASQARPAPQTRPICAYWESELNKLAPEDAETVEQKMTRLLWSETQALRLNAYLKEEDQDMYSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBG2</accession>
    <name>A0AAU9FBG2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Endoplasmic reticulum aminopeptidase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_10978</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBG2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000001">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000017">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000007">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF299">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="18">
        <location>
            <begin position="107"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="16">
        <location>
            <begin position="428"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="17">
        <location>
            <begin position="747"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFF93048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1090" mass="122596" checksum="DAEF23F1701EC82C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBH3</accession>
    <name>A0AAU9FBH3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">procollagen-proline 4-dioxygenase</fullName>
            <ecNumber evidence="5">1.14.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_10994</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the P4HA family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.2" evidence="5"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBH3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000011">
        <property type="entry name" value="Prolyl alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059068">
        <property type="entry name" value="TPR_P4H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF244">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23558">
        <property type="entry name" value="TPR_P4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0847">Vitamin C</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043998133" description="procollagen-proline 4-dioxygenase" evidence="14">
        <location>
            <begin position="24"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="15">
        <location>
            <begin position="406"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF93071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="538" mass="61469" checksum="AB82753369711ACA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBH4</accession>
    <name>A0AAU9FBH4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbonic anhydrase</fullName>
            <ecNumber evidence="4">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_10955</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="4"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBH4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00326">
        <property type="entry name" value="alpha_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF137">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="5">
        <location>
            <begin position="20"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF93020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="275" mass="31693" checksum="B41FA7873A98CC54" modified="2024-11-27" version="1">MFHVVRYFAKVSELSSPNPASFNYDKQGKDWQVQPGKQQSPIALVQSEALRYEGPQLVFFNYTKPLYNPYVTNNGHTIRMDISATIDKQNPALAGCNLETVYLAQQLHFHWGSERRQGSEHTIDSQRYDGELHIVHKNISYESNQEAVHHPDGFVVLAVMLRCSEVPQVQSQAVNEICKEVKLLPKCYDSSLLKAEISMEDLFAGIDMDKFFTYKGSLTSPPCWETVDWFVFPDALDIPKEINFWQLKDRRGKRLINTYRHLQASNSRPVYLCKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBI1</accession>
    <name>A0AAU9FBI1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nicastrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_10957</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nicastrin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBI1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007220">
        <property type="term" value="P:Notch receptor processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03881">
        <property type="entry name" value="M28_Nicastrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000127">
        <property type="entry name" value="Nicastrin, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041084">
        <property type="entry name" value="Ncstrn_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008710">
        <property type="entry name" value="Nicastrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21092">
        <property type="entry name" value="NICASTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21092:SF0">
        <property type="entry name" value="NICASTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18266">
        <property type="entry name" value="Ncstrn_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05450">
        <property type="entry name" value="Nicastrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043684092" description="Nicastrin" evidence="11">
        <location>
            <begin position="25"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="659"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="Nicastrin small lobe" evidence="12">
        <location>
            <begin position="40"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF18266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF93023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="699" mass="77978" checksum="17B8D4C8D1CBA579" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBI4</accession>
    <name>A0AAU9FBI4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Endoplasmic reticulum aminopeptidase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_10978</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBI4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000001">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000017">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000007">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF299">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="18">
        <location>
            <begin position="128"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="16">
        <location>
            <begin position="449"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="17">
        <location>
            <begin position="768"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="26"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFF93047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1111" mass="125012" checksum="10DFA95F3B0EC38D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCC8</accession>
    <name>A0AAU9FCC8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">tRNA-dihydrouridine(20a/20b) synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_11198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FCC8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF31">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20A_20B) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="1">
        <location>
            <begin position="2"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF93330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="195" mass="21624" checksum="F30C2AE2DDD001F8" modified="2024-11-27" version="1">MEEVRKSLQIPLIVNGNVESWSDACELHAQTGAAGVMAARGLLANPALFNSDYPTATTTPLACVEQWLDIAAAAGDNLLFQCFHHHLTFMWSSEMKRSLRVQFNSLSTKKQVVEFLGEHYNVRTSGPAPEPAPYTDCTYTHFTPPRHARHIAAEAEELAWSSRSDGKFFTEFKEHQISKPGGDDLELGDLFGAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCC9</accession>
    <name>A0AAU9FCC9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cylicin-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_11186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FCC9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="562"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="610"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF93312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="694" mass="74940" checksum="ABC91FF0A8978C1A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJV6</accession>
    <name>A0AAU9FJV6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DMAD_13272</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJV6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004245">
        <property type="entry name" value="DUF229"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974">
        <property type="entry name" value="FI08016P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974:SF1">
        <property type="entry name" value="FI08016P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02995">
        <property type="entry name" value="DUF229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF95979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="179" mass="20192" checksum="C80B91D5142DA0E3" modified="2024-11-27" version="1">MYETMKDLLHLDSLHGNRLKQKSSLLWKLRGSDTPRGVSLFLPVPSWRTCNTSNIPVAFCLCQKLTPLQLDDPTVALVARSALLSINQLLEPFPMCLQLELKAVVSAFSSVPHVTHKHDKRDFTVRFQTIPGDAYFEATSRLTDGVLHQAGEIIRIMHRNNNSNCISELQLEPFCYCAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJW0</accession>
    <name>A0AAU9FJW0_DROMD</name>
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        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_13166</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:neuronal cell body"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
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            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF95851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="450" mass="51781" checksum="75C9D30B3A2BD19E" modified="2024-11-27" version="1">MPTQKTRLHPYPRRISPYRTPSVNRNAFSHEARPPPPPPPLPKTLEHPVFEDIRTIMSVLKASGLMPIYEQLSSYKVGPPTKTNEFYSFFVRGVVHALTIFNVYSLFTPSSAQLFYSYRETDNVNQWIELLLCILTYTLTVFVCARNTKNVLRVMNEILQLDEEVRRRFGANLSQNFSFSVKYLVGITACQTYIIVLKIYAVEGVITPTSYVLLAFYAVQNGLTATYIVFASALLRIVYIRFHFINQLLNGYTYVQQQRRKGGNRRTTAAPSLMEHFPEDSLFIYRMHNKLLRIYKGINDCCNLILVSFLGYSFYTVTTNCYNLFVQITAKGMVSSNILQWCFAWLCMHVSLLALLSRSCGLTTREANATSQILARVYAKSKEYQNIIDKFLTKSIKQEVQFTAYGFFAIDNSTLFKIFSAVTTYLVILIQFKQLEDSKVEDHVQEQPQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJW2</accession>
    <name>A0AAU9FJW2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein Aster-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_13346</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF4">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF02893">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="1131"/>
        </location>
    </feature>
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        <location>
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        </location>
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        </location>
    </feature>
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    </feature>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
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            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
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            <end position="1079"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
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        <source>
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</entry>
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        <property type="entry name" value="Ski_Sno_DHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="290"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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            <begin position="132"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="400"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="576"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="939"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="989"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="829"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="148"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="175"/>
            <end position="195"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="203"/>
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        <location>
            <begin position="241"/>
            <end position="250"/>
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        <location>
            <begin position="584"/>
            <end position="608"/>
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    </feature>
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        <location>
            <begin position="639"/>
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        <location>
            <begin position="650"/>
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        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="995"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1021"/>
            <end position="1034"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF95943.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1132" mass="120124" checksum="7F423CADE846E94E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJY1</accession>
    <name>A0AAU9FJY1_DROMD</name>
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        <submittedName>
            <fullName evidence="22">Transient receptor potential-gamma protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_13252</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell projection</location>
            <location evidence="16">Rhabdomere membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJY1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
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    <dbReference type="GO" id="GO:0034703">
        <property type="term" value="C:cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0033583">
        <property type="term" value="C:rhabdomere membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015279">
        <property type="term" value="F:store-operated calcium channel activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Transient receptor potential-gamma protein"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
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    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
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    <dbReference type="InterPro" id="IPR013555">
        <property type="entry name" value="TRP_dom"/>
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    <dbReference type="InterPro" id="IPR002153">
        <property type="entry name" value="TRPC_channel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00870">
        <property type="entry name" value="trp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117:SF54">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL-GAMMA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08344">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRNSRECEPTRP"/>
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    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
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    <dbReference type="SMART" id="SM01420">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <keyword evidence="15" id="KW-0844">Vision</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="533"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="627"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="18">
        <location>
            <begin position="131"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Transient receptor ion channel" evidence="21">
        <location>
            <begin position="166"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="863"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="970"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1064"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="866"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="976"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1086"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="BFF95955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1128" mass="127006" checksum="66109A58C50E9BBA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FK70</accession>
    <name>A0AAU9FK70_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Lysosomal dipeptide transporter MFSD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Major facilitator superfamily domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="ORF">DMAD_13314</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28 29">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="23">Lysosomal dipeptide uniporter that selectively exports lysine, arginine or histidine-containing dipeptides with a net positive charge from the lysosome lumen into the cytosol. Could play a role in a specific type of protein O-glycosylation indirectly regulating macrophages migration and tissue invasion. Also essential for liver homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-alanyl-L-lysine(out) = L-alanyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79415"/>
            <dbReference type="ChEBI" id="CHEBI:192470"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-alpha-aminoacyl-L-arginine(out) = L-alpha-aminoacyl-L-arginine(in)</text>
            <dbReference type="Rhea" id="RHEA:79367"/>
            <dbReference type="ChEBI" id="CHEBI:229968"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-alpha-aminoacyl-L-histidine(out) = L-alpha-aminoacyl-L-histidine(in)</text>
            <dbReference type="Rhea" id="RHEA:79375"/>
            <dbReference type="ChEBI" id="CHEBI:229967"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-alpha-aminoacyl-L-lysine(out) = L-alpha-aminoacyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79383"/>
            <dbReference type="ChEBI" id="CHEBI:229966"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-arginyl-L-alpha-amino acid(out) = L-arginyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79371"/>
            <dbReference type="ChEBI" id="CHEBI:84315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-arginyl-glycine(out) = L-arginyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79391"/>
            <dbReference type="ChEBI" id="CHEBI:229955"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-aspartyl-L-lysine(out) = L-aspartyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79411"/>
            <dbReference type="ChEBI" id="CHEBI:229953"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-histidyl-L-alpha-amino acid(out) = L-histidyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79379"/>
            <dbReference type="ChEBI" id="CHEBI:229964"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-histidyl-glycine(out) = L-histidyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79395"/>
            <dbReference type="ChEBI" id="CHEBI:229957"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-lysyl-L-alanine(out) = L-lysyl-L-alanine(in)</text>
            <dbReference type="Rhea" id="RHEA:79399"/>
            <dbReference type="ChEBI" id="CHEBI:229954"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-lysyl-L-alpha-amino acid(out) = L-lysyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79387"/>
            <dbReference type="ChEBI" id="CHEBI:229965"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-lysyl-L-lysine(out) = L-lysyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79403"/>
            <dbReference type="ChEBI" id="CHEBI:229956"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-lysyl-glycine(out) = L-lysyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79407"/>
            <dbReference type="ChEBI" id="CHEBI:191202"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="24">Homodimer. Interacts with lysosomal protein GLMP (via lumenal domain); the interaction starts while both proteins are still in the endoplasmic reticulum and is required for stabilization of MFSD1 in lysosomes but has no direct effect on its targeting to lysosomes or transporter activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FK70"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17340">
        <property type="entry name" value="MFS_MFSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052187">
        <property type="entry name" value="MFSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="317"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="399"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="27">
        <location>
            <begin position="53"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00044924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00044985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00045709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00046376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EMBL" id="BFF96037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="482" mass="53454" checksum="706259AF7F06940A" modified="2024-11-27" version="1">MTREERQRIVDTSNSSSSGEDEAKPVVRRRSTRDTELAPPKDPGCCDPSSTPHRFLALVFMCLLGFGSYFCYDNPGALQDVFQKELDLNSTEFTLIYSIYSWPNIVLCFVGGFLIDRVFGIRLGTIIYMLIVLVGQLIFATGGVLGHFWLMIVGRFVFGIGAESLAVAQNSYAVLWFKGKELNMVFGLQLSVARFGSTVNFWIMQPLYGYVSKSYAGYTGLGVALFLASSTCVMSLICTLILGWMDKRAERILKRNNNPGGEIAKLSDIITFKMDFWMVSVVCVAYYVAIFPFVALGQAFFVNNFHMTPAEANTVNSIVYLISAIASPLFGFIIDKVGRNLTWVFCATISTFVAHALLTFTHLDPYIGMSIMGLSYSMLAASLWPLVSLIIPEYQLGTAYGFCQSVQNLGLAVVTIVAGIIVDSSGGSHFWLQLFFMLFLLISLLATVAIWAYNRKHQGNLNMSPSQRATYHTSMYVNIESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FK76</accession>
    <name>A0AAU9FK76_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_13322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FK76"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="114"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF96045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="280" mass="33137" checksum="D6EE6E7E8B5E754E" modified="2024-11-27" version="1">MEQGSGMQVDVCPNPMLQPQTSEWVVDPYRKRSESLKIFVAALVRSWRRRGENICQLEEETQHFRDEIKNSVRLSKALKDVELERNGELHLELMMYKRSFYEAQTSIYSLTTDKKKLIHEVECSKREHDELQAVADQRQIEICTALKKQLIDQQRLSQEEKAINRLRRKNADLVTEVTTLRRMQMEYKISEQCLTKELEETNERMSTIQEELNCLQDRMRIWNSNEPLEHEPQVRPKMEQQELFISNDSLQTECSKSNKAVFPMPQHVLLNALILFIKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FK77</accession>
    <name>A0AAU9FK77_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SKI/SNO/DAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_13245</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DACH/dachshund family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FK77"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21081">
        <property type="entry name" value="DHD_Dac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.20:FF:000001">
        <property type="entry name" value="Dachshund homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.20">
        <property type="entry name" value="Ski"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052417">
        <property type="entry name" value="Dachshund_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003380">
        <property type="entry name" value="SKI/SNO/DAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037000">
        <property type="entry name" value="Ski_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12577">
        <property type="entry name" value="DACHSHUND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12577:SF6">
        <property type="entry name" value="DACHSHUND, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02437">
        <property type="entry name" value="Ski_Sno_DHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SKI/SNO/DAC" evidence="6">
        <location>
            <begin position="187"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="297"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="473"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="836"/>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="4"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FK95"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000002">
        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="12">
        <location>
            <begin position="70"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="11">
        <location>
            <begin position="209"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF95960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="500" mass="53550" checksum="AC3AE48334278A25" modified="2024-11-27" version="1">MASLLALRGALRCWPHSRGAARIGSEAASTVVFTSTAFISKAASNAGSSIESLSSGFKFLKFKSSCNMTIKAIKARQIYDSRGNPTVEVDLTTENGLFRAAVPSGASTGVHEALELRDNDKANYHGKSVLKAVAHVNDTLGPEVIKANLDVVDQAAVDNFMIKLDGTENKSKFGANAILGISLAVAKAGAAKKNVPLYKHIADLAGNKEIILPVPAFNVINGGSHAGNKLAMQEFMILPTGATTFTEAMKMGSEVYHHLKNVIKAKFGLDATAVGDEGGFAPNIQSNKEALVLITDAIAKAGYTGKIEIGMDVAASEFFKDGLYDLDFKNPASDKAQWLTPDKLANLYQEFIKEFPITSIEDPFDQDHWDAWTNLTSNTKIQIVGDDLTVTNPKRIATAVEKGACNCLLLKVNQIGTVTESIAAHLLAKKNGWGTMVSHRSGETEDSFIGDLVVGLSTGQIKTGAPCRSERLAKYNQILRIEEEIGSGVKFAGKSFRKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FK97</accession>
    <name>A0AAU9FK97_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Neural cell adhesion molecule 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_13286</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FK97"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008046">
        <property type="term" value="F:axon guidance receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050958">
        <property type="entry name" value="Cell_Adh-Cytoskel_Orgn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45080">
        <property type="entry name" value="CONTACTIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45080:SF27">
        <property type="entry name" value="NEURAL CELL ADHESION MOLECULE 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043325347" evidence="3">
        <location>
            <begin position="21"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="36"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="150"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="234"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="330"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF95999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="455" mass="51552" checksum="91BC25607FB875B7" modified="2024-11-27" version="1">MDTFFAVCSLLLLLLSQNAAILGQLDGNTSPASSAPPLRLQPSTPSITHFVNDSFIIFCQTVQKDIDTKWRDPRGQTRENTKGRVHIEKKTGLLALVFEHISLDDRGNWTCEVTGQQNRNVNTEREFFSSFELLVNQKISFGKTEQVQSVREGRDAMVNCFVEGMPAPEVSWLYNGEYINTVNSSKHNRLSNGLYIRNVSQADAGEYTCRAMRITPTFSDSDQITILLRIQHKPHWFYNETLPMQYAYVGGAVNLSCDAMGEPPPSFTWLHNGKGILGFNHRIFVADYGATLQLNVRNDSQFGDYKCKVANPLGSLERVIKLRPGAKPMGPTRFQLKRLYADAFELDLRTPRMTNVSDEMQIFGYRVAYMSENEFKFSAGNWSHAKQRDFSFHRGQRFIISHLEGNTTYLMRAASRNLAGLSDWSGVKIFATAGGRRWYPSLYPLYALILALIYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKT0</accession>
    <name>A0AAU9FKT0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_13480</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000333">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000344">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PDZ domain-like"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1655"/>
            <end position="1733"/>
        </location>
    </feature>
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            <begin position="1746"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="732"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1038"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1101"/>
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        </location>
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            <begin position="1176"/>
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        </location>
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        <location>
            <begin position="1362"/>
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            <begin position="1522"/>
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        </location>
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        <location>
            <begin position="571"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        </source>
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        <source>
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        </source>
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            <dbReference type="EMBL" id="BFF96240.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1976" mass="220329" checksum="70DD239C539FC2E9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKT3</accession>
    <name>A0AAU9FKT3_DROMD</name>
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            <fullName evidence="5">Defective in cullin neddylation protein</fullName>
        </recommendedName>
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        <name evidence="8" type="ORF">DMAD_05083</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
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        <text evidence="5">Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Promotes neddylation of cullin components of SCF-type E3 ubiquitin ligase complexes and thus regulates SCF-type complex activity. Function promotes cell proliferation.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="RP42 RELATED"/>
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        </source>
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        </source>
    </evidence>
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        </source>
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            <dbReference type="EMBL" id="BFF96422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="243" mass="28518" checksum="88EE87A6007E3254" modified="2024-11-27" version="1">MDLASDNYFQNPEYYYRELDRKRIDQLYIRYRDPSDPLKISSQGVIRFLEDLDLKPDSKLVLIIAWKFHAEVQCEFSRDEFTNGMCELGIDSIEKLKSKLPLLELELNDTGKFKDFYHFTFNYAKDPGQKSIDLDMAIAYWCIVLSGRFKFLDIWCKFLEEKHKRAISRDTWNLLLDFATNIDDGMSNYDSEGAWPVLIDDFVEWCQENNHLKEDPSSGCQSTSQRNISSVYPTSHSTNMNYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKT6</accession>
    <name>A0AAU9FKT6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Epidermal growth factor receptor kinase substrate 8-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_13440</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <text evidence="2">Belongs to the EPS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3_Eps8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Epidermal growth factor receptor kinase substrate 8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Epidermal growth factor receptor kinase substrate 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039801">
        <property type="entry name" value="EPS8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055093">
        <property type="entry name" value="EPS8_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033928">
        <property type="entry name" value="EPS8_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035462">
        <property type="entry name" value="Eps8_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013625">
        <property type="entry name" value="PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
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        <property type="entry name" value="AROUSER, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12287">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR KINASE SUBSTRATE EPS8-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22975">
        <property type="entry name" value="EPS8_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08416">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="675"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="181"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="506"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="547"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="739"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="216"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="278"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="509"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="547"/>
            <end position="571"/>
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            <begin position="577"/>
            <end position="586"/>
        </location>
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    <feature type="compositionally biased region" description="Gly residues" evidence="7">
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            <end position="605"/>
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            <begin position="739"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF96194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="772" mass="84875" checksum="9A12D5EFA7785C4A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL52</accession>
    <name>A0AAU9FL52_DROMD</name>
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        <submittedName>
            <fullName evidence="7">Centrosomal protein of 135 kDa</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05124</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CEP135/TSGA10 family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF96488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FL52"/>
    <dbReference type="Proteomes" id="UP001500889">
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    </dbReference>
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        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR051877">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NUCLEOPROTEIN TPR_MLP1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="485"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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        </location>
    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="493"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF96488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1064" mass="123614" checksum="871C68A42F2AF09A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL57</accession>
    <name>A0AAU9FL57_DROMD</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">DMAD_13520</name>
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    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BFF96286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF96286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="89" mass="9521" checksum="46655FC1C755BC06" modified="2024-11-27" version="1">MPSLGGICSKACIAINVTSYVAASPKTSTDSMVLRTAIWSRRIPNHLGATYSILAPPQVKKSASHHYEAASKQPGTLMDCRRHGLDAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL59</accession>
    <name>A0AAU9FL59_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_05259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF96671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="266" mass="29103" checksum="0DF73442196B3B62" modified="2024-11-27" version="1">MLRHYPTATPIHFRIQLLSDKKVVMVEAAGYETEIFLPQLLDGRINLKNIIRSRQPRQQQDQHQLQNFDSFADSRSIQSKVRQRPGQSKLTTATAGLAPPTFNGVVFAAGGSNVGNGPQFALKLISNGSVVLVESNGYESEIFLPHISCAYVSMKRVTASQLVQCSRLTSRQPTRPEKINPNPTSAMGHPKSNRVNLPSATRRCGIGESKKESALTLTAKDGGEAFVSSRGKRRKTHHQNTAKARAKQAGAGDQPVKVKTLIPGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSG6</accession>
    <name>A0AAU9FSG6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein phosphatase 1 regulatory subunit 12B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Myosin phosphatase-targeting subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_06462</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Regulates myosin phosphatase activity. Augments Ca(2+) sensitivity of the contractile apparatus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12B mediates binding to myosin. Isoform 3 and isoform 4 bind PPP1R12A, but not isoform 1 of PPP1R12B itself. Binds IL16.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NRARP family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FSG6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019208">
        <property type="term" value="F:phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21930">
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    <sequence length="2111" mass="224474" checksum="1C385C4AA53B5F70" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSH4</accession>
    <name>A0AAU9FSH4_DROMD</name>
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        <recommendedName>
            <fullName evidence="10">FACT complex subunit</fullName>
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        <name evidence="16" type="ORF">DMAD_06639</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="16 17">RF1</strain>
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        <text evidence="10">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
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        <text evidence="10">Component of the FACT complex.</text>
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        <subcellularLocation>
            <location evidence="10">Nucleus</location>
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            <location evidence="10">Chromosome</location>
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        <text evidence="1 10">Belongs to the peptidase M24 family. SPT16 subfamily.</text>
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            <dbReference type="RuleBase" id="RU367052"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01285"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF98478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1086" mass="124026" checksum="87CC0DEDA2F50ACD" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSI0</accession>
    <name>A0AAU9FSI0_DROMD</name>
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        <recommendedName>
            <fullName evidence="1">Dipeptidase</fullName>
            <ecNumber evidence="1">3.4.13.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_06470</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an L-aminoacyl-L-amino acid + H2O = 2 an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:48940"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
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            <dbReference type="ChEBI" id="CHEBI:77460"/>
            <dbReference type="EC" id="3.4.13.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Peptidase M19 family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF98270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070573">
        <property type="term" value="F:metallodipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01301">
        <property type="entry name" value="rDP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008257">
        <property type="entry name" value="Pept_M19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443:SF12">
        <property type="entry name" value="DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443">
        <property type="entry name" value="MICROSOMAL DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01244">
        <property type="entry name" value="Peptidase_M19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51365">
        <property type="entry name" value="RENAL_DIPEPTIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0224">Dipeptidase</keyword>
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    <keyword evidence="1" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
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            <end position="421"/>
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        <location>
            <begin position="232"/>
            <end position="266"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="232"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU341113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF98270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="803" mass="85000" checksum="41DB9A9638D4796D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSI4</accession>
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        <submittedName>
            <fullName evidence="8">Arginine-glutamic acid dipeptide repeats protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="8" type="ORF">DMAD_06738</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd11661">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000052">
        <property type="entry name" value="Arginine-glutamic acid dipeptide (RE) repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.50:FF:000004">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000949">
        <property type="entry name" value="ELM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13859">
        <property type="entry name" value="ATROPHIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13859:SF11">
        <property type="entry name" value="GRUNGE, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01189">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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        </location>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSI5</accession>
    <name>A0AAU9FSI5_DROMD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_06673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FSI5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="entry name" value="Designed four-helix bundle protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="32"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF98530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="125" mass="14758" checksum="B05BCD0FDA56C8CE" modified="2024-11-27" version="1">MSMNLSAKLDELQRGDRHLETTVALCEIRTQLQELTKSVESCQSEVSEVKRDMVAIKHELDTVQQVKEEIEELREYVDRLEEHTHRRKLRLLEQVCSLNYLPCCVIKYLNFISLSHVLQTELHHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSJ5</accession>
    <name>A0AAU9FSJ5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Arginine-glutamic acid dipeptide repeats protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_06738</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome J"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="SANT_MTA3_like"/>
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    </dbReference>
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        <property type="entry name" value="Arginine-glutamic acid dipeptide (RE) repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.50:FF:000004">
        <property type="entry name" value="arginine-glutamic acid dipeptide repeats protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000949">
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    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13859">
        <property type="entry name" value="ATROPHIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13859:SF11">
        <property type="entry name" value="GRUNGE, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
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    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ELM2"/>
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    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="7"/>
            <end position="118"/>
        </location>
    </feature>
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        <location>
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        </location>
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        </location>
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        </location>
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            <end position="785"/>
        </location>
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        </location>
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        </location>
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        </location>
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            <end position="1186"/>
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        </location>
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        </location>
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            <end position="1384"/>
        </location>
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        </location>
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        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF98634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2089" mass="223090" checksum="A0F313311B41ACC6" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSJ8</accession>
    <name>A0AAU9FSJ8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Acyl carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_06725</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
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        <text evidence="14">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
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        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002148">
        <property type="entry name" value="PRK00982.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="ACYL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863:SF28">
        <property type="entry name" value="ACYL CARRIER PROTEIN, MITOCHONDRIAL"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 14" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Carrier" evidence="15">
        <location>
            <begin position="76"/>
            <end position="151"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF98595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="155" mass="17402" checksum="1A8EB873F301C389" modified="2024-11-27" version="1">MSLTQIARNCGRLAATLGTKRMASGAVIQCQAVRMMHRIAVPAVANQVSQELASSRCRWQTQSVRRYSAKPPLSLKLINERVLLVLKLYDKIDPSKLNVESHFINDLGLDSLDHVEVIMAMEDEFGFEIPDSDAEKLLKPADIIKYVADKEDVYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTE3</accession>
    <name>A0AAU9FTE3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FGGY carbohydrate kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_07226</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGGY kinase family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome J"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="F:D-ribulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019321">
        <property type="term" value="P:pentose metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07782">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000101">
        <property type="entry name" value="FGGY carbohydrate kinase domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006003">
        <property type="entry name" value="FGGY_RbtK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01315">
        <property type="entry name" value="5C_CHO_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435:SF4">
        <property type="entry name" value="FGGY CARBOHYDRATE KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435">
        <property type="entry name" value="RIBULOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="5">
        <location>
            <begin position="7"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="6">
        <location>
            <begin position="283"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00074355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF99287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="548" mass="59670" checksum="B58EA5E094FCECDB" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTE9</accession>
    <name>A0AAU9FTE9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc finger MIZ domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_06718</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTE9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16791">
        <property type="entry name" value="SP-RING_ZMIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057847">
        <property type="entry name" value="ZMIZ1/ZMIZ2_GBD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF4">
        <property type="entry name" value="TONALLI, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25527">
        <property type="entry name" value="GBD-like_ZMIZ1_ZMIZ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="6">
        <location>
            <begin position="725"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="140"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="833"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="920"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1108"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="18"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="851"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="920"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="945"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1004"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1118"/>
            <end position="1127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF98585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1136" mass="118928" checksum="6801E8D31EC67AA5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4G0</accession>
    <name>A0AAU9G4G0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cytoplasmic dynein 1 intermediate chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_02105</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G4G0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="term" value="C:cytoplasmic dynein complex"/>
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        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 INTERMEDIATE CHAIN-RELATED"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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            <dbReference type="EMBL" id="BFG02646.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="637" mass="71179" checksum="91DC278CF2C3009A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4G3</accession>
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            <fullName evidence="2">SprT-like domain-containing protein</fullName>
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        <name evidence="3" type="ORF">DMAD_02040</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">RF1</strain>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="P:DNA damage response"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SprT"/>
        <property type="match status" value="1"/>
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        <location>
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            <end position="974"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="196"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
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        </location>
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        <location>
            <begin position="471"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="659"/>
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        </location>
    </feature>
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        <location>
            <begin position="27"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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        <location>
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    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG02557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1050" mass="116840" checksum="14D1C06D9A4AD01E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4G4</accession>
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        <recommendedName>
            <fullName evidence="8">alanine transaminase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_02217</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-alanine + 2-oxoglutarate = pyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:19453"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
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    <comment type="similarity">
        <text evidence="7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.2" evidence="8"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G4G4"/>
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        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004021">
        <property type="term" value="F:L-alanine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1970:FF:000001">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000012">
        <property type="entry name" value="alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000010">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045088">
        <property type="entry name" value="ALAT1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751:SF29">
        <property type="entry name" value="ALANINE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="197"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="65"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG02808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="591" mass="65093" checksum="03140CE41DE6A5C1" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4G8</accession>
    <name>A0AAU9G4G8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_01938</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the POU transcription factor family. Class-4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G4G8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007608">
        <property type="term" value="P:sensory perception of smell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000230">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000007">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF70">
        <property type="entry name" value="INHIBITORY POU PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0552">Olfaction</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="7 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="15">
        <location>
            <begin position="80"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="176"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="178"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="160"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG02439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="254" mass="27732" checksum="BF27672395C75589" modified="2024-11-27" version="1">MDQLDMLDPTGQMTTLAPISESPLTPTHQHLHGSYHSMNHMMSHHHPGTLSGHTGGHHGHSAVHHPVITAAVAAAGLHPDTDTDPRELEAFAERFKQRRIKLGVTQADVGKALANLKLPGVGALSQSTICRFESLTLSHNNMIALKPILQAWLEEAEAQAKNKRRDPDAPSVLPAGEKKRKRTSIAAPEKRSLEAYFAVQPRPSGEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRIVSSVTPSMTGHGSAGFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4H0</accession>
    <name>A0AAU9G4H0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphodiesterase</fullName>
            <ecNumber evidence="9">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_02067</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Vital for female fertility. Required for learning/memory.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="9">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="9"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G4H0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007615">
        <property type="term" value="P:anesthesia-resistant memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048675">
        <property type="term" value="P:axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001661">
        <property type="term" value="P:conditioned taste aversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007619">
        <property type="term" value="P:courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046958">
        <property type="term" value="P:nonassociative learning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008355">
        <property type="term" value="P:olfactory learning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007614">
        <property type="term" value="P:short-term memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040040">
        <property type="term" value="P:thermosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000001">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040844">
        <property type="entry name" value="PDE4_UCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18100">
        <property type="entry name" value="PDE4_UCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0114">cAMP</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="11">
        <location>
            <begin position="226"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="579"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="579"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="612"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="635"/>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
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    </dbReference>
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        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="469"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="244"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="EMBL" id="BFG03385.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="497" mass="55514" checksum="9C0324B412C477BA" modified="2024-11-27" version="1">MANFDDTLQRARLAFASGKTRNVNFRRKQLENLLRCYEENENDIISALEADLRRPKQESLIVETEFMKNDIKHILYHLSDWVKAEKPSKSIINVMDDVQIYNDPFGVVLVIGAWNYPLQLLLVPVASAIAAGNCVVIKPSEIAGNCAKFIAEVIPKYLDNDCYPVVCGGPSETAELLKQRFDYIFYTGSTRVGKIIHAAANKHLTPTTLELGGKSPCYIDKSVELRTAVKRILWGKLINCGQTCIAPDYILCSKEMQDKFVAEAKDVLKEWYGENIQSSPDLSRVINTNNFQRLIGLMKSGRVAVGGKYDASERYIEPTILVDVKPNDPIMEEEIFGPILPIYNVESAYDAIKFINARESPLVLYIFTSETEVQNLFVNGTQSGGMCVNDTIMHYAVDVLPFGGVGMSGMGSYHGKYGFDTFTHKKSCLGKDLSAVGEKLASARYPPYSDRKGSFLSFLLRKRRPLPNFYLTHVLAVGLGVGLTVLANYYLQKNSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4H8</accession>
    <name>A0AAU9G4H8_DROMD</name>
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        <submittedName>
            <fullName evidence="9">Ataxin-1-like</fullName>
        </submittedName>
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        <name evidence="9" type="ORF">DMAD_02050</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
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            <taxon>Muscomorpha</taxon>
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        </lineage>
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    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="Ataxin_AXH_dom"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="AXH DOMAIN-CONTAINING PROTEIN"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AXH" evidence="8">
        <location>
            <begin position="19"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <end position="28"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG02572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="200" mass="22639" checksum="3FE1936330906A87" modified="2024-11-27" version="1">MEFVPNHSYKPYKMPPQQQQPHPQPDSSECFRKGAYIELSNGALRRVEDIRTEDFIQSALRSQLFDLREATVVRIDRGQLSHVTITFSYDTRYAKVNMDVLPGHPLFVYGQGWASCNPQLSFQLYELKCQQLQVGDICLSLVPREQIAPPPPPAPAPAPPAAAAAPPQVPHRGYHSYQMYAEMANFVAAYTQHVIDKMRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4H9</accession>
    <name>A0AAU9G4H9_DROMD</name>
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        <submittedName>
            <fullName evidence="19">Potassium voltage-gated channel protein eag</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_02225</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome A"/>
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        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:voltage-gated potassium channel activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01464">
        <property type="entry name" value="EAGCHANNEL"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="367"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="17">
        <location>
            <begin position="43"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="18">
        <location>
            <begin position="113"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="16">
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4I2</accession>
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        <recommendedName>
            <fullName evidence="9">Phosphodiesterase</fullName>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <text evidence="5">Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Vital for female fertility. Required for learning/memory.</text>
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        <text evidence="1">Monomer.</text>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4I9</accession>
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            <fullName evidence="5 13">Glutathione synthetase</fullName>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13557"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13558"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13 15">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the eukaryotic GSH synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.3" evidence="4 13"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG03019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G4I9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004363">
        <property type="term" value="F:glutathione synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1080.10:FF:000011">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.50:FF:000001">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000141">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1760:FF:000007">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1760">
        <property type="entry name" value="Glutathione synthase, substrate-binding domain superfamily, eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1080.10">
        <property type="entry name" value="Glutathione Synthetase, Chain A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005615">
        <property type="entry name" value="Glutathione_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014042">
        <property type="entry name" value="Glutathione_synthase_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014709">
        <property type="entry name" value="Glutathione_synthase_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037013">
        <property type="entry name" value="GSH-S_sub-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004887">
        <property type="entry name" value="GSH_synth_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01986">
        <property type="entry name" value="glut_syn_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130:SF0">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03917">
        <property type="entry name" value="GSH_synth_ATP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03199">
        <property type="entry name" value="GSH_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001558">
        <property type="entry name" value="GSHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="6 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="11 13 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 13 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutathione synthase substrate-binding" evidence="16">
        <location>
            <begin position="301"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="462"/>
            <end position="471"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="495"/>
            <end position="498"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="524"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="549"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="551"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="557"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR001558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG03019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="581" mass="63736" checksum="660B382DCF6120D2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4J4</accession>
    <name>A0AAU9G4J4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Matrix metalloproteinase-14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_02668</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="14">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G4J4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000022">
        <property type="entry name" value="Matrix metalloproteinase 1, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000007">
        <property type="entry name" value="stromelysin-3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF291">
        <property type="entry name" value="MATRIX METALLOPROTEINASE 1, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00024">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18" id="KW-0482">Metalloprotease</keyword>
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    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="129"/>
            <end position="286"/>
        </location>
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        <location>
            <begin position="321"/>
            <end position="366"/>
        </location>
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            <begin position="367"/>
            <end position="412"/>
        </location>
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        </location>
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            <position position="110"/>
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        </ligand>
    </feature>
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        </ligand>
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        </ligand>
    </feature>
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            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        </ligand>
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        </ligand>
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        </ligand>
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        </ligand>
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        </ligand>
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        <ligand>
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        </ligand>
    </feature>
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            <position position="223"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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            <note>catalytic</note>
        </ligand>
    </feature>
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        <location>
            <position position="259"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        </ligand>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        </ligand>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
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        <location>
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        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
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        <location>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
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        <location>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Eukaryota</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <strain evidence="4 5">RF1</strain>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01585">
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            <end position="397"/>
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        <location>
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    </feature>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50174"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03178.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
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</entry>
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    <accession>A0AAU9G5E8</accession>
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            <fullName evidence="8">Peroxisomal ATPase PEX6</fullName>
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            <fullName evidence="9">Peroxin-6</fullName>
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    </protein>
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        <name evidence="13" type="ORF">DMAD_02620</name>
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        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
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        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G5E8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="895" mass="100880" checksum="822415CD6EA9280C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEH7</accession>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
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        <subcellularLocation>
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        <subcellularLocation>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
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        <location>
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        <location>
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        <location>
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        <location>
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            <begin position="1493"/>
            <end position="1503"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022025"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00043944"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG06589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1601" mass="173692" checksum="B53BE4B14A401CA1" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEI0</accession>
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        </submittedName>
    </protein>
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        <name evidence="9" type="ORF">DMAD_04747</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MTSS family.</text>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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</entry>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Yamamoto D."/>
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</entry>
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        <recommendedName>
            <fullName evidence="2">calcium/calmodulin-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_13627</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="2"/>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GEJ2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007613">
        <property type="term" value="P:memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14086">
        <property type="entry name" value="STKc_CaMKII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000009">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000001">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000002">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013543">
        <property type="entry name" value="Ca/CaM-dep_prot_kinase-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08332">
        <property type="entry name" value="CaMKII_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="14"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG06659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="509" mass="57583" checksum="171204D3454DCFE9" modified="2024-11-27" version="1">MAAPAACTRFSDNYDIKEELGKGAFSIVKRCVQKSTGFEFAAKIINTKKLTARDFQKLEREARICRKLHHPNIVRLHDSIQEENYHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESVNHCHQNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGDHQAWFGFAGTPGYLSPEVLKKEPYGKSVDIWACGVILYILLVGYPPFWDEDQHRLYSQIKAGAYDYPSPEWDTVTPEAKNLINQMLTVNPNKRITASEALKHPWICQRERVASVVHRQETVDCLKKFNARRKLKGAILTTMLATRNFSSRSMITKKGDGSQVKESTDSSSTTLEDDDIKEDKKGIVDRSTTVISKEPEAARRQEIIKITEQLIEAINSGDFDGYTKICDPHLTAFEPEALGNLVEGIDFHKFYFENVLGKNCKAINTTILNPHVHLLGEEAACIAYVRLTQYIDKQGHAHTHQSEETRVWHKRDNKWQNVHFHRSASGKISGATTFDFIPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEJ3</accession>
    <name>A0AAU9GEJ3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_04549</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GEJ3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="128"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="327"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="367"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG05936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="466" mass="52642" checksum="D5E4B846FACC0AED" modified="2024-11-27" version="1">MGNDDLRRRKTPCGFCMAVFKWIPVVFITAVIAWSYYAYVVELCIRNAENLVGMIFMLFFYHIALILFMWSYWRTIMTSVGRVPDQWRIPDEEVTRLFRADNPETQKRILNSFARSLPVTNRTMNGSVRFCEKCKIIKPDRAHHCSVCNSCVLKMDHHCPWVNNCVNFYNYKFFVLFLGYALVYCLYVAFTTLHDFVQFWKYENNGYSEQGQLNGSGVGRFHILFLFFISIMFAISLVSLFGYHIYLVLVNRTTLESFRAPVFRVGGPDKNGFNLGRYANFCEVFGDDWQYWPLPIFTSRGDGFSYPTSTDQSGEAPTVQRYAAMGDTTTNRLDGNPTDKLIDAIPLDTTPNNHDQPPPQHQHPHQVRSQNQAQPQPQQARHQARPNSLNLQPALELPLANGQSSNCSTAAVPSSQMVVVNLGTLPEDANGSAVIIDMVGDHSGAAAGVAAPKDACRQPNGDVPLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEK0</accession>
    <name>A0AAU9GEK0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Protein dopey-1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_04580</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DOP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040314">
        <property type="entry name" value="DOP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056457">
        <property type="entry name" value="DOP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007249">
        <property type="entry name" value="DOP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056459">
        <property type="entry name" value="TPR_DOP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056458">
        <property type="entry name" value="TPR_DOP1_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042">
        <property type="entry name" value="DOPEY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042:SF24">
        <property type="entry name" value="PROTEIN DOPEY-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24598">
        <property type="entry name" value="DOP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04118">
        <property type="entry name" value="Dopey_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24601">
        <property type="entry name" value="TPR_DOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24597">
        <property type="entry name" value="TPR_DOP1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DOP1 N-terminal" evidence="8">
        <location>
            <begin position="17"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like middle TPR" evidence="9">
        <location>
            <begin position="327"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like TPR" evidence="11">
        <location>
            <begin position="1541"/>
            <end position="1898"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like C-terminal" evidence="10">
        <location>
            <begin position="2099"/>
            <end position="2445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="557"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="602"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="680"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1275"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1433"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="602"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="633"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="686"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1280"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1305"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1319"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1433"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1464"/>
            <end position="1475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG05975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2631" mass="294105" checksum="A0A42CFA5C723CEF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEK4</accession>
    <name>A0AAU9GEK4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_04560</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GEK4"/>
    <dbReference type="Proteomes" id="UP001500889">
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    </dbReference>
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        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF78">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 5 7" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000303-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG05951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="201" mass="22901" checksum="42BAA155FED84E68" modified="2024-11-27" version="1">MVSKVVAFYGLMVAIVCLTTNLTHRQLAVQLFELRWTLSVHSLSVLDTYHQWVDLRDFAGHVLIIVNIASQCGLTRQQYAGLGQLLERYWWRGLRILNFPCNQFGQQMPELDGQQMVDHLRQVEAKIGHVFAKIEVNGPEAAPLYKLLTRNRSHMRSSEIEWNFVKFLVDGRGQVYGRYGATEEPSVLGSDIELLLGKGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEK5</accession>
    <name>A0AAU9GEK5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_04510</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GEK5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043897113" evidence="2">
        <location>
            <begin position="20"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG05874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="466" mass="52937" checksum="8481DAD27B6C72EC" modified="2024-11-27" version="1">MCSKALLLLAAMLAMRCFGSDSSRRTLALACEEVDVTEVEDPDVDGDRAEAIVALENNRYSSKNNNNNKYNSNHNDYNYNYAFGDVDGTADSNDNEQNNDNYNYNYNKAAAKWQHQAALLQQQKLYGNPIHSIYDFSRSQPQPQHQQQQQQQRRVGRFVVEHQNPEQDQEQDSDNSSSKNKGDSNSQPQQEELLLERRSRISRRQPEAGQQQLYDNEYAAAVEAGAPDARVDIEGEEDYAVAAEGQDVDEDADELSEQLPYGIQNVRKRRVRSVMPPAQQLLAPGPNSDEVTYQSLCPTNRVTVKLDSGEYRPNHYVEVTCAHNYAPQPPRVHNYDYVYRGNDLLRALLAERGEKREICSAIGFACIQLNRTIHLIRLNDASGCWESETRTVPSGCECMWPKHSYGDIGFYHQTQKRGRGGVRGRVAPNLDYKPGVGYRQLTVRARSPKAGPRSRRDDFGYESYDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEK7</accession>
    <name>A0AAU9GEK7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Kinesin-like protein unc-104</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_04956</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for presynaptic maturation, has a role in axonal transport of dense-core vesicles carrying synaptic vesicle precursors, components required for the morphological transformation of axonal growth cones to mature boutons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048490">
        <property type="term" value="P:anterograde synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021700">
        <property type="term" value="P:developmental maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051222">
        <property type="term" value="P:positive regulation of protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040012">
        <property type="term" value="P:regulation of locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051960">
        <property type="term" value="P:regulation of nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010975">
        <property type="term" value="P:regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008582">
        <property type="term" value="P:regulation of synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22705">
        <property type="entry name" value="FHA_KIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01233">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000001">
        <property type="entry name" value="Kinesin family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000004">
        <property type="entry name" value="Kinesin-like protein isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000204">
        <property type="entry name" value="kinesin-like protein unc-104 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
        <property type="entry name" value="Kinesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049780">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF10">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12473">
        <property type="entry name" value="DUF3694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="15">
        <location>
            <begin position="3"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="1528"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="950"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="579"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG06448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1661" mass="188096" checksum="F007A454FF464A30" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEK8</accession>
    <name>A0AAU9GEK8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_04886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GEK8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043953206" description="MD-2-related lipid-recognition domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG06360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="21194" checksum="D06599E0D31E4A99" modified="2024-11-27" version="1">MYLVALVVPVLCGLVLCDPSVVYKMKNIDCLGVAPFSANPVCYIKPLNWNKAVAHMDVDLLKPLHNISVHIQIFKRDYSNQYQPFLVDVIVNLCDILSRRSFMPFGVIFMKIASRFSNFNHSCPFAGHLIARGAYVDESYVPISPLGLYKVNITIMENYPAKRPDHAGGFVWYVQAMTPVIRKKQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEL4</accession>
    <name>A0AAU9GEL4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Metabotropic glutamate receptor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_04594</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="750"/>
            <end position="768"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="780"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="822"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="868"/>
            <end position="891"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="903"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="935"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="710"/>
            <end position="975"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
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            <dbReference type="ARBA" id="ARBA00023180"/>
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            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00054813"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS50259"/>
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        <source>
            <dbReference type="EMBL" id="BFG05990.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1190" mass="130147" checksum="CFEF06993ED2A0CA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEL5</accession>
    <name>A0AAU9GEL5_DROMD</name>
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        <recommendedName>
            <fullName evidence="14">FXNA-like protease</fullName>
        </recommendedName>
    </protein>
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        <name evidence="18" type="ORF">DMAD_04775</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="18 19">RF1</strain>
        </source>
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        <cofactor evidence="1">
            <name>Zn(2+)</name>
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    </comment>
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        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    <dbReference type="PANTHER" id="PTHR12147:SF22">
        <property type="entry name" value="ENDOPLASMIC RETICULUM METALLOPEPTIDASE 1"/>
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    </dbReference>
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        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ERMP1_C"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Peptidase_M28"/>
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        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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            <end position="502"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
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            <end position="527"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="534"/>
            <end position="564"/>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
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            <end position="599"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="606"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="16">
        <location>
            <begin position="136"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum metallopeptidase 1-like C-terminal" evidence="17">
        <location>
            <begin position="633"/>
            <end position="859"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010918"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF22248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFG06216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="861" mass="97879" checksum="025A0B3478FB48BC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFF5</accession>
    <name>A0AAU9GFF5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Aquaporin AQPcic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_04797</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GFF5"/>
    <dbReference type="Proteomes" id="UP001500889">
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    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000020">
        <property type="entry name" value="Entomoglyceroporin 4, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF270">
        <property type="entry name" value="ENTOMOGLYCEROPORIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG06249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="249" mass="26803" checksum="71ED1B05D187321F" modified="2024-11-27" version="1">MKGSTLDKICCFLAELIGTGMLVFLGCMGCVKTDFFPNTHLQIILNFGLAVMICIQCFGCVSGAHLNPAVTVAAYIYELVTLPMAFVYCVAQMLGAFIGYGLLKVLLPEDTLGGGAGLCVTLPHSSVSHAQAFGIEFVITAILVIVCCGVWDPRNSKFHDSVAIRFGLAISCLACAAGPFTGASMNPARSFAPALWNSHFDSNWIYWLAPLSAAAVTAYAYKTVFRREVVEAEITSHEKLRQLEDVQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFF6</accession>
    <name>A0AAU9GFF6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Tau-tubulin kinase homolog Asator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_13658</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14017">
        <property type="entry name" value="STKc_TTBK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000481">
        <property type="entry name" value="Asator, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000358">
        <property type="entry name" value="Tau tubulin kinase 2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047916">
        <property type="entry name" value="TTBK_Asator-like_STKc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="201"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="553"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="708"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="830"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="976"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1270"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1304"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1359"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="708"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="725"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="836"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1359"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1386"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG06750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1395" mass="152486" checksum="8125AC5D95C94D46" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFF9</accession>
    <name>A0AAU9GFF9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">[histone H3]-trimethyl-L-lysine(9) demethylase</fullName>
            <ecNumber evidence="3">1.14.11.66</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_04883</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Probable histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(9)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60200"/>
            <dbReference type="Rhea" id="RHEA-COMP:15538"/>
            <dbReference type="Rhea" id="RHEA-COMP:15542"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JHDM3 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.66" evidence="3"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140681">
        <property type="term" value="F:histone H3K36me2/H3K36me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140684">
        <property type="term" value="F:histone H3K9me2/H3K9me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048512">
        <property type="term" value="P:circadian behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000048">
        <property type="entry name" value="Lysine-specific demethylase 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001994">
        <property type="entry name" value="Tramtrack, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
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    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="15" id="KW-0863">Zinc-finger</keyword>
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            <begin position="9"/>
            <end position="51"/>
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    </feature>
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            <begin position="1435"/>
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        <location>
            <begin position="449"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="16">
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="13">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00053408"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51184"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
    <sequence length="1535" mass="170227" checksum="B4221CB5375C48EB" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFG2</accession>
    <name>A0AAU9GFG2_DROMD</name>
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        <recommendedName>
            <fullName evidence="5 20">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="20">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_04967</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23956"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12788"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:53604"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52424"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
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            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 20">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Golgi apparatus membrane</location>
            <topology evidence="2 20">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="BFG06462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9GFG2"/>
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        <property type="component" value="Chromosome E"/>
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        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23462">
        <property type="entry name" value="beta-trefoil_Ricin_Pgant9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000047">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.90.550.10:FF:000021">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
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    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
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        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF131">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 20" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 20" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 20" id="KW-0430">Lectin</keyword>
    <keyword evidence="17 20" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="22">
        <location>
            <begin position="531"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="105"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BFG06462.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="656" mass="73789" checksum="A103651AF31876EB" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFG3</accession>
    <name>A0AAU9GFG3_DROMD</name>
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        <recommendedName>
            <fullName evidence="9">Protein kinase C-like 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05038</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GFG3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20831">
        <property type="entry name" value="C1_dGM13116p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08678">
        <property type="entry name" value="C2_C21orf25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039934">
        <property type="entry name" value="C2CD2/C2CD2L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21119">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21119:SF5">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="5">
        <location>
            <begin position="369"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="6">
        <location>
            <begin position="814"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="70"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="579"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="660"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="660"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="674"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="689"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="709"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG06576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="891" mass="96922" checksum="A10C6D3961B66833" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EPH1</accession>
    <name>A0AAU9EPH1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Complexin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_07765</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Positively regulates a late step in synaptic vesicle exocytosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complexin/synaphin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EPH1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046928">
        <property type="term" value="P:regulation of neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016079">
        <property type="term" value="P:synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.580:FF:000002">
        <property type="entry name" value="Complexin, isoform AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.580">
        <property type="entry name" value="Single Helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008849">
        <property type="entry name" value="Synaphin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705">
        <property type="entry name" value="COMPLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705:SF4">
        <property type="entry name" value="COMPLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05835">
        <property type="entry name" value="Synaphin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="28"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF88873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="139" mass="16217" checksum="8F42C1C5DF8B6239" modified="2024-11-27" version="1">MAAFIAKQMVGNQLSAVKGGDGGDDGDDKEKAEEEERERQEAIREAEDRRKEKHRKMEEEREKMRQDIRDKYNIKKKEEIVEAAPQEEPNPLMRKKKTPEELAAEAEQEELDDFTKLKNQIETQVNELKTQIEGKCVMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EPH6</accession>
    <name>A0AAU9EPH6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Low-density lipoprotein receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_07726</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EPH6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000105">
        <property type="entry name" value="Lipophorin receptor 1, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000002">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000015">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000034">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000008">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000113">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000030">
        <property type="entry name" value="Sortilin related receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
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    <keyword evidence="6" id="KW-0245">EGF-like domain</keyword>
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        <location>
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            <end position="1007"/>
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        <source>
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    <sequence length="1063" mass="117716" checksum="4E3157A5670F3406" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EPJ0</accession>
    <name>A0AAU9EPJ0_DROMD</name>
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            <fullName evidence="16">E3 ubiquitin-protein ligase MARCHF5</fullName>
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        <alternativeName>
            <fullName evidence="17">Membrane-associated RING-CH protein V</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">RING-type E3 ubiquitin transferase MARCHF5</fullName>
        </alternativeName>
    </protein>
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        <name evidence="23" type="ORF">DMAD_07732</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="23 24">RF1</strain>
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            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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        <text evidence="4">Protein modification; protein ubiquitination.</text>
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            <topology evidence="2">Multi-pass membrane protein</topology>
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        <subcellularLocation>
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    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
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    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
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        <location>
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    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
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        <location>
            <begin position="358"/>
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        <location>
            <begin position="1"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
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        <location>
            <begin position="394"/>
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    </feature>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BFF88833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="405" mass="44435" checksum="B5675A78523ED50B" modified="2024-11-27" version="1">MADNAAAPTADDATATDVNTDVSSPPPVTPLKSAEDHTTLIFTPAPGSGDAHVSTPENKDKAPTNATTSTTVLVGGENPESERCCWICFATDEDNRLAAWVKPCQCRGTTKWVHQSCLYRWIDEKTQKGNPLRTVSCPQCQTEYIIVLPQMGKLCGALEAMDNMIKRLSPFLAAGFFVGSLYWTAVTYGAVTFLQIVGHEHGMSIMEAGDPLVLLIGLPAIPVGLVLGRLIRWEDALLRLIRNRGTVVRKFPFVNLIYPSLTQEEQPVNSNPATPALSDPVSATRVFCGALLLPTISSIVGRILFDSVDNTLHRTLLGGLTFITVKGILKIYLKQKQYARRKKRRIVDYTEENIRNFMHRNTNNPAGGARQQQQPQQPQQPPVAQASRVAAAAARERERQHGDIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EPN9</accession>
    <name>A0AAU9EPN9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ankyrin repeat domain-containing protein 65</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_07767</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EPN9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="214"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF88878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="335" mass="37091" checksum="4E79821E06FCCD98" modified="2024-11-27" version="1">MASCSPAEAIESTGEPRWAGFEPQIVQQTINSVTLHWTPFQGALVYSVEKYKRRHGWQQVHWSSSSPIEIPSLEENFGHRLRIRALNLSADGRCYVTLAISPDVIARTEAALPTTNCLNRAIRKGQQFLVKRMLRRRPSLIEYPAPNGFLPLANAIMQGEMCIIDVLLSAGCSVHLGNPRNGRTPLHLAFFHGHLPSARILLNKKARLEATDCNGMTPTHCAVDANQMEMVRFALEAGANIEARDACGWTLLMRGVVMDAGLELIKLLVTYGADLAAVDGVGKSCSDLAKLYRRQEARDYFDKVQKFRLAKAAATAAAVPQEQPLEQAARKDFRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXY5</accession>
    <name>A0AAU9EXY5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein AAR2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">AAR2 splicing factor homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_08070</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAR2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EXY5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13778">
        <property type="entry name" value="Aar2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13777">
        <property type="entry name" value="Aar2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.20:FF:000001">
        <property type="entry name" value="protein AAR2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.550">
        <property type="entry name" value="Aar2, C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007946">
        <property type="entry name" value="AAR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033648">
        <property type="entry name" value="AAR2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038514">
        <property type="entry name" value="AAR2_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033647">
        <property type="entry name" value="Aar2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038516">
        <property type="entry name" value="AAR2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12689">
        <property type="entry name" value="A1 CISTRON SPLICING FACTOR AAR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12689:SF4">
        <property type="entry name" value="PROTEIN AAR2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05282">
        <property type="entry name" value="AAR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20981">
        <property type="entry name" value="AAR2_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAR2 N-terminal" evidence="6">
        <location>
            <begin position="22"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="AAR2 C-terminal" evidence="5">
        <location>
            <begin position="215"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF89279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="388" mass="43362" checksum="18B6AD64B9AE1208" modified="2024-11-27" version="1">MNKDNSSMNMDPQLALRLLADGAVLVVAGVPVGTEFGIDLCAYTIGPEFRGVKMIPPGVHYVWCASQGLYGDAAPRVGFVHYFHPNEILVREWDKDLEELRPRQIAEPEVERDRIRKNLAQLEKMLAAYDYRYVCPWKDLTGSVTEPCVDRCRPTLGTIRTNIELQSCPDADRPRGEPGAASSSGSKRNAAIRLLLDESELLPNLKPVEGTAPRFSAIPLRVPHDALPADVSRHAMDCIAAVDNLIDSFGSSDALIEELQLAYVFFLVGYSVESLAHWRKLLGLLAHSEAAVSKHRLTYMKYSEVLAHQLPYLPEELMVPSPHNSVFKDVRELLVNLHEGGLSVSAERLRKRLEKKLGWYFEGLLDEDPEDQPVIIELPEAQESPICM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXZ6</accession>
    <name>A0AAU9EXZ6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Ankyrin repeat and KH domain-containing protein mask</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_09492</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22404">
        <property type="entry name" value="KH-I_MASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000046">
        <property type="entry name" value="Ankyrin repeat and KH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000012">
        <property type="entry name" value="ankyrin repeat domain-containing protein 17 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000131">
        <property type="entry name" value="ankyrin repeat domain-containing protein 17 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000055">
        <property type="entry name" value="ankyrin repeat domain-containing protein 17 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051631">
        <property type="entry name" value="Ankyrin-KH/SAM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047373">
        <property type="entry name" value="KH-I_MASK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
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            <end position="540"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
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            <begin position="560"/>
            <end position="584"/>
        </location>
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            <end position="659"/>
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            <end position="713"/>
        </location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00703"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF91963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="713" mass="79922" checksum="A6632E6F30DC9AE2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYA5</accession>
    <name>A0AAU9EYA5_DROMD</name>
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        <submittedName>
            <fullName evidence="2">Calphotin</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">DMAD_08544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <location>
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            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="726"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="728"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="744"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF89889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="813" mass="79349" checksum="1462A8AFB765E05C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F1H3</accession>
    <name>A0AAU9F1H3_DROMD</name>
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        <recommendedName>
            <fullName evidence="18">Aminopeptidase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_09703</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
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            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="16 18">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Lipid-anchor</topology>
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    </comment>
    <comment type="similarity">
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
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        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
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        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
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        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 18 23" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 18" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6 18" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19" id="KW-0732">Signal</keyword>
    <keyword evidence="10 16 18" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043583257" description="Aminopeptidase" evidence="19">
        <location>
            <begin position="25"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="22">
        <location>
            <begin position="34"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="20">
        <location>
            <begin position="259"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="21">
        <location>
            <begin position="569"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="17">
        <location>
            <position position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BFF91419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="925" mass="106242" checksum="B2512F1F5B3EE2CA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F1H7</accession>
    <name>A0AAU9F1H7_DROMD</name>
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        <submittedName>
            <fullName evidence="12">Ankyrin repeat and KH domain-containing protein mask</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_09492</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
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        <property type="molecule type" value="Genomic_DNA"/>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
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            <taxon>Ephydroidea</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="term" value="F:carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="Carboxylic ester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="33"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039155"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF89524.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0AAU9F1J3</accession>
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        <recommendedName>
            <fullName evidence="8">Carboxylic ester hydrolase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_08277</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="6">
            <text>acetylcholine + H2O = choline + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17561"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
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        </reaction>
    </comment>
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        <text evidence="1 8">Belongs to the type-B carboxylesterase/lipase family.</text>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="term" value="F:acetylcholinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000997">
        <property type="entry name" value="Cholinesterase"/>
    </dbReference>
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        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00878">
        <property type="entry name" value="CHOLNESTRASE"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
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    </dbReference>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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            <begin position="31"/>
            <end position="539"/>
        </location>
    </feature>
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        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="349"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600997-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF89539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="563" mass="63549" checksum="80FF40451B594D7C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="3">BAH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_09729</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BFF91449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20397">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000021">
        <property type="entry name" value="Winged eye, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052429">
        <property type="entry name" value="BAH_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048924">
        <property type="entry name" value="BAHCC1-like_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056841">
        <property type="entry name" value="TNRC18_BAHCC1-like_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047419">
        <property type="entry name" value="Tudor_WGE"/>
    </dbReference>
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        <property type="entry name" value="PHD FINGER TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12505:SF24">
        <property type="entry name" value="PROTEIN WINGED EYE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21744">
        <property type="entry name" value="BAHCC1-like_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24912">
        <property type="entry name" value="SH3_TNRC18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="1755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="662"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="875"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
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        <location>
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            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
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        <location>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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</entry>
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</entry>
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            <begin position="311"/>
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            <begin position="339"/>
            <end position="352"/>
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        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <sequence length="364" mass="39540" checksum="42BD144CA2B6C115" modified="2024-11-27" version="1">MADVMEKSSAQPPSLPLQQQQQQQLQQEAAPAPQQQQQQQQPQPQQQQQQQQQQQQQKPEAPKQVPASVPVRANQEQKEGDNDSGVDESTQEKDRNGPISPSSPVKTPTSTSSKADKSGISRPPSATPSNKSASKSRSASKNRLLLKTPEPEPVKKVPMNKVQVGHAPSPNLKAVRSKIGSLDNATYKPGGGHVKIESKKIDIKAAPRIEAKNDKYMPKGGEKKIVTTKLQWNAKSKIGSLENTAYKPGGGDKKIETLKMDFKDKAKPKVGSKDNVKYQPGGGDIKDNNPKEIQTQKVEIKAQSKVGSLDNVKHRPGGGEKKIFDDKDYLKNVEHSAALTTPPTQSPLPSMTASGADENLNQQS</sequence>
</entry>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        </source>
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            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="49839" checksum="29E22BA17ED3EE56" modified="2024-11-27" version="1">MASKKKIKAKETDAGYGSGEFSLAWNVDDYRTEYESEEHWDLRRSFMVAHKERFDEDRLVCLAQTFVNMEFLGCKYPSETMHLVAEMSKEIAEEFRRKRDQRLKRTFVSASDAAEQRAKGRSGTTKPNQRQDEQRGGTNPYSRERQCFGGGKPIDLFEGLRFGKLILYLAGGRSCLRNSCAISQVKYEERPCKEPESTEKTKYSEILVNNEVIAVGQGESLKEAKIAAFKQALFLLQTHCYSIKLNASRQTIKVEKTINGVNINVTKETADSLGDPKLDETNKGYRMMRLMGWTGGGLGRQKQGREEPVGYLLKTNRSGLGGCNKPNVNLGDYRKMIQNYVKSDDMRDMQFEPTFSKEERASFHKIASKYGLRSGSYGNGESRRLLITKKANHKTILQEVLSSRNPKFCERYFVQVPMQKAHLFPGHVASLDLEGLDD</sequence>
</entry>
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    <accession>A0AAU9F9Y1</accession>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <strain evidence="4 5">RF1</strain>
        </source>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
    </feature>
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        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
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        </source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="1071" mass="118833" checksum="BE7749A314CB3F7D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9Y5</accession>
    <name>A0AAU9F9Y5_DROMD</name>
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            <fullName evidence="2">Homeobox protein Hmx</fullName>
        </submittedName>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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            <begin position="42"/>
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        </location>
    </feature>
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        <location>
            <begin position="88"/>
            <end position="126"/>
        </location>
    </feature>
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        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
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        <location>
            <begin position="101"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF92506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="126" mass="13449" checksum="360FDFF8B9C47567" modified="2024-11-27" version="1">MPPRTPAPLLLRHCSMDADSDEEKERMALDEDDAAIDVEADEEHDAETMHETTPMRSSTPLTLPLSLSLAMPLAEMCTKSGNCLERNESLATGSTQSMPMDATSTTTGSSAAASGDADKSINQKAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9Y6</accession>
    <name>A0AAU9F9Y6_DROMD</name>
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        <submittedName>
            <fullName evidence="5">Neural cell adhesion molecule 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_10516</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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            <strain evidence="8 9">RF1</strain>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24411:SF55">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Diptera</taxon>
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            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <strain evidence="13 14">RF1</strain>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="5">procollagen-proline 4-dioxygenase</fullName>
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        <name type="scientific">Drosophila madeirensis</name>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="2">Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.</text>
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        <cofactor evidence="1">
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        <subcellularLocation>
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        <text evidence="4">Belongs to the P4HA family.</text>
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    </dbReference>
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        <property type="protein sequence ID" value="BFF93080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBK4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000011">
        <property type="entry name" value="Prolyl alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF244">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0847">Vitamin C</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044713092" description="procollagen-proline 4-dioxygenase" evidence="13">
        <location>
            <begin position="21"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="14">
        <location>
            <begin position="402"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF93079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="519" mass="59479" checksum="A0C2CCD1A2AE9F95" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBK6</accession>
    <name>A0AAU9FBK6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Calcium-binding mitochondrial carrier protein Aralar1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_11005</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="12">Homodimer (via N-terminus).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBK6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015183">
        <property type="term" value="F:L-aspartate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043490">
        <property type="term" value="P:malate-aspartate shuttle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000416">
        <property type="entry name" value="Aralar1, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000396">
        <property type="entry name" value="Calcium-binding mitochondrial carrier protein Aralar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000004">
        <property type="entry name" value="Calcium-binding mitochondrial carrier protein Aralar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051028">
        <property type="entry name" value="Mito_Solute_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678:SF9">
        <property type="entry name" value="CALCIUM-BINDING MITOCHONDRIAL CARRIER PROTEIN ARALAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="103"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="174"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="338"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="437"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="529"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF93093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="689" mass="76008" checksum="5F93CC5ECFBA9A1B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBL5</accession>
    <name>A0AAU9FBL5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prolyl 4-hydroxylase subunit alpha-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_11000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBL5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prolyl 4-hydroxylase N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF93084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="172" mass="20078" checksum="52B164A15C5AA443" modified="2024-11-27" version="1">MVDYGSVLRKKISLMRRYIALMRVKEMEAESDREQYLGNPLHSYSLIHHMHFDWTNWRRLIEQPLGTEQISKMRALMPQIPKTEYWNAKQDLIHAPLAGVQFDPLDSLELALFAFDKDRPDLAEKWLSATLSGYQQLSPSEKELYELLNVVKESQVTKLSELVKTLNTNQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBL7</accession>
    <name>A0AAU9FBL7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Calcium-binding mitochondrial carrier protein Aralar1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_11005</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="12">Homodimer (via N-terminus).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBL7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015183">
        <property type="term" value="F:L-aspartate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043490">
        <property type="term" value="P:malate-aspartate shuttle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000416">
        <property type="entry name" value="Aralar1, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000396">
        <property type="entry name" value="Calcium-binding mitochondrial carrier protein Aralar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000004">
        <property type="entry name" value="Calcium-binding mitochondrial carrier protein Aralar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051028">
        <property type="entry name" value="Mito_Solute_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678:SF9">
        <property type="entry name" value="CALCIUM-BINDING MITOCHONDRIAL CARRIER PROTEIN ARALAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="89"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="160"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="324"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="423"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="515"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF93094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="675" mass="74523" checksum="4E7FD858080576B6" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBL9</accession>
    <name>A0AAU9FBL9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">G protein-activated inward rectifier potassium channel 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_10982</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBL9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000001">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000078">
        <property type="entry name" value="Putative Inward rectifier potassium channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF102">
        <property type="entry name" value="INWARDLY RECTIFYING POTASSIUM CHANNEL 1, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 12" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 12" id="KW-0813">Transport</keyword>
    <keyword evidence="5 12" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="15">
        <location>
            <begin position="34"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="16">
        <location>
            <begin position="190"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="488"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF93055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="530" mass="59548" checksum="CC6111CF5BAC447A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBM2</accession>
    <name>A0AAU9FBM2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ferritin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_11014</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
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        <text evidence="1 6">Belongs to the ferritin family.</text>
    </comment>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:iron ion transport"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ferritin_CS"/>
    </dbReference>
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        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ferritin"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FERRITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0409">Iron storage</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
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        <location>
            <begin position="20"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Ferritin-like diiron" evidence="8">
        <location>
            <begin position="47"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="5">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Fe cation</name>
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            <label>1</label>
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        <source>
            <dbReference type="ARBA" id="ARBA00007513"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601519-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361145"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="224" mass="24935" checksum="9E943E38368EE676" modified="2024-11-27" version="1">MKLLVAFALIASLGALAQAEEEYCHNSVVTACSSSTFSANSICNARFAGIEHVEPEVQAYINSQLTKSYEYLLLATHFNSYQKNRPGFQKLYQGLSDRSFEDSIALIKQITKRGGIVDFNSRHESAASVSTKSPTLEVDELHSLALALDNEKQLATGATHIHTRAIHATERDPEMAHYIEEEYLGKQAESVRKLSGYANDLAKLMKVPDPSLSIYLFDEYLQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBM8</accession>
    <name>A0AAU9FBM8_DROMD</name>
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        <submittedName>
            <fullName evidence="9">Segmentation protein cap'n'collar</fullName>
        </submittedName>
    </protein>
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        <name evidence="9" type="ORF">DMAD_10980</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome O"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NUCLEAR FACTOR ERYTHROID 2-RELATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24411:SF55">
        <property type="entry name" value="SEGMENTATION PROTEIN CAP'N'COLLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
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            <end position="1413"/>
        </location>
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        <location>
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        </location>
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        </location>
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            <begin position="1316"/>
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        </location>
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        </location>
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        </location>
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        </location>
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        </location>
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        </location>
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        </location>
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        </location>
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        </location>
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        <location>
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        </location>
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            <end position="1363"/>
        </location>
    </feature>
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        <location>
            <begin position="1378"/>
            <end position="1400"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1413" mass="150956" checksum="7C78ECD63C2F2648" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBM9</accession>
    <name>A0AAU9FBM9_DROMD</name>
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        <recommendedName>
            <fullName evidence="5">procollagen-proline 4-dioxygenase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_10991</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the P4HA family.</text>
    </comment>
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    <dbReference type="EMBL" id="AP029263">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:iron ion binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="entry name" value="Prolyl 4-hydroxylase subunit alpha 2"/>
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        <property type="entry name" value="Prolyl 4-hydroxylase subunit alpha 2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="q2cbj1_9rhob like domain"/>
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    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
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        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
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        <property type="entry name" value="P4H_N"/>
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    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
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        <property type="entry name" value="TPR_P4H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF244">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23558">
        <property type="entry name" value="TPR_P4H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="14">
        <location>
            <begin position="370"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00002035"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00006511"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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            <dbReference type="ARBA" id="ARBA00023004"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="BFF93068.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="498" mass="57138" checksum="57E6D426E99BE02E" modified="2024-11-27" version="1">METFFDFISSKMDEYQREHADAASDITAYVSNPINAYLLTKRLTTDWRQVENLMEHDVGTDFLQNLTQYRNVLKFPSDEDLNGAAVALLRLQDTYQLDTSSVARGKLNGIQYSTEMSSDDCFELGRQSYVNNDYHHTVLWMNEAMSRMLEEPLNQTQTFTRADVLEYLAFSTYKEGNVESALVMTNELLQLLPNHERANGNKRFYEKEIAHQKEMKKMKGDDGTDEMPVSDLPVARSDTGELGVKERKSYEMLCRGELKPSPTYMRSLRCRYVTNNVPFLRLGPLKLEEAHKDPYIVIYHDAMYDSEMDLIKRMARPRFRRATVQNSVTGALETANYRISKSAWLKTDEDSVIAKVVQRTADMTGLDMESAEELQVVNYGIGGHYAPHFDFARREEKRAFEGLNLGNRIATVLFYMSDVEQGGATVFTTLRTALWPKRGTAAFWMNLHRDGEGDKRTQHAACPVLTGTKWVSNKWIHERGQEFRRPCALNEDSGDFAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBQ1</accession>
    <name>A0AAU9FBQ1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_11002</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBQ1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000049">
        <property type="entry name" value="DnaJ homolog subfamily C member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029827">
        <property type="entry name" value="JDP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44500">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44500:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="17"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00073114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF93087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="186" mass="20333" checksum="6C613C6A00C79A46" modified="2024-11-27" version="1">MSAVDAIINYKRSPNEDFYALLHCDETSSPEQIQAEYKVLALQYHPDKNSGDKEAEAKFQNLKEAKETLCDPEKRAVYDKWRNSGISMSYKQWLGMKEHVGQSMHWVTPKTKDRMLPETADGPGTGPGAGAGAGAGCSSGGLTAASPNPAHRRASEGGAALYYGGRKTEWGAENTDVANKFRNYEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBQ3</accession>
    <name>A0AAU9FBQ3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Calcium-binding mitochondrial carrier protein Aralar1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_11005</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="12">Homodimer (via N-terminus).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBQ3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015183">
        <property type="term" value="F:L-aspartate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043490">
        <property type="term" value="P:malate-aspartate shuttle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000416">
        <property type="entry name" value="Aralar1, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000396">
        <property type="entry name" value="Calcium-binding mitochondrial carrier protein Aralar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000004">
        <property type="entry name" value="Calcium-binding mitochondrial carrier protein Aralar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051028">
        <property type="entry name" value="Mito_Solute_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678:SF9">
        <property type="entry name" value="CALCIUM-BINDING MITOCHONDRIAL CARRIER PROTEIN ARALAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="167"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="344"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="443"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="535"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
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        <source>
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        <source>
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        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <sequence length="695" mass="76988" checksum="B93F501504D761D2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBQ9</accession>
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        <submittedName>
            <fullName evidence="5">Tropomodulin</fullName>
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    </protein>
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        <name evidence="5" type="ORF">DMAD_10985</name>
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    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5 6">RF1</strain>
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        </subcellularLocation>
    </comment>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:pointed-end actin filament capping"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TROPOMODULIN, ISOFORM N"/>
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    </dbReference>
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        <property type="entry name" value="Tropomodulin"/>
        <property type="match status" value="1"/>
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        <location>
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        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="536" mass="59739" checksum="B1094B0B50C6BA9C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FC21</accession>
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        <submittedName>
            <fullName evidence="12">Protein madd-4</fullName>
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        <name evidence="12" type="ORF">DMAD_11161</name>
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    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
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    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
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        </subcellularLocation>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000011">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000009">
        <property type="entry name" value="ADAMTS-like protein 3 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000107">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TSP1"/>
        <property type="match status" value="10"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="TSP-1 type 1 repeat"/>
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    </dbReference>
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        <location>
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    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
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        </location>
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        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
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        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF93281.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1429" mass="157907" checksum="A4BCFED5B56BECC2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FC25</accession>
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    <protein>
        <submittedName>
            <fullName evidence="3">Fibroleukin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_11098</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FC25"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043526904" evidence="2">
        <location>
            <begin position="23"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF93205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="112" mass="12819" checksum="7324E182E9103CDA" modified="2024-11-27" version="1">MAMNAWNCFVYIMLGVIFLASANSVDSLADIGVDQESCCSSSDLEDQCGFVCYPILKPLLQYLEKCQEMDLQIKELEDMFREQSVEIIKYKSKTEVLSKHLENNFCDTKAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJV0</accession>
    <name>A0AAU9FJV0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_13438</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJV0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="4"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="74"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF96191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="134" mass="15300" checksum="54EB641D5F8F98CB" modified="2024-11-27" version="1">MALESEIDQKLEKVQAEELKLERENEKLAKISIHPPPPKVLSYEEALLRNTNTLKSLELAKARLRSRITYSPVEKLLQQALENYRKELESLQATNEEANEAEEEQNLYELVMQNVFEASGKGSGKKQTSQSVDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJZ5</accession>
    <name>A0AAU9FJZ5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pancreatic lipase-related protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_13200</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJZ5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000238">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF178">
        <property type="entry name" value="LIPASE MEMBER H-A-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043515858" evidence="5">
        <location>
            <begin position="23"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="6">
        <location>
            <begin position="86"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF95894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="360" mass="40314" checksum="2858ACDF43D61BE9" modified="2024-11-27" version="1">MWKLCWLYVAVAIAWLAVGIRGEVALSQEDYNKTWIYMPNGEGKPEVAYLVEPPPENRINLPQLIKFELYGSDASLSSSADFWIDDNNFEFSQRHKRDTWQEMAEKFNPELDTKILVHGWKSSTMSNSIQSIRGAYIERGQVNVFAINWQDQADNIYYLTPARYTVQVGRAVAKLIDLLVEEKDADPQRIHLIGHSLGAHIMGYAGSYTKYRVNRITGLDPARPAFEDCIGPENHLDDTDANFVDVIHSCAGYLGFRKPIGMVDFYPNGGGPPQPGCKELSQIFTGCSHGRSYEYYAESINSPKGFYGVPCSGLDELKGKNCTGSKILMGDPVPRDARGIFFVKTSNKPSYALGIDEILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJZ8</accession>
    <name>A0AAU9FJZ8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enolase</fullName>
            <ecNumber evidence="3">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_13256</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="3"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJZ8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000002">
        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
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    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="3"/>
            <end position="134"/>
        </location>
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            <begin position="142"/>
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            <position position="210"/>
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            <position position="158"/>
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            <name>substrate</name>
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            <position position="245"/>
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            <end position="374"/>
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        <ligand>
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            <position position="395"/>
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        <ligand>
            <name>substrate</name>
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            <dbReference type="SMART" id="SM01193"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="433" mass="46626" checksum="A0455E40CEBBD200" modified="2024-11-27" version="1">MTIKAIKARQIYDSRGNPTVEVDLTTENGLFRAAVPSGASTGVHEALELRDNDKANYHGKSVLKAVAHVNDTLGPEVIKANLDVVDQAAVDNFMIKLDGTENKSKFGANAILGISLAVAKAGAAKKNVPLYKHIADLAGNKEIILPVPAFNVINGGSHAGNKLAMQEFMILPTGATTFTEAMKMGSEVYHHLKNVIKAKFGLDATAVGDEGGFAPNIQSNKEALVLITDAIAKAGYTGKIEIGMDVAASEFFKDGLYDLDFKNPASDKAQWLTPDKLANLYQEFIKEFPITSIEDPFDQDHWDAWTNLTSNTKIQIVGDDLTVTNPKRIATAVEKGACNCLLLKVNQIGTVTESIAAHLLAKKNGWGTMVSHRSGETEDSFIGDLVVGLSTGQIKTGAPCRSERLAKYNQILRIEEEIGSGVKFAGKSFRKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKA3</accession>
    <name>A0AAU9FKA3_DROMD</name>
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        <recommendedName>
            <fullName evidence="3">MABP1/WDR62 second WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_13277</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
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    </dbReference>
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        <location>
            <begin position="827"/>
            <end position="839"/>
        </location>
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        <location>
            <begin position="1042"/>
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        <location>
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        <location>
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            <end position="2249"/>
        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF24782"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF95986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2372" mass="260724" checksum="0DDA5D5463DC3B1F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKA4</accession>
    <name>A0AAU9FKA4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_13289</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
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    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKA4"/>
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        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of apoptotic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein polyubiquitination"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Apoptosis-resistant E3 ubiquitin protein ligase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000013">
        <property type="entry name" value="Apoptosis-resistant E3 ubiquitin protein ligase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000975">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
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        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
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        <property type="entry name" value="HECT_dom"/>
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        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
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    <dbReference type="InterPro" id="IPR058738">
        <property type="entry name" value="PH-like_AREL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF340">
        <property type="entry name" value="APOPTOSIS-RESISTANT E3 UBIQUITIN PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25916">
        <property type="entry name" value="AREL1_PH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="Filamin" evidence="6">
        <location>
            <begin position="302"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="628"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="930"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF96006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="963" mass="108706" checksum="DBAD971EA364D38A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKA8</accession>
    <name>A0AAU9FKA8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Cadherin-related tumor suppressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_13456</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF96214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome U"/>
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        <property type="term" value="C:apical plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0016327">
        <property type="term" value="C:apicolateral plasma membrane"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:cell adhesion molecule binding"/>
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        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
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        <property type="term" value="P:cytoskeleton organization"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:developmental growth"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001737">
        <property type="term" value="P:establishment of imaginal disc-derived wing hair orientation"/>
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    </dbReference>
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        <property type="term" value="P:establishment of ommatidial planar polarity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:positive regulation of hippo signaling"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0090251">
        <property type="term" value="P:protein localization involved in establishment of planar polarity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0022603">
        <property type="term" value="P:regulation of anatomical structure morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:regulation of developmental growth"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:regulation of tube length, open tracheal system"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Cadherin_FAT4_N"/>
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        <property type="entry name" value="Cadherin-like"/>
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        <property type="entry name" value="EGF/Laminin"/>
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        <property type="entry name" value="ASX_HYDROXYL"/>
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        <property type="entry name" value="LAM_G_DOMAIN"/>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <source>
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        <source>
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        <source>
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        <source>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="6">IST1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Charged multivesicular body protein 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_06989</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">ESCRT-III-like protein involved in cytokinesis, nuclear envelope reassembly and endosomal tubulation. Is required for efficient abscission during cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with CHMP1A, CHMP1B, VPS4A and VTA1. Interacts with SPAST, STAMBP, and USP8. May interact with VPS37B. May associate with the ESCRT-I complex. Interacts with MITD1, in competition with VSP4. Interacts with SPART (via MIT domain); leading to the recruitment of SPART to midbodies. Interacts with SPAST.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the IST1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FSK6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.60:FF:000001">
        <property type="entry name" value="IST1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.60">
        <property type="entry name" value="Vacuolar protein sorting-associated protein Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005061">
        <property type="entry name" value="Ist1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042277">
        <property type="entry name" value="IST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161">
        <property type="entry name" value="IST1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161:SF5">
        <property type="entry name" value="IST1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03398">
        <property type="entry name" value="Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="270"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="8"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="293"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="318"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="363"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFF98972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="375" mass="41325" checksum="ACC83BE9F6A6858B" modified="2024-11-27" version="1">MFSSGPNYNKLKTNLRLALNRLKLLEKKKAELTQKSRKEIADYLATGKTERARIRVEHIIREDYLVEAMEMVEMYCDLLLARFGLITQMKELDSGIAEPVASLVWVCPRLQSDIAELKIISDIFITKYGPQFAEHNRTATGDHSVSEKLMHKLTLQAPPKLLVENYLIAIAKNYNIEYEPDPQVMQEEQRPEHHLIDLSDRNNLSGSGGPPPQMGFIGYPAVPPLPNMPMPPTGKPFNYPPFGGGGGGGGGAAACAGPQPPPPFAYNIPPNQPPQPTMPSMVPGKCAEEKDLNTNFINRETNQTDDSGSPDENILLPPGGPTLPSAPPALDLPSLPNVPLDLPDIPPGPAGKKEDEDEIDFDELSRRFENLKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSK9</accession>
    <name>A0AAU9FSK9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Proton-coupled amino acid transporter-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_07054</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FSK9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF460">
        <property type="entry name" value="PROTON-COUPLED AMINO ACID TRANSPORTER 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="320"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="365"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="410"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="433"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="477"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="6">
        <location>
            <begin position="91"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF99056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="504" mass="55182" checksum="1575417A4EBFA6C2" modified="2024-11-27" version="1">MTLAVISGTGQACDTKIPTVVSSGYDSEKTQGGRNNASIGQPKFIRSDMADVPVQQAAGSTLPLVITRKKGGGDSDDGNYNPFEHRKVEHPTSDLETFVHLLKGSLGSGILAMPMAFSHAGLWFGLVATFAVGTLCTYCVHVLVKCAHILCRRRKIPMMGFADVAEQAFLDGPPALNRWSRFIRFMVNTFLVLDLLGCCCIYLVFVATNVEQVVSVYLETVLSVRVWIMIVTVPLIFMCLVRNLKFLTPFSMIANILMFVGIVITFIYMFTDLPAPSERPGLVAVSEWPLFFGTVIFALEGIGVVMSLENDMRNPTHFIGCPSVLNFGMGLVIALYTLVGFFGFLKYGPDTEASITLNLPVEDKLAQSVKLMIAIAIFFTFTLQFYVPVSILWKGLEHKIRPERQNISEYGLRVALVVLCGGIAVALPNLGPFISLIGAVCLSTLGMIVPATIELAVYHEDPGYGRFNWRLWKNSGLILFGVVGFVAGTYVSIIEFHAEFSGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSM4</accession>
    <name>A0AAU9FSM4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Molybdenum cofactor biosynthesis protein 1</fullName>
            <ecNumber evidence="6">4.1.99.22</ecNumber>
            <ecNumber evidence="7">4.6.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_06710</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Isoform MOCS1A and isoform MOCS1B probably form a complex that catalyzes the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP). MOCS1A catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate and MOCS1B catalyzes the subsequent conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cPMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Isoform MOCS1A and isoform MOCS1B probably form a heterooligomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the MoaC family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="6"/>
    <dbReference type="EC" id="4.6.1.17" evidence="7"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FSM4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21117">
        <property type="entry name" value="Twitch_MoaA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="molybdenum cofactor biosynthesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_B">
        <property type="entry name" value="MoaA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
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        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013483">
        <property type="entry name" value="MoaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040064">
        <property type="entry name" value="MoaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="MoaA_twitch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02666">
        <property type="entry name" value="moaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF0">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01383">
        <property type="entry name" value="cyclic_pyranopterin_phosphate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="21">
        <location>
            <begin position="56"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00015273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00054222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00063038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="BFF98575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="376" mass="42611" checksum="1B651E83B2036F24" modified="2024-11-27" version="1">MRLLVKYANRLLCQTAAPVPLRQKASAAATGSLKLATSAQTELERANKQDSPLTDTFGRHHTYLRISLTERCNLRCDYCMPADGVPLQPKDKLLTTEEILRLARIFVEQGVRKIRLTGGEPTIRRDIVDLVAEMKALPQLEHVGITTNGLVLTRLLLPLQRAGLDSLNISLDTLQKDRFEKITRRKGWERVIAGIDLAVQLGYRPKVNCVLMRNFNEDEVCDFVEFTRERPVDVRFIEYMPFSGNKWHTDRLISFKETVELIRQRWPEFEALPNGPNDTSKAFAVPGFKGQVGFITSMTEHFCGSCNRLRLTADGNIKVCLFGNKEFSLRDAMRSNVSEEDLVDLIGAAVRRKKRQHAGMLNLSQMENRPMILIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSN0</accession>
    <name>A0AAU9FSN0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Monocarboxylate transporter 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_06680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FSN0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Monocarboxylate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF286">
        <property type="entry name" value="GH22266P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="433"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="488"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="524"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="554"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="621"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF98538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="651" mass="70503" checksum="A12BC893AFAFC3F0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSN2</accession>
    <name>A0AAU9FSN2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Arginine-glutamic acid dipeptide repeats protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_06738</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11661">
        <property type="entry name" value="SANT_MTA3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000052">
        <property type="entry name" value="Arginine-glutamic acid dipeptide (RE) repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.50:FF:000004">
        <property type="entry name" value="arginine-glutamic acid dipeptide repeats protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000949">
        <property type="entry name" value="ELM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13859">
        <property type="entry name" value="ATROPHIN-RELATED"/>
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        <property type="entry name" value="FCH_dom"/>
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        <property type="entry name" value="FCHSD_SH3_1"/>
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        <property type="entry name" value="SH3-like_dom_sf"/>
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        <property type="entry name" value="SH3_domain"/>
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        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF21">
        <property type="entry name" value="PROTEIN NERVOUS WRECK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
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    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
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    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="12">
        <location>
            <begin position="11"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="545"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="638"/>
            <end position="701"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="361"/>
            <end position="380"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="431"/>
            <end position="542"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="703"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="749"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="931"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="497"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="713"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="789"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="822"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="877"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="936"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="968"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1002"/>
            <end position="1016"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF99116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1016" mass="111466" checksum="79BE213A6B9294CB" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSR9</accession>
    <name>A0AAU9FSR9_DROMD</name>
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        <recommendedName>
            <fullName evidence="5">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="5">3.13.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_07070</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine</text>
            <dbReference type="Rhea" id="RHEA:21708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 5">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="4 5">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the adenosylhomocysteinase family.</text>
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    <dbReference type="EC" id="3.13.2.1" evidence="5"/>
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        <property type="protein sequence ID" value="BFF99079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9FSR9"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000002">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000007">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000035">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000009">
        <property type="entry name" value="Adenosylhomocysteinase like 2"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
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        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
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    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
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    <dbReference type="NCBIfam" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF0">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="8">
        <location>
            <begin position="280"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="311"/>
            <end position="316"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="435"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF99079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="521" mass="57187" checksum="893302118AA32C71" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FT21</accession>
    <name>A0AAU9FT21_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Bumetanide-sensitive sodium-(Potassium)-chloride cotransporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_06848</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>K(+)(out) + 2 chloride(out) + Na(+)(out) = K(+)(in) + 2 chloride(in) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72395"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:72396"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC12A transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FT21"/>
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        </source>
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</entry>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
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            </authorList>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
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            <end position="118"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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</entry>
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        </source>
    </evidence>
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</entry>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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                <person name="Yamamoto D."/>
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    </evidence>
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</entry>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">RF1</strain>
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</entry>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4N8</accession>
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            <taxon>Drosophilidae</taxon>
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            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the spectrin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045169">
        <property type="term" value="C:fusome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016328">
        <property type="term" value="C:lateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008091">
        <property type="term" value="C:spectrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045170">
        <property type="term" value="C:spectrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030506">
        <property type="term" value="F:ankyrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042062">
        <property type="term" value="P:long-term strengthening of neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048790">
        <property type="term" value="P:maintenance of presynaptic active zone structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21246">
        <property type="entry name" value="CH_SPTB-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21248">
        <property type="entry name" value="CH_SPTB_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10571">
        <property type="entry name" value="PH_beta_spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000020">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000003">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000004">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000011">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000018">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000019">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000028">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000033">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000083">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000229">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000243">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000299">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000024">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041681">
        <property type="entry name" value="PH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001605">
        <property type="entry name" value="PH_dom-spectrin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016343">
        <property type="entry name" value="Spectrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15410">
        <property type="entry name" value="PH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002297">
        <property type="entry name" value="Spectrin_beta_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00683">
        <property type="entry name" value="SPECTRINPH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0117">Actin capping</keyword>
    <keyword evidence="8 10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="14">
        <location>
            <begin position="50"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="14">
        <location>
            <begin position="169"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="2147"/>
            <end position="2259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2097"/>
            <end position="2165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2262"/>
            <end position="2291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="995"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1281"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1628"/>
            <end position="1655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="2097"/>
            <end position="2140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="2154"/>
            <end position="2164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="2262"/>
            <end position="2275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR002297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG02883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2291" mass="265675" checksum="74B151E7A023571D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4N9</accession>
    <name>A0AAU9G4N9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cytoplasmic dynein 1 intermediate chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_02105</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G4N9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000552">
        <property type="entry name" value="Cytoplasmic dynein 1 intermediate chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001109">
        <property type="entry name" value="Cytoplasmic dynein 1 intermediate chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025956">
        <property type="entry name" value="DYNC1I1/DYNC1I2"/>
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        <property type="entry name" value="Dynein_IC"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF22">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 INTERMEDIATE CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11540">
        <property type="entry name" value="Dynein_IC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="489"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="17"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG02647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="629" mass="70028" checksum="856B2CA6DDD0B089" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4Q5</accession>
    <name>A0AAU9G4Q5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Thioredoxin-related transmembrane protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Protein disulfide-isomerase TMX1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Thioredoxin domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">DMAD_02463</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="19">Interacts with ATP2A2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G4Q5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000117">
        <property type="entry name" value="thioredoxin-related transmembrane protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052454">
        <property type="entry name" value="TMX_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46107">
        <property type="entry name" value="DUMPY: SHORTER THAN WILD-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46107:SF3">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="18" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 25" id="KW-0732">Signal</keyword>
    <keyword evidence="7 24 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043392558" description="Thioredoxin-related transmembrane protein 1" evidence="25">
        <location>
            <begin position="28"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="187"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="26">
        <location>
            <begin position="16"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="245"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="245"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="289"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00072260"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00075863"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00076905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="BFG03134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="332" mass="37277" checksum="8E2C853788727A94" modified="2024-11-27" version="1">MQCNSQLVAASCVIAVLMLVTAQSTHAATSQAGLQPGGKLIELDEDNWHLMLKGEWMIEFFAPWCPACKNLAPTWERFARVAKDVKVEVAKIDVTTSPSLSGRFFVTALPTIYHVKDGEFRQYRGARDGDALLHFLKKQQWQSIEPLSAWKKPDTIHMSVLSYFFKLSHTLKDFNGRLQEEYGLPTWGSYALFAIATIFVGAALGLLLVCLVDFVYPPKKSQRRSFSESQDNLAEGVEDLATEEIEDDGEAADDDEEQPGDSVEEEEEEEDDEDESSTKDSNDVPVFQKEAHKVVDELEKIEKEVANQSGDAGLEKLEKTDQVRKRKPRKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4R4</accession>
    <name>A0AAU9G4R4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Tyrosine-protein kinase-like otk</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_02706</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tyrosine-protein kinase-like otk"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF253">
        <property type="entry name" value="DPR-INTERACTING PROTEIN ETA, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
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    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043773411" evidence="7">
        <location>
            <begin position="22"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="23"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="154"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="238"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="384"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG03452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="429" mass="46859" checksum="6774E05C2C4E60D8" modified="2024-11-27" version="1">MNGRRLFSSGLALSLIFCCCASPVSLGDVSITKGPPATQTVKEQSGLQLPCNYQLPAGYRQSSSVILRWRKDNKTLRQAELGQASSTTSEPQLESMLREDARLTLNKQSGALLFASVLASDAGKYQCQLLVEGEMAASSSSGLLEVIEQLKFVPQPTSKNLELGSYSKVHCKAQGTPPPQVKWMRETQLPLPTNVTDQNGTLIFSQVSNDQRGQYTCFASNSQGQISATVSIGVVVAPKFSVPPEGPIETTEQGTVVMHCQAIGEPKPTIQWDKDLAYLNENNTDGERFTLMENGTVEIRNVQAEDEGRYGCTIGSSAGLKRAEVLLVVKSAKSASNSIVTRIIIVIICLAFLYFVLVLGLKVWYRYRRHLGKVQLEDATHAPTCDGHEHAEHEPCLTEANNSSKNLKSNKLRESPILEQESQVADEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4S1</accession>
    <name>A0AAU9G4S1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase M12B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_02473</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04272">
        <property type="entry name" value="ZnMc_salivary_gland_MPs"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sol narae, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1620.60:FF:000007">
        <property type="entry name" value="Sol narae, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041645">
        <property type="entry name" value="ADAMTS_CR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
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    </dbReference>
    <dbReference type="InterPro" id="IPR034030">
        <property type="entry name" value="ZnMc_salivary_gland_MPs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF249">
        <property type="entry name" value="SOL NARAE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17771">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13582">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043795961" description="Peptidase M12B domain-containing protein" evidence="10">
        <location>
            <begin position="21"/>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
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        <location>
            <begin position="840"/>
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        </location>
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        <location>
            <begin position="268"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="639"/>
            <end position="677"/>
        </location>
    </feature>
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        <location>
            <begin position="693"/>
            <end position="710"/>
        </location>
    </feature>
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        <location>
            <begin position="849"/>
            <end position="859"/>
        </location>
    </feature>
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        <location>
            <position position="464"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG03149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="898" mass="100641" checksum="EF355314906A32C1" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G521</accession>
    <name>A0AAU9G521_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cytoplasmic dynein 1 intermediate chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_02105</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G521"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000552">
        <property type="entry name" value="Cytoplasmic dynein 1 intermediate chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001109">
        <property type="entry name" value="Cytoplasmic dynein 1 intermediate chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025956">
        <property type="entry name" value="DYNC1I1/DYNC1I2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF22">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 INTERMEDIATE CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11540">
        <property type="entry name" value="Dynein_IC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="496"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="17"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="75"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG02645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="636" mass="70850" checksum="A508F58D102A2477" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G526</accession>
    <name>A0AAU9G526_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">HECT domain and RCC1-like domain-containing protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">HECT-type E3 ubiquitin transferase HERC2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_02088</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriole</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08664">
        <property type="entry name" value="APC10-HERC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14402">
        <property type="entry name" value="UBA_HERC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000015">
        <property type="entry name" value="E3 ubiquitin-protein ligase HERC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase HERC2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000006">
        <property type="entry name" value="E3 ubiquitin-protein ligase HERC2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000074">
        <property type="entry name" value="E3 ubiquitin-protein ligase HERC2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000004">
        <property type="entry name" value="E3 ubiquitin-protein ligase HERC2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000010">
        <property type="entry name" value="E3 ubiquitin-protein ligase HERC2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000006">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004939">
        <property type="entry name" value="APC_su10/DOC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021097">
        <property type="entry name" value="CPH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037976">
        <property type="entry name" value="HERC2_APC10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010606">
        <property type="entry name" value="Mib_Herc2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037252">
        <property type="entry name" value="Mib_Herc2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF408">
        <property type="entry name" value="OS09G0560450 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11515">
        <property type="entry name" value="Cul7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06701">
        <property type="entry name" value="MIB_HERC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM01337">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159034">
        <property type="entry name" value="Mib/herc2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51284">
        <property type="entry name" value="DOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51416">
        <property type="entry name" value="MIB_HERC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 13" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="647"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="698"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="752"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="802"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="domain" description="MIB/HERC2" evidence="19">
        <location>
            <begin position="1975"/>
            <end position="2048"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="16">
        <location>
            <begin position="2625"/>
            <end position="2671"/>
        </location>
    </feature>
    <feature type="domain" description="DOC" evidence="18">
        <location>
            <begin position="2926"/>
            <end position="3104"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="3134"/>
            <end position="3185"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="3186"/>
            <end position="3239"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="3239"/>
            <end position="3280"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="3281"/>
            <end position="3332"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="3335"/>
            <end position="3386"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="3387"/>
            <end position="3438"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="3439"/>
            <end position="3490"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="4220"/>
            <end position="4271"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="4272"/>
            <end position="4325"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="4326"/>
            <end position="4377"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="4378"/>
            <end position="4429"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="4432"/>
            <end position="4483"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="4484"/>
            <end position="4535"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="4536"/>
            <end position="4587"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="17">
        <location>
            <begin position="4727"/>
            <end position="5053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1457"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1711"/>
            <end position="1743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2053"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2457"/>
            <end position="2520"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2680"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="3587"/>
            <end position="3618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="4119"/>
            <end position="4162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="5062"/>
            <end position="5083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1475"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1732"/>
            <end position="1741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="2462"/>
            <end position="2476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="2492"/>
            <end position="2503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="2504"/>
            <end position="2515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="2701"/>
            <end position="2720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="4119"/>
            <end position="4148"/>
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    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="13">
        <location>
            <position position="5021"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFG02621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="5083" mass="548227" checksum="C09035E0798A3868" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G528</accession>
    <name>A0AAU9G528_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Heterogeneous nuclear ribonucleoprotein L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_02818</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G528"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12694">
        <property type="entry name" value="RRM2_hnRNPL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12424">
        <property type="entry name" value="RRM3_hnRNPL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12427">
        <property type="entry name" value="RRM4_hnRNPL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000072">
        <property type="entry name" value="heterogeneous nuclear ribonucleoprotein L isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000052">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein L like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000206">
        <property type="entry name" value="Smooth, isoform L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055204">
        <property type="entry name" value="HNRNPL_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021790">
        <property type="entry name" value="PTBP1-like_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22976">
        <property type="entry name" value="RRM_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11835">
        <property type="entry name" value="RRM_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="81"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="241"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFG03595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="475" mass="52014" checksum="1D0D31980A24CFB2" modified="2024-11-27" version="1">MPYNGASNGSGGAGGGGGGATIVVSEGPQNKKIRTGVQQPGENDVHMHARSTQQQNQQQALMNKSNDDLRRKRPETTRPNHILLFTIINPFYPITVDVLHKICHPHGQVLRIVIFKKNGVQAMVEFDNLDAATRARENLNGADIYAGCCTLKIDYAKPEKLNVYKNEPDTSWDYTLSTEPPLLGPGAAFPPFGAPEYHPTTPDNWKGASIHPTGLMKEPTGVVPGRNAPVAFTPQGQAQGAVMMVYGLDHDTSNTDKLFNLVCLYGNVARIKFLKTKEGTAMVQMGDSVAVERCVQHLNNIPVGTGGKIQIAFSKQNFLSEVINPFLLPDHTPSFKEYTGSKNNRFLSPAQASKNRIQPPSKILHFFNTPPGLTEDQLIGIFNIKDVPATSVRLFPLKTERSSSGLIEFPNISQAVLAIMKCNHLPIEGKGTKFPFIMKLCFSSSKSMNGAWNNATNEGMIEKENEVDSKVDIYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G537</accession>
    <name>A0AAU9G537_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Tenascin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_02418</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Synapse</location>
            <location evidence="14">Synaptosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Synaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tenascin family. Teneurin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG03073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097060">
        <property type="term" value="C:synaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099559">
        <property type="term" value="P:maintenance of alignment of postsynaptic density and presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048790">
        <property type="term" value="P:maintenance of presynaptic active zone structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034116">
        <property type="term" value="P:positive regulation of heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040017">
        <property type="term" value="P:positive regulation of locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001941">
        <property type="term" value="P:postsynaptic membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097090">
        <property type="term" value="P:presynaptic membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034110">
        <property type="term" value="P:regulation of homotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000331">
        <property type="term" value="P:regulation of terminal button organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051124">
        <property type="term" value="P:synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016200">
        <property type="term" value="P:synaptic target attraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048499">
        <property type="term" value="P:synaptic vesicle membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00053">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000021">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000013">
        <property type="entry name" value="Teneurin transmembrane protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000033">
        <property type="entry name" value="teneurin-a isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.180.10.10">
        <property type="entry name" value="RHS repeat-associated core"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057627">
        <property type="entry name" value="FN-plug_TEN1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056823">
        <property type="entry name" value="TEN-like_YD-shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056822">
        <property type="entry name" value="TEN_NHL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056820">
        <property type="entry name" value="TEN_TTR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051216">
        <property type="entry name" value="Teneurin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057629">
        <property type="entry name" value="Teneurin1-4_GBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028916">
        <property type="entry name" value="Tox-GHH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006530">
        <property type="entry name" value="YD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01643">
        <property type="entry name" value="YD_repeat_2x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219">
        <property type="entry name" value="TENEURIN AND N-ACETYLGLUCOSAMINE-1-PHOSPHODIESTER ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219:SF69">
        <property type="entry name" value="TENEURIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25024">
        <property type="entry name" value="EGF_TEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24329">
        <property type="entry name" value="FN-plug_TEN1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23093">
        <property type="entry name" value="GBD_Tenm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25021">
        <property type="entry name" value="TEN_NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="8"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="3"/>
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    <keyword evidence="9" id="KW-0524">Neurogenesis</keyword>
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            <end position="190"/>
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    <feature type="disulfide bond" evidence="17">
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            <begin position="833"/>
            <end position="842"/>
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        </source>
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            <dbReference type="ARBA" id="ARBA00009385"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00084038"/>
        </source>
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    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="BFG03073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="3023" mass="330991" checksum="39A4A9982D930051" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G540</accession>
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        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="13" type="ORF">DMAD_02379</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="1 15 16">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
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        <property type="entry name" value="LD34905P"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="229"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="274"/>
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        </location>
    </feature>
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        <location>
            <begin position="517"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="568"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
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        <location>
            <begin position="671"/>
            <end position="680"/>
        </location>
    </feature>
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        <location>
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        </location>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="742"/>
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    </feature>
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            <end position="1120"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG03030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1178" mass="126070" checksum="63EFFF271C9E3502" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G542</accession>
    <name>A0AAU9G542_DROMD</name>
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        <recommendedName>
            <fullName evidence="11">Ionotropic glutamate receptor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="9" type="ORF">DMAD_02824</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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    <dbReference type="AlphaFoldDB" id="A0AAU9G542"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF41">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG03610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="31581" checksum="680446B744E4B902" modified="2024-11-27" version="1">MLSATEQAAYGSILSPYSFDIPHAFVYNDSPSAGQLFCGPYLEMAMTFAEVNRYRLLLDELESLPKKSAIEQDIVNGQYDISMHGVLIRPVKEEKFAEEMRYSYPLELMMNCVMVPLDPELPKWMYVVWPLGKYIWTTLFLGIFYVAFLLRYLHWRETAQATRSYTRNMLQAMAILMFSPNMNMNLRLSQATLRVNLFYTLLFTLGFILSNYHSSHMTAYDMKPVFTRPIDSWSDLIISRLPIILPESLLEELRSLPIVDTSLLEPQFDSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEM2</accession>
    <name>A0AAU9GEM2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14114">
        <property type="entry name" value="STKc_Twitchin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000321">
        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000440">
        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000097">
        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000642">
        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000614">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001845">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001892">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000460">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000504">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000505">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000147">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002083">
        <property type="entry name" value="Protein CBR-UNC-22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000160">
        <property type="entry name" value="Titin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000003">
        <property type="entry name" value="Titin isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000012">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000127">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001003">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000249">
        <property type="entry name" value="twitchin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000056">
        <property type="entry name" value="twitchin isoform X4"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000936">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000006">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000775">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000805">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000831">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001034">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001164">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform G"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002180">
        <property type="entry name" value="Uncharacterized protein, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000466">
        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000876">
        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001033">
        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000935">
        <property type="entry name" value="Uncharacterized protein, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001001">
        <property type="entry name" value="Uncharacterized protein, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000051">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000553">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000559">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000748">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001016">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="78"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF151">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="40"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="38"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="9"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="119"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="228"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="335"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="438"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="543"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="640"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="746"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="1644"/>
            <end position="1732"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="1739"/>
            <end position="1823"/>
        </location>
    </feature>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0563">Paired box</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Paired" evidence="9">
        <location>
            <begin position="183"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="34"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="310"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="386"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="657"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="315"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="338"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="351"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="394"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="441"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG06649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="676" mass="72255" checksum="3F69D265A8BA66F2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEN9</accession>
    <name>A0AAU9GEN9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="39"/>
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        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14114">
        <property type="entry name" value="STKc_Twitchin_like"/>
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        <property type="entry name" value="Bent, isoform C"/>
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        <property type="entry name" value="Myosin-binding protein C, slow type"/>
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        <property type="entry name" value="Protein CBR-UNC-22"/>
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        <property type="entry name" value="Titin a"/>
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    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
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        <property type="entry name" value="TITIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="40"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="38"/>
    </dbReference>
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        <property type="entry name" value="IGc2"/>
        <property type="match status" value="28"/>
    </dbReference>
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        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="21"/>
    </dbReference>
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        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
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        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="9"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="119"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="228"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="335"/>
            <end position="426"/>
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    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="438"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="543"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="640"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="746"/>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
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            <taxon>Endopterygota</taxon>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="C1-like_sf"/>
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        <property type="entry name" value="C2CD2/C2CD2L"/>
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        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="5">
        <location>
            <begin position="358"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="6">
        <location>
            <begin position="783"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="70"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="568"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="649"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="649"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="663"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="678"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG06558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="860" mass="93526" checksum="898CD52621768375" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GET1</accession>
    <name>A0AAU9GET1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">FXNA-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_04772</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
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        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFG06211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:metalloexopeptidase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Endoplasmic reticulum metallopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ERMP1-like_C"/>
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    </dbReference>
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        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF22">
        <property type="entry name" value="ENDOPLASMIC RETICULUM METALLOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22248">
        <property type="entry name" value="ERMP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="416"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="454"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="492"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="519"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="559"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="588"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="16">
        <location>
            <begin position="147"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum metallopeptidase 1-like C-terminal" evidence="17">
        <location>
            <begin position="604"/>
            <end position="823"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF22248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFG06211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="825" mass="92350" checksum="36C7DDC805B3840E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GET4</accession>
    <name>A0AAU9GET4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">RNA polymerase II subunit A C-terminal domain phosphatase</fullName>
            <ecNumber evidence="2 9">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_04639</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">This promotes the activity of RNA polymerase II.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="2 9"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GET4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd17729">
        <property type="entry name" value="BRCT_CTDP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000040">
        <property type="entry name" value="RNA polymerase II subunit A C-terminal domain phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000007">
        <property type="entry name" value="RNA polymerase II subunit A C-terminal domain phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SD01014p"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039189">
        <property type="entry name" value="Fcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011947">
        <property type="entry name" value="FCP1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02250">
        <property type="entry name" value="FCP1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23081">
        <property type="entry name" value="RNA POLYMERASE II CTD PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23081:SF36">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT A C-TERMINAL DOMAIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="12">
        <location>
            <begin position="186"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="11">
        <location>
            <begin position="600"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="356"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="445"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="507"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="698"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="761"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="356"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="403"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="507"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="723"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="781"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="819"/>
            <end position="840"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG06041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="891" mass="99222" checksum="7006E6FF51AA4759" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GET8</accession>
    <name>A0AAU9GET8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">RNA-binding protein fusilli</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_05029</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESRP family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GET8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12738">
        <property type="entry name" value="RRM1_Fusilli"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12741">
        <property type="entry name" value="RRM2_Fusilli"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12743">
        <property type="entry name" value="RRM3_Fusilli"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000041">
        <property type="entry name" value="Epithelial splicing regulatory protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000070">
        <property type="entry name" value="Epithelial splicing regulatory protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000407">
        <property type="entry name" value="Fusilli, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050666">
        <property type="entry name" value="ESRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034980">
        <property type="entry name" value="Fusilli_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13976">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="393"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="787"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="834"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="787"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG06538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="875" mass="93914" checksum="22841AB8D2305325" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GF05</accession>
    <name>A0AAU9GF05_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">protein kinase C</fullName>
            <ecNumber evidence="7">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">DMAD_04829</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30 31">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="7"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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    <sequence length="1379" mass="152476" checksum="7E3DCD2BBE2D3D10" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="16 17">RF1</strain>
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        <property type="entry name" value="DUF1041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51258">
        <property type="entry name" value="MHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="452"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="592"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="MHD1" evidence="15">
        <location>
            <begin position="989"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1336"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1340"/>
            <end position="1359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG06628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1409" mass="158864" checksum="8A2904C8FAC444B5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GF08</accession>
    <name>A0AAU9GF08_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP synthase subunit beta</fullName>
            <ecNumber evidence="12">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_13628</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="12"/>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GF08"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18115">
        <property type="entry name" value="ATP-synt_F1_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01133">
        <property type="entry name" value="F1-ATPase_beta_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.170:FF:000004">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000026">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050053">
        <property type="entry name" value="ATPase_alpha/beta_chains"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184">
        <property type="entry name" value="ATP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184:SF71">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0139">CF(1)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="176"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFG06663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="364" mass="38406" checksum="4BE5E3AE1B732F50" modified="2024-11-27" version="1">MFALRAAAKADKNLLPFLGQLSRSHAAKAAVKAAAVANGKIVAVIGAVVDVQFDDDLPPILNALEVDNRSPRLVLEVAQHLGENTVRTIAMDGTEGLVRGQKVLDTGYPIRIPVGAETLGRIMNVIGEPIDERGPIPTDKTAPIHAEAPEFIEMSVEQEILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYNEMIEGGVISLKDKTSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGSMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFGITATAVSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GF11</accession>
    <name>A0AAU9GF11_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Protein enabled</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_05037</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ena/VASP family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GF11"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01207">
        <property type="entry name" value="EVH1_Ena_VASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000011">
        <property type="entry name" value="protein enabled isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000047">
        <property type="entry name" value="vasodilator-stimulated phosphoprotein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038023">
        <property type="entry name" value="VASP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014885">
        <property type="entry name" value="VASP_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202:SF22">
        <property type="entry name" value="PROTEIN ENABLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08776">
        <property type="entry name" value="VASP_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118370">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein, VASP, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0729">SH3-binding</keyword>
    <feature type="domain" description="RanBD1" evidence="11">
        <location>
            <begin position="136"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="WH1" evidence="12">
        <location>
            <begin position="153"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="309"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="433"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="313"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="374"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="448"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="491"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="513"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="536"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="575"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="627"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="715"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="743"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG06555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="840" mass="90147" checksum="8E01AE9D29E0F86A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GF12</accession>
    <name>A0AAU9GF12_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">diacylglycerol cholinephosphotransferase</fullName>
            <ecNumber evidence="13">2.7.8.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_04650</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1,2-dioctanoyl-sn-glycerol + CDP-choline = 1,2-dioctanoyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54232"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:76979"/>
            <dbReference type="ChEBI" id="CHEBI:78228"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:54233"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycerol + CDP-choline = 1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54332"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:74963"/>
            <dbReference type="ChEBI" id="CHEBI:82949"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:54333"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CDP-choline = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54244"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:73001"/>
            <dbReference type="ChEBI" id="CHEBI:75466"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:54245"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>CDP-choline + a 1,2-diacyl-sn-glycerol = a 1,2-diacyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32939"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:32940"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="12">Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.2" evidence="13"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GF12"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004142">
        <property type="term" value="F:diacylglycerol cholinephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004307">
        <property type="term" value="F:ethanolaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000002">
        <property type="entry name" value="Choline/ethanolamine phosphotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF37">
        <property type="entry name" value="BB IN A BOXCAR, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015665">
        <property type="entry name" value="CHOPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="382"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="390"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="408"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFG06054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="417" mass="46361" checksum="565303923057DFAE" modified="2024-11-27" version="1">MPLLAYKESHILSAQQLRKLSEHKYSCFSASLLDPLLQPWWNWLVSQTPLWLAPNLITIVGLILNIVTTLILICYSPNGVEAPPRWTCALCALGLFVYQSLDSIDGKQARRTNTSSPLGELFDHGCDSISTVFVALSACISCQLGHYPNWLFFQCFCAIALFYCAHWQTYVSGTMRFGRIDVTEAQFSIIAIHLVSAVLGPEFWLTKLPNFDCETRIMPLYLACGIDLLLTLRYAKCILTQGCGKNGSTVAGTSVLSPSIPLTLVVLPALIIAQKSPENLFTEHASVYIMAFGMVAAKITNKLVIAHMTKAEMEYLDWSLLGPALLFLNQYFNCIVPEIWLLWFTLIWGTQDLLRYCSQVCLEICQHLRINLFRIPYNPKGGAPHPATASVSSQSSFGSSVDKNGGTSHRKSKSKPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GF13</accession>
    <name>A0AAU9GF13_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Basic salivary proline-rich protein 1</fullName>
        </submittedName>
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    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1430:FF:000005">
        <property type="entry name" value="Eb1, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000007">
        <property type="entry name" value="Microtubule-associated protein, RP/EB family, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036133">
        <property type="entry name" value="EB1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140612">
        <property type="entry name" value="EB1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="14"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="12">
        <location>
            <begin position="217"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG06200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="299" mass="33534" checksum="26AD1181446FD32A" modified="2024-11-27" version="1">MAVNVYSTNVTSENLSRHDMLAWVNDCLQSQFSKIEELCTGAAYCQFMDMLFPNSVPVKRVKFRTNLEHEYIQNFKILQAGFKKMSVDKIIPVDKLVKGRFQDNFEFLQWFKKFFDANYDGREYEPLAQRGGVNMGSGKGPGGSSNGGSGVGSVHNDIHTMHRRGILAPQPKRLAPSVPAHEPAVSKVLPRTSSAVTHSRVATATNITGTVKKNDANHAVNTQQIDELSNQVMDMRLNLEGLEKERDFYFSKLRDIEILCQEAEEGETTPLVQKILDILYATEDGFAPPDDAPPEEEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GF54</accession>
    <name>A0AAU9GF54_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14114">
        <property type="entry name" value="STKc_Twitchin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000321">
        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000440">
        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000097">
        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000642">
        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000614">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001845">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001892">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000460">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000504">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000505">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000147">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002083">
        <property type="entry name" value="Protein CBR-UNC-22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000160">
        <property type="entry name" value="Titin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000003">
        <property type="entry name" value="Titin isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000012">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000127">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001003">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000249">
        <property type="entry name" value="twitchin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000056">
        <property type="entry name" value="twitchin isoform X4"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000936">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000006">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000775">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000805">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000831">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001034">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001164">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000567">
        <property type="entry name" value="Uncharacterized protein, isoform G"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002180">
        <property type="entry name" value="Uncharacterized protein, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000466">
        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000876">
        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001033">
        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000935">
        <property type="entry name" value="Uncharacterized protein, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001001">
        <property type="entry name" value="Uncharacterized protein, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000051">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000553">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000559">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000748">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001016">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="77"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF151">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="40"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="38"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Ephydroidea</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
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        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.</text>
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        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:protein N-linked glycosylation"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000001">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GATase_2_dom"/>
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        <property type="entry name" value="GFAT_N"/>
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        <property type="entry name" value="GlmS/AgaS_SIS"/>
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        <property type="entry name" value="GlmS/FrlB_SIS"/>
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        <property type="entry name" value="Ntn_hydrolases_N"/>
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        <property type="entry name" value="SIS_dom"/>
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        <property type="entry name" value="SIS_dom_sf"/>
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        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
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    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="8">
        <location>
            <begin position="2"/>
            <end position="280"/>
        </location>
    </feature>
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        <location>
            <begin position="352"/>
            <end position="492"/>
        </location>
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        <location>
            <begin position="523"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004775"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF88908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="674" mass="75683" checksum="EB3162DE99A4A88C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EQ00</accession>
    <name>A0AAU9EQ00_DROMD</name>
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        <recommendedName>
            <fullName evidence="8">Peptidoglycan-recognition protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="14" type="ORF">DMAD_07842</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        </lineage>
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    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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        </subcellularLocation>
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        <text evidence="2 8">Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.</text>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PEPTIDOGLYCAN-RECOGNITION PROTEIN LB"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="N-acetylmuramoyl-L-alanine amidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 8" id="KW-0391">Immunity</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043594328" description="Peptidoglycan-recognition protein" evidence="11">
        <location>
            <begin position="18"/>
            <end position="229"/>
        </location>
    </feature>
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        <location>
            <begin position="32"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="12">
        <location>
            <begin position="42"/>
            <end position="181"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
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        <location>
            <begin position="69"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR037945"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR037945-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00644"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF88983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="229" mass="25508" checksum="E25A3FA6E030AA60" modified="2024-11-27" version="1">MTALGLVLLSMMGYTQHMQQLQPSSVGVEGGARLISRADWGARVPKSVDYFEGPAPYVIIHHSYMPAACFSTAECMKSMRDMQDFHQVERGWNDIGYSFGIGGDGMIYTGRGFNVIGAHAPKYNDKSVGIVLIGDWRTELPPKQMLDAAKNLIAFGVFKGYIDPAYKLLGHKQVRDTECPGGRLFQEISTWPHFNNSIRLNTTTEQPQKVEPQPQQQHQPKTLPANPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYE3</accession>
    <name>A0AAU9EYE3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Golgi integral membrane protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_08569</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EYE3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="194"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="248"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="471"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="56"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="200"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="292"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="350"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="375"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="474"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF89934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="498" mass="54723" checksum="D112A5102F4CCCFE" modified="2024-11-27" version="1">MSTTRFARSGRVRICILMSMFVVLIMMIVIYQMSQHQLDESRAFQEGLNVQMQSEKLTAEKRMSVLRTEKEKYENQLAESIQKQQETERALQEKFDGQLEKYKLLEFKYSELEGDCVKSKKKHIEDTNSFDQKLQKLLADVHKDKASKEHEVAAWKEKHDKLQRESEHKIHALEATLTEYKSNCHYIPKVQPAEAPARTGVQQQLNKPAEMTPTTRHSSPSQQAHSIDKPRNDKSFNAQLQIVPKSLTEKQQQEEPQLAGGPVSPLSAPRKSSTAGAAVKSAGDAPNASVAPRLSSSSSGLPPLALPPAKPERKLPENVAPIPDNFEDTKVDAKGDAVDLDTAAEELPKNENNNRYANVVNDLESNKNLGVAPKPVEDSGAHEVKDAMNEPSFNLPAAGQNLFDGELPAPGPGPAADDAVKAEAAGGGDGDDDDDVGDVAVKQPQHLLDTDNLNNEIAADQGKEFAEGIHLEDAMDEDQDEDDYSNAARKKGGEAIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYE4</accession>
    <name>A0AAU9EYE4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Metalloendopeptidase</fullName>
            <ecNumber evidence="19">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_10076</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="18 19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="18 19">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="19"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008586">
        <property type="term" value="P:imaginal disc-derived wing vein morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032927">
        <property type="term" value="P:positive regulation of activin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030513">
        <property type="term" value="P:positive regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd04281">
        <property type="entry name" value="ZnMc_BMP1_TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000004">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000052">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000053">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000003">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000240">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015446">
        <property type="entry name" value="BMP_1/tolloid-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034036">
        <property type="entry name" value="ZnMP_TLD/BMP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF43">
        <property type="entry name" value="TOLLOID-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001199">
        <property type="entry name" value="BMP_1/tolloid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 18 19" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5 18 19" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="10 16 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="23">
        <location>
            <begin position="518"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="21">
        <location>
            <begin position="722"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="21">
        <location>
            <begin position="839"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="953"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="21">
        <location>
            <begin position="996"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="1113"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="21">
        <location>
            <begin position="1157"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="21">
        <location>
            <begin position="1270"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="64"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="243"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="388"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="388"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="404"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="424"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="active site" evidence="15 18">
        <location>
            <position position="614"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="613"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="617"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="623"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="583"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="585"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001199-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001199-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="BFF91908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1454" mass="163838" checksum="A19752EE3BC95B23" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F1M0</accession>
    <name>A0AAU9F1M0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Phosphatidylinositol-binding clathrin assembly protein LAP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_08306</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Binds clathrin and phosphatidylinositol 4,5-bisphosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PICALM/SNAP91 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F1M0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098894">
        <property type="term" value="C:extrinsic component of presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16985">
        <property type="entry name" value="ANTH_N_AP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000017">
        <property type="entry name" value="Phosphatidylinositol-binding clathrin assembly protein LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000001">
        <property type="entry name" value="phosphatidylinositol-binding clathrin assembly protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0168">Coated pit</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="12">
        <location>
            <begin position="16"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="620"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="700"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="722"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF89584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="742" mass="78753" checksum="6CA0B6C2B42F5288" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F1M3</accession>
    <name>A0AAU9F1M3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DBB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_09558</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F1M3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005104">
        <property type="term" value="F:fibroblast growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005068">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052446">
        <property type="entry name" value="B-cell_PI3K-Signaling_Adptrs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017893">
        <property type="entry name" value="DBB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16267">
        <property type="entry name" value="BANK1/PIK3AP1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16267:SF11">
        <property type="entry name" value="STUMPS, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14545">
        <property type="entry name" value="DBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01282">
        <property type="entry name" value="DBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51376">
        <property type="entry name" value="DBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DBB" evidence="3">
        <location>
            <begin position="427"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="22"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="678"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="738"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="887"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1031"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1080"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="939"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="680"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="707"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="746"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="896"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1031"/>
            <end position="1049"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF91229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1255" mass="138993" checksum="19BEC22CC5785CAC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F1M8</accession>
    <name>A0AAU9F1M8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lipid storage droplets surface-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_09772</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the perilipin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F1M8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010890">
        <property type="term" value="P:positive regulation of triglyceride storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004279">
        <property type="entry name" value="Perilipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024:SF49">
        <property type="entry name" value="LIPID STORAGE DROPLETS SURFACE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024">
        <property type="entry name" value="PERILIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03036">
        <property type="entry name" value="Perilipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="366"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="384"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF91509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="400" mass="44694" checksum="A255660CAB717FC8" modified="2024-11-27" version="1">MATATIGSGLHLEAIDRIGSIPLVESSVKRVENIYGKVKNNNRLFSWYFEAAEATISAAYGSVQPAVKLFEHPLKRLDNVMCKSLDILEQRIPLVYLPPEMEYMSDHLVRPVLKRADSVKQIGNAVLESPLTTYAADRIDGAFTAGDKFVDKYLVPISTDQDQTDAPQEDDNVAANEKGAIKAIHHGQRFSRKLKRRLTQRTIAEARALKKQSKEAIHVLIYAVELIATDPKQAMQRAKELWAYLSADEPENQARPATLEQLIVLLTRESARRVVHLVNFSATVASNIPRNLAHTTTEVAHQIVCINHRIITISRLDKVKSLSKEEAESLLKRALAFYGNLQGLTNSYLERVANFLSGRIEAEKVTGTDSATSNHRSSRRRQDNNTNNYSAAHNNINGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F1S8</accession>
    <name>A0AAU9F1S8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Guanylate cyclase</fullName>
            <ecNumber evidence="3 12">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_08389</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="3 12"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001653">
        <property type="term" value="F:peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000801">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000039">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF462">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="337"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="452"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="17">
        <location>
            <begin position="810"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="192"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="446"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1019"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1071"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1257"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="747"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1024"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1071"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1092"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1110"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1292"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1326"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1349"/>
            <end position="1361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFF89689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1375" mass="150675" checksum="A1109F75F9BA756E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F239</accession>
    <name>A0AAU9F239_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein hemingway</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_09112</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFAP97 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F239"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029488">
        <property type="entry name" value="Hmw/CFAP97"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13879">
        <property type="entry name" value="Hmw_CFAP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="141"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="401"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="58"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="101"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="199"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="415"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF90621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="607" mass="68444" checksum="D2ED76436BDADA91" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F245</accession>
    <name>A0AAU9F245_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="26">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">DMAD_08549</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30 31">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20 26">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphocholine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38583"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:38584"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 25">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="22">Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit ATP8B1 and an accessory beta subunit TMEM30A. The flippase ATP8B1:TMEM30A complex can form an intermediate phosphoenzyme in vitro. Also interacts with beta subunit TMEM30B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="26">Membrane</location>
            <topology evidence="26">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 26">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="26"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140327">
        <property type="term" value="F:flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005548">
        <property type="term" value="F:phospholipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000012">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000225">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF190">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 24 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 25 26" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 24 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15 26" id="KW-1278">Translocase</keyword>
    <keyword evidence="9 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 26" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="439"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="488"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1268"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1351"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1382"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1412"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1455"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="28">
        <location>
            <begin position="182"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="29">
        <location>
            <begin position="1237"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="22"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="127"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="605"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="649"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1498"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1569"/>
            <end position="1595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1610"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="27">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="620"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="723"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="27">
        <location>
            <begin position="1509"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="1573"/>
            <end position="1595"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="23">
        <location>
            <position position="565"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="565"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="565"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="566"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="567"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="567"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="828"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="869"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="891"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="925"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1005"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1006"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1007"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1185"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1191"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="1211"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1214"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="1215"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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</entry>
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6 7">RF1</strain>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="12">Homodimer (via N-terminus).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBN4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015183">
        <property type="term" value="F:L-aspartate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043490">
        <property type="term" value="P:malate-aspartate shuttle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000416">
        <property type="entry name" value="Aralar1, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000396">
        <property type="entry name" value="Calcium-binding mitochondrial carrier protein Aralar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000004">
        <property type="entry name" value="Calcium-binding mitochondrial carrier protein Aralar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051028">
        <property type="entry name" value="Mito_Solute_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678:SF9">
        <property type="entry name" value="CALCIUM-BINDING MITOCHONDRIAL CARRIER PROTEIN ARALAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="163"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="327"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="426"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="518"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF93097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="678" mass="74765" checksum="1C4774DF33E6F3C6" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBP3</accession>
    <name>A0AAU9FBP3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Prolyl 4-hydroxylase subunit alpha-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_11000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBP3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043403800" evidence="1">
        <location>
            <begin position="25"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Prolyl 4-hydroxylase N-terminal" evidence="2">
        <location>
            <begin position="32"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF93083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="219" mass="25292" checksum="CAD1A083CAEDA459" modified="2024-11-27" version="1">MWLSLRLGLVLVALSITFWVKANAERGSYSPSGIEELITVEKKLLAQMVDYGSVLRKKISLMRRYIALMRVKEMEAESDREQYLGNPLHSYSLIHHMHFDWTNWRRLIEQPLGTEQISKMRALMPQIPKTEYWNAKQDLIHAPLAGVQFDPLDSLELALFAFDKDRPDLAEKWLSATLSGYQQLSPSEKELYELLNVVKESQVTKLSELVKTLNTNQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBP6</accession>
    <name>A0AAU9FBP6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Neutral ceramidase</fullName>
            <ecNumber evidence="3 14">3.5.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_11003</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>an N-acylsphing-4-enine + H2O = sphing-4-enine + a fatty acid</text>
            <dbReference type="Rhea" id="RHEA:20856"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:57756"/>
            <dbReference type="EC" id="3.5.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the neutral ceramidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.23" evidence="3 14"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBP6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017040">
        <property type="term" value="F:N-acylsphingosine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046514">
        <property type="term" value="P:ceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2300:FF:000003">
        <property type="entry name" value="Neutral ceramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2300">
        <property type="entry name" value="Neutral/alkaline non-lysosomal ceramidase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006823">
        <property type="entry name" value="Ceramidase_alk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038445">
        <property type="entry name" value="NCDase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031331">
        <property type="entry name" value="NEUT/ALK_ceramidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031329">
        <property type="entry name" value="NEUT/ALK_ceramidase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12670">
        <property type="entry name" value="CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12670:SF1">
        <property type="entry name" value="NEUTRAL CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04734">
        <property type="entry name" value="Ceramidase_alk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17048">
        <property type="entry name" value="Ceramidse_alk_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="8 14" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043806986" description="Neutral ceramidase" evidence="15">
        <location>
            <begin position="24"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="Neutral/alkaline non-lysosomal ceramidase N-terminal" evidence="16">
        <location>
            <begin position="25"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Neutral/alkaline non-lysosomal ceramidase C-terminal" evidence="17">
        <location>
            <begin position="540"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="116"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="225"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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    <feature type="binding site" evidence="13">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="509"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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        <source>
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    </evidence>
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        <source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00048057"/>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF17048"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0AAU9FBT0</accession>
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        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="ADENOSINE RECEPTOR, ISOFORM A"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93137.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
    <sequence length="791" mass="87758" checksum="9067171A59F9F5CC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBU1</accession>
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        <alternativeName>
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        </alternativeName>
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        <name evidence="14" type="ORF">DMAD_11037</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Diptera</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
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        <subcellularLocation>
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        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
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    <comment type="similarity">
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        <property type="protein sequence ID" value="BFF93135.1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0035694">
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0035695">
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF16026">
        <property type="entry name" value="MIEAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
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        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019863"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032687"/>
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    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16026"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93135.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBU2</accession>
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        <name evidence="17" type="ORF">DMAD_11003</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>an N-acylsphing-4-enine + H2O = sphing-4-enine + a fatty acid</text>
            <dbReference type="Rhea" id="RHEA:20856"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:57756"/>
            <dbReference type="EC" id="3.5.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the neutral ceramidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.23" evidence="3 14"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBU2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017040">
        <property type="term" value="F:N-acylsphingosine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046514">
        <property type="term" value="P:ceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2300:FF:000003">
        <property type="entry name" value="Neutral ceramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2300">
        <property type="entry name" value="Neutral/alkaline non-lysosomal ceramidase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006823">
        <property type="entry name" value="Ceramidase_alk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038445">
        <property type="entry name" value="NCDase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031331">
        <property type="entry name" value="NEUT/ALK_ceramidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031329">
        <property type="entry name" value="NEUT/ALK_ceramidase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12670">
        <property type="entry name" value="CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12670:SF1">
        <property type="entry name" value="NEUTRAL CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04734">
        <property type="entry name" value="Ceramidase_alk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17048">
        <property type="entry name" value="Ceramidse_alk_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="8 14" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Neutral/alkaline non-lysosomal ceramidase N-terminal" evidence="15">
        <location>
            <begin position="33"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Neutral/alkaline non-lysosomal ceramidase C-terminal" evidence="16">
        <location>
            <begin position="548"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="478"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="517"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF17048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF93089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="712" mass="78868" checksum="07EDB35DDB44A929" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBU3</accession>
    <name>A0AAU9FBU3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDP-N-acetylglucosamine diphosphorylase</fullName>
            <ecNumber evidence="3">2.7.7.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_11079</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate + UTP + H(+) = UDP-N-acetyl-alpha-D-glucosamine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.23" evidence="3"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBU3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04193">
        <property type="entry name" value="UDPGlcNAc_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000075">
        <property type="entry name" value="Probable UDP-N-acetylglucosamine pyrophosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039741">
        <property type="entry name" value="UDP-sugar_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952:SF2">
        <property type="entry name" value="LD24639P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952">
        <property type="entry name" value="UDP- GLUCOSE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF93183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="483" mass="54619" checksum="847189F440B4A969" modified="2024-11-27" version="1">MTDYLSLQTRLAQVHQEHLLKFWHELTPDERADLVRDIEELNLDEIKQYFDRATVSMNENGIKLDDRLQPMPEGKVISIARTPKEQLHAYRDEGLRQISNGHVAVLLMAGGQGTRLGFDHPKGMYDVGLQSRKSLFRIQAERILKLEELAQEANEKRGNITWYIMTSEHTVQPTYDYFVANNFFGLKPEDVLLFEQGSLPCFEYDGRIILDEKHRVARAPDGNGGIYRAMRRQGILEDMQKRGVLYLHAHSVDNILIKVADPVFIGYCVHEKADCAAKVVEKAAPNEPVGVVAIVDGKYQVVEYSEISAKTAEMRNSDGRLTFSAGNICNHFFSSSFLQDIGSKFEQELKLHVAKKKIPFVDNAGKRLTPDKPNGIKIEKFVFDVFEFAQKFVAMEVLRDEEFSALKNADVAGKDCPSTARSDLHRLHKKYIETAGGIVHGDICEISPFITYAGENLASQVEGKSFTSPVYLRDTRDPLQGHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FC07</accession>
    <name>A0AAU9FC07_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Protein shuttle craft-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_11149</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NFX1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FC07"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="CDD" id="cd02643">
        <property type="entry name" value="R3H_NF-X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034078">
        <property type="entry name" value="NFX1_fam"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        </source>
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</entry>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <strain evidence="1 2">RF1</strain>
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        <source>
            <dbReference type="EMBL" id="BFF93164.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
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        <name type="scientific">Drosophila madeirensis</name>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    <feature type="transmembrane region" description="Helical" evidence="6">
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        <location>
            <begin position="302"/>
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        <location>
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        </location>
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        <location>
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            <end position="622"/>
        </location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1322"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
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            <end position="70"/>
        </location>
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        </location>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF93262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1358" mass="150161" checksum="D112C45E2A08F1FA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FC60</accession>
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        <submittedName>
            <fullName evidence="13">Zinc transporter 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_11154</name>
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    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FC60"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010043">
        <property type="term" value="P:response to zinc ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000040">
        <property type="entry name" value="Blast:Zinc transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000025">
        <property type="entry name" value="Zinc transporter 35C, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050681">
        <property type="entry name" value="CDF/SLC30A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562">
        <property type="entry name" value="CATION EFFLUX PROTEIN/ ZINC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562:SF17">
        <property type="entry name" value="RE54080P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="11">
        <location>
            <begin position="74"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="12">
        <location>
            <begin position="384"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="202"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF93272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="471" mass="50754" checksum="A70269FEB4A8891F" modified="2024-11-27" version="1">MSKNEDTPIAKGNYGTGQLQGQTNGGNGINNNPATPSTPSQIFCLHGRSNNVEVRDHCHRARSEGVDVRARQKLIIASVLCLVFMIAEIVGGYLSNSLAIATDAAHLLTDFASFMISLFAIWIAGRPSTQRMSFGWYRAEVIGAMASVFMIWVITGILVWLAIGRLITGDFDVDARIMLITSGLAILVNVIMGVQLQHGHSHTLGGGHGHSHGGGNSSKTSKKPKKSNKTGRLPSHIHATSTPCAASPTERIEGGVAFALEDAELPGSGLPTYSYQNAKLVDPTLDLEIAAVLAETAPGAHHHGGSVGREAVNMNVRAALIHVIGDVIQSVGVFVAALVIYFWPEYSVVDPICTFVFSIIVLFTTFTIMKDALLVLMEGTPNYMHYAEVLQIFQAIEGVERVHNLRIWALSINKVALSAHLAIGSNANPKQILDAATSAVHLRYNFFETTIQIEDYTAQMESCMQCNVPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FC61</accession>
    <name>A0AAU9FC61_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein rolling stone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_11124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FC61"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049352">
        <property type="entry name" value="Rost"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12242:SF46">
        <property type="entry name" value="IP08657P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12242">
        <property type="entry name" value="OS02G0130600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21534">
        <property type="entry name" value="Rost"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF93235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="164" mass="19338" checksum="582AD08CE9820BEF" modified="2024-11-27" version="1">MVKNSTKIKALWVCYWINMIALVYWTCIHTYVYPLNRDPTSPTYFTDLYKVWTHAMPPIFFHFDHLIVAQPTRLLHFLYPLEFVWIYTGFTLVYYVMGGLDLKGRRYTYNVLNYTNPREALLTIGLISASSVFVSTLQYVVYRFRTFLARSVIPELIFASTTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FC63</accession>
    <name>A0AAU9FC63_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_11146</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF51">
        <property type="entry name" value="ANOCTAMIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="507"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="650"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="701"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="726"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="8">
        <location>
            <begin position="302"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="599"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="777"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="957"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1057"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1289"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="605"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="808"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="971"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="987"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1064"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1097"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1126"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1289"/>
            <end position="1304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF93263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1325" mass="146312" checksum="8F5F2A4B2EB39DCC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKE1</accession>
    <name>A0AAU9FKE1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Galectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_13368</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKE1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016936">
        <property type="term" value="F:galactoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000180">
        <property type="entry name" value="Galectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044156">
        <property type="entry name" value="Galectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346:SF185">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00276">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Galectin" evidence="3">
        <location>
            <begin position="42"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Galectin" evidence="3">
        <location>
            <begin position="245"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF96105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="408" mass="46405" checksum="B55FE557D9C2E1D8" modified="2024-11-27" version="1">MMNICCGKRKRGANYSSDEEHQQHFLQDEASFYEYSDALPKYSNDQIGKLSEGVSFTITGSIAINCERFSINFVYANETRDVALHINPRLPQNYIVRNSKVADIWGSEEVSSALPFLLRRGDKFAIQVLITDACYMISVNGNHFAEYAHRIPYGAVQLLEVKGDVENVEMQRIVVENYPQRLPESQAKKIELHIVEGLDEIDANVEEAINIPHEWCLISAPVTHTDSSPKSTHSTTDAGLTLPYYGALPANSLFEGRCLKIEGRVRLLPHSFYINLQQGRDIWPHPVIAFHLNPRFSKASSGAIGKAVVCRNAWYNGSWAVEERSELDTNFRPGRTFSLAIVCAKESFEVYVNKQFITEFKYKVSPEIVDTVYIQGDVKLWNVTLEHNPKVKGKNMRIYHNPMMDDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKF2</accession>
    <name>A0AAU9FKF2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Potassium voltage-gated channel protein Shaw</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13479</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Shaw sub-subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKF2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032590">
        <property type="term" value="C:dendrite membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032809">
        <property type="term" value="C:neuronal cell body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030431">
        <property type="term" value="P:sleep"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18416">
        <property type="entry name" value="BTB_Shaw-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000020">
        <property type="entry name" value="Potassium voltage-gated channel protein Shaw"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000047">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003974">
        <property type="entry name" value="K_chnl_volt-dep_Kv3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF252">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN SHAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01498">
        <property type="entry name" value="SHAWCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="388"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="15">
        <location>
            <begin position="10"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="442"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="450"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF96238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="605" mass="66653" checksum="EB7784999EE4B6E0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKF8</accession>
    <name>A0AAU9FKF8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">U3 small nucleolar RNA-associated protein 14 homolog A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_05079</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UTP14 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKF8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:rRNA processing"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="SSU_processome_Utp14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14150">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14150:SF12">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 14 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Utp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="320"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="356"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF96416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="727" mass="83963" checksum="A13FBED88C32CABA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSP5</accession>
    <name>A0AAU9FSP5_DROMD</name>
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        <recommendedName>
            <fullName evidence="9">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_06692</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in the sugar gustatory response.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family. Gr5a subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:sweet taste receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Gustatory_rcpt"/>
    </dbReference>
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        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21421:SF29">
        <property type="entry name" value="GUSTATORY RECEPTOR 5A FOR TREHALOSE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Trehalose_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF98553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="429" mass="49484" checksum="9F11D5D87BFBC520" modified="2024-11-27" version="1">MARTMGDPAKRYKFVARIKFWRRSRVGSDITLGVLKYKMTSKQPQHFQFAKIKAFMRRALKDNYNYSGTFQEAIKPVLIIAQIFALMPVRGIGSKFAEDLTFAWSSFRTYYALAMMISFGITAGYIVAFLTNVSFDFDSVETMMFYGSIFLISISFLQLARRWPAIAQEWQAVEAKLPPLRLAKERRSLAHHIKMITLVATTCSLVEHLLSMTSTVTFAVACPHWPAHPIDNFLYFNFATVFHFVEYTTFLGLLGKVINVLSTFAWNFNDIFVMAVSVALASRFRQLNDYMQREARTATTVEYWMHCRINFRNLCKLCQVVDDGISAITLLCFSNNLYFICGKILKSMHSSINDESKRPLRIFRLVPREYWCDELKRFSEEVHMDTVALTGMKFFRLTRGVVISVAGTIVTYELILLQFNKEETTAFTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSQ2</accession>
    <name>A0AAU9FSQ2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">LIM/homeobox protein Awh</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_06659</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007444">
        <property type="term" value="P:imaginal disc development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09373">
        <property type="entry name" value="LIM1_AWH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09379">
        <property type="entry name" value="LIM2_AWH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000023">
        <property type="entry name" value="LIM homeobox 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000006">
        <property type="entry name" value="LIM homeobox transcription factor 1-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000027">
        <property type="entry name" value="LIM/homeobox protein Lhx9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF127">
        <property type="entry name" value="LIM_HOMEOBOX PROTEIN AWH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 12" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="6"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="68"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="146"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="148"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="235"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF98504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="271" mass="30613" checksum="1EE4EAEA0B7EECFB" modified="2024-11-27" version="1">MKTELRSCAACGEPISDRFFLEVGGCSWHAHCLRCCMCMCPLDRQQSCFIRERQVYCKADYSKNFGAKCSKCCRGISASDWVRRARDLVFHLACFACDQCGRQLSTGEQFALMDDRVLCKAHYLETVEGGTTSSDEGCDGDGYHKSKTKRVRTTFTEEQLQVLQANFQIDSNPDGQDLERIASVTGLSKRVTQVWFQNSRARQKKHIHAVREPEGSSFARHINLQLTYSFQNNAQNPMHMNGSKAGLYPTHESSMDEMSQDSSVHCMPSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSU7</accession>
    <name>A0AAU9FSU7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="9">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_06775</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="9"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FSU7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019871">
        <property type="term" value="F:sodium channel inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008586">
        <property type="term" value="P:imaginal disc-derived wing vein morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045879">
        <property type="term" value="P:negative regulation of smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048260">
        <property type="term" value="P:positive regulation of receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048814">
        <property type="term" value="P:regulation of dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04033">
        <property type="entry name" value="C2_NEDD4_NEDD4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000037">
        <property type="entry name" value="E3 ubiquitin-protein ligase nedd-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000096">
        <property type="entry name" value="E3 ubiquitin-protein ligase Nedd-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000021">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024928">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF440">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEDD-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001569">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 9 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="52"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="247"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="356"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="407"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="15">
        <location>
            <begin position="499"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="208"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="304"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="385"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="313"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10 11">
        <location>
            <position position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001569-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF98685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="834" mass="94989" checksum="3CD4A50706DFABCF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSV2</accession>
    <name>A0AAU9FSV2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glucose transporter type 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_06672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FSV2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="141"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF98526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSW4</accession>
    <name>A0AAU9FSW4_DROMD</name>
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            <fullName evidence="25">Membrane-bound transcription factor site-1 protease</fullName>
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        <alternativeName>
            <fullName evidence="26">Endopeptidase S1P</fullName>
        </alternativeName>
    </protein>
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        <name evidence="35" type="ORF">DMAD_06807</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="35 36" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="35 36">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="23">
            <text>Processes precursors containing basic and hydrophobic/aliphatic residues at P4 and P2, respectively, with a relatively relaxed acceptance of amino acids at P1 and P3.</text>
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    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 27">Belongs to the peptidase S8 family.</text>
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        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="14" id="KW-0106">Calcium</keyword>
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    <keyword evidence="28" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 27" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="18 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 27 35" id="KW-0645">Protease</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 27" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9 30" id="KW-0732">Signal</keyword>
    <keyword evidence="22" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="20" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="8 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 29" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="19" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="30">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043403810" description="Membrane-bound transcription factor site-1 protease" evidence="30">
        <location>
            <begin position="28"/>
            <end position="972"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="944"/>
            <end position="966"/>
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    <feature type="domain" description="Membrane-bound transcription factor site-1 protease-like N-terminal" evidence="32">
        <location>
            <begin position="37"/>
            <end position="120"/>
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    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="31">
        <location>
            <begin position="168"/>
            <end position="424"/>
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    </feature>
    <feature type="domain" description="MBTPS1 third" evidence="34">
        <location>
            <begin position="445"/>
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    </feature>
    <feature type="domain" description="MBTPS1 fourth" evidence="33">
        <location>
            <begin position="573"/>
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    </feature>
    <feature type="coiled-coil region" evidence="28">
        <location>
            <begin position="900"/>
            <end position="927"/>
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    </feature>
    <feature type="active site" description="Charge relay system" evidence="27">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="27">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="27">
        <location>
            <position position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23094"/>
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        <source>
            <dbReference type="EMBL" id="BFF98727.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
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    <sequence length="972" mass="109061" checksum="4C25CF54BC637CBE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FT01</accession>
    <name>A0AAU9FT01_DROMD</name>
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        <submittedName>
            <fullName evidence="14">Protein LSM14 homolog B-A</fullName>
        </submittedName>
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        <name evidence="14" type="ORF">DMAD_06866</name>
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    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">As a component of the decapping complex, involved in the degradation of mRNAs. Promotes P-body formation. Translational repressor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LSM14 family.</text>
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        <property type="protein sequence ID" value="BFF98812.1"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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    </feature>
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        <location>
            <begin position="514"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="132"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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        <location>
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    </feature>
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            <begin position="294"/>
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        </location>
    </feature>
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        <location>
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        </location>
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    </feature>
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        <location>
            <begin position="586"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010415"/>
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    <evidence type="ECO:0000256" key="3">
        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00059323"/>
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        <source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00869"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51512"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51536"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS52002"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF98812.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="670" mass="71227" checksum="B28F6CBB797692A8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FT04</accession>
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        <recommendedName>
            <fullName evidence="5">Zinc finger CCCH domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
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        <name evidence="9" type="ORF">DMAD_06736</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="CCCH ZINGC FINGER"/>
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    </dbReference>
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        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C3H1"/>
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    </dbReference>
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        <property type="entry name" value="ZF_C3H1"/>
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    </dbReference>
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    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
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    </feature>
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        <location>
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    </feature>
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    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
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    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="506"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
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            <end position="515"/>
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            <begin position="566"/>
            <end position="577"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF98611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="601" mass="66879" checksum="7CF93CF8A6A57FD5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FT09</accession>
    <name>A0AAU9FT09_DROMD</name>
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        <submittedName>
            <fullName evidence="7">Serine protease nudel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_07003</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome J"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="LDLa"/>
        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Blast:Serine protease nudel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000201">
        <property type="entry name" value="Serine protease nudel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000210">
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    </dbReference>
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        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015420">
        <property type="entry name" value="Peptidase_S1A_nudel"/>
    </dbReference>
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        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
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        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF1986"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00089">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="8"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <location>
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            <end position="47"/>
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            <end position="2188"/>
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        </location>
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            <begin position="756"/>
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        </location>
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        <location>
            <begin position="1513"/>
            <end position="1591"/>
        </location>
    </feature>
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        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
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        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
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            <end position="294"/>
        </location>
    </feature>
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    </feature>
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        </location>
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    </feature>
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    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="2329"/>
            <end position="2344"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF98993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2503" mass="278737" checksum="DEA30748815F1784" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FT10</accession>
    <name>A0AAU9FT10_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ras-related protein Rab-26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_07147</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="75"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="75"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="385" mass="42814" checksum="318A1CE49B1CC9EB" modified="2024-11-27" version="1">MATTAATGITAAGTGTTGTTGTHHHDDASSMSDDVFEDAETTQARIEELRRRPFGDGCYNPPAAPVSISATVAASASAATTTQQQQQQQQHHHHHHHQYPNHHQSQQSLTGSSHHYHDDAIMAPVQRTATGYPGYRPSREALQMYAYGTGDDYDDYDDGWRSYRYDEVDMHQTQPTANLQPFDDTVNHKTILLGDSGVGKTSFLVKYNTGEFRLGSFSATVGIALTNKVVVVDGTRVKLQIWDTAGQERFRSVTHAYYRDAHALLLLYDVTNKTTYDNIRAWLGEIREYAQEDVVIVLIGNKADCSGSERQVKREDGERLGREHNVPFMETSAKTGLNVELAFTAVARQLKSRGYDHGDDGKFNVHDFVRDNTKARSVCAQCRNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="ORF">DMAD_06951</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF98923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="27090" checksum="9CE2EDDA041A35E3" modified="2024-11-27" version="1">MDFQTKYSKRHLNGTIYIATRRVLEEKLRSELASGAGELYLSFKAPFAAYDIEQIAQVASELGDIYVIRFKVDFRSESRGYAFLQYIDASLQAKAMEVLKQRFRDINLDINILPSRNCRELVMITAAGTGPTPLKVYQQMLKICSFNSVRVYEYRPHTYMHIFSYVNNEAATLAFRDIRSNILRFGHGAHVSWLRTNQTLANDDFVTDCCQQLDSEMKPESNAGTCNCFTFSRPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FT19</accession>
    <name>A0AAU9FT19_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Arginine-glutamic acid dipeptide repeats protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_06738</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000052">
        <property type="entry name" value="Arginine-glutamic acid dipeptide (RE) repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="arginine-glutamic acid dipeptide repeats protein-like"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000949">
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    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13859">
        <property type="entry name" value="ATROPHIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13859:SF11">
        <property type="entry name" value="GRUNGE, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01189">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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            <begin position="7"/>
            <end position="118"/>
        </location>
    </feature>
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        </location>
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    </feature>
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        </location>
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        </location>
    </feature>
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        </location>
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        </location>
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            <begin position="1839"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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        </location>
    </feature>
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            <end position="1384"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
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        </location>
    </feature>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF98626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2067" mass="220413" checksum="0076AB43FC86582B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FT23</accession>
    <name>A0AAU9FT23_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">adenylate cyclase</fullName>
            <ecNumber evidence="4">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_07119</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
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            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
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    <dbReference type="EMBL" id="AP029265">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FT66</accession>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Arthropoda</taxon>
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            <taxon>Endopterygota</taxon>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6 7">RF1</strain>
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    <keyword evidence="3" id="KW-0862">Zinc</keyword>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF98832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="702" mass="77726" checksum="9EB11EC92C297717" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FT70</accession>
    <name>A0AAU9FT70_DROMD</name>
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        <recommendedName>
            <fullName evidence="14">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="14">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_06923</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">E3 ubiquitin-protein ligase that is required for specification of R7 photoreceptor cell fate in the eye by mediating the ubiquitination and subsequent proteasomal degradation of Tramtrack (ttk). E3 Ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Acts via the formation of a complex with ebi and phyl that ubiquitinates the transcription repressor ttk, a general inhibitor of photoreceptor differentiation, in a subset of photoreceptor cells in the eye, leading to the differentiation of cells into neurons. Also involved in external sensory organ development.</text>
    </comment>
    <comment type="function">
        <text evidence="14">E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of some E3 complex at least composed of sina, ebi and phyl. Interacts with eff.</text>
    </comment>
    <comment type="domain">
        <text evidence="14">The RING-type zinc finger domain is essential for ubiquitin ligase activity.</text>
    </comment>
    <comment type="domain">
        <text evidence="14">The SBD domain (substrate-binding domain) mediates the interaction with substrate proteins. It is related to the TRAF family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the SINA (Seven in absentia) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="14"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FT70"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03829">
        <property type="entry name" value="Sina"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000002">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000041">
        <property type="entry name" value="E3 ubiquitin-protein ligase SINAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018121">
        <property type="entry name" value="7-in-absentia-prot_TRAF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004162">
        <property type="entry name" value="SINA-like_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45877">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SIAH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45877:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SINA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03145">
        <property type="entry name" value="Sina_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21361">
        <property type="entry name" value="Sina_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0844">Vision</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SIAH-type" evidence="15">
        <location>
            <begin position="7"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00062579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU201113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF98887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="196" mass="22058" checksum="A705298604032FD8" modified="2024-11-27" version="1">MEKVASNVKFPCKHSGYGCTASLVYTEKTEHEETCECRPYLCPCPGASCKWQGPLDLVMQHLMMSHKSITTLQGEDIVFLATDINLPGAVDWVMMQSCFGHHFMLVLEKQEKYDGHQQFFAIVQLIGSRKEAENFVYRLELNGNRRRLTWEAMPRSIHEGVASAIHNSDCLVFDTSIAQLFADNGNLGINVTISLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FT79</accession>
    <name>A0AAU9FT79_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">HECT-type E3 ubiquitin transferase NEDD4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_06775</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FT79"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019871">
        <property type="term" value="F:sodium channel inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008586">
        <property type="term" value="P:imaginal disc-derived wing vein morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045879">
        <property type="term" value="P:negative regulation of smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048260">
        <property type="term" value="P:positive regulation of receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048814">
        <property type="term" value="P:regulation of dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04033">
        <property type="entry name" value="C2_NEDD4_NEDD4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000037">
        <property type="entry name" value="E3 ubiquitin-protein ligase nedd-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000096">
        <property type="entry name" value="E3 ubiquitin-protein ligase Nedd-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000021">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024928">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF440">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEDD-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001569">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="52"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="15">
        <location>
            <begin position="246"/>
            <end position="279"/>
        </location>
    </feature>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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                <person name="Yamamoto D."/>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <protein>
        <submittedName>
            <fullName evidence="9">Cytoplasmic dynein 1 intermediate chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_02105</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G4P4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000552">
        <property type="entry name" value="Cytoplasmic dynein 1 intermediate chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001109">
        <property type="entry name" value="Cytoplasmic dynein 1 intermediate chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025956">
        <property type="entry name" value="DYNC1I1/DYNC1I2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF22">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 INTERMEDIATE CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11540">
        <property type="entry name" value="Dynein_IC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="496"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="17"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG02652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="636" mass="71092" checksum="D2D5C526AC7EC879" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4Q2</accession>
    <name>A0AAU9G4Q2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Neogenin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_02711</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. DCC family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000004">
        <property type="entry name" value="DCC isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002650">
        <property type="entry name" value="Frazzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001472">
        <property type="entry name" value="Frazzled, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001583">
        <property type="entry name" value="Frazzled, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002008">
        <property type="entry name" value="Frazzled, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001448">
        <property type="entry name" value="Frazzled, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001797">
        <property type="entry name" value="Frazzled, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000028">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010560">
        <property type="entry name" value="Neogenin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF54">
        <property type="entry name" value="FI24025P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06583">
        <property type="entry name" value="Neogenin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043953185" evidence="12">
        <location>
            <begin position="27"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1257"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="23"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="127"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="244"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="337"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="642"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="741"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="836"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="936"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="1039"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="1138"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="425"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="502"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="578"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1405"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1492"/>
            <end position="1517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="428"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="449"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="513"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="538"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="586"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1409"/>
            <end position="1424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG02761.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1452" mass="156757" checksum="830EBD91D999C624" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G501</accession>
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        <submittedName>
            <fullName evidence="14">Transient receptor potential cation channel trpm</fullName>
        </submittedName>
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        <name evidence="14" type="ORF">DMAD_02501</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="BFG03184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050927">
        <property type="entry name" value="TRPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057366">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TRPM_tetra_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800:SF1">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL TRPM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL, SUBFAMILY M, MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LSDAT_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRPM_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
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    <keyword evidence="14" id="KW-0675">Receptor</keyword>
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    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="900"/>
            <end position="918"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="967"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1047"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="domain" description="TRPM SLOG" evidence="12">
        <location>
            <begin position="116"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="TRPM-like" evidence="13">
        <location>
            <begin position="441"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="10">
        <location>
            <begin position="841"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="domain" description="TRPM tetramerisation" evidence="11">
        <location>
            <begin position="1163"/>
            <end position="1218"/>
        </location>
    </feature>
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            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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            <end position="1413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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            <end position="1490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="1683"/>
            <end position="1709"/>
        </location>
    </feature>
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            <begin position="1781"/>
            <end position="1814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1873"/>
            <end position="1914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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        </location>
    </feature>
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            <begin position="1696"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1873"/>
            <end position="1888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1897"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF18139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFG03184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1947" mass="217412" checksum="948A5D177F3EBB61" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G502</accession>
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        <submittedName>
            <fullName evidence="13">Proton channel OtopLc</fullName>
        </submittedName>
    </protein>
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        <name evidence="13" type="ORF">DMAD_02176</name>
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    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the otopetrin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004878">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522:SF62">
        <property type="entry name" value="OTOPETRIN-LIKE A, ISOFORM C"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522">
        <property type="entry name" value="PROTON CHANNEL OTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03189">
        <property type="entry name" value="Otopetrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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            <end position="120"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
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        <location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="17"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
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    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG02757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G505</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name evidence="2" type="ORF">DMAD_02095</name>
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            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EMBL" id="BFG02630.1"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <subcellularLocation>
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        </location>
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        <location>
            <begin position="1415"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1475"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1883"/>
            <end position="1915"/>
        </location>
    </feature>
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        <location>
            <begin position="1916"/>
            <end position="1925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1977"/>
            <end position="1991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="2007"/>
            <end position="2018"/>
        </location>
    </feature>
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        <location>
            <begin position="2019"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="2048"/>
            <end position="2072"/>
        </location>
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    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="2076"/>
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        <location>
            <begin position="2103"/>
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        <location>
            <begin position="2137"/>
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        <source>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <sequence length="2169" mass="235511" checksum="CAE6462EDA0D17AE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G511</accession>
    <name>A0AAU9G511_DROMD</name>
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        <submittedName>
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        </submittedName>
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        <name evidence="7" type="ORF">DMAD_02818</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
        </source>
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        </source>
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        </source>
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    <sequence length="499" mass="54694" checksum="10564C14735AA1B4" modified="2024-11-27" version="1">MPYNGASNGSGGAGGGGGGATIVVSEGPQNKKIRTGVQQPGENDVHMHARSTQQQNQQQALMNKSNDDLRRKRPETTRPNHILLFTIINPFYPITVDVLHKICHPHGQVLRIVIFKKNGVQAMVEFDNLDAATRARENLNGADIYAGCCTLKIDYAKPEKLNVYKNEPDTSWDYTLSTVKDIGNGRSPLLQEPLYGTRPQPYKPPLLGPGAAFPPFGAPEYHPTTPDNWKGASIHPTGLMKEPTGVVPGRNAPVAFTPQGQAQGAVMMVYGLDHDTSNTDKLFNLVCLYGNVARIKFLKTKEGTAMVQMGDSVAVERCVQHLNNIPVGTGGKIQIAFSKQNFLSEVINPFLLPDHTPSFKEYTGSKNNRFLSPAQASKNRIQPPSKILHFFNTPPGLTEDQLIGIFNIKDVPATSVRLFPLKTERSSSGLIEFPNISQAVLAIMKCNHLPIEGKGTKFPFIMKLCFSSSKSMNGAWNNATNEGMIEKENEVDSKVDIYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G514</accession>
    <name>A0AAU9G514_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Glutathione synthetase</fullName>
            <shortName evidence="13">GSH-S</shortName>
            <ecNumber evidence="4 13">6.3.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_02371</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13557"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13558"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13 15">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the eukaryotic GSH synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.3" evidence="4 13"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG03018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004363">
        <property type="term" value="F:glutathione synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutathione synthetase"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1760">
        <property type="entry name" value="Glutathione synthase, substrate-binding domain superfamily, eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1080.10">
        <property type="entry name" value="Glutathione Synthetase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005615">
        <property type="entry name" value="Glutathione_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014042">
        <property type="entry name" value="Glutathione_synthase_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014709">
        <property type="entry name" value="Glutathione_synthase_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014049">
        <property type="entry name" value="Glutathione_synthase_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037013">
        <property type="entry name" value="GSH-S_sub-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004887">
        <property type="entry name" value="GSH_synth_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01986">
        <property type="entry name" value="glut_syn_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130:SF0">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03917">
        <property type="entry name" value="GSH_synth_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03199">
        <property type="entry name" value="GSH_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001558">
        <property type="entry name" value="GSHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13" id="KW-0317">Glutathione biosynthesis</keyword>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G518</accession>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G524</accession>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <strain evidence="19 20">RF1</strain>
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        <subcellularLocation>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Jelly Rolls"/>
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        <property type="entry name" value="KCNH_animal-type"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="365"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="437"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="466"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="17">
        <location>
            <begin position="43"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="18">
        <location>
            <begin position="111"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="16">
        <location>
            <begin position="592"/>
            <end position="669"/>
        </location>
    </feature>
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        <location>
            <begin position="735"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="835"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="878"/>
            <end position="910"/>
        </location>
    </feature>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <dbReference type="EMBL" id="BFG03214.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19529" checksum="C3BF91DB50D53FFF" modified="2024-11-27" version="1">MTNPIEQRKWDYEPVSITTHSSDESMLKIEAKIDRVKRGEFAISATIDFNYAIDDTVMIEAVAYRSSTGQESDYKLVPFNIQKQTYEEFLNTHYKDMVIQNLGSCSDLPQFEDKFVPPWPQKTYKLDKCVADNDGFPEVLPLGFYKIVLSITGPVEWGITIITKLTNKLM</sequence>
</entry>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G571</accession>
    <name>A0AAU9G571_DROMD</name>
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        <submittedName>
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    </protein>
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    </gene>
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        <lineage>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
    </comment>
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        <text evidence="3">Belongs to the CARMIL family.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Leucine-rich repeat, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031943">
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    </dbReference>
    <dbReference type="InterPro" id="IPR041245">
        <property type="entry name" value="CARMIL_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051279">
        <property type="entry name" value="PP1-Reg/Actin-Interact_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112:SF66">
        <property type="entry name" value="LEUCINE-RICH REPEAT, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17888">
        <property type="entry name" value="Carm_PH"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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            <begin position="25"/>
            <end position="123"/>
        </location>
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            <begin position="779"/>
            <end position="1086"/>
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        <location>
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            <begin position="1060"/>
            <end position="1082"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="1156"/>
            <end position="1179"/>
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        <location>
            <begin position="1204"/>
            <end position="1231"/>
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    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="1305"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="875"/>
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        <source>
            <dbReference type="Pfam" id="PF17888"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03640.1"/>
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    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G576</accession>
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        <submittedName>
            <fullName evidence="15">Kinesin-like protein KIF13A</fullName>
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        <name evidence="15" type="ORF">DMAD_02593</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
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    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0007018">
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
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        <property type="entry name" value="Kinesin_motor_CS"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CAP_GLY_1"/>
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        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="5"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="14">
        <location>
            <begin position="1848"/>
            <end position="1890"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="829"/>
            <end position="853"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
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        <location>
            <begin position="1755"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1905"/>
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        <location>
            <begin position="589"/>
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        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1648"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1692"/>
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    </feature>
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        <location>
            <begin position="1715"/>
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    </feature>
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        <location>
            <begin position="1764"/>
            <end position="1779"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1912"/>
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    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="100"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50067"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50245"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03299.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1939" mass="217274" checksum="FB87AF6CC4314527" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G578</accession>
    <name>A0AAU9G578_DROMD</name>
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        <submittedName>
            <fullName evidence="20">Potassium voltage-gated channel protein eag</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_02225</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
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        <location>
            <begin position="85"/>
            <end position="185"/>
        </location>
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        <location>
            <begin position="32"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="503"/>
            <end position="637"/>
        </location>
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        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
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        <location>
            <begin position="507"/>
            <end position="516"/>
        </location>
    </feature>
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        <location>
            <begin position="547"/>
            <end position="561"/>
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            <begin position="562"/>
            <end position="574"/>
        </location>
    </feature>
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        <location>
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            <end position="604"/>
        </location>
    </feature>
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        <location>
            <begin position="612"/>
            <end position="637"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
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            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG02705.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="655" mass="73453" checksum="8E567BDDD9FA2E28" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G584</accession>
    <name>A0AAU9G584_DROMD</name>
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        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="ORF">DMAD_02451</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of tube size, open tracheal system"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019991">
        <property type="term" value="P:septate junction assembly"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
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        <property type="entry name" value="EG:80H7.2 PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="KUNE-KUNE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
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        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="236" mass="26763" checksum="78ECB3E2D290E448" modified="2024-11-27" version="1">MGASNRNGRLEKPRFTNLGLWEVCFNDFQDIHRFYDNPFNGCMWVFEEEYYIIHDFLLPGFYIAVQLFATLCFVMCLIGLPLTLAFLRTSRDDDRYVVLLLTIGTCQVLGSIFGFIAVVIFGAKGDSRDWMPGWQNNDMGWSFALGVVGAVLLLPGGILYMVEARRERYKRLNEISNREVSEYGDDCYQHQANVATTPTAPSPSQSYFAPEPSRPRRPQSGRTPNIPPHGGIQTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G586</accession>
    <name>A0AAU9G586_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_02432</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
            <location evidence="1 10">Extracellular space</location>
            <location evidence="1 10">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G586"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060560">
        <property type="term" value="P:developmental growth involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Wnt_Wnt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF77">
        <property type="entry name" value="PROTEIN WNT-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="465" mass="52697" checksum="3BFE964FCF32D5A3" modified="2024-11-27" version="1">MPAISRGARAAIQECQFQFKSRRWNCSTTDDETVFGPMTILAAPEMAFIHALAAATVTSFVARACRDGQLASCGCSRGNRPKQLNDDWTWGGCGDNLEYAYKFATDFIDVREKEAHRETRGVGKRAREFTQSQMQRTQMQADDIKTFGTSTLKMSNLTSLIDAKNGTVENVSRELRKKPSGKNNYFRNDLVVTATPLKLKKKEDSGLGLPDDLFELQQRITKEILNSKLEGREMLVLQEKINREILNSKIFQADDKSNRRKRKRKNQRAVVEDAPSAGNIDRGNYVDGVDTRTATNTKARSLMNLHNNEAGRRAVIKKTRITCKCHGVSGSCSLITCWQQLSSIREIGDYLREKFEEATKVKLNKRGRLQVKDSQFKVPTAHDLIYIDESPDWCRNNYILHWPGTHGRVCQKSSSGLDSCAILCCGRGYNTKNIVVHERCNCKFHWCCQVKCEVCTKVQEEHTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEQ6</accession>
    <name>A0AAU9GEQ6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="16 17">RF1</strain>
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        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
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        <property type="project" value="UniProtKB-ARBA"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEQ8</accession>
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <end position="1172"/>
        </location>
    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG06011.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GER1</accession>
    <name>A0AAU9GER1_DROMD</name>
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        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="10" type="ORF">DMAD_04549</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
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        </reaction>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
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    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
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        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="128"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="318"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="358"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG05934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="457" mass="51557" checksum="052D3075B1FED0A3" modified="2024-11-27" version="1">MGNDDLRRRKTPCGFCMAVFKWIPVVFITAVIAWSYYAYVVELCIRNAENLVGMIFMLFFYHIALILFMWSYWRTIMTSVGRVPDQWRIPDEEVTRLFRADNPETQKRILNSFARSLPVTNRTMNGSVRFCEKCKIIKPDRAHHCSVCNSCVLKMDHHCPWVNNCVNFYNYKFFVLFLGYALVYCLYVAFTTLHDFVQFWKGQLNGSGVGRFHILFLFFISIMFAISLVSLFGYHIYLVLVNRTTLESFRAPVFRVGGPDKNGFNLGRYANFCEVFGDDWQYWPLPIFTSRGDGFSYPTSTDQSGEAPTVQRYAAMGDTTTNRLDGNPTDKLIDAIPLDTTPNNHDQPPPQHQHPHQVRSQNQAQPQPQQARHQARPNSLNLQPALELPLANGQSSNCSTAAVPSSQMVVVNLGTLPEDANGSAVIIDMVGDHSGAAAGVAAPKDACRQPNGDVPLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GER8</accession>
    <name>A0AAU9GER8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Bone morphogenetic protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_13650</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GER8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13755">
        <property type="entry name" value="TGF_beta_maverick"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000058">
        <property type="entry name" value="Maverick"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF119">
        <property type="entry name" value="TGF-BETA FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0339">Growth factor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="7">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFG06734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="119" mass="13982" checksum="77AB4BC75483C6FF" modified="2024-11-27" version="1">MGWRREKWNNSCYKVHQRCCRHQLDVVFKDIKGFEFILQPKMFDAGYCHGRCPPRHNPAHHHALLQSLIWQQDSSRAPRPCCAPSKLKVLEILHVDEEHTDKLKISTWSDMQVLECACS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GER9</accession>
    <name>A0AAU9GER9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Beta-glucuronidase</fullName>
            <ecNumber evidence="5 12">3.2.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_05032</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Plays an important role in the degradation of dermatan and keratan sulfates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a beta-D-glucuronoside + H2O = D-glucuronate + an alcohol</text>
            <dbReference type="Rhea" id="RHEA:17633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:58720"/>
            <dbReference type="ChEBI" id="CHEBI:83411"/>
            <dbReference type="EC" id="3.2.1.31"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="12">Inhibited by L-aspartic acid.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.31" evidence="5 12"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GER9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004566">
        <property type="term" value="F:beta-glucuronidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019391">
        <property type="term" value="P:glucuronoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000027">
        <property type="entry name" value="Beta-glucuronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000628">
        <property type="entry name" value="Beta-glucuronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000029">
        <property type="entry name" value="Beta-glucuronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006101">
        <property type="entry name" value="Glyco_hydro_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023232">
        <property type="entry name" value="Glyco_hydro_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006103">
        <property type="entry name" value="Glyco_hydro_2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023230">
        <property type="entry name" value="Glyco_hydro_2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006104">
        <property type="entry name" value="Glyco_hydro_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006102">
        <property type="entry name" value="Ig-like_GH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007538">
        <property type="entry name" value="PRK10150.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10066">
        <property type="entry name" value="BETA-GLUCURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10066:SF67">
        <property type="entry name" value="BETA-GLUCURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00703">
        <property type="entry name" value="Glyco_hydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02836">
        <property type="entry name" value="Glyco_hydro_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02837">
        <property type="entry name" value="Glyco_hydro_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00132">
        <property type="entry name" value="GLHYDRLASE2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="beta-Galactosidase/glucuronidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00719">
        <property type="entry name" value="GLYCOSYL_HYDROL_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00608">
        <property type="entry name" value="GLYCOSYL_HYDROL_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolases family 2 sugar binding" evidence="16">
        <location>
            <begin position="59"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 2 immunoglobulin-like beta-sandwich" evidence="14">
        <location>
            <begin position="238"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 2 catalytic" evidence="15">
        <location>
            <begin position="354"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG06543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="674" mass="77780" checksum="A52B852D46856543" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GES2</accession>
    <name>A0AAU9GES2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14114">
        <property type="entry name" value="STKc_Twitchin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000440">
        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="4"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:000642">
        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform H"/>
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        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000147">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myosin-binding protein C, slow type"/>
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    </dbReference>
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        <property type="entry name" value="Protein CBR-UNC-22"/>
        <property type="match status" value="4"/>
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        <property type="entry name" value="Titin a"/>
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        <property type="entry name" value="Titin isoform E"/>
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        <property type="entry name" value="titin isoform X1"/>
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        <property type="entry name" value="titin isoform X1"/>
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        <property type="entry name" value="titin isoform X1"/>
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        <property type="entry name" value="twitchin isoform X2"/>
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        <property type="entry name" value="twitchin isoform X4"/>
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        <property type="entry name" value="Uncharacterized protein, isoform C"/>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
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        <property type="entry name" value="Uncharacterized protein, isoform G"/>
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        <property type="entry name" value="Uncharacterized protein, isoform G"/>
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        <property type="entry name" value="Uncharacterized protein, isoform H"/>
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        <property type="entry name" value="Uncharacterized protein, isoform H"/>
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        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform I"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform I"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000553">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="76"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
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    </dbReference>
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        <property type="entry name" value="FN3_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF151">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
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    </dbReference>
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        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="40"/>
    </dbReference>
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        <property type="match status" value="36"/>
    </dbReference>
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        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="37"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="9"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="119"/>
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    <feature type="domain" description="Ig-like" evidence="14">
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            <begin position="223"/>
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    <feature type="domain" description="Ig-like" evidence="14">
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    <feature type="domain" description="Ig-like" evidence="14">
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    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="1533"/>
            <end position="1617"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="1811"/>
            <end position="1908"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="1911"/>
            <end position="2009"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="2013"/>
            <end position="2103"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="2110"/>
            <end position="2206"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="2212"/>
            <end position="2307"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="2311"/>
            <end position="2401"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="2409"/>
            <end position="2505"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="2512"/>
            <end position="2607"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="2611"/>
            <end position="2703"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="2707"/>
            <end position="2806"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="2812"/>
            <end position="2907"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="2911"/>
            <end position="3000"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="3008"/>
            <end position="3102"/>
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    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="3109"/>
            <end position="3204"/>
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    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG06523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="498" mass="51907" checksum="6FE0298A1DA52871" modified="2024-11-27" version="1">MPHTGQAGINQLGGVFVNGRPLPDCTRLRIVELALCGVRPCDISRQLLVSHGCVSKILTRYHETGSIRPGSIGGSKTKQVATPTVVKKITRLKEENSGMFAWEIREQLQAQRVCDPSSVPSISSINRILRHSGLWTDEMTSSQQNAAAAAAAHQAGAGGPGGPGAGYATPSQLYGLPSNNNNSSHSPKTQAAAGTNGPPTATAPPTGGRYAKPPAMMMTPTGGEMPLKPAPKLPSSHGHGQHSHGHGHGHGHGHGHGHGHGHGLSASVSGGGGGGGMSVPSQLGGLDLSYSTLHKHWLWNPSLLYYTQAHMQAAATGAQFLPYGGGYLPHGMAAAAAAAVASECTNGGGFTKSESSIDLSTPGATGDALSDCDSGKSSPAALSLNAARGQLLSHSHGHSHSRKRNPYSIEELLKKPEKRLRLSSESQASLMEGKRRSEHLECLSSSSCESSQDGSNGDVPLPVDTVEEVVPVDEEDEQQQQQQEEEEEEEDCSVEVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GES7</accession>
    <name>A0AAU9GES7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">protein kinase C</fullName>
            <ecNumber evidence="7">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">DMAD_04829</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30 31">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="7"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11622">
        <property type="entry name" value="HR1_PKN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11623">
        <property type="entry name" value="HR1_PKN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11625">
        <property type="entry name" value="HR1_PKN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05589">
        <property type="entry name" value="STKc_PKN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000001">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000002">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000058">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000008">
        <property type="entry name" value="serine/threonine-protein kinase N isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000038">
        <property type="entry name" value="serine/threonine-protein kinase N2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.160">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037313">
        <property type="entry name" value="PKN_HR1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02185">
        <property type="entry name" value="HR1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17 23 25" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 30" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="19" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="REM-1" evidence="29">
        <location>
            <begin position="124"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="29">
        <location>
            <begin position="228"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="29">
        <location>
            <begin position="324"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="1239"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="28">
        <location>
            <begin position="1499"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="476"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="654"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="911"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1112"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1185"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="835"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="26">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="476"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="663"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="920"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="26">
        <location>
            <begin position="942"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="955"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="984"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="1112"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="1185"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1268"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="BFG06291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1566" mass="174103" checksum="6DFA9D91F7229734" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEW0</accession>
    <name>A0AAU9GEW0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_13674</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the synaptopodin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GEW0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032233">
        <property type="term" value="P:positive regulation of actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051976">
        <property type="entry name" value="Synaptopodin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24217:SF0">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24217">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="110"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="327"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="862"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="263"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="217"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="339"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="865"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="883"/>
            <end position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG06779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="942" mass="104597" checksum="426EE6E5B7E890E6" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEW3</accession>
    <name>A0AAU9GEW3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_04596</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing (1-&gt;4)-linked alpha-D-glucose residues with release of alpha-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.20"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.20" evidence="3"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GEW3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11328">
        <property type="entry name" value="AmyAc_maltase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.400.10:FF:000001">
        <property type="entry name" value="Maltase A3, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="entry name" value="Oligo-1,6-glucosidase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF234">
        <property type="entry name" value="MALTASE A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="7">
        <location>
            <begin position="1"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFG05994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="541" mass="60921" checksum="2DD76FBB075085F6" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEW5</accession>
    <name>A0AAU9GEW5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase C-like 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05038</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GEW5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20831">
        <property type="entry name" value="C1_dGM13116p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08678">
        <property type="entry name" value="C2_C21orf25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039934">
        <property type="entry name" value="C2CD2/C2CD2L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21119">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21119:SF5">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="5">
        <location>
            <begin position="358"/>
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        </location>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Serine/threonine kinase that plays a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="9"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GEX6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008349">
        <property type="term" value="F:MAP kinase kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06613">
        <property type="entry name" value="STKc_MAP4K3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000031">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021160">
        <property type="entry name" value="MAPKKKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF18">
        <property type="entry name" value="HAPPYHOUR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038172">
        <property type="entry name" value="MAPKKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="26"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="15">
        <location>
            <begin position="580"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="454"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="504"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="454"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="506"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="32"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038172-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038172-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG06583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="939" mass="104234" checksum="18E86E72840E2532" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GF22</accession>
    <name>A0AAU9GF22_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(+) kinase</fullName>
            <ecNumber evidence="2">2.7.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_04660</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="2"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GF22"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000003">
        <property type="entry name" value="NAD kinase b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000013">
        <property type="entry name" value="NAD kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF0">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="56"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFG06069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="551" mass="62041" checksum="FD1FCD55D04866C7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GF23</accession>
    <name>A0AAU9GF23_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase C-like 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05038</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GF23"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20831">
        <property type="entry name" value="C1_dGM13116p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08678">
        <property type="entry name" value="C2_C21orf25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039934">
        <property type="entry name" value="C2CD2/C2CD2L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21119">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21119:SF5">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="5">
        <location>
            <begin position="358"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="6">
        <location>
            <begin position="803"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="70"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="568"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="649"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="649"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="663"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="678"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="698"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG06570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="872" mass="94706" checksum="5E7D344BEC0617B1" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GF26</accession>
    <name>A0AAU9GF26_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="39"/>
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        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Ig_Titin_like"/>
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        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform C"/>
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        <property type="entry name" value="Bent, isoform J"/>
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        <property type="entry name" value="Myosin light chain kinase"/>
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        <property type="entry name" value="Myosin-binding protein C, slow type"/>
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        <property type="entry name" value="Protein CBR-UNC-22"/>
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        <property type="entry name" value="Titin a"/>
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        <property type="entry name" value="Titin isoform E"/>
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        <property type="entry name" value="titin isoform X1"/>
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        <property type="entry name" value="titin isoform X1"/>
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        <property type="entry name" value="twitchin isoform X2"/>
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        <property type="entry name" value="twitchin isoform X4"/>
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        <property type="entry name" value="Uncharacterized protein, isoform C"/>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
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        <property type="entry name" value="Uncharacterized protein, isoform H"/>
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        <property type="entry name" value="Uncharacterized protein, isoform I"/>
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        <property type="entry name" value="Uncharacterized protein, isoform I"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000051">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000553">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:000559">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:000748">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="78"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
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        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF151">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="39"/>
    </dbReference>
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        <property type="entry name" value="I-set"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="40"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="38"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0728">SH3 domain</keyword>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAU9GF29_DROMD</name>
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            <fullName evidence="2">calcium/calmodulin-dependent protein kinase</fullName>
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        <name evidence="15" type="ORF">DMAD_13627</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15 16">RF1</strain>
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        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
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            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
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        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
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            <dbReference type="ChEBI" id="CHEBI:61977"/>
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        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007613">
        <property type="term" value="P:memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14086">
        <property type="entry name" value="STKc_CaMKII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000009">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000001">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000002">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013543">
        <property type="entry name" value="Ca/CaM-dep_prot_kinase-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08332">
        <property type="entry name" value="CaMKII_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="14"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="320"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG06658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="530" mass="59847" checksum="B0D504484276A9D8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GF30</accession>
    <name>A0AAU9GF30_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitogen-activated protein kinase kinase kinase kinase</fullName>
            <ecNumber evidence="9">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_05039</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Serine/threonine kinase that plays a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="9"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GF30"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008349">
        <property type="term" value="F:MAP kinase kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06613">
        <property type="entry name" value="STKc_MAP4K3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000031">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021160">
        <property type="entry name" value="MAPKKKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF18">
        <property type="entry name" value="HAPPYHOUR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038172">
        <property type="entry name" value="MAPKKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="26"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="15">
        <location>
            <begin position="567"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="441"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="491"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="441"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="493"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="530"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="32"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038172-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038172-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG06585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="926" mass="102683" checksum="C5F91BC80A376F75" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GF36</accession>
    <name>A0AAU9GF36_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
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        <property type="protein sequence ID" value="BFG06708.1"/>
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        <property type="component" value="Chromosome dot"/>
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        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
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        <property type="term" value="P:sarcomere organization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="35"/>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig_sub2"/>
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        <property type="entry name" value="Ig_V-set"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    <proteinExistence type="inferred from homology"/>
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    <sequence length="1747" mass="201688" checksum="54902D9EE9545AC2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GF80</accession>
    <name>A0AAU9GF80_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">calcium/calmodulin-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_13627</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="2"/>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GF80"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007613">
        <property type="term" value="P:memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14086">
        <property type="entry name" value="STKc_CaMKII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000009">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000001">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000002">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013543">
        <property type="entry name" value="Ca/CaM-dep_prot_kinase-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08332">
        <property type="entry name" value="CaMKII_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="14"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG06660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="508" mass="57512" checksum="32A54BDFFB73A4CC" modified="2024-11-27" version="1">MAAPAACTRFSDNYDIKEELGKGAFSIVKRCVQKSTGFEFAAKIINTKKLTARDFQKLEREARICRKLHHPNIVRLHDSIQEENYHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESVNHCHQNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGDHQAWFGFAGTPGYLSPEVLKKEPYGKSVDIWACGVILYILLVGYPPFWDEDQHRLYSQIKAGAYDYPSPEWDTVTPEAKNLINQMLTVNPNKRITASEALKHPWICQRERVASVVHRQETVDCLKKFNARRKLKGAILTTMLATRNFSSRSMITKKGDGSQVKESTDSSSTTLEDDDIKEDKKGIVDRSTTVISKEPEARRQEIIKITEQLIEAINSGDFDGYTKICDPHLTAFEPEALGNLVEGIDFHKFYFENVLGKNCKAINTTILNPHVHLLGEEAACIAYVRLTQYIDKQGHAHTHQSEETRVWHKRDNKWQNVHFHRSASGKISGATTFDFIPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GF82</accession>
    <name>A0AAU9GF82_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_04921</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GF82"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017071">
        <property type="term" value="C:intracellular cyclic nucleotide activated cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005222">
        <property type="term" value="F:intracellularly cAMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005223">
        <property type="term" value="F:intracellularly cGMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000002">
        <property type="entry name" value="Cyclic nucleotide gated channel alpha 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000100">
        <property type="entry name" value="Cyclic nucleotide-gated cation channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000001">
        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050866">
        <property type="entry name" value="CNG_cation_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638:SF4">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="459"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="585"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="689"/>
            <end position="810"/>
        </location>
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                <person name="Yamamoto D."/>
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    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EPP5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14132">
        <property type="entry name" value="STKc_CK2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000059">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000088">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="37"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF88948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="304" mass="36141" checksum="F1B3FF391E4CA42D" modified="2024-11-27" version="1">MTLPSAARVYTDVNAHKPDEYWDYENYVVDWGNQDDYQLVRKLGRGKYSEVFEAINITTTEKCVVKILKPVKKKKIKREIKILENLRGGTNIITLLAVVKDPVSRTPALIFEHVNNTDFKQLYQTLTDYEIRYYLFELLKALDYCHSMGIMHRDVKPHNVMIDHENRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLRHSRKRWERFVHSDNQHLVSPEALDFLDKLLRYDHVDRLTAREAMAHPYFVPIVNGQMNPNNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EPR1</accession>
    <name>A0AAU9EPR1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Acetylcholine receptor subunit alpha-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_07828</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EPR1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098655">
        <property type="term" value="P:monoatomic cation transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007271">
        <property type="term" value="P:synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000022">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit alpha4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="18">
        <location>
            <begin position="2"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFF88963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="72" mass="8186" checksum="128EF2E57147DF45" modified="2024-11-27" version="1">MQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVLVGTAGIILQAPTLYDDRIPIIEQKDLDFSGTSAGRCRPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EQ18</accession>
    <name>A0AAU9EQ18_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Dynein heavy chain 5 axonemal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_07853</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097729">
        <property type="term" value="C:9+2 motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001221">
        <property type="entry name" value="Axonemal dynein heavy chain 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000003">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000004">
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    <sequence length="4562" mass="522413" checksum="B87706A5C0E2E847" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXF1</accession>
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8 9">RF1</strain>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF91548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="542" mass="60930" checksum="3224FF7C79ABA079" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXF2</accession>
    <name>A0AAU9EXF2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Syntaxin-1A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_09523</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EXF2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006836">
        <property type="term" value="P:neurotransmitter transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15880">
        <property type="entry name" value="SNARE_syntaxin1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.70:FF:000042">
        <property type="entry name" value="Syntaxin 11b, tandem duplicate 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000005">
        <property type="entry name" value="Syntaxin 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF424">
        <property type="entry name" value="SYNTAXIN-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00503">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="14">
        <location>
            <begin position="195"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="40"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF91180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="291" mass="33650" checksum="D75564BDAC770CE6" modified="2024-11-27" version="1">MTKDRLAALHAAQSDDEESEDVAVNVDGHDSYMDDFFSQVEEIRGMIDKVQDNVEEVKKKHSAILSAPQTDEKTKQELEDLMADIKKNANRVRGKLKGIEQNIEQEEQQNKSTADLRIRKTQHSTLSRKFVEVMTEYNRTQTDYRERCKGRIQRQLEITGRPTNDDELEKMLEEGNSSVFTQGIIMETQQAKQTLADIEARHQDIMKLETSIKELHDMFMDMAMLVESQGEMIDRIEYHVEHAMDYVQTATQDTKKALKYQSKARRKKIMILICLTVLGILAASYVSSYFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXG2</accession>
    <name>A0AAU9EXG2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">Mitochondrial Rho GTPase</fullName>
            <ecNumber evidence="15">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_09533</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion outer membrane</location>
            <topology evidence="1 15">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the mitochondrial Rho GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="15"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EXG2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01893">
        <property type="entry name" value="Miro1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01892">
        <property type="entry name" value="Miro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000011">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000255">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001271">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001641">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013566">
        <property type="entry name" value="EF_hand_assoc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013567">
        <property type="entry name" value="EF_hand_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021181">
        <property type="entry name" value="Miro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052266">
        <property type="entry name" value="Miro-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020860">
        <property type="entry name" value="MIRO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08355">
        <property type="entry name" value="EF_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08356">
        <property type="entry name" value="EF_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037488">
        <property type="entry name" value="Mt_Rho_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51423">
        <property type="entry name" value="MIRO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 15" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="619"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="18">
        <location>
            <begin position="9"/>
            <end position="192"/>
        </location>
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    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="328"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="18">
        <location>
            <begin position="441"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFF91195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="649" mass="73723" checksum="E2D6F836D8A47929" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXG5</accession>
    <name>A0AAU9EXG5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytoplasmic aconitate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Citrate hydro-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_09364</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the aconitase/IPM isomerase family.</text>
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    <dbReference type="EC" id="4.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EXG5"/>
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        <property type="component" value="Chromosome O"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072350">
        <property type="term" value="P:tricarboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01586">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01580">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000001">
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    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000005">
        <property type="entry name" value="cytoplasmic aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
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        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01341">
        <property type="entry name" value="aconitase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006757">
        <property type="entry name" value="PRK09277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009520">
        <property type="entry name" value="PRK12881.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0408">Iron</keyword>
    <keyword evidence="8 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="13">
        <location>
            <begin position="72"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="14">
        <location>
            <begin position="703"/>
            <end position="829"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF90962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="899" mass="98711" checksum="935F3AD73ADD36AD" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXG8</accession>
    <name>A0AAU9EXG8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Octopamine receptor Oamb</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_09811</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EXG8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15063">
        <property type="entry name" value="7tmA_Octopamine_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000351">
        <property type="entry name" value="Octopamine receptor Oamb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF174">
        <property type="entry name" value="TYRAMINE_OCTOPAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="557"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="37"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="237"/>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Yamamoto D."/>
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</entry>
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        <property type="entry name" value="Voltage-gated potassium channels"/>
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        <property type="entry name" value="RCK_N"/>
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    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0630">Potassium</keyword>
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        <location>
            <begin position="49"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
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    <feature type="transmembrane region" description="Helical" evidence="18">
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    <feature type="transmembrane region" description="Helical" evidence="18">
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            <end position="333"/>
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        <location>
            <begin position="356"/>
            <end position="498"/>
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        <location>
            <begin position="830"/>
            <end position="974"/>
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    </feature>
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        <location>
            <begin position="681"/>
            <end position="713"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="746"/>
            <end position="785"/>
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    </feature>
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        <location>
            <begin position="1170"/>
            <end position="1200"/>
        </location>
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        <location>
            <begin position="746"/>
            <end position="776"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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            <dbReference type="ARBA" id="ARBA00022882"/>
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            <dbReference type="ARBA" id="ARBA00022958"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF91893.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1200" mass="133236" checksum="249BA647E306BCA2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0T8</accession>
    <name>A0AAU9F0T8_DROMD</name>
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        <recommendedName>
            <fullName evidence="6">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="10" type="ORF">DMAD_09430</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10 11">RF1</strain>
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        <text evidence="1 5">Belongs to the OSBP family.</text>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
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        <property type="entry name" value="Oxysterol-binding protein-like"/>
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        </location>
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            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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    <feature type="region of interest" description="Disordered" evidence="7">
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        <location>
            <begin position="288"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="310"/>
            <end position="319"/>
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        <location>
            <begin position="391"/>
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        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF91059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="954" mass="106728" checksum="9EF31C83EBD0C5F9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0U1</accession>
    <name>A0AAU9F0U1_DROMD</name>
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        <submittedName>
            <fullName evidence="21">Glutamate-gated chloride channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_07964</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glutamate-gated chloride channel (TC 1.A.9.4) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9F0U1"/>
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        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="C:postsynaptic membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008068">
        <property type="term" value="F:extracellularly glutamate-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd18993">
        <property type="entry name" value="LGIC_ECD_GluCl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19062">
        <property type="entry name" value="LGIC_TM_GluCl"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glutamate-gated chloride channel alpha"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="glutamate-gated chloride channel isoform X1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GABAA/Glycine_rcpt"/>
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        <property type="entry name" value="GluCl_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
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        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
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        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
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        <property type="entry name" value="Neuro_actylchol_rec"/>
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    </dbReference>
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        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
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        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="34"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="251"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00061654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF89149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="454" mass="52142" checksum="6F9198BD5900215C" modified="2024-11-27" version="1">MGSGHYFWAIFYFASLCSASLANNAKINFREKEKKVLDQILGAGKYDARIRPSGINGTDGPAIVRINLFVRSIMTISDIKMEYSVQLTFREQWTDERLKFDDIQGRLKYLTLTEANRVWMPDLFFSNEKEGHFHNIIMPNVYIRIFPNGSVLYSIRISLTLACPMNLKLYPLDRQICSLRMASYGWTTNDLVFLWKEGDPVQVVKNLHLPRFTLEKFLTDYCNSKTNTGEYSCLKVDLLFKREFSYYLIQIYIPCCMLVIVSWVSFWLDQGAVPARVSLGVTTLLTMATQTSGINASLPPVSYTKAIDVWTGVCLTFVFGALLEFALVNYASRSGSNKANMHKENMKKKRRDLEQASLDAASDLLDTDSNATFAMKPLVRHPGDPLALEKRLQCEVHMQAPKRPNCCKTWLSKFPTRSKRIDVISRITFPLVFALFNLVYWSTYLFREEEDETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0U4</accession>
    <name>A0AAU9F0U4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein strawberry notch-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_09439</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the SBNO family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057332">
        <property type="entry name" value="SBNO_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026937">
        <property type="entry name" value="SBNO_Helicase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026741">
        <property type="entry name" value="SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039187">
        <property type="entry name" value="SNO_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706:SF30">
        <property type="entry name" value="PROTEIN STRAWBERRY NOTCH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706">
        <property type="entry name" value="STRAWBERRY NOTCH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13871">
        <property type="entry name" value="Helicase_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25373">
        <property type="entry name" value="SBNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Strawberry notch AAA" evidence="3">
        <location>
            <begin position="166"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Strawberry notch helicase C" evidence="2">
        <location>
            <begin position="669"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="domain" description="SBNO alpha/beta" evidence="4">
        <location>
            <begin position="986"/>
            <end position="1104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF91074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1180" mass="134490" checksum="DAA0114BE6CBD9CE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0V2</accession>
    <name>A0AAU9F0V2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_07974</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F0V2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032394">
        <property type="entry name" value="Anoct_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF83">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16178">
        <property type="entry name" value="Anoct_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="573"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="616"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="662"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="791"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="938"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin dimerisation" evidence="11">
        <location>
            <begin position="184"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="10">
        <location>
            <begin position="405"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="61"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF89164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1063" mass="121964" checksum="EED75A6F21717982" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F166</accession>
    <name>A0AAU9F166_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_08688</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the chitin synthase family. Class IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000139">
        <property type="entry name" value="Chitin synthase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055120">
        <property type="entry name" value="Chs-1/2_IV_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF42">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23000">
        <property type="entry name" value="ChitinSynthase_IV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="319"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="430"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="488"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="974"/>
            <end position="991"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
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            <end position="1021"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1027"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1060"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1298"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1355"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase chs-1/2 N-terminal putative transporter" evidence="16">
        <location>
            <begin position="92"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1182"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1585"/>
            <end position="1621"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1093"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1188"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1607"/>
            <end position="1621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF23000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF90096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1621" mass="183372" checksum="AFE1B4B2D4F6B897" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F173</accession>
    <name>A0AAU9F173_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Dual E2 ubiquitin-conjugating enzyme/E3 ubiquitin-protein ligase BIRC6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">BIR repeat-containing ubiquitin-conjugating enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Baculoviral IAP repeat-containing protein 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Ubiquitin-conjugating BIR domain enzyme apollon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="ORF">DMAD_09354</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="31 32">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Microtubule organizing center</location>
            <location evidence="4">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="2">trans-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="24">Belongs to the BIRC6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
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    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23810">
        <property type="entry name" value="UBCc_BIRC6"/>
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    <sequence length="5034" mass="554393" checksum="E6866D110C42BA34" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
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                <person name="Yamamoto D."/>
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        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR48094">
        <property type="entry name" value="PROTEIN/NUCLEIC ACID DEGLYCASE DJ-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0558">Oxidation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="5">
        <location>
            <begin position="4"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF92312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="187" mass="19311" checksum="B7EECA930E65D48D" modified="2024-11-27" version="1">MSQTALIILAPGAEEMEFVIAADVLRRAGIKVTVAGLKDSEPVKCSRDVVIVPDTSLAKAAGDKFDVVVLPGGLGGSNAMGESAAVGDLLRAQESGGGLIAAICAAPTVLAKHGIATGKSLTSYPSMKDKLVDKYCYVDDKSVVKDGNLITSRGPGTAYDFALKIAEELTGLEKVKEVAKGLLLSYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAW1</accession>
    <name>A0AAU9FAW1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Filamin-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_10767</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the filamin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21311">
        <property type="entry name" value="CH_dFLNA-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21315">
        <property type="entry name" value="CH_dFLNA-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000140">
        <property type="entry name" value="FiLamiN (Actin binding protein) homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002162">
        <property type="entry name" value="filamin-A isoform X10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001678">
        <property type="entry name" value="filamin-A isoform X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001682">
        <property type="entry name" value="filamin-A isoform X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000006">
        <property type="entry name" value="Filamin-B isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000092">
        <property type="entry name" value="Filamin-B isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000008">
        <property type="entry name" value="Filamin-B isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000007">
        <property type="entry name" value="Filamin-B isoform C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000001">
        <property type="entry name" value="Filamin-C isoform b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000096">
        <property type="entry name" value="filamin-C isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044801">
        <property type="entry name" value="Filamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537:SF8">
        <property type="entry name" value="FILAMIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537">
        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="20"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="174"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="298"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="408"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="508"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="607"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="703"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="797"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="894"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="990"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1089"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1182"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1281"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1377"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1481"/>
            <end position="1582"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1582"/>
            <end position="1674"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1672"/>
            <end position="1762"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1765"/>
            <end position="1857"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1855"/>
            <end position="1955"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1958"/>
            <end position="2050"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="2052"/>
            <end position="2145"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="2147"/>
            <end position="2238"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="2275"/>
            <end position="2369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="67"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF92787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2369" mass="256414" checksum="C8B95921FEE0AD2E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAW9</accession>
    <name>A0AAU9FAW9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_10815</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FAW9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030837">
        <property type="term" value="P:negative regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14513">
        <property type="entry name" value="DSP_slingshot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000004">
        <property type="entry name" value="Protein phosphatase Slingshot homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043587">
        <property type="entry name" value="Phosphatase_SSH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043588">
        <property type="entry name" value="SSH-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45864:SF2">
        <property type="entry name" value="PROTEIN PHOSPHATASE SLINGSHOT"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
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</entry>
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                <person name="Yamamoto D."/>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="63"/>
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            <end position="111"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="286"/>
            <end position="307"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <end position="344"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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        <location>
            <begin position="45"/>
            <end position="475"/>
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            <dbReference type="ARBA" id="ARBA00004651"/>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF92932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="491" mass="53114" checksum="785D343B38D65DE5" modified="2024-11-27" version="1">MPSVGHVEDTVGQYSYTEVSSFQVSEGNTRNMSPEPVKTGRIFMAAVAANLSAFVVGTCLGWTSPIGPKLKTEDTSDSPLDRPITSDEDAWISSLIAIGALIAPFVAGPLADRIGRKWVLLSSSLFFVLAFGINMGASEVWILYLSRLIQGFGVGFVMTVQPMYVGEISTDNVRGATGSLMQLFIVAGILYVYAIGPFVSYMALQWCCIVVPIIFDVVFYFMPESPHYFAGKGRKTDALRSLQFLRGQSAEGVHDEMAEIQSSVEEAMASKGTIMDLFKNPGNRKALFICAGLISFQQLSGINVVLFNSQSIFASANTGLDPAIATIIIGCVQVASSGLTPIVADRLGRKVMLLTSASVMTVGLTALGAFFYMQLVVGDISSVVWMPVPALVIYNIVYCTGFGPLPWAVLGEMFPANIKSSASSIVASTCWTLGFLVTYFYPALDALGSYYAFWLFAGFMVVAFFFVLFVVMETKGLSLQEIQDRLNSKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FB89</accession>
    <name>A0AAU9FB89_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein osiris 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_10912</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012464">
        <property type="entry name" value="DUF1676"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21879">
        <property type="entry name" value="FI03362P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="OSIRIS 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07898">
        <property type="entry name" value="DUF1676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043605673" description="Protein osiris 10" evidence="3">
        <location>
            <begin position="19"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="272"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF92973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="38441" checksum="9FCB2CEE10377AC9" modified="2024-11-27" version="1">MLLLYTGLLLLLLGAIAGNDFSHVGSAELKQFKQCVRGASGQRPRLSECLGRSALNFIQRVEESDNVSFVEDFASVKSDTAASRSLANVLDTDPVDFRGILENAGAVMGQRSLEWHMDGLYPGLMFKIGPTTDANSVAEFVLDGAGVQGERQFGYEDPSAGRLLAKQYLLPFLLGLKFNLVALVPLLFAGICLLLKKSLFLVKLAVYVSSFLGLGGVVSSLGGLGGGLSGGLGGFGGFGGGSSFGFQGHRPVGHFPGKTTVFGHGDELHHQQQQQYDVHEASPYRRSERKVRFEQPRAAAETTEKPQAPTEDRFYDFEQQRRASNKLLEQEDTLMRSNFQAGMQGWQAVDVRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBR2</accession>
    <name>A0AAU9FBR2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Triosephosphate isomerase</fullName>
            <ecNumber evidence="6 11">5.3.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_11040</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="6 11"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBR2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000025">
        <property type="entry name" value="Triosephosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139:SF2">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="9 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 11 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF93139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="303" mass="33076" checksum="75A0634C6E21DEF2" modified="2024-11-27" version="1">MDNNQSVCDTMLDVPKSVAPQAQKFTAVFIKLFEHQWKTYEGQKKFYANSNTHSKMSARKFCVGGNWKMNGDQKSIAEISKTLSGAALDPNTEVVIGCPAIYLMYARNLLPCTVGVAGQNAYKVAKGAFTGEISPAMLKDIGADWVILGHSERRAIFNESDELIAEKAEHALAEGLKVIACIGETLDEREAGKTNEVVARQMCAYAKKVRDWTNVVVAYEPVWAIGTGKTATPEQAQEVHAFLRQWLTDNISKEVSAALRIQYGGSVTAANAKDLAKKPDIDGFLVGGASLKPEFVDIINARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBR9</accession>
    <name>A0AAU9FBR9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ferritin</fullName>
            <ecNumber evidence="15">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_11015</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Oligomer of 12 light (L) chains and 12 heavy (H) chains; L and H chains are disulfide-linked. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble ferric iron core is deposited.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the ferritin family.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="15"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBR9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01056">
        <property type="entry name" value="Euk_Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.10:FF:000017">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF43">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="4 15" id="KW-0409">Iron storage</keyword>
    <keyword evidence="6 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043314112" description="Ferritin" evidence="16">
        <location>
            <begin position="21"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Ferritin-like diiron" evidence="17">
        <location>
            <begin position="36"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601519-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFF93110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="206" mass="23361" checksum="D7CEC6DADFD3D24B" modified="2024-11-27" version="1">MVKLIVSLLVLAVVAQQAYGDFKCSLEVPEIPKGWMDLKDGCLNGMRHQIQEEINASYQYLAMGAYFARDSVNRPGFAETFFKSAKEEREHGSKLIEYLSMRGQLTDNVSNLINVPIVAKQEWKDGAAALEDALELETKVTRSIRKLIQTCEGKPYNHYHLVDYLTGVYLEEQLHGQRELAGKLTTLKKMMSTHGELGEFLFDKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBS5</accession>
    <name>A0AAU9FBS5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Prolyl 4-hydroxylase subunit alpha-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_10996</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBS5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043695240" evidence="3">
        <location>
            <begin position="28"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Prolyl 4-hydroxylase N-terminal" evidence="4">
        <location>
            <begin position="29"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="391"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="37"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="272"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="397"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF93074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="490" mass="54589" checksum="858C270B0A394FE5" modified="2024-11-27" version="1">MQMPGAVVGLLWLLQVSLLWSTVGCHASSEEQLEALLATETLLIDELREYIERLDQQLEEIKRETAAIEEIHSQVGDGVEEYIGNPLNVLILLKRFETMWPRLEQLANATHNISARADRDRDMDLILPTEDDYEEALSNLLHLQSVYELEPASLSLGVVNGMKLGSAMSWSDCLEMAVKSDAGVAKFWLETALDKLPPATNGTDHRSERERGKVQILEATLNMEYRAGALSEALATADELLLLRPMNQNVQKAKAKIERALAKTSTQLPSSRGHKPKPKTKKSKSAEQLLIEELCRGATQEATTGGRSTHCKLERSTPWSILQPVRVELLCSDPYIALHHDVLTLKQSDQLLGLMDVEEETGRGASHQPLKFSMLAQKKLRTIRHQMGHVSGEMDPWEGRRHGHEHTTRPEESAQGQLHQTRVIMLNLQAPGMGGAVVFPQLELGVNVPRGALLHWRTRTSSTSEMDYRSRQAVCPVLLGVQVSAWTGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBS6</accession>
    <name>A0AAU9FBS6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Calcium-binding mitochondrial carrier protein Aralar1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_11005</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="12">Homodimer (via N-terminus).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBS6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015183">
        <property type="term" value="F:L-aspartate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043490">
        <property type="term" value="P:malate-aspartate shuttle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000416">
        <property type="entry name" value="Aralar1, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000396">
        <property type="entry name" value="Calcium-binding mitochondrial carrier protein Aralar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000004">
        <property type="entry name" value="Calcium-binding mitochondrial carrier protein Aralar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051028">
        <property type="entry name" value="Mito_Solute_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678:SF9">
        <property type="entry name" value="CALCIUM-BINDING MITOCHONDRIAL CARRIER PROTEIN ARALAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="163"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="340"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="439"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="531"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF93096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="691" mass="76449" checksum="BC8306E8D714D428" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBT7</accession>
    <name>A0AAU9FBT7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Autophagy-related protein 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_11042</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat ATG16 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF93142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBT7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034274">
        <property type="term" value="C:Atg12-Atg5-Atg16 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22887">
        <property type="entry name" value="Atg16_CCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000648">
        <property type="entry name" value="Autophagy-related 16, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000515">
        <property type="entry name" value="Autophagy-related 16, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045160">
        <property type="entry name" value="ATG16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013923">
        <property type="entry name" value="Autophagy-rel_prot_16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19878">
        <property type="entry name" value="AUTOPHAGY PROTEIN 16-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19878:SF8">
        <property type="entry name" value="AUTOPHAGY-RELATED 16, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08614">
        <property type="entry name" value="ATG16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Autophagy-related protein 16" evidence="7">
        <location>
            <begin position="12"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="315"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="359"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="401"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="459"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="571"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="129"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF93142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="605" mass="67439" checksum="5D8E7F978B607CE9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FC33</accession>
    <name>A0AAU9FC33_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_11123</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Unusual broad substrate spectrum amino acid:sodium cotransporter that promotes absorption of the D isomers of essential amino acids. Neutral amino acids are the preferred substrates, especially methionine and phenylalanine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FC33"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10324">
        <property type="entry name" value="SLC6sbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF321">
        <property type="entry name" value="SODIUM-DEPENDENT NUTRIENT AMINO ACID TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 15" id="KW-0769">Symport</keyword>
    <keyword evidence="4 15 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="307"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="408"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="445"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="474"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="516"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="557"/>
            <end position="578"/>
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    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="30"/>
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            <begin position="1"/>
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        <location>
            <position position="72"/>
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        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
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        <location>
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        <ligand>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29101"/>
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        <location>
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        <ligand>
            <name>Na(+)</name>
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        <location>
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        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
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    <sequence length="636" mass="70549" checksum="17D826A580DDD158" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FC39</accession>
    <name>A0AAU9FC39_DROMD</name>
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        <recommendedName>
            <fullName evidence="4">Protein yellow</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="ORF">DMAD_11139</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
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        <subcellularLocation>
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        </subcellularLocation>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJG0</accession>
    <name>A0AAU9FJG0_DROMD</name>
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        <recommendedName>
            <fullName evidence="6">protein-glutamine gamma-glutamyltransferase</fullName>
            <ecNumber evidence="6">2.3.2.13</ecNumber>
        </recommendedName>
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        <name evidence="11" type="ORF">DMAD_13199</name>
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    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutaminyl-[protein] + L-lysyl-[protein] = [protein]-L-lysyl-N(6)-5-L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:54816"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:14005"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:138370"/>
            <dbReference type="EC" id="2.3.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="9">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglutaminase superfamily. Transglutaminase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.13" evidence="6"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJG0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003810">
        <property type="term" value="F:protein-glutamine gamma-glutamyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000090">
        <property type="entry name" value="Protein-glutamine gamma-glutamyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.260.10:FF:000001">
        <property type="entry name" value="Protein-glutamine gamma-glutamyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000171">
        <property type="entry name" value="protein-glutamine gamma-glutamyltransferase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002167">
        <property type="entry name" value="Transglutaminase, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050779">
        <property type="entry name" value="Transglutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023608">
        <property type="entry name" value="Transglutaminase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008958">
        <property type="entry name" value="Transglutaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036238">
        <property type="entry name" value="Transglutaminase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001102">
        <property type="entry name" value="Transglutaminase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590">
        <property type="entry name" value="PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590:SF69">
        <property type="entry name" value="RE08173P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00927">
        <property type="entry name" value="Transglut_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00868">
        <property type="entry name" value="Transglut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000459">
        <property type="entry name" value="TGM_EBP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49309">
        <property type="entry name" value="Transglutaminase, two C-terminal domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 9" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="10">
        <location>
            <begin position="315"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="499"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="504"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000459-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000459-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF95893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="769" mass="87110" checksum="32FF3FC38D291DA3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJG1</accession>
    <name>A0AAU9FJG1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_13120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJG1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF95790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="138" mass="14700" checksum="28A43D60751D6DF0" modified="2024-11-27" version="1">MEEADHLLSYHSSSSAADVEVDMDVDVDNDAVSLLMPELDSATALDCGNFVDYDEFNLCLNNILSEEDDDSGGAGNGNGGGNGSGIASKHQHQQWRDASDVLNIVQLNNDLGLSFQEFVSETTADEQRSVSSSSPQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJG8</accession>
    <name>A0AAU9FJG8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">GTPase-activating protein skywalker</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_13078</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJG8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000035">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="7">
        <location>
            <begin position="84"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="8">
        <location>
            <begin position="408"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF95736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="587" mass="67282" checksum="850BB8BB1B654231" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJG9</accession>
    <name>A0AAU9FJG9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Homeotic protein spalt-major</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_13140</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for the establishment of the posterior-most head and the anterior-most tail segments of the embryo. Probably function as a transcriptional regulator. Could repress the transcription of the tsh gene.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the sal C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048699">
        <property type="term" value="P:generation of neurons"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002381">
        <property type="entry name" value="Putative spalt protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000708">
        <property type="entry name" value="Sal-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000025">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000215">
        <property type="entry name" value="Spalt-like transcription factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000130">
        <property type="entry name" value="Spalt-like transcription factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000291">
        <property type="entry name" value="Spalt-like transcription factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051565">
        <property type="entry name" value="Sal_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233:SF87">
        <property type="entry name" value="HOMEOTIC PROTEIN SPALT-MAJOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233">
        <property type="entry name" value="SAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="461"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="489"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
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    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="720"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="17"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="874"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1162"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1197"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1288"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="20"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="874"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1202"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1292"/>
            <end position="1308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF96208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1526" mass="169557" checksum="6FB740F433A13F58" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKD0</accession>
    <name>A0AAU9FKD0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-adenosylmethionine synthase</fullName>
            <ecNumber evidence="11">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13374</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="11">Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AdoMet synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="11"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKD0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18079">
        <property type="entry name" value="S-AdoMet_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000001">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000003">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000004">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00086">
        <property type="entry name" value="S_AdoMet_synth1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="S-adenosylmethionine synthetase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="13">
        <location>
            <begin position="28"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase central" evidence="14">
        <location>
            <begin position="139"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="15">
        <location>
            <begin position="262"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF96115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="406" mass="44696" checksum="1657972C34E7FF15" modified="2024-11-27" version="1">MPQKTNGHSANGCNGNGNSYDMEDGQTFLFTSESVGEGHPDKMCDQISDAILDAHLKQDPNAKVACETVAKTGMILLCGEITSKAVVDYQKVVRETVQHIGYDDSSKGFDFKTCNVLLALDQQSPEIAAGVHVNRPEEEIGAGDQGIMFGYATDETEECMPLTVVLAHKLNEKIAELRRSDVFWWARPDSKTQVTCEYLFNQGSAVPKRVHTIVVSMQHSEKISLETLRSEVMEKVVKVVIPAKYFDANTIVHINPCGLFVIGGPMGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFTKVDRSAAYAARWVAKSLVKAGLCKRCLVQVSYAIGLAEPLSITVFDYGTSHKTQKELLEIIKRNFDLRPGKIVKDLNLRQPIYQRTSTYGHFGRDGFSWEEAKHLEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKD3</accession>
    <name>A0AAU9FKD3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein FAM92A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_13593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKD3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009602">
        <property type="entry name" value="CBAR/FAM92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21223">
        <property type="entry name" value="CBY1-INTERACTING BAR DOMAIN-CONTAINING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21223:SF2">
        <property type="entry name" value="CBY1-INTERACTING BAR DOMAIN-CONTAINING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06730">
        <property type="entry name" value="FAM92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF96386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="387" mass="43900" checksum="A143173CEC18DA20" modified="2024-11-27" version="1">MFRRGKLSFLNTKDDRIKVINERITLTERHLMEICSAFALVTRKMAKYRDSYDELAKGVKGYADDEEINESLSTGLKSFTNAVTIMGDYMDINVHRLEHKIVNEMSQFEQLCKSTRDNLRLAVIARDKEVLRQRQMLEIKSKFAANNSAADSELFKAKLEVARTNKEIDEIIGNFEQRKLRDIKQLLGDFILISMKQHTKALEVLSASYYDIGSIDERDDYLEFQKLMKTKEEPTTLKGTLKKGLRSQSMDSLDHEQLVSPLKRHQKLSRSSKNLAGAGIAHGNKGEEQQEQETDEQEDEEVDEDDDDEEETEQSGEDDEGEETGSASDDEREPAAPDAPPAPRENVFGVKPRASSSKEMSNPSMAPLKPIRQSVKHPFKAVAATHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKD6</accession>
    <name>A0AAU9FKD6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable G-protein coupled receptor0</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_13360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKD6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF96093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="190" mass="20827" checksum="34EE2F5AE34921BF" modified="2024-11-27" version="1">MTMDTTTTTTGDDGQPGPEEAELLRQFRRLFAPILDDSLQLYYKLNEPDRLDTEHIRIHQTVAQLNELTSSEEETIVTQVNSPSPSPSPSPPVDVELLLPLSSCSSTSSSSLYAIHTPSPAHPSGLLLQPQYLDSPTLLQSCSESRTITEQVQLHNPPQQLNEDENNTSCSLSSNLTDSKTLDGRTPIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKD9</accession>
    <name>A0AAU9FKD9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_13289</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKD9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000008">
        <property type="entry name" value="Apoptosis-resistant E3 ubiquitin protein ligase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000013">
        <property type="entry name" value="Apoptosis-resistant E3 ubiquitin protein ligase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000975">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058738">
        <property type="entry name" value="PH-like_AREL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF340">
        <property type="entry name" value="APOPTOSIS-RESISTANT E3 UBIQUITIN PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25916">
        <property type="entry name" value="AREL1_PH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="Filamin" evidence="6">
        <location>
            <begin position="261"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="9">
        <location>
            <begin position="593"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="18"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="895"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF96005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="928" mass="104596" checksum="8B475A398326C4E0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRW9</accession>
    <name>A0AAU9FRW9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Mediator of RNA polymerase II transcription subunit 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Mediator complex subunit 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_06606</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Mediator complex subunit 14 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056877">
        <property type="entry name" value="Med14_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055122">
        <property type="entry name" value="Med14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055113">
        <property type="entry name" value="Med14_RM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055114">
        <property type="entry name" value="Med14_RM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055107">
        <property type="entry name" value="Med14_RM8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013947">
        <property type="entry name" value="Mediator_Med14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056879">
        <property type="entry name" value="RM3_Med14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056878">
        <property type="entry name" value="RM5_Med14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12809">
        <property type="entry name" value="MEDIATOR COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12809:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08638">
        <property type="entry name" value="Med14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25069">
        <property type="entry name" value="Med14_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22981">
        <property type="entry name" value="RM2_Med14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25065">
        <property type="entry name" value="RM3_Med14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25067">
        <property type="entry name" value="RM5_Med14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22984">
        <property type="entry name" value="RM6_Med14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22983">
        <property type="entry name" value="RM8_Med14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0010">Activator</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit MED14 N-terminal" evidence="12">
        <location>
            <begin position="31"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator of RNA polymerase II transcription subunit 14 RM2" evidence="13">
        <location>
            <begin position="300"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator of RNA polymerase II transcription subunit 14 RM3" evidence="16">
        <location>
            <begin position="376"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator of RNA polymerase II transcription subunit 14 RM5" evidence="17">
        <location>
            <begin position="651"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator of RNA polymerase II transcription subunit 14 RM5" evidence="17">
        <location>
            <begin position="713"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator of RNA polymerase II transcription subunit 14 RM6" evidence="15">
        <location>
            <begin position="816"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator of RNA polymerase II transcription subunit 14 RM8" evidence="14">
        <location>
            <begin position="1234"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator of RNA polymerase II transcription subunit 14 C-terminal" evidence="18">
        <location>
            <begin position="1327"/>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Release of an N-terminal amino acid, preferentially alanine, from a wide range of peptides, amides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="18">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.14" evidence="14"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRY4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016285">
        <property type="term" value="F:alanyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF174">
        <property type="entry name" value="PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 24" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 18" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="23">
        <location>
            <begin position="151"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="21">
        <location>
            <begin position="370"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="22">
        <location>
            <begin position="668"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="105"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="443"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="19">
        <location>
            <position position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00081993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="BFF98452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1001" mass="114182" checksum="91FA60634E4723C2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRY8</accession>
    <name>A0AAU9FRY8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_06842</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRY8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001955">
        <property type="entry name" value="Jim, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000683">
        <property type="entry name" value="Zinc finger protein 252"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000505">
        <property type="entry name" value="zinc finger protein 32 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="369"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="397"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="425"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="537"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="565"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="593"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="621"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="649"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="69"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Diptera</taxon>
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                <person name="Yamamoto D."/>
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        <location>
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        <location>
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    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="623"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF98953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="701" mass="79400" checksum="97E67BD06ABDB224" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FT47</accession>
    <name>A0AAU9FT47_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Probable chitinase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_07198</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FT47"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02872">
        <property type="entry name" value="GH18_chitolectin_chitotriosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000013">
        <property type="entry name" value="Probable chitinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000097">
        <property type="entry name" value="Probable chitinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
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        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF403">
        <property type="entry name" value="CHITINASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043459850" evidence="6">
        <location>
            <begin position="33"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="7">
        <location>
            <begin position="40"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF99251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="483" mass="53861" checksum="183DE81A0C1943E7" modified="2024-11-27" version="1">MRQREVWGEAPQLWLLLLLASVSCTLWASVDARTGPSHDKVVVCYISTWAVYRPEQGAYAIDNFDPNLCTHVVYAFAGLDITQASIKSLDPWQDLKEQYGKGGYEQLTGLKRSHPHLKVSLAIGGWNEGSANYSSLVGNSYLRERFVKQVSSFVRKYNFDGLDLDWEYPTQRGGKPSDRENFVALTKELRDEFDNHGLLLTSAIGAAKKVIDEAYDVRQISRYLDYLHIMCYDYHGSWDQRVGYNSPLAAPAEDPLSVKFTIDYLLKLGAPPSKLVLGLPFYGRTFKTVSEGNLNDASDGVGFRGPYTREDGFLGYNEICNILSNKTSGWTKQWDPQTSQVLARSERNVFTQDVSVVTFDSSRSIANKVLYAMSKRLAGVMVWSADTDDFLGNCELDSDTFEDFKVVSSAPRRLSQSYPLLRTINEATVLALEQLAVPEPVPDDSENEIPHGSIADRKNAAASLVSLSVTATALFMLLHSLAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FT52</accession>
    <name>A0AAU9FT52_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Basic helix-loop-helix neural transcription factor TAP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_06752</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FT52"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF98656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="158" mass="18116" checksum="1E0AFF438ACAF9B1" modified="2024-11-27" version="1">MFGCGRMFPYGQTQQQQQHPEGHGHQHPHPHHPHHQQPQMLPFQPGMEYSAAAPHPHPHPHPQHATPTGFDFGSSMRYYQQEPRPQQQAPPAGQFSQEKFDLFRGSFEAAANLPSTATEPAMHQQSSFYTQTPPWKEYPEEPHPQPHPHSYKQFAPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FT54</accession>
    <name>A0AAU9FT54_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mediator of RNA polymerase II transcription subunit 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_06651</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FT54"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF98495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="428" mass="49588" checksum="8BAC3932F7DB64C4" modified="2024-11-27" version="1">MVLRSGIIIPFQFRDTKKLLMIGVPEENRNLNIWDLKNVVRAAFGIYNFEFRNKKIGFNIPDELLLHYLAQRHDLTNFVIEISQALDDGHAKEMLSYEPSCSMAVLKQQQQQQQQQHHPLQHHVPLPQRSSESASHHTHDYVPTSQPNSPALGVCHEPVDSPLEQQANTSSSDHADSSQKLRLTQTYAERTPESLTQSIDPMDIIPKAETESEVERIARASRFLARQEQQHHQHQQQQQQHQQQQQAAALQHALPAQQIFPNYTHSLPPMNAPNPTQQSPYTPGLMSRFRKRGERMSKDQKELYVKFFEDNPCMLSNHRRHDGLTEPLWAKLAHMLNSVPQGAVKNVEDWKQTFDAWRYRIFMYTRYNSKLSMSETSDPKNFKPLTATDQKAYAMWTSHKHIAPQDYEKMDLFVPLDEATTATNSYDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FT59</accession>
    <name>A0AAU9FT59_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein argonaute-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_06751</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FT59"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3Y0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043717589" evidence="3">
        <location>
            <begin position="23"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG02255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="445" mass="48356" checksum="644A981D4C0A11AD" modified="2024-11-27" version="1">MTFYWWSHWFWITCLSVQLLSSHVHTYPRSQQEATQPVVRQTTKLYQTTVSQTWSTATQSNIMDLTNKPTTAAAAAVTPVYAEEDEDESENEDQEEEEQHEDLLLNDGEAAQEEEQQEAGTDAVEVEQTVAQYEFKRSADFTSEQLNNFTNFSSSTSTNASSNSSSSNSSSSTLKPALNSSSSSSSPATTTTTTAASTSSTLDTSSAATTAATTATEASTTGVVMAVGVAAAAAAATVTVTTPLTSSATPSTPKINTEWLSDELLEHGEKAPFTLENANKEEELRRAESEHRSRKSKVLSAEYKHINRYINFSSDTKSLLTRSASAAPSVADGYDGANDEGNISSEALAIQRTYFLDAGAISAICFTVFGICCTVGTIGIVLYRRRFLNKPQALSEPDSSVYIDDSTMRDNSDEMYSLDNDSFLNSLEAMTIQNYWTDTVKHTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3Y1</accession>
    <name>A0AAU9G3Y1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin-conjugating enzyme E2 S</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">E2 ubiquitin-conjugating enzyme S</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquitin carrier protein S</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Ubiquitin-protein ligase S</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_02257</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3Y1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010458">
        <property type="term" value="P:exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23804">
        <property type="entry name" value="UBCc_UBE2S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000034">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="17">
        <location>
            <begin position="13"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="164"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="14">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFG02864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="208" mass="23331" checksum="E49FF6223C19D501" modified="2024-11-27" version="1">MSSYSNVENLSPQTIRQVMRELQEMETTPPEGIKVLINESDVTDIQALIDGPAGTPYAVGIFRVKLTLSKDFPQTPPKAYFLTKIFHPNVASNGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHAQPAKCGVSAASDAKDDDGPSTKKHAGLDKKLQDKKKEKLLKEKKRMLKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3Y5</accession>
    <name>A0AAU9G3Y5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA-binding protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_02218</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3Y5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12373">
        <property type="entry name" value="RRM_SRSF3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000899">
        <property type="entry name" value="RNA-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050907">
        <property type="entry name" value="SRSF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
        <property type="entry name" value="SERINE/ARGININE RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="11"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="76"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="91"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="133"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFG02809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="162" mass="17197" checksum="661DAF66F645697E" modified="2024-11-27" version="1">MPRYREWDLACKVYVGNLGSSASKYEIENAFSKYGPLRNVWVARNPPGFAFVEFEDRRDAEDATRGLDGTRCCGTRIRVEMSSGRSREGRGGGGGGGGGGGGGGGGGPRRGARSGSGGGGGGGARTGDGGGRYRSRSPRRSRTRSRSFSRDRRSRSDSRDRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3Y6</accession>
    <name>A0AAU9G3Y6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Plexin domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_02077</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="BFG02610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
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        <property type="entry name" value="PLXDC"/>
    </dbReference>
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        <property type="entry name" value="LD40707P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13055">
        <property type="entry name" value="TUMOR ENDOTHELIAL MARKER 7 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01608">
        <property type="entry name" value="BACINVASINC"/>
    </dbReference>
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    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
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    <feature type="chain" id="PRO_5043639228" evidence="9">
        <location>
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            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="596"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="518"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
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            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="563"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG02610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="655" mass="70253" checksum="564D7DE2882AD6FB" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3Y7</accession>
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        <recommendedName>
            <fullName evidence="9">Phosphodiesterase</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="12" type="ORF">DMAD_02067</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Vital for female fertility. Required for learning/memory.</text>
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    <comment type="cofactor">
        <cofactor evidence="9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="9">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
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    <dbReference type="EMBL" id="AP029266">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="project" value="GOC"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:circadian rhythm"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:conditioned taste aversion"/>
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        <property type="term" value="P:nonassociative learning"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18100">
        <property type="entry name" value="PDE4_UCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0114">cAMP</keyword>
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    <keyword evidence="2 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="11">
        <location>
            <begin position="246"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
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            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="599"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="599"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="632"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="655"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="322"/>
            <end position="326"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="531"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053071"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG02598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="664" mass="72998" checksum="5CCF6A4BAE889447" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G490</accession>
    <name>A0AAU9G490_DROMD</name>
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        <submittedName>
            <fullName evidence="16">Protein sidekick</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_01876</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the sidekick family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome A"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G492</accession>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="18 19">RF1</strain>
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</entry>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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                <person name="Yamamoto D."/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 13"/>
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        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 13"/>
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        <location>
            <begin position="1624"/>
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        </location>
    </feature>
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        <location>
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        <location>
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        <location>
            <begin position="2400"/>
            <end position="2412"/>
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    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG03467.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2412" mass="272946" checksum="F0BFBCEACE6D42A0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G543</accession>
    <name>A0AAU9G543_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">MAP/microtubule affinity-regulating kinase 3</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Serine/threonine-protein kinase par-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">DMAD_02833</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Regulates localization and activity of MITF by mediating its phosphorylation, promoting subsequent interaction between MITF and 14-3-3 and retention in the cytosol. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1. Phosphorylates PKP2 and KSR1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with MAPT/TAU. Interacts with DLG5 (via coiled-coil domain). Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5. Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ. Interacts with PKP2 (via N-terminus). Interacts with CDC25C. Interacts with KSR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
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    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G543"/>
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        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
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    </dbReference>
    <dbReference type="CDD" id="cd12196">
        <property type="entry name" value="MARK1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="STKc_MARK"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KP78b, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000001">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049508">
        <property type="entry name" value="MARK1-4_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
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    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 27" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="485"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="25">
        <location>
            <begin position="755"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="26">
        <location>
            <begin position="1111"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="71"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="287"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="797"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="921"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="996"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="37"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="313"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="397"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="805"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="842"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="923"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="956"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
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        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="514"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00054424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00063680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00074935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="BFG03623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1160" mass="126819" checksum="38811DB6D6B46D25" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G545</accession>
    <name>A0AAU9G545_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_02209</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G545"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20811">
        <property type="entry name" value="C1_Raf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01816">
        <property type="entry name" value="RBD_RAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14062">
        <property type="entry name" value="STKc_Raf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000024">
        <property type="entry name" value="B-Raf proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000036">
        <property type="entry name" value="RAF proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000259">
        <property type="entry name" value="Raf serine/threonine-protein kinase phl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003116">
        <property type="entry name" value="RBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF262">
        <property type="entry name" value="RAF HOMOLOG SERINE_THREONINE-PROTEIN KINASE RAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02196">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00455">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50898">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RBD" evidence="15">
        <location>
            <begin position="155"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="14">
        <location>
            <begin position="236"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="412"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="107"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="353"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="363"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="384"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG02797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="722" mass="81680" checksum="E467776EC67514B9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G549</accession>
    <name>A0AAU9G549_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BAH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_02120</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005677">
        <property type="term" value="C:chromatin silencing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04714">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053032">
        <property type="entry name" value="BAH_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46576">
        <property type="entry name" value="BROMO ADJACENT HOMOLOGY DOMAIN-CONTAINING 1 PROTEIN"/>
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    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025956">
        <property type="entry name" value="DYNC1I1/DYNC1I2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF22">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 INTERMEDIATE CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11540">
        <property type="entry name" value="Dynein_IC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="511"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="17"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG02649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="651" mass="72716" checksum="6AA1279B9D2417D6" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G554</accession>
    <name>A0AAU9G554_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cyclic AMP-dependent transcription factor ATF-6 alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_02429</name>
        <name evidence="3" type="ORF">DMAD_02430</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G554"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG03088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG03090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="48" mass="5380" checksum="6230622FCE95F0EA" modified="2024-11-27" version="1">MVGPKNQAAAAVSHEKPRLVQFNNSIANNSRQMSNARRRESLQAKFEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G556</accession>
    <name>A0AAU9G556_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">MAP/microtubule affinity-regulating kinase 3</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Serine/threonine-protein kinase par-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">DMAD_02833</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Regulates localization and activity of MITF by mediating its phosphorylation, promoting subsequent interaction between MITF and 14-3-3 and retention in the cytosol. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1. Phosphorylates PKP2 and KSR1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with MAPT/TAU. Interacts with DLG5 (via coiled-coil domain). Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5. Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ. Interacts with PKP2 (via N-terminus). Interacts with CDC25C. Interacts with KSR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G556"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12196">
        <property type="entry name" value="MARK1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14072">
        <property type="entry name" value="STKc_MARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14337">
        <property type="entry name" value="UBA_MARK_Par1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001032">
        <property type="entry name" value="KP78b, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000001">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049508">
        <property type="entry name" value="MARK1-4_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02149">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 27" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="485"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="25">
        <location>
            <begin position="755"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="26">
        <location>
            <begin position="1123"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="71"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="287"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="361"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="800"/>
            <end position="864"/>
        </location>
    </feature>
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        </source>
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</entry>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
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                <person name="Yamamoto D."/>
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</entry>
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        </lineage>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <location>
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        </location>
    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="97" mass="10857" checksum="6A852B68758EB4C6" modified="2024-11-27" version="1">MVLPEELLDNEEYEDIRTDIEQECSKFGEVLGLKIPRPHGKGADTGTPPKGCGKVYVHFATIEDSEKALGALSGRKFSGRIVIGSFFNRDKYFEDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDY3</accession>
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        <submittedName>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. RGK family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDY3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005246">
        <property type="term" value="F:calcium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000664">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051641">
        <property type="entry name" value="RGK_GTP-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45775">
        <property type="entry name" value="RAD, GEM/KIR FAMILY MEMBER 2, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45775:SF7">
        <property type="entry name" value="RAD, GEM_KIR FAMILY MEMBER 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="49"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="123"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="181"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="233"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="470"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG06228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="526" mass="58871" checksum="7381B0FD95A2E76C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDY4</accession>
    <name>A0AAU9GDY4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein mahjong</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_04493</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VPRBP/DCAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033270">
        <property type="entry name" value="VPRBP/DCAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13129:SF4">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13129">
        <property type="entry name" value="VPRBP PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1441"/>
            <end position="1538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1444"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1484"/>
            <end position="1538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG05851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1538" mass="171635" checksum="6DDC4AEAEF6B1B97" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDZ4</accession>
    <name>A0AAU9GDZ4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Host cell factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_13668</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035097">
        <property type="term" value="C:histone methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000008">
        <property type="entry name" value="host cell factor 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000015">
        <property type="entry name" value="host cell factor 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043536">
        <property type="entry name" value="HCF1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059124">
        <property type="entry name" value="Kelch_HCF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46003">
        <property type="entry name" value="HOST CELL FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46003:SF1">
        <property type="entry name" value="HOST CELL FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13854">
        <property type="entry name" value="Kelch_HCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="1350"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="815"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1113"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1251"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1561"/>
            <end position="1608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="815"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1114"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1136"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1187"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1261"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1562"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1582"/>
            <end position="1596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG06767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1608" mass="168907" checksum="956160BE7761B541" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDZ5</accession>
    <name>A0AAU9GDZ5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glutamate transporter polyphemus-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_04393</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDZ5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF340">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="6">
        <location>
            <begin position="2"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG05720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="413" mass="46563" checksum="4E3657B727E493CF" modified="2024-11-27" version="1">MAFAYTGWMCGAILLTLTTIMLIHGITLLIMCMVESARRQQQGYCNFSDTMVFAFNEGPKWCKYLAKATGYLVDGVLSLSHYGICVVYLVFVAVNMKQLLEHYVQAMDLRIYIAIVGLCSIPFFLVRHLKYLVPFNMVANIAMYLGFFLIFYYLFQDLPPLSDRPMFNEPSKYPLFFGIVLFSVSSVGVMLAVESEMSHPEQYIGWFGVLNLSAVVVVISYLFFSIMAYWRYGPHVHGSVTLDLPTDEVISQLSKAFISLALFLSFPLSGYVTIDIVFNHYMNHGNRLRNPHFVEYIVRVFFVVLSTVNAVAFPNLGPLVAFVGALTISLLNLVFPACIDLCLNYHEPYTYGKLRWKLVKNILLIIIGVVILGYCCTLSVLDMIEEYGGSGRKTSSLEKLPLGVLTDIDMTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDZ6</accession>
    <name>A0AAU9GDZ6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DMAD_04485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDZ6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13333">
        <property type="entry name" value="M-AAA PROTEASE-INTERACTING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13333:SF5">
        <property type="entry name" value="M-AAA PROTEASE-INTERACTING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFG05842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="206" mass="23895" checksum="5140895CDFE2A9D0" modified="2024-11-27" version="1">MVSQLRSQLKETRIYLQERYFMWKLRYLLGPDFGKEEFLEGTCQATAAMLEAVRRVDWRSIRNFCTEEFAPAMYSIARRESVYANLMRLQADDVEVSLPVTFKRSKGHNGQSFVYVDLGIVGMRFVRVKPDELMEISGMPLTVPSHQQILLATYKITFRKELSGPDGSCPKDEAWLFNAFKLRDMSLMGFCPQSGECWFIRAVKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEU0</accession>
    <name>A0AAU9GEU0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14114">
        <property type="entry name" value="STKc_Twitchin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000321">
        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000440">
        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000097">
        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000642">
        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000614">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001845">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001892">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000460">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000504">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000505">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000147">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002083">
        <property type="entry name" value="Protein CBR-UNC-22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000160">
        <property type="entry name" value="Titin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000003">
        <property type="entry name" value="Titin isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000012">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000127">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001003">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000249">
        <property type="entry name" value="twitchin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000056">
        <property type="entry name" value="twitchin isoform X4"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000936">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000006">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000775">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000805">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
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            <begin position="298"/>
            <end position="319"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="587"/>
            <end position="596"/>
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            <end position="1099"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
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    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG06764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEU9</accession>
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        <submittedName>
            <fullName evidence="13">Protein enabled</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_05037</name>
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    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
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        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Lamellipodium</location>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="PROTEIN ENABLED"/>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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            <end position="451"/>
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            <begin position="747"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00009785"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50196"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50229"/>
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        <source>
            <dbReference type="EMBL" id="BFG06553.1"/>
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    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="844" mass="90618" checksum="AF4BD17FBF85A5A9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="2">Moesin/ezrin/radixin homolog 1</fullName>
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        <name evidence="8" type="ORF">DMAD_05041</name>
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        <name type="scientific">Drosophila madeirensis</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <strain evidence="8 9">RF1</strain>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Endopterygota</taxon>
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            <taxon>Ephydroidea</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="BFG06645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="919" mass="105513" checksum="26CF08EDE8FA3843" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GF86</accession>
    <name>A0AAU9GF86_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14114">
        <property type="entry name" value="STKc_Twitchin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform C"/>
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    </dbReference>
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        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="5"/>
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        <property type="entry name" value="Bent, isoform J"/>
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        <property type="entry name" value="Bent, isoform J"/>
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        <property type="entry name" value="Bent, isoform J"/>
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    </dbReference>
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        <property type="entry name" value="Myosin light chain kinase"/>
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        <property type="entry name" value="Myosin-binding protein C, slow type"/>
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        <property type="entry name" value="Protein CBR-UNC-22"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="Titin a"/>
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        <property type="entry name" value="titin isoform X1"/>
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        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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        <property type="match status" value="71"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
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    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
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        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
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        <property type="match status" value="26"/>
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0728">SH3 domain</keyword>
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        <location>
            <begin position="9"/>
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        <location>
            <begin position="1899"/>
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        <location>
            <begin position="2001"/>
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        <location>
            <begin position="2098"/>
            <end position="2194"/>
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    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="2200"/>
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        <location>
            <begin position="2299"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF88753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="719" mass="78827" checksum="82362C7C93014DE5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EP90</accession>
    <name>A0AAU9EP90_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">SH2B adapter protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_07713</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SH2B adapter family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EP90"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005068">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01231">
        <property type="entry name" value="PH_SH2B_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10346">
        <property type="entry name" value="SH2_SH2B_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000354">
        <property type="entry name" value="Lnk, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000008">
        <property type="entry name" value="SH2B adapter protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015012">
        <property type="entry name" value="Phe_ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036290">
        <property type="entry name" value="Phe_ZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030523">
        <property type="entry name" value="SH2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035057">
        <property type="entry name" value="SH2B1_SH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10872:SF2">
        <property type="entry name" value="LNK, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10872">
        <property type="entry name" value="SH2B ADAPTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08916">
        <property type="entry name" value="Phe_ZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109805">
        <property type="entry name" value="Phenylalanine zipper"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="192"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="6">
        <location>
            <begin position="447"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="552"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="566"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="588"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="598"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF88796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="626" mass="66354" checksum="2BFD0380EBD0F180" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXE5</accession>
    <name>A0AAU9EXE5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Dual E2 ubiquitin-conjugating enzyme/E3 ubiquitin-protein ligase BIRC6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">BIR repeat-containing ubiquitin-conjugating enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Baculoviral IAP repeat-containing protein 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Ubiquitin-conjugating BIR domain enzyme apollon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="ORF">DMAD_09354</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="31 32">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Microtubule organizing center</location>
            <location evidence="4">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="24">Belongs to the BIRC6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23810">
        <property type="entry name" value="UBCc_BIRC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000014">
        <property type="entry name" value="Baculoviral IAP repeat-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000001">
        <property type="entry name" value="baculoviral IAP repeat-containing protein 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022103">
        <property type="entry name" value="BIRC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116">
        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116:SF39">
        <property type="entry name" value="BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12356">
        <property type="entry name" value="BIRC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="23" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="22" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-0967">Endosome</keyword>
    <keyword evidence="20" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="21" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="19" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="17" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0853">WD repeat</keyword>
    <keyword evidence="18" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="UBC core" evidence="30">
        <location>
            <begin position="4607"/>
            <end position="4774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="498"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="650"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="979"/>
            <end position="1005"/>
        </location>
    </feature>
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        <location>
            <begin position="2308"/>
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        </location>
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        <location>
            <begin position="3652"/>
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    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="3908"/>
            <end position="3933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="4266"/>
            <end position="4326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="4920"/>
            <end position="4959"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="501"/>
            <end position="533"/>
        </location>
    </feature>
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        <location>
            <begin position="651"/>
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        </location>
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        <location>
            <begin position="979"/>
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        <location>
            <begin position="991"/>
            <end position="1005"/>
        </location>
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        <location>
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            <begin position="4275"/>
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            <begin position="4937"/>
            <end position="4959"/>
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            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004198"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00069601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00075349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00079718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00081222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="EMBL" id="BFF90947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="4959" mass="546032" checksum="B5097BAEB2443E6D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXH0</accession>
    <name>A0AAU9EXH0_DROMD</name>
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        <submittedName>
            <fullName evidence="13">Phosphatidylinositol-binding clathrin assembly protein LAP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_08306</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Binds clathrin and phosphatidylinositol 4,5-bisphosphate.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
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        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
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        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PICALM/SNAP91 family.</text>
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        <property type="protein sequence ID" value="BFF89574.1"/>
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        <property type="component" value="Chromosome O"/>
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        <property type="term" value="C:clathrin-coated pit"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:extrinsic component of presynaptic endocytic zone membrane"/>
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        <property type="term" value="C:synaptic vesicle"/>
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        <property type="term" value="F:1-phosphatidylinositol binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
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        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
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        <property type="term" value="F:SNARE binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:clathrin-dependent endocytosis"/>
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        <property type="term" value="P:locomotion"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
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        <property type="entry name" value="ANTH_N_AP180"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol-binding clathrin assembly protein LAP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="phosphatidylinositol-binding clathrin assembly protein-like isoform X1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ANTH_dom"/>
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        <property type="entry name" value="ANTH_dom_sf"/>
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        <property type="entry name" value="AP180-like"/>
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        <property type="entry name" value="ENTH"/>
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        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0168">Coated pit</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="12">
        <location>
            <begin position="16"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="568"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="667"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="747"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="568"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="769"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF89574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="789" mass="84625" checksum="5A102D508A84724B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXH1</accession>
    <name>A0AAU9EXH1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Probable cytochrome P450 313a4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_08959</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EXH1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11057">
        <property type="entry name" value="CYP313-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF189">
        <property type="entry name" value="CYTOCHROME P450 4C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="421"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF90435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="475" mass="54740" checksum="275B91A034DBF5BC" modified="2024-11-27" version="1">MLTWTLWCGLLFVLWIYFLWSRRRFYLLALKIPGPVGYPILGMAHKLMRREDILNEFGIYLAKHGPTIFSWLGPIPFMIVSDPQVVQDILTSPHCVNKGIIYKAVDDGAGIGLFSLKDPRWSVHRKLLNPAFGHKVLLSFLPIFNKETAQLLDQLEALPGGGEHDLIPLLQSLTLGIATQTTMGCDVKSEENFKSNSLMERYQCRWARQLAGKEDNYHRAKTEIRAFIRKLIQKRLAEDTEDSIKSKDKNIFLNLATDLLRRGVFTLTNVEDESNVIVFGAFETTANTVYYILMLLAMFPQYQERAFEEIKSLFPDGGDFEVTYEDTQQMKYMDLVINESMRVIPPVPVVSRQTAQDLTLTNGIVIPKGVQIAINMFHMHRSKQIWGPEAEIFNPDNFLPHNSRDKHPYAFIPFTKGIRNCIGWRYALLSIKVTLAKLLRNYSFKTSFKFEDLYYVEDITMKLKAVPKLEIEKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXI3</accession>
    <name>A0AAU9EXI3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Phosphatidylinositol-binding clathrin assembly protein LAP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_08306</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Binds clathrin and phosphatidylinositol 4,5-bisphosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PICALM/SNAP91 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EXI3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098894">
        <property type="term" value="C:extrinsic component of presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16985">
        <property type="entry name" value="ANTH_N_AP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000017">
        <property type="entry name" value="Phosphatidylinositol-binding clathrin assembly protein LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000001">
        <property type="entry name" value="phosphatidylinositol-binding clathrin assembly protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0168">Coated pit</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="12">
        <location>
            <begin position="16"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="598"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="648"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="307"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="670"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF89589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="690" mass="73201" checksum="75625E5EC2A6ADE7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXJ1</accession>
    <name>A0AAU9EXJ1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">UDP-D-xylose:beta-D-glucoside alpha-1,3-D-xylosyltransferase</fullName>
            <ecNumber evidence="11">2.4.2.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_09930</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Glycosyltransferase which elongates the O-linked glucose attached to EGF-like repeats in the extracellular domain of Notch proteins by catalyzing the addition of xylose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3-O-(beta-D-glucosyl)-L-seryl-[EGF-like domain protein] + UDP-alpha-D-xylose = 3-O-[alpha-D-xylosyl-(1-&gt;3)-beta-D-glucosyl]-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56064"/>
            <dbReference type="Rhea" id="RHEA-COMP:14610"/>
            <dbReference type="Rhea" id="RHEA-COMP:14611"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:140575"/>
            <dbReference type="ChEBI" id="CHEBI:140576"/>
            <dbReference type="EC" id="2.4.2.42"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.42" evidence="11"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EXJ1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </source>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="716" mass="79878" checksum="D900D781C8113E11" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0S0</accession>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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            <strain evidence="9 10">RF1</strain>
        </source>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Endopterygota</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF90689.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0X6</accession>
    <name>A0AAU9F0X6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial glutamate carrier 2</fullName>
        </recommendedName>
        <alternativeName>
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        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Solute carrier family 25 member 18</fullName>
        </alternativeName>
    </protein>
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        <name evidence="20" type="ORF">DMAD_08568</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
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        <text evidence="13">Responsible for the transport of glutamate from the cytosol into the mitochondrial matrix with the concomitant import of a proton (symport system).</text>
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        <reaction evidence="12">
            <text>L-glutamate(in) + H(+)(in) = L-glutamate(out) + H(+)(out)</text>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
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    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
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    <dbReference type="GO" id="GO:0005743">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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</entry>
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            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_08577</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F0Y5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14126">
        <property type="entry name" value="STKc_CK1_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000703">
        <property type="entry name" value="Casein kinase I gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022247">
        <property type="entry name" value="Casein_kinase-1_gamma_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12605">
        <property type="entry name" value="CK1gamma_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="62"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="385"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="400"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF89946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="441" mass="50068" checksum="CE7A1DCD33CCDBDD" modified="2024-11-27" version="1">MQRRERQASGGVPQTTTAGPSAGNVANATTALAGGKSSSNNMYSTRQSVSTTTGVLMVGPNFRVGKKIGCGNFGELRLGKNLYNNEHVAIKMEPMKSKAPQLHLEYRFYKLLGSHAETAPDGIPRIYHLGTCGGRYNAMVLELLGLSLEDLFNICARKFSLKTVLMIAKQLLHRIEYVHSRHLIYRDVKPENFLIGRTSTKREKIIHIIDFGLAKEYIDLDTNRHIPYREHKSLTGTARYMSINTHMGREQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRATPIEVLCDGHPEEFATYLRYVRRLDFFETPDYDFLRRLFQDLFERKGYTDEGEFDWTGKTMSTPVGSLQTGHEVIISPNKDRHNVTAKVVSSTNGELNPDDPTAGHSNTPITQQPEVEVVDETNGSLYSRCCCFFKRKKKKSTRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0Y9</accession>
    <name>A0AAU9F0Y9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Very low-density lipoprotein receptor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_08023</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F0Y9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050685">
        <property type="entry name" value="LDLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF89224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="207" mass="23917" checksum="2145D8215B0E1E74" modified="2024-11-27" version="1">MRIFTDPSVCDNTSDWFHCWESNVGEGTCTGEPNSTWIRNTQVCNGLADCPNGEDESILECKHEPKLSNNSYFYCATGVAIEMKQKCDGEVDCSDGSDEMQTKCQPGKQINRRGNCKGHWSFEYRPGECVREIGLCDGKYDCSNGFDESLEVCYQQWHNETHTRLQISSWHQWHKIIFAFRSAEGAVHTHEKIYCQELRVEYLRCEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F142</accession>
    <name>A0AAU9F142_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_08651</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F142"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032589">
        <property type="term" value="C:neuron projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001026">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037448">
        <property type="entry name" value="Zig-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279:SF21">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 11, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE DPR -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044020885" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="193"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="334"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF90051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="482" mass="52873" checksum="9989766D8D1F885F" modified="2024-11-27" version="1">MPVSFLTYFAALAASAAPMGENASLIAPTTFSPTITMAAAVVGVTGVEGAGAVMRLRGDGNQAAAMEATTVEQLQQDQQQQQQTVDIWSERPDTATLTKGFSIPTYLPPFPDFIPADLPQIQRNASAWARNPTDGRLMPSNEHPPQLQSPTQGQGQSQSQSQSQPRLHAYDSEQKELQLRREKELAKEREQLPRRQLSLPPINASVQAGQHAYLPCKLNQHSNKPLSWVRLRDEHIIAVDHTTFINDARFASLLQSTTSTAPDPPSTTSTATTPTAIMVIPGNASSPEERGNGSSLSWTLQIKYVRLEDAGWYECQLATEPKMSAKVHLFVITPRTELIGDRQRFVKAGSKVELHCIVRGTLEAPKYIFWYRGEQQVTAENEASGGQSGWYTQIDRNIFGSTEHNRNTIGSLVIPLVRKIHSGNYTCEPENSAAVSMQLHVLSGEYSASAIKSAAAGLHRLGHGYSSLHQWLIFLLVALNKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F193</accession>
    <name>A0AAU9F193_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_09589</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02659">
        <property type="entry name" value="peptidase_C19C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056850">
        <property type="entry name" value="ARM_UBP34_24_USP9X_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021905">
        <property type="entry name" value="DUF3517"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055176">
        <property type="entry name" value="UBP24/USP9X/USP9Y_UBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF925">
        <property type="entry name" value="UBIQUITINYL HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25010">
        <property type="entry name" value="ARM_UBP24_USP9X-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12030">
        <property type="entry name" value="DUF3517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22900">
        <property type="entry name" value="UCH_UBL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="1704"/>
            <end position="2097"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="952"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1106"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1200"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2606"/>
            <end position="2674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2709"/>
            <end position="2744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2767"/>
            <end position="2851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="9"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1109"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1204"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="2614"/>
            <end position="2623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="2632"/>
            <end position="2642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="2643"/>
            <end position="2652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="2660"/>
            <end position="2673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="2715"/>
            <end position="2728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="2729"/>
            <end position="2744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="2767"/>
            <end position="2777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="2817"/>
            <end position="2843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF91269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2851" mass="317911" checksum="379D70E5F03407A8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9D6</accession>
    <name>A0AAU9F9D6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_10388</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F9D6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="13"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="129"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF92304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="432" mass="49909" checksum="B4E00D2493E89434" modified="2024-11-27" version="1">MMARLSGCVGTHWTGPCRAKHSRNQKKASSTRKQRSGCAKRSNNSAKDRNANRNKNPHRLETQEEQQPGEQPSSGVTPCCNPPKETYPLLAHHSYTIRNTYPGPLETYDGEARGEKRLICFADLSESYLNLLFNQVEEEERKEKEKEELEKQQKSGESKCQATAKRCRENSRKRSPQRNVRFCGEQKKSCGEKKKEGCAEKKKESCEEKKENCAENTKKSCGEVKKEGCAENKEKCVEKKKESCEEKKKEGCAEKQKEGCGEVKKENCGDNKKEGCAETKNAEESTKKEENCEEQKTETRNFRAMIKQETNAQRLYEKGLRRKGCQNGKAWQCLLPREKLTYYWRVRTGEQLRPTAYSNFKQGFVKRFRKMHPRAGSKRVRAETRTQWYALERSQREPFVMQALVYHVSTGELDPLDQCSVREMYTRLKGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9E0</accession>
    <name>A0AAU9F9E0_DROMD</name>
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        <submittedName>
            <fullName evidence="5">Serine-rich adhesin for platelets</fullName>
        </submittedName>
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        <name evidence="5" type="ORF">DMAD_10396</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5 6">RF1</strain>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CIN85 AND CD2AP RELATED, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
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    </dbReference>
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        <property type="entry name" value="P67PHOX"/>
    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
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        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
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            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="244"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="337"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="589"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="682"/>
            <end position="841"/>
        </location>
    </feature>
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        <location>
            <begin position="185"/>
            <end position="197"/>
        </location>
    </feature>
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        <location>
            <begin position="340"/>
            <end position="368"/>
        </location>
    </feature>
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        <location>
            <begin position="381"/>
            <end position="390"/>
        </location>
    </feature>
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        <location>
            <begin position="430"/>
            <end position="439"/>
        </location>
    </feature>
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        <location>
            <begin position="465"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="611"/>
            <end position="621"/>
        </location>
    </feature>
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        <location>
            <begin position="623"/>
            <end position="644"/>
        </location>
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        <location>
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            <end position="724"/>
        </location>
    </feature>
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        <location>
            <begin position="731"/>
            <end position="759"/>
        </location>
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            <end position="813"/>
        </location>
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        <location>
            <begin position="814"/>
            <end position="829"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF92313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="900" mass="95532" checksum="297FC364C9707E56" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9E1</accession>
    <name>A0AAU9F9E1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphoribosyl pyrophosphate synthase-associated protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_10371</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F9E1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000031">
        <property type="entry name" value="Probable PRS4-ribose-phosphate pyrophosphokinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF53">
        <property type="entry name" value="GH23275P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="4">
        <location>
            <begin position="27"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="236"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF92280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="375" mass="41604" checksum="B10E29E703512536" modified="2024-11-27" version="1">MSKRRLGSVRPPKRCRSDMDNTSTSDIVIINGNSHPDLANMVAERMGLKNGGCSVFHKSNRETIVEISDSVRGKDIYIIQTGTKDANNNIMELLIMAYACKTSSARSIVGVIPYLPYSKQCKMRKRGCIVSKLLAKMMCTSGLTHIITMDLHQKEIQGFFDIPVDNLRASPFLLQYIQESIPEYRNSVIVARNPGVAKKANSYAERLRLGLAVIHGEQKEAESDEVDGRYSPPPTSSSRQRTTSVSVGVPEHPVKVKPPLTIVGDVNGRIAIMVDDLIDDVQSFVAAAEMLKENGACKIYVLATHGLLSSDAPRLLDESCIDEIVVTNTIPHEIQKLQCHKIKTIDISILIAEAIRRIHNKESMSYLFRNVTLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9E3</accession>
    <name>A0AAU9F9E3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine protease 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_10365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F9E3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF134">
        <property type="entry name" value="AT07769P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <begin position="20"/>
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        <source>
            <dbReference type="Pfam" id="PF24985"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF92299.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
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</entry>
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            <authorList>
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                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5 6">RF1</strain>
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                <person name="Yamamoto D."/>
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</entry>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF92380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="800" mass="88682" checksum="9DC493D170AB432F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9K9</accession>
    <name>A0AAU9F9K9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="12">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_10416</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PPP phosphatase family. PP-2B subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="12"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F9K9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033192">
        <property type="term" value="F:calmodulin-dependent protein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097720">
        <property type="term" value="P:calcineurin-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030431">
        <property type="term" value="P:sleep"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07416">
        <property type="entry name" value="MPP_PP2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041751">
        <property type="entry name" value="MPP_PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043360">
        <property type="entry name" value="PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45673">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2B CATALYTIC SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="14">
        <location>
            <begin position="192"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="524"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="529"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF92337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="608" mass="67523" checksum="3D54F8754629987D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAU2</accession>
    <name>A0AAU9FAU2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Filamin-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_10767</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the filamin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21311">
        <property type="entry name" value="CH_dFLNA-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21315">
        <property type="entry name" value="CH_dFLNA-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000140">
        <property type="entry name" value="FiLamiN (Actin binding protein) homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002162">
        <property type="entry name" value="filamin-A isoform X10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001678">
        <property type="entry name" value="filamin-A isoform X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001682">
        <property type="entry name" value="filamin-A isoform X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000006">
        <property type="entry name" value="Filamin-B isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000092">
        <property type="entry name" value="Filamin-B isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000008">
        <property type="entry name" value="Filamin-B isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000007">
        <property type="entry name" value="Filamin-B isoform C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000001">
        <property type="entry name" value="Filamin-C isoform b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000096">
        <property type="entry name" value="filamin-C isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044801">
        <property type="entry name" value="Filamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537:SF8">
        <property type="entry name" value="FILAMIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537">
        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="20"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="167"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="291"/>
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    <feature type="repeat" description="Filamin" evidence="7">
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    <feature type="repeat" description="Filamin" evidence="7">
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    <feature type="repeat" description="Filamin" evidence="7">
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    <feature type="repeat" description="Filamin" evidence="7">
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    <feature type="repeat" description="Filamin" evidence="7">
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15 16">RF1</strain>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAU4</accession>
    <name>A0AAU9FAU4_DROMD</name>
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        <submittedName>
            <fullName evidence="8">Ninjurin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_10785</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8 9">RF1</strain>
        </source>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF04923">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF92809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="147" mass="16529" checksum="5DD306F214D2CAB3" modified="2024-11-27" version="1">MAQIVAGDERVNEKPVKNAWQMKAMDANRYATKKTIAQGMLDIALLTANASQLKYILQVGEQHQFYKLMLILISLSIVMQILSGVLSLSLSLLRDCRLHQPEFHQSANVINHVRTAFAFFTTMLNLFISAFDSRLPPPQGDLLNNVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAU5</accession>
    <name>A0AAU9FAU5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-actin monooxygenase</fullName>
            <ecNumber evidence="4">1.14.13.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_10737</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-methionyl-[F-actin] + NADPH + O2 + H(+) = L-methionyl-(R)-S-oxide-[F-actin] + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:51308"/>
            <dbReference type="Rhea" id="RHEA-COMP:12953"/>
            <dbReference type="Rhea" id="RHEA-COMP:12956"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.225"/>
        </reaction>
    </comment>
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        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Mical family.</text>
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    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome O"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:actin binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:F-actin monooxygenase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="entry name" value="LIM_Mical"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.10.110.10:FF:000118">
        <property type="entry name" value="protein-methionine sulfoxide oxidase Mical isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000004">
        <property type="entry name" value="protein-methionine sulfoxide oxidase MICAL2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="bMERB_dom"/>
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        <property type="entry name" value="CH_dom"/>
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        <property type="entry name" value="CH_dom_sf"/>
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        <property type="entry name" value="F-actin_Monoox_Mical"/>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
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        <property type="entry name" value="Rossman_Mical"/>
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    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF54">
        <property type="entry name" value="[F-ACTIN]-MONOOXYGENASE MICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF12130">
        <property type="entry name" value="bMERB_dom"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF25413">
        <property type="entry name" value="Rossman_Mical"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DUF3585"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BMERB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0009">Actin-binding</keyword>
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    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 16" id="KW-0440">LIM domain</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="558"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="20">
        <location>
            <begin position="1077"/>
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        <location>
            <begin position="2877"/>
            <end position="3012"/>
        </location>
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            <begin position="689"/>
            <end position="737"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="18">
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            <begin position="771"/>
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    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="958"/>
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    <feature type="region of interest" description="Disordered" evidence="18">
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            <begin position="1214"/>
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    <feature type="region of interest" description="Disordered" evidence="18">
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            <begin position="1273"/>
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    <feature type="region of interest" description="Disordered" evidence="18">
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            <begin position="1647"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1934"/>
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    <feature type="region of interest" description="Disordered" evidence="18">
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            <begin position="2160"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
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            <begin position="2221"/>
            <end position="2268"/>
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    <feature type="region of interest" description="Disordered" evidence="18">
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            <begin position="2310"/>
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    <feature type="region of interest" description="Disordered" evidence="18">
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
    <sequence length="3050" mass="335843" checksum="B848EEC0683F5390" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAU8</accession>
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        <submittedName>
            <fullName evidence="18">Synaptic functional regulator FMR1</fullName>
        </submittedName>
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        <name evidence="18" type="ORF">DMAD_10753</name>
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    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="18 19">RF1</strain>
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    <comment type="similarity">
        <text evidence="4">Belongs to the FMR1 family.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="GOC"/>
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        <property type="term" value="C:ribonucleoprotein complex"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:mRNA 3'-UTR binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:translation regulator activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:animal organ development"/>
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        <property type="term" value="P:mRNA transport"/>
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        <property type="project" value="TreeGrafter"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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                <person name="Yamamoto D."/>
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            <authorList>
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                <person name="Yamamoto D."/>
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            <strain evidence="6 7">RF1</strain>
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    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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                <person name="Yamamoto D."/>
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            <begin position="1997"/>
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            <begin position="111"/>
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            <begin position="249"/>
            <end position="266"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50003"/>
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            <dbReference type="EMBL" id="BFF92883.1"/>
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            <dbReference type="Proteomes" id="UP001500889"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FB51</accession>
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        <submittedName>
            <fullName evidence="8">Low molecular weight phosphotyrosine protein phosphatase 1</fullName>
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        <name evidence="8" type="ORF">DMAD_10837</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8 9">RF1</strain>
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        <subcellularLocation>
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        <text evidence="2">Belongs to the low molecular weight phosphotyrosine protein phosphatase family.</text>
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            <begin position="1"/>
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            <dbReference type="EMBL" id="BFF92877.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="11">Facilitated trehalose transporter Tret1-2 homolog</fullName>
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        <name evidence="11" type="ORF">DMAD_10875</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11 12">RF1</strain>
        </source>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
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    <dbReference type="EMBL" id="AP029263">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
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        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
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        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF86">
        <property type="entry name" value="FACILITATED TREHALOSE TRANSPORTER TRET1-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUGRTRNSPORT"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="115"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="142"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="225"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="373"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="405"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="476"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="44"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF92929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="512" mass="55131" checksum="E5F7E86E6C669F30" modified="2024-11-27" version="1"> </sequence>
</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
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        <subcellularLocation>
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    </dbReference>
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        <location>
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        </location>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="417" mass="45547" checksum="E339A351450BFF6A" modified="2024-11-27" version="1">MSVCESKAVVQQQLQQQLQQQAAAAAAVAAAAVAQQQQQQQQQQQQQQQQQPPQVVVPHLTPQQQQQSTQSIADYLAQLLKDRKQLAAFPNVFTHVERLLDEEIARVRASLFQINGVKKEPLTLPEPEGAVVTMNEKVYVPVREHPDFNFVGRILGPRGMTAKQLEQETGCKIMVRGKGSMRDKKKEDANRGKPNWEHLSDDLHVLITVEDTENRAKVKLAQAVGEVQKLLVPQAEGEDELKKRQLMELAIINGTYRDTTAKSVAAFSCVGSPSASVLYPAVCEEEWRRLMAGSDNRLLTPAGLPAGLAAQIRAPSSAPLGAPLILNPRMSVPTSAASILSQAPSGAFDQSGHQMIFAPYADYANYAALAGNPLLAEYADHSGAIKQQRRLAANREHPYQRATVGVPAKPAGFIEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FB56</accession>
    <name>A0AAU9FB56_DROMD</name>
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        <submittedName>
            <fullName evidence="7">Protein held out wings</fullName>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
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        </subcellularLocation>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="KH_dom"/>
    </dbReference>
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        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF125">
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    </dbReference>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF92902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="401" mass="44009" checksum="CD23488C3AEE0E70" modified="2024-11-27" version="1">MSVCESKAVVQQQLQQQLQQQAAAAAAVAAAAVAQQQQQQQQQQQQQQQQQPPQVVVPHLTPQQQQQSTQSIADYLAQLLKDRKQLAAFPNVFTHVERLLDEEIARVRASLFQINGVKKEPLTLPEPEGAVVTMNEKVYVPVREHPDFNFVGRILGPRGMTAKQLEQETGCKIMVRGKGSMRDKKKEDANRGKPNWEHLSDDLHVLITVEDTENRAKVKLAQAVGEVQKLLVPQAEGEDELKKRQLMELAIINGTYRDTTAKSVAVCEEEWRRLMAGSDNRLLTPAGLPAGLAAQIRAPSSAPLGAPLILNPRMSVPTSAASILSQAPSGAFDQSGHQMIFAPYADYANYAALAGNPLLAEYADHSGAIKQQRRLAANREHPYQRATVGVPAKPAGFIEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FB62</accession>
    <name>A0AAU9FB62_DROMD</name>
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        <submittedName>
            <fullName evidence="5">Leucine-rich repeat-containing protein 40</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_10833</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Flyers-cup, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich repeat-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000193">
        <property type="entry name" value="Leucine-rich repeat-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
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    </dbReference>
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        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
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    </dbReference>
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        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF46">
        <property type="entry name" value="LEUCINE RICH REPEAT-CONTAINING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="9"/>
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="match status" value="15"/>
    </dbReference>
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        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="4">
        <location>
            <begin position="259"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
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            <begin position="355"/>
            <end position="401"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
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        <location>
            <begin position="382"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF92871.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="702" mass="79254" checksum="A6BFE15AED2CC12A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FB65</accession>
    <name>A0AAU9FB65_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Papilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_10846</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the papilin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd22639">
        <property type="entry name" value="Kunitz_papilin_lacunin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22637">
        <property type="entry name" value="Kunitz_papilin_mig6-like"/>
        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
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        <property type="entry name" value="Collagen, type VI, alpha 3"/>
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        <property type="entry name" value="Myosin, light chain kinase a"/>
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    </dbReference>
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        <property type="entry name" value="Pancreatic trypsin inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="papilin isoform X2"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulins"/>
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        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
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        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
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        <property type="entry name" value="ADAMTS_spacer1"/>
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        <property type="entry name" value="Ig_sub2"/>
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        <property type="entry name" value="Kunitz_BPTI"/>
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        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAPILIN"/>
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        <property type="entry name" value="Kunitz_BPTI"/>
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        <property type="entry name" value="ADAMTSFAMILY"/>
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    <keyword evidence="8" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="10" id="KW-0654">Proteoglycan</keyword>
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    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0722">Serine protease inhibitor</keyword>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BFF92892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2771" mass="297487" checksum="38A9DCFB92810361" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FB69</accession>
    <name>A0AAU9FB69_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Dynein-1, subspecies f</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_10848</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Force generating protein of eukaryotic cilia and flagella. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required for assembly of the I1 inner arm complex and its targeting to the appropriate axoneme location. Also required for phototaxis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">The I1 inner arm complex (also known as the f dynein complex) is a two-headed isoform composed of two heavy chains (1-alpha and 1-beta), three intermediate chains and three light chains. I1 occupies a specific position proximal to the first radial spoke and repeats every 96 nm along the length of the axoneme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097729">
        <property type="term" value="C:9+2 motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036156">
        <property type="term" value="C:inner dynein arm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036159">
        <property type="term" value="P:inner dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000153">
        <property type="entry name" value="Dynein axonemal heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000005">
        <property type="entry name" value="Dynein axonemal heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000005">
        <property type="entry name" value="Dynein axonemal heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000006">
        <property type="entry name" value="Dynein axonemal heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000884">
        <property type="entry name" value="Dynein axonemal heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002141">
        <property type="entry name" value="Dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000008">
        <property type="entry name" value="Dynein heavy chain 10, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000001">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000002">
        <property type="entry name" value="Dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000001">
        <property type="entry name" value="dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000063">
        <property type="entry name" value="dynein heavy chain 6, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000049">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF63">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="19">
        <location>
            <begin position="2389"/>
            <end position="2525"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="19">
        <location>
            <begin position="2668"/>
            <end position="2853"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="19">
        <location>
            <begin position="3024"/>
            <end position="3177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="120"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="326"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="388"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="824"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="3906"/>
            <end position="3954"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="4202"/>
            <end position="4265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="127"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="190"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="388"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="407"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FB91</accession>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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            <strain evidence="6 7">RF1</strain>
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                <person name="Yamamoto D."/>
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    </evidence>
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    <dbReference type="PANTHER" id="PTHR10707:SF10">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02936">
        <property type="entry name" value="COX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01873">
        <property type="entry name" value="CYTCOXIDASE4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81406">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
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    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="114"/>
            <end position="135"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008135"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF92947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="180" mass="21323" checksum="A601ED5053023234" modified="2024-11-27" version="1">MRALCWQFERKLPHQMARMAMVRRATHHDGTRLMSGDREIVGYGVNGSPIYVDCVEFPFPAIRYREVTPDLCAVREKELGDWKALSLQEKKLLYRHSFCQTYAEFQHFTPDWKLVMGVGFWSVAIGLLISVWYNFKLYDPVPETYAEERRQAQLRRIIQLEMNPITGLSSKWCYHTNKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJF5</accession>
    <name>A0AAU9FJF5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Kinesin-like protein KIN-7I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_13151</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome U"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="P:microtubule-based movement"/>
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    </dbReference>
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        <property type="term" value="P:mitotic cell cycle"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Kinesin motor domain"/>
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        <property type="entry name" value="Kinesin-like_fam"/>
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        <property type="entry name" value="Kinesin_motor_CS"/>
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        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
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        <property type="entry name" value="Kinesin_motor_dom_sf"/>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF75">
        <property type="entry name" value="CENTROMERE-ASSOCIATED PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="6"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1231"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="328"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="505"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="671"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="942"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1604"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1678"/>
            <end position="1809"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1834"/>
            <end position="1889"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1918"/>
            <end position="1991"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="2099"/>
            <end position="2133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="2216"/>
            <end position="2264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="81"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF95829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2268" mass="258849" checksum="BB0B9705BF605682" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJF7</accession>
    <name>A0AAU9FJF7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">SUN domain-containing ossification factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_13046</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Interacts with EMP65.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SLP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000099">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045120">
        <property type="entry name" value="Suco/Slp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953">
        <property type="entry name" value="MEMBRANE PROTEIN CH1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953:SF0">
        <property type="entry name" value="SUN DOMAIN-CONTAINING OSSIFICATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="865"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="14">
        <location>
            <begin position="220"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="142"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="198"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="553"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="613"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="893"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1029"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1123"/>
            <end position="1171"/>
        </location>
    </feature>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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</entry>
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</entry>
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    </dbReference>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <authorList>
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                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
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            <end position="278"/>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="278" mass="31905" checksum="B9DEB498FD6F56F1" modified="2024-11-27" version="1">MSVEMDSLVEIPRGDWIKLRDLYNKRDTDPHGYMCINNFINWVQQEPELPVRVLSLNGDWQQEGTFVIAVDMGTKHQHLYFNTLSDDLSPVTKALECLKPSNDEYLFFGFSSRFDPVVRHIGKTFLTKELNFVDTVWYFASKELVSTFTIEAPSGIRLDSLRIEDAETVNEIWPHRSHNSVKVVRRQIQYSENVGAYDETGKLVAWCLRLTIGSLGLLQVLESHKRLGLGNLMVRALSKKINDLGEPVVAPVVPQNTASRRMFEKLGFQQIDKVFWAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJL7</accession>
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        <submittedName>
            <fullName evidence="3">Mucin-5AC</fullName>
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        <name evidence="3" type="ORF">DMAD_13366</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">RF1</strain>
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        </location>
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        </source>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1931" mass="213544" checksum="392D41EC52C5332F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJL8</accession>
    <name>A0AAU9FJL8_DROMD</name>
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        <submittedName>
            <fullName evidence="16">Probable cytochrome P450 4ac1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13130</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
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            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
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        <subcellularLocation>
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            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
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            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <text evidence="5 15">Belongs to the cytochrome P450 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:monooxygenase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF105">
        <property type="entry name" value="CYTOCHROME P450 4P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EP450I"/>
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        <property type="entry name" value="P450"/>
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    <dbReference type="SUPFAM" id="SSF48264">
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    </dbReference>
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        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
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    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>heme</name>
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        </ligand>
        <ligandPart>
            <name>Fe</name>
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        </ligandPart>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF95802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="507" mass="58711" checksum="F234B62F00C0F1D6" modified="2024-11-27" version="1">MLIALLGTPLLVLAVWLLLQRLNRTYFILSLTRRVRTEDGSPLESKVAVMPGTSIFGNNLDILNPTPSSLFNFVRDSAAKAQGRNFLWYFLFAPMYNVIRAEEAEEIFQSTKLITKNVIYELVRPFLGDGLLISTDQKWHARRKALTPAFHFNVLQSFLATFKEECNKLIKVLDKKLAGELDLNQVIPQFTLNNICETALGVKLDDMSEGNQYRRAIHAIEEVLIQRVCNPLMYYNWYFYLVGDYRKQVQNLRIVHDFSSRIIERKRQQFKRKQLGVVDDFGKKQRYAMLDTLLAAEADGQIDHQGICDEVNTFMFEGYDTTSTCLIFTLLMLALHEDVQEKCLEEVSGLPEDTDSISVFEFNELMYLECAIKESLRMFPSVPFIGRQCMEECVVNGMIMPKDTQISIHIYDIMRDPRHFPNQNEYQPERFLPENTVDRHPFAFVPFSAGQRNCIGQKFAILEIKVLLAAVLRNFRILPVTRLEDLIFENGIVMRTQQNVKVKLVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJM3</accession>
    <name>A0AAU9FJM3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MABP1/WDR62 second WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_13277</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000377">
        <property type="entry name" value="uncharacterized protein LOC108094137 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003560">
        <property type="entry name" value="WD repeat domain 62, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000046">
        <property type="entry name" value="WD repeat-containing protein 62 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056162">
        <property type="entry name" value="WD40_MABP1-WDR62_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052779">
        <property type="entry name" value="WDR62"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45589">
        <property type="entry name" value="WD REPEAT DOMAIN 62, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45589:SF1">
        <property type="entry name" value="WD REPEAT DOMAIN 62, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24782">
        <property type="entry name" value="WD40_MABP1-WDR62_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="MABP1/WDR62 second WD40" evidence="3">
        <location>
            <begin position="382"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="508"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="686"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="826"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="1016"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
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            <end position="1146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1167"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1314"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1609"/>
            <end position="1780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1875"/>
            <end position="1894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2015"/>
            <end position="2059"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2075"/>
            <end position="2243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="827"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1042"/>
            <end position="1061"/>
        </location>
    </feature>
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        <location>
            <begin position="1079"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1207"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1229"/>
            <end position="1245"/>
        </location>
    </feature>
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        <location>
            <begin position="1628"/>
            <end position="1661"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="1710"/>
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        </location>
    </feature>
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        <location>
            <begin position="1722"/>
            <end position="1736"/>
        </location>
    </feature>
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        <location>
            <begin position="1743"/>
            <end position="1756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1761"/>
            <end position="1770"/>
        </location>
    </feature>
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        <location>
            <begin position="2016"/>
            <end position="2040"/>
        </location>
    </feature>
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        <location>
            <begin position="2084"/>
            <end position="2133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
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            <end position="2152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2179"/>
            <end position="2201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2220"/>
            <end position="2234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF95987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2357" mass="259113" checksum="E7E30068360F452E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJM4</accession>
    <name>A0AAU9FJM4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Guanylate cyclase</fullName>
            <ecNumber evidence="3 15">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_13158</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="3 15"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJM4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001653">
        <property type="term" value="F:peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06370">
        <property type="entry name" value="PBP1_SAP_GC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14042">
        <property type="entry name" value="PK_GC-A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000019">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000279">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000498">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF501">
        <property type="entry name" value="GUANYLATE CYCLASE 32E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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    <accession>A0AAU9FRU6</accession>
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        <name type="scientific">Drosophila madeirensis</name>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">RF1</strain>
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    </dbReference>
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        <location>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1390" mass="133167" checksum="5438B56255FD8E24" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRU7</accession>
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        <recommendedName>
            <fullName evidence="11">Protein phosphatase 1 regulatory subunit 12B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Myosin phosphatase-targeting subunit 2</fullName>
        </alternativeName>
    </protein>
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        <name evidence="17" type="ORF">DMAD_06462</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="17 18">RF1</strain>
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    </reference>
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        <text evidence="9">Regulates myosin phosphatase activity. Augments Ca(2+) sensitivity of the contractile apparatus.</text>
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    <comment type="subunit">
        <text evidence="10">PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12B mediates binding to myosin. Isoform 3 and isoform 4 bind PPP1R12A, but not isoform 1 of PPP1R12B itself. Binds IL16.</text>
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        <subcellularLocation>
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    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043627959" evidence="7">
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            <begin position="22"/>
            <end position="312"/>
        </location>
    </feature>
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            <begin position="28"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="8">
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            <begin position="87"/>
            <end position="133"/>
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    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="8">
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        </location>
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    <feature type="domain" description="Chitin-binding type-2" evidence="8">
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            <begin position="214"/>
            <end position="270"/>
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    </feature>
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            <begin position="273"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00022669"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF98305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="312" mass="34061" checksum="C9666E1448014642" modified="2024-11-27" version="1">MENRSMLILLLLAVSVASGSASHDPRIAALCRLSTAWRLLPDERHCGRFYVCTGDAEDAYQEFSCPLHYRFSAEQGICVPGACSEVTSSCNASTSAERIPSDCTRYRRCTPAGAYSVARCVSGSYFDTGRRACLPVAVTAAHQCSCLLPDHATVANPSDCETYYRCESGEAVLVQCPAGQYFESSVGSCLPDTTGICFERPTFPPFLTDHALALDECISTGSRLAPHSRDCGRYYVCADKRVLEMRCPRGQYFDAVHHYCALDLASECQRPESEAKTLEGTETAAIAKKPPPPPEREVAASYDKFSSKFVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FS66</accession>
    <name>A0AAU9FS66_DROMD</name>
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        <submittedName>
            <fullName evidence="6">Rho-related BTB domain-containing protein 1</fullName>
        </submittedName>
    </protein>
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        <name evidence="6" type="ORF">DMAD_06778</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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        <property type="entry name" value="Rho-related BTB domain-containing protein 2 isoform 1"/>
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        <property type="entry name" value="Rhogtpase, putative"/>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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        <property type="entry name" value="Small_GTP-bd"/>
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        <property type="entry name" value="Small_GTPase_Rho"/>
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        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHO FAMILY GTPASE"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="264"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="482"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="697"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="705"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF98693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="743" mass="84666" checksum="47897031081BB403" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FS69</accession>
    <name>A0AAU9FS69_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Odorant receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_06585</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF35">
        <property type="entry name" value="ODORANT RECEPTOR 19A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU351113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF98410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FS73</accession>
    <name>A0AAU9FS73_DROMD</name>
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        <recommendedName>
            <fullName evidence="9">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        </subcellularLocation>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
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        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF07565"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="BFF98837.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1213" mass="136487" checksum="0A77957E6F3E6151" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FS85</accession>
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        </submittedName>
    </protein>
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    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10 11">RF1</strain>
        </source>
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        <subcellularLocation>
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        </subcellularLocation>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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            <end position="712"/>
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        <location>
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        <location>
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        <location>
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            <end position="742"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF98529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="772" mass="86083" checksum="CEF160BE1EF41F3E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FS90</accession>
    <name>A0AAU9FS90_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Gamma-tubulin complex component 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_06492</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF70">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="8">
        <location>
            <begin position="284"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="7">
        <location>
            <begin position="1037"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="670"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="909"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="688"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF98301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1353" mass="153431" checksum="1B58A5B2CE0D970E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3U6</accession>
    <name>A0AAU9G3U6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ferritin</fullName>
            <ecNumber evidence="6">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_01793</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ferritin family.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="6"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3U6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01056">
        <property type="entry name" value="Euk_Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.10:FF:000002">
        <property type="entry name" value="Ferritin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014034">
        <property type="entry name" value="Ferritin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF75">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00204">
        <property type="entry name" value="FERRITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0409">Iron storage</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="7">
        <location>
            <begin position="7"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601519-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFG02225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="182" mass="20745" checksum="79CE37D81A18DDD6" modified="2024-11-27" version="1">MAMIMRQNFAKCCEKKLNDQINMELKACHQYLAMAYHCDRADVSSPGVHKFFLQASTEEREHAEKIMKYMNKRGGLIRLSAVPEPIPCFEDTLAALKYALEMELEVNQHLLDVHALAGQQNDPNLCDFIEANFLQEQVDGQKVLADYIRQLERAQSDLGEYLFDKYITAAGMHPPSVDPKGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3U7</accession>
    <name>A0AAU9G3U7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphodiesterase</fullName>
            <ecNumber evidence="9">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_02067</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Vital for female fertility. Required for learning/memory.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="9">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
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    <sequence length="1081" mass="116376" checksum="1AFDA145E69CC73C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Diptera</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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    <feature type="region of interest" description="Disordered" evidence="8">
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        <location>
            <begin position="1772"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1973"/>
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            <dbReference type="ARBA" id="ARBA00022448"/>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    <evidence type="ECO:0000256" key="9">
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            <dbReference type="Pfam" id="PF25508"/>
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        <source>
            <dbReference type="EMBL" id="BFG03188.1"/>
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    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2023" mass="225456" checksum="FDDDE093EE2EED17" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G466</accession>
    <name>A0AAU9G466_DROMD</name>
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        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_02158</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the UPL family. TOM1/PTR1 subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="BFG02728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000007">
        <property type="entry name" value="E3 ubiquitin-protein ligase HUWE1 isoform X2"/>
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    <dbReference type="FunFam" id="3.30.2410.10:FF:000004">
        <property type="entry name" value="E3 ubiquitin-protein ligase HUWE1, variant"/>
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    <dbReference type="FunFam" id="3.90.1750.10:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase UPL1"/>
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        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
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        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
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    </dbReference>
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        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="E3_Ub_ligase_DUF908"/>
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        <property type="entry name" value="E3_Ub_ligase_DUF913"/>
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        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
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    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 12" id="KW-0833">Ubl conjugation pathway</keyword>
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        </location>
    </feature>
    <feature type="domain" description="WWE" evidence="16">
        <location>
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    </feature>
    <feature type="domain" description="HECT" evidence="15">
        <location>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00034494"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG02728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="5296" mass="570920" checksum="8C60234F1D12C112" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G470</accession>
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        <recommendedName>
            <fullName evidence="1">non-specific protein-tyrosine kinase</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="15" type="ORF">DMAD_02558</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 17">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="1"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007435">
        <property type="term" value="P:salivary gland morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05068">
        <property type="entry name" value="PTKc_Frk_like"/>
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    </dbReference>
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        <property type="entry name" value="SH2_Src_Src42"/>
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    </dbReference>
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    <dbReference type="FunFam" id="3.30.200.20:FF:000053">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
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        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
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    </dbReference>
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        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH2" evidence="13">
        <location>
            <begin position="1335"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="1463"/>
            <end position="1716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="308"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1013"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1155"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
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            <begin position="248"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="457"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="471"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="630"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="746"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="788"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1013"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
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            <end position="1164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
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            <end position="1181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="1491"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG03257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1729" mass="188471" checksum="7C6A06D8D6679463" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G476</accession>
    <name>A0AAU9G476_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Amyloid-beta-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_01871</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the APP family.</text>
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    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:neuronal cell body"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:heparin binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.770:FF:000002">
        <property type="entry name" value="beta-amyloid-like protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.570.10:FF:000002">
        <property type="entry name" value="beta-amyloid-like protein isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.770">
        <property type="entry name" value="Amyloid precursor protein, E2 domain"/>
        <property type="match status" value="1"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <end position="1451"/>
        </location>
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            <end position="1548"/>
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            <end position="1864"/>
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        <location>
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            <end position="2062"/>
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            <begin position="81"/>
            <end position="100"/>
        </location>
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        <location>
            <begin position="496"/>
            <end position="524"/>
        </location>
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            <end position="512"/>
        </location>
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        </location>
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            <begin position="2223"/>
            <end position="2236"/>
        </location>
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        </source>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00061621"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG02349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2303" mass="255073" checksum="B1891702DDD1C302" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G480</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="3" type="ORF">DMAD_01962</name>
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    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Brachycera</taxon>
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        </lineage>
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    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
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            <end position="176"/>
        </location>
    </feature>
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            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
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        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG02467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="176" mass="19961" checksum="630A9562172662EB" modified="2024-11-27" version="1">MNPNSHMCVPLFLLFALILASLWGFFNREELFDAYRKQLHGEAKHGKISRLDFINMFAQLNEHTAAVQPLQLAAAKNISMGNVTTIQNLTQTTDLEDKDEDAEVLVLEDYQQEDEDDDEEPQATGSTENPLIDLPEVVQLIQLPMSTDSNLKREFASDNANPYIEGINEHLELFCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <name evidence="10" type="ORF">DMAD_02290</name>
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    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
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    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="217"/>
            <end position="385"/>
        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="BFG02914.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="392" mass="44251" checksum="4471F68490807E3E" modified="2024-11-27" version="1">MACLNTLKQEIKTLEKIFPKNHERFQILNSSVDELLCRFIDKNGKRYDIHANITETYPSSPPVWFAESEETSVTNAVQILSNTNGRDNHVINQVGILLRELCRLHNVPLPPDVDNLTLPLQTPPPSASPLRCEQRKMGGGPHGGNEETDSDQDEIEDPIGESEQESEGDEDLPLEMDDVRSTNKKDDMEVEHLATLERLRQSQRQDYLKGSVSGSVQATDRLMKELRDIYRSDAFKKNMYLIELVNDSIYEWNIRLKSVDPDSPLHSDLLMLKEKEGKDSILLNILFKETYPFEPPFVRVVHPIISGGYVLIGGAICMELLTKQGWSSAYTVEAVIMQIAATLVKGKARIQFGATKALTQGQYSLARAQQSFKSLVQIHEKNGWFTPPKEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDW1</accession>
    <name>A0AAU9GDW1_DROMD</name>
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        <submittedName>
            <fullName evidence="18">Muscarinic acetylcholine receptor DM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_04341</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Postsynaptic cell membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDW1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016907">
        <property type="term" value="F:G protein-coupled acetylcholine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007197">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15301">
        <property type="entry name" value="7tmA_mAChR_DM1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000038">
        <property type="entry name" value="Muscarinic acetylcholine receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000288">
        <property type="entry name" value="Muscarinic acetylcholine receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000995">
        <property type="entry name" value="Musac_Ach_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF265">
        <property type="entry name" value="MUSCARINIC ACETYLCHOLINE RECEPTOR DM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="MUSCARINICR"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9 14 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="11 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="97"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="137"/>
            <end position="166"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="16">
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            <begin position="178"/>
            <end position="196"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
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            <begin position="217"/>
            <end position="237"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="263"/>
            <end position="285"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="710"/>
            <end position="728"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="748"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="117"/>
            <end position="764"/>
        </location>
    </feature>
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        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
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        <location>
            <begin position="298"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="439"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="614"/>
            <end position="635"/>
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    </feature>
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        <location>
            <begin position="304"/>
            <end position="314"/>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFG05651.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="797" mass="85392" checksum="7629F6CEF2263198" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDW2</accession>
    <name>A0AAU9GDW2_DROMD</name>
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        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
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        <name evidence="18" type="ORF">DMAD_04432</name>
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    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
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            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
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    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005524">
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF7084"/>
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        <property type="entry name" value="KA1/Ssp2_C"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="UBA_SIK1/2/3"/>
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    </dbReference>
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        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SIK3"/>
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    </dbReference>
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        <property type="entry name" value="Pkinase"/>
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        <property type="entry name" value="UBA_SIK3"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="47"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="17">
        <location>
            <begin position="1423"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="663"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="784"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1245"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1366"/>
            <end position="1416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="675"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1252"/>
            <end position="1264"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1277"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1294"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1306"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1390"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFG05772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1481" mass="158963" checksum="2E0C9D0B6C84B596" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDW3</accession>
    <name>A0AAU9GDW3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Heterogeneous nuclear ribonucleoprotein U-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_04361</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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                <person name="Yamamoto D."/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF22248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF22249"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG06213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="880" mass="99053" checksum="E72288FE8F662D78" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDX2</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">DMAD_04458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
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        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG05812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="155" mass="17439" checksum="DB3C93302E0DDE91" modified="2024-11-27" version="1">MWNLICSGRYPTDVLIRYGGRLVAPGDPYAVFQSENEHVDFLAVFRSKSIVLCKSLRIKSMQTFECIDGDGATRLIEIGHSHLVCVEYAFRLVDELVEHCTNAKVDPNKFSALYYANIEIVLNKFKTSCGLALSSNILLVIASALVLIFVILHWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDX4</accession>
    <name>A0AAU9GDX4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_04921</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:intracellular cyclic nucleotide activated cation channel complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyclic nucleotide gated channel alpha 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000100">
        <property type="entry name" value="Cyclic nucleotide-gated cation channel"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
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        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638:SF4">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="459"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="585"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="689"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="199"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="866"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1030"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="980"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="200"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="214"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="331"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="345"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="866"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="941"/>
            <end position="955"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG06404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1052" mass="116434" checksum="54643870A1ECE28A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDX8</accession>
    <name>A0AAU9GDX8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_13659</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000064">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000081">
        <property type="entry name" value="Zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
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    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45891">
        <property type="entry name" value="ZINC FINGER HOMEOBOX PROTEIN"/>
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    </dbReference>
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    <sequence length="3104" mass="340275" checksum="DCB8D318B03322B8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="11">May be an inorganic phosphate cotransporter.</text>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="495" mass="54641" checksum="BA0EC785A3257CB2" modified="2024-11-27" version="1">MTTALTMVKTGEKLFGVRHVQCILCFFCLAMCYAWRVNLSVALVAMSEPPSVNVTENDTASAVPDDDDDDFGIFKAEYYPFDEGQKSYMLSSFFWGYIVTQVPGGYIAQRYGAKMLLLAGLAAAAILTIISPLALKLGGWIALCAVRFTMGLTQGAVHPATHALLSKWSPANERGMLGTICYSGAQFGTVVMLATSGYIADSFMGWPSIFYLGGACGFVWVVFWYVFSSSTPEEHRFISPEELKFIEESRSDGKMQSADSLAPTPWLPIFTSMPFLSLLVVHCTHMFGFWTLLTQIPSYMKNIYGIDIKESALLSSLPYTVMLLLSFFFVWLSKVLQRNKSLSLSFNRKFFNSIGHWIPMCSLIALGYVPKEDNLLAVVLLTLTVGISAATYLGFQVNHIDLSPNYAGTLMGLTNGAANIMSGIAPLVVGQIVQDPKNVNEWRLVFFLAAAAYFIGNLLFVVFGRTEVQWWDSPEQSVDDVEQGVPLSPNGQDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GE85</accession>
    <name>A0AAU9GE85_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">SPARC-related modular calcium-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_04540</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GE85"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16234">
        <property type="entry name" value="EFh_SPARC_SMOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051950">
        <property type="entry name" value="Dev_reg/Prot_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019577">
        <property type="entry name" value="SPARC/Testican_Ca-bd-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352:SF30">
        <property type="entry name" value="FI05255P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352">
        <property type="entry name" value="SECRETED MODULAR CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10591">
        <property type="entry name" value="SPARC_Ca_bdg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043930707" evidence="10">
        <location>
            <begin position="19"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="13">
        <location>
            <begin position="29"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="12">
        <location>
            <begin position="78"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="218"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="12">
        <location>
            <begin position="409"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="132"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="317"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="598"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="317"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="598"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="447"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFG05922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="630" mass="70876" checksum="F16A79140ABA62FC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GE87</accession>
    <name>A0AAU9GE87_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Semaphorin-2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_04397</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GE87"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:semaphorin receptor binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04979">
        <property type="entry name" value="Ig_Semaphorin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11238">
        <property type="entry name" value="Sema_2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000369">
        <property type="entry name" value="semaphorin-2A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001506">
        <property type="entry name" value="semaphorin-2A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000024">
        <property type="entry name" value="semaphorin-2A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF90">
        <property type="entry name" value="SEMAPHORIN 2B, ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043728719" description="Semaphorin-2A" evidence="13">
        <location>
            <begin position="24"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="15">
        <location>
            <begin position="13"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="527"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GE89</accession>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Yamamoto D."/>
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        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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</entry>
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            <taxon>Diptera</taxon>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <strain evidence="6 7">RF1</strain>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1341"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1278"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1365"/>
            <end position="1398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFG05739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1517" mass="167915" checksum="03BB216C65A80484" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GE97</accession>
    <name>A0AAU9GE97_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_04461</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GE97"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 7" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG05816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="393" mass="45429" checksum="2ACFF4867047589A" modified="2024-11-27" version="1">MVDLVKFWVRIGYLYARFTGTLNFEVDLKTGRTRVTRGATIYSAFANLFLISILAHHFGPSKIKCELWKDISSLHESVFMIVSWLRVSCALAALASRWYHRRRYIRLISCFRRLFLQNPEVMRLCRRGFISKCFVTSVAEIMQFLVSILTVWDKLTIALLLGIWGVMTVTAIMNVAITQYFFALGNARGHYILINRKLRALLGEAQSLGPRRKRRNGVFITKCCFLADRLDEIAQTQSEVQALIEGMSKIYGLQILCLCLTYYLTCVANIYILFSLYKYNGMTQSWSKLGMVAAAIFFIFYHIDCVINSYNVFYLLDAHQEMHKLLEQRNLFPGALDERLESAFDSFELSLARNPLNLKCFGLFRLNRYSAFDVGNSILINSVLLIQYDMQNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GE98</accession>
    <name>A0AAU9GE98_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transcriptional repressor CTCF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_04495</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GE98"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002350">
        <property type="entry name" value="CG10321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF13">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="187"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG05855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="309" mass="33362" checksum="F8296034A683F6C6" modified="2024-11-27" version="1">MGTPTLSVSSPSPTPKRAKRGNQSTSTSASGVTLEVCNHQSSPPAISSKLAAANSRQLVQTASVIAAAGADDNYEIDANLVTEFIKQHTSPLGSGRYVCHLCSTEFRQFKGLQNHMHSHTNWIRANCKKQPQCQICLKSFKGPGMLRMHMKTHDAETNTPVCTICNRTFKSKAILYRHRQTHQQRAYCCGVSNCRKNFSSAANLRWHVERKHPDVIDPFYKCGECSTLYDSIDGLQQHVENTDHSEAQISDTTSNSNAAFSGAVSIVSNGTDMSNMIAAAPGGLGTGAPGDTHAVVVGSSGEVYFVTQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EPA5</accession>
    <name>A0AAU9EPA5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Very low-density lipoprotein receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_07725</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EPA5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000105">
        <property type="entry name" value="Lipophorin receptor 1, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000002">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000011">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="low-density lipoprotein receptor-related protein 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000008">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
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        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
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    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
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        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MEGALIN, ISOFORM A"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
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        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="8"/>
    </dbReference>
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        <property type="entry name" value="LY"/>
        <property type="match status" value="5"/>
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        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
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        <property type="match status" value="8"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="8"/>
    </dbReference>
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        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="834"/>
            <end position="858"/>
        </location>
    </feature>
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        <location>
            <begin position="68"/>
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        </location>
    </feature>
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        <location>
            <begin position="357"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="360"/>
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    </feature>
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        <location>
            <begin position="398"/>
            <end position="438"/>
        </location>
    </feature>
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        <location>
            <begin position="401"/>
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        </location>
    </feature>
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        <location>
            <begin position="484"/>
            <end position="527"/>
        </location>
    </feature>
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        <location>
            <begin position="528"/>
            <end position="570"/>
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    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="16">
        <location>
            <begin position="571"/>
            <end position="614"/>
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    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="16">
        <location>
            <begin position="615"/>
            <end position="659"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="710"/>
            <end position="753"/>
        </location>
    </feature>
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        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="33"/>
            <end position="51"/>
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    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="69"/>
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    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="76"/>
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    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="129"/>
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    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="148"/>
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    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="155"/>
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    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="167"/>
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    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
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    <feature type="disulfide bond" evidence="15">
        <location>
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    <feature type="disulfide bond" evidence="15">
        <location>
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    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="281"/>
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    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="293"/>
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    </feature>
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        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00009939"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00179"/>
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    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF88811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="916" mass="101903" checksum="2921C432EF182099" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EPA7</accession>
    <name>A0AAU9EPA7_DROMD</name>
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        <submittedName>
            <fullName evidence="20">Very low-density lipoprotein receptor</fullName>
        </submittedName>
    </protein>
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        <name evidence="20" type="ORF">DMAD_07725</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
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        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="entry name" value="Lipophorin receptor 1, isoform K"/>
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    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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</entry>
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</entry>
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            <taxon>Insecta</taxon>
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            <taxon>Diptera</taxon>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
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        </reaction>
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    <comment type="similarity">
        <text evidence="3 13">Belongs to the eukaryotic diacylglycerol kinase family.</text>
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    <sequence length="1576" mass="172438" checksum="F4D761AB775CCBD6" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXL6</accession>
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        <submittedName>
            <fullName evidence="15">Myosin heavy chain 95F</fullName>
        </submittedName>
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        <name evidence="15" type="ORF">DMAD_09580</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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        <text evidence="2 11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
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        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR036114">
        <property type="entry name" value="MYSc_Myo6"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="SH3_Myosin"/>
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        <property type="entry name" value="MYOSIN"/>
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        <property type="entry name" value="UNCONVENTIONAL MYOSIN-VI"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myosin-VI_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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        <property type="entry name" value="IQ"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3_LIKE"/>
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    <keyword evidence="7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8 11" id="KW-0518">Myosin</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="21"/>
            <end position="72"/>
        </location>
    </feature>
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        <location>
            <begin position="75"/>
            <end position="784"/>
        </location>
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    <feature type="region of interest" description="Actin-binding" evidence="11">
        <location>
            <begin position="664"/>
            <end position="686"/>
        </location>
    </feature>
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        <location>
            <begin position="953"/>
            <end position="996"/>
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        <location>
            <begin position="1251"/>
            <end position="1273"/>
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        <location>
            <begin position="169"/>
            <end position="176"/>
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        <ligand>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF91255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1273" mass="145718" checksum="BBCA63257C4AEF1B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXN1</accession>
    <name>A0AAU9EXN1_DROMD</name>
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        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="11" type="ORF">DMAD_09589</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="1 13">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
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    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:cell migration"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
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    </dbReference>
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        <property type="entry name" value="UBIQUITINYL HYDROLASE 1"/>
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    </dbReference>
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    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
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        <location>
            <begin position="1705"/>
            <end position="2098"/>
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    </feature>
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    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="9"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
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        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1109"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1204"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="2615"/>
            <end position="2624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="2633"/>
            <end position="2643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="2644"/>
            <end position="2653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="2661"/>
            <end position="2674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="2716"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="2730"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="2768"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="2818"/>
            <end position="2844"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF91270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2852" mass="318039" checksum="421A18D781D4E16E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXP8</accession>
    <name>A0AAU9EXP8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Neurexin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_09016</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000015">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.60.120.200:FF:000005">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="neurexin-1 isoform X3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="neurexin-1 isoform X4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="neurexin-1 isoform X4"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
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    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF89">
        <property type="entry name" value="NEUREXIN 1, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 8" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1723"/>
            <end position="1746"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="11">
        <location>
            <begin position="120"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="324"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="11">
        <location>
            <begin position="367"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="11">
        <location>
            <begin position="569"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="757"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="11">
        <location>
            <begin position="799"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="11">
        <location>
            <begin position="997"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="1175"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="11">
        <location>
            <begin position="1216"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1426"/>
            <end position="1685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1765"/>
            <end position="1861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1477"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1513"/>
            <end position="1562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1563"/>
            <end position="1628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1660"/>
            <end position="1674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1675"/>
            <end position="1685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1796"/>
            <end position="1810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1811"/>
            <end position="1834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1852"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF90510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1861" mass="201660" checksum="E0CBEB33EB4FC91F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXQ4</accession>
    <name>A0AAU9EXQ4_DROMD</name>
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        <recommendedName>
            <fullName evidence="13">Signal transducer and transcription activator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_09415</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="12">Forms a homodimer or a heterodimer with a related family member.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transcription factor STAT family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF91037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09919">
        <property type="entry name" value="SH2_STAT_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14786">
        <property type="entry name" value="STAT_CCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14801">
        <property type="entry name" value="STAT_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000095">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.630:FF:000003">
        <property type="entry name" value="Signal transducer and transcription activator 6"/>
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    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.20">
        <property type="entry name" value="STAT transcription factor, all-alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.630">
        <property type="entry name" value="STAT transcription factor, DNA-binding domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.532.10">
        <property type="entry name" value="STAT transcription factor, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001217">
        <property type="entry name" value="STAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048988">
        <property type="entry name" value="STAT_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036535">
        <property type="entry name" value="STAT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013800">
        <property type="entry name" value="STAT_TF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015988">
        <property type="entry name" value="STAT_TF_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013801">
        <property type="entry name" value="STAT_TF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012345">
        <property type="entry name" value="STAT_TF_DNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013799">
        <property type="entry name" value="STAT_TF_prot_interaction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11801">
        <property type="entry name" value="SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01017">
        <property type="entry name" value="STAT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02864">
        <property type="entry name" value="STAT_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02865">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21354">
        <property type="entry name" value="STAT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00964">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47655">
        <property type="entry name" value="STAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SH2" evidence="16">
        <location>
            <begin position="584"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="728"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF91037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="791" mass="88653" checksum="49106BD36210E00B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXQ5</accession>
    <name>A0AAU9EXQ5_DROMD</name>
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        <recommendedName>
            <fullName evidence="7">Guanine nucleotide-binding protein-like 3 homolog</fullName>
        </recommendedName>
    </protein>
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        <name evidence="11" type="ORF">DMAD_09895</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play a role in regulating cellular proliferation.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="BFF91668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:regulation of developmental process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GTP-bd"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF30">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3 HOMOLOG"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="548" mass="61842" checksum="F12A68E0CE664A2C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EY04</accession>
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        <submittedName>
            <fullName evidence="14">Serine-rich adhesin for platelets</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_10062</name>
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    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000656">
        <property type="entry name" value="Zinc finger protein 541"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
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    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR16089:SF40">
        <property type="entry name" value="SUPPRESSOR OF ACTIVATED EGL-4 PROTEIN 1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="match status" value="3"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
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    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
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        <location>
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="346"/>
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="374"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
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            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="ELM2" evidence="12">
        <location>
            <begin position="1636"/>
            <end position="1723"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="13">
        <location>
            <begin position="1726"/>
            <end position="1777"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="1800"/>
            <end position="1829"/>
        </location>
    </feature>
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        <location>
            <begin position="145"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="232"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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            <end position="1109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1573"/>
            <end position="1593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1828"/>
            <end position="1852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1896"/>
            <end position="1934"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="283"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
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        </location>
    </feature>
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        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
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        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF91871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1949" mass="205657" checksum="3968241A7E63DF13" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EY06</accession>
    <name>A0AAU9EY06_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Peripheral plasma membrane protein CASK</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_09697</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAGUK family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EY06"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019098">
        <property type="term" value="P:reproductive behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10831">
        <property type="entry name" value="PDZ_CASK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12081">
        <property type="entry name" value="SH3_CASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14094">
        <property type="entry name" value="STKc_CASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000146">
        <property type="entry name" value="MAGUK p55 subfamily member 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000051">
        <property type="entry name" value="Peripheral plasma membrane protein CASK isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000062">
        <property type="entry name" value="peripheral plasma membrane protein CASK isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000016">
        <property type="entry name" value="peripheral plasma membrane protein CASK isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035473">
        <property type="entry name" value="CASK_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 16" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="12"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="L27" evidence="21">
        <location>
            <begin position="343"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="L27" evidence="21">
        <location>
            <begin position="402"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="20">
        <location>
            <begin position="494"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="581"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="19">
        <location>
            <begin position="728"/>
            <end position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00071925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="BFF91405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="915" mass="102352" checksum="41B4A129C6441474" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EY13</accession>
    <name>A0AAU9EY13_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GILT-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_08460</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GILT family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EY13"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016671">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004911">
        <property type="entry name" value="Interferon-induced_GILT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234">
        <property type="entry name" value="GAMMA-INTERFERON INDUCIBLE LYSOSOMAL THIOL REDUCTASE GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234:SF68">
        <property type="entry name" value="GH19763P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03227">
        <property type="entry name" value="GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043986804" evidence="3">
        <location>
            <begin position="24"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF89784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="217" mass="24117" checksum="C5986679B014F12E" modified="2024-11-27" version="1">MQKFPTALSMSLLLGLMLTLSCSSELVGEPREKRQADKLHITLLYESLCPDSRNFMHQLGPVHEELQQYMDIQLVPFGKSRSEQSGAIFHCQHGPAECEGNRVQSCVISSTANQSAQVKFVVCQMFALDSSRADQCASEAGLRTDVDHCADSESGTKLQLQAELVTKLYQPSFIPTIVYNGVFNQQLQDHSLRDFRGTVCHLLRQQNLLPSGSPVCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EY20</accession>
    <name>A0AAU9EY20_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">BK channel</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Maxi K channel</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_10072</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. Slo sub-subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EY20"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000035">
        <property type="entry name" value="Calcium-activated potassium channel slowpoke"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001832">
        <property type="entry name" value="Calcium-activated potassium channel slowpoke-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000015">
        <property type="entry name" value="Calcium-activated potassium channel subunit alpha-1 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01449">
        <property type="entry name" value="BKCHANNELA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="19">
        <location>
            <begin position="356"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="19">
        <location>
            <begin position="852"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="657"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="784"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1192"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="784"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF91888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1222" mass="135643" checksum="7FC61933ECCC9DF2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EY22</accession>
    <name>A0AAU9EY22_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glycerol-3-phosphate acyltransferase 1 mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_09999</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GPAT/DAPAT family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EY22"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07993">
        <property type="entry name" value="LPLAT_DHAPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022284">
        <property type="entry name" value="GPAT/DHAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045520">
        <property type="entry name" value="GPAT/DHAPAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041728">
        <property type="entry name" value="GPAT/DHAPAT_LPLAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028354">
        <property type="entry name" value="GPAT_PlsB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563:SF23">
        <property type="entry name" value="BCDNA.GH07066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19277">
        <property type="entry name" value="GPAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500064">
        <property type="entry name" value="GPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000437">
        <property type="entry name" value="GPAT_DHAPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="8">
        <location>
            <begin position="192"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="634"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF91788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="788" mass="88729" checksum="FC828F96211250C1" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EY36</accession>
    <name>A0AAU9EY36_DROMD</name>
    <protein>
        <submittedName>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
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                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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            <taxon>Pterygota</taxon>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <dbReference type="RuleBase" id="RU004515"/>
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            <dbReference type="EMBL" id="BFF89339.1"/>
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            <dbReference type="Proteomes" id="UP001500889"/>
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    <sequence length="284" mass="32921" checksum="EE601141458E2603" modified="2024-11-27" version="1">MDAIKKKMQAMKLEKDNAIDKADTCENQAKDANSRADKLNEEVRDLEKKFVQVEVDLVTAKEQLEKANAELEEKEKLLTSTESEVATQNRKVQQIEEDLEKSEERSTTAQQKLLEATQSADENNRMCKVLENRSQQDEERMDQLTNQLKEARMLAEDADTKSDEVSRKLAFVEDELEVAEDRVRSGEAKIMELEEELKVVGNSLKSLEVSEEKANQRVEEFKREMKTLAIKLKEAEQRAEHAEKQVKRLQKEVDRLEDRLFNEKEKYKAICDDLDQTFAELTGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EY48</accession>
    <name>A0AAU9EY48_DROMD</name>
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        <recommendedName>
            <fullName evidence="9">Ecdysteroid-phosphate phosphatase</fullName>
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    </protein>
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        <name evidence="15" type="ORF">DMAD_09538</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15 16">RF1</strain>
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        <reaction evidence="7">
            <text>2-deoxyecdysone 22-phosphate + H2O = 2-deoxyecdysone + phosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:19566"/>
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            <dbReference type="ChEBI" id="CHEBI:147386"/>
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    <comment type="catalytic activity">
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            <text>20-hydroxyecdysone 22-phosphate + H2O = 20-hydroxyecdysone + phosphate</text>
            <dbReference type="Rhea" id="RHEA:63580"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
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            <dbReference type="ChEBI" id="CHEBI:147382"/>
        </reaction>
    </comment>
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        <reaction evidence="8">
            <text>ecdysone 22-phosphate + H2O = ecdysone + phosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16688"/>
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            <dbReference type="ChEBI" id="CHEBI:147380"/>
        </reaction>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="C:cytosol"/>
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        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
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        <property type="entry name" value="Phosphoglycerate mutase-like"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="UBA"/>
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    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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            <begin position="16"/>
            <end position="60"/>
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    <sequence length="719" mass="81054" checksum="5BA6CA56E8CD1A3E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0W0</accession>
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            <fullName evidence="4">WASP family protein member</fullName>
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            <taxon>Muscomorpha</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
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            <strain evidence="2 3">RF1</strain>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="14 15">RF1</strain>
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    </comment>
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        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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            <end position="1392"/>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50923"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51220"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF92351.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9L5</accession>
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            <fullName evidence="8">Pre-mRNA-processing factor 39</fullName>
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        </alternativeName>
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        <name evidence="12" type="ORF">DMAD_10465</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
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        <text evidence="1">Involved in pre-mRNA splicing.</text>
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        <subcellularLocation>
            <location evidence="2">Nucleus</location>
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        <property type="entry name" value="PRE-MRNA PROCESSING PROTEIN PRP39-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204:SF5">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067962"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00080852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF92395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1041" mass="116674" checksum="619606AE8AE31BDA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9L9</accession>
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        <submittedName>
            <fullName evidence="11">ABC transporter G family member 23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_10453</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
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            <authorList>
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        <location>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Yamamoto D."/>
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        <source>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <alternativeName>
            <fullName evidence="9">PRP39 homolog</fullName>
        </alternativeName>
    </protein>
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        <name evidence="12" type="ORF">DMAD_10465</name>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Pre-mRNA-processing factor 39"/>
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    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="DnaJ_domain"/>
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</entry>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <property type="project" value="UniProtKB-KW"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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        </source>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="765" mass="84474" checksum="E58F433116CFC241" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
    </comment>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
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</entry>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
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        <source>
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    </evidence>
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</entry>
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        <name evidence="3" type="ORF">DMAD_10512</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        </lineage>
    </organism>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
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    </dbReference>
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    </feature>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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    <feature type="region of interest" description="Disordered" evidence="1">
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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</entry>
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            <authorList>
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</entry>
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            <authorList>
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                <person name="Yamamoto D."/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="482"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF92987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="681" mass="76400" checksum="46B3EA754178DDE3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJI3</accession>
    <name>A0AAU9FJI3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Chitinase-like protein Idgf3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_13244</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 18 family. IDGF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJI3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02873">
        <property type="entry name" value="GH18_IDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000007">
        <property type="entry name" value="chitinase-like protein Idgf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000071">
        <property type="entry name" value="Imaginal disc growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015520">
        <property type="entry name" value="IDGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF235">
        <property type="entry name" value="CHITINASE-LIKE PROTEIN IDGF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043369980" evidence="8">
        <location>
            <begin position="25"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="9">
        <location>
            <begin position="26"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF95942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="442" mass="49280" checksum="7D857856E98EF4BF" modified="2024-11-27" version="1">MRSSLWLGLGLSVMLLASFSSVNAAPNLVCFYDSQGFQRQGLAQFTMTDVELALQFCTHIVYGYAGVNADNSELQSINKRLDYEQRHLAQITALKDRYPHIKFLLSVGGDADQNEGNQYIKLLESGQQGHRRFIESARDVVRRFNFDGLDLALQLPRNKPRKVHGDVGSAWKSFKKFFTGDYIVDEDSETHKSQLTALIKDLSSAFKDNNLLLSLTVLPNVNSSWYYDAPAIAPSLDFINLGTFDFLTPQRNPEEADFSAPLYEAFGQNRLGHYNVNFQLEHWLLQRVPANKLNIGIATYGRAWKLSSGSGDTGMPVVTATEGAAPAGRQSKKEGMLNWAEICQLMPNPSNANAKGADAPVRRVLDPTKRYGSYAFRAADSNGEHGLWISYDDPDSASSKASYVRSKNLGGVALFDLTQDDFRGQCTTDRFPMLRAIKYRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJJ1</accession>
    <name>A0AAU9FJJ1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ABC transporter G family member 21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_13344</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJJ1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001276">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF63">
        <property type="entry name" value="EARLY GENE AT 23, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="494"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="525"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="613"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="281"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF96071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="644" mass="73878" checksum="8AC391B28BEAD399" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJN7</accession>
    <name>A0AAU9FJN7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Coiled-coil and C2 domain-containing protein 1-like</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Lethal (2) giant discs 1 protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13168</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="16 17">RF1</strain>
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        <text evidence="11">Interacts (via DM14 domains 1 and 3) with shrb; the interaction is direct and blocks access to the surface involved in shrb polymerization. This interaction may be required for the ESCRT-III complex role in multivesicular body formation.</text>
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            <location evidence="10">Apicolateral cell membrane</location>
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            <location evidence="2">Cytosol</location>
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    <comment type="similarity">
        <text evidence="4">Belongs to the CC2D1 family.</text>
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        <property type="entry name" value="COILED-COIL AND C2 DOMAIN-CONTAINING PROTEIN 1-LIKE"/>
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        <property type="entry name" value="COILED-COIL AND C2 DOMAIN-CONTAINING PROTEIN 1-LIKE"/>
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    <sequence length="816" mass="88626" checksum="759888CC744A6130" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <dbReference type="EMBL" id="BFF95989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2368" mass="260059" checksum="7323AF49F24AFC0E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJQ7</accession>
    <name>A0AAU9FJQ7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Fez family zinc finger protein erm</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13280</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJQ7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000103">
        <property type="entry name" value="FEZ family zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000251">
        <property type="entry name" value="FEZ family zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000227">
        <property type="entry name" value="fez family zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000164">
        <property type="entry name" value="Fez family zinc finger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000194">
        <property type="entry name" value="Fez family zinc finger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="391"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="419"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="447"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="475"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="242"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="519"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="287"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="526"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="604"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF95993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="639" mass="68502" checksum="6CAC6D746A248978" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJS0</accession>
    <name>A0AAU9FJS0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_13291</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJS0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008586">
        <property type="term" value="P:imaginal disc-derived wing vein morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045879">
        <property type="term" value="P:negative regulation of smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045807">
        <property type="term" value="P:positive regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04021">
        <property type="entry name" value="C2_E3_ubiquitin_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000068">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000084">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000221">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000002">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000002">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024928">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF429">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SU(DX)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
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</entry>
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                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="529"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="638"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="703"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF95834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="825" mass="93999" checksum="59855D7C73DC9BFE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJT7</accession>
    <name>A0AAU9FJT7_DROMD</name>
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        <submittedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 4E-binding protein Mextli</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_13313</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJT7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034518">
        <property type="term" value="C:RNA cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008190">
        <property type="term" value="F:eukaryotic initiation factor 4E binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901190">
        <property type="term" value="P:regulation of formation of translation initiation ternary complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22454">
        <property type="entry name" value="KH-I_Mextli_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000048">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000072">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040160">
        <property type="entry name" value="Mxt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20849">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-BINDING PROTEIN MEXTLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20849:SF2">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-BINDING PROTEIN MEXTLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="228"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="307"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="504"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="514"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="535"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF96034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="638" mass="68771" checksum="8D305F790215914A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJU3</accession>
    <name>A0AAU9FJU3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Kinesin-like protein KIN-7L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_13149</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJU3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF75">
        <property type="entry name" value="CENTROMERE-ASSOCIATED PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="2"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="400"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="732"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="74"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF95825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="905" mass="102798" checksum="0EE2E2F0DC9411E2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJU5</accession>
    <name>A0AAU9FJU5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Voltage-dependent L-type calcium channel subunit beta-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_13155</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calcium channel beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJU5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11863">
        <property type="entry name" value="SH3_CACNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003279">
        <property type="entry name" value="Ca2+-channel-protein-beta-subunit, isoform M"/>
        <property type="match status" value="1"/>
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            <begin position="123"/>
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        <location>
            <begin position="180"/>
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            <dbReference type="SAM" id="SignalP"/>
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            <dbReference type="PROSITE" id="PS50940"/>
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            <dbReference type="EMBL" id="BFF96103.1"/>
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    </evidence>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FK16</accession>
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        <recommendedName>
            <fullName evidence="6">Lethal(2) giant larvae protein</fullName>
        </recommendedName>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08366"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF96098.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1090" mass="119042" checksum="5119E2577E031BE8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FK24</accession>
    <name>A0AAU9FK24_DROMD</name>
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        <submittedName>
            <fullName evidence="4">Transport and Golgi organization protein 6</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ORF">DMAD_13215</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the Tango6 family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF95911.1"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="651"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase II assembly factor Rtp1 C-terminal" evidence="2">
        <location>
            <begin position="850"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF95911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="923" mass="105076" checksum="70D95880BE534CC2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FK41</accession>
    <name>A0AAU9FK41_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SKI/SNO/DAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_13245</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DACH/dachshund family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FK41"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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</entry>
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                <person name="Llopart A."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FK50</accession>
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        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="376"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="404"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="432"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF96169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="457" mass="51518" checksum="741D4D8EFB9B152D" modified="2024-11-27" version="1">MIKSTTNIQEQRLPRPEDQPQQVHPPQPPPPPPPTHQVAAVIANMDTLKTAFLPNLSMDPNVHVSPHYCPMCHQQFERAQHVTEHMQLCHGITLNAQHQQQQQQQQQQQQQQLKPSYSCINCDEKFGSAVDLDEHQRMTHQATAFLARCLVCSIYGVHSATPNPNEYKCTQCGSVCTTSMLAAGQQAFMEQQEAPVTPDELPAIAPRDMRLTPEEQHHQQQQQLQQEHHHQQQELLEQQQRELQEHQQQQHHQDDLSGDQVALKVPPLTVKLNKNSNGGSVAHPQVIIKEEPLSLSDSGDVVNAVPVYAIQANPGVQAPAATSVLVSTRIVTADQAHKIRHKCPDCPKTFKTLGTLAMHRKIHTGEADATPKERPYTCSYCGKSFTQSNTLKQHTRIHTGEKPFHCGYCEKSFSVKDYLTKHIRTHTGEKPYTCPYCDKRFTQRSALTVHTTKLHPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FK67</accession>
    <name>A0AAU9FK67_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Geranylgeranyl transferase type-1 subunit beta</fullName>
            <ecNumber evidence="4">2.5.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Geranylgeranyl transferase type I subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Type I protein geranyl-geranyltransferase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_13411</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heterodimer of FNTA and PGGT1B. PGGT1B mediates interaction with substrate peptides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.59" evidence="4"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FK67"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005953">
        <property type="term" value="C:CAAX-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004662">
        <property type="term" value="F:CAAX-protein geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02895">
        <property type="entry name" value="GGTase-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000005">
        <property type="entry name" value="Geranylgeranyl transferase type-1 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041960">
        <property type="entry name" value="GGTase_I_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF4">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-1 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="16">
        <location>
            <begin position="13"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00065714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00078363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF96158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="399" mass="44231" checksum="7D15814A4B949675" modified="2024-11-27" version="1">MEDKTEPEPVLLSKHAKNLLRFLNMLPARMASHDNTRSTIVFFAVCGLDVLNSLNLVPPQLRQDIIDWIYGGLVVPRDNEKRCGGFMGCRAMVPLTEDADVLACMRKYQWGHLAMTYTSIAVLVTLGDDLARLDRKSIVDGVAAVQRPEGSFSACIDGSEDDMRFVYCAATICHMLDYWGDVDKEAMFQFIAKSLRYDYGFSQELEGEAHGGTTFCALAALQLSGQLHRLDAATVERIKRWLIFRQMDGFQGRPNKPVDTCYSFWIGASLCILDGFELTDYAKNREYILSTQDTLVGGFAKWPQATPDPFHTYLGLCGLAFTGEPGLSAVMPSLNMSMAAYVHLQHLHEVWRSKSKEGSEDNDLAISSAVQHQLKLTKDGEAIATTTATTSTSPLISAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRZ2</accession>
    <name>A0AAU9FRZ2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Gustatory receptor for sugar taste 64b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_06688</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family. Gr5a subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRZ2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033041">
        <property type="term" value="F:sweet taste receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009318">
        <property type="entry name" value="Gustatory_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21421">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21421:SF35">
        <property type="entry name" value="GUSTATORY RECEPTOR FOR SUGAR TASTE 64B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06151">
        <property type="entry name" value="Trehalose_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038981">
        <property type="entry name" value="GRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF98548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="352" mass="41352" checksum="2C5A6BDA864ABD4C" modified="2024-11-27" version="1">MNICEFGAVLNYVGQVAINFHNSSTLSLYVVCLLEHFFFWRLAVQWPSIMRTWHSVEQLFLRVPYRFYGEYRMKRRIYIVCSVVMLSALAEHCLLLTNSFHLSNMERTQCKINVTYLESIYRWERPHLYIILPYHMWMLPFLEWINETIAYPRSFTDCFIMCIGIGLAARFHQLYRRIAAVHRKVMPAVFWTEVREHYLALKRLVRLLDAAIAPLVLLAFGNNMSFICFQLFNSFKNIGVDFMVMLAFWYSLVFAVMRTLLTIFVASSINDIERKIVTALRDVPSRAWSIEVQRFSEQLGHDMTALSGSGFFYLTRSLVLAMGTTIITYELMISDVINQGGIRQKTQYCREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRZ7</accession>
    <name>A0AAU9FRZ7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-phosphoinositide-dependent protein kinase 1</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_06578</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRZ7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901701">
        <property type="term" value="P:cellular response to oxygen-containing compound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048638">
        <property type="term" value="P:regulation of developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01262">
        <property type="entry name" value="PH_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05581">
        <property type="entry name" value="STKc_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000191">
        <property type="entry name" value="3-phosphoinositide-dependent protein kinase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000405">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000587">
        <property type="entry name" value="Phosphoinositide-dependent kinase 1, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000324">
        <property type="entry name" value="Phosphoinositide-dependent kinase 1, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033931">
        <property type="entry name" value="PDK1-typ_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039046">
        <property type="entry name" value="PDPK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF163">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14593">
        <property type="entry name" value="PH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="248"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="217"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="406"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="767"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="809"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="406"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="418"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="447"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="770"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="816"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="838"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF98400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="853" mass="94679" checksum="CE16AA5A55F2D404" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSA3</accession>
    <name>A0AAU9FSA3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cleavage and polyadenylation specificity factor subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_06934</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM CPSF6/7 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FSA3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12643">
        <property type="entry name" value="RRM_CFIm68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034772">
        <property type="entry name" value="CPSF6/7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057951">
        <property type="entry name" value="CPSF6/7_RSLD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034769">
        <property type="entry name" value="CPSF6_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23204">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25524">
        <property type="entry name" value="RSLD_CPSF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="93"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="16"/>
            <end position="83"/>
        </location>
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</entry>
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        <name type="scientific">Drosophila madeirensis</name>
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                <person name="Tomihara K."/>
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                <person name="Yamamoto D."/>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
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        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
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        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
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        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
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        <property type="entry name" value="RCC1-like_dom"/>
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        <property type="entry name" value="Ub-ligase/GTPase-reg"/>
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        <property type="entry name" value="HECT AND RLD DOMAIN CONTAINING E3 UBIQUITIN LIGASE 4, ISOFORM C"/>
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    </dbReference>
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        <property type="entry name" value="WD40_RLD"/>
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    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="RCC1_3"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
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        <location>
            <begin position="55"/>
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        </location>
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        </location>
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        <location>
            <begin position="158"/>
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        </location>
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        <location>
            <begin position="213"/>
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        </location>
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    <feature type="repeat" description="RCC1" evidence="7">
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        <location>
            <begin position="729"/>
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        </location>
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        <location>
            <position position="1023"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF98602.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1055" mass="118611" checksum="7060A70E433039F4" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSE0</accession>
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        <name evidence="3" type="ORF">DMAD_06645</name>
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        <name type="scientific">Drosophila madeirensis</name>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        </source>
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    </dbReference>
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    </dbReference>
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            <begin position="7"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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            <end position="299"/>
        </location>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF98485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="299" mass="33502" checksum="9BF67CA36457BD6E" modified="2024-11-27" version="1">MSTLQLEFSQAMADFKKMFPDIDREVIEAILRANLGAVDQTIDALLAMSIDNQSLINLNDSDKDLRRNVQLVDTTDGIGDSGAAAAAASAGALLTATASASPNHHNTGASPKMRPLGTSNRINSQNNTPTKRSLSSSSSTARRWNPPILGVLPPGFLRIAPAAGQQDFELPDEQFALMLQNEEFMNQLRWNQDFMNALEKEQTGKTKGEDDAPFQERLKNMGKMSRKKFLQMARVFTWQRNKKVTTAKQIDSLPLREEPSDDEDHHHHHQQQQQQQQQQSSQSQSQQQQQQQGQLQLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSE4</accession>
    <name>A0AAU9FSE4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">DMAD_06835</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FSE4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027854">
        <property type="entry name" value="STMP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15054">
        <property type="entry name" value="DUF4535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF98768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="99" mass="11310" checksum="3CB77E76B4325619" modified="2024-11-27" version="1">MKLLGLGLQLVKARSGAGTSRPSYSCCPEMQDALPYCPKKPFWNFKRTLCFSMLTFGAGIYTGIYISQNYEVPRVDDPQKLVQKMNEKLRELIDDNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FSE8</accession>
    <name>A0AAU9FSE8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">HECT-type E3 ubiquitin transferase NEDD4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_06775</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FSE8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019871">
        <property type="term" value="F:sodium channel inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008586">
        <property type="term" value="P:imaginal disc-derived wing vein morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045879">
        <property type="term" value="P:negative regulation of smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048260">
        <property type="term" value="P:positive regulation of receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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</entry>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
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                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="8 13" id="KW-0675">Receptor</keyword>
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        <location>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    <sequence length="355" mass="40218" checksum="A96D5EFF74343AA9" modified="2024-11-27" version="1">MLLHSKLFTDASSRSIGALNRINFNTRFVLKTLMTICPGTVLLVFMVSLWIIASWTLRQCERFHDEEHANLLNSMWLTAITFLCVGYGDIVPNTYCGRGITLTCGMVGAGCTALLVAVVSRKLELTRAEKHVHNFMMDTQLTKRLKNAAANVLRETWLIYKHTRLVKRVNPGRVRTHQRKFLLAIYALRKVKMDQRKLMDNANTITDMAKTQNTVYEIISDMSSRQDAIEERLTNLEDKMQNLQEHMESLPDLLSRCLTQHQERIEQRRNFLHPDTAAAPIQQPTPQSMFNAAPMLFPHSRSVPSSNNAAATYHWPTSPILPPISSRTPHLVPDTHMPSNGSAVNSYASSNKYGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        <name evidence="15" type="ORF">DMAD_02593</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Insecta</taxon>
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            <taxon>Diptera</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15 16">RF1</strain>
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        <property type="project" value="UniProtKB-UniRule"/>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Kinesin family member 13A"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform E"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
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    </dbReference>
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        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
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    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
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</entry>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </source>
    </evidence>
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</entry>
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                <person name="Yamamoto D."/>
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    </dbReference>
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        </source>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4B8</accession>
    <name>A0AAU9G4B8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MAP kinase-activating death domain protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG02547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1011" mass="109298" checksum="1CB3978ABC21E8C7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4D5</accession>
    <name>A0AAU9G4D5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polyadenylate-binding protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_02191</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G4D5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061574">
        <property type="term" value="C:ASAP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481:SF0">
        <property type="entry name" value="LD23870P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481">
        <property type="entry name" value="RIBONUCLEIC ACID BINDING PROTEIN S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="163"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="242"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="412"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="282"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="328"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFG02778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="450" mass="50530" checksum="5D0E991C51BD4B8D" modified="2024-11-27" version="1">MSKRYSRRILSSDEDEDQTQDTKPQKCEHVGFVDSENVHLHACRRKTCSTKNFEANSGTLRSVETQTGRESDSSTTKIKQEQVENIDNELDIPKTNAAGCQWPKLNKYPEVKKEAPEATRAVDPLRIVNQMGFHFHWATLDRQRCRLPCVHTANVSSVPLESVKIYIGNLSPLVKQEHIGQIFGHFGTIRTVDFPSDRHPINPQGRGFAFVHYVCPSDCARAIKHMNGCKFGDTFIVVSPLRKKPSRDRHSRYAPRSPRSPRAHRSNRDDSRRQRFPSSHHSSSRNRRERSRSPMRRRGVSPSSMSALQSRHRAAYSPITPPKRSRRTNPVTTGGPSSTHLPVMGLGEPNPQNLQPLLVPKLEITDNAAMYPQSPGTATRQNLQPLLAPSTSVLWRPPGPVYSPITSPNLPVMGVGEPNPQNLQPLLVPPATPQNNKNRSDNGERFSTPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4D6</accession>
    <name>A0AAU9G4D6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Neuropathy target esterase sws</fullName>
            <ecNumber evidence="4">3.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Swiss cheese</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_01917</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Its specific chemical modification by certain organophosphorus (OP) compounds leads to distal axonopathy. Plays a role in the signaling mechanism between neurons and glia that regulates glia wrapping during development of the adult brain. Essential for membrane lipid homeostasis and cell survival in both neurons and glia of the adult brain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with Pka-C3; interaction inhibits the catalytic function of Pka-C3 and the esterase activity of sws.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NTE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="4"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046470">
        <property type="term" value="P:phosphatidylcholine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd07225">
        <property type="entry name" value="Pat_PNPLA6_PNPLA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000010">
        <property type="entry name" value="neuropathy target esterase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000001">
        <property type="entry name" value="neuropathy target esterase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000122">
        <property type="entry name" value="Neuropathy target esterase sws"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000135">
        <property type="entry name" value="Neuropathy target esterase sws"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001423">
        <property type="entry name" value="LysoPLipase_patatin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056556">
        <property type="entry name" value="NTE1_P-loop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF29">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE SWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24179">
        <property type="entry name" value="NTE_Ploop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01237">
        <property type="entry name" value="UPF0028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 19" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="14 19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="22">
        <location>
            <begin position="175"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="22">
        <location>
            <begin position="506"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="22">
        <location>
            <begin position="585"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="23">
        <location>
            <begin position="939"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1321"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="19">
        <location>
            <begin position="943"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="19">
        <location>
            <begin position="970"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1345"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1410"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="19">
        <location>
            <position position="972"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="1092"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG02409.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1443" mass="163095" checksum="805D935160C1EC29" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4E7</accession>
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        <submittedName>
            <fullName evidence="19">Potassium voltage-gated channel protein eag</fullName>
        </submittedName>
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        <name evidence="19" type="ORF">DMAD_02225</name>
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    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
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    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.260:FF:000003">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000009">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000009">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003949">
        <property type="entry name" value="K_chnl_volt-dep_EAG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01464">
        <property type="entry name" value="EAGCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="367"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="17">
        <location>
            <begin position="43"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="18">
        <location>
            <begin position="113"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="16">
        <location>
            <begin position="594"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="737"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="786"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="836"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="879"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="979"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1076"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1145"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1229"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="758"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="791"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="836"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="1076"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1089"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1157"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1172"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1240"/>
            <end position="1258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFG02818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1284" mass="137122" checksum="F68CFABD55DCE2CD" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4F5</accession>
    <name>A0AAU9G4F5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Neuropathy target esterase sws</fullName>
            <ecNumber evidence="4">3.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Swiss cheese</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_01917</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Its specific chemical modification by certain organophosphorus (OP) compounds leads to distal axonopathy. Plays a role in the signaling mechanism between neurons and glia that regulates glia wrapping during development of the adult brain. Essential for membrane lipid homeostasis and cell survival in both neurons and glia of the adult brain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with Pka-C3; interaction inhibits the catalytic function of Pka-C3 and the esterase activity of sws.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NTE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="4"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046470">
        <property type="term" value="P:phosphatidylcholine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000010">
        <property type="entry name" value="neuropathy target esterase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000122">
        <property type="entry name" value="Neuropathy target esterase sws"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000135">
        <property type="entry name" value="Neuropathy target esterase sws"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000015">
        <property type="entry name" value="Patatin like phospholipase domain containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001423">
        <property type="entry name" value="LysoPLipase_patatin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056556">
        <property type="entry name" value="NTE1_P-loop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF29">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE SWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24179">
        <property type="entry name" value="NTE_Ploop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01237">
        <property type="entry name" value="UPF0028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 19" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="14 19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="22">
        <location>
            <begin position="175"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="22">
        <location>
            <begin position="506"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="22">
        <location>
            <begin position="585"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="23">
        <location>
            <begin position="939"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1282"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="19">
        <location>
            <begin position="943"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="19">
        <location>
            <begin position="970"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1306"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1371"/>
            <end position="1404"/>
        </location>
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    <feature type="active site" description="Nucleophile" evidence="19">
        <location>
            <position position="972"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="1082"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00006636"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011476"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00019369"/>
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            <dbReference type="ARBA" id="ARBA00022473"/>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="ARBA" id="ARBA00022801"/>
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            <dbReference type="ARBA" id="ARBA00022824"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025020"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030862"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049531"/>
        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
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    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG02411.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1404" mass="158666" checksum="9E84332AF59572EA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEA0</accession>
    <name>A0AAU9GEA0_DROMD</name>
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        <recommendedName>
            <fullName evidence="4">Wurstfest</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">DMAD_04779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
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        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG06225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="44495" checksum="5183B2CAF3C58D53" modified="2024-11-27" version="1">MRIGMTGGQLSQRVSNLSLSSGGCRMEQLGSGSGSSSSLTGSASVGTNTIGNGLVNDPAFAYQSGSQSARQYVHLPGTHLPKQPQYSAGNLQGMEAAGNLSRSGKALTARYHTPSSYGFEGEQMYGQAKGQLAPPMPPPPYALGRWSSTRLFEFENNTPSPCPGSGPMAMSNGTIQLRLRDGVRIDMTLDKAVRVLNQRSMVAVALSRNTSNSAMIHPNGRILQSGSKVEIVTYDGMKGNNFVRYAKMWYKGVSFTSESCALIYMVDTAGTRTTTDTFTDLTKDYTLAVFYDDSRHGPSYVAEAHEVIANASYSCAEDGTEIYDINGFRITQAADGLVKVTRVYNKCLIRTSPSNGSATLTTPGIHCTASLGKTSHLFVRRNEKRMHFDGSCFIVRNAGHSAGFNENNLLIVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEA1</accession>
    <name>A0AAU9GEA1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Protein enabled</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_05037</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ena/VASP family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GEA1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01207">
        <property type="entry name" value="EVH1_Ena_VASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000011">
        <property type="entry name" value="protein enabled isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000047">
        <property type="entry name" value="vasodilator-stimulated phosphoprotein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038023">
        <property type="entry name" value="VASP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014885">
        <property type="entry name" value="VASP_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202:SF22">
        <property type="entry name" value="PROTEIN ENABLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
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            <begin position="309"/>
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            <begin position="313"/>
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        </location>
    </feature>
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            <begin position="378"/>
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        </location>
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        <location>
            <begin position="472"/>
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        </location>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
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        <source>
            <dbReference type="EMBL" id="BFG06554.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="821" mass="87862" checksum="32BEB81B7FA89204" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEB1</accession>
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        <recommendedName>
            <fullName evidence="9">Protein kinase C-like 3</fullName>
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        <name evidence="7" type="ORF">DMAD_05038</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG06569.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="934" mass="101173" checksum="B8EB68752ECBAD3D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEB2</accession>
    <name>A0AAU9GEB2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Regulatory protein zeste</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_04790</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in transvection phenomena (= synapsis-dependent gene expression), where the synaptic pairing of chromosomes carrying genes with which zeste interacts influences the expression of these genes. Zeste binds to DNA and stimulates transcription from a nearby promoter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Self-associates forming complexes of several hundred monomers.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="APONTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21411:SF0">
        <property type="entry name" value="REGULATORY PROTEIN ZESTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13873">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="8">
        <location>
            <begin position="55"/>
            <end position="131"/>
        </location>
    </feature>
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        <location>
            <begin position="341"/>
            <end position="392"/>
        </location>
    </feature>
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        <location>
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            <end position="362"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="Pfam" id="PF13873"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG06240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="493" mass="55287" checksum="A5B793F3065EB0FA" modified="2024-11-27" version="1">MVNKQYTANDLEAFMKIAANWQNSNHSLLEGVDLKTEMTQYMNNPSMNMYKKRERTQNWNHQEKKYLLDLCRKDMRIIENKRLDAGLTAVKNKAWKIIHQKFSNQFGTDRTCNRLKEQWRRMKACTRNEILDYNNRLARFGPEVADRKKPSPFTFEVWDFMQEAKKACKSEALDGIDYSKIPLALEEGFDYREDYKFNGDEHDMDDSRTPPQEMCDVDIKEEEHNETFDEKYNSHHLNQSDILGGAERLSVSPNHSRNGIHEAESPATVSTAVALDASAGGGGFMPGAADMSGFQANFNMNNISATLEALNALRTGQFPSAAAAAAAAIQQHQAQAQAQAQAQVVALQQHKSSSNNNNNNNNNEDDDELLKPSPKRRRTNSGSVLGIEDQPQALTTTAMPLATDCQALERSSSSSNGLGVGVATVSPSSSGGSNSNNFELRLFMEMQSKEHMMRMKILEVQLQSAKHSRDLIEINKTLALQKLQELASKRLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEC4</accession>
    <name>A0AAU9GEC4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitogen-activated protein kinase kinase kinase kinase</fullName>
            <ecNumber evidence="9">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_05039</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Serine/threonine kinase that plays a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="9"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GEC4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008349">
        <property type="term" value="F:MAP kinase kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06613">
        <property type="entry name" value="STKc_MAP4K3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000031">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021160">
        <property type="entry name" value="MAPKKKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF18">
        <property type="entry name" value="HAPPYHOUR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038172">
        <property type="entry name" value="MAPKKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="26"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="15">
        <location>
            <begin position="854"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="417"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="592"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="728"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="778"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="426"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="448"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="592"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="604"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="728"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="780"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="817"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="32"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038172-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038172-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG06584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1213" mass="131370" checksum="BA141437B9C298ED" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEC5</accession>
    <name>A0AAU9GEC5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3',5'-cyclic-AMP phosphodiesterase</fullName>
            <ecNumber evidence="4">3.1.4.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_04799</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.53" evidence="4"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GEC5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000096">
        <property type="entry name" value="High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000002">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000555">
        <property type="entry name" value="Phosphodiesterase 8, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057304">
        <property type="entry name" value="PDE8-like_REC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23198">
        <property type="entry name" value="PDE8A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0114">cAMP</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="12">
        <location>
            <begin position="332"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="PDEase" evidence="13">
        <location>
            <begin position="569"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="128"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="651"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="651"/>
            <end position="655"/>
        </location>
        <ligand>
            <name>AMP</name>
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        </source>
    </evidence>
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</entry>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </location>
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        </source>
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        <source>
            <dbReference type="Pfam" id="PF13873"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG06614.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
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        </location>
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        </location>
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        <location>
            <begin position="386"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="500"/>
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        </location>
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        <location>
            <begin position="13"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG06141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="554" mass="59775" checksum="E8AC7F8262A5C920" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GEG7</accession>
    <name>A0AAU9GEG7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase C-like 3</fullName>
        </recommendedName>
    </protein>
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        <name evidence="7" type="ORF">DMAD_05038</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
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    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08678">
        <property type="entry name" value="C2_C21orf25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
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        <property type="entry name" value="C2_dom"/>
    </dbReference>
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        <property type="entry name" value="C2CD2/C2CD2L"/>
    </dbReference>
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        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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            <begin position="758"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="862"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="70"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="139"/>
            <end position="170"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="372"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1039"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1203"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1153"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="146"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="373"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="387"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="494"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="504"/>
            <end position="515"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="518"/>
            <end position="528"/>
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            <begin position="1114"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG06403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1225" mass="134152" checksum="ACFE60B984DB4A18" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EWR5</accession>
    <name>A0AAU9EWR5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_09618</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
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        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="11">Belongs to the protein-tyrosine phosphatase family. Receptor class subfamily.</text>
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    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome O"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
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    <dbReference type="CDD" id="cd14550">
        <property type="entry name" value="R5-PTP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14549">
        <property type="entry name" value="R5-PTPc-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001386">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase gamma"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:002711">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase gamma"/>
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        <property type="entry name" value="receptor-type tyrosine-protein phosphatase zeta"/>
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        <property type="entry name" value="Tyrosine-protein phosphatase 99A"/>
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        <property type="entry name" value="Tyrosine-protein phosphatase 99A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Uncharacterized protein, isoform D"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
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    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="FN3_sf"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
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        <property type="entry name" value="PTP_cat"/>
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        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
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        <property type="entry name" value="Tyr_Pase_AS"/>
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        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
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        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF6">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="fn3"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="556"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="136"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="238"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="338"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="435"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="13">
        <location>
            <begin position="641"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="14">
        <location>
            <begin position="823"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="13">
        <location>
            <begin position="929"/>
            <end position="1181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF91308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1226" mass="139518" checksum="0F0320F40213B180" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EWR7</accession>
    <name>A0AAU9EWR7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Complexin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_07765</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Positively regulates a late step in synaptic vesicle exocytosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complexin/synaphin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EWR7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:regulation of neurotransmitter secretion"/>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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        </source>
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        <source>
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        </source>
    </evidence>
    <sequence length="1540" mass="164190" checksum="B83B0703389D9113" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EWT4</accession>
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        <submittedName>
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        <name evidence="8" type="ORF">DMAD_09359</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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            <strain evidence="8 9">RF1</strain>
        </source>
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        </subcellularLocation>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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        <recommendedName>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
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    </dbReference>
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        <property type="entry name" value="PROTEIN WINGED EYE"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3_TNRC18"/>
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    </dbReference>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EWV0</accession>
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        <submittedName>
            <fullName evidence="12">Neprilysin-4</fullName>
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        <name evidence="12" type="ORF">DMAD_09740</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
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        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
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        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
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        <text evidence="3">Belongs to the peptidase M13 family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="6">Oxysterol-binding protein</fullName>
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        <name evidence="10" type="ORF">DMAD_09430</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EX47"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13286">
        <property type="entry name" value="PH_OPR5_ORP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2720:FF:000002">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000030">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000004">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF102">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="791"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="63"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="226"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="681"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="246"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="713"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF91060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="809" mass="90909" checksum="3DA4DCCA2F0788CC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EX54</accession>
    <name>A0AAU9EX54_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CLIP domain-containing serine protease 14D-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_09808</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EX54"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253:SF153">
        <property type="entry name" value="SERINE PROTEASE HEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="2"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF91558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="153" mass="17262" checksum="4452918D0C610FBD" modified="2024-11-27" version="1">MFKIEKIIVHPDYKNNTLGDDLAVVRLDREVPFSAFVQPMCMPHSWEGPANFVAAYVDLIGYEMGNKKRSRLIKAYEHISDPQSCLLRYPEISQRQMCGYIKGAELQPGSPLIGVRVYKGLPANYYLIGNLVGGYDPSLSAPYLFISIAPYRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EX55</accession>
    <name>A0AAU9EX55_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL9m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L9, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_09301</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EX55"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.5.10:FF:000005">
        <property type="entry name" value="39S ribosomal protein L9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="entry name" value="Ribosomal protein L9, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_bL9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_bL9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_bL9_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Ribosomal protein L9" evidence="9">
        <location>
            <begin position="22"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF90857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="202" mass="23262" checksum="B58ED456B9CA3058" modified="2024-11-27" version="1">MRPKHFIYELVEDTQVKKRPNMEVVLKTFVEGVGDKGDVVSMKPHFVYNKLLLPGLAAYKTPENIAKYAKTDAEKSAVKHSSAYAQRTVNMLETIVLAVVMNKDEPWVLEPWHIKASLRKAGFHCKEECITLPKERIEGPDLKKESKDFQCIITINKLEQATLKCRLHHWSTDPSERLPYVLEHWKMQSEPLLDVGSPEKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EX57</accession>
    <name>A0AAU9EX57_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hermansky-Pudlak syndrome 5 protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_09818</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Has a role in the biogenesis of eye pigment granules. Eye pigment granules are specialized forms of late endosomes or lysosomes. Biogenesis of pigment granules in the eye requires molecular components required for protein delivery to lysosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the HPS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EX57"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031409">
        <property type="term" value="F:pigment binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048066">
        <property type="term" value="P:developmental pigmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000968">
        <property type="entry name" value="Hermansky-Pudlak syndrome 5 protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056499">
        <property type="entry name" value="Beta-prop_HPS5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035431">
        <property type="entry name" value="HPS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056446">
        <property type="entry name" value="TPR_HPS5_insects"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23287:SF18">
        <property type="entry name" value="BLOC-2 COMPLEX MEMBER HPS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23287">
        <property type="entry name" value="RUBY-EYE2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23756">
        <property type="entry name" value="Beta-prop_HPS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23757">
        <property type="entry name" value="TPR_HPS5_insect"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037475">
        <property type="entry name" value="BLOC-2_complex_Hps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0608">Pigment</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="5 7" id="KW-0844">Vision</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="HPS5-like beta-propeller" evidence="9">
        <location>
            <begin position="18"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="HPS5 TPR" evidence="10">
        <location>
            <begin position="585"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="426"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="426"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF91571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="831" mass="94296" checksum="9A14C992D7F72BFC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EX68</accession>
    <name>A0AAU9EX68_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Wiskott-Aldrich syndrome protein family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_09448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EX68"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000040">
        <property type="entry name" value="GUK-holder, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF91090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="23510" checksum="6E4E82C49961F428" modified="2024-11-27" version="1">MPFVQRVVQPVNVAQATAASLGHASTRFHCAPGKCKNNNCINKQSPEAGTDAVDCRATSPTSPSLTRVLVHVNGDGEDGAGQPHPPAALPMKKLKQHVEFLSTSPTHGPGRASQSLPNSRHPSQQRPAMARNASAPHSPTAGSSKVVSGHEFDSISNITLSNALRQLASLVLIASDIFDDLQRDLQSVGERAGRVQRKIAAVERRVCAYDPKMVTVRKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EX69</accession>
    <name>A0AAU9EX69_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Sodium/calcium exchanger 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_09314</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Ca(2+)(in) + 3 Na(+)(out) = Ca(2+)(out) + 3 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:69955"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC8 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EX69"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005432">
        <property type="term" value="F:calcium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2030:FF:000024">
        <property type="entry name" value="sodium/calcium exchanger 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051171">
        <property type="entry name" value="CaCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038081">
        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003644">
        <property type="entry name" value="Calx_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004836">
        <property type="entry name" value="Na_Ca_Ex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032452">
        <property type="entry name" value="Na_Ca_Ex_C-exten"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00845">
        <property type="entry name" value="caca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878:SF65">
        <property type="entry name" value="NA_CA-EXCHANGE PROTEIN, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878">
        <property type="entry name" value="SODIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03160">
        <property type="entry name" value="Calx-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16494">
        <property type="entry name" value="Na_Ca_ex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01259">
        <property type="entry name" value="NACAEXCHNGR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00237">
        <property type="entry name" value="Calx_beta"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141072">
        <property type="entry name" value="CalX-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 21" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043616925" evidence="21">
        <location>
            <begin position="27"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="787"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="820"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="859"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="890"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="930"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="22">
        <location>
            <begin position="426"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="22">
        <location>
            <begin position="543"/>
            <end position="642"/>
        </location>
    </feature>
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        </source>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00033667"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00237"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF90872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="956" mass="107202" checksum="1222AAE3CE231798" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0C6</accession>
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        <submittedName>
            <fullName evidence="8">Modifier of mdg4-like</fullName>
        </submittedName>
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        <name evidence="8" type="ORF">DMAD_08947</name>
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    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="protein sequence ID" value="BFF90419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mod(Mdg4), isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR007588">
        <property type="entry name" value="Znf_FLYWCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
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    </dbReference>
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        <property type="entry name" value="MODIFIER OF MDG4"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="BFF90419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="566" mass="62518" checksum="FE68F47C8A83A9FD" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0E5</accession>
    <name>A0AAU9F0E5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized abhydrolase domain-containing protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">DMAD_07821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F0E5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="43"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="310"/>
        </location>
    </feature>
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        <location>
            <begin position="362"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
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        <location>
            <begin position="67"/>
            <end position="106"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="169"/>
        </location>
    </feature>
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        <location>
            <begin position="210"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="362"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF88954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="392" mass="40793" checksum="7156731CB3EC1A9B" modified="2024-11-27" version="1">MELSEREARVRRREEKVRTQCLWRNLAIKKRWVEAREKALVEREAQVRKKEQQPGIPSPKRTRDHSPTTMPTHVHTPTHVHTPTTTPTHAHTPTTAPTHPTEPTPTSQAEVSWEERLRQAEEEEEAMWGAEEPTAGAREAAQEVPAAPEEERQQHQQQPGPGWQGPQGAVIGPFVSQEVRTAVRNGMVWHHQTLHITWASGPAPVDPGPEEARVWEEAPSHSKAETGGPNNNGREHGGRQRSGRGTKTVAEGGGAGGSGASAARRGEAGGSGARCAGGTEGVASGCGDPASRGPGTGGEGRAGGAATIGGATGGERGRVLGAMGAGSVAVARAGTTGANAQAADSRDDTTGPTVQATIVMPRTATRTPADAPATDVGANGAPMDAARARGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0E9</accession>
    <name>A0AAU9F0E9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Sodium/potassium-transporting ATPase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_09316</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Cell membrane</location>
            <topology evidence="1 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F0E9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090533">
        <property type="term" value="C:cation-transporting ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098797">
        <property type="term" value="C:plasma membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02608">
        <property type="entry name" value="P-type_ATPase_Na-K_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000038">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000003">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000001">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000004">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000095">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005775">
        <property type="entry name" value="P-type_ATPase_IIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01106">
        <property type="entry name" value="ATPase-IIC_X-K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF13">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00121">
        <property type="entry name" value="NAKATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 20" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 20" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7" id="KW-0740">Sodium/potassium transport</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="867"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="966"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="999"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="22">
        <location>
            <begin position="56"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00037422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00038795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BFF90879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1037" mass="115068" checksum="63AE624101396E25" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F8R5</accession>
    <name>A0AAU9F8R5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Aminopeptidase N</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_10207</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F8R5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000013">
        <property type="entry name" value="Aminopeptidase N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000008">
        <property type="entry name" value="aminopeptidase N isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF294">
        <property type="entry name" value="THYROTROPIN-RELEASING HORMONE-DEGRADING ECTOENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 19" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
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    <keyword evidence="8 12" id="KW-0862">Zinc</keyword>
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        <location>
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            <end position="58"/>
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        <location>
            <begin position="160"/>
            <end position="361"/>
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            <begin position="399"/>
            <end position="627"/>
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            <begin position="712"/>
            <end position="1019"/>
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        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
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    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="475"/>
        </location>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="478"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
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    <feature type="site" description="Transition state stabilizer" evidence="13">
        <location>
            <position position="560"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
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        <source>
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        </source>
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        <source>
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        </source>
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        <source>
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        <source>
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        </source>
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        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
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            <dbReference type="EMBL" id="BFF92070.1"/>
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            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1060" mass="120754" checksum="C1D0C6E216CA2E52" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="466" mass="51226" checksum="803E597B241D5626" modified="2024-11-27" version="1">MANPMRIKEETSGEDPSSTETIEIPDREDDDICELEHLRKLFIGGLAPYTTEEGLKVFYGQWGKVVDVVVMRDAATKRSRGFGFITYTKSTMVDKAQENRPHIIDGKTVEAKRALPRPERETRETNISVKKLFVGGLKDNHDEDCLREYFVQFGRVVSVKLLTDKTTGKRRGFAFIEFDDYDAVDKAILQKQHSIKYVHVDVKKSIYNLEKKDKQSQGPPTNGSKPPQQQPQQPPQPHNGNMQAPGYRPPVPPQQPMPPYPHQPPPPINAPPPNYNYWGPPPPMQGYYQQPPPQMNGWNGYPQNGWNAPPPPPPGPQQWHPSQWGCPPPVQPPAGAGGPPMDHHRNGPPPPAPGNWNMPPNAPPPVPGAPLPQGPPPHQQPPPNFGTGYQQNYGGGPTKHNNMNANRMNPYAAAPPNNYHNAQPPASGYGPYSSPAPPPNGNAPLPKGVSNGSVAVGGSANSKYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F8S6</accession>
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        <name evidence="11" type="ORF">DMAD_10218</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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            <strain evidence="11 12">RF1</strain>
        </source>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071:SF17">
        <property type="entry name" value="CATION-INDEPENDENT MANNOSE-6-PHOSPHATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00878">
        <property type="entry name" value="CIMR"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="820"/>
            <end position="841"/>
        </location>
    </feature>
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            <begin position="52"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="10">
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        </location>
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    <feature type="domain" description="MRH" evidence="10">
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        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="10">
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            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="790"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
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            <begin position="796"/>
            <end position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005578">
        <property type="entry name" value="Yif1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083">
        <property type="entry name" value="YIP1 INTERACTING FACTOR HOMOLOG YIF1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083:SF0">
        <property type="entry name" value="YIP1D-INTERACTING FACTOR 1, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03878">
        <property type="entry name" value="YIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="311"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="72"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF92099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="406" mass="45235" checksum="5790B102A24FD957" modified="2024-11-27" version="1">MNYNPNAAQGGGRPRPNKRISDMNAMGPSAPMMGGPTFMPPTAGPGMLDPNMYGGAPAAAAAPSTGYGFNVAPSQEPQQQQAPQNYQQPPQQQQQQQQQQQQSFGYAPSQQGGAPPPYGMPAAQAGPGGQFPQFAMLQQPIVQDMAMEYGQRLADQGRQMVENQFERWVPVAKLRYYFAVDNAYVGRKLRLLFFPYIHKDWSLKYDQEHPVQPRYDVNAPDLYLPTMGYITYVIVAGLLLGMQKRFSPEQLGIQASSAMAYSIFELVIYSIALYVMNIKTSLKTLDLLAFTGYKFVNIVACLMFSTLFYRSGYFIALAYTSFSFGFFLLRTLRTKLLQDSAPAVSSNAINYDPYSNPQELDYSGGRKRKLYFLFMVVAGQAVLAFLLSKHLYLPESEVLLSVPKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F8U4</accession>
    <name>A0AAU9F8U4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Protein YIF1B-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_10229</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YIF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F8U4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005578">
        <property type="entry name" value="Yif1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083">
        <property type="entry name" value="YIP1 INTERACTING FACTOR HOMOLOG YIF1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083:SF0">
        <property type="entry name" value="YIP1D-INTERACTING FACTOR 1, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03878">
        <property type="entry name" value="YIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="68"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="81"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF92097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="415" mass="46423" checksum="33FEF32A8C9C4230" modified="2024-11-27" version="1">MNYNPNAGMRNRKWENSQGGGRPRPNKRISDMNAMGPSAPMMGGPTFMPPTAGPGMLDPNMYGGAPAAAAAPSTGYGFNVAPSQEPQQQQAPQNYQQPPQQQQQQQQQQQQSFGYAPSQQGGAPPPYGMPAAQAGPGGQFPQFAMLQQPIVQDMAMEYGQRLADQGRQMVENQFERWVPVAKLRYYFAVDNAYVGRKLRLLFFPYIHKDWSLKYDQEHPVQPRYDVNAPDLYLPTMGYITYVIVAGLLLGMQKRFSPEQLGIQASSAMAYSIFELVIYSIALYVMNIKTSLKTLDLLAFTGYKFVNIVACLMFSTLFYRSGYFIALAYTSFSFGFFLLRTLRTKLLQDSAPAVSSNAINYDPYSNPQELDYSGGRKRKLYFLFMVVAGQAVLAFLLSKHLYLPESEVLLSVPKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F8U6</accession>
    <name>A0AAU9F8U6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_10234</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F8U6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002321">
        <property type="entry name" value="GG12177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21261">
        <property type="entry name" value="BEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21261:SF3">
        <property type="entry name" value="BEATEN PATH VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043695237" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="35"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF92105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="329" mass="38301" checksum="4B24AD14A9932799" modified="2024-11-27" version="1">MHRFWIIVLLLGLLHTKQINGQSDVEVQLVVPRYVERGSSATFKCRHNVQPEILFKVTWLKVDKGKFFEFINGRNPPFRNSTIEGAEIDWDNSNEQQVTLKDVQFDLSGQFYCEVSTDTPIFTKASADELMSVFLPQTGPPTIKFRKRTPFAVGEKLFALCNTTRGRPAPHITWLINGKKVEEKYVRTHHVFSFNGKHQRRTQQQQQTQLSQQQLQQQYYQNQYYNQYHQQYHIPVGQFMDRYDKSLRWPAGARADEFPHFAAHHHEGHHHGGGGGGGAGGSIYNNPFGSLANYHDVGEFHEIHQRNYDINKKKLELKKQQQMELKKHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F8U8</accession>
    <name>A0AAU9F8U8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Protein YIF1B-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_10229</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YIF1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF92098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome O"/>
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        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Yif1_fam"/>
    </dbReference>
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        <property type="entry name" value="YIP1 INTERACTING FACTOR HOMOLOG YIF1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083:SF0">
        <property type="entry name" value="YIP1D-INTERACTING FACTOR 1, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
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    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="257"/>
            <end position="279"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="374"/>
            <end position="396"/>
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            <begin position="1"/>
            <end position="49"/>
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            <begin position="63"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="76"/>
            <end position="117"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004477"/>
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    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="EMBL" id="BFF92098.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="410" mass="45720" checksum="E0316D414325550C" modified="2024-11-27" version="1">MNYNPNAGMRNPQGGGRPRPNKRISDMNAMGPSAPMMGGPTFMPPTAGPGMLDPNMYGGAPAAAAAPSTGYGFNVAPSQEPQQQQAPQNYQQPPQQQQQQQQQQQQSFGYAPSQQGGAPPPYGMPAAQAGPGGQFPQFAMLQQPIVQDMAMEYGQRLADQGRQMVENQFERWVPVAKLRYYFAVDNAYVGRKLRLLFFPYIHKDWSLKYDQEHPVQPRYDVNAPDLYLPTMGYITYVIVAGLLLGMQKRFSPEQLGIQASSAMAYSIFELVIYSIALYVMNIKTSLKTLDLLAFTGYKFVNIVACLMFSTLFYRSGYFIALAYTSFSFGFFLLRTLRTKLLQDSAPAVSSNAINYDPYSNPQELDYSGGRKRKLYFLFMVVAGQAVLAFLLSKHLYLPESEVLLSVPKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F937</accession>
    <name>A0AAU9F937_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PNT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_10313</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
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    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="1037"/>
            <end position="1136"/>
        </location>
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        </location>
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        </location>
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        </location>
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        </location>
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        </location>
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            <begin position="1814"/>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00251"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF92208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1900" mass="210266" checksum="EB382A9F38CF9642" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F938</accession>
    <name>A0AAU9F938_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RUN and FYVE domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_10301</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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</entry>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
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        </source>
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    <sequence length="1875" mass="207586" checksum="CE8D4372BEF50317" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F966</accession>
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        <name evidence="11" type="ORF">DMAD_10326</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11 12">RF1</strain>
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    <keyword evidence="2" id="KW-0677">Repeat</keyword>
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            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="BFF92227.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F968</accession>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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            <strain evidence="7 8">RF1</strain>
        </source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <name evidence="9" type="ORF">DMAD_10689</name>
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            <taxon>Diptera</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FAF5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Interleukin enhancer-binding factor 2"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR049401">
        <property type="entry name" value="DZF_dom_N"/>
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    <dbReference type="InterPro" id="IPR052134">
        <property type="entry name" value="ILF2"/>
    </dbReference>
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        <property type="entry name" value="NT_sf"/>
    </dbReference>
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        <property type="entry name" value="INTERLEUKIN ENHANCER-BINDING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46447:SF1">
        <property type="entry name" value="INTERLEUKIN ENHANCER-BINDING FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20965">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07528">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00572">
        <property type="entry name" value="DZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50152">
        <property type="entry name" value="25A_SYNTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51703">
        <property type="entry name" value="DZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DZF" evidence="8">
        <location>
            <begin position="22"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="364"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF92681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="394" mass="43687" checksum="B7C25BB947234669" modified="2024-11-27" version="1">MVRGSLRGGRPMRGGIRPPFKKTFVPRHPFDLTLAEVFFPKVAPALDDSALTAALLKRNQDLSPTASEQTAIGNLVTKVQAVLDNLVVAPGDFNTCQLEEVRQVGSFKKGTILTGNNVADIVVILKTLPTKEAVEALSKKVEADLKTSMKTEVLTKGDQHTIQTHLRGFDVSTVQAKVRIMIATLPQNLRKLEADIHMDHKLMQNHLAAIRHTRWFEENAHHSSIKVLIRILKDLTKRFEAFSPLSAWMLDLIAHLAIMNNPSRQALPINLAFRRVFQLLSAGLFLPGSAGITDPTEPGHIRVHTAMTLEQQDLCCYTAQTLLRVLTHGGYKHILGMEGNTSIVREMSVWNGVCVSPLTAVYEKPTDKKEGDLEEDIEMIENENEEEGSEDGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAF6</accession>
    <name>A0AAU9FAF6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Malic enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_10631</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FAF6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004473">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05312">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF90">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="10">
        <location>
            <begin position="126"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="9">
        <location>
            <begin position="317"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="501"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF92616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="626" mass="69215" checksum="A921C24671ADCEFF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAF7</accession>
    <name>A0AAU9FAF7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">PHD finger protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_10695</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15552">
        <property type="entry name" value="PHD_PHF3_like"/>
        <property type="match status" value="1"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF92965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="43541" checksum="F4272292CED43472" modified="2024-11-27" version="1">MLHYLAASCLLLALSGLQPATAAIHKRSGAHAANGLGSEESATRSQAPGHSQSKPAPNPSGSVENTDLLDKLSWKCANNASCLYSVANGIMTSYRRGETIKLGLFDLVRLPELDKAATHKHKWGTGRGLASFMDFVSGNAVRVPVGPMVFSVQRSEEDSDYIEVALLKKAASGTGRLQGTGGVGGLLGGGGGGGLLGGGGGGSGIGGGGGLLGGGGGGDSGGGGGLLRRRHQHQDKKQFQMLIPMYLAATTFGWTMVAAKAVGLLTLKALILSKIAFVVAAIVLIKKLMDNASEKMMYQFPEQTPYMMPYGMDYGGLHGGDISPEMYPGSLHHLAMAGGGQLSGHPGHPGLESFGAESHLHSQVSSDGSNHTQVLAALGGLGGLGHKIKREDSWMAKTTPARRPLIYNYVQPHMPYYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FBB1</accession>
    <name>A0AAU9FBB1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Osiris 19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_10926</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FBB1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012464">
        <property type="entry name" value="DUF1676"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21879">
        <property type="entry name" value="FI03362P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21879:SF6">
        <property type="entry name" value="OSIRIS 19, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07898">
        <property type="entry name" value="DUF1676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043728680" description="Osiris 19" evidence="2">
        <location>
            <begin position="21"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF92989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="29056" checksum="90545C095A2EE912" modified="2024-11-27" version="1">MAKLLLVIVGVTALMAAAQAAAGASTQQQLPLQRLIAEEQHKCATGQDSMACIKERAMRFVDNVMSKDSYKLSNLEVRSNGQKASPVHDARARASSSADGFMDALENYIQGHDVSMNLPMADAKITVSARNLANDELSLNLQLNGDEEDGAAVDGEARGKKHRLRKLAMPILVLILLKAITVIPMAIGILKIKAFNALALGFFSFLVSVGLAIFQLCKKIAHDHHHTAHITAHGPWDGRTFGALAAAIPAPVVEQPQQLAVAQDMAYKGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FIU2</accession>
    <name>A0AAU9FIU2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">CD109 antigen</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">TEP1-F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_13024</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Binds covalently through a thioester bond to the pathogen surface resulting in pathogen clearance.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Modulates negatively TGFB1 signaling in keratinocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterodimer of a TEP1-N chain and an TEP1-C chain non-covalently linked. Forms a complex composed of TEP1-N and TEP1-C heterodimer, LRIM1 and APL1C; the interaction stabilizes TEP1-N and TEP1-C heterodimer, prevents its binding to tissues while circulating in the hemolymph and protects the thioester bond from hydrolysis. Mature TEP1 and to a lesser extent full-length TEP1 interact with SPCLIP1; the interaction is induced by microbial infection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterodimer; disulfide-linked. Interacts with TGFB1 and TGFBR1. Forms a heteromeric complex with TGFBR1, TGFBR2 and TGFBR3 in a ligand-independent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000001">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1940:FF:000003">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000155">
        <property type="entry name" value="complement C3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2950:FF:000001">
        <property type="entry name" value="Thioester-containing protein 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.690:FF:000003">
        <property type="entry name" value="Thioester-containing protein 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.20.50.160:FF:000002">
        <property type="entry name" value="Thioester-containing protein 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040839">
        <property type="entry name" value="MG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF136">
        <property type="entry name" value="CD109 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17789">
        <property type="entry name" value="MG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0882">Thioester bond</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043414955" description="CD109 antigen" evidence="20">
        <location>
            <begin position="22"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="21">
        <location>
            <begin position="440"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="22">
        <location>
            <begin position="677"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="23">
        <location>
            <begin position="1289"/>
            <end position="1379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="BFF95674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1401" mass="155178" checksum="7C2F053E69611962" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FIU3</accession>
    <name>A0AAU9FIU3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="13">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_12915</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="13"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FIU3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007435">
        <property type="term" value="P:salivary gland morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05059">
        <property type="entry name" value="PTKc_Tec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09934">
        <property type="entry name" value="SH2_Tec_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11768">
        <property type="entry name" value="SH3_Tec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000052">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000190">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000053">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000045">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 13" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="166"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="15">
        <location>
            <begin position="234"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="350"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="41"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="41"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFF95532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="610" mass="67434" checksum="31387CF13F62CF86" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FIU8</accession>
    <name>A0AAU9FIU8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine-rich adhesin for platelets</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_13018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Slowpoke binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14312:SF1">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14312">
        <property type="entry name" value="CREB/ATF BZIP TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="554"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="669"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="819"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="877"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1013"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1201"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1264"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1292"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1344"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="671"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="819"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="877"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1031"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1059"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1070"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1208"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1219"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1268"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1292"/>
            <end position="1323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF95663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1383" mass="152425" checksum="FB8B037941197B97" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FIV7</accession>
    <name>A0AAU9FIV7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12 15">Uricase</fullName>
            <ecNumber evidence="5 12 15">1.7.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Urate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_13037</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12 15">Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12 15">
            <text>urate + O2 + H2O = 5-hydroxyisourate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:21368"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="EC" id="1.7.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Purine metabolism; urate degradation; (S)-allantoin from urate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12 15">Belongs to the uricase family.</text>
    </comment>
    <dbReference type="EC" id="1.7.3.3" evidence="5 12 15"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FIV7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004846">
        <property type="term" value="F:urate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019628">
        <property type="term" value="P:urate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00445">
        <property type="entry name" value="Uricase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.270.10:FF:000002">
        <property type="entry name" value="Uricase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.270.10">
        <property type="entry name" value="Urate Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002042">
        <property type="entry name" value="Uricase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019842">
        <property type="entry name" value="Uricase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03383">
        <property type="entry name" value="urate_oxi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42874">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42874:SF1">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01014">
        <property type="entry name" value="Uricase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000241">
        <property type="entry name" value="Urate_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00093">
        <property type="entry name" value="URICASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00366">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7 12 15" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>O2</name>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>O2</name>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000241-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000241-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF95689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="349" mass="39505" checksum="EAFC519C42391AD9" modified="2024-11-27" version="1">MFATPLRQPSSASNRTAASASLDGQETPFQYEITDHGYGKDAVKVLHVSRKGPVHTIQEFEVGTHLKLYSKKDYYQGNNSDIVATDSQKNTVYLLAKKFGIESPEKFALLLGQHFLNKYSHVEEAHIHVETYPWQRVCQEETKASTTSVVGQGSSNNFSSIDNRSLHNHAFIFTPTALHYCDVVIRRQDPKQTVITGIKGLRVLKTTQSSFVNFVNDEFRSLPDQYDRIFSTVVDCSWEYSDTDTVNFSRAWQQVKNIILRNFAGDPQVGVSSPSVQHTLYLSEKQVLDVIPQVSVISMTMPNKHYFNFDTKPFQQIVPGDNNEVFIPVDKPHGTIYAQLARKNLNSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FIW0</accession>
    <name>A0AAU9FIW0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_12920</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00192">
        <property type="entry name" value="PTKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000373">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000384">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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</entry>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        </lineage>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15 16">RF1</strain>
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        <text evidence="10">Interacts with EMP65.</text>
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        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
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        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SLP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJ66"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000099">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045120">
        <property type="entry name" value="Suco/Slp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953">
        <property type="entry name" value="MEMBRANE PROTEIN CH1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953:SF0">
        <property type="entry name" value="SUN DOMAIN-CONTAINING OSSIFICATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="822"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="14">
        <location>
            <begin position="177"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="34"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="99"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="156"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="510"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="570"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="850"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="986"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1080"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1143"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1201"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="758"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="111"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="527"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="581"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="986"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1025"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1081"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1099"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1159"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1178"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1201"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1215"/>
            <end position="1230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF95700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1246" mass="135267" checksum="E85DDD336D97689B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJ69</accession>
    <name>A0AAU9FJ69_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">receptor protein serine/threonine kinase</fullName>
            <ecNumber evidence="5">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_13128</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
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    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9FJ69"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14144">
        <property type="entry name" value="STKc_BMPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23596">
        <property type="entry name" value="TFP_LU_ECD_Tkv"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000021">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000055">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000472">
        <property type="entry name" value="Activin_recp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003605">
        <property type="entry name" value="GS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF106">
        <property type="entry name" value="RECEPTOR PROTEIN SERINE_THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01064">
        <property type="entry name" value="Activin_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08515">
        <property type="entry name" value="TGF_beta_GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00467">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51256">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17 22" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="GS" evidence="21">
        <location>
            <begin position="169"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="200"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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</entry>
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    <accession>A0AAU9FJ71</accession>
    <name>A0AAU9FJ71_DROMD</name>
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        <submittedName>
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        <name evidence="5" type="ORF">DMAD_13135</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <source>
            <dbReference type="EMBL" id="BFF95809.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1898" mass="208011" checksum="7AE49C0309EF181A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJ75</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="6">1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase</fullName>
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        </recommendedName>
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    </gene>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 9">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
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        <text evidence="8">Binds 1 Ca(2+) ion per subunit.</text>
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        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF123">
        <property type="entry name" value="PEPTIDASE M14 CARBOXYPEPTIDASE A DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 17" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 15" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043493729" description="Zinc carboxypeptidase A 1" evidence="15">
        <location>
            <begin position="21"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="16">
        <location>
            <begin position="125"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF95667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="426" mass="48088" checksum="B6BA033142486500" modified="2024-11-27" version="1">MRLLWLCVATLALLLVLASAATLKDTPKERYDGYGVYRLSVKNKLQQAVIEQLSQLSSKYNIWKDYDEHSQEVHIMVAPAELQHFQALLSLHAISSEQIIENVQDLIDAEQVTPAADSAPFGWTKYYELSEIEAWLDSILAAYPAVTEEFIVGQSYEGRTIRGIKISHKAGNPGIFIESNIHAREWITSASATWFINELLTSQEPAVRNLAESYDWHIVPVFNVDGFEYSHAKNRMWRKTRQPHATNECIGVDANRNFDSHWLENNGASSNPCSETFAGDVPGSEPEAKALTEYLKTIENEFSVYISFHSYGQYLLSPYGHTKEEFPDNYDDLLEIGEAFATAVESLPYGTAYRYGSTADVLYVATGTSVDWVFNELNKKVAFTIEYRDQGRYGFILPPVQILPNCQELMAGMLALIDKTEELGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJ86</accession>
    <name>A0AAU9FJ86_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Voltage-dependent L-type calcium channel subunit beta-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_13155</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calcium channel beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJ86"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11863">
        <property type="entry name" value="SH3_CACNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003279">
        <property type="entry name" value="Ca2+-channel-protein-beta-subunit, isoform M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000049">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit beta-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046937">
        <property type="entry name" value="CAB1-4_N_A-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000584">
        <property type="entry name" value="VDCC_L_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11824">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12052">
        <property type="entry name" value="VGCC_beta4Aa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01626">
        <property type="entry name" value="LCACHANNELB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="82"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="159"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="431"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="527"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="625"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="533"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="642"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="707"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF95837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="829" mass="94348" checksum="78990D08C50BA320" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJR4</accession>
    <name>A0AAU9FJR4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Deoxyuridine 5'-triphosphate nucleotidohydrolase</fullName>
            <shortName evidence="9">dUTPase</shortName>
            <ecNumber evidence="9">3.6.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">dUTP pyrophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_13181</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and through its action efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis. Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor. Essential for embryonic development.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Involved in nucleotide metabolism via production of dUMP, the immediate precursor of thymidine nucleotides, and decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>dUTP + H2O = dUMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the dUTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="9"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJR4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046081">
        <property type="term" value="P:dUTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.40.10:FF:000004">
        <property type="entry name" value="Deoxyuridine triphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008181">
        <property type="entry name" value="dUTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00576">
        <property type="entry name" value="dut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001862">
        <property type="entry name" value="PRK00601.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241:SF0">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase-like" evidence="11">
        <location>
            <begin position="20"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="136"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF95872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="173" mass="18602" checksum="BFC440DA4DA19800" modified="2024-11-27" version="1">MKIEKCVLRFAKLTEHALEPVRGSAKAAGVDLRSAYDVVVPARGKAIVKTDLQVQVPEGSYGRVAPRSGLAVKNFIDVGAGVVDEDYRGNLGVVLFNHSETDFEVKHGDRIAQFICERIFYPELELVDKLDDTERGEAGFGSTGVKDLPAAKNGNGEKAPPAEPEATPTPIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJR5</accession>
    <name>A0AAU9FJR5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_13289</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJR5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000008">
        <property type="entry name" value="Apoptosis-resistant E3 ubiquitin protein ligase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000013">
        <property type="entry name" value="Apoptosis-resistant E3 ubiquitin protein ligase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000975">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058738">
        <property type="entry name" value="PH-like_AREL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF340">
        <property type="entry name" value="APOPTOSIS-RESISTANT E3 UBIQUITIN PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25916">
        <property type="entry name" value="AREL1_PH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="6">
        <location>
            <begin position="306"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="9">
        <location>
            <begin position="632"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="934"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF96004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="967" mass="109420" checksum="33F9E524CE4FC568" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJR6</accession>
    <name>A0AAU9FJR6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">COP1-interactive protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_13149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJR6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="62"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="394"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF95826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="603" mass="69070" checksum="190C65FF350BB86D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FK30</accession>
    <name>A0AAU9FK30_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable serine/threonine-protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_13305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FK30"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025271">
        <property type="entry name" value="CCDC28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13400">
        <property type="entry name" value="CHEMOKINE C-C MOTIF RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13400:SF4">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 28A-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13270">
        <property type="entry name" value="CCDC28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="146"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="271"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="148"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF96025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="332" mass="36313" checksum="16F86EEC901D4B4D" modified="2024-11-27" version="1">MEPNDEVVERQKLVPNDVETEEEAQSQSAPKSAQTPASPAVPNIKIKGISSGDTTRSRNSASCSIERTISEKDKDKGQQPTATKVTYVNERRPRPQAQGGSGGAGDERFEFKTRPRKLLKDGDALEPTHSSASSLNAITLVSSEWLSPDPTITNTTTAISTSTVTNNNNNNDSTKPNHNDTSNNNNSNTNTPRSQRRKPAAAPATAATSMPNASVHSDKFDDRPIKHHSFVSEVPDVKHMERALLGLLDDFHSGKLKAFGSGCTMDQMTKIREQQESLAKLHFELAAAEEDSLEHGNEFNTNKAQENMLQLMQRLEQLSISIEQLQTSHTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FK32</accession>
    <name>A0AAU9FK32_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ATP-binding cassette sub-family G member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_13376</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FK32"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000891">
        <property type="entry name" value="ATP-binding cassette sub-family G member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF6">
        <property type="entry name" value="PROTEIN WHITE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="476"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="520"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="558"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="587"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="668"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="46"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF96118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="698" mass="78135" checksum="E55FBF3307DA8863" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRF0</accession>
    <name>A0AAU9FRF0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Geranylgeranyl pyrophosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_06358</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRF0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120531">
        <property type="term" value="F:prenyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042811">
        <property type="term" value="P:pheromone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000009">
        <property type="entry name" value="Geranylgeranyl pyrophosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF44">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF98103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="327" mass="37427" checksum="26AE58F4245EDF5A" modified="2024-11-27" version="1">MEELNSILQKTKDKSTQKEQDEILLQPFTYIQQIPGKQFRSELALAFNHWLLIPAEKLAQIGDIVQMLHNSSLLGVPVAHSIYGVASTINAANYALFLALEKVQLLGHPEATKVYTEQLLELHRGQGMEIYWRDSFTCPSESDYKLMTVRKTGGLFMLAIRLMQLFSANKEDYSKLTAILGLYFQIRDDYCNLSLKEYTENKSFAEDLTEGKFGFPVIHAVRSQKQDKQVLHILRQRTHDIEVKKYCISLLEKLGSFQYTRKVLETLDAEARAEVMRLGSNPYMDRLLNKLLSWKTSDSASITQSNQINHNNVTARNSPNQNTLNCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRF3</accession>
    <name>A0AAU9FRF3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Uracil phosphoribosyltransferase homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_06341</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRF3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000026">
        <property type="entry name" value="Uracil phosphoribosyltransferase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="10">
        <location>
            <begin position="66"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF98086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="258" mass="28446" checksum="FEE056721ADC41A9" modified="2024-11-27" version="1">MCTGSPSSNGSQPEEANATTEQQPQPEQEATEQPLRTPTHAIVGAAKAEEILAEYGSNLKLLECNSQVSELLTIIRDKNTTRSDFKFYADRLIRLVIEESLNQLPYTDCDVETPTGAIYEGLKYRSGNCGVSIIRSGEAMEQGLRDCCRSIRIGKILVESDANTHEARVVYARFPDDIGSRQVLLMYPIMSTGNTVLQAVNVLREHGVPEKCIILSNLFCTPVAARTVVNAFPQLKILTSELHPVAPNHFGQKYFGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRF6</accession>
    <name>A0AAU9FRF6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Uracil phosphoribosyltransferase homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_06341</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRF6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000026">
        <property type="entry name" value="Uracil phosphoribosyltransferase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="10">
        <location>
            <begin position="61"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF98084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="253" mass="28070" checksum="1CB3B7F8A0864726" modified="2024-11-27" version="1">MRSNGSQPEEANATTEQQPQPEQEATEQPLRTPTHAIVGAAKAEEILAEYGSNLKLLECNSQVSELLTIIRDKNTTRSDFKFYADRLIRLVIEESLNQLPYTDCDVETPTGAIYEGLKYRSGNCGVSIIRSGEAMEQGLRDCCRSIRIGKILVESDANTHEARVVYARFPDDIGSRQVLLMYPIMSTGNTVLQAVNVLREHGVPEKCIILSNLFCTPVAARTVVNAFPQLKILTSELHPVAPNHFGQKYFGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRG3</accession>
    <name>A0AAU9FRG3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">AF4/FMR2 family member lilli</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_06706</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRG3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097733">
        <property type="term" value="C:photoreceptor cell cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000094">
        <property type="entry name" value="protein C21orf2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 410-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849">
        <property type="entry name" value="LEUCINE RICH REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="157"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="341"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="253"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="267"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="395"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF98567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="469" mass="52915" checksum="425E50FD80214EF2" modified="2024-11-27" version="1">MTRLTEEMVIARAKQSDLGLITKLNCWGSDLTDVSIIKRMRGVEVLALSVNKISTLAPFEDCSKLQELYLRKNHIQDINEIAYLQNLPALKNLWLEENPCCEQSGSNYRAIVLRALPNLKKLDNVEVTQEEVDDALRRGGGGGGGGSVPEDEVYEDAYEARQQQRTSPQHSYPTHSPPPQQQAQQQQQYHPQTQQQPQQQQQQQQQRQSSSPIMREPLLLASPLGNHSSEGTNTHSASDLGGSQVLPVHGSKPPTPTKEPVHPPSPMYNTLTKEQRTSYQQYDRSPGSDQESSPNAGYREVRPAPIPPSISAHSMKEYYQSDRPAYPAHYRHSQTDLTEWEEHQQHQVPQVHHNPYGSQMLNHPQRRSVGPESGERFTGYRNGSARENGGDWDPEERPRSRRPEGRYSDSTNNVSASVANHYSGYHRRPINRSSNLLSATLCLVKELDYASLEVLEHAVRCRIDELATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRG7</accession>
    <name>A0AAU9FRG7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Formin-binding protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_06448</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FNBP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRG7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07653">
        <property type="entry name" value="F-BAR_CIP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11619">
        <property type="entry name" value="HR1_CIP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11911">
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Arthropoda</taxon>
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            <taxon>Endopterygota</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FNBP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRH4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd07653">
        <property type="entry name" value="F-BAR_CIP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11619">
        <property type="entry name" value="HR1_CIP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11911">
        <property type="entry name" value="SH3_CIP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000203">
        <property type="entry name" value="Cdc42-interacting protein 4, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000055">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057870">
        <property type="entry name" value="HR1_TOCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF12">
        <property type="entry name" value="CDC42-INTERACTING PROTEIN 4, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25610">
        <property type="entry name" value="HR1_TOCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11 12" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0728">SH3 domain</keyword>
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        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="16">
        <location>
            <begin position="335"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="525"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="416"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="122"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="342"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="416"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="461"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="484"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF98232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="591" mass="66415" checksum="309B440EC64C611E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRH6</accession>
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        <submittedName>
            <fullName evidence="9">Probable E3 ubiquitin-protein ligase HERC4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_06728</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="FunFam" id="3.30.2410.10:FF:000003">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
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        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
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        <property type="entry name" value="RCC1/BLIP-II"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR051709">
        <property type="entry name" value="Ub-ligase/GTPase-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622:SF76">
        <property type="entry name" value="HECT AND RLD DOMAIN CONTAINING E3 UBIQUITIN LIGASE 4, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="55"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="110"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="163"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="218"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="270"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="323"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="733"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="1027"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF98599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1059" mass="119101" checksum="7D24776A4A98A507" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRH8</accession>
    <name>A0AAU9FRH8_DROMD</name>
    <protein>
        <submittedName>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <strain evidence="4 5">RF1</strain>
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    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF98133.1"/>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FZV0</accession>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        </lineage>
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    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="17 18">RF1</strain>
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        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
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        </reaction>
    </comment>
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        <text evidence="2">Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.</text>
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        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19865">
        <property type="entry name" value="DSRM_STRBP_RED-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000044">
        <property type="entry name" value="Uncharacterized protein, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000049">
        <property type="entry name" value="Uncharacterized protein, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002466">
        <property type="entry name" value="A_deamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910:SF62">
        <property type="entry name" value="AT07585P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910">
        <property type="entry name" value="EUKARYOTE SPECIFIC DSRNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02137">
        <property type="entry name" value="A_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00552">
        <property type="entry name" value="ADEAMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50141">
        <property type="entry name" value="A_DEAMIN_EDITASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DRBM" evidence="3">
        <location>
            <begin position="55"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="3">
        <location>
            <begin position="225"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="A to I editase" evidence="4">
        <location>
            <begin position="358"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFG00957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="663" mass="73817" checksum="93A31FE7DA6E6058" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FZW8</accession>
    <name>A0AAU9FZW8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N(G)-N(G)-dimethylarginine dimethylaminohydrolase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_00946</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DDAH family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FZW8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016403">
        <property type="term" value="F:dimethylargininase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006525">
        <property type="term" value="P:arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000052">
        <property type="term" value="P:citrulline metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045429">
        <property type="term" value="P:positive regulation of nitric oxide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.75.10.10:FF:000004">
        <property type="entry name" value="N(G),N(G)-dimethylarginine dimethylaminohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033199">
        <property type="entry name" value="DDAH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12737:SF9">
        <property type="entry name" value="DIMETHYLARGININASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12737">
        <property type="entry name" value="DIMETHYLARGININE DIMETHYLAMINOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG01116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="268" mass="29877" checksum="6EAB4A160053BB90" modified="2024-11-27" version="1">MSPKYTHAIVARISEALLESGPFDVKLAKRQHEQYCTLLREIGLDVIELPPDDLLPEGVFVENSAVICNGVALIGRSDNAKRRREAASMAIILKKELDIPVIEIDDPHARLDGGDVLFTGREFFIGISGCTNEEGARAVAMAYPEYPVTPIRVNGSKRLKYYVTMAGPDVLCVSKSAPCQEIVKRMEREASFTYQKLTLPEESAANMLYINGTIVHRSPTEIPDAYKTLKEKIDIPTRNINISEFSQYSSGLTSSCLLLRRWKSIRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FZX2</accession>
    <name>A0AAU9FZX2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_01040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FZX2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="361"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="645"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="433"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG01243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="689" mass="76526" checksum="BA91DC04C744D8C9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FZX3</accession>
    <name>A0AAU9FZX3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein stunted</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_00978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ATPase epsilon family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FZX3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12153">
        <property type="entry name" value="F1-ATPase_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1620.20:FF:000005">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.20">
        <property type="entry name" value="ATP synthase, F1 complex, epsilon subunit superfamily, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006721">
        <property type="entry name" value="ATP_synth_F1_esu_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036742">
        <property type="entry name" value="ATP_synth_F1_esu_sf_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12448">
        <property type="entry name" value="ATP SYNTHASE EPSILON CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12448:SF0">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT EPSILON, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04627">
        <property type="entry name" value="ATP-synt_Eps"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48690">
        <property type="entry name" value="Epsilon subunit of mitochondrial F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG01158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="58" mass="6537" checksum="7C7719899BB851B2" modified="2024-11-27" version="1">MTNAWRAAGITYIQYSNIAARVLREALRTELRADAAKRDASHVKFTPWANGKPAHEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FZX6</accession>
    <name>A0AAU9FZX6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Neurobeachin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_00820</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    <sequence length="3669" mass="406440" checksum="21BF3ADD85206982" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3F0</accession>
    <name>A0AAU9G3F0_DROMD</name>
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        <recommendedName>
            <fullName evidence="9">DNA topoisomerase I</fullName>
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            <fullName evidence="9">DNA topoisomerase 1</fullName>
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        <name evidence="13" type="ORF">DMAD_01932</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
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    </reference>
    <comment type="function">
        <text evidence="9">Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
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            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
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            <location evidence="2">Nucleus</location>
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        <text evidence="3 8 9">Belongs to the type IB topoisomerase family.</text>
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        <property type="entry name" value="TopoI_DNA-bd_euk"/>
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        <property type="entry name" value="Topoisomer_I_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051062">
        <property type="entry name" value="Topoisomerase_IB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290:SF3">
        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290">
        <property type="entry name" value="DNA TOPOISOMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14370">
        <property type="entry name" value="Topo_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01028">
        <property type="entry name" value="Topoisom_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02919">
        <property type="entry name" value="Topoisom_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00416">
        <property type="entry name" value="EUTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00435">
        <property type="entry name" value="TOPEUc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46596">
        <property type="entry name" value="Eukaryotic DNA topoisomerase I, dispensable insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56741">
        <property type="entry name" value="Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TOPO_IB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52038">
        <property type="entry name" value="TOPO_IB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0799">Topoisomerase</keyword>
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        <location>
            <begin position="642"/>
            <end position="1017"/>
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        <location>
            <begin position="1"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="337"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="925"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="58"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="154"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="177"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="281"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="337"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="348"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="421"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="8">
        <location>
            <position position="1003"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG02430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1045" mass="118727" checksum="B31060B4DC5DA80F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3F4</accession>
    <name>A0AAU9G3F4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Dopamine D2-like receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_02117</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3F4"/>
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        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001591">
        <property type="term" value="F:dopamine neurotransmitter receptor activity, coupled via Gi/Go"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="7tmA_D2-like_dopamine_R"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CLUMA_CG012980, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000247">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
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        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF125">
        <property type="entry name" value="DOPAMINE D2-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="384"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="419"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="539"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="820"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="859"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="399"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="604"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="702"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="773"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="8"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="716"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="728"/>
            <end position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG02669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="898" mass="94466" checksum="1A22D1FD96980F0F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3F5</accession>
    <name>A0AAU9G3F5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 11">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="5 11">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_01670</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 11">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="5 11"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3F5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000097">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF59">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9 10 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="12">
        <location>
            <begin position="79"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG02064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="535" mass="58312" checksum="ABED963F74C6CEC9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3F8</accession>
    <name>A0AAU9G3F8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ELAV-like protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Protein ElrD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_01769</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RRM elav family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3F8"/>
    <dbReference type="SMR" id="A0AAU9G3F8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12650">
        <property type="entry name" value="RRM1_Hu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12652">
        <property type="entry name" value="RRM2_Hu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12377">
        <property type="entry name" value="RRM3_Hu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000006">
        <property type="entry name" value="ELAV-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000005">
        <property type="entry name" value="ELAV-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000480">
        <property type="entry name" value="Fne, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006548">
        <property type="entry name" value="ELAD_HU_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034775">
        <property type="entry name" value="Elav_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01661">
        <property type="entry name" value="ELAV_HUD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="26"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="127"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="270"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFG02197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="353" mass="38062" checksum="3EB29873A992224E" modified="2024-11-27" version="1">MTNAMDIVKNGSANGSVDGSNDESRTNLIVNYLPQTMTQEEMRSLFSSIGELESCKLVRDKVSVLPASLTALNPALQQGQSLGYGFVNYVRAEDAEKAVNTLNGLRLQNKVIKVSYARPSSESIKGANLYVSGLPKNLSQPDLEGMFASFGKIITSRILCDNISGLSKGVGFIRFDQRNEAERAIQELNGKTPKGYAEPITVKFANNPSNSAKAQIAPPLTAYLTPQAAAATRRLAGALPNAGRIRYSPLAGDLLANSILPGNAMTGSGWCIFVYNLAPETEENVLWQLFGPFGAVQSVKVIRDLQTSKCKGFGFVTMTNYDEAVVAIQSLNGYTLGNRVLQVSFKTNKTKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3G3</accession>
    <name>A0AAU9G3G3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Pneumococcal serine-rich repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_01942</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005221">
        <property type="term" value="F:intracellularly cyclic nucleotide-activated monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000004">
        <property type="entry name" value="Cyclic nucleotide-gated olfactory channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000126">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000058">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050866">
        <property type="entry name" value="CNG_cation_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG02316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1989" mass="207247" checksum="973E6247EAF6D1F2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3H0</accession>
    <name>A0AAU9G3H0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase family 92 protein</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_01782</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyltransferase 92 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3H0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008166">
        <property type="entry name" value="Glyco_transf_92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461:SF83">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01697">
        <property type="entry name" value="Glyco_transf_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG02212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="503" mass="57354" checksum="CE1554C831E46B43" modified="2024-11-27" version="1">MVYKLLRAWRTPLICVAGTLALLLVIQQLLDKSLAGHPSQEKQLKKETAVPKVHVAVDSPVDSPVDSPVVVATDLEAILRENGKHHWAQGSRRSHQCAPFPRYEDIVFKNPDFQWVVAGNLSYFLFGAYYDCRPAAPEVMVLAMVTTLKGPHPEAHCQLWYADGGQPDLVPVSSTKVGWYDVWGNVAGVAYPTMLSCVVPEPDIRVPQLVSLVFGSPCAPARNALVVVNAEVSQRPRPPPSTQQRLRTGVCVKFLDFPDRDMSARMVEWLELLRLLGVERVVAYDIGQLTANTSHTLAHYSQVDGLLQLRQHRLLAELPEHRGLRMCLTEVLMYNDCLYRNMYEFDYLGVFDVDEVIMPLGELQSYQLLLQELQRFDVNCTERTSFCFRNVYFPEELAADPALPQHFYMLSHVTRVAEHLDANSAIKCLHSPLYVTLTHNHFPMQWRESCGPMDVPVHFGQMQHYRHVDDLTTLTRPTPVRDDNILRFREQLLRNIHRRLGWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3H5</accession>
    <name>A0AAU9G3H5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cytoplasmic dynein 1 intermediate chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_02105</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3H5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000552">
        <property type="entry name" value="Cytoplasmic dynein 1 intermediate chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001109">
        <property type="entry name" value="Cytoplasmic dynein 1 intermediate chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025956">
        <property type="entry name" value="DYNC1I1/DYNC1I2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF22">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 INTERMEDIATE CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11540">
        <property type="entry name" value="Dynein_IC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="504"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="17"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG02644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="644" mass="72001" checksum="918454851CA425CB" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3H6</accession>
    <name>A0AAU9G3H6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphorylase kinase</fullName>
            <ecNumber evidence="2">2.7.11.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_01698</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 ATP + phosphorylase b = 2 ADP + phosphorylase a.</text>
            <dbReference type="EC" id="2.7.11.19"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.19" evidence="2"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3H6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005964">
        <property type="term" value="C:phosphorylase kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004689">
        <property type="term" value="F:phosphorylase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14093">
        <property type="entry name" value="STKc_PhKG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000138">
        <property type="entry name" value="Phosphorylase b kinase gamma catalytic chain, liver/testis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000149">
        <property type="entry name" value="phosphorylase b kinase gamma catalytic chain, liver/testis isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002291">
        <property type="entry name" value="Phosph_kin_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01049">
        <property type="entry name" value="PHOSPHBKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="7 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="23"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="417"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="436"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG02105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="557" mass="62935" checksum="DBD5F124CEEE3B16" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3H9</accession>
    <name>A0AAU9G3H9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_01832</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3H9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="RalA_RalB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative ras-related protein ral-a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
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        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG02277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="197" mass="22464" checksum="87A856E7A09F7E58" modified="2024-11-27" version="1">MSKKPTVGPALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLCVFSITDDESFQATQEFREQILRVKNDESIPFLLVGNKCDLNDKRKVPLSECQLRAQQWAVPYVETSAKTRENVDKVFYDLIRDISSRKKQRQTDVKQPPKPNSHCCQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G4C3</accession>
    <name>A0AAU9G4C3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Hormone receptor 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_01894</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07168">
        <property type="entry name" value="NR_DBD_DHR4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06953">
        <property type="entry name" value="NR_LBD_DHR4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000006">
        <property type="entry name" value="Nuclear receptor subfamily 5 group A member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050200">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48092">
        <property type="entry name" value="KNIRPS-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="11">
        <location>
            <begin position="998"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="12">
        <location>
            <begin position="1337"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="151"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="370"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="559"/>
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                <person name="Yamamoto D."/>
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        <location>
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    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="61"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00068395"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076663"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000304"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG06745.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="401" mass="44881" checksum="0D1CFB32E1A4FC8B" modified="2024-11-27" version="1">MPLFGKKDSGKKAKSKDMKELNKQVSIEEKYNLHGLLGTGAFSEVRLAESKDTPGDHFAVKIIDKKALKGKEESLENEIRVLRRLTHPNIVQLLETYEDKSKVYLVMELVTGGELFDRIVEKGSYTEKDASHLIRQILEAVDYMHEQGVVHRDLKPENLLYYSPEDDSKIMISDFGLSKMEDSGIMATACGTPGYVAPEVLAQKPYGKAVDVWSIGVISYILLCGYPPFYDENDANLFAQILKGEFEFDSPYWDEISESAKQFIKNLMCVTVEKRFTCKQALGHPWISGNEASSKNIHGTVSEQLKKNFAKSRWKQAYYAATVIRQMQRMALSSNSSAAADAANNDDSRKNSSNPITAIVPSVSASQTTHCKITSSNEPTTQTQNQEGRDMNNHQKTSKPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDH2</accession>
    <name>A0AAU9GDH2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycogen debranching enzyme</fullName>
            <ecNumber evidence="5">2.4.1.25</ecNumber>
            <ecNumber evidence="6">3.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Glycogen debrancher</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_04405</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Hydrolysis of (1-&gt;6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin.</text>
            <dbReference type="EC" id="3.2.1.33"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1-&gt;4)-alpha-D-glucan.</text>
            <dbReference type="EC" id="2.4.1.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the glycogen debranching enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.25" evidence="5"/>
    <dbReference type="EC" id="3.2.1.33" evidence="6"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004134">
        <property type="term" value="F:4-alpha-glucanotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004135">
        <property type="term" value="F:amylo-alpha-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11327">
        <property type="entry name" value="AmyAc_Glg_debranch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000108">
        <property type="entry name" value="glycogen debranching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000139">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000056">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010401">
        <property type="entry name" value="AGL/Gdb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032788">
        <property type="entry name" value="AGL_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029436">
        <property type="entry name" value="AGL_euk_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032792">
        <property type="entry name" value="AGL_glucanoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032790">
        <property type="entry name" value="GDE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006421">
        <property type="entry name" value="Glycogen_debranch_met"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01531">
        <property type="entry name" value="glyc_debranch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569:SF2">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06202">
        <property type="entry name" value="GDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14701">
        <property type="entry name" value="hDGE_amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14702">
        <property type="entry name" value="hGDE_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14699">
        <property type="entry name" value="hGDE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="15" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Eukaryotic glycogen debranching enzyme N-terminal" evidence="20">
        <location>
            <begin position="54"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme glucanotransferase" evidence="21">
        <location>
            <begin position="161"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme central" evidence="22">
        <location>
            <begin position="741"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme C-terminal" evidence="19">
        <location>
            <begin position="1079"/>
            <end position="1547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00025780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF06202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF14699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF14701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF14702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BFG05736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1560" mass="175272" checksum="918BE485BC1F7038" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDI1</accession>
    <name>A0AAU9GDI1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pneumococcal serine-rich repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_04747</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MTSS family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030031">
        <property type="term" value="P:cell projection assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07643">
        <property type="entry name" value="I-BAR_IMD_MIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000010">
        <property type="entry name" value="Metastasis suppressor 1, isoform CRA_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013606">
        <property type="entry name" value="I-BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030127">
        <property type="entry name" value="MTSS1/MTSS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708">
        <property type="entry name" value="ACTIN BUNDLING/MISSING IN METASTASIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708:SF4">
        <property type="entry name" value="FI21477P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08397">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51338">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMD" evidence="8">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="336"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="484"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="524"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="617"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="789"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="845"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1023"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1172"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1226"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1320"/>
            <end position="1405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1436"/>
            <end position="1467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1499"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1617"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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    <sequence length="1760" mass="187818" checksum="F9513BEAAA58B36D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDI6</accession>
    <name>A0AAU9GDI6_DROMD</name>
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        <recommendedName>
            <fullName evidence="13">Guanine nucleotide-binding protein G(s) subunit alpha</fullName>
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        <alternativeName>
            <fullName evidence="13">Adenylate cyclase-stimulating G alpha protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_04182</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
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    </reference>
    <comment type="function">
        <text evidence="10">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(s) protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 13">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
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            <location evidence="13">Cell membrane</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="1 13">Belongs to the G-alpha family. G(s) subfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="Gprotein_alpha_S"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6 11 13" id="KW-0342">GTP-binding</keyword>
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    <keyword evidence="5 12 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="382" mass="44701" checksum="7172AE17DDC320FB" modified="2024-11-27" version="1">MGCFGSPTSKQSDVNSEDSKSQKRRSDAISRQLQKDKQLYRATHRLLLLGAGESGKSTIVKQMRILHVDGFSETEKKQKIDDIKKNIRDAILTITGAMSTLNPPVALEKKENEPRVEYIQDYASSPDFNYPPEFYEHTEELWKDKGVLQTYERSNEYQLIDCAKYFLDRVSTIKNPNYTPNEQDILRCRVLTSGIFETRFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVTACSSYNMVLREDPTQNRLRESLDLFKSIWNNRWLRTISIILFLNKQDLLAEKIKAGKSKLSEYFSEFNKYQTPSDAIMESSDDPEVIRAKYFIRDEFLRISTASGDGKHYCYPHFTCAVDTENIKRVFNDCRDIIQRMHLRQYELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDI8</accession>
    <name>A0AAU9GDI8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GTP-binding protein RAD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_04416</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. RGK family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDI8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005246">
        <property type="term" value="F:calcium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000664">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051641">
        <property type="entry name" value="RGK_GTP-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45775">
        <property type="entry name" value="RAD, GEM/KIR FAMILY MEMBER 2, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45775:SF1">
        <property type="entry name" value="RAD, GEM_KIR FAMILY MEMBER 3, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="33"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="164"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG05751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="451" mass="49655" checksum="FAD62E1D34AC0A3A" modified="2024-11-27" version="1">MVDDISPMHHRMQKKTRSASICATGASIETVIHGLPTASGSATPEGGTPGYRRRRPATRSQSARITSGARSVRQRTKAQQQQQQQQHTLQEARSYCNSEPRLSETETPPQRRKLSQRRPQPHGHRKSNAFLDVPTMNMHHLRVNDDDEDVDRLRTFSASKGGIINRGDSFRRRRSRSNSLAPSSPMHARNGVGGLGGHVGGGSSQGLGSGYNGGGSSSNGFGHGQSAQENHQPVEFYRVEMLGSAGVGKQALVSQFRTSDCINAYDGPECDEAEQNISIILNGTESELKFLTGNPDSKDDLEEADAFLVVYSCIDKESFTRAKQILSRLQDMDLHRHRPIILVANKIDLARSRAVSAQDGKCLACAFGAKFIEVSVGINHNCDELLAGTLTQIRLKKDQNMLQGHRDGSSPAHWYKSRSVMLASMKARQMLTWILGKEDSKFKHCENLQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDI9</accession>
    <name>A0AAU9GDI9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3',5'-cyclic-AMP phosphodiesterase</fullName>
            <ecNumber evidence="4">3.1.4.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_04799</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.53" evidence="4"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDI9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000096">
        <property type="entry name" value="High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000002">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000555">
        <property type="entry name" value="Phosphodiesterase 8, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057304">
        <property type="entry name" value="PDE8-like_REC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23198">
        <property type="entry name" value="PDE8A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0114">cAMP</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="12">
        <location>
            <begin position="332"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="PDEase" evidence="13">
        <location>
            <begin position="578"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="128"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="660"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="660"/>
            <end position="664"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="664"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="702"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="703"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="703"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="703"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="819"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="819"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="871"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFG06254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="934" mass="103449" checksum="843D531111C58E1C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDJ3</accession>
    <name>A0AAU9GDJ3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coronin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_04754</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat coronin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDJ3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
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    <dbReference type="GO" id="GO:0051015">
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</entry>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
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        </source>
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        </subcellularLocation>
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        <text evidence="4">Belongs to the synaptopodin family.</text>
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</entry>
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        </source>
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        </source>
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</entry>
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        <name type="scientific">Drosophila madeirensis</name>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12 13">RF1</strain>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="536" mass="60798" checksum="2D22DC482D4E78E6" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
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        <name evidence="10" type="ORF">DMAD_07818</name>
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        <name type="scientific">Drosophila madeirensis</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10 11">RF1</strain>
        </source>
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        <subcellularLocation>
            <location evidence="6">Synapse</location>
            <location evidence="6">Synaptosome</location>
        </subcellularLocation>
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        <text evidence="1 7">Belongs to the SNAP-25 family.</text>
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        <property type="protein sequence ID" value="BFF88949.1"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="GOC"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Synaptosomal-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000018">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00835">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <keyword evidence="2" id="KW-0771">Synaptosome</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="9">
        <location>
            <begin position="27"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="9">
        <location>
            <begin position="149"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="58"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF88949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="213" mass="23741" checksum="3CC6A128235A9A1D" modified="2024-11-27" version="1">MPADPSEEIPGPQVPKTELEELQISAQGVADESLESTRRMLALCEESKEAGIRTLVALDDQGEQLDRIEEGMDQINADMREAEKNLSGMEKCCGICVLPCNKSQSFKEDDGTWKGNDDGKVVNNQPQRVMDDRNGMMAQAGYIGRITNDAREDEMEENMGQVNTMIGNLRNMALDMGSELENQNRQVDRINRKGESNEARIAVANQRAHQLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EX32</accession>
    <name>A0AAU9EX32_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_08198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MYG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EX32"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003226">
        <property type="entry name" value="MYG1_exonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11215">
        <property type="entry name" value="METAL DEPENDENT HYDROLASE - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11215:SF1">
        <property type="entry name" value="MYG1 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03690">
        <property type="entry name" value="MYG1_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF89439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="344" mass="39157" checksum="C7B2304B651CD907" modified="2024-11-27" version="1">MADASPPKRPTPIWIGTHSGTFHCDEVVACFMLRQLPEYENAEIFRSRDNTALREKCDIIVDVGGVYDHAKRWYDHHQTSFKETFNILRPEVAKDFNIRLSSAGLIYTHYGERVIESILKRERNVQLSPDNLQLAFVQIYRNFIRELDAIDNGEVMFEGGEPRYGIHSHLSARVGRLNPSWQDTNVDIEQRFKQAMDLAGREFVDNVLEVACSWIPARDHVRDALKEAKSVYPTGEILLLTTFCPWKAHLADVEKEYQVEGVPKLIIFSDGTGWRVAGVPLSPVSFVGRKFLPTSWRGKRDEELSELAGVKDLIFVHHSGFIGGAKTKEAALAMAKKSLEWKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EX34</accession>
    <name>A0AAU9EX34_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GDNF/GAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_09422</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GDNFR family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EX34"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057681">
        <property type="entry name" value="DUF7921"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059035">
        <property type="entry name" value="Fn1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016017">
        <property type="entry name" value="GDNF/GAS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037193">
        <property type="entry name" value="GDNF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003438">
        <property type="entry name" value="GDNF_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269">
        <property type="entry name" value="GDNF RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269:SF12">
        <property type="entry name" value="GLIAL CELL LINE-DERIVED NEUROTROPHIC FAMILY RECEPTOR-LIKE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25537">
        <property type="entry name" value="DUF7921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25868">
        <property type="entry name" value="Fn1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02351">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00907">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110035">
        <property type="entry name" value="GDNF receptor-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GDNF/GAS1" evidence="9">
        <location>
            <begin position="27"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="9">
        <location>
            <begin position="125"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="9">
        <location>
            <begin position="353"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="9">
        <location>
            <begin position="462"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="567"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="567"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="581"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="615"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="666"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF91045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1006" mass="109950" checksum="D5618FF98E1A6966" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EX39</accession>
    <name>A0AAU9EX39_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Heterogeneous nuclear ribonucleoprotein Q</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_09800</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EX39"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21065">
        <property type="entry name" value="NURR_Syncrip-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12249">
        <property type="entry name" value="RRM1_hnRNPR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12250">
        <property type="entry name" value="RRM2_hnRNPR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12251">
        <property type="entry name" value="RRM3_hnRNPR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000175">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000024">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein q isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000213">
        <property type="entry name" value="Uncharacterized protein, isoform R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041337">
        <property type="entry name" value="hnRNP_Q_AcD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006535">
        <property type="entry name" value="HnRNP_R/Q_splicing_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01648">
        <property type="entry name" value="hnRNP-R-Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21245">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18360">
        <property type="entry name" value="hnRNP_Q_AcD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="180"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="261"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="356"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="553"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="553"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF91543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="725" mass="79520" checksum="15E479B0490BB2D0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EX81</accession>
    <name>A0AAU9EX81_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Regulator of gene activity</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_09832</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EX81"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000036">
        <property type="term" value="P:regulation of stem cell population maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1020:FF:000005">
        <property type="entry name" value="Regena, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="5">
        <location>
            <begin position="425"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="28"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="149"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="340"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="188"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="222"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="352"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF91588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="565" mass="57871" checksum="2B81649079F102AA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EX99</accession>
    <name>A0AAU9EX99_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Diacylglycerol kinase</fullName>
            <shortName evidence="15">DAG kinase</shortName>
            <ecNumber evidence="15">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_08245</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="15"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20800">
        <property type="entry name" value="C1_DGK_typeII_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20852">
        <property type="entry name" value="C1_DGK_typeII_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09507">
        <property type="entry name" value="SAM_DGK-delta-eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000021">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000001">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000002">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000029">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000001">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054474">
        <property type="entry name" value="DGKD_4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF109">
        <property type="entry name" value="DIACYLGLYCEROL KINASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22944">
        <property type="entry name" value="DGKD_4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 15 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="18">
        <location>
            <begin position="33"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="18">
        <location>
            <begin position="105"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="DAGKc" evidence="20">
        <location>
            <begin position="187"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="19">
        <location>
            <begin position="1691"/>
            <end position="1754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="440"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="619"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="858"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="956"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="677"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="440"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="455"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="629"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="956"/>
            <end position="966"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="BFF89499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1754" mass="194797" checksum="8089E4D121AC82D7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0B3</accession>
    <name>A0AAU9F0B3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamine--fructose-6-phosphate transaminase (isomerizing)</fullName>
            <ecNumber evidence="3">2.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_07791</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="3"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F0B3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000001">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="8">
        <location>
            <begin position="2"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="9">
        <location>
            <begin position="343"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="9">
        <location>
            <begin position="514"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF88909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="665" mass="74637" checksum="BE2C1F49B704695F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0B6</accession>
    <name>A0AAU9F0B6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Protein crumbs</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_08937</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the Crumbs protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048667">
        <property type="term" value="P:cell morphogenesis involved in neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009792">
        <property type="term" value="P:embryo development ending in birth or egg hatching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002064">
        <property type="term" value="P:epithelial cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060562">
        <property type="term" value="P:epithelial tube morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048592">
        <property type="term" value="P:eye morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048871">
        <property type="term" value="P:multicellular organismal-level homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051093">
        <property type="term" value="P:negative regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003002">
        <property type="term" value="P:regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048638">
        <property type="term" value="P:regulation of developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008593">
        <property type="term" value="P:regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061326">
        <property type="term" value="P:renal tubule development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003008">
        <property type="term" value="P:system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000185">
        <property type="entry name" value="basement membrane-specific heparan sulfate proteoglycan core protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000208">
        <property type="entry name" value="Crumbs 2, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000039">
        <property type="entry name" value="Crumbs cell polarity complex component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000496">
        <property type="entry name" value="Crumbs, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000716">
        <property type="entry name" value="Crumbs, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000143">
        <property type="entry name" value="Crumbs, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000181">
        <property type="entry name" value="Crumbs, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000192">
        <property type="entry name" value="Crumbs, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000172">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000004">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000279">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000173">
        <property type="entry name" value="Neurogenic locus notch protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000565">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="26"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="28"/>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="15"/>
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        <property type="entry name" value="Growth factor receptor domain"/>
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        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="15"/>
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        <property type="match status" value="23"/>
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        <property type="match status" value="16"/>
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    <sequence length="2209" mass="241531" checksum="F6946D9BFA66AB7A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0F6</accession>
    <name>A0AAU9F0F6_DROMD</name>
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        <recommendedName>
            <fullName evidence="7">CBS domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="8" type="ORF">DMAD_07832</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.</text>
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        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase gamma subunit family.</text>
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        <property type="entry name" value="LD22662P"/>
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    <keyword evidence="3 5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="892"/>
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            <begin position="1046"/>
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        <location>
            <begin position="1117"/>
            <end position="1177"/>
        </location>
    </feature>
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            <begin position="1"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
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            <begin position="421"/>
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        <location>
            <begin position="1203"/>
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        <location>
            <begin position="49"/>
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    </feature>
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            <begin position="65"/>
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    </feature>
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        <location>
            <begin position="76"/>
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            <begin position="115"/>
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            <begin position="143"/>
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            <begin position="172"/>
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        <location>
            <begin position="1358"/>
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    </feature>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51371"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1383" mass="150791" checksum="53E97C88F4A077A0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0H8</accession>
    <name>A0AAU9F0H8_DROMD</name>
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        <submittedName>
            <fullName evidence="17">Dynein heavy chain 5 axonemal</fullName>
        </submittedName>
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        <name evidence="17" type="ORF">DMAD_07853</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
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        <text evidence="2">Belongs to the dynein heavy chain family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Dynein_AAA5_ext"/>
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        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="Dynein_C"/>
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        <property type="entry name" value="Dynein_heavy"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2037"/>
            <end position="2184"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2319"/>
            <end position="2448"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2696"/>
            <end position="2869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="951"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3322"/>
            <end position="3349"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3542"/>
            <end position="3590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="979"/>
            <end position="994"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF88999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="4563" mass="522485" checksum="A721D85FD90A43AB" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0I5</accession>
    <name>A0AAU9F0I5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bestrophin homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_09342</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Forms chloride channels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the anion channel-forming bestrophin (TC 1.A.46) family. Calcium-sensitive chloride channel subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F0I5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000615">
        <property type="entry name" value="Bestrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021134">
        <property type="entry name" value="Bestrophin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10736">
        <property type="entry name" value="BESTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10736:SF65">
        <property type="entry name" value="BESTROPHIN 1, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01062">
        <property type="entry name" value="Bestrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0868">Chloride</keyword>
    <keyword evidence="6" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="6" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="439"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="522"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="572"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="462"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="582"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF90924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="717" mass="78763" checksum="028E76E2CD95A9C3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0I9</accession>
    <name>A0AAU9F0I9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Synapse-associated protein of 47 kDa</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_08415</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F0I9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048172">
        <property type="term" value="P:regulation of short-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3970.10:FF:000001">
        <property type="entry name" value="synapse-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051494">
        <property type="entry name" value="BSD_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019:SF6">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD" evidence="3">
        <location>
            <begin position="295"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="478"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="515"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="527"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF89721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="542" mass="56302" checksum="AEC6C31F8FF034A5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F8R1</accession>
    <name>A0AAU9F8R1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Aminopeptidase N</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_10207</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F8R1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000013">
        <property type="entry name" value="Aminopeptidase N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000008">
        <property type="entry name" value="aminopeptidase N isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF294">
        <property type="entry name" value="THYROTROPIN-RELEASING HORMONE-DEGRADING ECTOENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 19" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 12" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="18">
        <location>
            <begin position="172"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="16">
        <location>
            <begin position="411"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="17">
        <location>
            <begin position="724"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="486"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="509"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="13">
        <location>
            <position position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFF92069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1072" mass="121985" checksum="451DAB2B040220A9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F8R3</accession>
    <name>A0AAU9F8R3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uridine phosphorylase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_10201</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PNP/UDP phosphorylase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F8R3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004850">
        <property type="term" value="F:uridine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006218">
        <property type="term" value="P:uridine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17763">
        <property type="entry name" value="UP_hUPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010059">
        <property type="entry name" value="Uridine_phosphorylase_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01719">
        <property type="entry name" value="euk_UDPppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691:SF11">
        <property type="entry name" value="FI09636P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691">
        <property type="entry name" value="URIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="3">
        <location>
            <begin position="60"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="145"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR610059-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF92061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="347" mass="38912" checksum="F103A905D97C9519" modified="2024-11-27" version="1">MSLLLTGISNSLSDEELDEYSDGTVKLRNSNIELMDQDILYHLALGSESHDLQEMFGDVKFVCMGGTPKRMENFAHFIMDEIGYKLPAGTQLQDISAYSYRYSMYKVGPVLCVSHGMGTPSVSILMHEMIKLMYHAKCIDPIFIRIGTCGGIGVEGGTVIITEDALDGQLRNSHEFTILGETVHRPAKLDKKLARELKSLHSADDPYDTIIGKTQCTNDFYEGQGRLDGAFCEFSENDKMAYLEKLRDHGVVNIEMESTIFAALTHHAGIKAAVVCVALLNRLNGDQVNAPKEVMHEWQQRPQILVSRYIRKVLTRDGQLKNLFGHQGSIKSPRRFKLVQQESQAHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F8W8</accession>
    <name>A0AAU9F8W8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Protein lap4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_10248</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Insecta</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <begin position="10"/>
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        </location>
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        <location>
            <begin position="27"/>
            <end position="42"/>
        </location>
    </feature>
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        </location>
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        </source>
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        </source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1811" mass="192326" checksum="015EFF076899E75D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F933</accession>
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        <submittedName>
            <fullName evidence="7">Transcription initiation factor IIA subunit 1</fullName>
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        <name evidence="7" type="ORF">DMAD_10309</name>
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        <name type="scientific">Drosophila madeirensis</name>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
        </source>
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    </evidence>
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            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F974</accession>
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        <recommendedName>
            <fullName evidence="2">DUF4773 domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="ORF">DMAD_10318</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
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            <begin position="25"/>
            <end position="166"/>
        </location>
    </feature>
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        <location>
            <begin position="44"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF92217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="166" mass="17367" checksum="63A327914E8BDA19" modified="2024-11-27" version="1">MWSPSSVLCLVLGTLVSLSGITAADATNGTNLGFITIPSQAQVCQCSLSLSCNCCQSITVNAINETKTLCLTLKINVLSASVDVGVTMDGNSVTKFTISTQTPPTACLPLLSVVTPLDICLKLNIKMAGLTGLKACPTFYTSYDSNNVLSYDFPCLQLGMEGISLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAU9F975_DROMD</name>
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        <submittedName>
            <fullName evidence="8">Axin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_10341</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="term" value="C:beta-catenin destruction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000025">
        <property type="entry name" value="Axin, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.130:FF:000004">
        <property type="entry name" value="Axin, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.196.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043581">
        <property type="entry name" value="Axin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014936">
        <property type="entry name" value="Axin_b-cat-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001158">
        <property type="entry name" value="DIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038207">
        <property type="entry name" value="DIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024066">
        <property type="entry name" value="RGS_subdom1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46102">
        <property type="entry name" value="AXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46102:SF2">
        <property type="entry name" value="AXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08833">
        <property type="entry name" value="Axin_b-cat_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00778">
        <property type="entry name" value="DIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00021">
        <property type="entry name" value="DAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50841">
        <property type="entry name" value="DIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="RGS" evidence="6">
        <location>
            <begin position="57"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="DIX" evidence="7">
        <location>
            <begin position="635"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="258"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="470"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="553"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="190"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="263"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="521"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="567"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="587"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF92245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="717" mass="78844" checksum="0DDFEE6B9FD7759C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F986</accession>
    <name>A0AAU9F986_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA repair protein RAD51 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_10330</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the RecA family. RAD51 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F986"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070192">
        <property type="term" value="P:chromosome organization involved in meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000730">
        <property type="term" value="P:DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042148">
        <property type="term" value="P:DNA strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990426">
        <property type="term" value="P:mitotic recombination-dependent replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19513">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000092">
        <property type="entry name" value="DNA repair protein Rad51 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011941">
        <property type="entry name" value="DNA_recomb/repair_Rad51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003301">
        <property type="entry name" value="PRK04301.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02239">
        <property type="entry name" value="recomb_RAD51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF39">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="Rad51 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="8">
        <location>
            <begin position="92"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 2" evidence="9">
        <location>
            <begin position="271"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF92233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="336" mass="35936" checksum="99EE351BD05C92BD" modified="2024-11-27" version="1">MEALTNSVEEEEESGPLNINKLIGGNITAKDIKLLQMASLHTVDSVANATRKSLLAIPGLGGSKVDQIIAAATKLVPIGFLSARTFHQMRADVVMLTTGSKELDKLLGGGIETGSITEIFGEFRCGKTQICHTLAVTCQLPISQKGGEGKCLYIDTESTFRPERLAAIAQRFGLNETGVLDNVACARAYNTDQQTKLIQMAAGMLFDTRYSLVIVDSIMALYRSDYTGRGELAARQNHLGLCVRLLQRLADEFGVAVVITNQVTAVLDGGGGMFVADAKKPVGGHILAHASTTRLYLRKGKGDTRICKVYDSPCLPESEGMFAILPDGIGDAKDGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F993</accession>
    <name>A0AAU9F993_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">acid phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_10352</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="3"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F993"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF211">
        <property type="entry name" value="PROSTATIC ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00778">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043953150" description="acid phosphatase" evidence="9">
        <location>
            <begin position="31"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="407"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF92259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="447" mass="51075" checksum="066975D8D69A07E1" modified="2024-11-27" version="1">MWNHSHQLCCAKALLVIGVLCVLSFGIGNAVHIPTYGSSEGETRPPPDQLATLPGELKFAHVIFRHGDRTPLDPYPTDPWNNRKFWPTGWGQLTNLGKEQHYELGKWLRNRYKSLLGSRYTNEDIFVQSTDVDRTLMSAQSDLAGLYEPQGDDIWNPRIDWQPVPVHTVPEKDDSILAAKASCPAYDYELATLKASSEFQALYVRYRELLSYLTQNSGRLVKSFLDAQYLNNTLFIEKLYNMTLPVWAEKVYGKEELTYVSNFAFSIATFTRSMARLKTGPLLKDIFERFDKKLNNQLKPDRSLWIYSAHDTTIANVLNSLKLFELHSPPYAACIMLEMRVDDSNTPLVSVFYKNTTAEPLPLDIPGCGLSCPLKTLVKVYQDVLPVNWERECKRSTMMMTYEEANLGAATGILIFIITVLLCASYGLMVYYRRRHYNLYTSYSQMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F995</accession>
    <name>A0AAU9F995_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Keratin type I cytoskeletal 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_10351</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F995"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0416">Keratin</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="311"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF92257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="327" mass="34426" checksum="7C96FC2FD4E4627F" modified="2024-11-27" version="1">MADAAIAALNGTLFKGVSIVVEAGRPKERSRNGAGSRVPPSVGGGGGSGRRSLEGQSGGSNNRRDGGGSAGGRYGSEGGFNSNGSQFGPIRNETNFRQQRNAPYSKGPPQQQTSNESPQGYRNKFSVGSNGGRNGDDGGRRFQHRDNSDGAGPSNANRGAPQGPRRNLKNSPSNNNNDFLAGNSSFNNPRGVRQDRRGFALPVEQQQMTFGVQQNRFRNGSNSGSGNRHSSGVNGADPQGRRGSYNDRGGFNARRGAANNEHNRPQRPGGAGNKPGHDQSGGLHGSYQTEFPPLGRHEGGRGGGAVHRNRFNGPRPGPNNMVGNRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAC6</accession>
    <name>A0AAU9FAC6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_10667</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FAC6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 7" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF92657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="388" mass="44629" checksum="A5E5F1CC847CDEFD" modified="2024-11-27" version="1">MTIFLNTLYGIFWGRFSFKRSKFIISKPVAVYSILVGLSFAVYHVLQVYEEFTSGRITSRDTIKIYCYMNVCVCLLNYVTQWAITGPVVRFQNSVPLFPVANALDTSMMMVWRALLHCVLKMMVCPLIVYVTLVLYQHRAHPDMEWTIIASVRTMMPLIISNQVNNCFFGGLVIANLIVAAINKRIRSIVREANMLQTPLQMVLLKPYYRMQRFCELADNLDELALKYVVTASCSKGYLRFTDWSMIVSMVMNLIGITVGFYNQYLAIADYYINEDQPYDLHQAIVQLVFLSVPFMELILVSRISSQMLLETKKTGDLLQRIDLQHADVRFKQVVNSFWLQVSTFDYKLMPLGLLELNSSLVNTVFSAVTGFLLILIQSDLTLRFSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAD1</accession>
    <name>A0AAU9FAD1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Leucine-rich melanocyte differentiation-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DMAD_10663</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FAD1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043313">
        <property type="entry name" value="LRMDA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46282">
        <property type="entry name" value="LEUCINE-RICH MELANOCYTE DIFFERENTIATION-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46282:SF2">
        <property type="entry name" value="LEUCINE-RICH MELANOCYTE DIFFERENTIATION-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF92651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="181" mass="21243" checksum="9C19F37CA80D8854" modified="2024-11-27" version="1">MQTLEDSDTQLILVQQNLRSLPQELVKQHAEHVELLDLSHNCLRDLSWLGEFEQLRHLVLDNNRLHEAQLRTLTQSLPQLEVLMLNKNEFSDLPSTMRLIRRLFPNLQYLSLHGNPICPDGLELQPFSGYLRYDYAYYSNYIAQSLSKLKFLDHGLVQRTYQYESFPISSYGKQLIKTTSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAD2</accession>
    <name>A0AAU9FAD2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_10616</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF92595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="416"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="464"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="553"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="586"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="657"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="751"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="779"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="698"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="51"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="705"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF92595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="851" mass="97180" checksum="A41DEF74CBF0F053" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAH4</accession>
    <name>A0AAU9FAH4_DROMD</name>
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        <submittedName>
            <fullName evidence="7">Protein spaetzle</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_10648</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:central nervous system formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:innate immune response"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
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        <property type="entry name" value="Spaetzle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052444">
        <property type="entry name" value="Spz/Toll_ligand-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23199">
        <property type="entry name" value="NEUROTROPHIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23199:SF12">
        <property type="entry name" value="NEUROTROPHIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16077">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043482342" evidence="5">
        <location>
            <begin position="25"/>
            <end position="397"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
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        </location>
    </feature>
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            <end position="217"/>
        </location>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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    </evidence>
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            <dbReference type="Pfam" id="PF16077"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="397" mass="45109" checksum="5DEED2C91BDA35FF" modified="2024-11-27" version="1">MPPMWYQLIKILVLLFALFTTIVCINSEISKRREAEAAAAAATAAATLAAVEAAAGHARPNHNTDMSDFTSDNLMHILRSKAKEHQGKEHQEAAKTVDHPELEPAELPAKDLNQTINDIFKVYDAPGVHIFNTTTTDYAPLMPIHTMIDQSQPQQPIQPLKQSKPQPPPSHYHQYHSLSQQDQYFKGTNPATVTMPDQRDEAQRTRQPPENPIVFPDSAVMHQMRTPEPPLIKRDIPQCVEDQDNHKSKSFCTEVKNYPDLSRIKGKLSLNFSNFFSDPEPVPMDISSRLGPNDEVFLCRSHRRYLYPKSGLKSDNTWQFIVNNEEFKQGILIEECENEDMPCDYSSSFPQRYKPICKQHYAMRMLASIRNTSGEELDVAQESFKIPSCCKCVLKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAI9</accession>
    <name>A0AAU9FAI9_DROMD</name>
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        <submittedName>
            <fullName evidence="5">Probable Rho GTPase-activating protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_10666</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Ral GTPase-activating protein subunit alpha-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
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        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR027107">
        <property type="entry name" value="Tuberin/Ral-act_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063:SF11">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN CG5521-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063">
        <property type="entry name" value="TUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20412">
        <property type="entry name" value="RALGAPB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="4">
        <location>
            <begin position="1653"/>
            <end position="1860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="400"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="641"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="794"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1450"/>
            <end position="1471"/>
        </location>
    </feature>
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        <location>
            <begin position="1577"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1946"/>
            <end position="2002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="400"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="650"/>
            <end position="668"/>
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                <person name="Yamamoto D."/>
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</entry>
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            <authorList>
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                <person name="Yamamoto D."/>
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</entry>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Binds covalently through a thioester bond to the pathogen surface resulting in pathogen clearance.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Modulates negatively TGFB1 signaling in keratinocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterodimer of a TEP1-N chain and an TEP1-C chain non-covalently linked. Forms a complex composed of TEP1-N and TEP1-C heterodimer, LRIM1 and APL1C; the interaction stabilizes TEP1-N and TEP1-C heterodimer, prevents its binding to tissues while circulating in the hemolymph and protects the thioester bond from hydrolysis. Mature TEP1 and to a lesser extent full-length TEP1 interact with SPCLIP1; the interaction is induced by microbial infection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterodimer; disulfide-linked. Interacts with TGFB1 and TGFBR1. Forms a heteromeric complex with TGFBR1, TGFBR2 and TGFBR3 in a ligand-independent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000001">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1940:FF:000003">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000155">
        <property type="entry name" value="complement C3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2950:FF:000001">
        <property type="entry name" value="Thioester-containing protein 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.690:FF:000003">
        <property type="entry name" value="Thioester-containing protein 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.20.50.160:FF:000002">
        <property type="entry name" value="Thioester-containing protein 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040839">
        <property type="entry name" value="MG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF136">
        <property type="entry name" value="CD109 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17789">
        <property type="entry name" value="MG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0882">Thioester bond</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043448594" description="CD109 antigen" evidence="20">
        <location>
            <begin position="22"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="21">
        <location>
            <begin position="440"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="22">
        <location>
            <begin position="675"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="23">
        <location>
            <begin position="1288"/>
            <end position="1378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="BFF95672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1400" mass="154610" checksum="2111458FBDA6FC7F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FIT7</accession>
    <name>A0AAU9FIT7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger protein ush</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_12984</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FIT7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003087">
        <property type="entry name" value="U-shaped, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059121">
        <property type="entry name" value="CCHC_ZFPM2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039746">
        <property type="entry name" value="FOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034731">
        <property type="entry name" value="Znf_CCHC_FOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12958">
        <property type="entry name" value="FRIEND OF GATA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12958:SF3">
        <property type="entry name" value="ZINC FINGER PROTEIN USH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25445">
        <property type="entry name" value="CCHC_ZFPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13909">
        <property type="entry name" value="zf-H2C2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51810">
        <property type="entry name" value="ZF_CCHC_FOG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
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        <property type="protein sequence ID" value="BFF95541.1"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:animal organ development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
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        <property type="entry name" value="Ig"/>
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        <property type="entry name" value="PTKc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_I-set"/>
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        <property type="entry name" value="Ig_sub2"/>
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        <property type="entry name" value="Immunoglobulin_dom"/>
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        <property type="entry name" value="Tyr_kinase_AS"/>
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        <property type="entry name" value="PDGF- AND VEGF-RECEPTOR RELATED, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="6"/>
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        <property type="match status" value="4"/>
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        <property type="match status" value="5"/>
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        <property type="match status" value="5"/>
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        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
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    <keyword evidence="12 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 21" id="KW-0675">Receptor</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
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    <feature type="chain" id="PRO_5043594340" description="receptor protein-tyrosine kinase" evidence="18">
        <location>
            <begin position="25"/>
            <end position="1547"/>
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        <location>
            <begin position="801"/>
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        <location>
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        <location>
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            <end position="473"/>
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    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="478"/>
            <end position="574"/>
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        <location>
            <begin position="580"/>
            <end position="692"/>
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    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="701"/>
            <end position="791"/>
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        <location>
            <begin position="876"/>
            <end position="1282"/>
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        <location>
            <begin position="1036"/>
            <end position="1073"/>
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    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1347"/>
            <end position="1367"/>
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    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
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            <end position="1547"/>
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        <location>
            <begin position="1036"/>
            <end position="1045"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1349"/>
            <end position="1361"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1404"/>
            <end position="1423"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1525"/>
            <end position="1537"/>
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            <position position="1146"/>
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        <location>
            <begin position="883"/>
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        <location>
            <position position="910"/>
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        <ligand>
            <name>ATP</name>
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        <location>
            <position position="1151"/>
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        <ligand>
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        <location>
            <position position="1164"/>
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        <ligand>
            <name>Mg(2+)</name>
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        <location>
            <position position="1290"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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        <source>
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        </source>
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        <source>
            <dbReference type="EMBL" id="BFF95541.1"/>
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        <source>
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    </evidence>
    <sequence length="1547" mass="174138" checksum="20F09BCD3F6DA307" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="12">Nuclear receptor subfamily 4 group A member 4</fullName>
        </alternativeName>
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="16 17">RF1</strain>
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        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
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        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NHR-like_dom_sf"/>
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        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
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                <person name="Yamamoto D."/>
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</entry>
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                <person name="Yamamoto D."/>
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</entry>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="21 22">RF1</strain>
        </source>
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</entry>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <topology evidence="1">Single-pass membrane protein</topology>
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    <sequence length="3571" mass="385560" checksum="C312DB77995FE007" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJ55</accession>
    <name>A0AAU9FJ55_DROMD</name>
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        <recommendedName>
            <fullName evidence="18">CD109 antigen</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">TEP1-F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_13024</name>
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    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Binds covalently through a thioester bond to the pathogen surface resulting in pathogen clearance.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Modulates negatively TGFB1 signaling in keratinocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterodimer of a TEP1-N chain and an TEP1-C chain non-covalently linked. Forms a complex composed of TEP1-N and TEP1-C heterodimer, LRIM1 and APL1C; the interaction stabilizes TEP1-N and TEP1-C heterodimer, prevents its binding to tissues while circulating in the hemolymph and protects the thioester bond from hydrolysis. Mature TEP1 and to a lesser extent full-length TEP1 interact with SPCLIP1; the interaction is induced by microbial infection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterodimer; disulfide-linked. Interacts with TGFB1 and TGFBR1. Forms a heteromeric complex with TGFBR1, TGFBR2 and TGFBR3 in a ligand-independent manner.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
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        <property type="term" value="P:immune system process"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="complement C3 isoform X1"/>
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        <property type="entry name" value="Thioester-containing protein 2, isoform B"/>
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        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
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        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
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        <location>
            <begin position="1"/>
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        </location>
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            <begin position="22"/>
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            <begin position="440"/>
            <end position="574"/>
        </location>
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            <begin position="672"/>
            <end position="765"/>
        </location>
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            <begin position="1287"/>
            <end position="1377"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        </source>
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        </source>
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        </source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00078071"/>
        </source>
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        <source>
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        <source>
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        </source>
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        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
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        <source>
            <dbReference type="EMBL" id="BFF95673.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1399" mass="154967" checksum="E69097EC9EABC91B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJ59</accession>
    <name>A0AAU9FJ59_DROMD</name>
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        <submittedName>
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        <name evidence="9" type="ORF">DMAD_13078</name>
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        <name type="scientific">Drosophila madeirensis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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        </subcellularLocation>
    </comment>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJ97</accession>
    <name>A0AAU9FJ97_DROMD</name>
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        <recommendedName>
            <fullName evidence="5">Dynein regulatory complex protein 10</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">DMAD_13062</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJ97"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="343"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF95718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="431" mass="50175" checksum="4B64CFCA2A7772C8" modified="2024-11-27" version="1">MSTMLSRGAKPLQAQVGFKHAFPPEGFFPLPPAESHLCRDAYLKALLAPFESVSDDGSEEPEDDGLPKKDLFATKKFDIEFMMISRRRTTMWKPTKYRPSAINVNLRASAYDLSALVKARYVRLLEGFGISCINYLLEKIIKKVEIMRTVPPEGTDPKKTIFGWSCHEYYERFKKHSDTIYLDTFLFDKTAAELQDLKYNVDLEEIVRLMRSLKNDLTEENDLCQGFMALLRDIQVDIMEVLAKPNEDMYTEHDNLSTQLFAARQGKSHDPKMISKHRALKKEQQYLMDYKAPIETRYQINWIRDKLEQKDYEDASQVKIIQDELDKLKELTATDTAVWNAANQSYDEDMETAENNVQMTLNKLNKCKDDLRSYQEKVESFHLKIAETRAKIAKEEAQELQKLRILEEKQARKSELKEKAKPKGKKEKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRI4</accession>
    <name>A0AAU9FRI4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_06407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRI4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF98170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="146" mass="16069" checksum="800D81BD3F209301" modified="2024-11-27" version="1">MPNLSQSAQAEQHDEEYHEDPQEEHQHKEEQQQQKPPQRAKSRHKAPDDTGHPFDVDATNANATTQSTGRSPMPPAIAAAPVPVLPPPPPPPPPPSRHRTKALPLLYLVGGALQQRQFANPISYLGGPRLQPRSNRNSIESILSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRI6</accession>
    <name>A0AAU9FRI6_DROMD</name>
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        <recommendedName>
            <fullName evidence="4">3-phosphoinositide-dependent protein kinase 1</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_06578</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRI6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901701">
        <property type="term" value="P:cellular response to oxygen-containing compound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048638">
        <property type="term" value="P:regulation of developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01262">
        <property type="entry name" value="PH_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05581">
        <property type="entry name" value="STKc_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000191">
        <property type="entry name" value="3-phosphoinositide-dependent protein kinase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000405">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000587">
        <property type="entry name" value="Phosphoinositide-dependent kinase 1, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000324">
        <property type="entry name" value="Phosphoinositide-dependent kinase 1, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033931">
        <property type="entry name" value="PDK1-typ_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039046">
        <property type="entry name" value="PDPK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF163">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14593">
        <property type="entry name" value="PH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="242"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="211"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="400"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="761"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="803"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="400"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="412"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="441"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="454"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="764"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="810"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="832"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF98398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="847" mass="94070" checksum="E6FC97A181D3085E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRI7</accession>
    <name>A0AAU9FRI7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Arginine-glutamic acid dipeptide repeats protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_06738</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="SANT_MTA3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000052">
        <property type="entry name" value="Arginine-glutamic acid dipeptide (RE) repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.50:FF:000004">
        <property type="entry name" value="arginine-glutamic acid dipeptide repeats protein-like"/>
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    <sequence length="2111" mass="224500" checksum="1C281E88353B5F70" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13 14">RF1</strain>
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</entry>
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            <begin position="1037"/>
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    <evidence type="ECO:0000256" key="14">
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1063" mass="114756" checksum="6B0C51F30F688791" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRJ9</accession>
    <name>A0AAU9FRJ9_DROMD</name>
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        <submittedName>
            <fullName evidence="13">HBS1-like protein</fullName>
        </submittedName>
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        <name evidence="13" type="ORF">DMAD_06643</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
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        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
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            <dbReference type="ChEBI" id="CHEBI:43474"/>
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            <dbReference type="Rhea" id="RHEA:19670"/>
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        <subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="EFTu-like_2"/>
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        <property type="entry name" value="HBS1-like_N"/>
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        <property type="entry name" value="HBS1-like_N_sf"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
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        <property type="entry name" value="Transl_B-barrel_sf"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="Translation proteins"/>
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        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Tr-type G" evidence="12">
        <location>
            <begin position="225"/>
            <end position="448"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="168"/>
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    </feature>
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        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF98483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="650" mass="72005" checksum="2271B5209C199A98" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRK3</accession>
    <name>A0AAU9FRK3_DROMD</name>
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        <recommendedName>
            <fullName evidence="5">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_06258</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRK3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
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        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:Golgi organization"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="TANGO2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985">
        <property type="entry name" value="SER/THR-RICH PROTEIN T10 IN DGCR REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985:SF8">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05742">
        <property type="entry name" value="TANGO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFG01170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="292" mass="32236" checksum="AACFBB00F55B3EC1" modified="2024-11-27" version="1">MCVIFFYTNSNPQPGGYRLILASNRDEFFARKTVAATEWPNADHVVGGIDLEPGREGGTWLAIGSDSASASASGVFKVGALLNLTGEPKPRDAVGRGTIVADFVSQFDEPTNSLNFNQSLLQDCTKYSAFNFVSIEIGPRSVPAQIRLLSNVPPTLNEFEEGKCYGFGNSLPGTPFEKVRFGQQQFARIVEQHGADSAEALSAQLLQLLKNKTKFWPDAELKRRAPNWGEELSALNVHIVDRGYGSRTHSIILVDANNQMHFFEENFEPEMNPQELWNPVTVVHKHLGGQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FZY3</accession>
    <name>A0AAU9FZY3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Coronin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_01105</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">F-actin regulator involved in anterograde Golgi to endosome transport: upon ubiquitination via 'Lys-33'-linked ubiquitin chains by the BCR(KLHL20) E3 ubiquitin ligase complex, interacts with EPS15 and localizes to the trans-Golgi network, where it promotes actin polymerization, thereby facilitating post-Golgi trafficking. May play a role in the maintenance of the Golgi apparatus morphology.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the WD repeat coronin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Coronin"/>
    </dbReference>
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        <property type="entry name" value="DUF1899"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
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        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CORONIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="WD40_4"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01167">
        <property type="entry name" value="DUF1900"/>
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    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0677">Repeat</keyword>
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    <feature type="domain" description="DUF1899" evidence="11">
        <location>
            <begin position="4"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="75"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1899" evidence="11">
        <location>
            <begin position="843"/>
            <end position="908"/>
        </location>
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    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="967"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="1010"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="418"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="461"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="514"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="632"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="681"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="418"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="469"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="514"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="536"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="585"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="645"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="708"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="720"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="738"/>
            <end position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU280818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG01324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1306" mass="143512" checksum="F0FA120398C10920" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FZY4</accession>
    <name>A0AAU9FZY4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Disintegrin and metalloproteinase domain-containing protein unc-71</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_00969</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
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    <dbReference type="FunFam" id="4.10.70.10:FF:000001">
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    </dbReference>
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        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADAM_Cys-rich"/>
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    <dbReference type="InterPro" id="IPR018358">
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    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF237">
        <property type="entry name" value="MIND-MELD, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08516">
        <property type="entry name" value="ADAM_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00289">
        <property type="entry name" value="DISINTEGRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00427">
        <property type="entry name" value="DISINTEGRIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
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    <sequence length="1454" mass="159923" checksum="137D011331525E39" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FZZ0</accession>
    <name>A0AAU9FZZ0_DROMD</name>
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        <recommendedName>
            <fullName evidence="19">Nuclear factor of activated T-cells 5</fullName>
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        <alternativeName>
            <fullName evidence="20">T-cell transcription factor NFAT5</fullName>
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        <name evidence="23" type="ORF">DMAD_00991</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="23 24">RF1</strain>
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    </reference>
    <comment type="function">
        <text evidence="17">Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3'. Mediates the transcriptional response to hypertonicity. Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17. Also involved in the DNA damage response by preventing formation of R-loops; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer when bound to DNA, completely encircles its DNA target. Interacts with CIDEC; this interaction is direct and retains NFAT5 in the cytoplasm. Does not bind with Fos and Jun transcription factors. Interacts with DDX5 and DDX17; this interaction leads to DDX5/DDX17 recruitment to LNC2 and S100A4 promoters and NFAT5-mediated DDX5/DDX17-enhanced transactivation.</text>
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        <subcellularLocation>
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            <location evidence="3">Cytoplasm</location>
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        <subcellularLocation>
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        <property type="entry name" value="NFAT NUCLEAR FACTOR, ISOFORM B"/>
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        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
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    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00055141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00065799"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00072227"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00080722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BFG01173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1278" mass="135157" checksum="40E405F1D18BAAF8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FZZ1</accession>
    <name>A0AAU9FZZ1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_00971</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FZZ1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16744">
        <property type="entry name" value="RING-HC_RNF185"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000062">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF185"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045103">
        <property type="entry name" value="RNF5/RNF185-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12313">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="104"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="159"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG01146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="256" mass="27524" checksum="5C1F11DB50FF1750" modified="2024-11-27" version="1">METPKLATVPEHANLSYTGSSSVNTHMRPSGRSTLTVSEEEGAATSTSYSFTGSSLAGGNIADLNKSVDAGSAKSEPGLNAETKDATGNSSSDKEHAEESLYECNICLDTAKDAVVSMCGHLFCWPCLHQWLLTRPSRKLCPVCKAAVDKDKVIPLYGRNSTRQEDPRNKVPPRPAGLRTEPEPAPGFPGFGFGDGFHMSFGIGAFPFGFITSSLNFGEPRPAAANRGTTQYEDEQTLSKLFLYLAFLCIGWLLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FZZ5</accession>
    <name>A0AAU9FZZ5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Troponin T skeletal muscle</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_01021</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin T family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FZZ5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000003">
        <property type="entry name" value="Troponin T isoform 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027707">
        <property type="entry name" value="TNNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521">
        <property type="entry name" value="TROPONIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521:SF1">
        <property type="entry name" value="TROPONIN T, SKELETAL MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="53"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="218"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="274"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="53"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="76"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="274"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="322"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFG01215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="387" mass="46348" checksum="CA804693BAE45A69" modified="2024-11-27" version="1">MSDDEEYTSSEEEEVVEETREETGEGDPEFIKRQDQKRSDLDEQLKEYISEWRKQRSKEEDELKKLKEKQAKRKISRAEEEQKMAQRKKEEEERRVREAEEKKQREIEEKRMRLEEAEKKRQAMLQAMKDKDKKGPNFTIAKKDAGLGLSSAAMERNKTKEQLEEEKKISLSFRIKPLAIEGFGESKLREKAQELWELIVKLETEKYDLEERQKRQDYDLKELKERQKQQLRHKALKKGLDPEALTGKYPPKIQVASKYERRVDTRSYDDKKKLFEGGFDEITKDSNEKIWNEKKDQYTGRQKSKLPKWFGERPGKKAGEPETPEGEEDAKADEDIVEDDEEVEEEVVEEEDEEAEEDEEEEEEEEEEEEEEEEEEEEEEEEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3E7</accession>
    <name>A0AAU9G3E7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MAP kinase-activating death domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_02358</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MADD family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="term" value="F:kinase activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="MAP KINASE-ACTIVATING DEATH DOMAIN PROTEIN"/>
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        <property type="entry name" value="MAP-KINASE ACTIVATING DEATH DOMAIN PROTEIN MADD /DENN/AEX-3 C.ELEGANS"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
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            <begin position="1880"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3E9</accession>
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        <recommendedName>
            <fullName evidence="1">Kelch-like protein diablo</fullName>
        </recommendedName>
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        <name evidence="7" type="ORF">DMAD_01810</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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        <text evidence="4">Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. May have a role in synapse differentiation and growth.</text>
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        <property type="entry name" value="LD33804P"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3I2</accession>
    <name>A0AAU9G3I2_DROMD</name>
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        <recommendedName>
            <fullName evidence="1">BTB/POZ domain-containing protein 9</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">DMAD_01794</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3I2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048512">
        <property type="term" value="P:circadian behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14822">
        <property type="entry name" value="BACK_BTBD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18287">
        <property type="entry name" value="BTB_POZ_BTBD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000005">
        <property type="entry name" value="BTB/POZ domain-containing protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000051">
        <property type="entry name" value="BTB/POZ domain-containing protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000042">
        <property type="entry name" value="BTB/POZ domain-containing protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052407">
        <property type="entry name" value="BTB_POZ_domain_cont_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034091">
        <property type="entry name" value="BTBD9_BACK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46306">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46306:SF1">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="50"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="574"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="674"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="574"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="641"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="682"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="700"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG02227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="759" mass="85874" checksum="F883C75DC774D5F2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3I5</accession>
    <name>A0AAU9G3I5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Amyloid-beta-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_01871</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the APP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3I5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.770:FF:000002">
        <property type="entry name" value="beta-amyloid-like protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.570.10:FF:000002">
        <property type="entry name" value="beta-amyloid-like protein isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.770">
        <property type="entry name" value="Amyloid precursor protein, E2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.140">
        <property type="entry name" value="Amyloidogenic glycoprotein, copper-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.570.10">
        <property type="entry name" value="Amyloidogenic glycoprotein, heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036669">
        <property type="entry name" value="Amyloid_Cu-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008155">
        <property type="entry name" value="Amyloid_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011178">
        <property type="entry name" value="Amyloid_glyco_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024329">
        <property type="entry name" value="Amyloid_glyco_E2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008154">
        <property type="entry name" value="Amyloid_glyco_extra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015849">
        <property type="entry name" value="Amyloid_glyco_heparin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036454">
        <property type="entry name" value="Amyloid_glyco_heparin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019745">
        <property type="entry name" value="Amyloid_glyco_intracell_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019543">
        <property type="entry name" value="APP_amyloid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019744">
        <property type="entry name" value="APP_CUBD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036176">
        <property type="entry name" value="E2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103">
        <property type="entry name" value="ALZHEIMER'S DISEASE BETA-AMYLOID RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103:SF15">
        <property type="entry name" value="AMYLOID-BETA-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10515">
        <property type="entry name" value="APP_amyloid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12924">
        <property type="entry name" value="APP_Cu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12925">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02177">
        <property type="entry name" value="APP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00006">
        <property type="entry name" value="A4_EXTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56491">
        <property type="entry name" value="A heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89811">
        <property type="entry name" value="Amyloid beta a4 protein copper binding domain (domain 2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109843">
        <property type="entry name" value="CAPPD, an extracellular domain of amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00319">
        <property type="entry name" value="APP_CUBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51869">
        <property type="entry name" value="APP_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51870">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00320">
        <property type="entry name" value="APP_INTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043706522" evidence="12">
        <location>
            <begin position="31"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="854"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="domain" description="E1" evidence="13">
        <location>
            <begin position="33"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="E2" evidence="14">
        <location>
            <begin position="433"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="GFLD subdomain" evidence="8">
        <location>
            <begin position="33"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="CuBD subdomain" evidence="8">
        <location>
            <begin position="145"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="274"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="708"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="661"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="298"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="714"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="147"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="158"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="172"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG02331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="929" mass="102341" checksum="2B9D563B213ED464" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3I8</accession>
    <name>A0AAU9G3I8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_01692</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16845"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3I8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006101">
        <property type="term" value="P:citrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06105">
        <property type="entry name" value="ScCit1-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.230.10:FF:000001">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.580.10:FF:000001">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010109">
        <property type="entry name" value="Citrate_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01793">
        <property type="entry name" value="cit_synth_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007128">
        <property type="entry name" value="PRK09569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF8">
        <property type="entry name" value="CITRATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="active site" evidence="8">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR610109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG02096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="464" mass="51609" checksum="D2513698BECEB0A2" modified="2024-11-27" version="1">MSLYRISARKLADAQKLPNVGSYVRLISADGRSLRDVLNAKVPEEQERVKNFRKQHGATKLGETTIDMMYGGMRGIKALVTETSVLDADEGIRFRGLSIPECQKVLPAAPGGTEPLPEGLFWLLLTGEVPTQSQVQQLSREWAERAALPQHVVTMLNNMPTSLHPMSQLAAAVTALNHDSKFAKAYSEGVHKSKYWEYVYEDSMDLIAKLPVVAATIYCNTYRGGKGSRSIDSSLDWSANFVKMLGYDNAQFTELMRLYLTIHSDHEGGNVSAHTVHLVGSALSDPYLSFAAGMNGLAGPLHGLANQEVLVWLRKLQKEAGNNPSEDQLKDYIWKTLKSGQVVPGYGHAVLRKTDPRYTCQREFALKHLPEDELFQLVSKIYKVVPPILTETGKVKNPWPNVDAHSGVLLQYYGMKEMNYYTVLFGVSRALGVLASLVWDRALGLPIERPKSYSTDLLVKMVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3J2</accession>
    <name>A0AAU9G3J2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glutathione hydrolase 1 proenzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_01806</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3J2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.130:FF:000001">
        <property type="entry name" value="Gamma-glutamyltransferase 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.40:FF:000001">
        <property type="entry name" value="Gamma-glutamyltranspeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF9">
        <property type="entry name" value="RE13973P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1199">Hemostasis impairing toxin</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-1202">Platelet aggregation activating toxin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0800">Toxin</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="130"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="143"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="612"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="630"/>
            <end position="632"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="654"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="680"/>
            <end position="681"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="703"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00084097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFG02242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="800" mass="84988" checksum="2BE3D8FBDE8446BC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3J4</accession>
    <name>A0AAU9G3J4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Oligopeptide transporter 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_01843</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3J4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006857">
        <property type="term" value="P:oligopeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Diptera</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="16 17">RF1</strain>
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    </dbReference>
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        <property type="entry name" value="PROTEIN SIDEKICK-LIKE PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
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    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="2082"/>
            <end position="2104"/>
        </location>
    </feature>
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        <location>
            <begin position="89"/>
            <end position="172"/>
        </location>
    </feature>
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        <location>
            <begin position="278"/>
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        </location>
    </feature>
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        <location>
            <begin position="376"/>
            <end position="462"/>
        </location>
    </feature>
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        <location>
            <begin position="535"/>
            <end position="621"/>
        </location>
    </feature>
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        <location>
            <begin position="626"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="722"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="839"/>
            <end position="936"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3K5</accession>
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            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
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        <name evidence="17" type="ORF">DMAD_02158</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="17 18">RF1</strain>
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            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="3070"/>
            <end position="3081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="3115"/>
            <end position="3126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="3127"/>
            <end position="3149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="3174"/>
            <end position="3183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="3191"/>
            <end position="3201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="3308"/>
            <end position="3319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="3386"/>
            <end position="3420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="3429"/>
            <end position="3446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="3534"/>
            <end position="3545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="3587"/>
            <end position="3599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="3656"/>
            <end position="3673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="3674"/>
            <end position="3688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="3696"/>
            <end position="3705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="3739"/>
            <end position="3752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="4680"/>
            <end position="4702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="4707"/>
            <end position="4718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="4787"/>
            <end position="4803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="4819"/>
            <end position="4839"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="12">
        <location>
            <position position="5254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG02729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="5287" mass="569870" checksum="E43B4DB3E100D12F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3K9</accession>
    <name>A0AAU9G3K9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Amyloid-beta-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_01871</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the APP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3K9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.770:FF:000002">
        <property type="entry name" value="beta-amyloid-like protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.570.10:FF:000002">
        <property type="entry name" value="beta-amyloid-like protein isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.770">
        <property type="entry name" value="Amyloid precursor protein, E2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.140">
        <property type="entry name" value="Amyloidogenic glycoprotein, copper-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.570.10">
        <property type="entry name" value="Amyloidogenic glycoprotein, heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036669">
        <property type="entry name" value="Amyloid_Cu-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008155">
        <property type="entry name" value="Amyloid_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011178">
        <property type="entry name" value="Amyloid_glyco_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024329">
        <property type="entry name" value="Amyloid_glyco_E2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008154">
        <property type="entry name" value="Amyloid_glyco_extra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015849">
        <property type="entry name" value="Amyloid_glyco_heparin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036454">
        <property type="entry name" value="Amyloid_glyco_heparin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019745">
        <property type="entry name" value="Amyloid_glyco_intracell_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019543">
        <property type="entry name" value="APP_amyloid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019744">
        <property type="entry name" value="APP_CUBD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036176">
        <property type="entry name" value="E2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103">
        <property type="entry name" value="ALZHEIMER'S DISEASE BETA-AMYLOID RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103:SF15">
        <property type="entry name" value="AMYLOID-BETA-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10515">
        <property type="entry name" value="APP_amyloid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12924">
        <property type="entry name" value="APP_Cu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12925">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02177">
        <property type="entry name" value="APP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00006">
        <property type="entry name" value="A4_EXTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56491">
        <property type="entry name" value="A heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89811">
        <property type="entry name" value="Amyloid beta a4 protein copper binding domain (domain 2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109843">
        <property type="entry name" value="CAPPD, an extracellular domain of amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00319">
        <property type="entry name" value="APP_CUBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51869">
        <property type="entry name" value="APP_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51870">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00320">
        <property type="entry name" value="APP_INTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043403822" evidence="12">
        <location>
            <begin position="31"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="853"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="domain" description="E1" evidence="13">
        <location>
            <begin position="33"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="E2" evidence="14">
        <location>
            <begin position="432"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="GFLD subdomain" evidence="8">
        <location>
            <begin position="33"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="CuBD subdomain" evidence="8">
        <location>
            <begin position="144"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="273"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="707"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="660"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="297"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="713"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="146"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="157"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="171"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG02326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="928" mass="102253" checksum="112440FDA335CE0B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3L0</accession>
    <name>A0AAU9G3L0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Neurogenic protein mastermind</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_01855</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.320.30:FF:000001">
        <property type="entry name" value="Myelin transcription factor 1-like, a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002515">
        <property type="entry name" value="Znf_C2H2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036060">
        <property type="entry name" value="Znf_C2H2C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816:SF15">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01530">
        <property type="entry name" value="zf-C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103637">
        <property type="entry name" value="CCHHC domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51802">
        <property type="entry name" value="ZF_CCHHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="314"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="375"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="496"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="646"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="875"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1118"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1315"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1383"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1556"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="377"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="496"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="513"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="565"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="611"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="647"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="672"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="905"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="915"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1162"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1184"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1315"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1341"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1399"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1423"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1452"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1574"/>
            <end position="1590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1591"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1616"/>
            <end position="1631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG02307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1684" mass="175524" checksum="838338EA28CD1660" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3L4</accession>
    <name>A0AAU9G3L4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fibrinogen C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_02426</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Lectin involved in innate immunity. Agglutinates all types of human erythrocytes, Gram-positive and Gram-negative bacteria. Has a stronger agglutinating activity towards Gram-negative bacteria than towards Gram-positive bacteria. Specifically recognizes acetyl group-containing substances on agglutinated cells. The hemagglutinating activity was inhibited by EDTA, acetyl group-containing mono- and disaccharides, N-acetyl derivatives of amino acids, other acetyl group-containing substances, propionamide and benzamide. Enhances the antimicrobial activity of big defensin against Gram-positive bacteria but not against Gram-negative bacteria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3L4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005577">
        <property type="term" value="C:fibrinogen complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034116">
        <property type="term" value="P:positive regulation of heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF6">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="10">
        <location>
            <begin position="559"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="467"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG03083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="796" mass="89648" checksum="38752B5459E14864" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3L7</accession>
    <name>A0AAU9G3L7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_01734</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cell membrane</location>
            <topology evidence="1 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
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        </source>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <sequence length="2159" mass="241310" checksum="4B9AD5753D8053CA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3L8</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
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        <source>
            <dbReference type="EMBL" id="BFG02744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="148" mass="16415" checksum="426CCA13C8369C33" modified="2024-11-27" version="1">MLLKENDELMNDILRQLEDEAKTKRQQELIEKSAKKPKVPRENLTVESETVAALQKKSGEVPYKQTASKAQGIAPINQRKVNNIKIKPLGKSAANGAEMVPEEPGLFKVPKVPAKRKPRKVDNIKIKALPKIASDKAGPEEPQLFLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDF6</accession>
    <name>A0AAU9GDF6_DROMD</name>
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        <recommendedName>
            <fullName evidence="6">Epoxide hydrolase</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="9" type="ORF">DMAD_04574</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes juvenile hormone hydrolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>1-(4-methoxyphenyl)-N-methyl-N-[(3-methyloxetan-3-yl)methyl]methanamine + H2O = 2-{[(4-methoxybenzyl)(methyl)amino]methyl}-2-methylpropane-1,3-diol</text>
            <dbReference type="Rhea" id="RHEA:55764"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:139161"/>
            <dbReference type="ChEBI" id="CHEBI:139164"/>
            <dbReference type="EC" id="3.3.2.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cis-stilbene oxide + H2O = (1R,2R)-hydrobenzoin</text>
            <dbReference type="Rhea" id="RHEA:23900"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:50004"/>
            <dbReference type="ChEBI" id="CHEBI:50014"/>
            <dbReference type="EC" id="3.3.2.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the peptidase S33 family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFG05968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033961">
        <property type="term" value="F:cis-stilbene-oxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:epoxide metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
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        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016292">
        <property type="entry name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661:SF35">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661">
        <property type="entry name" value="EPOXIDE HYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001112">
        <property type="entry name" value="Epoxide_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epoxide hydrolase N-terminal" evidence="8">
        <location>
            <begin position="58"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG05968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="468" mass="53370" checksum="D465AFADB62AF4C0" modified="2024-11-27" version="1">MKCLIIFGLIVAVFGAFVGYGYLVFTELTRPLPKPEISDNTYWGPGDGKDYVPDKRVNEFRLSVPQAEIEDLRQQLNRTLRLTDPLDGISFEYGFNSHALEQFVDYWREKYLTKWDERQEFFNKFKQYTTEIQGLNIHFIREKASEEAAAKKHVYPLLLLHGWPGSVREFYDIIPMLTNDQNVTDYAFEVVAPSLVGYGWSDAASRPGFNAAEMATVMRNLMLRLGHKKFFIQGGDWGSIIGSNLATLYPENVIGYHSNMCVLNSPLAILKGIYGTYFSEKIIPSRFFYDHHFPVWDKYAELLQESGYFHIQATKPDTIGAALTTSPVGLAAYILEKFQTCTNPGVKQDFGAIVTVFGLEPVLDNLMVYYLTNSITTAVRFYAENVSKAYTSLQLDRVPTPVPMGCSRFRYDLPSVTDWQLRDKFPNLKHSMYFQQGSHFAALEMPAMLYQDFTAFVGKIGLHGEKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDF7</accession>
    <name>A0AAU9GDF7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">RNA-binding protein MEX3B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_13648</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22423">
        <property type="entry name" value="KH-I_MEX3_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KH-I_MEX3_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16518">
        <property type="entry name" value="RING-HC_MEX3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mex-3 RNA-binding family member D"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KH-I_MEX3_rpt1"/>
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        <property type="entry name" value="KH-I_MEX3_rpt2"/>
    </dbReference>
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        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
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        <property type="entry name" value="MEX3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23285:SF7">
        <property type="entry name" value="LD09246P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23285">
        <property type="entry name" value="RING FINGER AND KH DOMAIN CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="568"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG06730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="619" mass="66188" checksum="449A75956F241631" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDF9</accession>
    <name>A0AAU9GDF9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Inner nuclear membrane protein Man1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_04211</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDF9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12934">
        <property type="entry name" value="LEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.40:FF:000001">
        <property type="entry name" value="LEM domain containing 2, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1180">
        <property type="entry name" value="MAN1, winged-helix domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052277">
        <property type="entry name" value="INM_ESCRT-Associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011015">
        <property type="entry name" value="LEM/LEM-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003887">
        <property type="entry name" value="LEM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018996">
        <property type="entry name" value="Man1/Src1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041885">
        <property type="entry name" value="MAN1_winged_helix_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13428:SF12">
        <property type="entry name" value="INNER NUCLEAR MEMBRANE PROTEIN MAN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13428">
        <property type="entry name" value="INNER NUCLEAR MEMBRANE PROTEIN MAN1 LEM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03020">
        <property type="entry name" value="LEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09402">
        <property type="entry name" value="MSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00540">
        <property type="entry name" value="LEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63451">
        <property type="entry name" value="LEM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50954">
        <property type="entry name" value="LEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="393"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="LEM" evidence="8">
        <location>
            <begin position="3"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG05486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="616" mass="70282" checksum="2429A90688CCEB72" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDK6</accession>
    <name>A0AAU9GDK6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_13612</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDK6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09445">
        <property type="entry name" value="LIM_Mical_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24206">
        <property type="entry name" value="OS06G0237300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="67"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="522"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="637"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="552"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG06632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="656" mass="75647" checksum="66066297A71ADC18" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDK7</accession>
    <name>A0AAU9GDK7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Microtubule-associated protein RP/EB family member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_04765</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAPRE family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDK7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1430:FF:000005">
        <property type="entry name" value="Eb1, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000007">
        <property type="entry name" value="Microtubule-associated protein, RP/EB family, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036133">
        <property type="entry name" value="EB1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140612">
        <property type="entry name" value="EB1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="14"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="12">
        <location>
            <begin position="212"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="148"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG06199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="294" mass="32697" checksum="1DF1EA7D2E8AAA72" modified="2024-11-27" version="1">MAVNVYSTNVTSENLSRHDMLAWVNDCLQSQFSKIEELCTGAAYCQFMDMLFPNSVPVKRVKFRTNLEHEYIQNFKILQAGFKKMSVDKIIPIDKLIKGRFQDNFEFLQWFKKFFDANYDGRDYDASGVREGAPMGFGSGAVKSLPGTGGGGSGGVASSYRRTPATAARPTTTATAKPTVSKVLPRTSSAVTHSRVATATNITGTVKKNDANHAVNTQQIDELSNQVMDMRLNLEGLEKERDFYFSKLRDIEILCQEAEEGETTPLVQKILDILYATEDGFAPPDDAPPEEEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDK9</accession>
    <name>A0AAU9GDK9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytoplasmic phosphatidylinositol transfer protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Retinal degeneration B homolog beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_04251</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the PtdIns transfer protein family. PI transfer class IIB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDK9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000011">
        <property type="entry name" value="cytoplasmic phosphatidylinositol transfer protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001666">
        <property type="entry name" value="PI_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055261">
        <property type="entry name" value="PI_transfer_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658:SF54">
        <property type="entry name" value="CYTOPLASMIC PHOSPHATIDYLINOSITOL TRANSFER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02121">
        <property type="entry name" value="IP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00391">
        <property type="entry name" value="PITRANSFER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="3" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Phosphatidylinositol transfer protein N-terminal" evidence="8">
        <location>
            <begin position="3"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="240"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="240"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="260"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00068698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00082927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG05540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="276" mass="32119" checksum="E2E4D72F9B5AC2CE" modified="2024-11-27" version="1">MVLTKEYRVCMPLTVEEYKIGQLYMIARHSLEQSEEGEGVEVVENKPCEDDVHGKGQYTEKHIHLSSRLPYWVQAICPRVFYVIEKSWNYYPYTLTGEWCSFIPKLNVLIKTKYEDNNGTTDNCLDLSEEELKLRSVDHLDIAFDEFSPKHYKREEDPKFFKSIKTNRGPLVEGWRETDKPIMCSYKVVHASFEVWGLQTKVEDFIQRGIRDILLLGHRQAFAWLDEWHGMTLEDVRDYERQKQAETNEKVQAAGDGPTPNATGSNGRNTPTQDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDL6</accession>
    <name>A0AAU9GDL6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">ATP-citrate synthase</fullName>
            <ecNumber evidence="19">2.3.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">ATP-citrate (pro-S-)-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Citrate cleavage enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_04256</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate in multiple biochemical reactions in protein, carbohydrate and lipid metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>oxaloacetate + acetyl-CoA + ADP + phosphate = citrate + ATP + CoA</text>
            <dbReference type="Rhea" id="RHEA:21160"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.3.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5 19">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.8" evidence="19"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDL6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003878">
        <property type="term" value="F:ATP citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006101">
        <property type="term" value="P:citrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06100">
        <property type="entry name" value="CCL_ACL-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.230.10:FF:000004">
        <property type="entry name" value="ATP-citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000003">
        <property type="entry name" value="ATP-citrate synthase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000004">
        <property type="entry name" value="ATP-citrate synthase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.110:FF:000003">
        <property type="entry name" value="ATP-citrate synthase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000024">
        <property type="entry name" value="Probable ATP-citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014608">
        <property type="entry name" value="ATP-citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032263">
        <property type="entry name" value="Citrate-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056749">
        <property type="entry name" value="Citrate_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23118">
        <property type="entry name" value="ATP-CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23118:SF42">
        <property type="entry name" value="ATP-CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16114">
        <property type="entry name" value="Citrate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24948">
        <property type="entry name" value="Citrate_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036511">
        <property type="entry name" value="ATP_citrt_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8 19" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="17 19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="CoA-binding" evidence="22">
        <location>
            <begin position="489"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="460"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="471"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="20">
        <location>
            <position position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00093367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR036511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR036511-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BFG05546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1097" mass="120193" checksum="136F9D9A512D3B5C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDN0</accession>
    <name>A0AAU9GDN0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Bedraggled</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_04267</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
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        <source>
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        <source>
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    <sequence length="1368" mass="150932" checksum="2FC21998B9D1539B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDN4</accession>
    <name>A0AAU9GDN4_DROMD</name>
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        <recommendedName>
            <fullName evidence="2">calcium/calmodulin-dependent protein kinase</fullName>
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        </recommendedName>
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        <name evidence="16" type="ORF">DMAD_13627</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
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    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:456216"/>
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        </reaction>
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    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome dot"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Ca/CaM-dep_prot_kinase-assoc"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="7 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="14"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
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            <end position="341"/>
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    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="43"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG06662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="489" mass="55399" checksum="1D012F99C87BC51A" modified="2024-11-27" version="1">MAAPAACTRFSDNYDIKEELGKGAFSIVKRCVQKSTGFEFAAKIINTKKLTARDFQKLEREARICRKLHHPNIVRLHDSIQEENYHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESVNHCHQNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGDHQAWFGFAGTPGYLSPEVLKKEPYGKSVDIWACGVILYILLVGYPPFWDEDQHRLYSQIKAGAYDYPSPEWDTVTPEAKNLINQMLTVNPNKRITASEALKHPWICQRERVASVVHRQETVDCLKKFNARRKLKGAILTTMLATRNFSSRSMITKKGDGSQVKESTDSSSTTLEDDDIKARRQEIIKITEQLIEAINSGDFDGYTKICDPHLTAFEPEALGNLVEGIDFHKFYFENVLGKNCKAINTTILNPHVHLLGEEAACIAYVRLTQYIDKQGHAHTHQSEETRVWHKRDNKWQNVHFHRSASGKISGATTFDFIPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDN6</accession>
    <name>A0AAU9GDN6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Receptor expression-enhancing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_04271</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDN6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071782">
        <property type="term" value="C:endoplasmic reticulum tubular network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF117">
        <property type="entry name" value="LP05237P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043414971" description="Receptor expression-enhancing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="500"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG05570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="551" mass="62400" checksum="FB66E8CE566F035E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDN7</accession>
    <name>A0AAU9GDN7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Guanylate kinase-associated protein mars</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_04652</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPAP family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDN7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031616">
        <property type="term" value="C:spindle pole centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051642">
        <property type="term" value="P:centrosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005026">
        <property type="entry name" value="SAPAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12353:SF1">
        <property type="entry name" value="DISKS LARGE-ASSOCIATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12353">
        <property type="entry name" value="DISKS LARGE-ASSOCIATED PROTEIN DAP SAP90/PSD-95-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03359">
        <property type="entry name" value="GKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="385"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="459"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="541"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="784"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="858"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="906"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="173"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="236"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="268"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="475"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="503"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="550"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="784"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="867"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="906"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="942"/>
            <end position="958"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG06058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="972" mass="107420" checksum="2784655A572A748C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0J2</accession>
    <name>A0AAU9F0J2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">RecQ-like DNA helicase BLM</fullName>
            <ecNumber evidence="17">5.6.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Bloom syndrome protein homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">DNA 3'-5' helicase BLM</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">RecQ helicase homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">DMAD_07864</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="17"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <sequence length="1325" mass="147987" checksum="570F23910604CDFF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <begin position="820"/>
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        <location>
            <begin position="1149"/>
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            <begin position="1287"/>
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        </location>
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        <location>
            <begin position="1359"/>
            <end position="1370"/>
        </location>
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            <begin position="1461"/>
            <end position="1482"/>
        </location>
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            <begin position="2055"/>
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            <begin position="2075"/>
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        <location>
            <begin position="2454"/>
            <end position="2464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF89044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2516" mass="275765" checksum="6523336276578580" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0L9</accession>
    <name>A0AAU9F0L9_DROMD</name>
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        <recommendedName>
            <fullName evidence="5">procollagen-proline 4-dioxygenase</fullName>
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        </recommendedName>
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        <name evidence="15" type="ORF">DMAD_09069</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
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    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15 16">RF1</strain>
        </source>
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        <text evidence="2">Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.</text>
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        <cofactor evidence="1">
            <name>L-ascorbate</name>
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        </cofactor>
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        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
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    </comment>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="1"/>
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        </location>
    </feature>
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            <begin position="17"/>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0M6</accession>
    <name>A0AAU9F0M6_DROMD</name>
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        <recommendedName>
            <fullName evidence="2">PID domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">DMAD_08459</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adapter_Engulfment-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033930">
        <property type="entry name" value="FAM43A/B_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
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        <property type="entry name" value="FI05246P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232">
        <property type="entry name" value="PHOSPHOTYROSINE INTERACTION DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14719">
        <property type="entry name" value="PID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="277"/>
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        </location>
    </feature>
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        </location>
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        </location>
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        <location>
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        </location>
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            <end position="676"/>
        </location>
    </feature>
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        <location>
            <begin position="677"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="870"/>
            <end position="880"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF89781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="880" mass="97565" checksum="5E0DDFC22B5669CF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0N0</accession>
    <name>A0AAU9F0N0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Julius seizure</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_10058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F0N0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="449"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="505"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF91860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="544" mass="59886" checksum="50D34F636CC824B0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0Q5</accession>
    <name>A0AAU9F0Q5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">BK channel</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Maxi K channel</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_10072</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. Slo sub-subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F0Q5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000035">
        <property type="entry name" value="Calcium-activated potassium channel slowpoke"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001832">
        <property type="entry name" value="Calcium-activated potassium channel slowpoke-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000015">
        <property type="entry name" value="Calcium-activated potassium channel subunit alpha-1 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01449">
        <property type="entry name" value="BKCHANNELA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="19">
        <location>
            <begin position="356"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="19">
        <location>
            <begin position="782"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="700"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1122"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="700"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF91890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1152" mass="127702" checksum="A44C08945E8A2446" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0R4</accession>
    <name>A0AAU9F0R4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Metalloendopeptidase</fullName>
            <ecNumber evidence="19">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_10076</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="18 19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="18 19">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="19"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008586">
        <property type="term" value="P:imaginal disc-derived wing vein morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032927">
        <property type="term" value="P:positive regulation of activin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030513">
        <property type="term" value="P:positive regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd04281">
        <property type="entry name" value="ZnMc_BMP1_TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000004">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000052">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000053">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000003">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000240">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015446">
        <property type="entry name" value="BMP_1/tolloid-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
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        <property type="entry name" value="Sperma_CUB_dom_sf"/>
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        <property type="entry name" value="ZnMP_TLD/BMP1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="3" id="KW-0964">Secreted</keyword>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F130</accession>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    <sequence length="458" mass="49517" checksum="0C12AA054EE13A31" modified="2024-11-27" version="1">MLQNPSKYHANPLSHFLALHNTQAAGGVGLGVGVGGGIPAHGQPHHALPTAALAAAAAAAAAVSAGQSSYQMEQQQHHHHHHQQHHQHSQQHHQQHQHQHQQQVQQQHQHQLPHPDSHHPPGTTGNNSSNNHREQLAAATATSHAQLFEAAAAHSSLDVGKSFTIAAILGLQSQGRKDYNNAINLSLNGAARDAIDQDNNHNNSNNNNNNNNNSQSVKNFNCDSSSGMSVGRYLPPHHPHHHPPGFAAAAAVAAAVGAAAPSALQSLQQLHQQHHAQQQATLSFQREKLKSDSHKKSSLKNKRVRTIFTPEQLECLEAEFERQQYMVGPERLYLAHTLKLTEAQVKVWFQNRRIKWRKHHLELTQQRLALIRQTQLPGTVMGPSSGSVGVSAGNSGSTDLTAGCSAASPSLQEEDNEDSKQSLSLPMPPLSRAQSESDLSICNDSLDGDSLMDGSEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F133</accession>
    <name>A0AAU9F133_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_08639</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F133"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043919536" evidence="1">
        <location>
            <begin position="25"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF90036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="174" mass="20151" checksum="1C26640D2138E067" modified="2024-11-27" version="1">MKVQHFFRVLMLAFLALSWSTSEARRHRIRVRHRHRHRISRFMRVKPPPMWPTCPAMERGPLVPLVKSAIMGQMSKKLLEKCGYEEELSQLEVWRETMHKKLDNDMVVDVIAWLYEEVEVLFNTPVVPQAGQAGHPAVSHPDSEGPTAPENCHDFRLYVQVVDDGVNVNDADWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F8Z6</accession>
    <name>A0AAU9F8Z6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine/threonine-protein kinase greatwall</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Microtubule-associated serine/threonine-protein kinase-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_10270</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F8Z6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000550">
        <property type="entry name" value="Serine/threonine-protein kinase greatwall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000278">
        <property type="entry name" value="serine/threonine-protein kinase greatwall isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000484">
        <property type="entry name" value="Serine/threonine-protein kinase greatwall, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="58"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="753"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="379"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="623"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="400"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="415"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF92145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="798" mass="88067" checksum="1058DB8B279042DE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F8Z7</accession>
    <name>A0AAU9F8Z7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">deoxyhypusine monooxygenase</fullName>
            <ecNumber evidence="4">1.14.99.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_10273</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[eIF5A protein]-deoxyhypusine + AH2 + O2 = [eIF5A protein]-hypusine + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:14101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="Rhea" id="RHEA-COMP:12592"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="ChEBI" id="CHEBI:91175"/>
            <dbReference type="EC" id="1.14.99.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; eIF5A hypusination.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.29" evidence="4"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F8Z7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019135">
        <property type="term" value="F:deoxyhypusine monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000099">
        <property type="entry name" value="Deoxyhypusine hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697:SF5">
        <property type="entry name" value="DEOXYHYPUSINE HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697">
        <property type="entry name" value="PBS LYASE HEAT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF92150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="238" mass="26548" checksum="001FF843F9F882A9" modified="2024-11-27" version="1">MQDPKALDILTKVLKDTTQEPMVRHEAAEAMGAIGHADVLAILEEYKQDPVVEVAETCAIALDRVRWLQSGQQVADNNPYASVDPSPPTAGDKSVAELKAIYLDAKQTLFDRYRAMFSLRNLRTEESVLAIAEGLKDSSALFRHEVAFVLGQLQEPCSIPYLQENLEDHKENEMVRHECAEALGAIASDECIQILNRYADDEKRVVKESCVIALDMCEYENSPEFQYADGLTKLDGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9C2</accession>
    <name>A0AAU9F9C2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphoribosyl pyrophosphate synthase-associated protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_10371</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F9C2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000031">
        <property type="entry name" value="Probable PRS4-ribose-phosphate pyrophosphokinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF53">
        <property type="entry name" value="GH23275P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="4">
        <location>
            <begin position="27"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="219"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="246"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF92282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="386" mass="42793" checksum="C809504BB0D0F66F" modified="2024-11-27" version="1">MSKRRLGSVRPPKRCRSDMDNTSTSDIVIINGNSHPDLANMVAERMGLKNGGCSVFHKSNRETIVEISDSVRGKDIYIIQTGTKDANNNIMELLIMAYACKTSSARSIVGVIPYLPYSKQCKMRKRGCIVSKLLAKMMCTSGLTHIITMDLHQKEIQGFFDIPVDNLRASPFLLQYIQESIPEYRNSVIVARNPGVAKKANSYAERLRLGLAVIHGEQKEAESDEVDGRYSPPPTRNSTPQHAPISSSSRQRTTSVSVGVPEHPVKVKPPLTIVGDVNGRIAIMVDDLIDDVQSFVAAAEMLKENGACKIYVLATHGLLSSDAPRLLDESCIDEIVVTNTIPHEIQKLQCHKIKTIDISILIAEAIRRIHNKESMSYLFRNVTLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9C3</accession>
    <name>A0AAU9F9C3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphoribosyl pyrophosphate synthase-associated protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_10371</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F9C3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000031">
        <property type="entry name" value="Probable PRS4-ribose-phosphate pyrophosphokinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF53">
        <property type="entry name" value="GH23275P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="4">
        <location>
            <begin position="9"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="201"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF92285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="368" mass="40682" checksum="EA01F33FB77CF46C" modified="2024-11-27" version="1">MDNTSTSDIVIINGNSHPDLANMVAERMGLKNGGCSVFHKSNRETIVEISDSVRGKDIYIIQTGTKDANNNIMELLIMAYACKTSSARSIVGVIPYLPYSKQCKMRKRGCIVSKLLAKMMCTSGLTHIITMDLHQKEIQGFFDIPVDNLRASPFLLQYIQESIPEYRNSVIVARNPGVAKKANSYAERLRLGLAVIHGEQKEAESDEVDGRYSPPPTRNSTPQHAPISSSSRQRTTSVSVGVPEHPVKVKPPLTIVGDVNGRIAIMVDDLIDDVQSFVAAAEMLKENGACKIYVLATHGLLSSDAPRLLDESCIDEIVVTNTIPHEIQKLQCHKIKTIDISILIAEAIRRIHNKESMSYLFRNVTLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9C7</accession>
    <name>A0AAU9F9C7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphoribosyl pyrophosphate synthase-associated protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_10371</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F9C7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000031">
        <property type="entry name" value="Probable PRS4-ribose-phosphate pyrophosphokinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF53">
        <property type="entry name" value="GH23275P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="4">
        <location>
            <begin position="9"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF92284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="387" mass="42601" checksum="DB2699E5A35B7917" modified="2024-11-27" version="1">MDNTSTSDIVIINGNSHPDLANMVAERMGLKNGGCSVFHKSNRETIVEISDSVRGKDIYIIQTGTKDANNNIMELLIMAYACKTSSARSIVGVIPYLPYSKQCKMRKRGCIVSKLLAKMMCTSGLTHIITMDLHQKEIQGFFDIPVDNLRASPFLLQYIQESIPEYRNSVIVARNPGVAKKANSYAERLRLGLAVIHGEQKEAESDEVDGRYSPPPTSAYSNLLGNSVEMCLPAAPRNSTPQHAPISSSSRQRTTSVSVGVPEHPVKVKPPLTIVGDVNGRIAIMVDDLIDDVQSFVAAAEMLKENGACKIYVLATHGLLSSDAPRLLDESCIDEIVVTNTIPHEIQKLQCHKIKTIDISILIAEAIRRIHNKESMSYLFRNVTLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAH0</accession>
    <name>A0AAU9FAH0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Suppressor of lurcher protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_10699</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FAH0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000108">
        <property type="entry name" value="SOL-1 related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053207">
        <property type="entry name" value="Non-NMDA_GluR_Accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47537">
        <property type="entry name" value="CUBILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47537:SF2">
        <property type="entry name" value="CUBILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="50"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF92696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="218" mass="25321" checksum="F1B95AA7461A823D" modified="2024-11-27" version="1">MSNGPQIHMQYFGKYPPEMTNNVQYYGFRAEYRFLTNFGIMSGVQQGEECTFVYNSSERISGLFHSPNFPGFYLENVVCNYYFYAGRDERVVVHFTYFDVEGIGACDHQTASDYVEFSNFMSTDRKYSKYCGKLQDFEMRSDGHFFRVTLHSNDRFVAIGFRALYTFEPIPTNNSNADLRGNASMQSYVSHAPSQQDFYNIIALILVAISFIKYNIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAL1</accession>
    <name>A0AAU9FAL1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA ligase</fullName>
            <ecNumber evidence="15">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_10700</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="15"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FAL1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070421">
        <property type="term" value="C:DNA ligase III-XRCC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07902">
        <property type="entry name" value="Adenylation_DNA_ligase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07967">
        <property type="entry name" value="OBF_DNA_ligase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000003">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF9">
        <property type="entry name" value="DNA LIGASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 15 20" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="360"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="19">
        <location>
            <begin position="714"/>
            <end position="804"/>
        </location>
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        <location>
            <begin position="630"/>
            <end position="679"/>
        </location>
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        <location>
            <begin position="630"/>
            <end position="640"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="RuleBase" id="RU004196"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF92698.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="809" mass="91475" checksum="2B14A4C0773B1590" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAM3</accession>
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        <submittedName>
            <fullName evidence="4">Collagen alpha chain2</fullName>
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        <name evidence="4" type="ORF">DMAD_10719</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BFF92729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9FAM3"/>
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        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050938">
        <property type="entry name" value="Collagen_Structural_Proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456:SF6">
        <property type="entry name" value="COLLAGEN ALPHA-1(XXIII) CHAIN-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456">
        <property type="entry name" value="SI:CH211-266K2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="5"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="813"/>
        </location>
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            <end position="167"/>
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        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
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            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
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        </location>
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            <end position="401"/>
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        <location>
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        </location>
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        <location>
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        </location>
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        <location>
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        </location>
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        <location>
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            <end position="591"/>
        </location>
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        <location>
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            <end position="644"/>
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        <location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="782"/>
            <end position="806"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF92729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="813" mass="83380" checksum="62DB11C55ACB6CE4" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAN1</accession>
    <name>A0AAU9FAN1_DROMD</name>
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        <submittedName>
            <fullName evidence="17">Probable cytochrome P450 9f2</fullName>
        </submittedName>
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        <name evidence="17" type="ORF">DMAD_10701</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
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        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the cytochrome P450 family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF92700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FAN1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:iron ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11056">
        <property type="entry name" value="CYP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000042">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF54">
        <property type="entry name" value="CYP9F3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043504884" evidence="16">
        <location>
            <begin position="23"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF92700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="517" mass="60097" checksum="F7C4776D12B1D8D7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAN2</accession>
    <name>A0AAU9FAN2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Histone acetyltransferase KAT6A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_10732</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FAN2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052253">
        <property type="entry name" value="CR1/CR2-DNA-binding_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031940">
        <property type="entry name" value="DUF4772"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13006:SF9">
        <property type="entry name" value="GLUCOSE TRANSPORTER 4 ENHANCER FACTOR, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13006">
        <property type="entry name" value="PAPILLOMAVIRUS REGULATORY FACTOR PRF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15997">
        <property type="entry name" value="DUF4772"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01366">
        <property type="entry name" value="c-clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DUF4772" evidence="10">
        <location>
            <begin position="5"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4772" evidence="10">
        <location>
            <begin position="124"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="234"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="302"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="423"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="485"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="648"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="423"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="447"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="531"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="592"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="648"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF15997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF92744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="760" mass="83895" checksum="1D19614F43B88CB0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAN3</accession>
    <name>A0AAU9FAN3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Saccharopine dehydrogenase-like oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_10698</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FAN3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000178">
        <property type="entry name" value="Saccharopine dehydrogenase-like oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051276">
        <property type="entry name" value="Saccharopine_DH-like_oxidrdct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286:SF5">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="3">
        <location>
            <begin position="9"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF92691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="426" mass="46569" checksum="8F58A057CAD031E0" modified="2024-11-27" version="1">MAAKKLDAIIFGATGFTGKYTVLEAVSVLKGLSWGIAGRNQEKLQSVLKEMSAKSKTDLSQTPIIIADVNNEASLLEMAQRCRIVVNTAGPYRFFGENVVKACIESGTHHVDVSGEPQYMESIQLKYNDLARERGVYVISACGFDSIPADLGVVFVEKNFDGVVNSVENFVHFGVKGGTKGTGSASLNTGTWESAIHAIANRSESIAIRRKLFPDRLPKFHPELKPRAPLSIANEVGKVILPFPEPDLPVVMRSQRYLYENEKKRPIQMQAYMLYPSRWVAGVVVFLAAVIGILAKFSLGRQLLLKYPSFFSGGMASRDGPSEERMERTFFRMTMKATGWPKSQKLVESSTPLSKTLTVQITGPNPAYGSACVALLSTAKTILNESDKMPSTGGVLPPGAAFSKTSLISELEKHEHGIKYEIVANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAN4</accession>
    <name>A0AAU9FAN4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-actin monooxygenase</fullName>
            <ecNumber evidence="4">1.14.13.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_10737</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-methionyl-[F-actin] + NADPH + O2 + H(+) = L-methionyl-(R)-S-oxide-[F-actin] + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:51308"/>
            <dbReference type="Rhea" id="RHEA-COMP:12953"/>
            <dbReference type="Rhea" id="RHEA-COMP:12956"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.225"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Mical family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.225" evidence="4"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120501">
        <property type="term" value="F:F-actin monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09439">
        <property type="entry name" value="LIM_Mical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000085">
        <property type="entry name" value="protein-methionine sulfoxide oxidase Mical isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000118">
        <property type="entry name" value="protein-methionine sulfoxide oxidase Mical isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000004">
        <property type="entry name" value="protein-methionine sulfoxide oxidase MICAL2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022735">
        <property type="entry name" value="bMERB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057494">
        <property type="entry name" value="Rossman_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF54">
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Hexapoda</taxon>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <property type="entry name" value="Tudor_FRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51641">
        <property type="entry name" value="AGENET_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Agenet-like" evidence="17">
        <location>
            <begin position="4"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Agenet-like" evidence="17">
        <location>
            <begin position="65"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="382"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="384"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="398"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="415"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="446"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="507"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="546"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="564"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="594"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="610"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="621"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="672"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFF92769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="698" mass="78031" checksum="CD353821921F7D6A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAN8</accession>
    <name>A0AAU9FAN8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Saccharopine dehydrogenase-like oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_10698</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FAN8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000178">
        <property type="entry name" value="Saccharopine dehydrogenase-like oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051276">
        <property type="entry name" value="Saccharopine_DH-like_oxidrdct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286:SF5">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="3">
        <location>
            <begin position="16"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF92693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="380" mass="41645" checksum="130149FCDAEFF283" modified="2024-11-27" version="1">MSAKSKTDLSQTPIIIADVNNEASLLEMAQRCRIVVNTAGPYRFFGENVVKACIESGTHHVDVSGEPQYMESIQLKYNDLARERGVYVISACGFDSIPADLGVVFVEKNFDGVVNSVENFVHFGVKGGTKGTGSASLNTGTWESAIHAIANRSESIAIRRKLFPDRLPKFHPELKPRAPLSIANEVGKVILPFPETDRSVVMRSQRYLYENEKKRPIQMQAYMLYPSRLVAGVVVFFAAVIGILAKFSFGRQLLLKYPSFFSGGMASRGGPSEARMERSFFRMTMKATGWPKSQKLAESTDQYSTLPSKTLTVQITGPNPGYGSTCVALLSTAKTILNESDKMPSTGGVLPPGAAFSKTSLISELEKHEHGIKYEIVANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAN9</accession>
    <name>A0AAU9FAN9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-actin monooxygenase</fullName>
            <ecNumber evidence="4">1.14.13.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_10737</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-methionyl-[F-actin] + NADPH + O2 + H(+) = L-methionyl-(R)-S-oxide-[F-actin] + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:51308"/>
            <dbReference type="Rhea" id="RHEA-COMP:12953"/>
            <dbReference type="Rhea" id="RHEA-COMP:12956"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.225"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Mical family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.225" evidence="4"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120501">
        <property type="term" value="F:F-actin monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09439">
        <property type="entry name" value="LIM_Mical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000085">
        <property type="entry name" value="protein-methionine sulfoxide oxidase Mical isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000118">
        <property type="entry name" value="protein-methionine sulfoxide oxidase Mical isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000004">
        <property type="entry name" value="protein-methionine sulfoxide oxidase MICAL2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022735">
        <property type="entry name" value="bMERB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057494">
        <property type="entry name" value="Rossman_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF54">
        <property type="entry name" value="[F-ACTIN]-MONOOXYGENASE MICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12130">
        <property type="entry name" value="bMERB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25413">
        <property type="entry name" value="Rossman_Mical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01203">
        <property type="entry name" value="DUF3585"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51848">
        <property type="entry name" value="BMERB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="P:inosine biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenosine deaminase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
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        <property type="entry name" value="ADGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01431">
        <property type="entry name" value="adm_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409:SF39">
        <property type="entry name" value="ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="A_deaminase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043672759" description="Adenosine deaminase" evidence="11">
        <location>
            <begin position="19"/>
            <end position="505"/>
        </location>
    </feature>
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        <location>
            <begin position="7"/>
            <end position="97"/>
        </location>
    </feature>
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        <location>
            <begin position="196"/>
            <end position="483"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00006083"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00012784"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00018099"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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            <dbReference type="ARBA" id="ARBA00047764"/>
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            <dbReference type="EMBL" id="BFF92875.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="505" mass="58007" checksum="5B3B6E9964CF9188" modified="2024-11-27" version="1">MKRISLLLLLSHLLSVQAQSQLSYEQARQAVLTAEEELMTGGHTYLSTQEAKADDSFMQYKLGELAQGFRNAEQNAAAMHFFKAKPLIDRSGVFRFLQKMPKGAVLHLHNSASVSSKWVVKNMSYMPGMLRCTTSTGRSVLTFRRTPKEHECQSGYVRVADERRSSIDPLVYDQNFERLINLYTPVPELEYPTITKVWDRFQSMFRTFGDAINYLPAFRAYHWQMLEELYNDNVMYAEIRSSFTPLYDASGRTFSRERTIQEFYALNEKFVRLHPDFLGIKLIYAVYRGHDVDKIGELVEDFRQLHKAFPNFVVGFDLVGQEDKGKPLYELLPALKDLPPTAHFFLHAGETNWFGASTDINLLDALLLNSTRIGHGYALAKHPILLNAVKTRQIAIEVSPISNQVLHLLWDMRNHPAAQYLALDVPLVICNDDPGFWNAKALSYDFYYAIMSLAPNNAGLRVLKTLVWNSVRYSLLTEEEQKRAYQILELTWSRFIDKVLQGSVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FB04</accession>
    <name>A0AAU9FB04_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">KANL2-like probable zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_10801</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Znf_KANL2-like"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16198:SF2">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13891">
        <property type="entry name" value="zf-C3HC3H_KANSL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1129"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="domain" description="KANL2-like probable zinc-finger" evidence="5">
        <location>
            <begin position="732"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="214"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="388"/>
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        </location>
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        </location>
    </feature>
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            <end position="856"/>
        </location>
    </feature>
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        <location>
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            <end position="913"/>
        </location>
    </feature>
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            <begin position="249"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
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            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
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            <end position="556"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="883"/>
            <end position="910"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF92828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1206" mass="127136" checksum="6BCD2B62884B2314" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FB06</accession>
    <name>A0AAU9FB06_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Papilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_10846</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the papilin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048019">
        <property type="term" value="F:receptor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050431">
        <property type="term" value="F:transforming growth factor beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd22639">
        <property type="entry name" value="Kunitz_papilin_lacunin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22637">
        <property type="entry name" value="Kunitz_papilin_mig6-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000020">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000107">
        <property type="entry name" value="Myosin, light chain kinase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000005">
        <property type="entry name" value="Pancreatic trypsin inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000017">
        <property type="entry name" value="papilin isoform X2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
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        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036645">
        <property type="entry name" value="Elafin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010909">
        <property type="entry name" value="PLAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008197">
        <property type="entry name" value="WAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45938">
        <property type="entry name" value="ACP24A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45938:SF11">
        <property type="entry name" value="WAP, KAZAL, IMMUNOGLOBULIN, KUNITZ AND NTR DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
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        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000035">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="7">
        <location>
            <begin position="84"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="8">
        <location>
            <begin position="389"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF95740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="568" mass="65006" checksum="1012AD239D734A3A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJC9</accession>
    <name>A0AAU9FJC9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinetochore protein NDC80</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_13292</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
            <location evidence="10">Centromere</location>
            <location evidence="10">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the NDC80/HEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJC9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.30">
        <property type="entry name" value="Ncd80 complex, Ncd80 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005550">
        <property type="entry name" value="Kinetochore_Ndc80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055260">
        <property type="entry name" value="Ndc80_CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038273">
        <property type="entry name" value="Ndc80_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03801">
        <property type="entry name" value="Ndc80_HEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 10" id="KW-0137">Centromere</keyword>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="4 10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Ndc80 CH" evidence="13">
        <location>
            <begin position="213"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="64"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="128"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="171"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007050"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF96011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="599" mass="69303" checksum="554C72A77B95B73C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJD1</accession>
    <name>A0AAU9FJD1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_13179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJD1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF95869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="94" mass="9946" checksum="DB9038F3FC58A0D4" modified="2024-11-27" version="1">MVAAVIPLGFFILISVIIVSISIMIAEVIVVMVRLIPVPMISEILWGRLLSTSAALAADGSIGIVVVEGKVHVVLLPEDAAPLQLDEGQQDQCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJD6</accession>
    <name>A0AAU9FJD6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DMAD_13085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJD6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF95752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="306" mass="34622" checksum="DD16A11E2FF1D0E6" modified="2024-11-27" version="1">MSKKDLQTIGVAGEAKSSSGVRCDEPSIMGRFGWAQFGNVCLPYIYRQTSTETEKYLSARMFELKLGTYLNYFQPDVYARCVYVPSYYITDAEARLFNEANDKHCDGQFGCDKFTRKDLVVRVGDAINFYQFLNDCYRKLLTGSNNSSDKFGFIRINKESVISYTVHNEERVLPLFHFRIETENLETKAVLLNGWDLAYLKFCCKVQGVGNEYYSGQNVAVMSLTYIKSAFPNGTEFEDYWPSKDVDTSLLIGNRANVNVNSVKWNTPTPLVRSAAGQNANHMSLAASATYQQQQRVAAMDSCNVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJD8</accession>
    <name>A0AAU9FJD8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_13035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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        </source>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRN1</accession>
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            <fullName evidence="9">E3 ubiquitin-protein ligase</fullName>
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        </recommendedName>
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        <name evidence="16" type="ORF">DMAD_06775</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="16 17">RF1</strain>
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        <reaction evidence="1 9">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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        <text evidence="3 9">Protein modification; protein ubiquitination.</text>
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        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-EC"/>
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            <position position="761"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50004"/>
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        <source>
            <dbReference type="PROSITE" id="PS50020"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50237"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF98681.1"/>
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    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
    <sequence length="794" mass="90630" checksum="6F954B108B0841D7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRN4</accession>
    <name>A0AAU9FRN4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tuberin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_06494</name>
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    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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</entry>
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        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF98230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="572" mass="64368" checksum="6D67902BAD93E4D3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRP3</accession>
    <name>A0AAU9FRP3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="13">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_06413</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="13"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRP3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003006">
        <property type="term" value="P:developmental process involved in reproduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000026">
        <property type="term" value="P:regulation of multicellular organismal development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007435">
        <property type="term" value="P:salivary gland morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05034">
        <property type="entry name" value="PTKc_Src_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09933">
        <property type="entry name" value="SH2_Src_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11845">
        <property type="entry name" value="SH3_Src_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000399">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000253">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000053">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000088">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 13" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="94"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="15">
        <location>
            <begin position="161"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="283"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFF98176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="551" mass="62873" checksum="D8416035EA397745" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRP4</accession>
    <name>A0AAU9FRP4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">pantothenate kinase</fullName>
            <ecNumber evidence="4">2.7.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_06780</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16373"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the type II pantothenate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.33" evidence="4"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRP4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="ASKHA_NBD_PanK-II_Pank1-like"/>
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    </dbReference>
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        <property type="entry name" value="Fbl, isoform B"/>
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    </dbReference>
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        <property type="entry name" value="pantothenate kinase 2, mitochondrial"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
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        <property type="entry name" value="Type_II_PanK"/>
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    <dbReference type="NCBIfam" id="TIGR00555">
        <property type="entry name" value="panK_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FUMBLE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PANTOTHENATE KINASE"/>
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    </dbReference>
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        <property type="entry name" value="Fumble"/>
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    </dbReference>
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        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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            <begin position="25"/>
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        </location>
    </feature>
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            <begin position="485"/>
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            <begin position="26"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00060870"/>
        </source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF98696.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="533" mass="58318" checksum="B610C58A2E8F7611" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRP9</accession>
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        <recommendedName>
            <fullName evidence="2">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">DMAD_06498</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">RF1</strain>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="PH-like_dom_sf"/>
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    </dbReference>
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        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 5"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13217">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 7"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DBL homology domain (DH-domain)"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="408"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="511"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="637"/>
            <end position="661"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="761"/>
            <end position="786"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1408"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="44"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
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    </feature>
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        <location>
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        <location>
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        <location>
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        <location>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="453"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="549"/>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1478"/>
            <end position="1492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF98310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1492" mass="161578" checksum="BD80CC9A3600A106" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRT8</accession>
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        <submittedName>
            <fullName evidence="5">Protein FAM135A</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="ORF">DMAD_06546</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM135 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
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    </dbReference>
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        <property type="entry name" value="Protein FAM135A isoform a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
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        <property type="entry name" value="DUF3657"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF5">
        <property type="entry name" value="DUF676 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12394">
        <property type="entry name" value="DUF3657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1208"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="48"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="454"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="640"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="744"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="863"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="921"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1089"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1121"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="304"/>
            <end position="331"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Diptera</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="17 18">RF1</strain>
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        <text evidence="3">Belongs to the FNBP1 family.</text>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="612" mass="68746" checksum="F0278C5EA77E1D3D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FS11</accession>
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        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="13" type="ORF">DMAD_06842</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13 14">RF1</strain>
        </source>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="Znf_C2H2_sf"/>
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        <property type="entry name" value="Znf_C2H2_type"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="9"/>
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    <dbReference type="SUPFAM" id="SSF57667">
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        <property type="match status" value="5"/>
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    </dbReference>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF98165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2159" mass="239585" checksum="DE6A120505D99B69" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3M1</accession>
    <name>A0AAU9G3M1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">methylated diphthine methylhydrolase</fullName>
            <ecNumber evidence="6">3.1.1.97</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_01727</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>diphthine methyl ester-[translation elongation factor 2] + H2O = diphthine-[translation elongation factor 2] + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42656"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="3.1.1.97"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DPH7 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.97" evidence="6"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3M1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061685">
        <property type="term" value="F:diphthine methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052415">
        <property type="entry name" value="Diphthine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042:SF1">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFG02139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="364" mass="40711" checksum="1941D008E91475A3" modified="2024-11-27" version="1">MFKTLHSVDTEYSADSVEWCAQDDQHAEYFACGTYQLVQLEQGEEEDDETTKKRPRKGRVYLYHFETGSGALERLQNIETAAILDMKWLPAWSSDSGPLLATANALGEVEIYELLADEKQLQKRTCLALTAAAATAAAEAPLALALDWQRDGDAVQLVVSDSKGNISHLRYTAQGELSVVGTWNAHGFEAWTCTFDRWTPQRVYSGGDDCLLLAHDLRTEQRVWTNRAHQAGVTCLLSHPQHEHHLLTGSYDEQLRLFDTRAMKRTLAEVNLGGGIWRLKPHPKRLDVILAACMYTNFSVVQLDLKDSALTLLGTYEEHSSICYGADWHPSLSEDDGLTHMATCSFYDHKMCVSSVHLGCVKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3M7</accession>
    <name>A0AAU9G3M7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Protein sidekick</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_01876</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the sidekick family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002352">
        <property type="entry name" value="Blast:Protein sidekick"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000028">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000677">
        <property type="entry name" value="protein sidekick isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001469">
        <property type="entry name" value="protein sidekick isoform X9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000158">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000209">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000231">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001132">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001362">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001432">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001549">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001715">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001722">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001849">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002022">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF46">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="2031"/>
            <end position="2053"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="89"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="278"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="376"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="472"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="563"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="659"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="780"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="882"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="993"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="1091"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="1191"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="1297"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="1398"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
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                <person name="Yamamoto D."/>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
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                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13 14">RF1</strain>
        </source>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="protein sequence ID" value="BFG02759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-0813">Transport</keyword>
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    </feature>
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    </feature>
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        </location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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            <end position="847"/>
        </location>
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        <location>
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        </location>
    </feature>
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        </location>
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        <source>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG02759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="902" mass="97915" checksum="82A406329E3EE0ED" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3N5</accession>
    <name>A0AAU9G3N5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Potassium voltage-gated channel protein Shaker</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_02143</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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            <fullName evidence="9">Probable serine/threonine-protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_02044</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9 10">RF1</strain>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the REXO1/REXO3 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:exonuclease activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06145">
        <property type="entry name" value="REX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000031">
        <property type="entry name" value="RNA exonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034922">
        <property type="entry name" value="REX1-like_exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF115">
        <property type="entry name" value="FI18136P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Exonuclease" evidence="8">
        <location>
            <begin position="669"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="22"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="168"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="518"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="28"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
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        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="226"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="266"/>
            <end position="319"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="320"/>
            <end position="330"/>
        </location>
    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="384"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
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            <begin position="522"/>
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    </feature>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00006357"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG02565.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="838" mass="93210" checksum="D89004AD506BD9A5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3U3</accession>
    <name>A0AAU9G3U3_DROMD</name>
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        <submittedName>
            <fullName evidence="20">Potassium voltage-gated channel protein eag</fullName>
        </submittedName>
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        <name evidence="20" type="ORF">DMAD_02225</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome A"/>
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        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005249">
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0042391">
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Jelly Rolls"/>
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    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="348"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="420"/>
            <end position="442"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="449"/>
            <end position="469"/>
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        <location>
            <begin position="43"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="19">
        <location>
            <begin position="94"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="17">
        <location>
            <begin position="575"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="718"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="767"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="817"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="860"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="960"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1057"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1126"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1210"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="739"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="772"/>
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        </location>
    </feature>
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        <location>
            <begin position="817"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="1057"/>
            <end position="1069"/>
        </location>
    </feature>
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        <location>
            <begin position="1070"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="1138"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1153"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1221"/>
            <end position="1239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFG02819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1265" mass="135094" checksum="3331D80737CFA0F8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3U4</accession>
    <name>A0AAU9G3U4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">NADH dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_02502</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3U4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.600:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11540:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050837">
        <property type="entry name" value="ComplexI_51kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054765">
        <property type="entry name" value="SLBB_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010120">
        <property type="entry name" value="PRK13596.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE FLAVOPROTEIN 1 NDUFV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22461">
        <property type="entry name" value="SLBB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="Nqo1 FMN-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142984">
        <property type="entry name" value="Nqo1 middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="Nqo1C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0288">FMN</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 51kDa subunit iron-sulphur binding" evidence="10">
        <location>
            <begin position="287"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG03190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="380" mass="42053" checksum="42BA95D249ACAA5B" modified="2024-11-27" version="1">MDQVCQSGLRGRGGGGYFSGTKWNFLRTAEANADKVLVMNCAEGEPGTCKDREILRHEPHKLIEGCLLAGFAMGCTRAIIYVRNRFYNEACNLQFALAEAYRHGLLGSSACSTGIRFDILVQRGDRYLCGEETALVKCLMGEIGRPRRRPPFLTEKGYFSHPCLVINAESVAVLPTILRRGPRWWADLGRSYNSGTKLFNISGHVNCPCTVEEEMSMPMRDLIEKHAGGVRGGWDNLLAVFPGGLSTSLINKQVAAEVLMDFECLEAAGSSLGSGALIVVNKQSDPIKVMLRSIQFYEKHTCKQCTYCRDGAIWLPELFDRFVRGEAHPHEIDWTVAIGKKIKDSNPICGLAQGQVNVACSLVDNFRPQIEDRILNCIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3U5</accession>
    <name>A0AAU9G3U5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Protein sidekick</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_01876</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the sidekick family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002352">
        <property type="entry name" value="Blast:Protein sidekick"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000028">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000677">
        <property type="entry name" value="protein sidekick isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001469">
        <property type="entry name" value="protein sidekick isoform X9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000158">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000209">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000231">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001132">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001362">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001432">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001549">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001715">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001722">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001849">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002022">
        <property type="entry name" value="Sidekick, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF46">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG02824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G402</accession>
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        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="17" type="ORF">DMAD_01829</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF23">
        <property type="entry name" value="TOUSLED-LIKE KINASE, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="955"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
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            <end position="80"/>
        </location>
    </feature>
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            <end position="160"/>
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    </feature>
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            <end position="278"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
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            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
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            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
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            <end position="549"/>
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    </feature>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
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            <end position="880"/>
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    </feature>
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        <location>
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            <end position="1294"/>
        </location>
    </feature>
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        <location>
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            <end position="940"/>
        </location>
    </feature>
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        <location>
            <begin position="23"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="98"/>
            <end position="143"/>
        </location>
    </feature>
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        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
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        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
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            <end position="397"/>
        </location>
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        </location>
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        </location>
    </feature>
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        </location>
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    </feature>
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            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
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            <end position="870"/>
        </location>
    </feature>
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        <location>
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            <end position="1266"/>
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        <location>
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            <end position="1294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="984"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG02274.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G407</accession>
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            <shortName evidence="5">Secretory carrier membrane protein</shortName>
        </recommendedName>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
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        <property type="project" value="InterPro"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="240"/>
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        <location>
            <begin position="260"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG02446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="339" mass="37895" checksum="2728AB68F943B2FC" modified="2024-11-27" version="1">MSGSGLDENPFGEPNLDNPFADPAIQQARRQAGAALVSLEDYNPFEEQAKPQLQINSTNTAAVVQPLSQNVPPPQTSSLGASAPSTSIQITSEELQEELDRKAAELDRREQQLQGNVPQLNNWPPLPDNFCVKPCFYQDFEVEIPPEFQRLVKHLYYIWMFYTMTLVANVIGGLILLFHSGDFVTLFLALFYTVLFTPASYVCWFRPAYKAFRNDSSFNFMVFFFIYFFQTIYSVVQAVGFDKMGYCGFITAISQFDGQAAGIIVGILLLNVAFCFTAVAVANILMITKIHTIYRSTGASMAKAQAEFTTEFLRNQHVQEAASSAVNTAISSQFNNSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G408</accession>
    <name>A0AAU9G408_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">WSCD family member</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_01941</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WSCD family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G408"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051589">
        <property type="entry name" value="Sialate-O-sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964">
        <property type="entry name" value="WSCD FAMILY MEMBER CG9164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964:SF5">
        <property type="entry name" value="WSCD FAMILY MEMBER CG9164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13469">
        <property type="entry name" value="Sulfotransfer_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG02442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="316" mass="36445" checksum="5DF57A96331EDC95" modified="2024-11-27" version="1">MALQGWRFFGVSATIIIYIGGVLFLSMNNIPGSHPKRPRIERFAEFPSFHSPRFPMPSRKMTIRWCRDLKYMNRDLPNYTDYRADFYTLPSDVSASLQASPMLTALASFPGSGNTWLRYLLQQATGILTGSIYKDYGLLKTGFPAENVCNSSVLLVKTHEWGGKAWAPFAKAILLVRDPEKAIIAEFNRQSGGHIGFASPDRYKRTKGKYWQQFVSNKLKGWELMNLSWARNFTGSIKVVFYDDLVHHTERELRSILDFLQFPVDETLLRCAILRKEGIFRRKKRLLSFDPYTEAMRAEVQARKRIVYGLLGRKEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G412</accession>
    <name>A0AAU9G412_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Dopamine D2-like receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_02117</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G412"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001591">
        <property type="term" value="F:dopamine neurotransmitter receptor activity, coupled via Gi/Go"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15053">
        <property type="entry name" value="7tmA_D2-like_dopamine_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000233">
        <property type="entry name" value="CLUMA_CG012980, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000247">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF125">
        <property type="entry name" value="DOPAMINE D2-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="384"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="419"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="539"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="812"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="851"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="399"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="604"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="702"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="764"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="8"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="716"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="728"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG02668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="890" mass="93600" checksum="D0A3E1BFAF89C0F3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G457</accession>
    <name>A0AAU9G457_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TRPL translocation defect protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_02544</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G457"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070300">
        <property type="term" value="F:phosphatidic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045494">
        <property type="term" value="P:photoreceptor cell maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001321">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.320.10:FF:000003">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CYTH-like_dom_sf"/>
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        <property type="entry name" value="NadR/Ttd14_AAA_dom"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="TRPL TRANSLOCATION DEFECT PROTEIN 14"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NadR/Ttd14 AAA" evidence="2">
        <location>
            <begin position="86"/>
            <end position="268"/>
        </location>
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            <end position="71"/>
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            <begin position="57"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
    <sequence length="489" mass="54718" checksum="203F8D4F60D9B4B3" modified="2024-11-27" version="1">MATAADQLMSPAALATIATVSNGQQSLLATTALAPSAAAAMAVRNGQEPQELVESQKSTSPKPSPMPSLKLNKMGSVSSKEKRVYKIVLTGGPCGGKTTGQSRLCTFFENLGWKVFRVPETATVLLSGGVKFSDLTEKEAYKFQENLIRTMVQIENTYFELGNSSTRNCLIICDRGVMDASAYISKDKWEKMMAGNKWNPVEMRDNRYNQILHLVSAANGAEDFYSTEDHACRSEGVDLARELDYKSAAAWVGHPYFDVIDNSTNFEAKMNRLIESVCQKVGIDIGDRLQATSRKLKYLVAMLPPDSEFPPFQDFDVVHHYLQSGGPKVQARLRKRGQKSHWSYIHTQRRPNVHGQARIEVKTQLTHRDYMNLLAQRDDAHFTIYKKRRCFLINNQYFQLDIYKEPGHPRCKGLVLLETYSSLTGDALKNCMPKFLNIVKEVTGDPDYSMFNLSLKEDWSTTKKFCRTATHAKGAANGVANTPLVLNGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G461</accession>
    <name>A0AAU9G461_DROMD</name>
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        <submittedName>
            <fullName evidence="16">Protein sidekick</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_01876</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the sidekick family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFG02345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
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    </dbReference>
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        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Blast:Protein sidekick"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000028">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
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        <property type="entry name" value="protein sidekick isoform X1"/>
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    </dbReference>
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        <property type="entry name" value="protein sidekick isoform X9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="19"/>
    </dbReference>
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        <property type="entry name" value="FN3_dom"/>
    </dbReference>
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        <property type="entry name" value="FN3_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF46">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
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    </dbReference>
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        <property type="match status" value="13"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="2025"/>
            <end position="2047"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="89"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="278"/>
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        </location>
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    <feature type="domain" description="Ig-like" evidence="14">
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="563"/>
            <end position="652"/>
        </location>
    </feature>
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        <location>
            <begin position="659"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="774"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="876"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="987"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="1085"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="1185"/>
            <end position="1286"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="1291"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="1392"/>
            <end position="1485"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="1490"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="1591"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="1698"/>
            <end position="1801"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="1805"/>
            <end position="1897"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="1903"/>
            <end position="2007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2091"/>
            <end position="2181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="2096"/>
            <end position="2108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="2136"/>
            <end position="2146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="2168"/>
            <end position="2181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG02345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2248" mass="249527" checksum="CB4BE4F9661E4FAC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G465</accession>
    <name>A0AAU9G465_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Insulin-like growth factor 2 mRNA-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_02129</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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</entry>
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        </source>
    </evidence>
    <sequence length="310" mass="34385" checksum="DC1F1E1B8BFF0A0C" modified="2024-11-27" version="1">MVLVLNGVLQDDCPMDTNSLFLQHPVYRDYAQQLHSIQAKSVGPVGLLYVGQRELAASAPHDKNVNIIGADDATTCIIVVVRHSGSGAVALAHFDGSGVDEAVCTMVSRVQELALGYPEGRIELQLIGGYRDSKGYGEDVFYSIMQSFHNHHLEIDLTQACVGELNTMLRGEINCPIIYGVGVNIKTGEIFPASFPDRGPDRELRDARIFMGAQSVLDVYDSTLGMLRIGPFNYDPLRGADLWLSQTDEFLLQHLSSSPDVEPPHFAPQTRATIKFIQENQFPAVTVFRDNRPRYFRRDDATGCWALIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDQ6</accession>
    <name>A0AAU9GDQ6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein lin-54 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_04654</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDQ6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="5">
        <location>
            <begin position="755"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="342"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="469"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="520"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="679"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="873"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="352"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="399"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="469"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="521"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="679"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="873"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="884"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFG06060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="967" mass="101749" checksum="996B9B1D6F98BFE4" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDQ8</accession>
    <name>A0AAU9GDQ8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Superoxide dismutase [Cu-Zn]</fullName>
            <ecNumber evidence="9">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_04721</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="9">Binds 1 copper ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="9"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDQ8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00305">
        <property type="entry name" value="Cu-Zn_Superoxide_Dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000003">
        <property type="entry name" value="Superoxide dismutase [Cu-Zn], chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018152">
        <property type="entry name" value="SOD_Cu/Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00068">
        <property type="entry name" value="CUZNDISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00087">
        <property type="entry name" value="SOD_CU_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00332">
        <property type="entry name" value="SOD_CU_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6 9" id="KW-0186">Copper</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043605702" description="Superoxide dismutase [Cu-Zn]" evidence="10">
        <location>
            <begin position="19"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="11">
        <location>
            <begin position="43"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG06138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="216" mass="22528" checksum="1BD5BDC45F872649" modified="2024-11-27" version="1">MMQSIVISLALCATICAAAQTRNTPIQAIAYVSGPVQADGSQVKGNVTFTQNDCGQNVHVRVQLEGLKEGKHGFHVHEKGDLSNGCASTGGHYNPDKVDHGGPDHEVRHVGDLGNLEVNSTGVIDITYTDKVISLTGNRGIIGRAVVVHELEDDLGLGDHVDSKKTGNAGGRIGCAVIGVNSDVDEWPCRDGGAGGLRSSLSILTVVVALFLARSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDR0</accession>
    <name>A0AAU9GDR0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14114">
        <property type="entry name" value="STKc_Twitchin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000321">
        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000440">
        <property type="entry name" value="Bent, isoform C"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDR1</accession>
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        <recommendedName>
            <fullName evidence="11">Semaphorin-2A</fullName>
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        <name evidence="15" type="ORF">DMAD_04387</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15 16">RF1</strain>
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        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="entry name" value="SEMAPHORIN 2B, ISOFORM D-RELATED"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG05712.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="760" mass="86637" checksum="D8F4E484CBC85B3B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDU1</accession>
    <name>A0AAU9GDU1_DROMD</name>
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        <recommendedName>
            <fullName evidence="6">PHD-type domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="7" type="ORF">DMAD_04508</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
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    </dbReference>
    <dbReference type="InterPro" id="IPR052440">
        <property type="entry name" value="Trans_Reg/Chrom_Remod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14955:SF4">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14955">
        <property type="entry name" value="RETINOIC ACID INDUCED 1/TRANSCRIPTION FACTOR 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDV0</accession>
    <name>A0AAU9GDV0_DROMD</name>
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        <recommendedName>
            <fullName evidence="20">Very long-chain fatty acid transport protein</fullName>
            <ecNumber evidence="14">6.2.1.3</ecNumber>
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            <fullName evidence="16">Long-chain-fatty-acid--CoA ligase</fullName>
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        <alternativeName>
            <fullName evidence="21">Very-long-chain acyl-CoA synthetase</fullName>
        </alternativeName>
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        <name evidence="25" type="ORF">DMAD_04347</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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    <reference evidence="25 26" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="25 26">RF1</strain>
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    <comment type="function">
        <text evidence="19">Acyl-CoA synthetase required for both the import of long chain fatty acids (LCFAs) (C14-C18) and the activation very long chain fatty acids (VLCFAs) (C20-C26) by esterification of the fatty acids into metabolically active CoA-thioesters for subsequent degradation or incorporation into phospholipids. The transport and fatty acyl-CoA synthetase activities are genetically separable and are thus independent activities. Esterifies VLCFAs in the peroxisome matrix. The VLCFAs are actively transported into peroxisomes by a PXA1-PXA2 heterodimeric transporter in the peroxisomal membrane.</text>
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        <reaction evidence="15">
            <text>a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
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            <dbReference type="Rhea" id="RHEA:54537"/>
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    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>tetracosanoate + ATP + CoA = tetracosanoyl-CoA + AMP + diphosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
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        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:33640"/>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <subcellularLocation>
            <location evidence="17">Peroxisome membrane</location>
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    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
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        <property type="entry name" value="PRK08279.1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FATTY ACID TRANSPORT PROTEIN 3, ISOFORM A"/>
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        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
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        <property type="entry name" value="AMP_BINDING"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="5" id="KW-0436">Ligase</keyword>
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    <keyword evidence="11" id="KW-0445">Lipid transport</keyword>
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    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDV5</accession>
    <name>A0AAU9GDV5_DROMD</name>
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        <submittedName>
            <fullName evidence="13">Microtubule-associated protein RP/EB family member 1</fullName>
        </submittedName>
    </protein>
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        <name evidence="13" type="ORF">DMAD_04765</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        </lineage>
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    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
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        <text evidence="2">Belongs to the MAPRE family.</text>
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        <property type="protein sequence ID" value="BFG06198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:microtubule binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="14"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="12">
        <location>
            <begin position="216"/>
            <end position="287"/>
        </location>
    </feature>
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        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="BFG06198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="298" mass="33463" checksum="73BA3AAE2A4244DC" modified="2024-11-27" version="1">MAVNVYSTNVTSENLSRHDMLAWVNDCLQSQFSKIEELCTGAAYCQFMDMLFPNSVPVKRVKFRTNLEHEYIQNFKILQAGFKKMSVDKIIPVDKLVKGRFQDNFEFLQWFKKFFDANYDGREYEPLAQRGGVNMGSGKGPGGSSNGGSGVGSVHNDIHTMHRRGILAPQPKRLAPSVPAHEPVSKVLPRTSSAVTHSRVATATNITGTVKKNDANHAVNTQQIDELSNQVMDMRLNLEGLEKERDFYFSKLRDIEILCQEAEEGETTPLVQKILDILYATEDGFAPPDDAPPEEEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDV7</accession>
    <name>A0AAU9GDV7_DROMD</name>
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        <submittedName>
            <fullName evidence="7">Protein Gawky</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_13653</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GW182 family.</text>
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    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR041971">
        <property type="entry name" value="Gawky_UBA"/>
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    <dbReference type="InterPro" id="IPR052068">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROTEIN GAWKY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRINUCLEOTIDE REPEAT-CONTAINING GENE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ago_hook"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00076">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="UBA-like"/>
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    </dbReference>
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        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="UBA" evidence="6">
        <location>
            <begin position="574"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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        </location>
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        </location>
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        </location>
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        </location>
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        </location>
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        </location>
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        <location>
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        </location>
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        <location>
            <begin position="1248"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="492"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="517"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="860"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="946"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="1112"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1248"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1284"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1295"/>
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        </location>
    </feature>
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        <location>
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        <location>
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        </location>
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        <location>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1384"/>
            <end position="1399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007302"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG06737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1419" mass="146014" checksum="A01F722E469FA89E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDV9</accession>
    <name>A0AAU9GDV9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mucin-5AC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_04448</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="930" mass="100616" checksum="1BAFD637CD80E2B5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GE25</accession>
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        <submittedName>
            <fullName evidence="19">Potassium voltage-gated channel subfamily H member 8</fullName>
        </submittedName>
    </protein>
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        <name evidence="19" type="ORF">DMAD_04522</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="BFG05896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000145">
        <property type="entry name" value="Eag-like K[+] channel, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000097">
        <property type="entry name" value="Eag-like K[+] channel, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000001">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.260:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003950">
        <property type="entry name" value="K_chnl_volt-dep_ELK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF637">
        <property type="entry name" value="EAG-LIKE K[+] CHANNEL, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
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        <property type="entry name" value="ELKCHANNEL"/>
    </dbReference>
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        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="479"/>
            <end position="503"/>
        </location>
    </feature>
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        <location>
            <begin position="35"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="18">
        <location>
            <begin position="92"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="16">
        <location>
            <begin position="581"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
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            <begin position="725"/>
            <end position="849"/>
        </location>
    </feature>
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            <end position="989"/>
        </location>
    </feature>
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            <end position="1079"/>
        </location>
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        </location>
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        <location>
            <begin position="1189"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="742"/>
            <end position="754"/>
        </location>
    </feature>
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        <location>
            <begin position="768"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="796"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="1063"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="1116"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1140"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="1217"/>
            <end position="1227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG05896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1305" mass="142999" checksum="3713ACEA25F30B84" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GE27</accession>
    <name>A0AAU9GE27_DROMD</name>
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        <recommendedName>
            <fullName evidence="3">Wurstfest</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DMAD_04779</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
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            <dbReference type="EMBL" id="BFG06223.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="54303" checksum="4C142568520C240B" modified="2024-11-27" version="1">MVNESKPTVPKPSTKEVECQSSDCENSCSMPEQQLVTSPKGGRVGIFNAEDFLSRAQMNDKINNILRSPTKPANGVRQRSVYTNNPSPASMRIGMTGGQLSQRVSNLSLSSGGCRMEQLGSGSGSSSSLTGSASVGTNTIGNGLVNDPAFAYQSGSQSARQYVHLPGTHLPKQPQYSAGNLQGMEAAGNLSRSGKALTARYHTPSSYGFEGEQMYGQAKGQLAPPMPPPPYALGRWSSTRLFEFENNTPSPCPGSGPMAMSNGTIQLRLRDGVRIDMTLDKAVRVLNQRSMVAVALSRNTSNSAMIHPNGRILQSGSKVEIVTYDGMKGNNFVRYAKMWYKGVSFTSESCALIYMVDTAGTRTTTDTFTDLTKDYTLAVFYDDSRHGPSYVAEAHEVIANASYSCAEDGTEIYDINGFRITQAADGLVKVTRVYNKCLIRTSPSNGSATLTTPGIHCTASLGKTSHLFVRRNEKRMHFDGSCFIVRNAGHSAGFNENNLLIVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GE37</accession>
    <name>A0AAU9GE37_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Metabotropic glutamate receptor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_04594</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF89726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="13" mass="1524" checksum="5E558F8E1A85B403" modified="2024-11-27" version="1" fragment="single">TLKCLKRTMLGYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXA3</accession>
    <name>A0AAU9EXA3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst complex component Sec8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_09854</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the SEC8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032584">
        <property type="term" value="C:growth cone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039682">
        <property type="entry name" value="Sec8/EXOC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007191">
        <property type="entry name" value="Sec8_exocyst_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04048">
        <property type="entry name" value="Sec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec8 N-terminal" evidence="8">
        <location>
            <begin position="426"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="L27" evidence="7">
        <location>
            <begin position="527"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="806"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1041"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="809"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF91616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1360" mass="152983" checksum="8D38EE3C62E622F5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EXA7</accession>
    <name>A0AAU9EXA7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst complex component Sec8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_09854</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the SEC8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032584">
        <property type="term" value="C:growth cone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039682">
        <property type="entry name" value="Sec8/EXOC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007191">
        <property type="entry name" value="Sec8_exocyst_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04048">
        <property type="entry name" value="Sec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec8 N-terminal" evidence="8">
        <location>
            <begin position="423"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="L27" evidence="7">
        <location>
            <begin position="524"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="803"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1038"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="806"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF91618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1357" mass="152623" checksum="A062E2A8BFA4274E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0N7</accession>
    <name>A0AAU9F0N7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">PRKR-like endoplasmic reticulum kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_08470</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F0N7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004694">
        <property type="term" value="F:eukaryotic translation initiation factor 2alpha kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
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    <keyword evidence="9 23" id="KW-0418">Kinase</keyword>
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    <keyword evidence="7" id="KW-0732">Signal</keyword>
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    <keyword evidence="5" id="KW-0808">Transferase</keyword>
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    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
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        <source>
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    <sequence length="1155" mass="129192" checksum="82A991B2CBEFC572" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <reaction evidence="13">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
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    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <begin position="101"/>
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        <location>
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        <location>
            <begin position="3874"/>
            <end position="3886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="3898"/>
            <end position="3920"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF91875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="3920" mass="441792" checksum="BE23FAED81A83BD6" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F0P8</accession>
    <name>A0AAU9F0P8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Inositol 1,4,5-trisphosphate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_08481</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Endoplasmic reticulum membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="16">The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the InsP3 receptor family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF89811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005220">
        <property type="term" value="F:inositol 1,4,5-trisphosphate-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23277">
        <property type="entry name" value="beta-trefoil_MIR_ITPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000002">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.30:FF:000001">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor, type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.30">
        <property type="entry name" value="IP3 receptor type 1 binding core, RIH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000493">
        <property type="entry name" value="InsP3_rcpt"/>
    </dbReference>
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        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
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        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013662">
        <property type="entry name" value="RIH_assoc-dom"/>
    </dbReference>
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        <property type="entry name" value="RIH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035910">
        <property type="entry name" value="RyR/IP3R_RIH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816">
        <property type="entry name" value="MIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816:SF4">
        <property type="entry name" value="RYR_IP3R HOMOLOGY ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08454">
        <property type="entry name" value="RIH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RYDR_ITPR"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="INSP3RECEPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100909">
        <property type="entry name" value="IP3 receptor type 1 binding core, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 16" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4 16" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="8 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15 16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14 16" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 16 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="2366"/>
            <end position="2398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="2410"/>
            <end position="2429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="2450"/>
            <end position="2474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="2494"/>
            <end position="2522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="2543"/>
            <end position="2565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="2683"/>
            <end position="2706"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="18">
        <location>
            <begin position="115"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="18">
        <location>
            <begin position="237"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1073"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1082"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU368044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFF89811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2859" mass="321619" checksum="ECB5D3A05F327A00" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F111</accession>
    <name>A0AAU9F111_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_09276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F111"/>
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    </dbReference>
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        <property type="entry name" value="FI19613P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21219:SF4">
        <property type="entry name" value="PID DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="615"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="659"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="403"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="616"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="660"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="696"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF90824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1060" mass="113489" checksum="65F3344C4E6BE7D8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F3Y2</accession>
    <name>A0AAU9F3Y2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Tyrosine-protein phosphatase 99A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_09618</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein-tyrosine phosphatase family. Receptor class subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F3Y2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14550">
        <property type="entry name" value="R5-PTP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14549">
        <property type="entry name" value="R5-PTPc-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001386">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000068">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001528">
        <property type="entry name" value="Tyrosine-protein phosphatase 99A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000097">
        <property type="entry name" value="Tyrosine-protein phosphatase 99A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF540">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 99A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043583256" evidence="13">
        <location>
            <begin position="29"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="387"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="169"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="266"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="14">
        <location>
            <begin position="472"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="15">
        <location>
            <begin position="654"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="14">
        <location>
            <begin position="760"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="33"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="89"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1052"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="104"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1054"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1066"/>
            <end position="1108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF91309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1215" mass="135964" checksum="8081DA601A6301B0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F3Y5</accession>
    <name>A0AAU9F3Y5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">BK channel</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Maxi K channel</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_10072</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. Slo sub-subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F3Y5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000035">
        <property type="entry name" value="Calcium-activated potassium channel slowpoke"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000005">
        <property type="entry name" value="calcium-activated potassium channel subunit alpha-1 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000015">
        <property type="entry name" value="Calcium-activated potassium channel subunit alpha-1 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01449">
        <property type="entry name" value="BKCHANNELA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16 23" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="8" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="22">
        <location>
            <begin position="339"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="22">
        <location>
            <begin position="813"/>
            <end position="957"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="664"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="729"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1153"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="729"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00060897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BFF91896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1183" mass="131511" checksum="CBF77EEB6E020A95" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F4A4</accession>
    <name>A0AAU9F4A4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamine--fructose-6-phosphate transaminase (isomerizing)</fullName>
            <ecNumber evidence="3">2.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_07791</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="3"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F4A4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000001">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 11" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="9">
        <location>
            <begin position="2"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="360"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="531"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="220"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF88906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="682" mass="76512" checksum="84BA09FB2B4585B0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9B2</accession>
    <name>A0AAU9F9B2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.143</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Beta-1,2-N-acetylglucosaminyltransferase II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">GlcNAc-T II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Mannoside acetylglucosaminyltransferase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">DMAD_10342</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>an N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12941"/>
            <dbReference type="Rhea" id="RHEA-COMP:13526"/>
            <dbReference type="Rhea" id="RHEA-COMP:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:60615"/>
            <dbReference type="ChEBI" id="CHEBI:60651"/>
            <dbReference type="EC" id="2.4.1.143"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 24">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 16 (GT16) protein family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.143" evidence="5"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F9B2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008455">
        <property type="term" value="F:alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009312">
        <property type="term" value="P:oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007754">
        <property type="entry name" value="GlcNAc_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12871:SF0">
        <property type="entry name" value="ALPHA-1,6-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12871">
        <property type="entry name" value="BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05060">
        <property type="entry name" value="MGAT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17 24" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 26" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="160"/>
            <end position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="266"/>
            <end position="270"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="233"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="320"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="371"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="376"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014817"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00029663"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00032552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032915"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00093257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-3"/>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="BFF92247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="488" mass="56271" checksum="55F1B61F61E77040" modified="2024-11-27" version="1">MRGRILIPLPNTGSMGRKRNNFYMRSIFLLALCIFGLLQYHNFNYLDSRDTVLGDAVTNDSDDAILAMVPAPLHKYLTPHSRNHSGAATTGAMGVLGAPLLLNSSSSGAATATTISFDVYHPPNISEIKRQIVRYNDMQLVLNEDTFGPLQNDSVIIVVQVHTRITYLRHLIVSLAQAQDITKVLLVFSHDYYDEDINDLVQQIDFCKVLQIFYPYSIQTHPHEYPGVDPNDCQRNIKKDQAMISNCNNALYPDLYGHYREAKFTQTKHHWIWKANRVFNELEVTRYHTGLVLFLEEDHYVAEDFLYLLAMMQKRTKDLCPQCNVLSLGTYLKTFNYYTYHSKVEVMPWVSSKHNMGFAFNRTTWTNIRKCARHFCTYDDYNWDWSLQHVSQQCLQRKLHAMIVKGPRVFHIGECGVHHKNKNCESNQVISKVQHVLRIARTSHQLFPRSLTLTVPSLSKKTKLRKGNGGWGDLRDHELCLNMTLATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9B3</accession>
    <name>A0AAU9F9B3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphoribosyl pyrophosphate synthase-associated protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_10371</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF92283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9F9B3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000031">
        <property type="entry name" value="Probable PRS4-ribose-phosphate pyrophosphokinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF53">
        <property type="entry name" value="GH23275P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="4">
        <location>
            <begin position="9"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="201"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="218"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF92283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="357" mass="39493" checksum="DF334D76C62CD8E8" modified="2024-11-27" version="1">MDNTSTSDIVIINGNSHPDLANMVAERMGLKNGGCSVFHKSNRETIVEISDSVRGKDIYIIQTGTKDANNNIMELLIMAYACKTSSARSIVGVIPYLPYSKQCKMRKRGCIVSKLLAKMMCTSGLTHIITMDLHQKEIQGFFDIPVDNLRASPFLLQYIQESIPEYRNSVIVARNPGVAKKANSYAERLRLGLAVIHGEQKEAESDEVDGRYSPPPTSSSRQRTTSVSVGVPEHPVKVKPPLTIVGDVNGRIAIMVDDLIDDVQSFVAAAEMLKENGACKIYVLATHGLLSSDAPRLLDESCIDEIVVTNTIPHEIQKLQCHKIKTIDISILIAEAIRRIHNKESMSYLFRNVTLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F9B9</accession>
    <name>A0AAU9F9B9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphoribosyl pyrophosphate synthase-associated protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_10371</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F9B9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000031">
        <property type="entry name" value="Probable PRS4-ribose-phosphate pyrophosphokinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF53">
        <property type="entry name" value="GH23275P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
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            <dbReference type="Pfam" id="PF13793"/>
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    <sequence length="388" mass="43019" checksum="064884485ECDDE31" modified="2024-11-27" version="1">MLLQRNKLGSVRPPKRCRSDMDNTSTSDIVIINGNSHPDLANMVAERMGLKNGGCSVFHKSNRETIVEISDSVRGKDIYIIQTGTKDANNNIMELLIMAYACKTSSARSIVGVIPYLPYSKQCKMRKRGCIVSKLLAKMMCTSGLTHIITMDLHQKEIQGFFDIPVDNLRASPFLLQYIQESIPEYRNSVIVARNPGVAKKANSYAERLRLGLAVIHGEQKEAESDEVDGRYSPPPTRNSTPQHAPISSSSRQRTTSVSVGVPEHPVKVKPPLTIVGDVNGRIAIMVDDLIDDVQSFVAAAEMLKENGACKIYVLATHGLLSSDAPRLLDESCIDEIVVTNTIPHEIQKLQCHKIKTIDISILIAEAIRRIHNKESMSYLFRNVTLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <strain evidence="4 5">RF1</strain>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
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            <begin position="1"/>
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        </location>
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        <source>
            <dbReference type="EMBL" id="BFF92728.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="822" mass="84233" checksum="D8BE6D083B2BE833" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAP5</accession>
    <name>A0AAU9FAP5_DROMD</name>
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        </submittedName>
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        <name evidence="18" type="ORF">DMAD_10753</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="18 19">RF1</strain>
        </source>
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            <location evidence="3">Neuron projection</location>
        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="1">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Perikaryon</location>
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        <subcellularLocation>
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        </subcellularLocation>
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        <text evidence="4">Belongs to the FMR1 family.</text>
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        <property type="protein sequence ID" value="BFF92771.1"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:animal organ development"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6" id="KW-0678">Repressor</keyword>
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    <feature type="domain" description="Agenet-like" evidence="17">
        <location>
            <begin position="4"/>
            <end position="49"/>
        </location>
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        <location>
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        <location>
            <begin position="382"/>
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        </location>
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        <location>
            <begin position="384"/>
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        <location>
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            <end position="412"/>
        </location>
    </feature>
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        <location>
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        </location>
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            <begin position="622"/>
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        <location>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="699" mass="78102" checksum="1D19C8A9CB4F785F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAQ2</accession>
    <name>A0AAU9FAQ2_DROMD</name>
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        <recommendedName>
            <fullName evidence="17">Mitogen-activated protein kinase kinase kinase 4</fullName>
            <ecNumber evidence="4">2.7.11.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">MAPK/ERK kinase kinase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_10771</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
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            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
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        <property type="protein sequence ID" value="BFF92793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0046872">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
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        <property type="entry name" value="MEKK4_N"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 22" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="1227"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="24"/>
            <end position="98"/>
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    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="20">
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            <begin position="1130"/>
            <end position="1173"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="24"/>
            <end position="38"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="451"/>
            <end position="463"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="1064"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1075"/>
            <end position="1090"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1164"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="1256"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00083883"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF92793.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
    <sequence length="1514" mass="170036" checksum="6EFBFFEC374BDBEB" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FAQ5</accession>
    <name>A0AAU9FAQ5_DROMD</name>
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        <submittedName>
            <fullName evidence="4">Collagen alpha chain2</fullName>
        </submittedName>
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        <name evidence="4" type="ORF">DMAD_10719</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050938">
        <property type="entry name" value="Collagen_Structural_Proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456:SF6">
        <property type="entry name" value="COLLAGEN ALPHA-1(XXIII) CHAIN-LIKE ISOFORM X2"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456">
        <property type="entry name" value="SI:CH211-266K2.1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FILAMIN_REPEAT"/>
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    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
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    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="45"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
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            <begin position="169"/>
            <end position="272"/>
        </location>
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        <location>
            <begin position="279"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
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            <end position="477"/>
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    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="478"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="574"/>
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    <feature type="repeat" description="Filamin" evidence="7">
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            <begin position="668"/>
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    <feature type="repeat" description="Filamin" evidence="7">
        <location>
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    <feature type="repeat" description="Filamin" evidence="7">
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    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="960"/>
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        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1053"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1152"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1248"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1352"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1453"/>
            <end position="1545"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1543"/>
            <end position="1633"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1636"/>
            <end position="1728"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1726"/>
            <end position="1826"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1829"/>
            <end position="1921"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1923"/>
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        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="2018"/>
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    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
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            <begin position="2146"/>
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            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="8"/>
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    </feature>
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            <begin position="22"/>
            <end position="33"/>
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    </feature>
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            <dbReference type="ARBA" id="ARBA00009238"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF92785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2240" mass="242457" checksum="7DF879A7D66E459B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FB14</accession>
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        <submittedName>
            <fullName evidence="7">Transmembrane protein 245</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="ORF">DMAD_10829</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
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    </dbReference>
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        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="TRANSMEMBRANE PROTEIN 245"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF92863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="843" mass="94656" checksum="4D0F72BDE6AC2F7B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FB17</accession>
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        <recommendedName>
            <fullName evidence="15">Papilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_10846</name>
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    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the papilin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF92890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd22639">
        <property type="entry name" value="Kunitz_papilin_lacunin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22637">
        <property type="entry name" value="Kunitz_papilin_mig6-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00199">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000020">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000107">
        <property type="entry name" value="Myosin, light chain kinase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000005">
        <property type="entry name" value="Pancreatic trypsin inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000017">
        <property type="entry name" value="papilin isoform X2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
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    <keyword evidence="3" id="KW-0964">Secreted</keyword>
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            <begin position="2454"/>
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    <sequence length="2685" mass="287859" checksum="B3759B232BA7C0EE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FB20</accession>
    <name>A0AAU9FB20_DROMD</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Pterygota</taxon>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FB33</accession>
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        <name type="scientific">Drosophila madeirensis</name>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF92921.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDZ0</accession>
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                <person name="Yamamoto D."/>
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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</entry>
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                <person name="Yamamoto D."/>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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    </dbReference>
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    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="5" id="KW-0807">Transducer</keyword>
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        <location>
            <begin position="121"/>
            <end position="126"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
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        <location>
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        <ligand>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEB0</accession>
    <name>A0AAU9FEB0_DROMD</name>
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        <submittedName>
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        <name evidence="15" type="ORF">DMAD_11779</name>
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        <name type="scientific">Drosophila madeirensis</name>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF94054.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="594" mass="66147" checksum="18632CA2E94032FE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEB5</accession>
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        <recommendedName>
            <fullName evidence="9">Angiotensin-converting enzyme</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="11" type="ORF">DMAD_11830</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:carboxypeptidase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:peptidyl-dipeptidase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001548">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10514">
        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10514:SF44">
        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01401">
        <property type="entry name" value="Peptidase_M2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PEPDIPTASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52011">
        <property type="entry name" value="PEPTIDASE_M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3 6 8" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043953154" description="Angiotensin-converting enzyme" evidence="10">
        <location>
            <begin position="17"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) (complex) asparagine" evidence="5">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="7">
        <location>
            <position position="123"/>
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        <location>
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            <end position="142"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6 8">
        <location>
            <begin position="341"/>
            <end position="359"/>
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        <location>
            <begin position="527"/>
            <end position="545"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00008139"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601548-5"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF94121.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="612" mass="70585" checksum="1E91D4628EE54ED8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJA7</accession>
    <name>A0AAU9FJA7_DROMD</name>
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            <fullName evidence="2">Glyoxylate reductase/hydroxypyruvate reductase</fullName>
        </recommendedName>
    </protein>
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        <name evidence="6" type="ORF">DMAD_13064</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
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        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="entry name" value="Glyoxylate/hydroxypyruvate reductase B"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
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        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
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        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
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        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF119">
        <property type="entry name" value="FI03731P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    </dbReference>
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        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="9"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="114"/>
            <end position="294"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00073306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF95722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="326" mass="35715" checksum="274003D6DA824831" modified="2024-11-27" version="1">MSTGKAYKVLITHPEVPQEGIDLLKENCEIIQVQSLPTNRAELLQKIRGVDGVLWGGHEALNAEVLDAAGPQLKSISTMSAGIDYVDVPEVKKRKIPLGHTPTVLNTAVADLAVGLLIAAGRRFHEGRRKIETDNWENYHLNWMLGQDIRDSKVGFYGFGGIGQAIAKRLSGFDIDQVLYTTRRRVHKEIEQELNAKKVDFDTLLAQSDFVVIASPLTADTQGVFNTTAFNKMKETAVLINIGRGKIVNQDDLYEALKSNKIFAAGLDVTDPEPLSAKDKLLSLDNVVVLPHIGSATKRTRAEMATIAAHNVLRGLVGEPMLSPAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJA8</accession>
    <name>A0AAU9FJA8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rap1 GTPase-activating protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_13014</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJA8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11210:FF:000003">
        <property type="entry name" value="RAP1 GTPase activating protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003109">
        <property type="entry name" value="GoLoco_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050989">
        <property type="entry name" value="Rap1_Ran_GAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711:SF32">
        <property type="entry name" value="RAP GTPASE ACTIVATING PROTEIN 1, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711">
        <property type="entry name" value="RAP GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02188">
        <property type="entry name" value="GoLoco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21022">
        <property type="entry name" value="Rap-GAP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00390">
        <property type="entry name" value="GoLoco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50877">
        <property type="entry name" value="GOLOCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="4">
        <location>
            <begin position="428"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="20"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="168"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="660"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="703"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="177"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="220"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="703"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="740"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="780"/>
            <end position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00057316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF95656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="958" mass="102781" checksum="07AFE71242347924" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJB1</accession>
    <name>A0AAU9FJB1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">GTPase-activating protein skywalker</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_13078</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJB1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000035">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="7">
        <location>
            <begin position="89"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="8">
        <location>
            <begin position="394"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF95745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="573" mass="65678" checksum="58E3EE86F05C9C22" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FJC7</accession>
    <name>A0AAU9FJC7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Allatostatin C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_13174</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF95863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FJC7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043650440" description="Allatostatin C" evidence="2">
        <location>
            <begin position="25"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF95863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13521" checksum="D93E92FB2A07C244" modified="2024-11-27" version="1">MMKFVQILLCYGLLLALLFSVSEARPSTAETGPDADGLGGQEGEDVRGAYGGGYDMPAQAIYPNIPMDRLQMLFAQYRPTAYLRTPSYGNMNELYRLPESKRQVRYRQCYFNPISCFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMK4</accession>
    <name>A0AAU9FMK4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Tenascin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_05398</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Synapse</location>
            <location evidence="17">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tenascin family. Teneurin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099559">
        <property type="term" value="P:maintenance of alignment of postsynaptic density and presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048790">
        <property type="term" value="P:maintenance of presynaptic active zone structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034116">
        <property type="term" value="P:positive regulation of heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040017">
        <property type="term" value="P:positive regulation of locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001941">
        <property type="term" value="P:postsynaptic membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097090">
        <property type="term" value="P:presynaptic membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034110">
        <property type="term" value="P:regulation of homotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000331">
        <property type="term" value="P:regulation of terminal button organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051124">
        <property type="term" value="P:synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016200">
        <property type="term" value="P:synaptic target attraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048499">
        <property type="term" value="P:synaptic vesicle membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.180.10.10:FF:000008">
        <property type="entry name" value="Odz, odd Oz/ten-m homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000021">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000474">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000013">
        <property type="entry name" value="Teneurin transmembrane protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000033">
        <property type="entry name" value="teneurin-a isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000560">
        <property type="entry name" value="Teneurin-m-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000189">
        <property type="entry name" value="Teneurin-m-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.180.10.10">
        <property type="entry name" value="RHS repeat-associated core"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057627">
        <property type="entry name" value="FN-plug_TEN1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056823">
        <property type="entry name" value="TEN-like_YD-shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056822">
        <property type="entry name" value="TEN_NHL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056820">
        <property type="entry name" value="TEN_TTR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051216">
        <property type="entry name" value="Teneurin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057629">
        <property type="entry name" value="Teneurin1-4_GBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028916">
        <property type="entry name" value="Tox-GHH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006530">
        <property type="entry name" value="YD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01643">
        <property type="entry name" value="YD_repeat_2x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219">
        <property type="entry name" value="TENEURIN AND N-ACETYLGLUCOSAMINE-1-PHOSPHODIESTER ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219:SF72">
        <property type="entry name" value="TENEURIN-M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25024">
        <property type="entry name" value="EGF_TEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24329">
        <property type="entry name" value="FN-plug_TEN1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23093">
        <property type="entry name" value="GBD_Tenm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25021">
        <property type="entry name" value="TEN_NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25023">
        <property type="entry name" value="TEN_YD-shell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23538">
        <property type="entry name" value="Teneurin_ABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15636">
        <property type="entry name" value="Tox-GHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25020">
        <property type="entry name" value="TTR_TEN1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 19" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <keyword evidence="5" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 21" id="KW-1133">Transmembrane helix</keyword>
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</entry>
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                <person name="Yamamoto D."/>
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            <authorList>
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                <person name="Yamamoto D."/>
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</entry>
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                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="41"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="322"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF96880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="407" mass="43480" checksum="5D2254F08BDB24B8" modified="2024-11-27" version="1">MVHIIELVGQPKVEFAQQVTMNRALQLKPAENESRSEHLDRKLFVGMLSKQQTEDDVRQIFHPFGSIEECTILRGPDGTSKGCAFVKFGTQQEAQSAIANLHGSQTMPGASSSLVVKYADTEKERQIRRMQQMAGHMNLLNPFVFNQFSPYGAYAQQQQQAALMAAAATAPGSAAAYMNPMAALATQIPHGLNGTGQPPSLPSPTMPNFSMGANTPNGQPGGAAAAAAAAAADGVFTNGIPQTAFPGHPLHLTIPPQGLPNGDAATLQHAFPGLPPFPGVAFPAVYGQFPQALPPPLAAVAPTQREDFLMFPGCSISGPEGCNLFIYHLPQEFGDAELMQMFLPFGNVISSKVFIDRATNQSKCFGFVSFDNPASAQAAIQAMNGFQIGMKRLKVQLKRPKDASRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FML6</accession>
    <name>A0AAU9FML6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein Teyrha-meyrha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_05631</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FML6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052443">
        <property type="entry name" value="E3_ubiq-ligase_RNF220-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031824">
        <property type="entry name" value="RNF220_mid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13459">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF220 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13459:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF220 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15926">
        <property type="entry name" value="RNF220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="E3 ubiquitin-protein ligase RNF220 middle" evidence="2">
        <location>
            <begin position="332"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="629"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="764"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="684"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="695"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="764"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="798"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF97157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="815" mass="87701" checksum="88441BFE55DF8869" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FML7</accession>
    <name>A0AAU9FML7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Lon protease homolog, mitochondrial</fullName>
            <ecNumber evidence="11">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_05623</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homohexamer or homoheptamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12 13">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="11"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FML7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070407">
        <property type="term" value="P:oxidation-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000043">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5270:FF:000001">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1480:FF:000002">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000015">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03120">
        <property type="entry name" value="lonm_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027503">
        <property type="entry name" value="Lonm_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718">
        <property type="entry name" value="LON PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718:SF2">
        <property type="entry name" value="LON PROTEASE HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 11 12" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="16">
        <location>
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            <authorList>
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                <person name="Yamamoto D."/>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <location>
            <begin position="311"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="578"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF97159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="664" mass="74285" checksum="8E8F2A7D2C09DF9E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMN4</accession>
    <name>A0AAU9FMN4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="9">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_05341</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="9"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMN4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106435">
        <property type="term" value="F:carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00312">
        <property type="entry name" value="Esterase_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000378">
        <property type="entry name" value="Carboxylic ester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043101558" description="Carboxylic ester hydrolase" evidence="9">
        <location>
            <begin position="23"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="10">
        <location>
            <begin position="29"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF96781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="515" mass="57676" checksum="86E5D51B8B65D471" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMN5</accession>
    <name>A0AAU9FMN5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_05589</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAMHD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMN5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="2">
        <location>
            <begin position="50"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF97103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="368" mass="42988" checksum="036E0D42D05E6F59" modified="2024-11-27" version="1">MLIEDEVHGVIELPSHIQEIVEHPLFQRLKKVHQLGLVPWVLEPHANQKRYDHCLGTYKSAQDHMRAIERNTKVKAKLPNWCRMAVEIAALLHDIGHGPFSHVWESACQQQFDHEENALHCVDRIFKDARHSELVALRDEGCGRGVQLIKALILGDTEYLKFPMMGYAYIFDIVHNNRCGLDVDKWDYLRRDNKRLKLLSPAEMDFDKVFLKARISLDGQRIEYRYEDYHRIYALFEARGRLHVEAYQHPRNAVAEQIFADLVQRLAPQLVDLRSNDAGWLNLDDEHVLELIQNDPMAAYFREPKRVVELPFFNRSDAKVETVCRIIEAPGKAMKPDNAFAFYGSRRNKRQINRCLTDTSFNKCFQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMS3</accession>
    <name>A0AAU9FMS3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Polyglutamine-repeat protein pqn-41</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_05677</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMS3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12407">
        <property type="entry name" value="RRM_FOX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000004">
        <property type="entry name" value="RNA binding fox-1 homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034237">
        <property type="entry name" value="FOX1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047131">
        <property type="entry name" value="RBFOX1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597">
        <property type="entry name" value="ATAXIN 2-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597:SF22">
        <property type="entry name" value="RNA-BINDING FOX PROTEIN 1, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="364"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="158"/>
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                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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                <person name="Yamamoto D."/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <location>
            <begin position="284"/>
            <end position="296"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF98174.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="506" mass="58536" checksum="88EFFE3DE84A80FA" modified="2024-11-27" version="1">MRQQMPLRLRLLGIVLYVSILTSWLYSRFMNIFGDYWPSKYLLIHGCCCGIAYFLAEHGTHLRRFSLIGLDVGLQIWQSISESTFTSHKIRLVAVTTFVSGLIQSCYGNLVLDDDWGQGWSAGKMFWAWLLTTLVLSKLHMIKNLYEMVMQQQLPLILKPRKENASYMERLWEPIILWLCRQQMCQVPEGMTELQLSFYMALDVECLFGFRLLAASEFNEAAYSQSDPLCPTIFSPTQLHTSWLNLRELIMGMVDEFLANMKNAMEDNQRIERTPKNGLRDATRPSTTKRAQNMPKSQPFRDQLIQGNLKFRFRTVGAPSCQPIKWCPTCGRPSEPIDVIVLQLNEICQCPNELQMLVIWCRGIWECLPVLPATVHYFCDTNPMEKINHVLRHAEHLDDVLRGLVRICIRSLKEDKCRLTEPDLGRILEALLQVEQHLYAARNLQMARGGRLCGTHQKLIEGIEHCMFSMLQKFSPHLDFLVQDNVLLKMIKCRMESLGIPHTHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRQ8</accession>
    <name>A0AAU9FRQ8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein heavy chain, cytoplasmic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Dynein heavy chain, cytosolic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_06397</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Consists of at least two heavy chains and a number of intermediate and light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048469">
        <property type="term" value="P:cell maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051642">
        <property type="term" value="P:centrosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051293">
        <property type="term" value="P:establishment of spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051235">
        <property type="term" value="P:maintenance of location"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072384">
        <property type="term" value="P:organelle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008090">
        <property type="term" value="P:retrograde axonal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein 1 heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000122">
        <property type="entry name" value="Cytoplasmic dynein 1 heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000002">
        <property type="entry name" value="cytoplasmic dynein 1 heavy chain 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000001">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.130:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000005">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000003">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000001">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000071">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000517">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000003">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000004">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000004">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000373">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001956">
        <property type="entry name" value="Dynein cytoplasmic 1 heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054354">
        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532:SF13">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 HEAVY CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532">
        <property type="entry name" value="MALE FERTILITY FACTOR KL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0243">Dynein</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="1903"/>
            <end position="2052"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2215"/>
            <end position="2366"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2585"/>
            <end position="2735"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2927"/>
            <end position="3093"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="537"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="1283"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3206"/>
            <end position="3251"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3401"/>
            <end position="3484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF98151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="4654" mass="531640" checksum="20881FFFBD6097CF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRR3</accession>
    <name>A0AAU9FRR3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">HECT-type E3 ubiquitin transferase NEDD4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_06775</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRR3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019871">
        <property type="term" value="F:sodium channel inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008586">
        <property type="term" value="P:imaginal disc-derived wing vein morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045879">
        <property type="term" value="P:negative regulation of smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048260">
        <property type="term" value="P:positive regulation of receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048814">
        <property type="term" value="P:regulation of dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04033">
        <property type="entry name" value="C2_NEDD4_NEDD4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000037">
        <property type="entry name" value="E3 ubiquitin-protein ligase nedd-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000096">
        <property type="entry name" value="E3 ubiquitin-protein ligase Nedd-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000021">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024928">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF440">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEDD-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001569">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="14"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="15">
        <location>
            <begin position="209"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="15">
        <location>
            <begin position="498"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="15">
        <location>
            <begin position="549"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="16">
        <location>
            <begin position="641"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="170"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="265"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="394"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="527"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="307"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="437"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="454"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="11 12">
        <location>
            <position position="943"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001569-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF98689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="976" mass="112076" checksum="EC83FE3160A7A4DE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRR9</accession>
    <name>A0AAU9FRR9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein phosphatase 1 regulatory subunit 12B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Myosin phosphatase-targeting subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_06462</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Regulates myosin phosphatase activity. Augments Ca(2+) sensitivity of the contractile apparatus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12B mediates binding to myosin. Isoform 3 and isoform 4 bind PPP1R12A, but not isoform 1 of PPP1R12B itself. Binds IL16.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NRARP family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRR9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019208">
        <property type="term" value="F:phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="IPD_PPP1R12"/>
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    <sequence length="1149" mass="124022" checksum="B445FA1F4102F97B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="17 18">RF1</strain>
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    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF98223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="620" mass="69365" checksum="DD368857A6B714E9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRS2</accession>
    <name>A0AAU9FRS2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cell division protein ZipA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DMAD_06434</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRS2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF98206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="133" mass="14740" checksum="F4F056930FDD40C5" modified="2024-11-27" version="1">MLLALALVGVVAGAQLPDSATQGPNPQDIATPEPEYIDIEEPLPAAAAPAPAPRPVFAAAPRPVIAAPIARPIARPIQQTFIQQQPQPQPIAQRVQYLPPQQQQLVGHSFNSRSGYQYRRPVYVRRFYRLRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRS3</accession>
    <name>A0AAU9FRS3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Neuronal membrane glycoprotein M6-a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_06787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRS3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001614">
        <property type="entry name" value="Myelin_PLP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11683:SF12">
        <property type="entry name" value="M6, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11683">
        <property type="entry name" value="MYELIN PROTEOLIPID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01275">
        <property type="entry name" value="Myelin_PLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF98704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="248" mass="28363" checksum="BD84EBBFD4266CF8" modified="2024-11-27" version="1">MGECCQSCMARIPYATMIATLMCLLGVGIFCFTMYRGASLTVIMVDQVFHLRLIWIEAVQMIFVIIGAGMAALGFMILFVGFLATGATRYKVYRAWRSRVGGRISCAVLMGITYLLNFVWSLILCFLVVVTFIYTMFWNMCSSVEHSQSCIDLTQFHFMFPPNTKLEDMKVCEKYEIKAFCKDGVENAEVMFILATLSTLLVILSLVHYLMCLSANYAHIRDHEKFQELQEMQNLNELEYSATSKDRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRS7</accession>
    <name>A0AAU9FRS7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="9">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_06775</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="9"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FRS7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019871">
        <property type="term" value="F:sodium channel inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008586">
        <property type="term" value="P:imaginal disc-derived wing vein morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045879">
        <property type="term" value="P:negative regulation of smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048260">
        <property type="term" value="P:positive regulation of receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048814">
        <property type="term" value="P:regulation of dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04033">
        <property type="entry name" value="C2_NEDD4_NEDD4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000037">
        <property type="entry name" value="E3 ubiquitin-protein ligase nedd-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000096">
        <property type="entry name" value="E3 ubiquitin-protein ligase Nedd-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000021">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024928">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF440">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEDD-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001569">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
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    </dbReference>
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</entry>
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    <sequence length="508" mass="53913" checksum="1FCF56381831AF57" modified="2024-11-27" version="1">MTSGEWDLRALKALEEEEQREFLALSQRNLFEPLPSDGGGAGAGAGGGGAKPALTYAQLHPHLLKLPTIDECQLIAAKQQSVGSSGNGHGSLSGLRFTRALSLSAHSLSPRGGGGGGGGGGGFGKKLRARLVGAKSSGSLSPSRHSHSYSVSPVYTPPPMRRCATLHHTQPVRKTFKNLQQLQDRKRQASQNAAPPMPLPGQLQRITGTGKTYWKYGANSTAASIIGQRTRRQQQHGTEPPVESNAASDLELNAEQLEADVLDSATETGSCSVPLPAADTNEVLRLMLSAASSAVTVTPTAAAAAAATGGTMSGPATVSVPAAATLRRYQAIGDVVEETVAYEAFHKPKSFERGALLRAGSLTVEESNSNSARNSPGRAMQSTLTGGRRIFKSASLDWPGEPQTQTQTPPQPQAQQQQQINGLTTNPLEIADQQTMEARYKQREWEHMHHHHQSHHQQQPDASLGIGMAMPGGHTIMDDELEQHIKHCSCSCNHMGYGNSMDYQVSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FRT4</accession>
    <name>A0AAU9FRT4_DROMD</name>
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        <submittedName>
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        <name evidence="13" type="ORF">DMAD_06449</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13 14">RF1</strain>
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    </reference>
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        <subcellularLocation>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF98238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="4147" mass="455256" checksum="1076255691B48AE5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FS27</accession>
    <name>A0AAU9FS27_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Calcium-binding mitochondrial carrier protein SCaMC-1-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_06852</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FS27"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000028">
        <property type="entry name" value="Putative calcium-binding mitochondrial carrier protein scamc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000003">
        <property type="entry name" value="Putative calcium-binding mitochondrial carrier protein scamc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000320">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="124"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="189"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="225"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="295"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="388"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="484"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="22"/>
            <end position="37"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF98792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="595" mass="66525" checksum="7DD942796D9119BB" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FS38</accession>
    <name>A0AAU9FS38_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Augmin complex subunit msd1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_06724</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FS38"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF98593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="140" mass="15923" checksum="EDEBFD704E45BB3F" modified="2024-11-27" version="1">MTDAVDDLLAGLVANRQTMQRQSAIIDEILEKSNNTLIHIESRNKALAPATAAQEQEKVYNVKPACKQALVKILQNYKQLMQQHRDEREDKHSALDGCLAYRQRVEQLASTMRKLVALCDTVHQMKTHQEDLEAEEQVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FS40</accession>
    <name>A0AAU9FS40_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_06498</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043542">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040181">
        <property type="entry name" value="PKHG5/7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13217:SF11">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13217">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="1060"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="637"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="717"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="809"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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</entry>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1344"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="400"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="483"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="563"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="608"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="999"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1030"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1345"/>
            <end position="1363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF98839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1406" mass="152607" checksum="40216E215D9ECB95" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVD4</accession>
    <name>A0AAU9FVD4_DROMD</name>
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        <submittedName>
            <fullName evidence="18">Spectrin alpha chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_07205</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the spectrin family.</text>
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        <property type="protein sequence ID" value="BFF99260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045169">
        <property type="term" value="C:fusome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016328">
        <property type="term" value="C:lateral plasma membrane"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045170">
        <property type="term" value="C:spectrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0042062">
        <property type="term" value="P:long-term strengthening of neuromuscular junction"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048790">
        <property type="term" value="P:maintenance of presynaptic active zone structure"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0007274">
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Alpha spectrin, isoform C"/>
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    </dbReference>
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        <property type="entry name" value="Spectrin repeat"/>
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    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="970"/>
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        <location>
            <begin position="2300"/>
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        <location>
            <begin position="2343"/>
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        <location>
            <begin position="1216"/>
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    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1676"/>
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        <location>
            <begin position="1927"/>
            <end position="1954"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1342"/>
            <end position="1361"/>
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    </feature>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00006826"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFF99260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2450" mass="281789" checksum="408D1271312D7631" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVD6</accession>
    <name>A0AAU9FVD6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific protein-tyrosine kinase</fullName>
            <ecNumber evidence="1">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_07498</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <property type="entry name" value="kinesin light chain isoform X1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015792">
        <property type="entry name" value="Kinesin_light_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783:SF3">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00381">
        <property type="entry name" value="KINESINLIGHT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01160">
        <property type="entry name" value="KINESIN_LIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 11" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="312"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="134"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="482"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="64"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="493"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF99453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="508" mass="58029" checksum="DAFBBEA877E15D00" modified="2024-11-27" version="1">MTQMSQDEIITNTKTVLQGLEALRVEHVSIMNGIAEVQKDNEKSDILRKNIENIELGLSEAQVMMALTSHLQNIEAEKQKLKTQVRRLHQENAWLRDELANTQQKFQASEQLVAQLEEEKKHLEFMASVKKYDENQDQDDTCEKSRTDPVVELFPDEDNEDRNNMSPTPPSQFANQTSGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKEAANLLNDALSIRGKTLGENHPAVAATLNNLAVLYGKRGKYKDAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYDEVEKYYQRALDIYESKLGPDDPNVAKTKNNLAGCYLKQGRYTEAEILYKQVLTRAHEREFGAIDSKNKPIWQVAEEREEHKFDNRENTPYGEYGGWHKAAKVDSPTVTTTLKNLGALYRRQGMFEAAETLEDCAMRSKKEAYDLAKQTKVSQLLTSNEKRRSKQIKEDMDFSEEKNGKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVD8</accession>
    <name>A0AAU9FVD8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Spectrin beta chain non-erythrocytic 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_07437</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the spectrin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21193">
        <property type="entry name" value="CH_beta_spectrin_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21194">
        <property type="entry name" value="CH_beta_spectrin_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10571">
        <property type="entry name" value="PH_beta_spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000001">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000250">
        <property type="entry name" value="Blast:Spectrin beta chain, non-erythrocytic 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000374">
        <property type="entry name" value="Blast:Spectrin beta chain, non-erythrocytic 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000200">
        <property type="entry name" value="Karst, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000303">
        <property type="entry name" value="Karst, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000210">
        <property type="entry name" value="Karst, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000288">
        <property type="entry name" value="Karst, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000007">
        <property type="entry name" value="Spectrin alpha chain non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000013">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000017">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000011">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000024">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000043">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000135">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000145">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000149">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000156">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000224">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000152">
        <property type="entry name" value="Spectrin, beta, non-erythrocytic 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000159">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000164">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000216">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000206">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000226">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041681">
        <property type="entry name" value="PH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001605">
        <property type="entry name" value="PH_dom-spectrin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15410">
        <property type="entry name" value="PH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00683">
        <property type="entry name" value="SPECTRINPH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0117">Actin capping</keyword>
    <keyword evidence="10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="17">
        <location>
            <begin position="24"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="17">
        <location>
            <begin position="145"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="831"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="16">
        <location>
            <begin position="3747"/>
            <end position="3855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
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            <taxon>Muscomorpha</taxon>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <begin position="337"/>
            <end position="355"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="SMART" id="SM01053"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG02535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="355" mass="40136" checksum="0B27C3ADCBF61037" modified="2024-11-27" version="1">MLLHSKLFTDASSRSIGALNRINFNTRFVLKTLMTICPGTVLLVFMVSLWIIASWTLRQCERFHDEEHANLLNAMWLIAITFLSVGFGDIVPNTYCGRGIAVSTGIMGAGCTALLVAVVSRKLELTRAEKHVHNFMMDTQLTKRLKNAAANVLRETWLIYKHTRLVKRVNPGRVRTHQRKFLLAIYALRKVKMDQRKLMDNANTITDMAKTQNTVYEIISDMSSRQDAIEERLTNLEDKMQNLQEHMESLPDLLSRCLTQHQERIEQRRNFLHPDTAAAPIQQPTPQSMFNAAPMLFPHSRSVPSSNNAAATYHWPTSPILPPISSRTPHLVPDTHMPSNGSAVNSYASSNKYGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3R8</accession>
    <name>A0AAU9G3R8_DROMD</name>
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        <submittedName>
            <fullName evidence="10">Probable serine/threonine-protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_02044</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10 11">RF1</strain>
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            <location evidence="1">Nucleus</location>
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        <property type="component" value="Chromosome A"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
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    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
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        <property type="entry name" value="FI18136P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
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        <location>
            <begin position="695"/>
            <end position="857"/>
        </location>
    </feature>
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        <location>
            <begin position="44"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <end position="421"/>
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            <end position="567"/>
        </location>
    </feature>
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        <location>
            <begin position="54"/>
            <end position="92"/>
        </location>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="7">
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        </location>
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        <location>
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            <end position="214"/>
        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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        </location>
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            <begin position="548"/>
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    </feature>
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006357"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG02563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="864" mass="95631" checksum="3F550398759EAE76" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3S8</accession>
    <name>A0AAU9G3S8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Proton channel OtopLc</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_02176</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the otopetrin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3S8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015252">
        <property type="term" value="F:proton channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004878">
        <property type="entry name" value="Otopetrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522:SF62">
        <property type="entry name" value="OTOPETRIN-LIKE A, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522">
        <property type="entry name" value="PROTON CHANNEL OTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03189">
        <property type="entry name" value="Otopetrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="667"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="735"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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            <end position="786"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="807"/>
            <end position="826"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="838"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="880"/>
            <end position="897"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="177"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="485"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG02749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="956" mass="104861" checksum="04D1A893A43EFE3D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3T0</accession>
    <name>A0AAU9G3T0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Amyloid-beta-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_01871</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the APP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3T0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.770:FF:000002">
        <property type="entry name" value="beta-amyloid-like protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.570.10:FF:000002">
        <property type="entry name" value="beta-amyloid-like protein isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.770">
        <property type="entry name" value="Amyloid precursor protein, E2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.140">
        <property type="entry name" value="Amyloidogenic glycoprotein, copper-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.570.10">
        <property type="entry name" value="Amyloidogenic glycoprotein, heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036669">
        <property type="entry name" value="Amyloid_Cu-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008155">
        <property type="entry name" value="Amyloid_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011178">
        <property type="entry name" value="Amyloid_glyco_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024329">
        <property type="entry name" value="Amyloid_glyco_E2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008154">
        <property type="entry name" value="Amyloid_glyco_extra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015849">
        <property type="entry name" value="Amyloid_glyco_heparin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036454">
        <property type="entry name" value="Amyloid_glyco_heparin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019745">
        <property type="entry name" value="Amyloid_glyco_intracell_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019543">
        <property type="entry name" value="APP_amyloid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019744">
        <property type="entry name" value="APP_CUBD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036176">
        <property type="entry name" value="E2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103">
        <property type="entry name" value="ALZHEIMER'S DISEASE BETA-AMYLOID RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103:SF15">
        <property type="entry name" value="AMYLOID-BETA-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10515">
        <property type="entry name" value="APP_amyloid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12924">
        <property type="entry name" value="APP_Cu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12925">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02177">
        <property type="entry name" value="APP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00006">
        <property type="entry name" value="A4_EXTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56491">
        <property type="entry name" value="A heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89811">
        <property type="entry name" value="Amyloid beta a4 protein copper binding domain (domain 2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109843">
        <property type="entry name" value="CAPPD, an extracellular domain of amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00319">
        <property type="entry name" value="APP_CUBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51869">
        <property type="entry name" value="APP_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51870">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00320">
        <property type="entry name" value="APP_INTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043930693" evidence="12">
        <location>
            <begin position="31"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="849"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="domain" description="E1" evidence="13">
        <location>
            <begin position="33"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="E2" evidence="14">
        <location>
            <begin position="432"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="GFLD subdomain" evidence="8">
        <location>
            <begin position="33"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="CuBD subdomain" evidence="8">
        <location>
            <begin position="144"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="273"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="703"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="656"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="297"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="709"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="146"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="157"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="171"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG02332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="924" mass="101770" checksum="B51A82475AB3B9B8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G3T1</accession>
    <name>A0AAU9G3T1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Cytochrome P450 4d1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_02033</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G3T1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20628">
        <property type="entry name" value="CYP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF203">
        <property type="entry name" value="CYTOCHROME P450 4D1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG02550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="514" mass="58602" checksum="DE6CE53A006A4D05" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G426</accession>
    <name>A0AAU9G426_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cytoplasmic dynein 1 intermediate chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_02105</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G426"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000552">
        <property type="entry name" value="Cytoplasmic dynein 1 intermediate chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001109">
        <property type="entry name" value="Cytoplasmic dynein 1 intermediate chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025956">
        <property type="entry name" value="DYNC1I1/DYNC1I2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF22">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 INTERMEDIATE CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11540">
        <property type="entry name" value="Dynein_IC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="505"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="17"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG02643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="645" mass="72058" checksum="E37AFA981361679E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G428</accession>
    <name>A0AAU9G428_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Furrowed</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_02249</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G428"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000101">
        <property type="entry name" value="uncharacterized protein LOC108094628 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000112">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000089">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006585">
        <property type="entry name" value="FTP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46393:SF7">
        <property type="entry name" value="COMPLEMENT C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46393">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22633">
        <property type="entry name" value="F5_F8_type_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00607">
        <property type="entry name" value="FTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="1 8" id="KW-0768">Sushi</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043594375" description="Furrowed" evidence="11">
        <location>
            <begin position="29"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="757"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="13">
        <location>
            <begin position="33"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="12">
        <location>
            <begin position="255"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="13">
        <location>
            <begin position="377"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="13">
        <location>
            <begin position="436"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="13">
        <location>
            <begin position="496"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="561"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="831"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="896"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="561"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="584"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="659"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="672"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="691"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="854"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="897"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="913"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="932"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="406"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="466"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="526"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <authorList>
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                <person name="Yamamoto D."/>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the APP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G446"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.770:FF:000002">
        <property type="entry name" value="beta-amyloid-like protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.570.10:FF:000002">
        <property type="entry name" value="beta-amyloid-like protein isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.770">
        <property type="entry name" value="Amyloid precursor protein, E2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.140">
        <property type="entry name" value="Amyloidogenic glycoprotein, copper-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.570.10">
        <property type="entry name" value="Amyloidogenic glycoprotein, heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036669">
        <property type="entry name" value="Amyloid_Cu-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008155">
        <property type="entry name" value="Amyloid_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011178">
        <property type="entry name" value="Amyloid_glyco_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024329">
        <property type="entry name" value="Amyloid_glyco_E2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008154">
        <property type="entry name" value="Amyloid_glyco_extra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015849">
        <property type="entry name" value="Amyloid_glyco_heparin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036454">
        <property type="entry name" value="Amyloid_glyco_heparin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019745">
        <property type="entry name" value="Amyloid_glyco_intracell_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019543">
        <property type="entry name" value="APP_amyloid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019744">
        <property type="entry name" value="APP_CUBD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036176">
        <property type="entry name" value="E2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103">
        <property type="entry name" value="ALZHEIMER'S DISEASE BETA-AMYLOID RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103:SF15">
        <property type="entry name" value="AMYLOID-BETA-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10515">
        <property type="entry name" value="APP_amyloid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12924">
        <property type="entry name" value="APP_Cu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12925">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02177">
        <property type="entry name" value="APP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00006">
        <property type="entry name" value="A4_EXTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56491">
        <property type="entry name" value="A heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89811">
        <property type="entry name" value="Amyloid beta a4 protein copper binding domain (domain 2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109843">
        <property type="entry name" value="CAPPD, an extracellular domain of amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00319">
        <property type="entry name" value="APP_CUBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51869">
        <property type="entry name" value="APP_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51870">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00320">
        <property type="entry name" value="APP_INTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043650459" evidence="12">
        <location>
            <begin position="31"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="853"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="domain" description="E1" evidence="13">
        <location>
            <begin position="33"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="E2" evidence="14">
        <location>
            <begin position="432"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="GFLD subdomain" evidence="8">
        <location>
            <begin position="33"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="CuBD subdomain" evidence="8">
        <location>
            <begin position="144"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="273"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="707"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="660"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="297"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="713"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="146"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="157"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="171"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG02330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="928" mass="102212" checksum="F5CC20A347870C42" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G447</accession>
    <name>A0AAU9G447_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Neurogenic protein mastermind</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_01855</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYT1 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GO" id="GO:0000978">
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008270">
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="Znf_C2H2C"/>
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    <dbReference type="InterPro" id="IPR036060">
        <property type="entry name" value="Znf_C2H2C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816:SF15">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01530">
        <property type="entry name" value="zf-C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103637">
        <property type="entry name" value="CCHHC domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51802">
        <property type="entry name" value="ZF_CCHHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="314"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="375"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="435"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="554"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="704"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="933"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1176"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1373"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1441"/>
            <end position="1540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1614"/>
            <end position="1695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="377"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="460"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="554"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="571"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="623"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="669"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="705"/>
            <end position="717"/>
        </location>
    </feature>
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    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01649">
        <property type="entry name" value="hnRNP-L_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22976">
        <property type="entry name" value="RRM_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11835">
        <property type="entry name" value="RRM_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="81"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="286"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG03597.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="520" mass="56980" checksum="3986B0EC48D45F93" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDS0</accession>
    <name>A0AAU9GDS0_DROMD</name>
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        <recommendedName>
            <fullName evidence="22">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="22">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">DMAD_04733</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26 27">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 22">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 22">Membrane</location>
            <topology evidence="2 22">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 22">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="22"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDS0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000021">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000035">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000010">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 21 22" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 20 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 22" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="880"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="922"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="963"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="991"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1031"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="24">
        <location>
            <begin position="53"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="25">
        <location>
            <begin position="813"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1140"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1152"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="1168"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="19">
        <location>
            <position position="415"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="493"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="534"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="557"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="591"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="671"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="672"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="673"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="761"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="767"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="787"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="790"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="791"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="791"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="BFG06153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1188" mass="134407" checksum="E3B5F0D48D9398B0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDS5</accession>
    <name>A0AAU9GDS5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14114">
        <property type="entry name" value="STKc_Twitchin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="5"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:000642">
        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="3"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:000505">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Protein CBR-UNC-22"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="Titin a"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Titin isoform E"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="titin isoform X1"/>
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        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000249">
        <property type="entry name" value="twitchin isoform X2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="twitchin isoform X4"/>
        <property type="match status" value="17"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:000936">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform G"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform G"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Uncharacterized protein, isoform H"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000935">
        <property type="entry name" value="Uncharacterized protein, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001001">
        <property type="entry name" value="Uncharacterized protein, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000553">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000748">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="72"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF151">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
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        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
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        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="37"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="9"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="122"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="231"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="338"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="441"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="546"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="643"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="749"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
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            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="733"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="744"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="776"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="791"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="834"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFG05856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="936" mass="101305" checksum="26CBAC01E98ED8C4" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDT2</accession>
    <name>A0AAU9GDT2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">protein kinase C</fullName>
            <ecNumber evidence="7">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">DMAD_04829</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30 31">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="7"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11622">
        <property type="entry name" value="HR1_PKN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11623">
        <property type="entry name" value="HR1_PKN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11625">
        <property type="entry name" value="HR1_PKN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05589">
        <property type="entry name" value="STKc_PKN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000001">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000002">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000058">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000008">
        <property type="entry name" value="serine/threonine-protein kinase N isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000038">
        <property type="entry name" value="serine/threonine-protein kinase N2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.160">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037313">
        <property type="entry name" value="PKN_HR1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02185">
        <property type="entry name" value="HR1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17 23 25" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 30" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="19" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="REM-1" evidence="29">
        <location>
            <begin position="44"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="29">
        <location>
            <begin position="148"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="29">
        <location>
            <begin position="244"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="1159"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="28">
        <location>
            <begin position="1419"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="574"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="831"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1032"/>
            <end position="1075"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1105"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="755"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="396"/>
            <end position="406"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="583"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="840"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="26">
        <location>
            <begin position="862"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="875"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="904"/>
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        </location>
    </feature>
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        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="1105"/>
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        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1188"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="BFG06289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1486" mass="165156" checksum="516815A5FFE22BAC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDT3</accession>
    <name>A0AAU9GDT3_DROMD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_04429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GDT3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="533"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="658"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="744"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="552"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG05767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="784" mass="86461" checksum="611BFEC0A95C6401" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GDU0</accession>
    <name>A0AAU9GDU0_DROMD</name>
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        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14114">
        <property type="entry name" value="STKc_Twitchin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000321">
        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000440">
        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000097">
        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000642">
        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000614">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001845">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001892">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000460">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000504">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000505">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000147">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002083">
        <property type="entry name" value="Protein CBR-UNC-22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000160">
        <property type="entry name" value="Titin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000003">
        <property type="entry name" value="Titin isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000012">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000127">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001003">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000249">
        <property type="entry name" value="twitchin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000056">
        <property type="entry name" value="twitchin isoform X4"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000936">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000775">
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000805">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000831">
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001034">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001033">
        <property type="entry name" value="Uncharacterized protein, isoform H"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform I"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000553">
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        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="78"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FN3_dom"/>
    </dbReference>
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        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF151">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
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        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
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        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="40"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="38"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0514">Muscle protein</keyword>
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</entry>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Yamamoto D."/>
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            <strain evidence="9 10">RF1</strain>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10 11">RF1</strain>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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            <dbReference type="EMBL" id="BFF89876.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="495" mass="56266" checksum="08E22CF8582047A9" modified="2024-11-27" version="1">MISSQLWIAVGILAWMYILWSRRRFYLLMLRVKGPAGYPLLGISINYLWKKRNMALRGNIFEKYGPTFLTWLGPVPVLTTCEPNVVQDILLSPHCMDKSQAACNAVTKTAGAGNLALRGSQWVERRKQMNPSFRHNVLMSFLPIFNGEAKSLLSLMDTFVGQGEREILPDLLNWSFRVASQTTLGSNVKENPNFINGTLIKNFNTIFELTTLSVVLPWIQNRTIATLLGIEKKITEAKLLMDELMRPIIEEKMKNTPDESQESLARRNTVINRVVQFLKSGELSYDDAKGECGIIVAAGFETTGITVFSTLTFLAMFPEYQEAVFEELKDVFPNVGDFDVSYEDLQKLVYLDRVLKESMRLIPAVPLVPRETSHDVRLSNGIVLPKGLAVSVDIFHTHRNKDVWGPEADKFNPDNFLPENISKIHQYAFIPFSKGKRICIGWKYAEINVKIALSKILRNFKLSTTFRSEDFVFIDNIGMEFGKKPLLALQRRSPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F4D2</accession>
    <name>A0AAU9F4D2_DROMD</name>
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        <submittedName>
            <fullName evidence="9">Irregular chiasm C-roughest protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="9" type="ORF">DMAD_08506</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        </lineage>
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    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Single-pass type I membrane protein</topology>
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        <property type="project" value="TreeGrafter"/>
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        </source>
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        </source>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
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            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF89840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="714" mass="79003" checksum="E6D2EFFF16D40347" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F4D5</accession>
    <name>A0AAU9F4D5_DROMD</name>
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        <submittedName>
            <fullName evidence="6">Tight junction protein ZO-1</fullName>
        </submittedName>
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        <name evidence="6" type="ORF">DMAD_08517</name>
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    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
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        </lineage>
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    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BFF89855.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        </location>
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        <location>
            <begin position="982"/>
            <end position="1083"/>
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    </feature>
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        <location>
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    </dbReference>
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        <property type="entry name" value="Mitochondrial fission 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016543">
        <property type="entry name" value="Fis1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033745">
        <property type="entry name" value="Fis1_cytosol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028061">
        <property type="entry name" value="Fis1_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028058">
        <property type="entry name" value="Fis1_TPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13247:SF0">
        <property type="entry name" value="MITOCHONDRIAL FISSION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13247">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 11 TPR REPEAT PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14853">
        <property type="entry name" value="Fis1_TPR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14852">
        <property type="entry name" value="Fis1_TPR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008835">
        <property type="entry name" value="TPR_repeat_11_Fis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9 11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR008835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF93972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="148" mass="16995" checksum="CCA60B89B41E0DD3" modified="2024-11-27" version="1">MDELLNDVVPQEDLERFEKKYHHELELDGEVTSETKFEYAFCLVRSRYTNDIRKGIMFLEDLARTHPDGRRDYIYYLAFGNARIKNYTEGLKYCKAFLEIESNDQVRSLEEYIKKQSDKEIAKGMAVAGGAALVLGGILGLGIAMSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEA2</accession>
    <name>A0AAU9FEA2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_11749</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FEA2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.20.20">
        <property type="entry name" value="Receptor L-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006211">
        <property type="entry name" value="Furin-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000494">
        <property type="entry name" value="Rcpt_L-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036941">
        <property type="entry name" value="Rcpt_L-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF3">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00757">
        <property type="entry name" value="Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01030">
        <property type="entry name" value="Recep_L_domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043773380" description="receptor protein-tyrosine kinase" evidence="16">
        <location>
            <begin position="26"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="871"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="domain" description="Receptor L-domain" evidence="18">
        <location>
            <begin position="51"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Furin-like cysteine-rich" evidence="17">
        <location>
            <begin position="185"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Receptor L-domain" evidence="18">
        <location>
            <begin position="331"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFF94015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="946" mass="108821" checksum="E7CBB22BB80B9B92" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEC1</accession>
    <name>A0AAU9FEC1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_11730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FEC1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044715">
        <property type="term" value="F:8-oxo-dGDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03676">
        <property type="entry name" value="NUDIX_Tnr3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000019">
        <property type="entry name" value="Thiamin pyrophosphokinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031804">
        <property type="entry name" value="DUF4743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15916">
        <property type="entry name" value="DUF4743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="115"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF93995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="328" mass="37187" checksum="5529D0ABE8D67064" modified="2024-11-27" version="1">MCMCFHKGDIRPFLVEGQQVGLIKSDVLKQLVKYPEVFCIRDCEQTKQGLVELNPAFRDYNERTEQVEKVLRNLRSEGLFPALQGWRDEYFEVKSEHRPLLKMERAATPLFGVRKYGVDINGYVRHPTLGLCIWLQQRSNTKETWPGKWDNMVGGGLSVGFGIKETAIKEAAEEASIPSDLVKNLVSAGCVSFFFESRQGLFPNTEYVFDLELPLDFVPHNADGEVQAFELLPAKECVERVFTPDFKTTSAPVVIDFLIRHGHITADDVVDFTQIVELLHVPLQSLYIYKTRLEQLQKQHQQLPSDDGNCNSGSSTIKPLENGHKKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEC4</accession>
    <name>A0AAU9FEC4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">LIM/homeobox protein Lhx3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_11751</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FEC4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09368">
        <property type="entry name" value="LIM1_Lhx3_Lhx4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09376">
        <property type="entry name" value="LIM2_Lhx3_Lhx4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000120">
        <property type="entry name" value="Insulin gene enhancer protein ISL-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000219">
        <property type="entry name" value="LIM/homeobox protein Lhx3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000032">
        <property type="entry name" value="LIM/homeobox protein Lhx3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049594">
        <property type="entry name" value="Lhx3/4-like_LIM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF128">
        <property type="entry name" value="LIM3, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 12" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="63"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="123"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="197"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="199"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="318"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="374"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="331"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="384"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="415"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF94018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="456" mass="51029" checksum="67DAA7BC24DCF6A0" modified="2024-11-27" version="1">MKEIKRKITSLITEKVEGGKINSMELLKLMMFKSDFLSNGKCEDRVPPINLSQLPEFLLSTIPKCGGCHELILDRFILKVLERTWHAKCLQCSECHGQLNDKCFARNGQLFCKEDFFKRYGTKCSACDMGIPPTQVVRRAQDNVYHLQCFLCAMCSRTLNTGDEFYLMEDRKLICKRDYEEAKAKGLYLDGSLDGDQPNKRPRTTITAKQLETLKTAYNNSPKPARHVREQLSQDTGLDMRVVQVWFQNRRAKEKRLKKDAGRTRWSQYFRSMKGNCSPRTDKFLDKDELKVDYDSFSHHDLSNDSYSTVNLGLDEGASPHSIRGSYMHGSSSPSQYPPSSRSPPPVGQSHTFGSYPDNIVYTNIDHAAASTLHASKSHHRLHSSNNVSDLSNDSSPEQGYPDFPPSPDSWLGDSASTNNTSSNNNNTNNNNGGSGSGAASANVTTANPGTPSVHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEC7</accession>
    <name>A0AAU9FEC7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein pigeon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_11765</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FEC7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902004">
        <property type="term" value="P:positive regulation of amyloid-beta formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="GSAP_C_dom"/>
    </dbReference>
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        <property type="entry name" value="GSAP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13630">
        <property type="entry name" value="GAMMA-SECRETASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13630:SF1">
        <property type="entry name" value="GAMMA-SECRETASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14959">
        <property type="entry name" value="GSAP-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="883"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma-secretase-activating protein C-terminal" evidence="2">
        <location>
            <begin position="695"/>
            <end position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF94036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="911" mass="102623" checksum="E1DBBA7660E6A5FC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEE1</accession>
    <name>A0AAU9FEE1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycine-rich protein DOT1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_11776</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FEE1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043493724" evidence="2">
        <location>
            <begin position="21"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF94051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="244" mass="25051" checksum="AEF79F90D8BD2F8A" modified="2024-11-27" version="1">MWKYLLICVVMSALNEESSAAGQQSRRRRGYAGGYAGGFASSGGGAGGYTGTYNSGGGGVGHSSYVGTGGGGGGGYYDYDDYDGNSPNFGIIDPYLFHQQLTNHILAQNYANQHRIQQQIAAQQAYHDNLVRQNRYRGGSGSGSGSGSSSGSYNSHRYAPSYAVASGSIGPNGHRQTAYISPANAASPNIVNRFGGSSSGGGGGGYKGVSVSSYSHSNGDGTSRRGAQTTVNDNGKVTSYSTHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEE2</accession>
    <name>A0AAU9FEE2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">DNA topoisomerase</fullName>
            <ecNumber evidence="3 13">5.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_11800</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Weakly relaxes negative supercoils and displays a distinct preference for binding single-stranded DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="3 13"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FEE2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03362">
        <property type="entry name" value="TOPRIM_TopoIA_TopoIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.290.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.140:FF:000003">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.65.10">
        <property type="entry name" value="Bacterial Topoisomerase I, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="entry name" value="Topoisomerase I, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013498">
        <property type="entry name" value="Topo_IA_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034144">
        <property type="entry name" value="TOPRIM_TopoIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390:SF21">
        <property type="entry name" value="DNA TOPOISOMERASE 3-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="Zn_ribbon_Top1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPO_IA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 13" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="15">
        <location>
            <begin position="2"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="17">
        <location>
            <begin position="164"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="GRF-type" evidence="16">
        <location>
            <begin position="969"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="domain" description="GRF-type" evidence="16">
        <location>
            <begin position="1073"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="738"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="889"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="918"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1006"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1111"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="738"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="792"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="812"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="918"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1012"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1023"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1049"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1118"/>
            <end position="1141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFF94084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1179" mass="128747" checksum="076C59EDF0EC537F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEF6</accession>
    <name>A0AAU9FEF6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nuclease HARBI1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_11786</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FEF6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF269">
        <property type="entry name" value="NUCLEASE HARBI1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="177"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF94067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="406" mass="46015" checksum="12699F4B9046A26F" modified="2024-11-27" version="1">MDSDDEVLVILAAYVAAKENEDLSNIKGQSKRRRPNCWVNPYLQLRKEKGRFQNDNMVQNPTMFYQNFNMNESEFNNLFSLVEPHLVPKRNSRPDAIPLRAKLAIALEYFATGDLQCHVGSTYRISKQHMGRTIDLVSDAISTALHGEFPKWNSENMLKWATDFMEHWHFPNCIGAIDGKHVAIRAPPNSGNAFHNSKGFHSIMLMAVCDASYRFTYIDVGAYGSETEMTAFSHCSLGKAILSGNVAFPEDSMLQGVRTPYYIVADEAFQLHKRIMKPYGARKLSKSERIFNYRLSRARRCIEKAFGILSKRWMAVQDTLRCGPDRAQNVTVACCYLHNYLMRVSPREYGARVESPLMQSPADPAAESFRGRVQDFPKHVRNTIRDYVNSAVGSVPGQNESAGVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEG0</accession>
    <name>A0AAU9FEG0_DROMD</name>
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        <submittedName>
            <fullName evidence="2">Alpha-tocopherol transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_11791</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1200">
        <property type="entry name" value="Alpha-tocopherol transfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF234">
        <property type="entry name" value="SD01558P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="111"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF94073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="309" mass="36392" checksum="9E2AC5DFA6133C61" modified="2024-11-27" version="1">MWLTMRHTSSTRTRAMPAVEHKLNVSEEEVPEHIRLIASQQGECTKTKKQTIEQFRSYILERNECQPHRNDDKYLEKFLRARYWKIENSYRLLCSYYKFREHNKGYYEKVRPLDLRHLGDSDILTVTPYRDQHGHRIIIYRFGLWRPNRVTVDDIFRATIVLQELGSLEPISQIMGGVAIFDLKDLGLEHILHLSPSVAQKMIALLVTSMPIRTSALHIVNQNWVFNAGFKIFKPFLNSAMRERLYIHGSDMSSLHKHIDPEHLPKRYGGLHDDYSYTLWLDMLKEQSTESNVQKDMEQLGFIFDSNAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEG3</accession>
    <name>A0AAU9FEG3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Brain tumor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_11762</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FEG3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19813">
        <property type="entry name" value="Bbox1_BRAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19798">
        <property type="entry name" value="Bbox2_BRAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20482">
        <property type="entry name" value="CC_brat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14959">
        <property type="entry name" value="NHL_brat_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000031">
        <property type="entry name" value="B-box type zinc finger protein ncl-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002696">
        <property type="entry name" value="Brain tumor, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050952">
        <property type="entry name" value="TRIM-NHL_E3_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104:SF41">
        <property type="entry name" value="BRAIN TUMOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NHLRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="188"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="339"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="3">
        <location>
            <begin position="785"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="3">
        <location>
            <begin position="832"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="3">
        <location>
            <begin position="878"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="3">
        <location>
            <begin position="920"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="3">
        <location>
            <begin position="963"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="29"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="76"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="164"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="558"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="271"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="78"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="164"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="558"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="584"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="597"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="613"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF94032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1055" mass="112094" checksum="3734E1B33E558B1E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEG4</accession>
    <name>A0AAU9FEG4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Leupaxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_11779</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FEG4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9FMQ0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.10.30:FF:000001">
        <property type="entry name" value="Takeout-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008:SF25">
        <property type="entry name" value="IP09473P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043818323" evidence="4">
        <location>
            <begin position="25"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF96824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="274" mass="31292" checksum="45D6895E8C669BD5" modified="2024-11-27" version="1">MSVHLERSWIVMGLLALAISLVRGDDGFLTEPHYIKECRIADKNFVNCSTQSIQQLFDKLNDGIPGLTSIKSFDPFHLNRIRITQGNSNAINLKVELANVKIVGFGHTHVLESQVFKKDYSWKTTFTLPVMKLKGDYSLFGRILLIPLNGRGQVFLDADNMTVTMHTKTRLYSKGGFSFYNVTNCRVDFKIDGLKSYFSNLFNGNKQLEDSTNKFFNDNWRMLADALYTVITQTIEDILLDVLKKIFHYIPANYFVSDIPTPEQLYGKAKPKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMQ7</accession>
    <name>A0AAU9FMQ7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">lysozyme</fullName>
            <ecNumber evidence="2">3.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_05325</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.17"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.2.1.17" evidence="2"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMQ7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16890">
        <property type="entry name" value="lyz_i"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008597">
        <property type="entry name" value="Invert_lysozyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11195">
        <property type="entry name" value="DESTABILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11195:SF22">
        <property type="entry name" value="LYSOZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05497">
        <property type="entry name" value="Destabilase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51909">
        <property type="entry name" value="LYSOZYME_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="4" id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="127"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="143"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="178"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608597-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF96756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="246" mass="26829" checksum="9865CF44AAD9162F" modified="2024-11-27" version="1">MDRTSSSSVAELGAGDVEGSKVAAHQEYAEVLSLARHRRKRNIRSWSTLDLLSVAAVVIALLLILGAIYMHLKQRPHLGRLHIARKEKGKELVDTETLPISLDVAAGVAAQSITTLRPLTVETSEDCLLCIAVVATDNQPVTCGGKPCGIYRISWLYWMDAQGTSDPDNPLTQGYEGCVPVARCAEGLVRSYVRRFGYDCDGDGLIGCRDHVMLHLNGPTGCRMGKPLPALAELRMNKCFAKKDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMR1</accession>
    <name>A0AAU9FMR1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Poly(RC)-binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_05451</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMR1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22438">
        <property type="entry name" value="KH-I_PCBP_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02396">
        <property type="entry name" value="KH-I_PCBP_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22439">
        <property type="entry name" value="KH-I_PCBP_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000003">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="22"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="105"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="312"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="546"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="573"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF96925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="592" mass="59790" checksum="209E589BB9DD95D6" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMR7</accession>
    <name>A0AAU9FMR7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein max</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Myc-associated factor X</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_05581</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAX family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMR7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11406">
        <property type="entry name" value="bHLHzip_Max"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000023">
        <property type="entry name" value="MAX isoform 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10328:SF3">
        <property type="entry name" value="PROTEIN MAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10328">
        <property type="entry name" value="PROTEIN MAX MYC-ASSOCIATED FACTOR X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="39"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="126"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="91"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF97093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="167" mass="19078" checksum="D0F9423F894EC056" modified="2024-11-27" version="1">MSMSDDDRDIDIESDDDGDSDTGLGSSRHTNTANFTQAEKRAHHNALERRRRDHIKESFTNLREAVPTLKGEKTPPQASRAQILKRTTECIQTMRKKINDNQKDIDEIKKQNIQLDQQIRAMEGAMNGDGYADLQSEDDVCSDDVEDEDLELPDSSRRNKKMKTFHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMT0</accession>
    <name>A0AAU9FMT0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Poly(RC)-binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_05451</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMT0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22438">
        <property type="entry name" value="KH-I_PCBP_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02396">
        <property type="entry name" value="KH-I_PCBP_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22439">
        <property type="entry name" value="KH-I_PCBP_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000003">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="22"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="101"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="309"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="543"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="570"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF96927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="589" mass="59414" checksum="17B84B8DF54A48EC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMT1</accession>
    <name>A0AAU9FMT1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Tenascin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_05398</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Synapse</location>
            <location evidence="17">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tenascin family. Teneurin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099559">
        <property type="term" value="P:maintenance of alignment of postsynaptic density and presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048790">
        <property type="term" value="P:maintenance of presynaptic active zone structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034116">
        <property type="term" value="P:positive regulation of heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040017">
        <property type="term" value="P:positive regulation of locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001941">
        <property type="term" value="P:postsynaptic membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097090">
        <property type="term" value="P:presynaptic membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034110">
        <property type="term" value="P:regulation of homotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000331">
        <property type="term" value="P:regulation of terminal button organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051124">
        <property type="term" value="P:synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016200">
        <property type="term" value="P:synaptic target attraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048499">
        <property type="term" value="P:synaptic vesicle membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.180.10.10:FF:000008">
        <property type="entry name" value="Odz, odd Oz/ten-m homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000021">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000474">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000013">
        <property type="entry name" value="Teneurin transmembrane protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000033">
        <property type="entry name" value="teneurin-a isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000560">
        <property type="entry name" value="Teneurin-m-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000189">
        <property type="entry name" value="Teneurin-m-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.180.10.10">
        <property type="entry name" value="RHS repeat-associated core"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057627">
        <property type="entry name" value="FN-plug_TEN1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056823">
        <property type="entry name" value="TEN-like_YD-shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056822">
        <property type="entry name" value="TEN_NHL"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">RF1</strain>
        </source>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_05344</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the adenylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="3"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMT8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000315">
        <property type="entry name" value="Adenylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006267">
        <property type="entry name" value="AK1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01360">
        <property type="entry name" value="aden_kin_iso1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF96786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="200" mass="21674" checksum="39CE902CDE754F4F" modified="2024-11-27" version="1">MSEIPIIWILGGPGCGKGTQCAKIVEKYGFTHLSSGDLLRNEVASGSDKGRQLQAVMSSGGLVSNDEVLSLLNDAISRAKGSSKGFLIDGYPREKNQGVAFEERIAPADLALYFDCSEDTMLKRIMARAAAAAVKRADDNEQTIRARLQTFKLNTSAILELYADKTLTINAERDVDDIFQEVVQAIECVLKKKAKAAAQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMU7</accession>
    <name>A0AAU9FMU7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Eukaryotic translation initiation factor 2A</fullName>
            <shortName evidence="9">eIF-2A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_05457</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the WD repeat EIF2A family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMU7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001250">
        <property type="entry name" value="Eukaryotic translation initiation factor 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011387">
        <property type="entry name" value="TIF2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017222">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9 12" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Translation initiation factor beta propellor-like" evidence="11">
        <location>
            <begin position="231"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="443"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="486"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="518"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="574"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="594"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF96945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="648" mass="71529" checksum="59A1768F8586DBF2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMU8</accession>
    <name>A0AAU9FMU8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock 70 kDa protein cognate 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_05501</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMU8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000535">
        <property type="entry name" value="Heat shock 70 kDa protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF96999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="73" mass="7786" checksum="67C406405CEC7D0D" modified="2024-11-27" version="1">MPKLPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTESERLIGDAAKNQVAMNPNNTIFVSGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMV0</accession>
    <name>A0AAU9FMV0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_05634</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMV0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10974">
        <property type="entry name" value="CE4_CDA_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.370:FF:000003">
        <property type="entry name" value="CLUMA_CG003232, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.140.10:FF:000014">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000128">
        <property type="entry name" value="Vermiform, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052740">
        <property type="entry name" value="CE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45985">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45985:SF1">
        <property type="entry name" value="VERMIFORM, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043650444" description="Chitin-binding type-2 domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="4">
        <location>
            <begin position="33"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="136"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF97163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="534" mass="60480" checksum="BAF803C750D2877A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMV2</accession>
    <name>A0AAU9FMV2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">guanylate kinase</fullName>
            <ecNumber evidence="2">2.7.4.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_05696</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the guanylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="2"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMV2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:GMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000776">
        <property type="entry name" value="Guanylate kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117:SF13">
        <property type="entry name" value="GUANYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117">
        <property type="entry name" value="GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="7">
        <location>
            <begin position="5"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF97247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="203" mass="22809" checksum="F278F68943FC38BA" modified="2024-11-27" version="1">MSQGPRPLVLCGPSGSGKSTLLKLLFAEFPNTFGFSVSHTTRSPRAGEQDGVHYYFVDRAGMEEAIASDEFIETAEFTGNLYGTSKAAVREIQKQGRVCILDIEQKGVEQIRKTDLNPILIFNNPPTINELERRLRLRGSETEETLRKRLSAAEVEISYGLTPGNFHKIINNVDIEVAYREFRDFVVQELKQQENEGISISLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMV4</accession>
    <name>A0AAU9FMV4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="22">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_05354</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:18106"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Mn(2+)(in) + ATP + H2O = Mn(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66820"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:66821"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Monomer. Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Membrane</location>
            <topology evidence="22">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Sarcoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="22"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMV4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02085">
        <property type="entry name" value="P-type_ATPase_SPCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000008">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000006">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000017">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000106">
        <property type="entry name" value="E1-E2 ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006413">
        <property type="entry name" value="P-type_ATPase_IIA_PMR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01522">
        <property type="entry name" value="ATPase-IIA2_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 22" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 22" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17 22" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 22" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="774"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="844"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="874"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="23">
        <location>
            <begin position="23"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="BFF96801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="917" mass="101269" checksum="81C710273550C8CB" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMV7</accession>
    <name>A0AAU9FMV7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">UNC93-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05477</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unc-93 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMV7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055120">
        <property type="term" value="C:striated muscle dense body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043266">
        <property type="term" value="P:regulation of potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010291">
        <property type="entry name" value="Ion_channel_UNC-93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051951">
        <property type="entry name" value="UNC-93_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19444:SF13">
        <property type="entry name" value="PROTEIN UNC-93 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19444">
        <property type="entry name" value="UNC-93 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05978">
        <property type="entry name" value="UNC-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF96970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="207" mass="23141" checksum="755842C5A605D393" modified="2024-11-27" version="1">MNNERRRSSAATEFLEVVVRPKKYYKVKHPQEQSDRGKIIVSTGERWRILKNVAIISVAMMTQYVAYQGTLNLQSSLNAKDGLGTIALSCIYLSMGISCLILPTIMIRMLTCKWTLVIGMAAFIPYIALQLYSRFYTLIPAGILLGVAAAPMWAAHATYLTQISQIYAIITSSEMDAVITLFFGIFFFAWQNADTIGNLMSSLVEFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMV8</accession>
    <name>A0AAU9FMV8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Tenascin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_05398</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Synapse</location>
            <location evidence="17">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tenascin family. Teneurin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099559">
        <property type="term" value="P:maintenance of alignment of postsynaptic density and presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048790">
        <property type="term" value="P:maintenance of presynaptic active zone structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034116">
        <property type="term" value="P:positive regulation of heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040017">
        <property type="term" value="P:positive regulation of locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001941">
        <property type="term" value="P:postsynaptic membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097090">
        <property type="term" value="P:presynaptic membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034110">
        <property type="term" value="P:regulation of homotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000331">
        <property type="term" value="P:regulation of terminal button organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051124">
        <property type="term" value="P:synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016200">
        <property type="term" value="P:synaptic target attraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048499">
        <property type="term" value="P:synaptic vesicle membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.180.10.10:FF:000008">
        <property type="entry name" value="Odz, odd Oz/ten-m homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000021">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000474">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000013">
        <property type="entry name" value="Teneurin transmembrane protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000033">
        <property type="entry name" value="teneurin-a isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000560">
        <property type="entry name" value="Teneurin-m-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000189">
        <property type="entry name" value="Teneurin-m-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.180.10.10">
        <property type="entry name" value="RHS repeat-associated core"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057627">
        <property type="entry name" value="FN-plug_TEN1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056823">
        <property type="entry name" value="TEN-like_YD-shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056822">
        <property type="entry name" value="TEN_NHL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056820">
        <property type="entry name" value="TEN_TTR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051216">
        <property type="entry name" value="Teneurin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057629">
        <property type="entry name" value="Teneurin1-4_GBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028916">
        <property type="entry name" value="Tox-GHH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006530">
        <property type="entry name" value="YD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01643">
        <property type="entry name" value="YD_repeat_2x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219">
        <property type="entry name" value="TENEURIN AND N-ACETYLGLUCOSAMINE-1-PHOSPHODIESTER ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219:SF72">
        <property type="entry name" value="TENEURIN-M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25024">
        <property type="entry name" value="EGF_TEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24329">
        <property type="entry name" value="FN-plug_TEN1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23093">
        <property type="entry name" value="GBD_Tenm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25021">
        <property type="entry name" value="TEN_NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25023">
        <property type="entry name" value="TEN_YD-shell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23538">
        <property type="entry name" value="Teneurin_ABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15636">
        <property type="entry name" value="Tox-GHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25020">
        <property type="entry name" value="TTR_TEN1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 19" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <keyword evidence="5" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="475"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="513"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="677"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="90"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="216"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="286"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="2640"/>
            <end position="2679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="216"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="2652"/>
            <end position="2672"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="501"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="535"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="681"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="703"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00084038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BFF96856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2679" mass="297548" checksum="0FB9B5C7BD8841CF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN05</accession>
    <name>A0AAU9FN05_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ionotropic receptor 75a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_05513</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FN05"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044297">
        <property type="term" value="C:cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000180">
        <property type="entry name" value="Ionotropic receptor 75a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057074">
        <property type="entry name" value="IR75A_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF32">
        <property type="entry name" value="IONOTROPIC RECEPTOR 31A, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24576">
        <property type="entry name" value="IR75A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="394"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="587"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic receptor 75a N-terminal" evidence="16">
        <location>
            <begin position="57"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="15">
        <location>
            <begin position="327"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF24576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF97015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="620" mass="72103" checksum="4EBFA90F18A359DE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN09</accession>
    <name>A0AAU9FN09_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Poly(RC)-binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_05451</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="KH-I_PCBP_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KH-I_PCBP_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22439">
        <property type="entry name" value="KH-I_PCBP_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000003">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="22"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="105"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="313"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="547"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="574"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF96929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="593" mass="59861" checksum="25FFBF3C2AEA864E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN13</accession>
    <name>A0AAU9FN13_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Hormone receptor 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_05677</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97223.1"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FN13"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12407">
        <property type="entry name" value="RRM_FOX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000375">
        <property type="entry name" value="RNA binding fox-1 homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034237">
        <property type="entry name" value="FOX1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047131">
        <property type="entry name" value="RBFOX1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597">
        <property type="entry name" value="ATAXIN 2-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597:SF22">
        <property type="entry name" value="RNA-BINDING FOX PROTEIN 1, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="476"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="157"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="196"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="269"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="322"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="607"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="673"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="197"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="277"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="341"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="611"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="673"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF97223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="929" mass="92633" checksum="D32D515C5A7C618C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN20</accession>
    <name>A0AAU9FN20_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Biogenesis of lysosome-related organelles complex 1 subunit 6</fullName>
            <shortName evidence="5">BLOC-1 subunit 6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_05750</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1) involved in pigment granule biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the BLOC1S6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FN20"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017242">
        <property type="entry name" value="BLOC-1_pallidin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028119">
        <property type="entry name" value="Snapin/Pallidin/Snn1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31328">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31328:SF2">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14712">
        <property type="entry name" value="Snapin_Pallidin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037609">
        <property type="entry name" value="BLOC-1_complex_pallidin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF97309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="170" mass="19305" checksum="48383F1AECB80EA5" modified="2024-11-27" version="1">MLKSSNFNSEQLAADQTASDQRNRLNAATKATTDNSTCWSAINQDALGGSLAAVQLSAGVLQIAEPPLDRVRSQLRELIGKQNKIYIDLSKEKYKLDCSEVARLNDMMGDVKRYKEKLTRVKREMQGIYQRTKELKKRAANVAACKQRDYQRKLERQQHEESLIGSQKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN22</accession>
    <name>A0AAU9FN22_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_05448</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017216">
        <property type="entry name" value="HPS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029438">
        <property type="entry name" value="HPS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029437">
        <property type="entry name" value="HPS3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28633:SF1">
        <property type="entry name" value="BLOC-2 COMPLEX MEMBER HPS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28633">
        <property type="entry name" value="HERMANSKY-PUDLAK SYNDROME 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14763">
        <property type="entry name" value="HPS3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14761">
        <property type="entry name" value="HPS3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BLOC-2 complex member HPS3 N-terminal" evidence="2">
        <location>
            <begin position="135"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="BLOC-2 complex member HPS3 C-terminal" evidence="3">
        <location>
            <begin position="712"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="960"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1055"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1130"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="960"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="973"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1130"/>
            <end position="1149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF96916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1513" mass="169550" checksum="AA99D6B7C37A55D2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN34</accession>
    <name>A0AAU9FN34_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="22">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_05354</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:18106"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Mn(2+)(in) + ATP + H2O = Mn(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66820"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:66821"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Monomer. Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Membrane</location>
            <topology evidence="22">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Sarcoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="22"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FN34"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02085">
        <property type="entry name" value="P-type_ATPase_SPCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000008">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000006">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000017">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000106">
        <property type="entry name" value="E1-E2 ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006413">
        <property type="entry name" value="P-type_ATPase_IIA_PMR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01522">
        <property type="entry name" value="ATPase-IIA2_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 22" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 22" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17 22" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 22" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="343"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="776"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="849"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="919"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="949"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="23">
        <location>
            <begin position="98"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF96800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="992" mass="110422" checksum="7766EB4032CCC065" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN35</accession>
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        <submittedName>
            <fullName evidence="5">Poly(RC)-binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_05451</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22439">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="22"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="105"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="267"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="501"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="528"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF96931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="547" mass="55211" checksum="887CD58E7CE13AD7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN36</accession>
    <name>A0AAU9FN36_DROMD</name>
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        <submittedName>
            <fullName evidence="9">Prominin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_05531</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prominin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FN36"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008795">
        <property type="entry name" value="Prominin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730">
        <property type="entry name" value="PROMININ PROM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730:SF1">
        <property type="entry name" value="PROMININ-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05478">
        <property type="entry name" value="Prominin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="498"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="544"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="863"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="928"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="951"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="961"/>
            <end position="979"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF97035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1022" mass="114839" checksum="5D991090BF855E51" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN53</accession>
    <name>A0AAU9FN53_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_05540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FN53"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055303">
        <property type="entry name" value="ATMIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46664">
        <property type="entry name" value="ATM INTERACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46664:SF1">
        <property type="entry name" value="ATM INTERACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="116"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF97050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="445" mass="51287" checksum="DFDD3195635CC99A" modified="2024-11-27" version="1">MEYTPNVSELLPERLYRCEDCVHQVFNNYSHYQLHLRQRHKVVLPKPNISVFYCPVERCRYHVSAIGRVEGRSFSSLRLVRQHYQKSHLPRNYECVLCGEKFLLERELEMHKCLEHQCPVCQLTYSSSSALRTHMRRKNHVSDKNVGKVAIPTLSTRKKLQEPTVPPLPPTPESSTDGAEAIAWYLPPMDVQCVGQMPEEPPLERLNIETQTEFEHLKEILVPPLRNIETQTPDDNWDQGTITDMTMAAATVVQETQTPEDNWYQSTMDEISTELRVHEQTAATTVGHIETQTPEDNWDQEVMTMVGQETQTPDDIWDQETMIEMTQVQAATSVGHIETQTTDDNWEEHTATTTVGDGFPSYPDSEPMFGAQTSANMYTQTCDELFEELGLSHIQTQTHWPDDGLYNTQHTQTCDEMLDELLDNYTSTCTQTGWLDWQKSEENDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN55</accession>
    <name>A0AAU9FN55_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">trypsin</fullName>
            <ecNumber evidence="10">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_05550</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="10"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FN55"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043773395" description="trypsin" evidence="11">
        <location>
            <begin position="32"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="63"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF97060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="314" mass="35465" checksum="5DC4F284CE69F24B" modified="2024-11-27" version="1">MEIRNLSGCVLFLLLFLLLLVSVCFTSSSNAAHHPLSELPRRRIKRISTPYFQAEKTLEMAKYVVSLRSRTPRKYFGDNHYCGGVILSRTFIMTAAHCTMDRKIQHRPRVLLVVAGTPNRLKFWRDRTVNVPVKDVYVPENFTMFNTNNIALLRLAMELPDNPYIAIASLPTHEPVYNINYTLLGWGRVYRGGPLASSILHVDVKLLDYQTCDDMLHTFKPEMMCAGNLENDQDENPCAGDTGSPLLLNGTVYGIVSYRIGCGSRSLPSVYTNVFAHLQWIDDTMKSVSIRLSSSFNILAASFVLLMNVPRINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN58</accession>
    <name>A0AAU9FN58_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Heparan sulfate 2-O-sulfotransferase pipe-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_05590</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfotransferase 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FN58"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001863">
        <property type="entry name" value="Heparan sulfate 2-o-sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007734">
        <property type="entry name" value="Heparan_SO4_2-O-STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129:SF20">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE PIPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF97104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="413" mass="47883" checksum="2D3E3676E82AEEBD" modified="2024-11-27" version="1">MSLGADRSYKMKLRDVENAFKYRRIPYPKRSVELIALLAISCTFFLFMHTNKLNSRLKEMEVKLQPSEFSALGLTGNHVSGHDAGKHDDINTLHGTYQYLKSTGQMSSLNVRDLNNTRKAQMELVFFNRVPKVGSQTFMELLRRLSERNNFQFHRDAVQKVETIRLAEDQQQEMAEVISELPEPSVFIKHVCFTNFTKFNLPKPIYLNVVRDPVERVISWFYYVRAPWYFVERKAAFPDLPLPHPAWLKKDFETCVTSGDQECTYTQGVTVEGIGDHRRQSLFFCGHDYECTPFNTVGALEKAKFAVEQQYAVVGVLEDLNTTLSVLEKYVPRFFEGVRDIYATSAEYLTKINKNNFKPPVSESVKDIVRRNFTNEIEFYQFCRQRLHKQYLAAHLPQRIITDSAHPPGFLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN62</accession>
    <name>A0AAU9FN62_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_05787</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FN62"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07843">
        <property type="entry name" value="STKc_CDC2L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000054">
        <property type="entry name" value="Cyclin-dependent kinase 11B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000523">
        <property type="entry name" value="Serine/threonine-protein kinase PITSLRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045267">
        <property type="entry name" value="CDK11/PITSLRE_STKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF107">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 11A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="590"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="121"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="306"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="360"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="902"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="306"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="376"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="386"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="471"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="530"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF97354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="991" mass="111764" checksum="74811F9BA3DE5B31" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN63</accession>
    <name>A0AAU9FN63_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Potassium voltage-gated channel protein Shal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_05614</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FN63"/>
    <dbReference type="SMR" id="A0AAU9FN63"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005250">
        <property type="term" value="F:A-type (transient outward) potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18420">
        <property type="entry name" value="BTB_POZ_Shal-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000028">
        <property type="entry name" value="potassium voltage-gated channel subfamily D member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000016">
        <property type="entry name" value="Potassium voltage-gated channel subfamily D member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000004">
        <property type="entry name" value="Potassium voltage-gated channel subfamily D member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
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    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
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            <begin position="185"/>
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        </location>
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            <dbReference type="EMBL" id="BFF97132.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="490" mass="55841" checksum="B2C184DC43608423" modified="2024-11-27" version="1">MASVAAWLPFARAAAIGWVPIASLPLPPPPVPKDRRKTDDEKLLINVSGRRFETWRNTLEKYPDTLLGSNEREFFYDEDCKEYFFDRDPDIFRHILNYYRTGKLHYPKHECLTSYDEELAFFGIMPDVIGDCCYEDYRDRKRENAERLMDDKLSENGDQNLQQLTNIRQKMWRAFENPHTSTSALVFYYVTGFFIAVSVMANVVETVPCGHRPGRAGTLPCGERYKIVFFCLDTACVMIFSAEYLLRLFAAPDRCKFVRSVMSIIDVVAILPYYIGLGITDNDDVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLVFSLAMAIIIFATVMFYAEKNVNGTNFTSIPAAFWYTIVTMTTLGYGDMVPETIAGKIVGGVCSLSGVLVIALPVPVIVSNFSRIYHQNQRSDKRKAQRKARLARIRIAKASSGAAFVNKKKAAEARWAAQESGIELDDNYRDEDIFELQHHHLLRCLEKTTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN67</accession>
    <name>A0AAU9FN67_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ral guanine nucleotide dissociation stimulator-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05494</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BFF96989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Blast:Ral guanine nucleotide dissociation stimulator-like 1"/>
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        <property type="entry name" value="Blast:Ral guanine nucleotide dissociation stimulator-like 1"/>
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    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
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        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
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        <property type="entry name" value="RASGEF_cat_dom_sf"/>
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
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        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF312">
        <property type="entry name" value="RAL GUANINE NUCLEOTIDE DISSOCIATION STIMULATOR-LIKE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ras GEF"/>
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    </dbReference>
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        <property type="entry name" value="Ubiquitin-like"/>
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    </dbReference>
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        <property type="entry name" value="RA"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="6">
        <location>
            <begin position="237"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="4">
        <location>
            <begin position="452"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="5">
        <location>
            <begin position="854"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="70"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="733"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="794"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="808"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF96989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="950" mass="106959" checksum="76290DE59ABBFAA3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN69</accession>
    <name>A0AAU9FN69_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Zinc finger protein 260-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_05507</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FN69"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000774">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001228">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF10">
        <property type="entry name" value="RIKEN CDNA 6720489N17 GENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="214"/>
            <end position="241"/>
        </location>
    </feature>
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        <location>
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        </location>
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            <begin position="270"/>
            <end position="297"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="382"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="411"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="137"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF97008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="466" mass="54314" checksum="5CE48A2466FB1501" modified="2024-11-27" version="1">MVNIYTKTRFKLHGQEQEQQLCLAYMINMCTDFNLRLGDGFPTHMCMGCIEATKAAFRWKRQFEECRRYFAQVIKAEEELCALVEEEWILTEVNEESQGPAIPEGNELTLGEPNNAMEDDRIGHCELLEPVVEAMEPIEEPKRTRQEKFCNKTNEGSTPAKKLRPRKCKGVPADEKHRQNEEESDEDETKSLLHTKFARNSNDSKLPVKKKKPFRCSQCSKAFMHKGNLEVHMRHHTGERPFPCSQCSKAFSQKCNLDHHMRLHTGERPFRCSQCSKAFVQKGNLEVHMRHHTGERPFRCSQCSKTYTQKSILDAHMRLHTGERPFRCSQCSKAFVQKGNLEVHMRHHTGERPFRCSQCSKTYTQKSILDAHMRLHTGERTYRCSQCPSAYAQMTSLNHHIMLHHPEKLPYQCSLCSKPYAKKVALEGHTMRQHTKKRSYKQRFNHKIEEGKTTREYKLRSRATGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN75</accession>
    <name>A0AAU9FN75_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DMAD_05726</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BFF97283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome J"/>
    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF97283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="247" mass="28125" checksum="B1A4A00E208E20E5" modified="2024-11-27" version="1">MNETVDEEIFIANIGALTVSTPLLTTLPHLHDSLIQHAVGLCRLLYIQDVSAWNEWSTDVAEFEDLTPHEACDKFLNEVLPIVSSYQLSTKVRKRLKLLDLHPCFRRIEQGYQYVPNDELSTCFVLKANRDFRLASALQTSSDSAQASHTVLINDAAIEQKVSVGYNFKISPETASLFSAMVSNNMELSIGQRHDLAEIHRRLRQAKQSFEDTARVLPFRRSIHDVVYNMDIFVNLRSNLCRSIEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN76</accession>
    <name>A0AAU9FN76_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Poly(RC)-binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_05451</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="KH-I_PCBP_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KH-I_PCBP_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22439">
        <property type="entry name" value="KH-I_PCBP_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="KH_dom"/>
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    </dbReference>
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        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="22"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="105"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="313"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF96921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="411" mass="43211" checksum="1A4F2EA15259A896" modified="2024-11-27" version="1">MEDNNTSSSAGGTSIKHEDPSVTLTIRLIMQGKEVGSIIGKKGEIVNRFREESGAKINISDGSCPERIVTVSGTTNAIFSAFTLITKKFEEWCSQFNDAGKIGKTQIPIRLIVPASQCGSLIGKSGSKIKEIRQTTGCSIQVASEMLPNSTERAVTLSGSAEQITQCIYQICLVMLESPPRGATIPYRPKPQVTGPVILANGQAFTIQGNYAVPTQETCPVFPLALATGGLHAGISGLADPLLKGAHLQGAVPAHHHHLQQMPDVAKNPLASLAALGLAGMNPASTGGINHTAALAALAGSQLRTANAANRAQQQQHEMTVSNDLIGCIIGKGGTKIAEIRQISGAMIRISNCEEREGGNTDRTITISGNPDSVALAQYLINMRISMETAGVPIPGYHYIAPSAIVKTPIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN77</accession>
    <name>A0AAU9FN77_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dual specificity protein kinase splA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_05695</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FN77"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016581">
        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040386">
        <property type="entry name" value="P66"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032346">
        <property type="entry name" value="P66_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13455:SF7">
        <property type="entry name" value="SIMJANG, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13455">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR P66-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16563">
        <property type="entry name" value="P66_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transcriptional repressor p66 coiled-coil MBD2-interaction" evidence="8">
        <location>
            <begin position="202"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="266"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="327"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="599"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="645"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="882"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="200"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="58"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="125"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="352"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="379"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="428"/>
            <end position="439"/>
        </location>
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        <location>
            <begin position="599"/>
            <end position="616"/>
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        <location>
            <begin position="654"/>
            <end position="674"/>
        </location>
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            <end position="694"/>
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            <end position="901"/>
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            <begin position="922"/>
            <end position="938"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF97243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="938" mass="98140" checksum="98D10B0D5B333280" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN80</accession>
    <name>A0AAU9FN80_DROMD</name>
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        <recommendedName>
            <fullName evidence="5">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="6" type="ORF">DMAD_05839</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF97421.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR056707">
        <property type="entry name" value="DUF7805"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
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    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053207">
        <property type="entry name" value="Non-NMDA_GluR_Accessory"/>
    </dbReference>
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        <property type="entry name" value="Sperma_CUB_dom_sf"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <location>
            <begin position="1236"/>
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        <location>
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        <location>
            <begin position="218"/>
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    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF97421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1297" mass="145442" checksum="E464619E6F26977D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN81</accession>
    <name>A0AAU9FN81_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein max</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Myc-associated factor X</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_05581</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAX family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11406">
        <property type="entry name" value="bHLHzip_Max"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000023">
        <property type="entry name" value="MAX isoform 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10328:SF3">
        <property type="entry name" value="PROTEIN MAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10328">
        <property type="entry name" value="PROTEIN MAX MYC-ASSOCIATED FACTOR X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="39"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="122"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="87"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF97092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="163" mass="18654" checksum="E38F55966BA3BE8F" modified="2024-11-27" version="1">MSMSDDDRDIDIESDDDGDSDTGLGSSRHTNTANFTQAEKRAHHNALERRRRDHIKESFTNLREAVPTLKGEKASRAQILKRTTECIQTMRKKINDNQKDIDEIKKQNIQLDQQIRAMEGAMNGDGYADLQSEDDVCSDDVEDEDLELPDSSRRNKKMKTFHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN89</accession>
    <name>A0AAU9FN89_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GDP-D-glucose phosphorylase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_05811</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FN89"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080048">
        <property type="term" value="F:GDP-D-glucose phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026506">
        <property type="entry name" value="GDPGP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058866">
        <property type="entry name" value="GDPGP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20884">
        <property type="entry name" value="GDP-D-GLUCOSE PHOSPHORYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20884:SF8">
        <property type="entry name" value="GDP-D-GLUCOSE PHOSPHORYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26217">
        <property type="entry name" value="GDPGP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GDPGP1-like N-terminal" evidence="1">
        <location>
            <begin position="33"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF97384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="330" mass="38097" checksum="7853B222E8B83022" modified="2024-11-27" version="1">MTKEEENPKRKRTQSKAKYVTSIQGKAPHYLNSLKVRWEQLHEIPGIFAYHLQKTRPNRVLPGECKFYAELNPDRTLKRRIPQTIENLNPTFKPKQFNFNKVDALEVMMTIDDVDKTDVQMIINRSPLTRFHTVVCPDVKNNLVQRITAHSLKFCINFMRGLDDMDIRMGYNSPGALASVNHLHFHLLHLPQDLYINQVELEELAGGYVYRLSKSMPTEAICVIFEANDDEAEVQEKDRRPGKRGNVQVFVFARSHYCVNKDLAEFNVGFCELAGYFPVGDSDKLENLTEPMVIKRIREVTGTAPQAVYTRMKQIVNGEDQSIWEQPLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVA0</accession>
    <name>A0AAU9FVA0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MADF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_07383</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FVA0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21505:SF8">
        <property type="entry name" value="DPT-YFP REPRESSOR BY OVEREXPRESSION, ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21505">
        <property type="entry name" value="MADF DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MADF" evidence="2">
        <location>
            <begin position="95"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="454"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF99490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="497" mass="56154" checksum="2C3A2BB4DA036F60" modified="2024-11-27" version="1">MREMRTMSKSISSPSAENRRQPTDSGNSMPAAVKASSSSVSQMTKSGPKKRFLSFLWRSKESKELRKQRMSLKQAKARRLKPDLNIPLEEQDIRKLIRLYCKHDNLYNPKNSYYGNKEIDEDCYIDMTRSFPGHTSEELRGYLEELKNLFEREYTIIESACRNYGEIMTPSIQYYNEFLFLVPYLCINFDKNRPRSSSDLALPKHSRSASSMLSQKKPSTTELKNSLTANEMLRSKMANLAAYGVSPCACFGKNGKRKPEKKPSAKDIKKEKKPKEQQEESKENDKPLAHEADSQVDADGHITFSPSAHKRKAPSQSQNVPESRLSIESSFYSTEKEKEVTDLGRNSHTVETAVPEEEIRQERRPSGPRICPRDAPCPAAAPVAQKISPPAAELPGGNAQQVQMLCDMIRTELTSAPDFIYFDAKWRIIEILREVHKRQLVHLKGTPICNGQPCPPAPPLKKFSPSQNSSRRGLPVPGQKVGAARACDCQFCRRPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVA1</accession>
    <name>A0AAU9FVA1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Rho GTPase-activating protein 26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_00128</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FVA1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01249">
        <property type="entry name" value="BAR-PH_GRAF_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07602">
        <property type="entry name" value="BAR_RhoGAP_OPHN1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11882">
        <property type="entry name" value="SH3_GRAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000411">
        <property type="entry name" value="Blast:Rho GTPase-activating protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000006">
        <property type="entry name" value="Rho GTPase activating protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000001">
        <property type="entry name" value="Rho GTPase-activating protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000055">
        <property type="entry name" value="rho GTPase-activating protein 26 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047234">
        <property type="entry name" value="GRAF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047225">
        <property type="entry name" value="PH_GRAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552">
        <property type="entry name" value="OLIGOPHRENIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552:SF1">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN GRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="271"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="10">
        <location>
            <begin position="393"/>
            <end position="580"/>
        </location>
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        </source>
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        </source>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1024" mass="114777" checksum="1AB49B1FB9031633" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
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        </location>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="449" mass="45433" checksum="D0E223BADACAB74E" modified="2024-11-27" version="1">MLSVQTAGCAGMERLGVPTRTSLSTPPDDRDQFRCRMRVDAHQARELLAHQQRMTTPTTTHSSSGGGSTHSGSGSGSTGLNNNSNNNNSISSNVARGGIEATTLKYGNTGSGSGSGSGNTKGESSSSMSSCGSSLQSHSNDHHQHYQYQQQQQTPRCPHHVPLPDSEYGQDRHLQIRSSYQQSEITRSYTKPPPNKTVRDVPEQISAGCGSSSYRLTTLQAAATGGIISGSSTYTASSSSASTKSKPNAITKFFSRISSPKSPPAGGSCASQPPSLASSASMSSSASSLASSSCVSTSSSASSLAAAPLPTLPISNAATLKSTACGYGTNPSAGTGPAAASSHHIFAGTPAATGTGSCTPERIPTPPLSVCVPIGSGLQLLKSSSFNLPLPFEAAAGAGAGAGAVTEGGVGVVESLLPATMSRNNSNSSMMSCHCSCNSRNCSHCGANS</sequence>
</entry>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
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        <property type="entry name" value="RRM_dom"/>
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    <dbReference type="PANTHER" id="PTHR48032">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF18">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
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    </feature>
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        </location>
    </feature>
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        </location>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="378" mass="41025" checksum="DA707F26E20EB6B0" modified="2024-11-27" version="1">MDIKIEQQQQQQQQQQQQQQVELGPCSPSEVPNDPGKMFIGGLSWQTSPESLRDYFGRYGEISEAMVMKDPTTRRSRGFGFVTFSDPNSVDKVLTQGTHELDGKKVDPKVAFPRRAHPKMVTRTKKIFVGGLSAPTTLEDVKSYFEQFGPIEDAMLMFDKQTNRHRGFGFVTFQSEDVVDKVCEIHFHEINNKMVECKKAQPKEVMLPANLAKTRAAGRSAYDNIMWGLGTLPEGFPAAAYAAYAAGRGYSGYPSFGLPYPTVDLTNGQLTPNNNTPLLTQALSVMNNYQAAAAAAHSFGPSASPHAAAAGTANRQGFPSTNSPGPTIDMYSSAGADNVGYVQATSPQPSGFPIAVSRAPLNYSPGPLIPAAFTNGYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVA9</accession>
    <name>A0AAU9FVA9_DROMD</name>
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        <submittedName>
            <fullName evidence="4">Fibrinogen C domain-containing protein 1</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ORF">DMAD_07421</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF327">
        <property type="entry name" value="FI21813P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044020910" evidence="2">
        <location>
            <begin position="18"/>
            <end position="472"/>
        </location>
    </feature>
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        <location>
            <begin position="249"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
    </feature>
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF99537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="472" mass="52749" checksum="5256DD6BB20061F6" modified="2024-11-27" version="1">MAIGIACILAAICVCEGSSLVEPPMTQAPPTLSPAHVRSRLRTNFRASPVSATTTPPRCEYKELMTNLHDRVGILATLDEDQRHRLEVIDKKLDQLVDSNAARMESLKAQQLNFQQRLDSFEHIQRLSRSTLDELKGETDLTLGPRRVRELKQKLSNRRKMKDISRSVRSAPQQYAPLPNSSAGNIDISLTDRLDALATLLTSTALSVRTVQEDVFKLTQAINRQRRLSHRRVPPNLIGQPGAAAFPAYPRLSPATSCLQYYLSVQGILKLQLTPESESFYVACDGDWTVILNRSSDEINFERGWLDYKDGFGNLAGDFFIGLNKLHALTSAAIHELRIVFEDFDGHVAYAGYSTFAIGSERELYPLSLLGRFQSDLSPSAGDSLSYHAGAKFSTIDSDNDNCLDCSCAQRYKGAGWFNNCGTSNILGQYHQQNYAKAGESGIFWDTFQGKDYSLRRVRMMIRPIGEEEARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVC4</accession>
    <name>A0AAU9FVC4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Wiskott-Aldrich syndrome protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_07575</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FVC4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043919562" evidence="2">
        <location>
            <begin position="22"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF99748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="104" mass="10527" checksum="3D3F69513187620F" modified="2024-11-27" version="1">MNDQQVLRLLLLAAAAAAASAGQIRDAAEEVEVPPAHRAAPPPQTAAGPPGGPPGGPPGGPLVGVPPPPSYPLFYQAAWLPFGRYSASIPLVPPLVVRTRATTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVC7</accession>
    <name>A0AAU9FVC7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_00150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FVC7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007259">
        <property type="term" value="P:cell surface receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001700">
        <property type="term" value="P:embryonic development via the syncytial blastoderm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031901">
        <property type="entry name" value="Unpaired"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15972">
        <property type="entry name" value="Unpaired"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG00068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="432" mass="47933" checksum="45A8201783DBB59F" modified="2024-11-27" version="1">MPAFALNATIFPQSRKGNSSQNPSWNSSSSSRRRSRSRSSLLLFIMTLAALMPLPQARHLHDQVALDYAYDEDASGSNRPAAASSPSSSSSGAEGLPAWNANPFHGLEDSFGEGSYDSDVSLSESSYEEIAQVAMRAARQELRRQRTRHARSHSLRLFSSTSQTPEWENPCGGVYQPDETLAADSDSSSQGRVIKRKHLVALRNITMSEYQFIRSSAKLEYGKYQHWQREYKFLPNMTRPTNAVKLKTWYRHMQTFVGSFSYLGRAQYKYRKEHQQNLNAVTHELHDLLVSARSMLCEIETTINASYPNSNGAKLSRVSREAMLERLRFHTPADGSQEADERDLKVSKELYIQYLDNVWKTLRRALRKQHRNSQERRQQVGAAAGVHAGAGAGAGAGAGSLRHSGSESFELGISNAAMANGSDCGGSGSVEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVC8</accession>
    <name>A0AAU9FVC8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Spectrin beta chain non-erythrocytic 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_07437</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the spectrin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21193">
        <property type="entry name" value="CH_beta_spectrin_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21194">
        <property type="entry name" value="CH_beta_spectrin_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10571">
        <property type="entry name" value="PH_beta_spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000001">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000250">
        <property type="entry name" value="Blast:Spectrin beta chain, non-erythrocytic 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000374">
        <property type="entry name" value="Blast:Spectrin beta chain, non-erythrocytic 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000200">
        <property type="entry name" value="Karst, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000303">
        <property type="entry name" value="Karst, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000210">
        <property type="entry name" value="Karst, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000288">
        <property type="entry name" value="Karst, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000007">
        <property type="entry name" value="Spectrin alpha chain non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000013">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000017">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000011">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000024">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000043">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000135">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000145">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000149">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000156">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000224">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000152">
        <property type="entry name" value="Spectrin, beta, non-erythrocytic 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000159">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000164">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000216">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000206">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000226">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041681">
        <property type="entry name" value="PH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001605">
        <property type="entry name" value="PH_dom-spectrin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15410">
        <property type="entry name" value="PH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3_2"/>
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</entry>
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                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        </source>
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        </subcellularLocation>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Lipase_N"/>
    </dbReference>
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        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LIPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043897100" evidence="5">
        <location>
            <begin position="21"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="6">
        <location>
            <begin position="52"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF99535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="324" mass="35398" checksum="1E55B1ED9A5DFBC8" modified="2024-11-27" version="1">MVKTCFCLVPLLCQLWGLHAALLDSLVSQSNIYYLQPGAAELPLQSVDQVTTVDSVKLIVHGYLASQSHGSIMPLRNAYSSQGYENVLVADWSPAATLDYPSSRFAVGKVSLVLAKELQRFLLRHSITAAAVHVVGHSLGAHIAGRIGHYFNGTLGRVTGLDPALPLFTQRSEDSLRSNAAQFVDVIHTDYPLFGDLRPRGTVDFYPNFGFAPQPGCEDVDLIAANSCSHNRAVMFYAESIGMPQNFPAIPCSLKAIKSRHVDSCLRESSRSSHNGTKSEKLDDSEVVFMGEQVSRSVTGYFYLETNGAPPYGKGRNSKYRPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVE4</accession>
    <name>A0AAU9FVE4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Serine protease 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_07324</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FVE4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000025">
        <property type="entry name" value="serine proteases 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000043">
        <property type="entry name" value="serine proteases 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8 10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="11"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF99411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="245" mass="26649" checksum="0155D56E24F5DC71" modified="2024-11-27" version="1">MPVGKSVNGRITMGYPATEGKVPYIVSLVFSNGNGGSWFCGGSIIGHTWVLTAAHCTNGASSVTIAYGSVRRQQPQFTHVVQQGDILQHEAYSTKTLNNDISLIRTPHVDFWSLVDKVELPRYEDRYNNYYGWWALLSGWGKTSDDSGVSDYLNCVDIQIADNSVCESYYGSTTITPNHVCIATPENKGSCSGDSGGPLVLHDGIRQVGIVSFGSSAGCLSNSPKGMTRVTGYLDWIRDHTGIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVE8</accession>
    <name>A0AAU9FVE8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">JNK-interacting protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein sunday driver</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07625</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK-signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Syd is required for efficient kinesin-I mediated axonal transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Forms homo- and heterooligomeric complexes. Binds the TPR motif-containing C-terminal of kinesin light chain, Klc. Pre-assembled syd scaffolding complexes are then transported as a cargo of kinesin, to the required subcellular location.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JIP scaffold family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FVE8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008432">
        <property type="term" value="F:JUN kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030159">
        <property type="term" value="F:signaling receptor complex adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1000:FF:000001">
        <property type="entry name" value="C-Jun-amino-terminal kinase-interacting protein 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1000">
        <property type="entry name" value="arf6 gtpase in complex with a specific effector, jip4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039911">
        <property type="entry name" value="JIP3/JIP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032486">
        <property type="entry name" value="JIP_LZII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034743">
        <property type="entry name" value="RH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034744">
        <property type="entry name" value="RH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13886:SF4">
        <property type="entry name" value="JNK-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13886">
        <property type="entry name" value="JNK/SAPK-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16471">
        <property type="entry name" value="JIP_LZII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09744">
        <property type="entry name" value="RH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19056">
        <property type="entry name" value="WD40_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51776">
        <property type="entry name" value="RH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51777">
        <property type="entry name" value="RH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RH1" evidence="11">
        <location>
            <begin position="18"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="RH2" evidence="12">
        <location>
            <begin position="443"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="271"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="507"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="801"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="857"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="79"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="377"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="516"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="531"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="862"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00059054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00082388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF99818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1203" mass="133865" checksum="316C8AA1E567905D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVF2</accession>
    <name>A0AAU9FVF2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_07602</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FVF2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17959">
        <property type="entry name" value="DEADc_DDX54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000865">
        <property type="entry name" value="ATP-dependent RNA helicase DDX54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012541">
        <property type="entry name" value="DBP10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033517">
        <property type="entry name" value="DDX54/DBP10_DEAD-box_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF8">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08147">
        <property type="entry name" value="DBP10CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01123">
        <property type="entry name" value="DBP10CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 16" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="15">
        <location>
            <begin position="39"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="70"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="269"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="721"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="11">
        <location>
            <begin position="39"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="759"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="820"/>
            <end position="837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF99778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="837" mass="94198" checksum="FFB273D6A2406667" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVF4</accession>
    <name>A0AAU9FVF4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07422</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FVF4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07844">
        <property type="entry name" value="STKc_PCTAIRE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000131">
        <property type="entry name" value="cyclin-dependent kinase 14 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000007">
        <property type="entry name" value="Cyclin-dependent kinase 14, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF246">
        <property type="entry name" value="ECDYSONE-INDUCED PROTEIN 63E, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="253"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="98"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="157"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF99540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="570" mass="64364" checksum="687B0A5EED709696" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVF7</accession>
    <name>A0AAU9FVF7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">JNK-interacting protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_07444</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JIP scaffold family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FVF7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008432">
        <property type="term" value="F:JUN kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007254">
        <property type="term" value="P:JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046328">
        <property type="term" value="P:regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01212">
        <property type="entry name" value="PTB_JIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11801">
        <property type="entry name" value="SH3_JIP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000032">
        <property type="entry name" value="Putative C-Jun-amino-terminal kinase-interacting protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000306">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047178">
        <property type="entry name" value="JIP1_scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47437">
        <property type="entry name" value="JNK-INTERACTING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47437:SF4">
        <property type="entry name" value="JNK-INTERACTING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="274"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="7">
        <location>
            <begin position="350"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="36"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="217"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF99582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="492" mass="53920" checksum="45A90F819A776483" modified="2024-11-27" version="1">MADSEFEEFHRPFEPHTIATFGGAGAAANKKNNNSHAFYSLIPNDDMEDSTSSKSDGDGSDQEDGIGLGMGMGMPEREPKLRQVEDDELGDGLKVTLSSDGSLDTNDSFNSHRHHPLNHQDAIGGIMGMAPNALSSAPVTIGASTDLLTPNAAATRRRRKLPEIPKNQKCGTNYGSLADEFRTGGGGMIATGPMTGARTGQQRSFLSLKCGYLMDEDSSPDSERLQSLGDVDSGHSTAHSPNDFKSMSPQITSPVSQSPFPPPFGGVPFGQLEMLEATHRGLHKFVPRHHDEIELEIGDAVYVQKEAEDLWCEGVNLRTGRQGIFPSAYAVDLDYNEFDPTVQLVKKERYLLGYLGSVETLAHKGTGVVCQAVRKIVGEYGASPTGQTCILEVSDQGLRMVDRSGPNNKKEKKPCIDYFYSLKNVSFCAFHPRDHRFIGFITKHPTVQRFACHVFKGSESTRPVAEAVGRAFQRFYQKFIETAYPIEDIYIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVF8</accession>
    <name>A0AAU9FVF8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phosphatidylinositol 4-kinase alpha</fullName>
            <ecNumber evidence="4">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_00187</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of a phosphatidylinositol 4-kinase (PI4K) complex, composed of PI4KA, EFR3 (EFR3A or EFR3B), TTC7 (TTC7A or TTC7B) and HYCC (HYCC1 or HYCC2). Interacts with TMEM150A; regulating recruitment to the plasma membrane. Interacts with TTC7A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="4"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00871">
        <property type="entry name" value="PI4Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05167">
        <property type="entry name" value="PI4Kc_III_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000005">
        <property type="entry name" value="Phosphatidylinositol 4-kinase, catalytic, alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-kinase, catalytic, alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000009">
        <property type="entry name" value="Phosphatidylinositol 4-kinase, catalytic, alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045495">
        <property type="entry name" value="PI4K_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF15">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19274">
        <property type="entry name" value="PI4K_N"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIK helical" evidence="18">
        <location>
            <begin position="1579"/>
            <end position="1754"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="17">
        <location>
            <begin position="1859"/>
            <end position="2124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFG00115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2140" mass="241030" checksum="07A9F835EE488B4B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVF9</accession>
    <name>A0AAU9FVF9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidoglycan-recognition protein LD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_07336</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FVF9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="entry name" value="Peptidoglycan recognition protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015510">
        <property type="entry name" value="PGRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006619">
        <property type="entry name" value="PGRP_domain_met/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022">
        <property type="entry name" value="PEPTIDOGLYCAN RECOGNITION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022:SF73">
        <property type="entry name" value="PEPTIDOGLYCAN-RECOGNITION PROTEIN LD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00701">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="N-acetylmuramoyl-L-alanine amidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0391">Immunity</keyword>
    <keyword evidence="2" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidoglycan recognition protein family" evidence="5">
        <location>
            <begin position="115"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF99426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="270" mass="30191" checksum="66C82F412715AFF8" modified="2024-11-27" version="1">MDPNQSAVIAALRHNPEGSANDKSCQYTTRTSARSLSQGTYGSLGTTEDIFIAVDVTESSPLLEKATPALPTLNHTKDCLNWRSVCLLVMCVCALGLAAYLLWRQTQLPGLGYRLSLIGHDIWSDRKLYGKGRLLDPLAVVSVYFTHTESEGCTEDCLELLHRLQQQRPEELPYNFLIAGDCQAFEARGWQYESSYGELPQATSLVVAFVGNFSQRPPSVCQLQTAQALLLESLKRRRIQPEYRLHMVGQAEAVQQQLQRWPRYAGGEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVG0</accession>
    <name>A0AAU9FVG0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GATA zinc finger domain-containing protein 10-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_07453</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FVG0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF99594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="227" mass="27434" checksum="5F797EE40F6A4B5D" modified="2024-11-27" version="1">MITMLNKSPLEAIESVAAARGHKIERENYLSSLRRLAKLDAESSEEPSQDTVYTSHYNKRHNWSEQAGGRQEHYQQQYHHHQQQHQPEGRYQQRYSQRGAHDRSQDDVRQSRWQSNSGGYRDHSRGRPQQEDRFGSSDNVWKQQQQQQQQQQQQRGRQQYQDRSRDRITDWRRPQQQEQRTSGTYHGDFRSRNSDWNSTSDDPEWNKPHWNGHKETRRNQCDNQKYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVG1</accession>
    <name>A0AAU9FVG1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein sprint</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_07329</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FVG1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000082">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15832:SF2">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15832">
        <property type="entry name" value="SHC (SRC HOMOLOGY DOMAIN C-TERMINAL) ADAPTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="3">
        <location>
            <begin position="300"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="70"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF99419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="433" mass="47676" checksum="C62223A2639AF333" modified="2024-11-27" version="1">MTTGNNRNGSTSSSSNSSPTADHSNSNSNSNSNSNSHSNSNSNSYFNRFDNRIAANLAAVLTGAGARFRSSSCSNRQQQQQATNSNTPRSPMHVSTISRGKHGLGLATPPSPVTLGRRRPLQLFTHANLNCNDNEKVAQTPSSDEDNSPTELNNCKRLTDKPPLVKRLTMGLLRHNEESRPLVGNMTPLRAPLDSQPVYSNGYINEDSYIDSKFGDSCRQSLTAIPMLDNVNLNDNNEFNLKKRYLRETSSANSSPKIFANRLRMNQGGASAQRSSSLANTHSNVGADEEEELDLKDACWYQAGISRDIAVEVLQSKSPGAFLVRKSSSKPGCYALTLRVPSPPGPKIANYIILRSPRGYKIKGFRKEFSSLKALITHHSVMPELLPVPLAMPRPTNISSSRRNLDDFDTYDSLQMLLKYLRAKNAETEQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVH0</accession>
    <name>A0AAU9FVH0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein strawberry notch</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_00201</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SBNO family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000282">
        <property type="entry name" value="Strawberry notch homolog 1 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057332">
        <property type="entry name" value="SBNO_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026937">
        <property type="entry name" value="SBNO_Helicase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026741">
        <property type="entry name" value="SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039187">
        <property type="entry name" value="SNO_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706:SF30">
        <property type="entry name" value="PROTEIN STRAWBERRY NOTCH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706">
        <property type="entry name" value="STRAWBERRY NOTCH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13872">
        <property type="entry name" value="AAA_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13871">
        <property type="entry name" value="Helicase_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25373">
        <property type="entry name" value="SBNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Strawberry notch AAA" evidence="7">
        <location>
            <begin position="213"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Strawberry notch helicase C" evidence="6">
        <location>
            <begin position="927"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="domain" description="SBNO alpha/beta" evidence="8">
        <location>
            <begin position="1242"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="662"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1283"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="717"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="731"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="787"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="811"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1287"/>
            <end position="1306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG00137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1461" mass="160503" checksum="8972E543052DB529" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVH2</accession>
    <name>A0AAU9FVH2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Spectrin beta chain non-erythrocytic 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_07437</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the spectrin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21193">
        <property type="entry name" value="CH_beta_spectrin_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21194">
        <property type="entry name" value="CH_beta_spectrin_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10571">
        <property type="entry name" value="PH_beta_spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000001">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000250">
        <property type="entry name" value="Blast:Spectrin beta chain, non-erythrocytic 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000374">
        <property type="entry name" value="Blast:Spectrin beta chain, non-erythrocytic 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000200">
        <property type="entry name" value="Karst, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000303">
        <property type="entry name" value="Karst, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000210">
        <property type="entry name" value="Karst, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000288">
        <property type="entry name" value="Karst, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000007">
        <property type="entry name" value="Spectrin alpha chain non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000013">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000017">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000011">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000024">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000043">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000135">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000145">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000149">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000156">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000224">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000152">
        <property type="entry name" value="Spectrin, beta, non-erythrocytic 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000159">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000164">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000216">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000206">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000226">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041681">
        <property type="entry name" value="PH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001605">
        <property type="entry name" value="PH_dom-spectrin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15410">
        <property type="entry name" value="PH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00683">
        <property type="entry name" value="SPECTRINPH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
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            <end position="41"/>
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            <begin position="447"/>
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        <source>
            <dbReference type="RuleBase" id="RU363034"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG04405.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
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            <text>K(+)(in) = K(+)(out)</text>
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    <keyword evidence="7" id="KW-0630">Potassium</keyword>
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        </location>
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    </feature>
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        <location>
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    </feature>
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    </feature>
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    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF03520"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG04004.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="995" mass="110106" checksum="7C3CE81F4600E94F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="5" type="ORF">DMAD_02968</name>
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        <name type="scientific">Drosophila madeirensis</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <location>
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        </location>
    </feature>
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        <location>
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        <location>
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            <end position="42"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="214"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03838.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="234" mass="27168" checksum="EA080B9504688CF1" modified="2024-11-27" version="1">MSNLECYGNNRSSGSRRRSRSRSQSRSKSPGGRRYHSKRKSRDRQIDGGSESANTNRCIGVFGLSTNTSQQKVRELFSKYGPIERIQMIIDAQTNRSRGFCFIYFENVADARVAKDACCGMEIDNRHIRVDYSITQRAHTPTPGVYMGRSSRNQNGRSGSPRGSRYRDYGSSSNKDSYRSGGNNRYDREYRNHRENRNDREYQRNYPKSSGSHRYVRSRSRSPVRKYRTSSRYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7A8</accession>
    <name>A0AAU9G7A8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_02659</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G7A8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07132">
        <property type="entry name" value="ALDH_F3AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000003">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="469"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG03386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="497" mass="55515" checksum="99A8FECB48B6B479" modified="2024-11-27" version="1">MANFDDTLQRARLAFASGKTRNVNFRRKQLENLLRCYEENENDIISALEADLRRPKQESLIVETEFMKNDIKHILYHLSDWVKAEKPSKSIINVMDDVQIYNDPFGVVLVIGAWNYPLQLLLVPVASAIAAGNCVVIKPSEIAGNCAKFIAEVIPKYLDNDCYPVVCGGPSETAELLKQRFDYIFYTGSTRVGKIIHAAANKHLTPTTLELGGKSPCYIDKSVELRTAVKRILWGKLINCGQTCIAPDYILCSKEMQDKFVAEAKDVLKEWYGENIQSSPDLSRVINTNNFQRLIGLMKSGRVAVGGKYDASERYIEPTILVDVKPNDPIMEEEIFGPILPIYNVESAYDAIKFINAREKPLVIYVFSNSNKLVKEFQSNTTSGGFCSNETIMHCGVDVLPFGGVGMSGMGSYHGKYGFDTFTHKKSCLGKDLSAVGEKLASARYPPYSDRKGSFLSFLLRKRRPLPNFYLTHVLAVGLGVGLTVLANYYLQKNSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7A9</accession>
    <name>A0AAU9G7A9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_03392</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G7A9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG04418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="92" mass="9597" checksum="84E8A114CABA467E" modified="2024-11-27" version="1">MDLGPDSIEDEDTLAENPNPELLESGIPLELFLSATTSALELDTVSSHAPSMSESDLLGVVGVDKLEDGDGDDQPLVLFIVSENGCDPPVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7B1</accession>
    <name>A0AAU9G7B1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KN motif and ankyrin repeat domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_02775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G7B1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030837">
        <property type="term" value="P:negative regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000243">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047184">
        <property type="entry name" value="KANK1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24168:SF21">
        <property type="entry name" value="KANK, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24168">
        <property type="entry name" value="KN MOTIF AND ANKYRIN REPEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="887"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="959"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="992"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="194"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="470"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="581"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="470"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="623"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="646"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="716"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="767"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG03547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1080" mass="118230" checksum="6863EE1F13288D6A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7B7</accession>
    <name>A0AAU9G7B7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histone acetyltransferase KAT6A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_03394</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G7B7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038875">
        <property type="entry name" value="PLA2_conodipine-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37687">
        <property type="entry name" value="AGAP006772-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37687:SF1">
        <property type="entry name" value="AGAP006772-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044020918" evidence="2">
        <location>
            <begin position="20"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="497"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="359"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG04420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="808" mass="92585" checksum="5DCF95D652BB089C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7C1</accession>
    <name>A0AAU9G7C1_DROMD</name>
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        <submittedName>
            <fullName evidence="7">Heterogeneous nuclear ribonucleoprotein L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_02818</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G7C1"/>
    <dbReference type="Proteomes" id="UP001500889">
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    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="RRM3_hnRNPL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM4_hnRNPL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000072">
        <property type="entry name" value="heterogeneous nuclear ribonucleoprotein L isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000052">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein L like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000206">
        <property type="entry name" value="Smooth, isoform L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006536">
        <property type="entry name" value="HnRNP-L/PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055204">
        <property type="entry name" value="HNRNPL_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021790">
        <property type="entry name" value="PTBP1-like_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01649">
        <property type="entry name" value="hnRNP-L_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22976">
        <property type="entry name" value="RRM_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11835">
        <property type="entry name" value="RRM_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="217"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG03601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="451" mass="48970" checksum="91B58E5E178CC52B" modified="2024-11-27" version="1">MPYNGASNGSGGAGGGGGGATIVVSEGPQNKKIRTGVQQPGENDVHMHARDVLHKICHPHGQVLRIVIFKKNGVQAMVEFDNLDAATRARENLNGADIYAGCCTLKIDYAKPEKLNVYKNEPDTSWDYTLSTGEILPIKDIGNGRSPLLQEPLYEPPLLGPGAAFPPFGAPEYHPTTPDNWKGASIHPTGLMKEPTGVVPGRNAPVAFTPQGQAQGAVMMVYGLDHDTSNTDKLFNLVCLYGNVARIKFLKTKEGTAMVQMGDSVAVERCVQHLNNIPVGTGGKIQIAFSKQNFLSEVINPFLLPDHTPSFKEYTGSKNNRFLSPAQASKNRIQPPSKILHFFNTPPGLTEDQLIGIFNIKDVPATSVRLFPLKTERSSSGLIEFPNISQAVLAIMKCNHLPIEGKGTKFPFIMKLCFSSSKSMNGAWNNATNEGMIEKENEVDSKVDIYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7C4</accession>
    <name>A0AAU9G7C4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Matrix metalloproteinase-14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_02668</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="14">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G7C4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000022">
        <property type="entry name" value="Matrix metalloproteinase 1, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000007">
        <property type="entry name" value="stromelysin-3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF291">
        <property type="entry name" value="MATRIX METALLOPROTEINASE 1, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
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</entry>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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                <person name="Yamamoto D."/>
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            <dbReference type="EMBL" id="BFG03681.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="424" mass="46574" checksum="03A60A7FEEBC3B74" modified="2024-11-27" version="1">MISTWNYKNANREAWLVLRTGSLRTRQAVFQGCLHCQFADNCGRLLGGASAPDNSGGISLEAALIDGTNMRYAAVAGMAGIRNAIGVAHDVLRYTNHSLLVGEAAARFAEGMGHEKDDLSTSSSKDVLLTWRLANCQPNFWRNVRPLANASCGKYTPLPQEKHQREMRQEYPIEQGHHDLVGFLAIDTKGYIHAASLSSGVRSRIPGRVGDAAVPGAGIYAENKVGGALATGEGDVLMRFLPALLAVEALRAGQSPDEAADAVMRRLLGRHTEFNGGLVVVSRWGIYAATCAVLDEFQFVVSGEGSVSYMPRVKILKCLDRHEIVFGGPRGIFYLAPKKIVMTGRGDFAVQRVKKVKLKEEMDFIEFSEEYIEETDIEIEMNEENPKELKEDKRSILDGSSGHLLLHQLGLAPPFPLRFPLIYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7F0</accession>
    <name>A0AAU9G7F0_DROMD</name>
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        <submittedName>
            <fullName evidence="15">Potassium channel subfamily T member 2</fullName>
        </submittedName>
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        <name evidence="15" type="ORF">DMAD_03107</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <text>K(+)(in) = K(+)(out)</text>
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            <dbReference type="ChEBI" id="CHEBI:29103"/>
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    </dbReference>
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        </source>
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        </source>
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            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1501" mass="165893" checksum="C8AA99E32F0DE020" modified="2024-11-27" version="1"> </sequence>
</entry>
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    <accession>A0AAU9G7F2</accession>
    <name>A0AAU9G7F2_DROMD</name>
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        <submittedName>
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        <name evidence="7" type="ORF">DMAD_02818</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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    </dbReference>
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            <begin position="8"/>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
    <sequence length="515" mass="56456" checksum="0754B39E1A6E465E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7F5</accession>
    <name>A0AAU9G7F5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thyrotroph embryonic factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_02689</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G7F5"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cell death specification"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF55">
        <property type="entry name" value="BZIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF07716">
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    </dbReference>
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        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Leucine zipper domain"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="6">
        <location>
            <begin position="137"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG03431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="212" mass="23560" checksum="D29CE0A14564A25C" modified="2024-11-27" version="1">MHSPAEAQAPPSTPCKSPILDVTETLSLHSEMEQEFEPKKSLYPFGTPHSHGHSQRLRLPGLSIPNLINGKNSAGAAAASTLPAFEYIAPPNHPLHALEFPLMELNHRVGVVGGMFPGFLHRRSRGEKRPIPDAQKDAKYYERRKRNNEAAKKSRDARKIREDRIAFRAALLEQENSILRAQVLALRDELQTVRQLLSATAAGGLLSMPRQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7F7</accession>
    <name>A0AAU9G7F7_DROMD</name>
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        <submittedName>
            <fullName evidence="15">Neogenin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_02711</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. DCC family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFG03459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000004">
        <property type="entry name" value="DCC isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001472">
        <property type="entry name" value="Frazzled, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001583">
        <property type="entry name" value="Frazzled, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002008">
        <property type="entry name" value="Frazzled, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Frazzled, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001797">
        <property type="entry name" value="Frazzled, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000028">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010560">
        <property type="entry name" value="Neogenin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF54">
        <property type="entry name" value="FI24025P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06583">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043728714" evidence="12">
        <location>
            <begin position="27"/>
            <end position="1569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1266"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="23"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="127"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="244"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="337"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="651"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="750"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="845"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="945"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="1048"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="1147"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="429"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="511"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="587"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1414"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1501"/>
            <end position="1526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="435"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="458"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="522"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="547"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="595"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1418"/>
            <end position="1433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG03459.1"/>
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    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1569" mass="173639" checksum="09750772B9ED7113" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7G2</accession>
    <name>A0AAU9G7G2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Potassium voltage-gated channel subfamily KQT member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_03088</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G7G2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000017">
        <property type="entry name" value="potassium voltage-gated channel subfamily KQT member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000016">
        <property type="entry name" value="Putative potassium voltage-gated channel subfamily KQT member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1910">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003937">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013821">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735:SF9">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03520">
        <property type="entry name" value="KCNQ_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01459">
        <property type="entry name" value="KCNQCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="211"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="239"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="39"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel voltage dependent KCNQ C-terminal" evidence="16">
        <location>
            <begin position="377"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="339"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="572"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="572"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG04009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="777" mass="87316" checksum="292F7EA3C44EAC1B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7G6</accession>
    <name>A0AAU9G7G6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="15">ATP-PFK</shortName>
            <shortName evidence="15">Phosphofructokinase</shortName>
            <ecNumber evidence="15">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_03389</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 15">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15 16">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="15">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 15 16">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="15"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G7G6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000001">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000003">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000043">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase, platelet type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF4">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 15 16" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 15 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="17">
        <location>
            <begin position="20"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="17">
        <location>
            <begin position="411"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="15">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="15">
        <location>
            <begin position="411"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="168"/>
        </location>
    </feature>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046872">
        <property type="entry name" value="DRHyd-ASK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046873">
        <property type="entry name" value="HisK-N-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043969">
        <property type="entry name" value="MAP3K_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025136">
        <property type="entry name" value="MAP3K_TRAF-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF394">
        <property type="entry name" value="APOPTOTIC SIGNAL-REGULATING KINASE 1, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19039">
        <property type="entry name" value="ASK_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20309">
        <property type="entry name" value="DRHyd-ASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20302">
        <property type="entry name" value="HisK-N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13281">
        <property type="entry name" value="MAP3K_TRAF_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="581"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1080"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1310"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1090"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1333"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="610"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF89125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1363" mass="154681" checksum="BB65F42E717286E1" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ERI9</accession>
    <name>A0AAU9ERI9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Calcium uptake protein 3 mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_07956</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16175">
        <property type="entry name" value="EFh_MICU3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039800">
        <property type="entry name" value="MICU1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294">
        <property type="entry name" value="EF HAND DOMAIN FAMILY A1,A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294:SF13">
        <property type="entry name" value="MITOCHONDRIAL CALCIUM UPTAKE 3, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="186"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="392"/>
            <end position="427"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF89140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="475" mass="55169" checksum="0842DD5FF433FFA8" modified="2024-11-27" version="1">MANLVARLTVKTGALIIQRTSVGVAVCRYAKYSSGPANPGGFSKKTRRLFSLLGGSAFSVAAVIAIIKLRALDNPVNAVSLKKRMRDDSELENVKLTARERRFIKFASVEFDDQLYMTPQDFLESVVEQEPRPRLKRRQLSSDEVDKYKENTPALKKGSTRLFRNLRDKGIVSYTEYLFLLSILTKPKSGFRIAFNMFDTDGNQRVDKDEFLVMERIFSGAWKEKHGEQESQELEHEVPPIEKDYVNDGEGLQRRHVVPTTLQLHFFGKRGTGVINYDNFYRFMDNLQTEVLELEFHEFSKGNSIISELDFAKILLRYTYLATDEYDVFLERLLERVKDEKGITFHDFRDFCHFLNNLDDFTIAMRMYTLADRAISKEEFSRAVKICTGYSLSQHLIDTVFAIFDADGDGLLSYKEFIAIMKDRLHRGFKVSGRFFDYNEALDAYDEPDYPIFVAWRHRVCRHLDYFIDSDALWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ERJ1</accession>
    <name>A0AAU9ERJ1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear protein MDM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_08244</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Microtubule-binding protein that negatively regulates centriole duplication. Binds to and stabilizes microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MDM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9ERJ1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046600">
        <property type="term" value="P:negative regulation of centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029136">
        <property type="entry name" value="MDM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32078">
        <property type="entry name" value="NUCLEAR PROTEIN MDM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32078:SF1">
        <property type="entry name" value="NUCLEAR PROTEIN MDM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15501">
        <property type="entry name" value="MDM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="90"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="193"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="391"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="445"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="195"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="393"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="463"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="508"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="602"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="666"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF89493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="706" mass="78117" checksum="91876781A6566D09" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ERJ6</accession>
    <name>A0AAU9ERJ6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Phosphatidylinositol-binding clathrin assembly protein LAP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_08306</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Binds clathrin and phosphatidylinositol 4,5-bisphosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PICALM/SNAP91 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9ERJ6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098894">
        <property type="term" value="C:extrinsic component of presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16985">
        <property type="entry name" value="ANTH_N_AP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000017">
        <property type="entry name" value="Phosphatidylinositol-binding clathrin assembly protein LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000001">
        <property type="entry name" value="phosphatidylinositol-binding clathrin assembly protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0168">Coated pit</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="12">
        <location>
            <begin position="16"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="580"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="660"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="682"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF89578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="702" mass="74710" checksum="3A2FB66D9F5841CE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ERK7</accession>
    <name>A0AAU9ERK7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Phosphatidylinositol-binding clathrin assembly protein LAP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_08306</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Binds clathrin and phosphatidylinositol 4,5-bisphosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PICALM/SNAP91 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9ERK7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098894">
        <property type="term" value="C:extrinsic component of presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16985">
        <property type="entry name" value="ANTH_N_AP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000017">
        <property type="entry name" value="Phosphatidylinositol-binding clathrin assembly protein LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000001">
        <property type="entry name" value="phosphatidylinositol-binding clathrin assembly protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0168">Coated pit</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="12">
        <location>
            <begin position="16"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="593"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="643"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="665"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF89591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="685" mass="72763" checksum="3CD22EEDC79F3211" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ESF5</accession>
    <name>A0AAU9ESF5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Odorant receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_08505</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Interacts with Orco. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9ESF5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF44">
        <property type="entry name" value="ODORANT RECEPTOR 13A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 11 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU351113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF89838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="390" mass="44986" checksum="B381D63E52710D23" modified="2024-11-27" version="1">MDKFMKYANFFFTSLGFEAYSTETRPPTDSLRNKLVFWGSLANLGIAGLGEFIYFYLAIAEGNLLEAVTVMSYIGFITVGLTKIFFIKWKKPALSALVQELEDLFPRGKVQEAKFKLEEYLGSCSRISFNYGLLYFILIWTFNLYPIMELIVFEKWLKVREVGKMLPYLLYIPWKWDDNWTYYMLLVALNIAGYTSASAQISTDLLLCSVATQVVMHFDYLARSMENHNLSGDWPEDSRFICEVVRYHERILRLSDVVNNILGIPLLLNFMVSSFVICFVGFQMTVGVSPDMITKLFLYLVSSMSQVYMICHYGQMVADASTGLSVAVYNQNWSHADARYQRALILIIARAQKTTYLKATIFLDITRSTMTDLLQISYKFFALLRTMYVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ESG7</accession>
    <name>A0AAU9ESG7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_08211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9ESG7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043897068" evidence="1">
        <location>
            <begin position="25"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF89455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="214" mass="24214" checksum="5887165139D3BC16" modified="2024-11-27" version="1">MSLSVAVVAASIWLIVCTFSLVQANVRFHFTLDFHVKQPANGGDETINKTILVENNQISVIDNEPTTVSPIDQWTDQEQYNDATLQKVLEARRSVQQLNAELAPLVGRSNDLAIRLKQSLRYVNEVDATLENLSNFGQLVELLRKFVSLVDALREPTNYGGVRSLEYVLLKLALEKYELNGKRQEINEYLQRAETAWTSYKNTQVVLLENSNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ESH3</accession>
    <name>A0AAU9ESH3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein detached</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_07983</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="14">Component of the dystrophin associated protein complex (DAPC). Interacts with Dg, via the Dg WW domain binding sites.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <location evidence="2">Sarcolemma</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016010">
        <property type="term" value="C:dystrophin-associated glycoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050699">
        <property type="term" value="F:WW domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008586">
        <property type="term" value="P:imaginal disc-derived wing vein morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007474">
        <property type="term" value="P:imaginal disc-derived wing vein specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048790">
        <property type="term" value="P:maintenance of presynaptic active zone structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046716">
        <property type="term" value="P:muscle cell cellular homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046928">
        <property type="term" value="P:regulation of neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048172">
        <property type="term" value="P:regulation of short-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21186">
        <property type="entry name" value="CH_DMD-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16242">
        <property type="entry name" value="EFh_DMD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02334">
        <property type="entry name" value="ZZ_dystrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000001">
        <property type="entry name" value="Dystrophin isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000151">
        <property type="entry name" value="dystrophin, isoforms A/C/F/G/H isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
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    </dbReference>
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    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="10"/>
            <end position="88"/>
        </location>
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            <begin position="295"/>
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        <location>
            <position position="12"/>
        </location>
        <ligand>
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        <location>
            <position position="15"/>
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    <sequence length="455" mass="51745" checksum="32CA04A68EBC15E1" modified="2024-11-27" version="1">MKMESNEGWIVCRVCLNNHSDSEELLQDIFTKNANPRLDQMLHLCAGIPVSADDNFPDKMCSQCVRCLRLAFKFRQTCQRSHQQILDMLTRGGNNTREESASSGEDFIAENIVKIWEEHTGQLEMSQVKLEDEEEEPREETVSFIVTPHEHDTGAEGEGEVLDAIVYDATGEDETVTTVVYEDAQTVIEDAETYTEYEEYELLTNESTPQHCTDEPAAGTQSHSEYAVEPPDDDMAVDVLSSDETSDILDEKPKTVIRTRPPRGKRTGKLGISALDGSNFEKKLGRKPRDKLSSYICDVCGNIYPTQARLTEHMKFHSGVKPHECEICGRGFVQNQQLVRHMNTHTGNRPYKCNYCPAAFADRSTKTKHHRIHTKERPYECDVCSRTFTYSDNLKFHKMIHTGEKPHVCDLCGKGFVKAYKMRLHRVTHEKHGPWKPLEATEAPGIKDEIPEFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">RF1</strain>
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        <location>
            <begin position="35"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
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        </source>
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            <dbReference type="Proteomes" id="UP001500889"/>
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    <sequence length="171" mass="19802" checksum="73E7490991896DF9" modified="2024-11-27" version="1">MAMAFDDPHWSVDLLERDHWDGLTAWRYHDRSECFSRQVEEYLHANASATRQLIADSAALNVAFRAYLTWLGFKEPNNDFNLLSKETLPGLNYSNTALFFVAYAQQHCSREESHRWSSSWAKELPSYSAQQNHTWPRLQVNGPLRNLEDFAKEFHCPIGSAMNPATKCNIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAU9F3H1_DROMD</name>
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        <recommendedName>
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        <name evidence="14" type="ORF">DMAD_09794</name>
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    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
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        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000008">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041983">
        <property type="entry name" value="ADA2-like_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016827">
        <property type="entry name" value="Ada2/TADA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055141">
        <property type="entry name" value="TADA2A_B-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374:SF63">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 2-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTOR 2 ADA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TADA2A-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025024">
        <property type="entry name" value="Transcriptional_adaptor_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="11">
        <location>
            <begin position="8"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="13">
        <location>
            <begin position="66"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="12">
        <location>
            <begin position="69"/>
            <end position="121"/>
        </location>
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</entry>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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    <accession>A0AAU9F3H6</accession>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF90934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="3015" mass="337558" checksum="C078B2D52085D12A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F3I0</accession>
    <name>A0AAU9F3I0_DROMD</name>
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        <submittedName>
            <fullName evidence="21">Glutamate-gated chloride channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_07964</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="21 22">RF1</strain>
        </source>
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        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
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        <text evidence="17">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glutamate-gated chloride channel (TC 1.A.9.4) subfamily.</text>
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    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
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    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
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            <begin position="422"/>
            <end position="441"/>
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            <begin position="34"/>
            <end position="242"/>
        </location>
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            <end position="351"/>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
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        </source>
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            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="BFF89150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="449" mass="51685" checksum="7931D7779CA0CBBC" modified="2024-11-27" version="1">MGSGHYFWAIFYFASLCSASLANNAKINFREKEKKVLDQILGAGKYDARIRPSGINGTDGPAIVRINLFVRSIMTISDIKMEYSVQLTFREQWTDERLKFDDIQGRLKYLTLTEANRVWMPDLFFSNEKEGHFHNIIMPNVYIRIFPNGSVLYSIRISLTLACPMNLKLYPLDRQICSLRMASYGWTTNDLVFLWKEGDPVQVVKNLHLPRFTLEKFLTDYCNSKTNTGEYSCLKVDLLFKREFSYYLIQIYIPCCMLVIVSWVSFWLDQGAVPARVSLGVTTLLTMATQTSGINASLPPVSYTKAIDVWTGVCLTFVFGALLEFALVNYASRSDMHKENMKKKRRDLEQASLDAASDLLDTDSNATFAMKPLVRHPGDPLALEKRLQCEVHMQAPKRPNCCKTWLSKFPTRSKRIDVISRITFPLVFALFNLVYWSTYLFREEEDETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F3I7</accession>
    <name>A0AAU9F3I7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_07974</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032394">
        <property type="entry name" value="Anoct_dimer"/>
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    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF83">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16178">
        <property type="entry name" value="Anoct_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="597"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="640"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="686"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="815"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="956"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin dimerisation" evidence="11">
        <location>
            <begin position="184"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="10">
        <location>
            <begin position="429"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="61"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF89165.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="980" mass="112720" checksum="3A8CACC0109103EC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F4F2</accession>
    <name>A0AAU9F4F2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="26">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">DMAD_08549</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30 31">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20 26">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphocholine(in) + ADP + phosphate + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:38584"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 25">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="22">Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit ATP8B1 and an accessory beta subunit TMEM30A. The flippase ATP8B1:TMEM30A complex can form an intermediate phosphoenzyme in vitro. Also interacts with beta subunit TMEM30B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="26">Membrane</location>
            <topology evidence="26">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 26">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="26"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
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    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140327">
        <property type="term" value="F:flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005548">
        <property type="term" value="F:phospholipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0007030">
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
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    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
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    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
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    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF190">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 24 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 25 26" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 24 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15 26" id="KW-1278">Translocase</keyword>
    <keyword evidence="9 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 26" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="439"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="488"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1355"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1438"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1469"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1499"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1542"/>
            <end position="1565"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="28">
        <location>
            <begin position="182"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="29">
        <location>
            <begin position="1324"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="22"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="127"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="605"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="649"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="825"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1585"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1656"/>
            <end position="1682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1697"/>
            <end position="1723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="27">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="620"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="723"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="27">
        <location>
            <begin position="1596"/>
            <end position="1605"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="1660"/>
            <end position="1682"/>
        </location>
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    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="23">
        <location>
            <position position="565"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="565"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="565"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="566"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="567"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="567"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="929"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="970"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="992"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1026"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1106"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    </feature>
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        <location>
            <position position="1107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="24">
        <location>
            <position position="1108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <location>
            <position position="1272"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1278"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="1298"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1301"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <location>
            <position position="1302"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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            <position position="1302"/>
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        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
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            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
    <sequence length="1723" mass="193653" checksum="A350BAFDA4E094F3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F4F6</accession>
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        <name type="scientific">Drosophila madeirensis</name>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
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            <begin position="167"/>
            <end position="245"/>
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            <end position="346"/>
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            <end position="105"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
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            <dbReference type="Proteomes" id="UP001500889"/>
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    <sequence length="505" mass="54460" checksum="F7A98512088002DC" modified="2024-11-27" version="1">MLAMPTLMMPATAQMTLSSQPLSVGAKPYETKLLAIHQTHMQQQQQQQATSQQQQQNGKQQAQQMTIVTTQQQQQQQQQQQHPQSQSPHLQQQQHSQQQQQHSQQQAVAAAAAAAAAHHQQQQAVAAAVCAAQQQAVVVQQQQYQIHSQQQSPQQQVLSISPKQEQFHIFVGDLSSEIETQQLREAFTPFGEISDCRVVRDPQTLKSKGYGFVSFIKKSEAESAITAMNGQWLGSRSIRTNWATRKPPASKENIKPLTFDEVYNQSSPSNCTVYVGGVNSALTALSEEVLQKTFAPYGAIQEIRVFKDKGYAFVRFSTKEAATHAIVGVHNTELNAQPVKCSWGKESGDPNNAQTIATQALNSAAAAAAGFPYGLGATAAAAYGQQLAATGCWYSPTPTYQASSATATAVTPAAASAAAVQNQFLQGIQGYHFGQYGGYQQGYMGMGVQIPATWQGVTQAQISPAQQMATGVAGTAIPQAAGVVAYPIQQFQVSPQVYPLLLQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F4G7</accession>
    <name>A0AAU9F4G7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_08577</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F4G7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14126">
        <property type="entry name" value="STKc_CK1_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000703">
        <property type="entry name" value="Casein kinase I gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022247">
        <property type="entry name" value="Casein_kinase-1_gamma_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12605">
        <property type="entry name" value="CK1gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="62"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="389"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="433"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF89945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="468" mass="52684" checksum="B367D58BFA33067A" modified="2024-11-27" version="1">MQRRERQASGGVPQTTTAGPSAGNVANATTALAGGKSSSNNMYSTRQSVSTTTGVLMVGPNFRVGKKIGCGNFGELRLGKNLYNNEHVAIKMEPMKSKAPQLHLEYRFYKLLGSHAETAPDGIPRIYHLGTCGGRYNAMVLELLGLSLEDLFNICARKFSLKTVLMIAKQLLHRIEYVHSRHLIYRDVKPENFLIGRTSTKREKIIHIIDFGLAKEYIDLDTNRHIPYREHKSLTGTARYMSINTHMGREQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRATPIEVLCDGHPEEFATYLRYVRRLDFFETPDYDFLRRLFQDLFERKGYTDEGEFDWTGKTMSTPVGSLQTGHEVIISPNKDRHNVTAKTNAKGGVAAWPDVPKPGATLGNLTPADRHGSVQVVSSTNGELNPDDPTAGHSNTPITQQPEVEVVDETKCCCFFKRKKKKSTRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F4G9</accession>
    <name>A0AAU9F4G9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein-lysine N-trimethyltransferase SMYD5</fullName>
            <ecNumber evidence="2">2.1.1.359</ecNumber>
            <ecNumber evidence="10">2.1.1.372</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">SET and MYND domain-containing protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">[histone H3]-lysine20 N-trimethyltransferase SMYD5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">[histone H4]-lysine36 N-trimethyltransferase SMYD5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_07895</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-lysyl(20)-[histone H4] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(20)-[histone H4] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:64456"/>
            <dbReference type="Rhea" id="RHEA-COMP:15554"/>
            <dbReference type="Rhea" id="RHEA-COMP:15998"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.372"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60324"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:15536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13826"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:54193"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.359" evidence="2"/>
    <dbReference type="EC" id="2.1.1.372" evidence="10"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F4G9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140955">
        <property type="term" value="F:histone H3K36 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140943">
        <property type="term" value="F:histone H4K20 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045814">
        <property type="term" value="P:negative regulation of gene expression, epigenetic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10521">
        <property type="entry name" value="SET_SMYD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000078">
        <property type="entry name" value="SMYD family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044422">
        <property type="entry name" value="SMYD5_SET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46402:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-TRIMETHYLTRANSFERASE SMYD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46402">
        <property type="entry name" value="SET AND MYND DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="19">
        <location>
            <begin position="2"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="368"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF89056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="393" mass="44716" checksum="F1318735D0D608B5" modified="2024-11-27" version="1">MNHFEIREIPGKGRAMVATKSYATDEIIFEEEPFVSSQFSWNAAYGYAACDHCMRPLETVLENVRRLASDAKLEVPLLQHDPTASWAAQFTQCQRCKVRYCSEDCLMEAQKRYHRVACMGAFRADDTHPINVLNETWKKMHYPPETGTIMLIVRLMAMYQQSSKKSEFLEQLQSFQALIVNREQKIYHKMLGENFEQQMDQLYGAFCKAFAAEEFAMFTTPDAFKTLMGIMGTNSQGIATSVLAQWVTKVSDLSLPDADKTALDTVIDDLYSKVGEFAGEFLNNEGSGLYLLQSKINHSCVPNACSTFPYSNDIVVLKALAPIQEGDEICISYLDECMLERSRHSRHKVLRENYIFVCQCLKCLAQASDPDVTSDDDEDDDEMDDYEDDDDMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F4H5</accession>
    <name>A0AAU9F4H5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Probable G-protein coupled receptor Mth-like 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_07909</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F4H5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15039">
        <property type="entry name" value="7tmB3_Methuselah-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000341">
        <property type="entry name" value="Methuselah-like 5, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052808">
        <property type="entry name" value="GPCR_Mth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46953">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR MTH-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR MTH-LIKE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043358764" evidence="6">
        <location>
            <begin position="36"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="401"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="7">
        <location>
            <begin position="206"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF89071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="487" mass="55239" checksum="3950DFA4327D4547" modified="2024-11-27" version="1">MLVKTLGAQKCSCCCCLVPILMLLLLVAVTSPAMAHISSGSSGPAPVDPGLVLVNKCCEKFEIHVDSECQQVNQTDYFQPMFTSYGGDQNRPVRFKFIIGIPNCGSMQMWPIYHYAGSSDKLVLLDDGRLRHYTNAEDEAAEQRGVQTDYEDDIAGSREPLYHDYDQGLYCIDRATSSTGEEHVLFAKICLAKKEIKWSDSNFLLRKILNPIFHGISLIILLVIAIVYFILPTLRDLVGNIVTTIAVCLMVSQAADLVRIFTELTNHVSFIVADIILCFSLLAAFFWLNSFGYYIWKTFRSRNVFLRVTDGRKYCYYSAYAWGCTGTMAALAVFAHFFLDADSYKQEQMVGEQETIGWLGICIFFAPIACTIVVNTFFYVTTRKLINRRTVYGRIAHKLKANFIMFSLMLLVMSVAWLFLIMSWLQLDGLLYAHIVVNALQSPLLLYICVLRQRHVTFLLKKSCCYNEPPSTSDWGDEMHYMNGNDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F4I0</accession>
    <name>A0AAU9F4I0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_10058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F4I0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF91864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="58644" checksum="CBEA6095D96B94F5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDI8</accession>
    <name>A0AAU9FDI8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP9 signalosome complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_11508</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Putative cop9 signalosome complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
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        <property type="entry name" value="COPS2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25983">
        <property type="entry name" value="COPS2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="9">
        <location>
            <begin position="255"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF93711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="444" mass="51555" checksum="B460FAAD077A6059" modified="2024-11-27" version="1">MSDNDDDFMCDDDEDYGLEYSEDSNSEPDVDLENQYYNSKALKEEEPKAALASFQKVLDLENGEKGEWGFKALKQMIKINFRLCNYDEMMVRYKQLLTYIKSAVTRNHSEKSINSILDYISTSKNMALLQNFYETTLDALRDAKNDRLWFKTNTKLGKLYFDRSDFTKLQKILKQLHQSCQTDDGEDDLKKGTQLLEIYALEIQMYTVQKNNKKLKALYEQSLHIKSAIPHPLIMGVIRECGGKMHLREGEFEKAHTDFFEAFKNYDESGSPRRTTCLKYLVLANMLMKSGINPFDSQEAKPYKNDPEILAMTNLVNSYQNNDINEFETILRQHRSNIMADQFIREHIEDLLRNIRTQVLIKLIRPYKNIFIPFIASALNIEPAEVESLLVSCILDDTIKGRIDQVNQVLQLDKVNSSAARYNALEKWSNQIHSLQFAVVQKMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDJ1</accession>
    <name>A0AAU9FDJ1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Liprin-alpha-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_11606</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the liprin family. Liprin-alpha subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDJ1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048786">
        <property type="term" value="C:presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09562">
        <property type="entry name" value="SAM_liprin-alpha1_2_3_4_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09565">
        <property type="entry name" value="SAM_liprin-alpha1_2_3_4_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09568">
        <property type="entry name" value="SAM_liprin-alpha1_2_3_4_repeat3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000002">
        <property type="entry name" value="PTPRF interacting protein alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000004">
        <property type="entry name" value="PTPRF interacting protein alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057892">
        <property type="entry name" value="LIP-1_CC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037620">
        <property type="entry name" value="LIP-1_SAM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037621">
        <property type="entry name" value="LIP-1_SAM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037622">
        <property type="entry name" value="LIP-1_SAM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029515">
        <property type="entry name" value="Liprin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587">
        <property type="entry name" value="LAR INTERACTING PROTEIN LIP -RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587:SF20">
        <property type="entry name" value="LIPRIN-ALPHA, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25526">
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</entry>
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</entry>
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                <person name="Yamamoto D."/>
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    </feature>
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    </feature>
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        <location>
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        </location>
    </feature>
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    </feature>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93742.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDK8</accession>
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    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_11537</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDK8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000423">
        <property type="entry name" value="Putative inorganic phosphate cotransporter-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000003">
        <property type="entry name" value="Solute carrier family 17 member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF415">
        <property type="entry name" value="AT30085P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="26"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="447"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="449"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF93748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="486" mass="53266" checksum="FCE6971C5DB2EB0B" modified="2024-11-27" version="1">MDHRDDSRPEWGKSVSKVCLLPQRVVLVLMGFFSLICAFTCRVSLSHAITILAISNKISNKTIANLTDEAPDGVCQQEVESKDAVEHIGIYTWSETEKGFILGSFYFGYLISHLPGGVLADKYGAKWVVAFCVGVTAICELLSPTSIRLGPYYLITLRVLMGIAQGPIFPGLNGLLSSWAPKKERATLGTICFGGSTMGIILSNACSGLLLHYYTWPVMFYVFGSLSVIWFFVFAIICTSSPFSHPFITPKEREYLADEVGNVNKDKPAIPWKPLLLSMPMLSLIVSQMGHDWGYYVMVTNLPLYMADVIRFSIRSNGFVTALPYVAMWIASLISGVVADWLIRSGKLRLVTVRKLFTLLSAVLPGTFMVAASYAGCDKALVVALFTISMWTMGMYYAGQKLSALDISPSYSSTITAVINGLGSFTGLFAPPIIGLLTPDAEDRLFRGSHTNGESESKSIFTQPSGHSPAFQGVSEEVSHFLRHTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDL3</accession>
    <name>A0AAU9FDL3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Venom dipeptidyl peptidase 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_11526</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Endomembrane system</location>
            <topology evidence="12">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9B family. DPPIV subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDL3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF200">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 10, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="17">
        <location>
            <begin position="123"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="16">
        <location>
            <begin position="711"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="527"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="600"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="542"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFF93732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="934" mass="104989" checksum="5C70403709179EEA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDL7</accession>
    <name>A0AAU9FDL7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein piccolo</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_11576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039892">
        <property type="entry name" value="Spa2/Sph1"/>
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    <dbReference type="InterPro" id="IPR002466">
        <property type="entry name" value="A_deamin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46516">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46516:SF1">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02137">
        <property type="entry name" value="A_deamin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00552">
        <property type="entry name" value="ADEAMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50141">
        <property type="entry name" value="A_DEAMIN_EDITASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="A to I editase" evidence="12">
        <location>
            <begin position="55"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF94095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="380" mass="43722" checksum="417149704D78C573" modified="2024-11-27" version="1">MCNCDTNLSFEDISKLCYDKFDLLPKTGKPSANQWTVLSSVVEFNRQTRDSRVVALGCGTKCIGYTRHCPKGFILNDSHAEVLARRAFLRYLYHELEYDRIFHWDAKRRSFELSEHVEYHFFSTQTPCGDACIVDESVAMGIEKSAKRPRLDVNSEMVYTGGKLIAGQLPGAITDDMEQTPGALRTKPGRGVRTLSMSCSDKLARCSLNFSCPEAHQVSVERAIYKRWQGRSSKHTRFQPQQPHIRIDVSLPFKYSQRDDWQPSPNGLVWSQLPDDLRPYEISVNGKRQGVTKKKLESAQAALAVSKFKLFQTFLNLLQSRAELRAKFQRDDSDVELMTYACCKNLCEDYQQAWRQLKEEYFQQWTFKPKGLLDFTSKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEG7</accession>
    <name>A0AAU9FEG7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha-tocopherol transfer protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_11784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FEG7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1200">
        <property type="entry name" value="Alpha-tocopherol transfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF220">
        <property type="entry name" value="LD41874P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="116"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF94063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="306" mass="35659" checksum="6CFE87622DB1BC04" modified="2024-11-27" version="1">MRVPTMLSDLDQLPSIQLGEFTLQFELGEPTAMGKEVAIKELRETPERQKEAAKDLARLLEAEKDLHYPKDNEEWLIRFLRPSKYYPESARDLIKRYYSFKVKHSDVYNNLKPSGEANIFQSNILTVFPNRDQCGRRVLVLELGKRWKHKQVSLDEVFKGAVIFLEAAMLEPETQIHGAVVIFDMDGLSLQQTWQFTPPFAKRIVDWLQDSVPLRIKAIHIVNQPKIFNVVFALFKPFLREKLRGRIIFHGTDRESLHKYMSPKCLPPAYGGILELKRVDGDEWYKLLLKCDTEFDAINSYGYKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEH0</accession>
    <name>A0AAU9FEH0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Carnitine O-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_11725</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FEH0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004092">
        <property type="term" value="F:carnitine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019254">
        <property type="term" value="P:carnitine metabolic process, CoA-linked"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000010">
        <property type="entry name" value="Carnitine O-Acetyl-Transferase, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000001">
        <property type="entry name" value="Carnitine O-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF103">
        <property type="entry name" value="CARNITINE O-ACETYL-TRANSFERASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="6">
        <location>
            <begin position="59"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF93989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="651" mass="73021" checksum="AB6AA01B2106BD1C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEH7</accession>
    <name>A0AAU9FEH7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_11769</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FEH7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007435">
        <property type="term" value="P:salivary gland morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2170:FF:000005">
        <property type="entry name" value="tyrosine-protein kinase Drl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000502">
        <property type="entry name" value="Tyrosine-protein kinase Drl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000165">
        <property type="entry name" value="Tyrosine-protein kinase RYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2170">
        <property type="entry name" value="Wnt, WIF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003306">
        <property type="entry name" value="WIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038677">
        <property type="entry name" value="WIF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF349">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02019">
        <property type="entry name" value="WIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00469">
        <property type="entry name" value="WIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50814">
        <property type="entry name" value="WIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043605676" description="receptor protein-tyrosine kinase" evidence="18">
        <location>
            <begin position="33"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="208"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="WIF" evidence="20">
        <location>
            <begin position="36"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="324"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="BFF94041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="591" mass="65435" checksum="22369B3495A2A480" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEI3</accession>
    <name>A0AAU9FEI3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha-tocopherol transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_11791</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FEI3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1200">
        <property type="entry name" value="Alpha-tocopherol transfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF234">
        <property type="entry name" value="SD01558P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="96"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF94074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="294" mass="34575" checksum="338CE698F0CA83A8" modified="2024-11-27" version="1">MPAVEHKLNVSEEEVPEHIRLIASQQGECTKTKKQTIEQFRSYILERNECQPHRNDDKYLEKFLRARYWKIENSYRLLCSYYKFREHNKGYYEKVRPLDLRHLGDSDILTVTPYRDQHGHRIIIYRFGLWRPNRVTVDDIFRATIVLQELGSLEPISQIMGGVAIFDLKDLGLEHILHLSPSVAQKMIALLVTSMPIRTSALHIVNQNWVFNAGFKIFKPFLNSAMRERLYIHGSDMSSLHKHIDPEHLPKRYGGLHDDYSYTLWLDMLKEQSTESNVQKDMEQLGFIFDSNAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEI6</accession>
    <name>A0AAU9FEI6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_11890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FEI6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF94196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="74" mass="8324" checksum="AFDCE28F1A090DC5" modified="2024-11-27" version="1">MQLFLYHFHQHESASSHTYVHLPLDTIDVMLWYSDSYPESTLPTPTNILRAGELALPSTPPTPSSSVSFKPHSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEJ2</accession>
    <name>A0AAU9FEJ2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">glucose-6-phosphate 1-epimerase</fullName>
            <ecNumber evidence="7">5.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_11803</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Mutarotase that catalyzes the interconversion of beta-D-galactose and alpha-D-galactose during galactose metabolism. Beta-D-galactose is metabolized in the liver into glucose 1-phosphate, the primary metabolic fuel, by the action of four enzymes that constitute the Leloir pathway: GALM, GALK1 (galactokinase), GALT (galactose-1-phosphate uridylyltransferase) and GALE (UDP-galactose-4'-epimerase). Involved in the maintenance of the equilibrium between the beta- and alpha-anomers of galactose, therefore ensuring a sufficient supply of the alpha-anomer for GALK1. Also active on D-glucose although shows a preference for galactose over glucose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>alpha-D-galactose = beta-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:28675"/>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
            <dbReference type="ChEBI" id="CHEBI:28061"/>
            <dbReference type="EC" id="5.1.3.3"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="2">
            <dbReference type="Rhea" id="RHEA:28677"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 6-phosphate = beta-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:16249"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58247"/>
            <dbReference type="EC" id="5.1.3.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the glucose-6-phosphate 1-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.15" evidence="7"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FEJ2"/>
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            <dbReference type="EMBL" id="BFF94087.1"/>
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            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="297" mass="34223" checksum="A37381C2BC8788DA" modified="2024-11-27" version="1">MATTAAAMAATSVVVLDRGNNTTCTINLHGATVVSWRVNNQEQLFVSKLASFDGKKAIRGGIPFVFPQFGPWSFGPQHGFARITRWHLERAPDRLHNGDVEAIFSLMDNEFTRSIWNYQFRITYRLILREKELHFHIGVYNPSKELSFSFNMLLHTYLKVPDVRRCQITGLHGCHFIDKTRENALYQEGREVVTVNEWTDRIYQHTPQEHVITNVVSGRKMRLHKYNFPDTVIWNPWIDRSREMSDFGDDEFPNMLCVEAGNVTSPVILLPGTAFEASQILQIIIEGNVSRKTKRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEJ4</accession>
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        <name evidence="15" type="ORF">DMAD_11779</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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    <keyword evidence="6" id="KW-0677">Repeat</keyword>
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        <location>
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            <end position="442"/>
        </location>
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        </location>
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            <end position="618"/>
        </location>
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            <begin position="37"/>
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        </location>
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        </location>
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        <location>
            <begin position="39"/>
            <end position="68"/>
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        <location>
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            <end position="121"/>
        </location>
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        </location>
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        </location>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="618" mass="68807" checksum="08BFDA297B9507F5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEK2</accession>
    <name>A0AAU9FEK2_DROMD</name>
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        <submittedName>
            <fullName evidence="17">LIM/homeobox protein Lhx3</fullName>
        </submittedName>
    </protein>
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        <name evidence="17" type="ORF">DMAD_11751</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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        </subcellularLocation>
    </comment>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="FunFam" id="1.10.10.60:FF:000219">
        <property type="entry name" value="LIM/homeobox protein Lhx3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000032">
        <property type="entry name" value="LIM/homeobox protein Lhx3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049594">
        <property type="entry name" value="Lhx3/4-like_LIM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF128">
        <property type="entry name" value="LIM3, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 12" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="40"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="100"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="174"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="176"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="295"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="351"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="361"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="392"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF94019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="433" mass="48444" checksum="1605034AB2B9050A" modified="2024-11-27" version="1">MELLKLMMFKSDFLSNGKCEDRVPPINLSQLPEFLLSTIPKCGGCHELILDRFILKVLERTWHAKCLQCSECHGQLNDKCFARNGQLFCKEDFFKRYGTKCSACDMGIPPTQVVRRAQDNVYHLQCFLCAMCSRTLNTGDEFYLMEDRKLICKRDYEEAKAKGLYLDGSLDGDQPNKRPRTTITAKQLETLKTAYNNSPKPARHVREQLSQDTGLDMRVVQVWFQNRRAKEKRLKKDAGRTRWSQYFRSMKGNCSPRTDKFLDKDELKVDYDSFSHHDLSNDSYSTVNLGLDEGASPHSIRGSYMHGSSSPSQYPPSSRSPPPVGQSHTFGSYPDNIVYTNIDHAAASTLHASKSHHRLHSSNNVSDLSNDSSPEQGYPDFPPSPDSWLGDSASTNNTSSNNNNTNNNNGGSGSGAASANVTTANPGTPSVHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FEK6</accession>
    <name>A0AAU9FEK6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Wee1-like protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_11835</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FEK6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF185">
        <property type="entry name" value="WEE1-LIKE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="55"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF94128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="388" mass="44264" checksum="14158A6A28D8DA8E" modified="2024-11-27" version="1">MTQDTAHLHVNPFIPDSLLATTQPPKAEDVDDSMIEMCQKRKRLALDPGRLQSDFVEVKMIGSGAFGVVLHCINRLDGCAYAIKKSKVPVIGCAYEKSFLNEVWAHAVLGKHNNVVRYFSAWTEDYHLLIQYELCDGGSLQARAQTQCLGESELKIVLLHVIEGLRYIHSNNLVHMDLKPENIFLSMNPNARRLQIEEDEELNSVYGGLRPLENLVTYKIGDLGHVTSLVDPRVNEGDCRYLAKEILNGNYSNLFSADIFSLGITLFEVANRMPLPKNGQMWHSLRDGKVPYLSTLSKEFNDLMAQMMHPDPDLRPTSQSIISPSIVIKCNQHIDLELTMEKRKNEVLLNRLQKAKEQVKLFEEKEKNRMGQTEQLPAKSIFWKWFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLZ2</accession>
    <name>A0AAU9FLZ2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Formin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05202</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLZ2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014767">
        <property type="entry name" value="DAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="77"/>
            <end position="581"/>
        </location>
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        <location>
            <begin position="689"/>
            <end position="1091"/>
        </location>
    </feature>
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        <location>
            <begin position="1130"/>
            <end position="1163"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
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        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
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        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
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            <begin position="572"/>
            <end position="678"/>
        </location>
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            <begin position="486"/>
            <end position="565"/>
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        <location>
            <begin position="1068"/>
            <end position="1106"/>
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            <begin position="70"/>
            <end position="79"/>
        </location>
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        <location>
            <begin position="243"/>
            <end position="257"/>
        </location>
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        <location>
            <begin position="572"/>
            <end position="583"/>
        </location>
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            <begin position="604"/>
            <end position="637"/>
        </location>
    </feature>
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        <location>
            <begin position="638"/>
            <end position="648"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00023449"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51231"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF96603.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1177" mass="133004" checksum="373BD1B148987A23" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMB1</accession>
    <name>A0AAU9FMB1_DROMD</name>
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        <recommendedName>
            <fullName evidence="3">Venom dipeptidyl peptidase 4</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="ORF">DMAD_05163</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9B family. DPPIV subfamily.</text>
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        <property type="protein sequence ID" value="BFF96545.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
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        <property type="entry name" value="IP17501P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="7">
        <location>
            <begin position="242"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="6">
        <location>
            <begin position="703"/>
            <end position="906"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="41"/>
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    </feature>
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        <location>
            <begin position="59"/>
            <end position="129"/>
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    </feature>
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        <location>
            <begin position="1"/>
            <end position="11"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="19"/>
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    </feature>
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            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="107"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010036"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00072929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF96545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="915" mass="102954" checksum="565681B317ACE495" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMB2</accession>
    <name>A0AAU9FMB2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein male-specific lethal-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_05319</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMB2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072487">
        <property type="term" value="C:MSL complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000042">
        <property type="entry name" value="male-specific lethal 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.30">
        <property type="entry name" value="MRG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008676">
        <property type="entry name" value="MRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038217">
        <property type="entry name" value="MRG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026541">
        <property type="entry name" value="MRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053820">
        <property type="entry name" value="MSL3_chromo-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880">
        <property type="entry name" value="MORTALITY FACTOR 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880:SF15">
        <property type="entry name" value="MSL COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05712">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22732">
        <property type="entry name" value="MSL3_chromo-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51640">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="MSL3 chromodomain-like" evidence="13">
        <location>
            <begin position="11"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="MRG" evidence="12">
        <location>
            <begin position="189"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="123"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00069454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22732"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF96747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="516" mass="59224" checksum="9708CB074242D911" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <name evidence="5" type="ORF">DMAD_05327</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Multiplexin collagen isoform Ap3"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MACROPHAGE RECEPTOR WITH COLLAGENOUS STRUCTURE"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Collagen_trimer"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <location>
            <begin position="481"/>
            <end position="529"/>
        </location>
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            <end position="744"/>
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        </location>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF06482"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF20010"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF96765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="787" mass="81872" checksum="913821FE054228C3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="4">EB domain-containing protein</fullName>
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    </protein>
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        <name evidence="5" type="ORF">DMAD_05502</name>
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    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMY8"/>
    <dbReference type="Proteomes" id="UP001500889">
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF01683">
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="EB" evidence="4">
        <location>
            <begin position="202"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="96"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="514"/>
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        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
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            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="329"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="353"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="524"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01683"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF97000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMZ5</accession>
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        <submittedName>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
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        </subcellularLocation>
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        <text evidence="9">Belongs to the TANC family.</text>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166:SF55">
        <property type="entry name" value="ROLLING PEBBLES, ISOFORM B"/>
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</entry>
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</entry>
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                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="CE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45985">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45985:SF1">
        <property type="entry name" value="VERMIFORM, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043728699" description="Chitin-binding type-2 domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="4">
        <location>
            <begin position="41"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="138"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF97162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="536" mass="60907" checksum="68A49DF1CC66A2E4" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FN93</accession>
    <name>A0AAU9FN93_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Iron-sulfur clusters transporter ABCB7, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP-binding cassette sub-family B member 7, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_05705</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(glutathione)4[2Fe(III)-2S] cluster(in) + ATP + H2O = (glutathione)4[2Fe(III)-2S] cluster(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67028"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167627"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67029"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FN93"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18582">
        <property type="entry name" value="ABC_6TM_ATM1_ABCB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000004">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000186">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF402">
        <property type="entry name" value="IRON-SULFUR CLUSTERS TRANSPORTER ABCB7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="121"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="446"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="682"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="687"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF97258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="703" mass="76414" checksum="D5C8B137588A32E0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUK5</accession>
    <name>A0AAU9FUK5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Tequila</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_07097</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035008">
        <property type="term" value="P:positive regulation of melanization defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160032">
        <property type="term" value="P:Toll receptor ligand protein activation cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000015">
        <property type="entry name" value="Atrial natriuretic peptide-converting enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.140.10:FF:000003">
        <property type="entry name" value="Tequila, isoform D"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000026">
        <property type="entry name" value="Tequila, isoform D"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <begin position="1898"/>
            <end position="2172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="195"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="403"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="455"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="758"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1520"/>
            <end position="1574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="287"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="297"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="411"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="462"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="758"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="841"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="918"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="1320"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="1339"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="1629"/>
            <end position="1644"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="1667"/>
            <end position="1682"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="2186"/>
            <end position="2204"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="2313"/>
            <end position="2328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF98990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2487" mass="277165" checksum="E07931CABCDA36E4" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUL4</accession>
    <name>A0AAU9FUL4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD repeat domain phosphoinositide-interacting protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_07407</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Autophagy-related 18a, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="392"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="420"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF99518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="454" mass="48142" checksum="D2314B681B9EE778" modified="2024-11-27" version="1">MMSLLGRPDVDAGEVFVNFNQNITSLAVATSGGYSLYSLGSVDSTLDKIYHTKSDELFLIERLFESSLVAIVSQRAPRKLKVCHFKKQSEICNYSYANTILAVKLNRERLIVCLEESLYIHNIQDMKVVHTIRDTPCNPLGLCALSSSSEHCYLAYPGSVTSGEVQIFDAINLHAKTMIPAHDTPLAAIAFSPSGTEIATASERGTVIRVFSSQDGSRLFELRRGLKRCVSIVSLSFSNCSEYLVSSSNTETVHIFRLDRSAAETADHGKQSTDDWMGYSFFRFLSKTVTSYLPTQVTDVFSQGRAFASVSLPEAGVRRMCAIATIQKQLRLLIASQDGYLYVYSIPSIEGAECQLIKRHDLRLEDHYAMDIKGLNSGVSIGGAAGVPPSVAGAGAGASGSADGKSTATDKPGGSSSSTSGGGGGGASGSGSAGASYASAVKGDEPVAGGPSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUM5</accession>
    <name>A0AAU9FUM5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein SCAI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_07313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUM5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022709">
        <property type="entry name" value="SCAI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016607">
        <property type="entry name" value="SCAI_metazoan/Viridiplantae"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21243">
        <property type="entry name" value="PROTEIN SCAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12070">
        <property type="entry name" value="SCAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF013022">
        <property type="entry name" value="UCP013022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF99397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="594" mass="68359" checksum="4AB975E544A92D0B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUM6</accession>
    <name>A0AAU9FUM6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_07375</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUM6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14081">
        <property type="entry name" value="STKc_BRSK1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14340">
        <property type="entry name" value="UBA_BRSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000064">
        <property type="entry name" value="BR serine/threonine-protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048622">
        <property type="entry name" value="BRSK1_2-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF99327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2237" mass="242696" checksum="B44329E0FCAF70AC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUN5</accession>
    <name>A0AAU9FUN5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Trichohyalin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_07156</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUN5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050801">
        <property type="entry name" value="Ca-Dep_Lectins_ImmuneDev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22801:SF63">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22801">
        <property type="entry name" value="LITHOSTATHINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043773402" evidence="2">
        <location>
            <begin position="25"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="71"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="607"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="740"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="576"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="743"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF99196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="769" mass="93132" checksum="2FB1FB43975A9C0C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUN7</accession>
    <name>A0AAU9FUN7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_07617</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. PAR subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUN7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14695">
        <property type="entry name" value="bZIP_HLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000007">
        <property type="entry name" value="hepatic leukemia factor isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF27">
        <property type="entry name" value="GH27708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="517"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="272"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="449"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="549"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="85"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="274"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="455"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF99799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="583" mass="64491" checksum="AC57CA878EA1F4B8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUN8</accession>
    <name>A0AAU9FUN8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">JNK-interacting protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein sunday driver</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07625</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK-signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Syd is required for efficient kinesin-I mediated axonal transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Forms homo- and heterooligomeric complexes. Binds the TPR motif-containing C-terminal of kinesin light chain, Klc. Pre-assembled syd scaffolding complexes are then transported as a cargo of kinesin, to the required subcellular location.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JIP scaffold family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUN8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008432">
        <property type="term" value="F:JUN kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:MAP-kinase scaffold activity"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUR7</accession>
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        <name evidence="13" type="ORF">DMAD_07625</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Arthropoda</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13 14">RF1</strain>
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        <text evidence="5">The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK-signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Syd is required for efficient kinesin-I mediated axonal transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Forms homo- and heterooligomeric complexes. Binds the TPR motif-containing C-terminal of kinesin light chain, Klc. Pre-assembled syd scaffolding complexes are then transported as a cargo of kinesin, to the required subcellular location.</text>
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        <text evidence="2">Belongs to the JIP scaffold family.</text>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
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        <name evidence="5" type="ORF">DMAD_07646</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
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        <property type="entry name" value="Ankyrin-SOCS_Box"/>
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    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24193:SF121">
        <property type="entry name" value="ADA2A-CONTAINING COMPLEX COMPONENT 3, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24193">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="89"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="122"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="155"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="529"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF99851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="586" mass="64408" checksum="D81B8E5829154117" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV38</accession>
    <name>A0AAU9FV38_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Alpha-ketoglutarate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_07241</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="5"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FV38"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1150:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000007">
        <property type="entry name" value="2-oxoglutarate dehydrogenase e1 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11610:FF:000003">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="18">
        <location>
            <begin position="645"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFF99306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1009" mass="112557" checksum="CFB7C4A0F7995229" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVI1</accession>
    <name>A0AAU9FVI1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Serine-rich adhesin for platelets</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_00198</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Rough endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030867">
        <property type="term" value="C:rough endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023041">
        <property type="term" value="P:neuronal signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019130">
        <property type="entry name" value="Macoilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13289:SF3">
        <property type="entry name" value="BIFOCAL, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13289">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1-BINDING PROTEIN BIFOCAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="367"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="541"/>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVI6</accession>
    <name>A0AAU9FVI6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Signal recognition particle 54 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_07479</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 15">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The M domain binds the 7SL RNA in presence of SRP19 and binds the signal sequence of presecretory proteins.</text>
    </comment>
    <comment type="domain">
        <text evidence="15">The NG domain, also named G domain, is a special guanosine triphosphatase (GTPase) domain, which binds GTP and forms a guanosine 5'-triphosphate (GTP)-dependent complex with a homologous NG domain in the SRP receptor subunit SRPRA. The two NG domains undergo cooperative rearrangements upon their assembly, which culminate in the reciprocal activation of the GTPase activity of one another. SRP receptor compaction upon binding with cargo-loaded SRP and GTPase rearrangement drive SRP-mediated cotranslational protein translocation into the ER.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FVI6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.30:FF:000002">
        <property type="entry name" value="Signal recognition particle 54 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000022">
        <property type="entry name" value="Signal recognition particle 54 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006325">
        <property type="entry name" value="SRP54_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01425">
        <property type="entry name" value="SRP54_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 15" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11 15" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="16">
        <location>
            <begin position="239"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU364034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF99624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="478" mass="52674" checksum="C98BCB793FFC2637" modified="2024-11-27" version="1">MLKEICAALLEADVNIRLVKQLRENVRAVIDFEEMAGGLNKRRMIQSAVFKELIKLVDPGVKPYQPIKGKANIIMFVGLQGSGKTTTCTKLAYHYQKRNWKSCLVCADTFRAGAYDQIKQNATKARIPFYGSYTEIDPVVIAQEGVDMFKREGFEMIIVDTSGRHKQEESLFEEMLAVANAVNPDNIIFVMDATIGQACEAQAKAFKDKVDIGSVIITKLDGHAKGGGALSAVAATQSPIIFIGTGEHIDDLEPFKTKPFVSKLLGMGDIEGLIDKVNELKLDGNEELLEKIKHGHFTIRDMYEQFQNIMKMGPFSQIMNMIPGFSQDFMTKGGEAESMARIKRMMTMMDSMSDNELDNRDGAKLFSKQPTRCIRVAQGAGVLEREVKELIAHYTKFAAVVKKMGGVKGLFKQGDMTKNVNPTQMAKLNQQMAKMIDPRMLQQMGGVGGLQNMMRQLQQGAAGGLGGLGNLMGGFGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVJ5</accession>
    <name>A0AAU9FVJ5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_00223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FVJ5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG00167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="345" mass="37807" checksum="0530AEE6C517A15A" modified="2024-11-27" version="1">MSFGNIGNTSDLECDKNEYPRQRPTGIVTKVAAHKLSSEPKWADKRDDDSDSEVSSPDNFLTKGAFLLTPSYELSMERAALICEKMSFKGCFSLTKTATGILFKFSHPDDYQAVFKKGFHKVTGARFYRKQSFSRSIAIPCRPRKTFTLYVLDVPEDLPVEDVRHAMYKFDSVVEVVRLHIYSSPSSSSSSSNVNANASSAAAAAAAAAASAEKSAEGEQTLRRAAALRSGSKILGSLSGSVVGDAIDKAERPERRELPPAVIRITLASMDEYNVLLQNGLNFYDATFFPTEANISLKGARIDYKRRMLDGSIPGRVRELLPVFDAAGFCKLPPPTSKLIKPPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVK5</accession>
    <name>A0AAU9FVK5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nitrilase and fragile histidine triad fusion protein NitFhit</fullName>
            <ecNumber evidence="3">3.6.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_07637</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Cleaves A-5'-PPP-5'A to yield AMP and ADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>P(1),P(3)-bis(5'-adenosyl) triphosphate + H2O = AMP + ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13893"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the N-terminal section; belongs to the UPF0012 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.29" evidence="3"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FVK5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047710">
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        <property type="entry name" value="Fragile histidine triad"/>
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        <property type="entry name" value="nitrilase homolog 1 isoform X1"/>
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    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
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        <location>
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            <position position="390"/>
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    <evidence type="ECO:0000256" key="1">
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    <sequence length="422" mass="47792" checksum="04236F13AB094DCA" modified="2024-11-27" version="1">MRSTNDKVGNLSQVKELIKKAKSENAQMIFLPECCDFVGETRAQTIELAERLDGKLVSEYKELAKCHHMWLSLGGIHELNDQGKIYNAHVLISEKGELASVYRKMHLFDATTKDIRLRESDTVSAGERLEKPVETAAGQVGLQICYDLRFAEPAILLRKLGAQLLTYPAAFTYATGKAHWDILLRARAIETQCFVVAAAQQGWHNQKRQSWGHSMIISPWGKVLADCGGEKDLDIATANIDLSILDSLRQSMPCFDHRRNDLYSLTAYNIRGREPDVDRAFADNIVDKRTIFYESEHCFAFTNLRCVVEGHVLVSTKRVTPRLCGLNCAEMTDMFATVCMVQRLLEKIYQTTSATVTVQDGAQAGQTVPHVHFHVMPRRQGDFGHNDQIYVKLEDCTESKPPRTLQERINEAQVYRTYFNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVK6</accession>
    <name>A0AAU9FVK6_DROMD</name>
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        <alternativeName>
            <fullName evidence="13">Neural tissue-specific F-actin-binding protein I</fullName>
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        <alternativeName>
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        </alternativeName>
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        <name evidence="20" type="ORF">DMAD_07260</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="20 21">RF1</strain>
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    <keyword evidence="9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
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        <location>
            <begin position="1304"/>
            <end position="1392"/>
        </location>
    </feature>
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        <location>
            <begin position="1782"/>
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        <location>
            <begin position="1505"/>
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            <dbReference type="ARBA" id="ARBA00082439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
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            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="BFF99335.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1874" mass="204801" checksum="ADF83138B505686C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVL0</accession>
    <name>A0AAU9FVL0_DROMD</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ORF">DMAD_00226</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
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        <property type="protein sequence ID" value="BFG00175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Rho_GTPase_activation_prot"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="140"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="1256"/>
            <end position="1449"/>
        </location>
    </feature>
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            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
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        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50238"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG00175.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2245" mass="236549" checksum="7D832D8AAB50DD1E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FVL4</accession>
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        <recommendedName>
            <fullName evidence="17">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Drosophila madeirensis</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="14">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="project" value="InterPro"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <location evidence="1">Cilium basal body</location>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="4" type="ORF">DMAD_03187</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6L3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF229">
        <property type="entry name" value="CUTICULAR PROTEIN 49AF, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CUTICLE"/>
    </dbReference>
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        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043426201" evidence="3">
        <location>
            <begin position="18"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG04148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="127" mass="14065" checksum="8E1783F558EAF954" modified="2024-11-27" version="1">MRTLLLCAALFVVAAWAADTSDLISQESNVEYNGKYHYHYELADGSKATQDGLLKTVSAEHDGEAVHGKYSFVSDDGQTYVVSYTADENGYHAVGDHLPTPPPTPLSVLRTLEYLKLHPYTPPERQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6L7</accession>
    <name>A0AAU9G6L7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_02931</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6L7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Narrow, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043762252" description="C-type lectin domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="26"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="362"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG03754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="503" mass="55028" checksum="A1BA8CB4ED5707D0" modified="2024-11-27" version="1">MAKIILFCVLSLLACAAGQRITTIHLDGVQYFISRMNPYSPELNYFLAYQYCRSLGLQLASFETKEKAESMTTYLKNAGYGNYDFWTSGNRLGTGMFLWMSTGLPFNATFDFFENSADAIQAGLLDPVDHNSNTSPQRTARDSSGAEKGCVILKQPTLKWMPEDCSAVKDFICEQTRCYYYNYGSIPVSSAQGRPITSTTPRPPAALMHLHAATTTTPLPLLMSTTGGLYTAAKAKSSPAIAHRLAEEASSPSFHSFKLNQDRSLTDKDAIDVEVDDQDADMDAEEHDEHEHEVESDGDVEGDDHEGDSDNFSEHARELVGDGEGDDIKEHVFPLHDNELHPEMHSIEEVQQQLQHHDEDAAPAPAAAAAAIAAAAEESVSAEHEPEADGDSEPEAEAEAETDSEAESQAPGQASEALPSSTESPAAAAIEERIKQIAQDFQKMASSQEMQTPSNEENLPKQSLSLNDLIRTLRPNEQQIIPQIDSDYSNAMRVLGKTLVPHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6M1</accession>
    <name>A0AAU9G6M1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Muskelin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_03200</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6M1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000018">
        <property type="entry name" value="Muskelin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000066">
        <property type="entry name" value="Muskelin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000148">
        <property type="entry name" value="Muskelin, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052456">
        <property type="entry name" value="CTLH_complex_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010565">
        <property type="entry name" value="Muskelin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15526">
        <property type="entry name" value="MUSKELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15526:SF5">
        <property type="entry name" value="MUSKELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06588">
        <property type="entry name" value="Muskelin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CTLH" evidence="4">
        <location>
            <begin position="241"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="792"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="796"/>
            <end position="809"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFG04163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="854" mass="97931" checksum="8732FA2910F6419D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6N3</accession>
    <name>A0AAU9G6N3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Patronin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_02937</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The CKK domain binds microtubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CAMSAP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036449">
        <property type="term" value="C:microtubule minus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030507">
        <property type="term" value="F:spectrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.360:FF:000002">
        <property type="entry name" value="Patronin, isoform M"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <end position="203"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="268"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG04165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="353" mass="41406" checksum="A22AC45E4F1541CF" modified="2024-11-27" version="1">MECCLSEEAKEQKRINQEIEKQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGYIKLVFQNIFMAMQSMIKAMDMLKISYGVGEHSELADLVMSIDYETVTTFEDPFLNAIKTLWADAGIQECYDRRREYQLTDSAKYYLSDLERIELPDFLPTEQDILRARVPTTGILEYPFDLDGIVFRMVDVGGQRSERRKWIHCFENVTSIIFLVALSEYDQILFESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAIAAREFILRMFVDLNPDSEKIIYSHFTCATDTENIRFVFAAVKDTILQSNLKEYNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6N8</accession>
    <name>A0AAU9G6N8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Paired amphipathic helix protein Sin3a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Histone deacetylase complex subunit Sin3a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Transcriptional corepressor Sin3a</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_03204</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression. Required for cortical neuron differentiation and callosal axon elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with ARID4B, BRMS1L, HCFC1, HDAC1, HDAC2, MXI1, SAP30L, SAP130, SFPQ and TOPORS. Interacts with OGT (via TPRs 1-6); the interaction mediates transcriptional repression in parallel with histone deacetylase. Interacts with BAZ2A, MXD1, MXD3, MXD4, MBD2, DACH1, NCOR1, NR4A2, REST, RLIM, SAP30, SETDB1, SMYD2, and SUDS3. Interacts with PHF12 in a complex composed of HDAC1, PHF12 and SAP30. Interacts with TET1; the interaction recruits SIN3A to gene promoters. The large PER complex involved in the histone deacetylation is composed of at least HDAC1, PER2, SFPQ and SIN3A. Interacts with KLF11. Interacts with PPHLN1. Found in a complex with YY1, GON4L and HDAC1. Interacts (via PAH2) with FOXK1. Interacts with FOXK2. Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1. Interacts with SINHCAF. Interacts with SPHK2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000001">
        <property type="entry name" value="Paired amphipathic helix protein Sin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000002">
        <property type="entry name" value="Paired amphipathic helix protein SIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000004">
        <property type="entry name" value="Paired amphipathic helix protein Sin3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.11">
        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013194">
        <property type="entry name" value="HDAC_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF0">
        <property type="entry name" value="SIN3A, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00761">
        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="20">
        <location>
            <begin position="1124"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="218"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="711"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="883"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1087"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1400"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1471"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1524"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1819"/>
            <end position="1863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1894"/>
            <end position="1943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="2115"/>
            <end position="2141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="235"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="266"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="721"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1088"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1099"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1414"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1428"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1441"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1526"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1540"/>
            <end position="1556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="1836"/>
            <end position="1850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1894"/>
            <end position="1904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1911"/>
            <end position="1937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="2122"/>
            <end position="2138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="BFG04178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2170" mass="229086" checksum="2BBF1FE5D0B4F357" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6R8</accession>
    <name>A0AAU9G6R8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific protein-tyrosine kinase</fullName>
            <ecNumber evidence="1">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_02558</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="1"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03260.1"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1179" mass="127065" checksum="12CDBE67A70EC18C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G739</accession>
    <name>A0AAU9G739_DROMD</name>
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        <submittedName>
            <fullName evidence="11">Zinc finger protein 135</fullName>
        </submittedName>
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        <name evidence="11" type="ORF">DMAD_02919</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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        </lineage>
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    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_C2H2_sf"/>
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    </dbReference>
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        <property type="entry name" value="RIKEN CDNA 6720489N17 GENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
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        <property type="match status" value="6"/>
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    </dbReference>
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        <location>
            <begin position="283"/>
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        <location>
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        <location>
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        <location>
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        <location>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="823" mass="89209" checksum="B4C78A03DCB48D2C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7I0</accession>
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        <recommendedName>
            <fullName evidence="9">Syndecan</fullName>
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    </protein>
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        <name evidence="14" type="ORF">DMAD_02904</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
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        <text evidence="9">Cell surface proteoglycan.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
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        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G7I0"/>
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        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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            <begin position="86"/>
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        </location>
    </feature>
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            <begin position="118"/>
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        <location>
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            <begin position="260"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000649"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00294"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="432" mass="45202" checksum="FA11D3594B2C6968" modified="2024-11-27" version="1">MMPKQRTSTREETLSLRSNGKFLMLSALLMGLLVAATQAVDQKSVKATPAEPSALSGGAASQAASAPRDAIYIDDDSIEGSGGRGGIHDDLEKDPDYSGSGFGPDDEDAEPDHHSQHGSHNTRISQSGGSSINTHTPTQTSSTTSTTTSSSPTTTSSSSTTTSTSTTTAATTTSTTAASNRTQTTPKPLVVDSTTASRPLPHFPVFDEELDVDEDASGDGIDVEGGGEDDDEGDDDDIIIDHPDSEKPKFAVPVPAGSDNVDHDHDHDIDSDTDDGDGDNDDTDDDDRIIDVDGGNEDGTTNGNGNGIGGNDGDLNTERGPGGGSNVHELDPNTNVNSQPSDTKPVDHRPNGNEVVIMSEDDRTSSFFSQPGILAAVIGGAVVGLLCAILVVMFIVYRMRKKDEGSYALDEPKRSPANNSYAKNANNREFYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7I1</accession>
    <name>A0AAU9G7I1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Myocyte-specific enhancer factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_03459</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G7I1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000001">
        <property type="entry name" value="Myocyte-specific enhancer factor 2A homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HJURP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF534">
        <property type="entry name" value="MYOCYTE-SPECIFIC ENHANCER FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12347">
        <property type="entry name" value="HJURP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="11">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="170"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="333"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="503"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="232"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="442"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="503"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG04508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="550" mass="58977" checksum="032E52D9BC789349" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7I2</accession>
    <name>A0AAU9G7I2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Longitudinals lacking protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_03127</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Putative transcription factor required for axon growth and guidance in the central and peripheral nervous systems. Repels CNS axons away from the midline by promoting the expression of the midline repellent sli and its receptor robo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G7I2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008406">
        <property type="term" value="P:gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035167">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007526">
        <property type="term" value="P:larval somatic muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045476">
        <property type="term" value="P:nurse cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007464">
        <property type="term" value="P:R3/R4 cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045467">
        <property type="term" value="P:R7 cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000091">
        <property type="entry name" value="Lola, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF111">
        <property type="entry name" value="LONGITUDINALS LACKING PROTEIN, ISOFORMS F_I_K_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="11">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="548"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="577"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="115"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="159"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="230"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="352"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="402"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="462"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="165"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="264"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="462"/>
            <end position="471"/>
        </location>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG04079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="607" mass="65004" checksum="BD56919D2CB49E3C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7I5</accession>
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        <submittedName>
            <fullName evidence="15">Neogenin</fullName>
        </submittedName>
    </protein>
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        <name evidence="15" type="ORF">DMAD_02711</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
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    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. DCC family.</text>
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        <property type="protein sequence ID" value="BFG03461.1"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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        <property type="match status" value="3"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
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        <location>
            <begin position="27"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1074"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
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            <begin position="23"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
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        </location>
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    <feature type="domain" description="Ig-like" evidence="13">
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            <end position="422"/>
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        <location>
            <begin position="459"/>
            <end position="552"/>
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    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="558"/>
            <end position="648"/>
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    <feature type="domain" description="Fibronectin type-III" evidence="14">
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            <end position="746"/>
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        </location>
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    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="955"/>
            <end position="1056"/>
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        <location>
            <begin position="1223"/>
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            <begin position="1227"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00009588"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1378" mass="151857" checksum="365902BEBD764145" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7I6</accession>
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        <recommendedName>
            <fullName evidence="3">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">DMAD_03011</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="94"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="425"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="731"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="783"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="812"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="851"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="435"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="464"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="488"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="691"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="823"/>
            <end position="834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG03885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="999" mass="109656" checksum="D0482FF0778B028E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7I8</accession>
    <name>A0AAU9G7I8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Myocyte-specific enhancer factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_03459</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
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        <property type="entry name" value="MEF2-like_N"/>
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    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF534">
        <property type="entry name" value="MYOCYTE-SPECIFIC ENHANCER FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12347">
        <property type="entry name" value="HJURP_C"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SRF-TF"/>
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        <property type="entry name" value="MADSDOMAIN"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="MADS_BOX_2"/>
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    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
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            <begin position="1"/>
            <end position="61"/>
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            <begin position="170"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="333"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="442"/>
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        <location>
            <begin position="170"/>
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        <location>
            <begin position="207"/>
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        <location>
            <begin position="232"/>
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        <location>
            <begin position="363"/>
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        <location>
            <begin position="442"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="478"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG04510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="527" mass="56215" checksum="EFBE17EAFF997D06" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7I9</accession>
    <name>A0AAU9G7I9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">MAP/microtubule affinity-regulating kinase 3</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Serine/threonine-protein kinase par-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">DMAD_02833</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Regulates localization and activity of MITF by mediating its phosphorylation, promoting subsequent interaction between MITF and 14-3-3 and retention in the cytosol. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1. Phosphorylates PKP2 and KSR1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with MAPT/TAU. Interacts with DLG5 (via coiled-coil domain). Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5. Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ. Interacts with PKP2 (via N-terminus). Interacts with CDC25C. Interacts with KSR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
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    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G7I9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12196">
        <property type="entry name" value="MARK1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14072">
        <property type="entry name" value="STKc_MARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14337">
        <property type="entry name" value="UBA_MARK_Par1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001032">
        <property type="entry name" value="KP78b, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000001">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049508">
        <property type="entry name" value="MARK1-4_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02149">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 27" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="485"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="25">
        <location>
            <begin position="755"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="26">
        <location>
            <begin position="1118"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="71"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="287"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="361"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="800"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="918"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="997"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="313"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="397"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="808"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="845"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="921"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="954"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1010"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="514"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00054424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00063680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00074935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="BFG03627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1167" mass="127452" checksum="B4960C987EE4FB36" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7J0</accession>
    <name>A0AAU9G7J0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">MAP/microtubule affinity-regulating kinase 3</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Serine/threonine-protein kinase par-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">DMAD_02833</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Regulates localization and activity of MITF by mediating its phosphorylation, promoting subsequent interaction between MITF and 14-3-3 and retention in the cytosol. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1. Phosphorylates PKP2 and KSR1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with MAPT/TAU. Interacts with DLG5 (via coiled-coil domain). Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5. Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ. Interacts with PKP2 (via N-terminus). Interacts with CDC25C. Interacts with KSR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
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    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G7J0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12196">
        <property type="entry name" value="MARK1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14072">
        <property type="entry name" value="STKc_MARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14337">
        <property type="entry name" value="UBA_MARK_Par1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001032">
        <property type="entry name" value="KP78b, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000001">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049508">
        <property type="entry name" value="MARK1-4_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02149">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
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        <source>
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    </evidence>
    <sequence length="1163" mass="127105" checksum="5912D013BF82AC37" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
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</entry>
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</entry>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="289" mass="32661" checksum="D3DA813D2E63823B" modified="2024-11-27" version="1">MLDTKWMLLLLWHLWSLWSTSAFDYCDPALCPGPERHIACNNFGELADSCSPDAHVVRISPNRRNMILNELNEYRDRIARGDLLGFSPATRMATLQWDPELASFAELNVKRCALVNDHCRNSEQFRNVAQVVAEGGWQGAPERPVPGSTTDHTVEYHTEDEVIKATLEQMFAEYKECTMRDIIAYSPPISSKCIAYFTQLVRDSTTHVGCGILRESRNTSNEAGQWLLSVHQYMTCNFVRASDVNAPVYQSGDRSATECRSGRNPMFINLCSINELYDTSSIMQGLGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G7L2</accession>
    <name>A0AAU9G7L2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pupal cuticle protein 20</fullName>
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    </protein>
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        <name evidence="5" type="ORF">DMAD_03487</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
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        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
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        <property type="entry name" value="CUTICLE"/>
    </dbReference>
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        <property type="entry name" value="CHIT_BIND_RR_1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="17"/>
        </location>
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        <location>
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            <end position="691"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="108"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="3">
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            <end position="291"/>
        </location>
    </feature>
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            <end position="413"/>
        </location>
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        </location>
    </feature>
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        <location>
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            <end position="99"/>
        </location>
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            <begin position="603"/>
            <end position="666"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <dbReference type="ChEBI" id="CHEBI:58608"/>
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        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ERL3</accession>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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            <strain evidence="2 3">RF1</strain>
        </source>
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            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF88892.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="87" mass="9754" checksum="45AF3B3575431415" modified="2024-11-27" version="1">MDVSPPRPYIRDACVSPQRLEIKQYNFETALKDYVLFHGLSTRTRTTPKCRTQTAITCTSSWTSANPKFGPVGQAGRAGLPRLQKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="1">DUF4806 domain-containing protein</fullName>
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                <person name="Yamamoto D."/>
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        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF89606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ERN1</accession>
    <name>A0AAU9ERN1_DROMD</name>
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        <recommendedName>
            <fullName evidence="3">glutamine--fructose-6-phosphate transaminase (isomerizing)</fullName>
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    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_07791</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="3"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9ERN1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000001">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="8">
        <location>
            <begin position="2"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="9">
        <location>
            <begin position="351"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="9">
        <location>
            <begin position="522"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF88907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="673" mass="75466" checksum="5BDBB2CC058B30AD" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ERS4</accession>
    <name>A0AAU9ERS4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Phosphatidylinositol-binding clathrin assembly protein LAP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_08306</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Binds clathrin and phosphatidylinositol 4,5-bisphosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PICALM/SNAP91 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9ERS4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098894">
        <property type="term" value="C:extrinsic component of presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16985">
        <property type="entry name" value="ANTH_N_AP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000017">
        <property type="entry name" value="Phosphatidylinositol-binding clathrin assembly protein LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000001">
        <property type="entry name" value="phosphatidylinositol-binding clathrin assembly protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0168">Coated pit</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="12">
        <location>
            <begin position="16"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="598"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="678"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="307"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="700"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF89583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="720" mass="76522" checksum="EF0C8386ABC79851" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ES24</accession>
    <name>A0AAU9ES24_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Tyramine/octopamine receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_07885</name>
    </gene>
    <organism evidence="15 16">
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            <begin position="40"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F3L9</accession>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Brachycera</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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    <dbReference type="FunFam" id="3.30.420.40:FF:000028">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000003">
        <property type="entry name" value="heat shock cognate 71 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="305"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00072095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF89360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="339" mass="36572" checksum="A6807A2301E5A784" modified="2024-11-27" version="1">MDPVEKALRDAKLDKSAIHDIVLVGGSTRIPKVQRLLQDLFNGKELNKSINPDEAVAYGAAVQAAILHGDKSQEVQDLLLLDVTPLSLGIETAGGVMSVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVFEGERAMTKDNNLLGKFELSGIPPAPRGVPQIEVTFDIDANGILNVTALERSTNKENKITITNDKGRLSKEDIERMVNEAEKYRNEDEKQKETISAKNALESYCFNMKATLDEDNLKTKIADSDRTVILDKCNETIKWLDANQLADKEEYEHRQKELEGVCNPIITKLYQSAGGAPGGMPGGMPGAPGAAGAGAPGAGTGGAGPTIEEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F436</accession>
    <name>A0AAU9F436_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Phosphatidylinositol-binding clathrin assembly protein LAP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_08306</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Binds clathrin and phosphatidylinositol 4,5-bisphosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PICALM/SNAP91 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F436"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098894">
        <property type="term" value="C:extrinsic component of presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16985">
        <property type="entry name" value="ANTH_N_AP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000017">
        <property type="entry name" value="Phosphatidylinositol-binding clathrin assembly protein LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000001">
        <property type="entry name" value="phosphatidylinositol-binding clathrin assembly protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0168">Coated pit</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="12">
        <location>
            <begin position="16"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="609"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="689"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="711"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF89585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="731" mass="77738" checksum="BE52A311AB2CC8ED" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F437</accession>
    <name>A0AAU9F437_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glucose dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_07667</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F437"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF216">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE OXIDOREDUCTASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043526894" evidence="5">
        <location>
            <begin position="21"/>
            <end position="581"/>
        </location>
    </feature>
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        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
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    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="512"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="556"/>
        </location>
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    <feature type="binding site" evidence="3">
        <location>
            <begin position="58"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
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        <location>
            <position position="134"/>
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        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
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        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000137-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF88741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="581" mass="65403" checksum="4016E56D97B1644E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDN7</accession>
    <name>A0AAU9FDN7_DROMD</name>
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        <recommendedName>
            <fullName evidence="2">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">DMAD_11601</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDN7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="CDD" id="cd16081">
        <property type="entry name" value="TSPcc_insect"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="von Willebrand factor D and EGF domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024665">
        <property type="entry name" value="TSP/COMP_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10199">
        <property type="entry name" value="THROMBOSPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10199:SF100">
        <property type="entry name" value="THROMBOSPONDIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11598">
        <property type="entry name" value="COMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043863220" description="EGF-like domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="301"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="342"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF93829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="411" mass="46587" checksum="F7F2F59C45BF56EE" modified="2024-11-27" version="1">MNWTRVLLIGLTALALTFVEVASLSLDPVASAELEQFIRKGDVVISTRHIRPRRKLHISIEALFMIDFPMLKHKMSFFLDRKQQRVTLDISANGATESRNFEIPNINETSTIRSLALQFSKNRITLHVDCKPSTHHDIDMNLAKLYTQMDDPVIKLFRERKYPLHFDADMEHSLQRANCQKGLHRRGNRRMLRNKITEREKNKKRDVRGWYEPTISREGVTDHRHQEVPTDVERGDIPILHGDCEDALARSLSDLLALVKLLREDVAHQRQEIAYLRMLLENCAGCKNPLSGDNQLRLEPTCRTANPCYRGVDCLDSAAGPRCGRCPAGLIGDGKICKPGVTCANFQCYPGVQCHDTVNGAQCDSCPVGYEGDGRTCSLRNPCMDTPCPSGNIHYQRQSQYTARKYSVITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDP0</accession>
    <name>A0AAU9FDP0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="18">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_11572</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 18">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="18"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDP0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07541">
        <property type="entry name" value="P-type_ATPase_APLT_Neo1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000097">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000009">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF5">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 17 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 18" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="892"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="918"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="970"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="999"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1031"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1054"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="21">
        <location>
            <begin position="56"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="20">
        <location>
            <begin position="153"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="22">
        <location>
            <begin position="858"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="442"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="496"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="442"/>
            <end position="458"/>
        </location>
    </feature>
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        <location>
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</entry>
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</entry>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">RF1</strain>
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    </reference>
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            <end position="120"/>
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    </feature>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDS0</accession>
    <name>A0AAU9FDS0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ethanolamine-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CTP:phosphoethanolamine cytidylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_11596</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.14" evidence="10"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDS0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004306">
        <property type="term" value="F:ethanolamine-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02173">
        <property type="entry name" value="ECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044608">
        <property type="entry name" value="Ect1/PCYT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780:SF2">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="13">
        <location>
            <begin position="40"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Cytidyltransferase-like" evidence="13">
        <location>
            <begin position="226"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF93820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="390" mass="43574" checksum="C0233244D60C9E1C" modified="2024-11-27" version="1">MSDKQVNGCSSAKSYSSPCNGNTTQKQEESPINGKDVRVWCDGCYDMVHFGHANSLRQAKALGDKVIVGIHTDAEITKHKGPPVFTEEERVKMVKGIKWVDEVVLGAPYVTTLEVLDQNNCDFCVHGDDITMTAEGVDTYHLVKSANRYKEVRRTAGVSTTDLVGRMLLLTRNHFRQGSAEYDIEKEDSAAKSPWTGCSQFLPTTQKIIQFSDGKSPNAGDKIVYVAGAFDLFHVGHLDFLEKASKLGDYLIVGLHTDPVVNSYKGSNYPIMNLHERVLSVLACKFVNEVVIGAPYCVTEELLEHFKIDVVCHGRTPIAMEDGKIDPYAVPKTKAIFELIDSGNEMTTERIVERIISHRLEYERRNQAKEKKEIEAFEALQRQKQTQKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDT7</accession>
    <name>A0AAU9FDT7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translocation protein SEC62</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_11685</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC62 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDT7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004728">
        <property type="entry name" value="Sec62"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443:SF9">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03839">
        <property type="entry name" value="Sec62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="216"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="115"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="280"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="330"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="355"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="397"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF93941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="408" mass="46167" checksum="9C312F160A646686" modified="2024-11-27" version="1">MSEKKRTRRRKDEYAEPGQQKVDKPSKDEKNVAKWLKKNVKTKKTKFLSHVVEYFTSCKALDALMKSKFAEGNNPLFTTREQAVEFMDVMLEHKFFHRAKKVPVTLDEIRGKSAEKKGEIKEKEKPKDDKKDTDLEADSGNGDGPSATAEKEKKEKKKRKIRLDMHPEQIFVDGSEAYVWIYDPIPLHYWIFGFILLLGAVGICLFPLWPPLLRKGVYYLSIAAAGFLVFILALTVVRLIVFTIVWALTGGKLHFWIFPNLTEDVGFFASFWPLYESNYGSDAASASSKTDKKSKLKSKKKEKDSDAEDTAVDADGDADGDVDAEVSTLEPEKIELIKEHDADMEVRKRRKVGADEYEDDDVDVDEEPQSQPKESKAGTPQNSESDSETSSKDYEIISSSEVQNVAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDU3</accession>
    <name>A0AAU9FDU3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Liprin-alpha-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_11606</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the liprin family. Liprin-alpha subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDU3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048786">
        <property type="term" value="C:presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09562">
        <property type="entry name" value="SAM_liprin-alpha1_2_3_4_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09565">
        <property type="entry name" value="SAM_liprin-alpha1_2_3_4_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09568">
        <property type="entry name" value="SAM_liprin-alpha1_2_3_4_repeat3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000002">
        <property type="entry name" value="PTPRF interacting protein alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000004">
        <property type="entry name" value="PTPRF interacting protein alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057892">
        <property type="entry name" value="LIP-1_CC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037620">
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        <property type="entry name" value="LAR INTERACTING PROTEIN LIP -RELATED PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587:SF20">
        <property type="entry name" value="LIPRIN-ALPHA, ISOFORM E"/>
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    <dbReference type="Pfam" id="PF25526">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="3"/>
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        <property type="entry name" value="SAM_DOMAIN"/>
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            <begin position="895"/>
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            <dbReference type="PROSITE" id="PS50105"/>
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            <dbReference type="EMBL" id="BFF93838.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDU5</accession>
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        <submittedName>
            <fullName evidence="5">Protein Aatf</fullName>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5 6">RF1</strain>
        </source>
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        <text evidence="1">Belongs to the AATF family.</text>
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        <property type="protein sequence ID" value="BFF93832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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            <begin position="181"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13339"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="BFF93832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="460" mass="52748" checksum="0A23EBAF635BE86D" modified="2024-11-27" version="1">MLRKSKKEPQTVADKVSKLLAHPNGSDSEEDSDFDVATVPRVVDFEQDEYDLPDARSTDIRKRNVKLLSEQSERYKGKISSRKELEEEEDQSEDEVAYEDSDSDEDDALASFNQKLKAGASKLADDEESSGDEDDDDDEAALAVFNQKLKSGSEEEDDEDVDDEEEDDDDDDEEDDDDEEEIQKGLCVQNQLRIWERLLELRMNTQKVTSKANQLPPPETLKSLGSENEELQSVLSEATERSSKLLRQLLALQTALSQQNSETRKSVKRKALPSEEIEPPLKVFASVLQNNFQNMTSYRNEVLLKWDDRTKLLTPGAGLKRKSLQEDYDIIKKIDSALANREALVERSQTLKSNQPEQENDPPGQRQPHVYDDSDFYHQQLRELIEYKASTSSNMSEITKQFVELQKLRQKMKKKVDTRASKGRKLRYVVHNKLTNFMAPNEACEWTESSKSELYKSLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDU7</accession>
    <name>A0AAU9FDU7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Sodium/potassium/calcium exchanger Nckx30C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_11634</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
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        <property type="protein sequence ID" value="BFF93873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:intracellular calcium ion homeostasis"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000004">
        <property type="entry name" value="Sodium/potassium/calcium exchanger 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00367">
        <property type="entry name" value="calcium/sodium antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF72">
        <property type="entry name" value="SODIUM_POTASSIUM_CALCIUM EXCHANGER NCKX30C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0769">Symport</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="410"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="445"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
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            <begin position="681"/>
            <end position="705"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="717"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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            <begin position="813"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="16">
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            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="16">
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="14">
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="14">
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            <begin position="284"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
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            <begin position="508"/>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="508"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF93873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="841" mass="92045" checksum="6B6FA18F6FBA5DA1" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDU9</accession>
    <name>A0AAU9FDU9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SBF1/SBF2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_11614</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDU9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039872">
        <property type="entry name" value="KIAA0513"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022096">
        <property type="entry name" value="SBF1/SBF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13663">
        <property type="entry name" value="SIMILAR TO RIKEN CDNA 6430548M08"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13663:SF2">
        <property type="entry name" value="SIMILAR TO RIKEN CDNA 6430548M08"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12335">
        <property type="entry name" value="SBF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBF1/SBF2" evidence="2">
        <location>
            <begin position="367"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="496"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="521"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF93850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="619" mass="68336" checksum="50CF0B0BF09D6CD1" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FE01</accession>
    <name>A0AAU9FE01_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Neuronal acetylcholine receptor subunit alpha-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_11650</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FE01"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18997">
        <property type="entry name" value="LGIC_ECD_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000058">
        <property type="entry name" value="Nicotinic acetylcholine receptor alpha6, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000059">
        <property type="entry name" value="Nicotinic acetylcholine receptor alpha6, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000016">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043085012" evidence="17">
        <location>
            <begin position="27"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="468"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="18">
        <location>
            <begin position="30"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="19">
        <location>
            <begin position="244"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF93898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="494" mass="56139" checksum="BDF76B6EF5C44017" modified="2024-11-27" version="1">MDSSLPSSLSLFVLLIFLAIIKESCQGPHEKRLLNHLLSTYNTLERPVANESEPLEVKFGLTLQQIIDVDEKNQLLITNLWLSLEWNDYNLRWNETEYGGVKDLRITPNKLWKPDVLMYNSADEGFDGTYHTNIVVKHNGSCLYVPPGIFKSTCKIDITWFPFDDQHCEMKFGSWTYDGNQLDLVLNSEDGGDLSDFITNGEWYLLAMPGKKNTIVYACCPEPYVDITFTIQIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDSGEKLTLGVTILLSLTVFLNLVAESMPTTSDAVPLIGTYFNCIMFMVASSVVLTVVVLNYHHRTADIHEMPPWIKSVFLQWLPWILRMGRPGRKITRKTILLSNRMKELELKERSSKSLLANVLDIDDDFRHTISGSQTAIGSSASFGRPTTVEEHHTAIGCNHKDLHLILKELQFITSRMRKADDEAELISDWKFAAMVVDRFCLIVFTLFTIIATVTVLLSAPHIIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FE03</accession>
    <name>A0AAU9FE03_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Sodium/potassium/calcium exchanger Nckx30C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_11634</name>
    </gene>
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                <person name="Llopart A."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF93876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="868" mass="94521" checksum="2CF30F0B7A3F5396" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FE24</accession>
    <name>A0AAU9FE24_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_11687</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FE24"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031935">
        <property type="entry name" value="DUF4770"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031936">
        <property type="entry name" value="DUF4771"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41967">
        <property type="entry name" value="FI19406P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41967:SF6">
        <property type="entry name" value="FI19406P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15994">
        <property type="entry name" value="DUF4770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15995">
        <property type="entry name" value="DUF4771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4770" evidence="3">
        <location>
            <begin position="53"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4771" evidence="4">
        <location>
            <begin position="579"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="151"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="405"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="410"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF93945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="763" mass="87356" checksum="E004EEDD94051861" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FE26</accession>
    <name>A0AAU9FE26_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Acetylcholine receptor subunit alpha-type acr-16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_11650</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FE26"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18997">
        <property type="entry name" value="LGIC_ECD_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000058">
        <property type="entry name" value="Nicotinic acetylcholine receptor alpha6, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000059">
        <property type="entry name" value="Nicotinic acetylcholine receptor alpha6, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000016">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043094297" evidence="17">
        <location>
            <begin position="27"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="468"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="18">
        <location>
            <begin position="30"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="19">
        <location>
            <begin position="244"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF93897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="494" mass="56174" checksum="03CEEC55CC125185" modified="2024-11-27" version="1">MDSSLPSSLSLFVLLIFLAIIKESCQGPHEKRLLNHLLSTYNTLERPVANESEPLEVKFGLTLQQIIDVDEKNQLLITNLWLSLEWNDYNLRWNETEYGGVKDLRITPNKLWKPDVLMYNSADEGFDGTYHTNIVVKHNGSCLYVPPGIFKSTCKIDITWFPFDDQHCEMKFGSWTYDGNQLDLVLNSEDGGDLSDFITNGEWYLLAMPGKKNTIVYACCPEPYVDITFTIQIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDSGEKLTLGVTILLSLTVFLNLVAETLPQVSDAIPLLGTYFNCIMFMVASSVVLTVVVLNYHHRTADIHEMPPWIKSVFLQWLPWILRMGRPGRKITRKTILLSNRMKELELKERSSKSLLANVLDIDDDFRHTISGSQTAIGSSASFGRPTTVEEHHTAIGCNHKDLHLILKELQFITSRMRKADDEAELISDWKFAAMVVDRFCLIVFTLFTIIATVTVLLSAPHIIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FE27</accession>
    <name>A0AAU9FE27_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytokine-like nuclear factor N-PAC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Glyoxylate reductase 1 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Nuclear protein NP60 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Putative oxidoreductase GLYR1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_11684</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Binds to mononucleosomes. Interacts with male-specific lethal (MSL) histone acetyltransferase complex at least composed of mof, msl-1, msl-2 and msl-3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HIBADH-related family. NP60 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FE27"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05836">
        <property type="entry name" value="PWWP_GLYR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000039">
        <property type="entry name" value="Blast:Putative oxidoreductase GLYR1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000058">
        <property type="entry name" value="Putative oxidoreductase GLYR1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035501">
        <property type="entry name" value="GLYR1_PWWP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051265">
        <property type="entry name" value="HIBADH-related_NP60_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580:SF2">
        <property type="entry name" value="CYTOKINE-LIKE NUCLEAR FACTOR N-PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580">
        <property type="entry name" value="OXIDOREDUCTASE GLYR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="10">
        <location>
            <begin position="22"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="175"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="180"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00078412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00082969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF93940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="616" mass="66485" checksum="3DCCC0685E7B87F0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FE29</accession>
    <name>A0AAU9FE29_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 184C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_11678</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FE29"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="368"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="398"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF93932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="500" mass="57064" checksum="923AD01F6941281A" modified="2024-11-27" version="1">MCRLDIRHFCEDWRIWIRPLLIVTYGIFAIIVVPLLIVNSVKDGFQRNDQLILIGGLFVLSAVPVSIWHIIQHVIHFTKPILQKHIIRILWMVPIYALNAWIGLFFPKHSIYVDSFRECYEAYVIYNFMVYLLNYLNLNMDLEITMEYKPQVNHFFPLCCMRPWIMGREFIHNCKHGILQYTVVRPITTFIAVICELCGVYGEGEFAGNVAFPYIVVVNNISQFVAMYCLVLFYRANKEDLKPMKPIPKFLCIKAVVFFSFFQGVLLNVLVYYGIIKDIFGSDVGDTNLASMLQNFLICIEMFVAAVAHIYSFPHHPFHINSPQYWNNPNHSWCRAFLSMMDISDMQEDVTEHLGVVGSTLSRRFQGRSTYQPLARGPRRSSSESEYLISKRQDQQLSGGSSTQSGAAGAGDSSNSNNYQQQQLHLPGARSQPSPRQRETQHLRERERERERDQQQQHFVGAGGSSFLRLVPVAGAASSAAPIPESNDDYALLLGAGAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FE54</accession>
    <name>A0AAU9FE54_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Gamma-interferon-inducible lysosomal thiol reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_11714</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GILT family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FE54"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016671">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004911">
        <property type="entry name" value="Interferon-induced_GILT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234">
        <property type="entry name" value="GAMMA-INTERFERON INDUCIBLE LYSOSOMAL THIOL REDUCTASE GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234:SF71">
        <property type="entry name" value="GAMMA-INTERFERON-INDUCIBLE LYSOSOMAL THIOL REDUCTASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03227">
        <property type="entry name" value="GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF93975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="182" mass="20416" checksum="6510C57023691509" modified="2024-11-27" version="1">MVPTRKRIFIIICIVVILGCLLRILLFNSPLPPVTDQTNYQRAIGAPVLVMVFYEALCPDSKYFITKQLLTAYEVAAPIMEVVSCMIRDNRLPQEAMRKCVKQYSENIDLVQKCYDSDHGLELMKHNGEATHSLRPQVTFIPTITIDGSQGRQASILKNLLSEVCKAAGDTDQAKKICKNTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FE59</accession>
    <name>A0AAU9FE59_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein gustavus</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_11703</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPSB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FE59"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12906">
        <property type="entry name" value="SPRY_SOCS1-2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000007">
        <property type="entry name" value="SPRY domain-containing SOCS box protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050672">
        <property type="entry name" value="FBXO45-Fsn/SPSB_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245:SF11">
        <property type="entry name" value="PROTEIN GUSTAVUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245">
        <property type="entry name" value="SPRY DOMAIN CONTAINING SOCS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="5">
        <location>
            <begin position="106"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="6">
        <location>
            <begin position="293"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF93962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="349" mass="39823" checksum="60CB259B25AF3996" modified="2024-11-27" version="1">MELGKKRYKGGRTPSIRSSERRRPQSISSLSTLSPRVVLSRLEPRDLERLRLSYKVAIDQRRSRSCRGMNMGQKISGGVKTVSRNDSQATFKPIIPRELQADFLKPARIDILLDMPPAIRELQLKHSWNSEDRSLNIFVKEDDKLTFHRHPVAQSTDCIRGKVGLTKGLHIWEIYWPTRQRGTHAVVGVSTADAPLHSVGYQSLVGSTEQSWGWDLGRNKLYHDSKNCAGVTYPAILKNDEAFLVPDKFLVALDMDEGTLSFIVDQQYLGIAFRGLKGKKLYPVVSAVWGHCEITMRYIGGLDPEPLPLMDLCRRTIRQKIGRANLEERIQQLQLPLSMKTYLLYKNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMA0</accession>
    <name>A0AAU9FMA0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_05347</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMA0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="191"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="145"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="372"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="719"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="386"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="399"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="412"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF96789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="742" mass="79392" checksum="BFF0FB26CB2CF756" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMA1</accession>
    <name>A0AAU9FMA1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Neurexin-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_05334</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the neurexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMA1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000242">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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                <person name="Yamamoto D."/>
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        <source>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Yamamoto D."/>
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        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
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    <dbReference type="Pfam" id="PF10744">
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="830"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="879"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
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    <feature type="compositionally biased region" description="Pro residues" evidence="10">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="16"/>
            <end position="81"/>
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            <begin position="152"/>
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            <begin position="152"/>
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        </location>
    </feature>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF96862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="249" mass="26922" checksum="27BBB2449C2EE141" modified="2024-11-27" version="1">MSGGMDKRKLSTSGDSLYEILGLPKTATGDDIKKTYRKLALKYHPDKNPDNVDASDKFKEVNRAHSILSDQTKRNIYDNYGSLGLYIAEQFGEENVNAYFVVTSPAVKAVFICCAVVTGCCCCCCCCCCCNFCCGKFKPPVNESHDQYAHLNRPDGNREASDLPPHLGHQPRLEDVDLDDVNLGAGGGPVTSQPREQPGGQPVFAMPPPTGAAVGVNPFTGAPVAANENTSLNTTEQTTYTPDMVNQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUP0</accession>
    <name>A0AAU9FUP0_DROMD</name>
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        <submittedName>
            <fullName evidence="5">rRNA 2'-O-methyltransferase fibrillarin</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="ORF">DMAD_07026</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RAM family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RNA GUANINE-N7 METHYLTRANSFERASE ACTIVATING SUBUNIT-RELATED"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="50"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="79"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF99020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="168" mass="18914" checksum="4A4CABB57D598CCB" modified="2024-11-27" version="1">MVDPNRKINRLPEKDHFLEECEAEFSSRYTEDDDEFMAHCSKPVPGPPIVENWMSGGGGGGGGGGGGGYNNRYNGNRRGGGDRGWQRREGGYQHNNHDRGGGYRDNHNRNRNRYDSYQRRDRGGHHHGEGGGGGQKRSFDQRPSRNDRDQPPIKVRRDYGNFVPASKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUP2</accession>
    <name>A0AAU9FUP2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tight junction-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_07424</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUP2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043441">
        <property type="entry name" value="Tjap1/BEGAIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28664">
        <property type="entry name" value="TIGHT JUNCTION-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28664:SF4">
        <property type="entry name" value="TIGHT JUNCTION-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="132"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="684"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="19"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="137"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="684"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="729"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF99548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="939" mass="100936" checksum="9470F8B83BBDD838" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUP4</accession>
    <name>A0AAU9FUP4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neurabin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Neurabin-I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Neural tissue-specific F-actin-binding protein I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Protein phosphatase 1 regulatory subunit 9A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_07260</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06790">
        <property type="entry name" value="PDZ_neurabin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09512">
        <property type="entry name" value="SAM_Neurabin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000010">
        <property type="entry name" value="Neurabin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000008">
        <property type="entry name" value="Neurabin-1 isoform 1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040645">
        <property type="entry name" value="Neurabin-1/2_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043446">
        <property type="entry name" value="Neurabin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16154">
        <property type="entry name" value="NEURABIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16154:SF6">
        <property type="entry name" value="SPINOPHILIN, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="440"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="459"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="493"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="509"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="975"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="992"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1057"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1434"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1703"/>
            <end position="1719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1745"/>
            <end position="1761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1919"/>
            <end position="1932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1985"/>
            <end position="1997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="2036"/>
            <end position="2056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="2067"/>
            <end position="2092"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF99339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2205" mass="239367" checksum="58878C7088EBA6C8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUP7</accession>
    <name>A0AAU9FUP7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">JNK-interacting protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein sunday driver</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07625</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK-signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Syd is required for efficient kinesin-I mediated axonal transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Forms homo- and heterooligomeric complexes. Binds the TPR motif-containing C-terminal of kinesin light chain, Klc. Pre-assembled syd scaffolding complexes are then transported as a cargo of kinesin, to the required subcellular location.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JIP scaffold family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUP7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008432">
        <property type="term" value="F:JUN kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030159">
        <property type="term" value="F:signaling receptor complex adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1000:FF:000001">
        <property type="entry name" value="C-Jun-amino-terminal kinase-interacting protein 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1000">
        <property type="entry name" value="arf6 gtpase in complex with a specific effector, jip4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039911">
        <property type="entry name" value="JIP3/JIP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032486">
        <property type="entry name" value="JIP_LZII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034743">
        <property type="entry name" value="RH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034744">
        <property type="entry name" value="RH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13886:SF4">
        <property type="entry name" value="JNK-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13886">
        <property type="entry name" value="JNK/SAPK-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16471">
        <property type="entry name" value="JIP_LZII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09744">
        <property type="entry name" value="RH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19056">
        <property type="entry name" value="WD40_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51776">
        <property type="entry name" value="RH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51777">
        <property type="entry name" value="RH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RH1" evidence="11">
        <location>
            <begin position="18"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="RH2" evidence="12">
        <location>
            <begin position="443"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="271"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="507"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="801"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="857"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="79"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="377"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="516"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="531"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="862"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00059054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00082388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF99814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1216" mass="135471" checksum="C7BD812B97342A22" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUQ1</accession>
    <name>A0AAU9FUQ1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="22">AFG3-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_07183</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUQ1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000003">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1690.20:FF:000001">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF2">
        <property type="entry name" value="AFG3 LIKE MATRIX AAA PEPTIDASE SUBUNIT 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="21">
        <location>
            <begin position="353"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="70"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="771"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="BFF99228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="818" mass="89446" checksum="2B9619D5EFD90250" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUQ2</accession>
    <name>A0AAU9FUQ2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glycerol kinase</fullName>
            <ecNumber evidence="3">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07636</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="3"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUQ2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000108">
        <property type="entry name" value="Glycerol kinase, glycosomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF82">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6 10 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="11">
        <location>
            <begin position="2"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="12">
        <location>
            <begin position="103"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF99836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="355" mass="38779" checksum="D1DF7771E2A7DFE1" modified="2024-11-27" version="1">MFGTIDTWLMYNLTGGKNGGVHKTDVTNASRTMLMNIETLQWDPNLLKFFGLPKTILPEICSSAEFYGRIEQGVLKGTSVTSVLGDQQAALVGQQCLSKGQAKATYGTGCFLLYNTGASIVHSQHGLLTTVGYQLGRKATPYYALEGSVSIAGAAFNWLRDNIGIIQNSGQIEAMAATVNNSLDVYFVPAFNGLYAPYWNQDARGVICGLSEETTSEHIVRATLEAVCFQVRDILDSMHKDCKIPLARLMVDGGMTVNNLFLQLQSDLVGIHVLRAKIAETTALGAAMAAYKAVENRYSMEAPLSKSGPREPIKPTIGSTERNLRYQKWKMAIDRSLNWETSTLPQGEREAFDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUQ4</accession>
    <name>A0AAU9FUQ4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger protein on ecdysone puffs</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_07399</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUQ4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026811">
        <property type="entry name" value="CIZ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056345">
        <property type="entry name" value="Znf-C2H2_CIZ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
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        <property type="entry name" value="Znf_C2H2_type"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15491:SF9">
        <property type="entry name" value="CIP1-INTERACTING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23330">
        <property type="entry name" value="zf-C2H2_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="3"/>
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    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="470"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="85"/>
            <end position="152"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="166"/>
            <end position="192"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="331"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="513"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="123"/>
            <end position="138"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="187"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="331"/>
            <end position="343"/>
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        <location>
            <begin position="370"/>
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            <begin position="386"/>
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        <location>
            <begin position="628"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="704"/>
            <end position="713"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF99508.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="713" mass="76867" checksum="3BDD565F6CFB5039" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUQ6</accession>
    <name>A0AAU9FUQ6_DROMD</name>
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        <submittedName>
            <fullName evidence="18">Spectrin beta chain non-erythrocytic 5</fullName>
        </submittedName>
    </protein>
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        <name evidence="18" type="ORF">DMAD_07437</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the spectrin family.</text>
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        <property type="component" value="Chromosome J"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
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    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="CH_beta_spectrin_rpt1"/>
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    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
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    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0728">SH3 domain</keyword>
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        <location>
            <begin position="80"/>
            <end position="183"/>
        </location>
    </feature>
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        <location>
            <begin position="887"/>
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    </feature>
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        <location>
            <begin position="3803"/>
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        </location>
    </feature>
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        <location>
            <begin position="39"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="707"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="3652"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="3757"/>
            <end position="3802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="3923"/>
            <end position="4188"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="527"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="2022"/>
            <end position="2049"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="2227"/>
            <end position="2254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="2551"/>
            <end position="2578"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="2972"/>
            <end position="2999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="723"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="3652"/>
            <end position="3693"/>
        </location>
    </feature>
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        <location>
            <begin position="3757"/>
            <end position="3767"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="3783"/>
            <end position="3793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="3929"/>
            <end position="3953"/>
        </location>
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        <location>
            <begin position="3957"/>
            <end position="3970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="3980"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="4017"/>
            <end position="4038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="4054"/>
            <end position="4063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="4074"/>
            <end position="4089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="4097"/>
            <end position="4120"/>
        </location>
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        <location>
            <begin position="4121"/>
            <end position="4153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="4169"/>
            <end position="4181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006826"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFF99563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="4188" mass="481371" checksum="1ED71767CC891406" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUQ8</accession>
    <name>A0AAU9FUQ8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Serine hydrolase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_07039</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SERINE HYDROLASE-LIKE PROTEIN DDB_G0286239"/>
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    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
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        <location>
            <begin position="33"/>
            <end position="289"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF99035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="330" mass="37320" checksum="E640F2D6ECF9FF2A" modified="2024-11-27" version="1">MGTLSLDDFKAVKIPVPWGHISGRWYGNRTERPILAIHGWQDNLGTFDRLLPLLPDYLGILCIDLPGHGRSCHLQPGMHYSVDDYVDIIPRVMKEYGWQKVSLLGHSMGAILMFVFAALAPHAVDMVISLDILISECRQPEGNNLRLAAHNMEKHLGEDERLVGGCSREPPAYPLQKMCSALAKGSFNSVRQEVAHHMLHRQVTRSRLYPGKYYFSRDGRIKFINHAYLDNGLFAEMARRIETNPYLVIKASDSPYVGPSEALSILTANNPNFEFHEVQGTHHVHLNEPEKCAQYIVPFLIRHRPPPTLNSWSKEQSLLGTKVFHASSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUU9</accession>
    <name>A0AAU9FUU9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine protease 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_07173</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUU9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="serine proteases 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF99216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="132" mass="14256" checksum="7893F1628670DBCA" modified="2024-11-27" version="1">MVNKVELPSYNDRYNDYNEWWAVACGWGGTYDGSPLPDWLQCADLQIIHNSECASYYGTGIVGDNIICVRVVDGKGTCGGDSGGPLVSHDGGKLVGVTNWVSGAGCQAGHPAGFQRVTYHLDWIRDHTGISY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUV7</accession>
    <name>A0AAU9FUV7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Spectrin beta chain non-erythrocytic 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_07437</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the spectrin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUV8</accession>
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        <submittedName>
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            <taxon>Insecta</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="match status" value="4"/>
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        <property type="entry name" value="EGF"/>
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        <property type="entry name" value="EGF-like_CS"/>
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        <property type="entry name" value="LE_dom"/>
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        <property type="entry name" value="MEGF10/SREC1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24043:SF8">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24043">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="659"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="43"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="256"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="342"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="430"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="518"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="799"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="850"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="799"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="867"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="63"/>
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    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="281"/>
            <end position="290"/>
        </location>
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    <feature type="disulfide bond" evidence="5">
        <location>
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            <end position="376"/>
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    <feature type="disulfide bond" evidence="5">
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            <begin position="455"/>
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        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF99239.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="887" mass="96905" checksum="24C46E50B8FBC45E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV05</accession>
    <name>A0AAU9FV05_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Purple acid phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_00055</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.</text>
    </comment>
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    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFF99934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Purple acid phosphatase-like, N-terminal"/>
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    </dbReference>
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        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR041792">
        <property type="entry name" value="MPP_PAP"/>
    </dbReference>
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        <property type="entry name" value="PAPs_C"/>
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        <property type="entry name" value="PAPs_N"/>
    </dbReference>
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        <property type="entry name" value="Purple_acid_Pase-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45867:SF3">
        <property type="entry name" value="ACID PHOSPHATASE TYPE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45867">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14008">
        <property type="entry name" value="Metallophos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16656">
        <property type="entry name" value="Pur_ac_phosph_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49363">
        <property type="entry name" value="Purple acid phosphatase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043110776" description="Purple acid phosphatase" evidence="3">
        <location>
            <begin position="28"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase N-terminal" evidence="6">
        <location>
            <begin position="40"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="4">
        <location>
            <begin position="145"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase C-terminal" evidence="5">
        <location>
            <begin position="373"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF99934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="450" mass="51737" checksum="CCA23E596F682DFD" modified="2024-11-27" version="1">MEPLHQLLMLSSLLLVAGICFMPQVLAAEQDKVQIVHYQPEQVHLAFGESVLEMVVTWNTRDNTNESICEFGIEGLQQLAKSPHGPTAFVDGGAKKATQYIHRVTLTKLEANSTYRYHCGSQLGWSATYWFRTQFSHSDWSPSLAIYGDMGVVNAASLPALQRETQRGLYDAVIHVGDFAYDMCNENGEVGDEFMRQVETVAAYVPYMVCVGNHEEKYNFSHYTNRFSMPGGSENMFYSFNMGPVHFIGFSTEFYYFTQYGLGQIVMQYDWLERDLIEANRPENRQKRPWIITFGHRPMYCSNANSDDCTNHETVVRKGLPFLDMFGLEPLFYKYGVDVELWAHEHCYERMWPMYNYTVYNGSMAEPYVNPGAPIHIISGAAGNHEGREPFLERMPPWSAFHSQDFGYLRLQAHNRSHLYFEQVSDDKKGEVIDHFWVVKDKHGAYGSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV06</accession>
    <name>A0AAU9FV06_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase</fullName>
            <ecNumber evidence="6">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_00041</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 9">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="8">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="6"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFF99917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FV06"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042592">
        <property type="term" value="P:homeostatic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16212">
        <property type="entry name" value="EFh_NorpA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13361">
        <property type="entry name" value="PH_PLC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08591">
        <property type="entry name" value="PI-PLCc_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000024">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1230.10:FF:000005">
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <location>
            <begin position="1924"/>
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        <location>
            <begin position="1996"/>
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        <location>
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    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="358"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="424"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="440"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="459"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="493"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="509"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="975"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="992"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1057"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1439"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1714"/>
            <end position="1730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1756"/>
            <end position="1772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1930"/>
            <end position="1943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1996"/>
            <end position="2008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="2047"/>
            <end position="2067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="2078"/>
            <end position="2103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00067399"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00076637"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00077125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00082439"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF99333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2216" mass="240506" checksum="A0BB3D45B85A9804" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV15</accession>
    <name>A0AAU9FV15_DROMD</name>
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        <submittedName>
            <fullName evidence="6">WD repeat domain phosphoinositide-interacting protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_07407</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:autophagy"/>
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    </dbReference>
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        <property type="entry name" value="Autophagy-related 18a, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROPPIN"/>
    </dbReference>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
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    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="387"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="415"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF99517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="449" mass="47441" checksum="9C1157337FB43C18" modified="2024-11-27" version="1">MMSLLGRPDVDAGEVFVNFNQNITSLAVATSGGYSLYSLGSVDSTLDKIYHTKSDELFLIERLFESSLVAIVSQRAPRKLKVCHFKKQSEICNYSYANTILAVKLNRERLIVCLEESLYIHNIQDMKVVHTIRDTPCNPLGLCALSSSSEHCYLAYPGSVTSGEVQIFDAINLHAKTMIPAHDTPLAAIAFSPSGTEIATASERGTVIRVFSSQDGSRLFELRRGLKRCVSIVSLSFSNCSEYLVSSSNTETVHIFRLDRSAAETADHGKQSTDDWMGFLSKTVTSYLPTQVTDVFSQGRAFASVSLPEAGVRRMCAIATIQKQLRLLIASQDGYLYVYSIPSIEGAECQLIKRHDLRLEDHYAMDIKGLNSGVSIGGAAGVPPSVAGAGAGASGSADGKSTATDKPGGSSSSTSGGGGGGASGSGSAGASYASAVKGDEPVAGGPSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV22</accession>
    <name>A0AAU9FV22_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Kelch-like protein diablo</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_07370</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. May have a role in synapse differentiation and growth.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FV22"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18459">
        <property type="entry name" value="BACK_KLHL20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18249">
        <property type="entry name" value="BTB_POZ_KLHL20_KLEIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000006">
        <property type="entry name" value="Kelch-like family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000001">
        <property type="entry name" value="Kelch-like family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF451">
        <property type="entry name" value="KELCH-LIKE PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="9">
        <location>
            <begin position="74"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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</entry>
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            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Ephydroidea</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:neuron projection development"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="1399"/>
        </location>
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        <location>
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        <location>
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        </location>
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        </location>
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        <location>
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        </location>
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        <location>
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        <location>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV26</accession>
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        </lineage>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">RF1</strain>
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        <source>
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        <source>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFF99964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FV32"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002031">
        <property type="term" value="P:G protein-coupled receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.840:FF:000004">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000698">
        <property type="entry name" value="Arrestin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014753">
        <property type="entry name" value="Arrestin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11792">
        <property type="entry name" value="ARRESTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11792:SF18">
        <property type="entry name" value="FI20035P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00309">
        <property type="entry name" value="ARRESTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="3">
        <location>
            <begin position="301"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="166"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="438"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="525"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="647"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="438"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="548"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="567"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF99964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="694" mass="75150" checksum="55CB2E98D3243798" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV33</accession>
    <name>A0AAU9FV33_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_07496</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06608">
        <property type="entry name" value="STKc_myosinIII_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000003">
        <property type="entry name" value="TRAF2 and NCK-interacting protein kinase isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000006">
        <property type="entry name" value="TRAF2 and NCK-interacting protein kinase isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051700">
        <property type="entry name" value="STE20_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096:SF1">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="34"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="13">
        <location>
            <begin position="1303"/>
            <end position="1591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="317"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="371"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="453"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="759"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="902"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="975"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1048"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1169"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="373"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="453"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="495"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="510"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="521"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="566"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="579"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="589"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="647"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="768"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="800"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="847"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="903"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="975"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="985"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1048"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1061"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1088"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1121"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1131"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF99643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1617" mass="173303" checksum="30234C3549DD23AF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV57</accession>
    <name>A0AAU9FV57_DROMD</name>
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        <recommendedName>
            <fullName evidence="17">PH-sensitive chloride channel 1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="15" type="ORF">DMAD_07374</name>
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    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="BFF99477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Neur_channel"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
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        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
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    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="668"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="704"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1018"/>
            <end position="1039"/>
        </location>
    </feature>
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        <location>
            <begin position="449"/>
            <end position="634"/>
        </location>
    </feature>
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        <location>
            <begin position="647"/>
            <end position="752"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
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        <location>
            <begin position="128"/>
            <end position="168"/>
        </location>
    </feature>
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        <location>
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            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="785"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="841"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="922"/>
            <end position="947"/>
        </location>
    </feature>
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        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
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        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
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        <location>
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            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="878"/>
            <end position="887"/>
        </location>
    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023303"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF99477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1043" mass="115824" checksum="87FF6EC2412BF194" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV58</accession>
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        </recommendedName>
        <alternativeName>
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        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Neural tissue-specific F-actin-binding protein I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Protein phosphatase 1 regulatory subunit 9A</fullName>
        </alternativeName>
    </protein>
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        <name evidence="20" type="ORF">DMAD_07260</name>
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    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <subcellularLocation>
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        </subcellularLocation>
    </comment>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
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        <property type="match status" value="1"/>
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    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
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    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1311"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="18">
        <location>
            <begin position="2140"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="81"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="193"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="646"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="982"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1062"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
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    <sequence length="2232" mass="242098" checksum="6963999790F0D1AA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV64</accession>
    <name>A0AAU9FV64_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dynamin</fullName>
            <ecNumber evidence="2">3.6.5.5</ecNumber>
        </recommendedName>
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        <name evidence="18" type="ORF">DMAD_00115</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
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        </reaction>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
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    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
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        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <begin position="787"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00048040"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00067339"/>
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        <source>
            <dbReference type="RuleBase" id="RU003932"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
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        <source>
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</entry>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <end position="1392"/>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50106"/>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV66</accession>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Tsg C-terminal" evidence="8">
        <location>
            <begin position="87"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF99992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="258" mass="29281" checksum="3ACACCA57B1D7CE9" modified="2024-11-27" version="1">MDIWRSLTVGAFLLLGLVCFYGHVESCNEVVCASIVSKCMLTQSCKCELKNCSCCKECLKCLGKKYYEECCSCVELCPKPNDTRNSLSKKSHVEDFDGVPELFNVLVTTPDDSDTFGYNWNVFTFQVDFDKYLKGPKLEKDGHYYLRTNDKNLDEAIQDRDNIVTVNCTVIYLDQCVSWNKCRKSCQTTGASSTRWFHDGCCECVGSTCINYGVNESRCRNCPESKDLGDDFDENMEEEMQDFGDNMGPFDGSVNNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV70</accession>
    <name>A0AAU9FV70_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein bric-a-brac 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_07441</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probably acts as a transcriptional regulator. Required for the specification of the tarsal segment. Also involved in antenna development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FV70"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003680">
        <property type="term" value="F:minor groove of adenine-thymine-rich DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007455">
        <property type="term" value="P:eye-antennal disc morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046660">
        <property type="term" value="P:female sex differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007478">
        <property type="term" value="P:leg disc morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000120">
        <property type="entry name" value="Bric a brac 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007889">
        <property type="entry name" value="HTH_Psq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF109">
        <property type="entry name" value="FI07618P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05225">
        <property type="entry name" value="HTH_psq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50960">
        <property type="entry name" value="HTH_PSQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BTB" evidence="9">
        <location>
            <begin position="225"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="HTH psq-type" evidence="10">
        <location>
            <begin position="695"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="7">
        <location>
            <begin position="723"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="314"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="365"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="592"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="855"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="946"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="403"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="439"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="500"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="524"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="859"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="946"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="957"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="968"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="987"/>
            <end position="1019"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF99577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1132" mass="120415" checksum="BE07783DCB241EBF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV72</accession>
    <name>A0AAU9FV72_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA-binding protein D-ETS-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_07309</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FV72"/>
    <dbReference type="SMR" id="A0AAU9FV72"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000343">
        <property type="entry name" value="Ets at 65A, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF304">
        <property type="entry name" value="DNA-BINDING PROTEIN D-ETS-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ETS" evidence="7">
        <location>
            <begin position="84"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="45"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF99393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="257" mass="28701" checksum="3B8075A12BE46679" modified="2024-11-27" version="1">MLDIKSSADYLSRSTGGFSNFSMLFADSSYKSSWGSHSSTQSQGYSSNALSIKHDPHSQLRQPDPYQMFGPTSSRLASSGSGQIQLWQFLLELLSDSNNASCITWEGTNGEFKLTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFQGLAAATQPAASDPTYKYQSDLFMTPYHHSAKLSSFMSPHHGMTSSSASIFPSAASWGNWGSPATNLYQPHSMSHVTPSHVAPHLSSYPHYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV77</accession>
    <name>A0AAU9FV77_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Gamma-aminobutyric acid receptor subunit beta-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_00111</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Postsynaptic cell membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FV77"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099095">
        <property type="term" value="F:ligand-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19006">
        <property type="entry name" value="LGIC_ECD_GABAAR_LCCH3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19049">
        <property type="entry name" value="LGIC_TM_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000040">
        <property type="entry name" value="Gamma-aminobutyric acid receptor subunit beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000074">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002289">
        <property type="entry name" value="GABAAb_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01160">
        <property type="entry name" value="GABAARBETA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0868">Chloride</keyword>
    <keyword evidence="12" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 19" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11 22" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 19" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043091575" evidence="19">
        <location>
            <begin position="30"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="250"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="315"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="20">
        <location>
            <begin position="43"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="21">
        <location>
            <begin position="257"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="BFG00008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="490" mass="55979" checksum="3B744C5744BC7805" modified="2024-11-27" version="1">MNSYTRLDVCSGLIFLILILGAKLQLARCKQNDVLAGRLENVTQTISKILQGYDIRLRPNFGGEPLHVGMDLTIASFDAISEVNMYWRDERLAFNIYGPYYNGDYNDDDGIHDVLTLSGDFAEKIWVPDTFFANDKNSFLHDVTERNKLVRLAGDGAVTYGMRFTTTLACMMDLHYYPLDSQNCTVEIESYGYTVSDVVMYWKPTPVRGVEDAELPQFTIIGYETNDRKERLATGVYQRLSLSFKLQRNIGYFVFQTYLPSILIVMLSWVSFWINHEATSARVALGITTVLTMTTISTGVRSSLPRISYVKAIDIYLVMCFVFVFAALLEYAAVNYTYWGKRAKKKIKKFKDCDRQKIGKSERSLTCSTTEDIIELQDVRMSPIPSLRKGNYNATLGSIGTETLNLAKFPPSFRITRNYGTGRNQLRHRSQRGIPTRPRMLHAIKRSASAIKATIPKIKDVNIIDKYSRMIFPISFLAFNLGYWLFYILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV84</accession>
    <name>A0AAU9FV84_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">PH-sensitive chloride channel 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_07374</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099095">
        <property type="term" value="F:ligand-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18987">
        <property type="entry name" value="LGIC_ECD_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19049">
        <property type="entry name" value="LGIC_TM_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000021">
        <property type="entry name" value="glutamate-gated chloride channel isoform X12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000020">
        <property type="entry name" value="glutamate-gated chloride channel isoform X12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="633"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="697"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="979"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="13">
        <location>
            <begin position="442"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="14">
        <location>
            <begin position="640"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="128"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="802"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="883"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="808"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="839"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF99475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1004" mass="111528" checksum="9B9B527FCFB85CFB" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6P2</accession>
    <name>A0AAU9G6P2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">MAP/microtubule affinity-regulating kinase 3</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Serine/threonine-protein kinase par-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">DMAD_02833</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Regulates localization and activity of MITF by mediating its phosphorylation, promoting subsequent interaction between MITF and 14-3-3 and retention in the cytosol. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1. Phosphorylates PKP2 and KSR1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with MAPT/TAU. Interacts with DLG5 (via coiled-coil domain). Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5. Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ. Interacts with PKP2 (via N-terminus). Interacts with CDC25C. Interacts with KSR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6P2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12196">
        <property type="entry name" value="MARK1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14072">
        <property type="entry name" value="STKc_MARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14337">
        <property type="entry name" value="UBA_MARK_Par1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001032">
        <property type="entry name" value="KP78b, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000001">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049508">
        <property type="entry name" value="MARK1-4_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02149">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 27" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="485"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="25">
        <location>
            <begin position="755"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="26">
        <location>
            <begin position="1115"/>
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        </location>
    </feature>
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    <feature type="region of interest" description="Disordered" evidence="23">
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    <feature type="region of interest" description="Disordered" evidence="23">
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    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="915"/>
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    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="994"/>
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        <location>
            <begin position="1"/>
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        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="313"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="397"/>
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        </location>
    </feature>
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        <location>
            <begin position="411"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="805"/>
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        <location>
            <begin position="842"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="918"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="951"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1007"/>
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        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="514"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00054424"/>
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</entry>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <strain evidence="3 4">RF1</strain>
        </source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
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        <text evidence="1 4 7">Belongs to the aldehyde dehydrogenase family.</text>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="526"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="10">
        <location>
            <begin position="57"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG03382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="554" mass="61497" checksum="F2D0F2C5686F4620" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6U8</accession>
    <name>A0AAU9G6U8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">GPI alpha-1,4-mannosyltransferase I, catalytic subunit</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">GPI mannosyltransferase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_02845</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 13">Catalytic subunit of the glycosylphosphatidylinositol-mannosyltransferase I complex which catalyzes the transfer of the first mannose, via an alpha-1,4 bond from a dolichol-phosphate-mannose (Dol-P-Man) to the glucosaminyl acyl phosphatidylinositol (GlcN-(acyl)PI) intermediate to generate alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol and participates in the sixth step of the glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the PIGM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6U8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990529">
        <property type="term" value="C:glycosylphosphatidylinositol-mannosyltransferase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051751">
        <property type="term" value="F:alpha-1,4-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004376">
        <property type="term" value="F:GPI mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007704">
        <property type="entry name" value="PIG-M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886:SF0">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886">
        <property type="entry name" value="PIG-M MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05007">
        <property type="entry name" value="Mannosyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 13" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="311"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFG03645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="346" mass="40338" checksum="A4720BE56FE6B1AF" modified="2024-11-27" version="1">MEIKSQYQKTVQHLLSKFNRPQEPDQSLDALDERSHPDNIARASACCWLYNPLTAVISTRGSGDCFSSFFVILTIYLLIRSDHKSSHKHWLIFAAGLAHGLVIHLRLYPLLFSLAYYLSLSSRLTRTPLDFLRQILRPNKQQFCLVLGTLLSLVAFTWTFYSLYGWQYIYEAYLYHFVRKDSRHNFSLQFLLQYLGSAATVEEPALLTKLLVVAPQFLLILYLSLSFGQFRQTLPFCIFTVAFVIVTYNSVVTSQYFIWYLAILPLCLNNFKLLSWSRSIFLLVLWLLAQALWLLPAYLLEFKTWNSFNWIGLQGAVFFVTNGYILEQLLSHYGFTQFKISYRKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6U9</accession>
    <name>A0AAU9G6U9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Myc box-dependent-interacting protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_03202</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6U9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07588">
        <property type="entry name" value="BAR_Amphiphysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11790">
        <property type="entry name" value="SH3_Amphiphysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000013">
        <property type="entry name" value="Amphiphysin isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000172">
        <property type="entry name" value="Amphiphysin, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003005">
        <property type="entry name" value="Amphiphysin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46514">
        <property type="entry name" value="AMPHIPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46514:SF3">
        <property type="entry name" value="AMPHIPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01251">
        <property type="entry name" value="AMPHIPHYSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="10">
        <location>
            <begin position="24"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="9">
        <location>
            <begin position="464"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="241"/>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15 16">RF1</strain>
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        <subcellularLocation>
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    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="5"/>
            <end position="352"/>
        </location>
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        <location>
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            <end position="1862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
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            <begin position="829"/>
            <end position="853"/>
        </location>
    </feature>
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        <location>
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        </location>
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        <location>
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        <location>
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            <end position="1477"/>
        </location>
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        <location>
            <begin position="1687"/>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
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    <feature type="binding site" evidence="10">
        <location>
            <begin position="100"/>
            <end position="107"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50245"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03305.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G703</accession>
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        <submittedName>
            <fullName evidence="2">Serine-rich adhesin for platelets</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">DMAD_02635</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
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        <location>
            <begin position="266"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="694"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="833"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1010"/>
            <end position="1035"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="730"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="838"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="885"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1012"/>
            <end position="1022"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG03350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1063" mass="115959" checksum="CBBE2B5169076146" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G705</accession>
    <name>A0AAU9G705_DROMD</name>
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        <recommendedName>
            <fullName evidence="2">polynucleotide adenylyltransferase</fullName>
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        <name evidence="6" type="ORF">DMAD_03304</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6 7">RF1</strain>
        </source>
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        <reaction evidence="4">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
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            <dbReference type="ChEBI" id="CHEBI:173115"/>
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            <dbReference type="Rhea" id="RHEA:11333"/>
        </physiologicalReaction>
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        <text evidence="1">Belongs to the TENT family.</text>
    </comment>
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    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G705"/>
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        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:mRNA stabilization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="TET5"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRION-LIKE- Q/N-RICH -DOMAIN-BEARING PROTEIN PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF1693"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="37"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="428"/>
            <end position="461"/>
        </location>
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        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00007631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFG04298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="546" mass="61120" checksum="F7C6F9D9B4B33F80" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G706</accession>
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        <submittedName>
            <fullName evidence="1">Atrophin-1</fullName>
        </submittedName>
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        <name evidence="1" type="ORF">DMAD_02726</name>
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    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G706"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFG03481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="157" mass="16607" checksum="54001F67585E92BE" modified="2024-11-27" version="1">MLAFFIALSIAFTIFVATSVVVCIRRKQKAARNVGYTINDAPVVVTSATHTAPGGYPVTQLPPPGYPAGNAYAVPYPAQTTGNVTVQMPMPMQQQHQQMPMPMPGTHTHGVAYPPYPGAGAANMNPPTYDMAVASPGPSVMPAGYEKQSPYNPNFGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G709</accession>
    <name>A0AAU9G709_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Neogenin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_02711</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. DCC family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000004">
        <property type="entry name" value="DCC isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002650">
        <property type="entry name" value="Frazzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001472">
        <property type="entry name" value="Frazzled, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001583">
        <property type="entry name" value="Frazzled, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002008">
        <property type="entry name" value="Frazzled, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001448">
        <property type="entry name" value="Frazzled, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001797">
        <property type="entry name" value="Frazzled, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000028">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010560">
        <property type="entry name" value="Neogenin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF54">
        <property type="entry name" value="FI24025P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06583">
        <property type="entry name" value="Neogenin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043840805" evidence="12">
        <location>
            <begin position="27"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1074"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="23"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="127"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="244"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="337"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="459"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="558"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="653"/>
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</entry>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G722</accession>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
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            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <begin position="1104"/>
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            <position position="46"/>
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        </source>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </location>
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            <dbReference type="Pfam" id="PF16087"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="91" mass="10204" checksum="EBD84F9FE758CBAE" modified="2024-11-27" version="1">MDQWTGVHRAFIIRAYYKSNDSISAAQRLFKKQFSIYQTPPTNVIKSWLRTFEGTGSSQAGGGTRSSLPQIFCTICNNCLSNEKNFSSSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G763</accession>
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        <submittedName>
            <fullName evidence="3">Serine-rich adhesin for platelets</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">DMAD_02635</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CDKN2A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16148">
        <property type="entry name" value="NF-KAPPA-B-REPRESSING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <end position="26"/>
        </location>
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        </location>
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        </location>
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        </location>
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        </location>
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        </location>
    </feature>
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        <location>
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            <end position="931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG03354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="972" mass="104777" checksum="F7B3F205E39E43DE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G765</accession>
    <name>A0AAU9G765_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Putative inorganic phosphate cotransporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_02758</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">May be an inorganic phosphate cotransporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sodium/anion cotransporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G765"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17318">
        <property type="entry name" value="MFS_SLC17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000144">
        <property type="entry name" value="Picot, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000003">
        <property type="entry name" value="Solute carrier family 17 member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF280">
        <property type="entry name" value="FI21844P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
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    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
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            <end position="44"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="349"/>
            <end position="371"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <end position="405"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <end position="436"/>
        </location>
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            <begin position="21"/>
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            <dbReference type="Proteomes" id="UP001500889"/>
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    <sequence length="477" mass="51638" checksum="55112B74AF065C19" modified="2024-11-27" version="1">MGFGTSLDMARPSGFGVRHWQAILLFVGMMINYFQRVDISAAIVPMTQSTAGAPSYNWNVSEKSLILSSFFWGYVVSQVPAGLLAKRFGAKIVLGTATAVGGVLSFFHPMAAASGWESVCVLRILTGLVQGTVYPCVHTLLAKWVPRTERGFLTTGIYSGAQFGTALILVTSGFIFESSLGWPGLFHLSGGLSLAWALLFFWQAANEPATARRITKTEQEYIESLTGSNSSSQSMPVPWIAIFKSPAFYGLLAGHCGFAWGFYTLLTQMPTYMNKVLHLDVKSNAFLSSLPYLAMGVLCLVVSPISDVLTNRGFISITTARKLFNSIGQWGPMACLIGLGYMTADQKTWAILLLTLAVGTNAGCSCGYLINHIDLSPNFAGPMMGVTNGIAGVTSIVAPLVVGAILTEEEDPNQWRLVFFITGAIYLVCNTLFVIFGKATVQFWNEPTSMSSTIALRNNFENDSQTIAPTTVKDNRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G768</accession>
    <name>A0AAU9G768_DROMD</name>
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        <submittedName>
            <fullName evidence="5">Heterogeneous nuclear ribonucleoprotein L</fullName>
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        <name evidence="5" type="ORF">DMAD_02818</name>
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    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <location>
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            <end position="254"/>
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            <begin position="1"/>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="413" mass="44452" checksum="7D0AAF5B2B2BA36A" modified="2024-11-27" version="1">MNNLNANATAFMWPGQPQPQPQPLPTQQPVHQAQQAQLLHGVVVPQTRKFGGNKGNYNYVQSHTAGGAMMGNGGGGGGGGGMGGGQMGPMGGNPNSYRKRQQPMNQGYNAGCFQSNEPPLLGPGAAFPPFGAPEYHPTTPDNWKGASIHPTGLMKEPTGVVPGRNAPVAFTPQGQAQGAVMMVYGLDHDTSNTDKLFNLVCLYGNVARIKFLKTKEGTAMVQMGDSVAVERCVQHLNNIPVGTGGKIQIAFSKQNFLSEVINPFLLPDHTPSFKEYTGSKNNRFLSPAQASKNRIQPPSKILHFFNTPPGLTEDQLIGIFNIKDVPATSVRLFPLKTERSSSGLIEFPNISQAVLAIMKCNHLPIEGKGTKFPFIMKLCFSSSKSMNGAWNNATNEGMIEKENEVDSKVDIYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G769</accession>
    <name>A0AAU9G769_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Patronin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_02937</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The CKK domain binds microtubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CAMSAP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036449">
        <property type="term" value="C:microtubule minus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:calmodulin binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:spectrin binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0031122">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Patronin, isoform M"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CKK domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CAMSAP"/>
    </dbReference>
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        <property type="entry name" value="CAMSAP_CC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058042">
        <property type="entry name" value="CAMSAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014797">
        <property type="entry name" value="CKK_CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038209">
        <property type="entry name" value="CKK_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595:SF0">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17095">
        <property type="entry name" value="CAMSAP_CC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08683">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25532">
        <property type="entry name" value="CH_CAMSAP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01051">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51508">
        <property type="entry name" value="CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="157"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="CKK" evidence="10">
        <location>
            <begin position="1593"/>
            <end position="1727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="334"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="413"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="813"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="884"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="939"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="984"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1131"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1185"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG03768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1746" mass="196705" checksum="AFD2E1401F2321B6" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G771</accession>
    <name>A0AAU9G771_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DENN domain-containing protein 1A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_03362</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G771"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901981">
        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.200:FF:000003">
        <property type="entry name" value="DENN domain containing 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11500:FF:000001">
        <property type="entry name" value="Putative DENN domain-containing protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040032">
        <property type="entry name" value="DENND1A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13196">
        <property type="entry name" value="DENN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13196:SF14">
        <property type="entry name" value="UDENN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03455">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="5">
        <location>
            <begin position="22"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="456"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="607"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="475"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="507"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFG04373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="781" mass="86172" checksum="44E9B5CBA543DBA3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G775</accession>
    <name>A0AAU9G775_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_02754</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM174 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G775"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009565">
        <property type="entry name" value="FAM174-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28607">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28607:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06679">
        <property type="entry name" value="DUF1180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043751001" evidence="10">
        <location>
            <begin position="22"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG03517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="200" mass="21689" checksum="0913DEB1BED7B96E" modified="2024-11-27" version="1">MYFRGSSIAATAIWLFVLVLEQQHSDVAVSAASTNGTKGINISEPSVPAAIPEALKPDDRKTVKKALSIEAVPNQKDADVVIAEKPELTLKEQPKESPQAAAPESPSSGMPVEDVRIEHSSMGNDVDFPGQAVVYVLVAITSSAILLLVLRVYRMRLTRAERKYGVQGDRANQELTPLPMAIEDVNSDEEDHTVFELVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G776</accession>
    <name>A0AAU9G776_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Diuretic hormone receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_03069</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for the insect diurectic hormone. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G776"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008036">
        <property type="term" value="F:diuretic hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15263">
        <property type="entry name" value="7tmB1_DH_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000155">
        <property type="entry name" value="diuretic hormone receptor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000023">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002001">
        <property type="entry name" value="GPCR_2_diuretic_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF15">
        <property type="entry name" value="DIURETIC HORMONE 44 RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
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    <sequence length="478" mass="54175" checksum="1D9984ECB284C79D" modified="2024-11-27" version="1">MADDDLRALVDSLDDASPENLANVIANFSLDMLQRASALIGAQQPHSGALLINRTQQCQQQSQQEEELEAATATAAAAGGKRILHCPSAFDSVLCWPRTKAATLAVLPCFEEFKGVHYDTTENATRFCFENGTWDHYSDYDRCHQQSGSVPMVPDFSPSVDLPAIIYACGYFISFATLVVALIIFVSFKDLRCLRNTIHANLFLTYITSALLWILTLFLQVITTESSQAGCITLVIMLQYFYLTNFFWMFVEGLYLYTLVVQTFSSDNISFIIYALIGWGCPAVFIMAWAIAKAFAPHLENDHFNGLEVDCGWMRESHIDWIFKGPASVALLVNLVFLIRIMWVLITKLRSAHTLETRQYYKASKALLVLIPLFGITYLLVLTGPEQGISRNLFEAIRAFLISTQGFFVALFYCFLNSEVRQTLRHRFIRWRESRNIHRSSSIKNRSTEECVICLRPTPHTRLATQEQNHSLNITDFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G780</accession>
    <name>A0AAU9G780_DROMD</name>
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        <recommendedName>
            <fullName evidence="5">UDENN domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="6" type="ORF">DMAD_03362</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
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        <property type="project" value="UniProtKB-KW"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG04375.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="942" mass="103400" checksum="68F30611561B404C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ERQ8</accession>
    <name>A0AAU9ERQ8_DROMD</name>
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        <submittedName>
            <fullName evidence="4">Flocculation protein FLO11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_08302</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052976">
        <property type="entry name" value="Scoloptoxin-like"/>
    </dbReference>
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        <property type="entry name" value="FI18007P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22933:SF42">
        <property type="entry name" value="FI18455P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043919528" evidence="2">
        <location>
            <begin position="38"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="3">
        <location>
            <begin position="110"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="939"/>
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        </location>
    </feature>
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        <location>
            <begin position="268"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="358"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="377"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="629"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="710"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="745"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="877"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="346"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="648"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="684"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="752"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="877"/>
            <end position="891"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF89230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="949" mass="106418" checksum="689B280B72C1CB10" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ERU3</accession>
    <name>A0AAU9ERU3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_08386</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9ERU3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034329">
        <property type="term" value="P:cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06647">
        <property type="entry name" value="STKc_PAK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000011">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000069">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="18">
        <location>
            <begin position="83"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="445"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="159"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="219"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="238"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFF89683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="719" mass="78068" checksum="B8721B5C872C43E6" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ERU6</accession>
    <name>A0AAU9ERU6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcription elongation regulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_08385</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9ERU6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000114">
        <property type="entry name" value="Transcription elongation regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <strain evidence="13 14">RF1</strain>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ES88</accession>
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        <submittedName>
            <fullName evidence="13">Protein mini spindles</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07932</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TOG/XMAP215 family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF89102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061863">
        <property type="term" value="F:microtubule plus end polymerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000052">
        <property type="entry name" value="Cytoskeleton associated protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000019">
        <property type="entry name" value="Cytoskeleton-associated protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000050">
        <property type="entry name" value="Cytoskeleton-associated protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000410">
        <property type="entry name" value="Mini spindles, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000063">
        <property type="entry name" value="Putative cytoskeleton-associated protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045110">
        <property type="entry name" value="XMAP215"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12609">
        <property type="entry name" value="MICROTUBULE ASSOCIATED PROTEIN XMAP215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="12">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="12">
        <location>
            <begin position="268"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="10">
        <location>
            <begin position="440"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="12">
        <location>
            <begin position="576"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="12">
        <location>
            <begin position="848"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="12">
        <location>
            <begin position="1181"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="225"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="507"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="799"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1102"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1410"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1453"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1594"/>
            <end position="1616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="225"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="251"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="550"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="799"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="828"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1105"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1466"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1594"/>
            <end position="1604"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025722"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF89102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2099" mass="231083" checksum="47AEE20C771C5A74" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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        <name evidence="6" type="ORF">DMAD_09445</name>
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        <name type="scientific">Drosophila madeirensis</name>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <strain evidence="6 7">RF1</strain>
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    </dbReference>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="EMBL" id="BFF91084.1"/>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F3R1</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        </source>
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                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F3X0</accession>
    <name>A0AAU9F3X0_DROMD</name>
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        </lineage>
    </organism>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="18 19">RF1</strain>
        </source>
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            <text>Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.</text>
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        </reaction>
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            <name>Zn(2+)</name>
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        </subcellularLocation>
    </comment>
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    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="12 15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8 15" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15 17" id="KW-0862">Zinc</keyword>
    <feature type="active site" evidence="16">
        <location>
            <position position="444"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
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        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="501"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001336"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012063"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00014713"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR007828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR007828-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR007828-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFF91279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="722" mass="82004" checksum="B6BC1407F7572430" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F417</accession>
    <name>A0AAU9F417_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="7">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_10115</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F417"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000092">
        <property type="entry name" value="Carboxylic ester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043106158" description="Carboxylic ester hydrolase" evidence="7">
        <location>
            <begin position="31"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="8">
        <location>
            <begin position="100"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF91956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="653" mass="73266" checksum="148B6BE43A172668" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F421</accession>
    <name>A0AAU9F421_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Gamma-aminobutyric acid type B receptor subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">G-protein coupled receptor 51</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_09701</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F421"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038039">
        <property type="term" value="C:G protein-coupled receptor heterodimeric complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004965">
        <property type="term" value="F:G protein-coupled GABA receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15294">
        <property type="entry name" value="7tmC_GABA-B-R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06366">
        <property type="entry name" value="PBP1_GABAb_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000063">
        <property type="entry name" value="Gamma-aminobutyric acid type B receptor subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000072">
        <property type="entry name" value="Gamma-aminobutyric acid type B receptor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002455">
        <property type="entry name" value="GPCR3_GABA-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519">
        <property type="entry name" value="GABA-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519:SF74">
        <property type="entry name" value="GAMMA-AMINOBUTYRIC ACID TYPE B RECEPTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01177">
        <property type="entry name" value="GABAB1RECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01176">
        <property type="entry name" value="GABABRECEPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12 23" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 21" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043840768" description="Gamma-aminobutyric acid type B receptor subunit 2" evidence="21">
        <location>
            <begin position="23"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="487"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="562"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="620"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="661"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="684"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="22">
        <location>
            <begin position="446"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="916"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1052"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="934"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="960"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="987"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1081"/>
            <end position="1091"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BFF91414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1223" mass="138550" checksum="27857F43EE458944" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F428</accession>
    <name>A0AAU9F428_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc finger protein rotund</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_08290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F428"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="433"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF89555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="514" mass="53494" checksum="982975E30A7EEE0C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F473</accession>
    <name>A0AAU9F473_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Nuclear pore membrane glycoprotein 210-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_07731</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUP210 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058779">
        <property type="entry name" value="Ig_NUP210_13th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057586">
        <property type="entry name" value="Ig_NUP210_16th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055096">
        <property type="entry name" value="Ig_NUP210_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055097">
        <property type="entry name" value="Ig_NUP210_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055098">
        <property type="entry name" value="Ig_NUP210_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056897">
        <property type="entry name" value="Ig_NUP210_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056898">
        <property type="entry name" value="Ig_NUP210_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055099">
        <property type="entry name" value="Ig_NUP210_7th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056899">
        <property type="entry name" value="Ig_NUP210_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045197">
        <property type="entry name" value="NUP210-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23019:SF0">
        <property type="entry name" value="NUCLEAR PORE MEMBRANE GLYCOPROTEIN 210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23019">
        <property type="entry name" value="NUCLEAR PORE MEMBRANE GLYCOPROTEIN GP210-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26181">
        <property type="entry name" value="Ig_NUP210_13th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25354">
        <property type="entry name" value="Ig_NUP210_16th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22967">
        <property type="entry name" value="Ig_NUP210_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22969">
        <property type="entry name" value="Ig_NUP210_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22963">
        <property type="entry name" value="Ig_NUP210_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24991">
        <property type="entry name" value="Ig_NUP210_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24935">
        <property type="entry name" value="Ig_NUP210_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22962">
        <property type="entry name" value="Ig_NUP210_7th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24902">
        <property type="entry name" value="Ig_NUP210_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="Invasin/intimin cell-adhesion fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043314107" evidence="10">
        <location>
            <begin position="22"/>
            <end position="1855"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1776"/>
            <end position="1793"/>
        </location>
    </feature>
    <feature type="domain" description="NUP210 Ig-like" evidence="13">
        <location>
            <begin position="22"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="NUP210 Ig-like" evidence="14">
        <location>
            <begin position="120"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="NUP210 Ig-like" evidence="12">
        <location>
            <begin position="234"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="NUP210 fourth Ig-like" evidence="17">
        <location>
            <begin position="336"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="NUP210 Ig-like" evidence="16">
        <location>
            <begin position="528"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="NUP210 Ig-like" evidence="11">
        <location>
            <begin position="633"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="NUP210 Ig-like" evidence="15">
        <location>
            <begin position="892"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="domain" description="NUP210 Ig-like" evidence="19">
        <location>
            <begin position="1160"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="domain" description="NUP210 Ig-like" evidence="18">
        <location>
            <begin position="1455"/>
            <end position="1499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF22969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF24902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF24935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF24991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF25354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF26181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF88831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1855" mass="208025" checksum="BF1365F3BA7AB0AA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F474</accession>
    <name>A0AAU9F474_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Guanylate cyclase</fullName>
            <ecNumber evidence="3 12">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_08389</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="3 12"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001653">
        <property type="term" value="F:peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000801">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000039">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF462">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
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    <keyword evidence="9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
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        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
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            <begin position="28"/>
            <end position="1386"/>
        </location>
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            <begin position="722"/>
            <end position="746"/>
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            <begin position="837"/>
            <end position="1123"/>
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            <begin position="1195"/>
            <end position="1324"/>
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            <end position="850"/>
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            <end position="1159"/>
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        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
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            <begin position="157"/>
            <end position="173"/>
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            <end position="199"/>
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        </source>
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        </source>
    </evidence>
    <sequence length="1386" mass="154068" checksum="9E7537CA6F413D6B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F479</accession>
    <name>A0AAU9F479_DROMD</name>
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        <recommendedName>
            <fullName evidence="10">Elongation of very long chain fatty acids protein</fullName>
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            <fullName evidence="10">Very-long-chain 3-oxoacyl-CoA synthase</fullName>
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        <name evidence="12" type="ORF">DMAD_07743</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12 13">RF1</strain>
        </source>
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            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <sequence length="377" mass="43002" checksum="B6352A93A68090AA" modified="2024-11-27" version="1">MALIMKYIDTVTRYLDSHGDSRTKDWPMMSSPFPTLAVCLTYVYLVKVLGPRLMENRKPLHLQNTLVLYNAIQVVFSAWLFYECLMGGWWGAYSFRCQPVDYTDSATSRRMVHACWWYYFSKFTEFMDTIFFVLRKKTSQVTTLHVIHHGCMPMSVWFGVKFTPGGHSTFFGLLNTFVHIVMYTYYMFSAMGPQYQKYLWWKKYLTTLQMVQFILIMVHAFQLLFIDCNYPKAFVWWIGMHAVMFFFLFNEFYKAAYRNRLMKKNALLANGHAKPNGYCKSINAHDDLAMPQPTAATVTPVSKANGSVAPLTNGHANGLSNGYGKQLANGNAPAMTTTQNGSGHKGGAGPNGLLTNGYATKLLDDASQDLKQRKTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F486</accession>
    <name>A0AAU9F486_DROMD</name>
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        <submittedName>
            <fullName evidence="4">Synapse-associated protein of 47 kDa</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ORF">DMAD_08415</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019:SF6">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD" evidence="3">
        <location>
            <begin position="299"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="202"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="485"/>
            <end position="546"/>
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        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
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        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
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        <location>
            <begin position="186"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="519"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="531"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF89720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="546" mass="56597" checksum="C4503CD333D2DF4C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F493</accession>
    <name>A0AAU9F493_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Histone acetyltransferase type B catalytic subunit</fullName>
            <ecNumber evidence="3 11">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_09857</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the HAT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3 11"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F493"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.360.10">
        <property type="entry name" value="Histone acetyl transferase 1 (HAT1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048776">
        <property type="entry name" value="HAT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019467">
        <property type="entry name" value="Hat1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037113">
        <property type="entry name" value="Hat1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017380">
        <property type="entry name" value="Hist_AcTrfase_B-typ_cat-su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013523">
        <property type="entry name" value="Hist_AcTrfase_HAT1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12046">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE TYPE B CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21183">
        <property type="entry name" value="HAT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10394">
        <property type="entry name" value="Hat1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038084">
        <property type="entry name" value="HAT-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histone acetyl transferase HAT1 N-terminal" evidence="17">
        <location>
            <begin position="11"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="16">
        <location>
            <begin position="179"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Histone acetyltransferase type B catalytic subunit C-terminal" evidence="18">
        <location>
            <begin position="273"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="13">
        <location>
            <begin position="47"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="13">
        <location>
            <begin position="212"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="367"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="12">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="235"/>
            <end position="241"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with histone H4 N-terminus" evidence="14">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR038084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF10394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF21183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFF91624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="404" mass="47776" checksum="DD76B9B7C840ED03" modified="2024-11-27" version="1">MAQIQEYQEYLLDALEVVDFKLIRDKADINNDAMIFHPAMAHQIFGETETIFGYQDLHVRVLYTAGPLHIYLGVEYGSRVNEVSGGEMNADDVVATIAQSLPDGCYFINLEEFLKTLDKADKFQPFGEKIGEYTRPSDDGSERLFEIYQCDYKVQSFLKFFARLQTFILWFVDAASYIDTDDAQWCYFVCYEKYKNGDGQYQYATAGYTTVYEYYAYPQNKRPRISQMMILPPFQKLGLATQLVETIYKYYQSQKNVVDITVEDPSEDFQRLRNFVDARFCKELKSFSRAEIVNGFSKEMVREARETLKLNPRQVRKVYELLRLYYTNVSDDKEYRNYRLEVKKRLNAVYYKQLKDLKKMERHKMDTEMMRAQLPNMKQRMEQLQEEYAVVEKDYKSTIAKLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDR2</accession>
    <name>A0AAU9FDR2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Venom dipeptidyl peptidase 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_11526</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Endomembrane system</location>
            <topology evidence="12">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9B family. DPPIV subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDR2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF200">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 10, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="17">
        <location>
            <begin position="123"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="16">
        <location>
            <begin position="711"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="527"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="600"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="542"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFF93734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="923" mass="103964" checksum="A717EFAD7630CDD3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDR4</accession>
    <name>A0AAU9FDR4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="13">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_11599</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="13"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDR4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044322">
        <property type="term" value="C:endoplasmic reticulum quality control compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904380">
        <property type="term" value="P:endoplasmic reticulum mannose trimming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000008">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044674">
        <property type="entry name" value="EDEM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679:SF2">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0834">Unfolded protein response</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043493726" description="alpha-1,2-Mannosidase" evidence="14">
        <location>
            <begin position="38"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="15">
        <location>
            <begin position="657"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="406"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="492"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF93826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="801" mass="90236" checksum="AD61C6B8340E7A99" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDR8</accession>
    <name>A0AAU9FDR8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ethanolamine-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CTP:phosphoethanolamine cytidylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_11596</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.14" evidence="10"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDR8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004306">
        <property type="term" value="F:ethanolamine-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02173">
        <property type="entry name" value="ECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044608">
        <property type="entry name" value="Ect1/PCYT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780:SF2">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="13">
        <location>
            <begin position="40"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Cytidyltransferase-like" evidence="13">
        <location>
            <begin position="224"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF93819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="388" mass="43372" checksum="D20BE6C20869832D" modified="2024-11-27" version="1">MSDKQVNGCSSAKSYSSPCNGNTTQKQEESPINGKDVRVWCDGCYDMVHFGHANSLRQAKALGDKVIVGIHTDAEITKHKGPPVFTEEERVKMVKGIKWVDEVVLGAPYVTTLEVLDQNNCDFCVHGDDITMTAEGVDTYHLVKSANRYKEVRRTAGVSTTDLVGRMLLLTRNHFRQGSAEYDIEKEAAKSPWTGCSQFLPTTQKIIQFSDGKSPNAGDKIVYVAGAFDLFHVGHLDFLEKASKLGDYLIVGLHTDPVVNSYKGSNYPIMNLHERVLSVLACKFVNEVVIGAPYCVTEELLEHFKIDVVCHGRTPIAMEDGKIDPYAVPKTKAIFELIDSGNEMTTERIVERIISHRLEYERRNQAKEKKEIEAFEALQRQKQTQKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDS2</accession>
    <name>A0AAU9FDS2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fibrinogen C domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_11573</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDS2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF327">
        <property type="entry name" value="FI21813P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF93792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="79" mass="9243" checksum="66A97EB19DE12C50" modified="2024-11-27" version="1">MKFSTLDRDNDLSEKRNCADNGGGWWFLNCFLSSLNGYYYADGKRIATQPYGIHWGSWKDYDYTISLTFAQMMIRPKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDS4</accession>
    <name>A0AAU9FDS4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Sialin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_11537</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDS4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000423">
        <property type="entry name" value="Putative inorganic phosphate cotransporter-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000003">
        <property type="entry name" value="Solute carrier family 17 member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF415">
        <property type="entry name" value="AT30085P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="26"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="447"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="449"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF93749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="479" mass="52387" checksum="6A5D0575F1A7EFCA" modified="2024-11-27" version="1">MDHRDDSRPEWGKSVSKVCLLPQRVVLVLMGFFSLICAFTCRVSLSHAITILAISNKISNKTIANLTDEAPDGVCQQEVESKDAVEHIGIYTWSETEKGFILGSFYFGYLISHLPGGVLADKYGAKWVVAFCVGVTAICELLSPTSIRLGPYYLITLRVLMGIAQGPIFPGLNGLLSSWAPKKERATLGTICFGGSTMGIILSNACSGLLLHYYTWPVMFYVFGSLSVIWFFVFAIICTSSPFSHPFITPKEREYLADEVGNVNKDKPAIPWKPLLLSMPMLSLIVSQMGHDWGYYVMVTNLPLYMADVIRFSIRSNGFVTALPYVAMWIASLISGVVADWLIRSGKLRLVTVRKLFTLLSAVLPGTFMVAASYAGCDKALVVALFTISMWTMGMYYAGQKLSALDISPSYSSTITAVINGLGSFTGLFAPPIIGLLTPDAEDRLFRGSHTNGESESKSIFTQPSGHSPAFQGVSEEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDS5</accession>
    <name>A0AAU9FDS5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">E3 ubiquitin-protein ligase lubel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_11613</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RBR family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071797">
        <property type="term" value="C:LUBAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990450">
        <property type="term" value="F:linear polyubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097039">
        <property type="term" value="P:protein linear polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19815">
        <property type="entry name" value="Bbox1_HOIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20337">
        <property type="entry name" value="BRcat_RBR_HOIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16631">
        <property type="entry name" value="mRING-HC-C4C4_RBR_HOIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20351">
        <property type="entry name" value="Rcat_RBR_HOIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047543">
        <property type="entry name" value="Bbox1_RNF31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047540">
        <property type="entry name" value="BRcat_RBR_RNF31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047542">
        <property type="entry name" value="Rcat_RBR_RNF31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026254">
        <property type="entry name" value="RNF31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032065">
        <property type="entry name" value="RNF31-UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041031">
        <property type="entry name" value="RNF31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047541">
        <property type="entry name" value="RNF31_RBR_mRING-HC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16004:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE LUBEL"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDV6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097003">
        <property type="term" value="F:adipokinetic hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15382">
        <property type="entry name" value="7tmA_AKHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000275">
        <property type="entry name" value="Gonadotropin-releasing hormone II receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF59">
        <property type="entry name" value="ADIPOKINETIC HORMONE RECEPTOR, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="59"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF93831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="452" mass="51075" checksum="26FBEFDF204D1B55" modified="2024-11-27" version="1">MAHSGEINEVVYDHRILKDWSNVNNTNGTMHLSKDMIFNDGHRLSITVYSILFVISTIGNSTVLYLLTKRRLRGPLRIDIMLMHLAIADLMVTLLLMPLEIAWAWTVQWRSTDLMCRLMSFFRVFGLYLSSFVMVCISLDRYYAILKPLQRSYNRGRIMLACAWMGSVICSIPQAFLFHLEEHPVVRGYFQCVTFHSFVSDFDNWLYQIATMCAMYAFPLIVFIYCYGAIYLEIYRKSQRVLKDVIAERFRRSNDDVLSRAKKRTLKMTISIVIVFIICWTPYYTICMWYSLDKTSVDKVNSLVRKALFIFASTNSCMNPLVYGLYNIRGRMNNNNNVSVNNRHTSLSNRLDSSNQLMQKPAAAANNSSLANRHGTVMAAAVAATTKLAHVVGLKGNGSNGSATASTIASAPPLPLPVTAPLASDDVDGDNDDSSVSVVTIRCQEPPRICHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDV7</accession>
    <name>A0AAU9FDV7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Sodium/potassium/calcium exchanger Nckx30C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_11634</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDV7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000002">
        <property type="entry name" value="Sodium/potassium/calcium exchanger 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000004">
        <property type="entry name" value="Sodium/potassium/calcium exchanger 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00367">
        <property type="entry name" value="calcium/sodium antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF72">
        <property type="entry name" value="SODIUM_POTASSIUM_CALCIUM EXCHANGER NCKX30C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0769">Symport</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="410"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="445"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="469"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="660"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="696"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="738"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="762"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="792"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="16">
        <location>
            <begin position="346"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="16">
        <location>
            <begin position="662"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="222"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="284"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="508"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="598"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="508"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF93878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="820" mass="89491" checksum="7C48E9F1FBF93EC7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDV8</accession>
    <name>A0AAU9FDV8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Sodium/potassium/calcium exchanger Nckx30C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_11634</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDV8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000002">
        <property type="entry name" value="Sodium/potassium/calcium exchanger 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000004">
        <property type="entry name" value="Sodium/potassium/calcium exchanger 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00367">
        <property type="entry name" value="calcium/sodium antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF72">
        <property type="entry name" value="SODIUM_POTASSIUM_CALCIUM EXCHANGER NCKX30C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0769">Symport</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="410"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="445"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="469"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="729"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="765"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="807"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="831"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="861"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="16">
        <location>
            <begin position="346"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="16">
        <location>
            <begin position="731"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="222"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="284"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="517"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="521"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="551"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF93871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="889" mass="97075" checksum="4A733D05FA7FAAEA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDV9</accession>
    <name>A0AAU9FDV9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Sodium/potassium-transporting ATPase subunit beta-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_11616</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the X(+)/potassium ATPases subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDV9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005890">
        <property type="term" value="C:sodium:potassium-exchanging ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1660:FF:000004">
        <property type="entry name" value="sodium/potassium-transporting ATPase subunit beta-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1660">
        <property type="entry name" value="Na, k-atpase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000402">
        <property type="entry name" value="Na/K_ATPase_sub_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038702">
        <property type="entry name" value="Na/K_ATPase_sub_beta_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523">
        <property type="entry name" value="SODIUM/POTASSIUM-DEPENDENT ATPASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523:SF46">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00287">
        <property type="entry name" value="Na_K-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0915">Sodium</keyword>
    <keyword evidence="16" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0740">Sodium/potassium transport</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF93853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="322" mass="37062" checksum="BFBEEA3136D7B2E8" modified="2024-11-27" version="1">MSKPQPMSKSFTDEDMGNIRKPRPFHLGQFLYNSETGTVMNRDRASWAKIGIFYVAFYGVLGALVAICMWAFFQTLDPRIPKWTLDESIIGTNPGLGFRPLPPMDNVESTLIWYKGTQHENYKHWTDSLDDFLAVYKVPGLTPGRGQNIYNCDYNQPPPKGQVCDVDIKSWTPCTKENNYSYHKSAPCIFLKLNKIYGWIPEYYNDSRDLPESMPLSLKTYIGEVEKTQPHKLNTIWVSCEGENPADQENIGAVNYLPVRGFPGYFYPYQNSEGYLSPLVAVHFQRPRRGIIINVECKAWARNIIHDRKERIGSVHYELLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDW1</accession>
    <name>A0AAU9FDW1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc carboxypeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_11642</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDW1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03860">
        <property type="entry name" value="M14_CP_A-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000001">
        <property type="entry name" value="Carboxypeptidase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF156">
        <property type="entry name" value="RH39904P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 14" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043627938" evidence="12">
        <location>
            <begin position="20"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="13">
        <location>
            <begin position="119"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF93888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="424" mass="48194" checksum="88AD424E030584A5" modified="2024-11-27" version="1">MKVLPVSLSLVSLVALCLGERLTFTDHRVYRLEPKTEAQLEGLRPLEATNERVIFLNELHLGAFNVLISPSYNEEFLQLLQRLKLAPELLDANAQESLSLDIEPVSDANRRSDDYSWTEYHELNDTHRWMQQLVEKNQGIVTTFVAGRSYEGRELLGLRISHSAGVERQAIFLEAGMHAREWISPATATYFANQLLSSQQPEIQSLARSYVWYILPHANPDGYVYTHSTNRLWRKTRSPQEKNCMGSDPNRNWGFHWREVGASDEPCSESYAGPKAFSEPETESLSQYLMSLPEPVFMYVSLHSFSQLLLYPYGHTSALPENHRQLERIFSAALGGMKRRFGTRYTGGNVYDAIYPAAGSSMDWAYGVLKTKYSFTYELRPSGYSFWTGFRLPASQIIPTGQETTDSLVEMIKAAASIEGYELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDW2</accession>
    <name>A0AAU9FDW2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Sodium channel protein Nach</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_11641</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDW2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF253">
        <property type="entry name" value="PICKPOCKET 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12 14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF93885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="416" mass="48032" checksum="316786E454B4686A" modified="2024-11-27" version="1">MTERLLVLAQFYLNPLETPRLDVAQLQRLHWLLTSYHSDTGGYSVRDILRQLSPDCGDLIIRGIVYGSKVNTSQMFVKRPTSSNVCCIFNYRRPSYSRFARIREEDNAEMRAQPKVVFESNSILNSVQFVLQDTPENDRTNTFMTNNAFQEDYATVQSTTLGEMLVDHNTIVEIPIQPTFFSSSEGVRTVPPQARRCYFPEEGRKVLNQTYYSIDECLLLCRSQSMMNHCGCVSPPMIGSSTNVTYCGLPDLPCLSKWKSIWYGYDEYPYLQEGKDQMAQRECTHCLPTCNGVSFSITSNVAPLRRNYNETGYLMGLLKGLTNERPLAVIKFFFKLRFAQATKSELVSTWVVLLNRFGGILSLMYGFSIVSYIEIFYYLTGKWFVYIYRSRSKRTVRAGHSLYWDELMPKRFRTPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDW6</accession>
    <name>A0AAU9FDW6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Sodium/potassium/calcium exchanger Nckx30C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_11634</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDW6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000002">
        <property type="entry name" value="Sodium/potassium/calcium exchanger 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NaCa_Exmemb"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
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    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0769">Symport</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
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            <begin position="410"/>
            <end position="433"/>
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
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            <end position="740"/>
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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            <begin position="818"/>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="848"/>
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            <begin position="346"/>
            <end position="487"/>
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        <location>
            <begin position="718"/>
            <end position="865"/>
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            <begin position="1"/>
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            <begin position="58"/>
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            <begin position="155"/>
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    <feature type="region of interest" description="Disordered" evidence="14">
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            <begin position="222"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
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            <begin position="284"/>
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        <location>
            <begin position="10"/>
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            <begin position="62"/>
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    </feature>
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    </feature>
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        <location>
            <begin position="231"/>
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    </feature>
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        <location>
            <begin position="305"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
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            <begin position="508"/>
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    </feature>
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        <location>
            <begin position="538"/>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF93872.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="876" mass="95619" checksum="E4E971A6B6B48D17" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDX2</accession>
    <name>A0AAU9FDX2_DROMD</name>
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        <recommendedName>
            <fullName evidence="6">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_11667</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
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            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF93919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome U"/>
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    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07830">
        <property type="entry name" value="STKc_MAK_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="serine/threonine-protein kinase MAK isoform X1"/>
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    <dbReference type="FunFam" id="3.30.200.20:FF:000071">
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="MAPK"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13 24" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="4"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="373"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="587"/>
            <end position="611"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="634"/>
            <end position="669"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="394"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="645"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="692" mass="76969" checksum="6515022A5E35C862" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDX5</accession>
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        <submittedName>
            <fullName evidence="12">E3 ubiquitin-protein ligase lubel</fullName>
        </submittedName>
    </protein>
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        <name evidence="12" type="ORF">DMAD_11613</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the RBR family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0097039">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BRcat_RBR_HOIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16631">
        <property type="entry name" value="mRING-HC-C4C4_RBR_HOIP"/>
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    </dbReference>
    <dbReference type="CDD" id="cd20351">
        <property type="entry name" value="Rcat_RBR_HOIP"/>
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    </dbReference>
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        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="Bbox1_RNF31-like"/>
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        <property type="entry name" value="RNF31-UBA"/>
    </dbReference>
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        <property type="entry name" value="RNF31_C"/>
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        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
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        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16004:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE LUBEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16004">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="UBA_HOIP"/>
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    </dbReference>
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        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="2495"/>
            <end position="2733"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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        </location>
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    </feature>
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    <feature type="region of interest" description="Disordered" evidence="9">
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    <feature type="region of interest" description="Disordered" evidence="9">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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    <feature type="region of interest" description="Disordered" evidence="9">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
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        </location>
    </feature>
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        </location>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
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    </feature>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1906"/>
            <end position="1915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2877" mass="320057" checksum="21EAC24B1CC68A1B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FE32</accession>
    <name>A0AAU9FE32_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase</fullName>
        </alternativeName>
    </protein>
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        <name evidence="8" type="ORF">DMAD_11671</name>
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    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nudix hydrolase family.</text>
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        <property type="evidence" value="ECO:0007669"/>
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    <dbReference type="GO" id="GO:0000166">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006167">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006754">
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        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NUDIX_hydrolase_CS"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR051325">
        <property type="entry name" value="Nudix_hydrolase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003565">
        <property type="entry name" value="Tetra_PHTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21340:SF0">
        <property type="entry name" value="BIS(5'-NUCLEOSYL)-TETRAPHOSPHATASE [ASYMMETRICAL]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21340">
        <property type="entry name" value="DIADENOSINE 5,5-P1,P4-TETRAPHOSPHATE PYROPHOSPHOHYDROLASE MUTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01405">
        <property type="entry name" value="TETRPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF93924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="120" mass="13866" checksum="FBCFBEA2D0D01C0C" modified="2024-11-27" version="1">MKKAAGFHWSPPKGHVDPGEDDFTTALRETKEEAGYDENDLIIDKNTPITLNYQVKKEPKIVIYWLAELRNPNQEPILSEEHTELKWLGKEAAKDCAGFKDFQVMIDKFHEVIVTREKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FE36</accession>
    <name>A0AAU9FE36_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sorting nexin-17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_11657</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FE36"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13337">
        <property type="entry name" value="FERM-like_C_SNX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000599">
        <property type="entry name" value="AGAP011491-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.60:FF:000001">
        <property type="entry name" value="Sorting nexin-17 isoform1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048763">
        <property type="entry name" value="SNX17-31_FERM_F1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048767">
        <property type="entry name" value="SNX17-31_FERM_F2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040842">
        <property type="entry name" value="SNX17/31_FERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037836">
        <property type="entry name" value="SNX17_FERM-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431:SF14">
        <property type="entry name" value="LD15323P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431">
        <property type="entry name" value="SORTING NEXIN 17 AND 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21273">
        <property type="entry name" value="SNX17-27-31_F1_FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21271">
        <property type="entry name" value="SNX17-31_F2_FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18116">
        <property type="entry name" value="SNX17_FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="5">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="342"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="358"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF93906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="444" mass="49674" checksum="698BF1BF482BD0C6" modified="2024-11-27" version="1">MRRHCSSLSLPPFPPKRFLPLTNGQLETRRSGLEQYLQIIGQDARIARLPFLQQFFQRAQMDTALAEGNVASDSEEKQLEVEVYTNPNGAQLLINCNVQQNASLLLKSVCRELKLPDELMKLFCLYLMRKKRESDELVLVRRLMDFESPYITRLHAQPCELLLRTCYWDSSWDAKLYGNKVALNLLYNQTVADVSREWVVICSPGLGRQLSHLQMQGRCLEYMNVVRDLPSYGCLQFEAANVDYPDPKTVALVSIGNKELSLRTARGAKIYETKFRVTRMRSWRVSVTHNALESRHEPSQLQLAFEYLIAKQTLRWITISSDQAMLMSVCLQAMVDQLLHGGGGKPAPSSDDEQDAGATSSASTPSSVSSTSTSTCASTSYSTTTTASMTTPPVKFLAQDLTPKSKQQRLTTSRTFGAVFFRNDGEAGAHVANEAFEGIGDDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FE39</accession>
    <name>A0AAU9FE39_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mitoguardin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_11773</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FE39"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF94046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="90" mass="10171" checksum="3903F381CFD48CA3" modified="2024-11-27" version="1">MQLGILGMGALDTVIDFWEDASAVHYSAQHSEFDDEVQKLLQMAYTLQEQSRLIFCDETQDGYLSKSASESLSLRSSCDSTYSHTSEMYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FE47</accession>
    <name>A0AAU9FE47_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_11667</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FE47"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07830">
        <property type="entry name" value="STKc_MAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000104">
        <property type="entry name" value="serine/threonine-protein kinase MAK isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000071">
        <property type="entry name" value="serine/threonine-protein kinase MAK isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13 24" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="4"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="373"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="587"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="634"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="394"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="645"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="667"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="BFF93920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="696" mass="77331" checksum="A457FE36AA10EE03" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FE72</accession>
    <name>A0AAU9FE72_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Leucine-rich repeats and immunoglobulin-like domains protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_11722</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FE72"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050328">
        <property type="entry name" value="Dev_Immune_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373:SF378">
        <property type="entry name" value="FI03225P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373">
        <property type="entry name" value="SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043347533" evidence="5">
        <location>
            <begin position="26"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="600"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="607"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="623"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF93985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="649" mass="74857" checksum="BEBFB44629A134E0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FE81</accession>
    <name>A0AAU9FE81_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Aromatic-L-amino-acid decarboxylase</fullName>
            <ecNumber evidence="8">4.1.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DOPA decarboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_11755</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.28" evidence="8"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FE81"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004058">
        <property type="term" value="F:aromatic-L-amino-acid decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042423">
        <property type="term" value="P:catecholamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042427">
        <property type="term" value="P:serotonin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06450">
        <property type="entry name" value="DOPA_deC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1340.10:FF:000001">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000025">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1340.10">
        <property type="entry name" value="dopa decarboxylase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF167">
        <property type="entry name" value="AROMATIC-L-AMINO-ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00800">
        <property type="entry name" value="YHDCRBOXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0127">Catecholamine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 11 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF94024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="476" mass="53296" checksum="8D683B21B8E873AB" modified="2024-11-27" version="1">MEAPEFKDFAKSMVDYIAEYLENIRDRRVLPEVKPGYLKPLIPDAAPEKPESWQDVMQDIERVIMPGVTHWHSPKFHAYFPTANSYPAIVADMLSGAIACIGFTWIASPACTELEVAMMDWLGKMIDLPAEFLACSGGKGGGVIQGTASESTLVALLGAKAKKVKEVQAEHPDWDEHTIIGKLVGYCSDQAHSSVERAGLLGGVKLRSVASDNSRMRGEALEKAIKEDLENGLIPFYAVVTLGTTNSCAFDRLDECGPVGNKHNVWIHVDAAYAGSAFICPEYRHLMKGIETADSFNFNPHKWMLVNFDCSAMWLKDPSWVVNAFNVDPLYLKHDMQGSAPDYRHWQIPLGRRFRALKLWFVLRLYGVENLQAHIRRHCNFATQFGELAVADSRFELAAEVNMGLVCFRLKGSNERNEALLKRINGRGNIHMVPAKIKDVYFLRMAVCSRFTRSEDMVYTWNEVSAAATEEIEAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FE92</accession>
    <name>A0AAU9FE92_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Enoyl-CoA delta isomerase 1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">3,2-trans-enoyl-CoA isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_11664</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Key enzyme of fatty acid beta-oxidation. Able to isomerize both 3-cis (3Z) and 3-trans (3E) double bonds into the 2-trans (2E) form in a range of enoyl-CoA species, with a preference for (3Z)-enoyl-CoAs over (3E)-enoyl-CoAs. The catalytic efficiency of this enzyme is not affected by the fatty acyl chain length.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2E)-tetradecenoyl-CoA = (3Z)-tetradecenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:29847"/>
            <dbReference type="ChEBI" id="CHEBI:61405"/>
            <dbReference type="ChEBI" id="CHEBI:61968"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:29849"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(3Z)-decenoyl-CoA = (2E)-decenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:77195"/>
            <dbReference type="ChEBI" id="CHEBI:61406"/>
            <dbReference type="ChEBI" id="CHEBI:195601"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:77196"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(3Z)-dodecenoyl-CoA = (2E)-dodecenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:23716"/>
            <dbReference type="ChEBI" id="CHEBI:57330"/>
            <dbReference type="ChEBI" id="CHEBI:58543"/>
            <dbReference type="EC" id="5.3.3.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:23717"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(3Z)-octenoyl-CoA = (2E)-octenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:46044"/>
            <dbReference type="ChEBI" id="CHEBI:62242"/>
            <dbReference type="ChEBI" id="CHEBI:85640"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:46045"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FE92"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004165">
        <property type="term" value="F:delta(3)-delta(2)-enoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000034">
        <property type="entry name" value="Enoyl-CoA delta isomerase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF45">
        <property type="entry name" value="ENOYL-COA DELTA ISOMERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9 17" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00083575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF93914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="253" mass="28020" checksum="127A0A912F9F5D7C" modified="2024-11-27" version="1">MISSRLVSLGARTGPRRFMSTAKLLLDIKSSIKEIENNSSRGLIITSASPKVFCAGLDILEMYKPDQQRLRAVWTELQNVWCALYGISVPTAAAINGHAPAGGCLLATACEYRVMVPDCRIGLNETQLGIIAPKWLVSGFLNVLPKRVAERALTQSRLFTTDEALQEGLIDEIATSKEEAVEKCAAFIGTFARVNSVARALTKQQFRADNLRQFHKEREQDVEIFLSLVNRPEVQKGIGLYLEGLKKKKANKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLL5</accession>
    <name>A0AAU9FLL5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Venom dipeptidyl peptidase 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05163</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9B family. DPPIV subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLL5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF192">
        <property type="entry name" value="IP17501P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="6">
        <location>
            <begin position="129"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="5">
        <location>
            <begin position="590"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00072929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF96547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="802" mass="90093" checksum="A93050D1CF86157A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLL7</accession>
    <name>A0AAU9FLL7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Restin homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_13590</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10 11">RF1</strain>
        </source>
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        <subcellularLocation>
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        </subcellularLocation>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
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        <property type="entry name" value="CLIP1_ZNF"/>
    </dbReference>
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        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF93">
        <property type="entry name" value="RESTIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16641">
        <property type="entry name" value="CLIP1_ZNF"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CAP_GLY_2"/>
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    </dbReference>
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    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CAP-Gly" evidence="9">
        <location>
            <begin position="159"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="9">
        <location>
            <begin position="275"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1349"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1666"/>
            <end position="1697"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="359"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1602"/>
            <end position="1629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="105"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1355"/>
            <end position="1365"/>
        </location>
    </feature>
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        <location>
            <begin position="1378"/>
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        </location>
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        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF96380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1722" mass="192947" checksum="B9A300B57EEB0713" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLL8</accession>
    <name>A0AAU9FLL8_DROMD</name>
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            <fullName evidence="2">lysozyme</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="9" type="ORF">DMAD_05325</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
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        </reaction>
    </comment>
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    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="lyz_i"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008597">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11195:SF22">
        <property type="entry name" value="LYSOZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05497">
        <property type="entry name" value="Destabilase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51909">
        <property type="entry name" value="LYSOZYME_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
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    <feature type="disulfide bond" evidence="7">
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        </location>
    </feature>
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            <begin position="147"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="182"/>
            <end position="188"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF96754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="250" mass="27220" checksum="4072D97F7642FAB2" modified="2024-11-27" version="1">MAMGMDRTSSSSVAELGAGDVEGSKVAAHQEYAEVLSLARHRRKRNIRSWSTLDLLSVAAVVIALLLILGAIYMHLKQRPHLGRLHIARKEKGKELVDTETLPISLDVAAGVAAQSITTLRPLTVETSEDCLLCIAVVATDNQPVTCGGKPCGIYRISWLYWMDAQGTSDPDNPLTQGYEGCVPVARCAEGLVRSYVRRFGYDCDGDGLIGCRDHVMLHLNGPTGCRMGKPLPALAELRMNKCFAKKDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLM2</accession>
    <name>A0AAU9FLM2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptide-methionine (S)-S-oxide reductase</fullName>
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        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Peptide-methionine (S)-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05095</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLM2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1060.10:FF:000004">
        <property type="entry name" value="Peptide methionine sulfoxide reductase A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049006">
        <property type="entry name" value="MsrA_helical"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774:SF1">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE MSRA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20939">
        <property type="entry name" value="MsrA_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="5">
        <location>
            <begin position="41"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Selenoprotein methionine sulfoxide reductase A helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF96451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="246" mass="27682" checksum="6712416091F2B9C9" modified="2024-11-27" version="1">MSLTIKTAVSHPELKDLRTVRNEQKELSILPVHHVNIPKATATFGMGCFWGAESLYGATRGVLRTTVGYAGGSSELPTYRKLGDHTEVLEIDYDPTVISFKELLDLFWNNHEYGLTTPIKRQYASLILYHDDEQKKMAVTSKLEEQERRAPELITTEVAPKVNFYAAEAYHQKYRLQGHKDLATSLNLSPTLLQSSYVATKLNGYLAGVGGMEQFKSEVDTLGLTPTQRQYCSYHVEQNEGQGLYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMC2</accession>
    <name>A0AAU9FMC2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Collagen alpha-1(XV) chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_05327</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMC2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005594">
        <property type="term" value="C:collagen type IX trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00247">
        <property type="entry name" value="Endostatin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000137">
        <property type="entry name" value="Multiplexin collagen isoform Ap3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000048">
        <property type="entry name" value="Multiplexin collagen isoform Ap3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1620.70:FF:000001">
        <property type="entry name" value="Multiplexin collagen isoform Ap3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010515">
        <property type="entry name" value="Collagenase_NC10/endostatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045463">
        <property type="entry name" value="XV/XVIII_trimerization_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1116">
        <property type="entry name" value="MACROPHAGE RECEPTOR WITH COLLAGENOUS STRUCTURE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20010">
        <property type="entry name" value="Collagen_trimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06482">
        <property type="entry name" value="Endostatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0176">Collagen</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043325348" evidence="4">
        <location>
            <begin position="21"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="domain" description="Thrombospondin-like N-terminal" evidence="5">
        <location>
            <begin position="32"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="282"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="376"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="394"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="427"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="447"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="466"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="565"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="579"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="629"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF96762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1007" mass="106037" checksum="B1E1A884EA0416BC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMC4</accession>
    <name>A0AAU9FMC4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein I'm not dead yet</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_05272</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMC4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015137">
        <property type="term" value="F:citrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015141">
        <property type="term" value="F:succinate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031312">
        <property type="entry name" value="Na/sul_symport_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF82">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01271">
        <property type="entry name" value="NA_SULFATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="439"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="482"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="522"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF96689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="571" mass="63447" checksum="3A1E180D98A653DA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMC6</accession>
    <name>A0AAU9FMC6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">ATP-dependent DNA helicase Q5</fullName>
            <ecNumber evidence="20">5.6.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">DNA 3'-5' helicase RecQ5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">DNA helicase, RecQ-like type 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">RecQ protein-like 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">DMAD_05174</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29 30">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="20"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMC6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <location>
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        <location>
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        <source>
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        <source>
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    <sequence length="1014" mass="115650" checksum="115A88BF5E98624A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMG4</accession>
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15 16">RF1</strain>
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        <property type="entry name" value="PROTEIN SIDEKICK"/>
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</entry>
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</entry>
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        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Nuclear receptor subfamily 5 group A member 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_05267</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMH8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07167">
        <property type="entry name" value="NR_DBD_Lrh-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06944">
        <property type="entry name" value="NR_LBD_Ftz-F1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000032">
        <property type="entry name" value="Nuclear hormone receptor FTZ-F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000006">
        <property type="entry name" value="Nuclear receptor subfamily 5 group A member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016355">
        <property type="entry name" value="NR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086:SF15">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR FTZ-F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 5 GROUP A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="15">
        <location>
            <begin position="493"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="16">
        <location>
            <begin position="782"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="142"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="266"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="321"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="435"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="670"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="323"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="343"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="372"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="403"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="435"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="447"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="459"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="682"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="706"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF96681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1017" mass="106945" checksum="D4E339ECC41DEB14" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMI3</accession>
    <name>A0AAU9FMI3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Phosphatidate phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_05396</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMI3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03384">
        <property type="entry name" value="PAP2_wunen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000043">
        <property type="entry name" value="GD12067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF197">
        <property type="entry name" value="FI04477P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="62"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="280"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="298"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="314"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF96850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="334" mass="37657" checksum="887C773A5995D750" modified="2024-11-27" version="1">MRTFKRGFFCSDLTIRYPYHECTITVPMLLLMKLLLPMLLISVVEIMRICKRFRMRLYLRNLWRSQATFSFGFIATFLTTELAKHVVGRLRPHFYQACQPRLNDGTSCSDPQNADLYVEQFFCSNRNISARQIRELHVSFPSAHSSLSFYSMCLLAFYVHSVWQGRGSIRVLRHILQFLMLMAALCVSLSRVADYWHHWSDVLAGAVLGVVYAAITAIYVGDMLRPQRPRAAVPCPTALGYSGGCHHHHLHQLMAENNNSSTSTKVHFLAAAASATMTTTTPLDDIHNSNSNSNSNSDTDDNDDEDDDDDVYKPPTSRTSTTPSPSALDLPNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMJ0</accession>
    <name>A0AAU9FMJ0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NEDD4-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_05439</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMJ0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013899">
        <property type="entry name" value="DUF1771"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052772">
        <property type="entry name" value="Endo/PolyKinase_Domain-Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46535">
        <property type="entry name" value="NEDD4-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46535:SF1">
        <property type="entry name" value="NEDD4-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08590">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01162">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SMR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smr" evidence="2">
        <location>
            <begin position="841"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="734"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="734"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF96907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="930" mass="105063" checksum="E827992960A3C55B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FMJ1</accession>
    <name>A0AAU9FMJ1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="22">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_05354</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:18106"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Mn(2+)(in) + ATP + H2O = Mn(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66820"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:66821"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Monomer. Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Membrane</location>
            <topology evidence="22">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Sarcoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="22"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FMJ1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02085">
        <property type="entry name" value="P-type_ATPase_SPCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000008">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000006">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000017">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000106">
        <property type="entry name" value="E1-E2 ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006413">
        <property type="entry name" value="P-type_ATPase_IIA_PMR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01522">
        <property type="entry name" value="ATPase-IIA2_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 22" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 22" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17 22" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 22" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="405"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="881"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="951"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="981"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="23">
        <location>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="1024" mass="112466" checksum="F9C20D17B7D6B4A3" modified="2024-11-27" version="1"> </sequence>
</entry>
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        <name type="scientific">Drosophila madeirensis</name>
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            <authorList>
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                <person name="Yamamoto D."/>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUT5</accession>
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        <alternativeName>
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        <alternativeName>
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        </alternativeName>
    </protein>
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        <name evidence="20" type="ORF">DMAD_07260</name>
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    <organism evidence="20 21">
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        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    <sequence length="1883" mass="205622" checksum="8C787B6A16677AF0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
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            <taxon>Endopterygota</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Yamamoto D."/>
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            <end position="1138"/>
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            <begin position="1341"/>
            <end position="1363"/>
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        </source>
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        </source>
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        </source>
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            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
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            <taxon>Endopterygota</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="GABAARECEPTR"/>
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        <location>
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        <location>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUX3</accession>
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        <submittedName>
            <fullName evidence="9">Optomotor-blind protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="9" type="ORF">DMAD_00039</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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    </comment>
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        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="302" mass="33133" checksum="A65CF399E1D81502" modified="2024-11-27" version="1">MGEGLSQTQDWKDQIELDDDTADDETIMERRWQIGYEQYRILCEKYTEKKEETSPVSTRIAGGELADAGMFPYQVGLVLQLSNGDLIQCGGSLVTLQYVLTAAHCLTDAIGARIYLGALQLAERGAAAEEFLVSHKEFRIYPGYLGFGAYNDLALIRLPRPATASARVQTIALAGEFMQQKFLERKPVTVSGWGSLGDSAVERTRLLHYLDTEVLDQERCMCSFLPGLVSTRRHLCTDGLGGRGACGGDSGGPLVYQWQNVSHLIGITSFGSVGGCELGAPTVYTRITAYLPWIRQQTGMTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUX6</accession>
    <name>A0AAU9FUX6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">trypsin</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_07326</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
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            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="11"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="serine proteases 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000043">
        <property type="entry name" value="serine proteases 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12 15" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043650448" description="trypsin" evidence="13">
        <location>
            <begin position="17"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="14">
        <location>
            <begin position="40"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF99414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="274" mass="28343" checksum="350B6D87701395AC" modified="2024-11-27" version="1">MKVLVVFALALASASAGLLPQVLPVHPRDLPAVPSIEGRITNGQTASEGQFPYQVGLSFGSSSGSWWCGGSIIDNSWLLTAAHCTSGASDVTIYYGATVRTNAQLSQTVSNANFVQHASYNSVILRNDISLIKTPAVSFTSAINKVALPSIASSYSTYAGDTAVASGWGKTSDAASSVANTLQFSTFTVVTNANCAQTYGSLIINQGVLCIGTPNKVSTCNGDSGGPLVLKSSNVLIGVTSFVSSAGCESGDYAGFSRVTYFLDWIKSNSGVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUY0</accession>
    <name>A0AAU9FUY0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sin3 histone deacetylase corepressor complex component SDS3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_00034</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFF99904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUY0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013907">
        <property type="entry name" value="Sds3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21964">
        <property type="entry name" value="BREAST CANCER METASTASIS-SUPPRESSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08598">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01401">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="12"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF99904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="326" mass="38580" checksum="BBA9888E4A0E20C5" modified="2024-11-27" version="1">MSNYYSFYTRDNYDDESIGDERSEEDDASETEFRSPNHYGGTNGTSNSTNNKIGINSELKEQMYQHKLLNLQKQMVELNQLIHPEYIKRVKKLDNQLKERRRLNEIYKDYMRECVERDYILEKKAAQKEYDEKMMDLKDNLISDFEDRKRLIENERYSLELTNDSMEIKTTVTRKLRRRPNEPMPVIEKRRKPATGQLLVYQLDDKEIESDLKVIQRGKPLGPNILHQNGIVSYTNAQQQQSQMLLNDLTANSYVETRIEDNKLLYERRWFCRGQQVYVEGKDLSKFAATITAIGNEVVWVKRTNESKVKINMSHLAKGKISIKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUY1</accession>
    <name>A0AAU9FUY1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">5'-3' exoribonuclease 1</fullName>
            <ecNumber evidence="12">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_00022</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the 5'-3' exonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="12"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFF99887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000004">
        <property type="entry name" value="5'-3' exoribonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000005">
        <property type="entry name" value="5'-3' exoribonuclease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016494">
        <property type="entry name" value="5_3_exoribonuclease_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041385">
        <property type="entry name" value="SH3_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040992">
        <property type="entry name" value="XRN1_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047007">
        <property type="entry name" value="XRN1_D1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041106">
        <property type="entry name" value="XRN1_D2_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040587">
        <property type="entry name" value="XRN1_DBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047008">
        <property type="entry name" value="XRN1_SH3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF7">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18129">
        <property type="entry name" value="SH3_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18332">
        <property type="entry name" value="XRN1_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18334">
        <property type="entry name" value="XRN1_D2_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18245">
        <property type="entry name" value="XRN1_DBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006743">
        <property type="entry name" value="Exonuclease_Xnr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="15">
        <location>
            <begin position="269"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' exoribonuclease 1 D1" evidence="18">
        <location>
            <begin position="638"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease Xrn1 D2/D3" evidence="19">
        <location>
            <begin position="808"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' exoribonuclease 1 SH3-like" evidence="16">
        <location>
            <begin position="1060"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="domain" description="5-3 exonuclease XRN1 DCP1-binding motif" evidence="17">
        <location>
            <begin position="1341"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1152"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1245"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1458"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1178"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1245"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1459"/>
            <end position="1471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038299"/>
        </source>
    </evidence>
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        </source>
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        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF18129"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF18245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF18332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF18334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF99887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1546" mass="177977" checksum="88AFE894A01C99DF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUY3</accession>
    <name>A0AAU9FUY3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Protein nervous wreck</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07091</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:lipid binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:neuromuscular synaptic transmission"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:positive regulation of cytoskeleton organization"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:positive regulation of supramolecular fiber organization"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:regulation of actin filament polymerization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FCH_dom"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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        <location>
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        </location>
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        </location>
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        </location>
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        </location>
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        </location>
    </feature>
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        <location>
            <begin position="930"/>
            <end position="1005"/>
        </location>
    </feature>
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        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
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            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="497"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="713"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
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            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
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            <end position="846"/>
        </location>
    </feature>
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        <location>
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            <end position="885"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
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            <end position="977"/>
        </location>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF99110.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1023" mass="112428" checksum="E020F64FBDD5E040" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <source>
            <strain evidence="6 7">RF1</strain>
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    </dbReference>
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    </dbReference>
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        <location>
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        <location>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
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    </feature>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Endopterygota</taxon>
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            <taxon>Brachycera</taxon>
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            <taxon>Drosophilidae</taxon>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the spectrin family.</text>
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    </dbReference>
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    <dbReference type="GO" id="GO:0016328">
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0051693">
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    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:long-term strengthening of neuromuscular junction"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0048790">
        <property type="term" value="P:maintenance of presynaptic active zone structure"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3_Alpha_Spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SPEC"/>
        <property type="match status" value="13"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000263">
        <property type="entry name" value="Alpha spectrin, isoform C"/>
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    </dbReference>
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        <property type="entry name" value="Spectrin alpha chain non-erythrocytic 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000037">
        <property type="entry name" value="Spectrin alpha chain non-erythrocytic 1"/>
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    </dbReference>
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        <property type="entry name" value="spectrin alpha chain, non-erythrocytic 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000017">
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    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000020">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
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    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000035">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
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    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000043">
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    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
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        <property type="entry name" value="Spectrin_repeat"/>
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        <property type="match status" value="20"/>
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        <property type="entry name" value="SH3DOMAIN"/>
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        <property type="entry name" value="SPECTRNALPHA"/>
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        <property type="match status" value="2"/>
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    <dbReference type="SMART" id="SM01184">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00150">
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        <property type="match status" value="1"/>
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        <property type="match status" value="16"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0117">Actin capping</keyword>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
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    <keyword evidence="10" id="KW-0112">Calmodulin-binding</keyword>
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    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="970"/>
            <end position="1029"/>
        </location>
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    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="2265"/>
            <end position="2300"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="2308"/>
            <end position="2343"/>
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    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="293"/>
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    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="505"/>
            <end position="564"/>
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    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="822"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1216"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1641"/>
            <end position="1675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1892"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006826"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF99261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2415" mass="278371" checksum="5397FF84C2894427" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV41</accession>
    <name>A0AAU9FV41_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tight junction-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_07424</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043441">
        <property type="entry name" value="Tjap1/BEGAIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28664">
        <property type="entry name" value="TIGHT JUNCTION-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28664:SF4">
        <property type="entry name" value="TIGHT JUNCTION-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="135"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="686"/>
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        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="19"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="139"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="686"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="731"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF99547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="941" mass="101197" checksum="3797B6C2BEA04BA3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV43</accession>
    <name>A0AAU9FV43_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulator of MON1-CCZ1 complex</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_07266</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FV43"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040371">
        <property type="entry name" value="RMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009755">
        <property type="entry name" value="RMC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049040">
        <property type="entry name" value="RMC1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12897">
        <property type="entry name" value="COLON CANCER-ASSOCIATED PROTEIN MIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12897:SF4">
        <property type="entry name" value="REGULATOR OF MON1-CCZ1 COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07035">
        <property type="entry name" value="RMC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21029">
        <property type="entry name" value="RMC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Regulator of MON1-CCZ1 complex N-terminal" evidence="2">
        <location>
            <begin position="28"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Mic1" evidence="1">
        <location>
            <begin position="375"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF99346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="641" mass="71985" checksum="47EE1EAB6A775AB4" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV48</accession>
    <name>A0AAU9FV48_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">PH-sensitive chloride channel 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_07374</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FV48"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099095">
        <property type="term" value="F:ligand-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18987">
        <property type="entry name" value="LGIC_ECD_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19049">
        <property type="entry name" value="LGIC_TM_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000021">
        <property type="entry name" value="glutamate-gated chloride channel isoform X12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000020">
        <property type="entry name" value="glutamate-gated chloride channel isoform X12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="640"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="704"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="986"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="13">
        <location>
            <begin position="449"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="14">
        <location>
            <begin position="647"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="128"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="809"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="890"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="815"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="846"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF99474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1011" mass="112352" checksum="A90ACF1ADFB7A3A0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV59</accession>
    <name>A0AAU9FV59_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_07147</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FV59"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04112">
        <property type="entry name" value="Rab26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000459">
        <property type="entry name" value="ras-related protein Rab-37 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="140"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF99185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="417" mass="45211" checksum="EC30E822250198BF" modified="2024-11-27" version="1">MRRQQTRYRSDSIASPSLSGGRKPSIAAASGSHSPSPLANASPTRRESLVKPTWQHITPGQLPPKTLDKINGINSDDSDDDGYPKRRPSVIVHQRQQSISQQPLLPEFMTASSLFQAQQSPHLSTGNGFMARLSSVRLTSGSEQQPLLDTGGGGGGVNAGGNGNGNGNGNGRAPTVAPPAPGPGSEGASATLCKNAGRALIRMISSGNPPDEDEDFDIMGKVIMLGDSGVGKTSLLIRFRDGRYVPSYFLSTVGIDFRNKVVVVDGTRVKLQIWDTAGQERFRSVTHAYYRDAHALLLLYDVTNKTTYDNIRAWLGEIREYAQEDVVIVLIGNKADCSGSERQVKREDGERLGREHNVPFMETSAKTGLNVELAFTAVARQLKSRGYDHGDDGKFNVHDFVRDNTKARSVCAQCRNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FV88</accession>
    <name>A0AAU9FV88_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD repeat domain phosphoinositide-interacting protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_07407</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FV88"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000664">
        <property type="entry name" value="Autophagy-related 18a, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="394"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="422"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF99519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="456" mass="48182" checksum="72BDF4BB6FF5BEA3" modified="2024-11-27" version="1">MMSLLGRPDVDAGEVFVNFNQNITSLAVATSGGYSLYSLGSVDSTLDKIYHTKSDELFLIERLFESSLVAIVSQRAPRKLKVCHFKKQSEICNYSYANTILAVKLNRERLIVCLEESLYIHNIQDMKVVHTIRDTPCNPLGLCALSSSSEHCYLAYPGSVTSGEVQIFDAINLHAKTMIPAHDTPLAAIAFSPSGTEIATASERGTVIRVFSSQDGSRLFELRRGLKRCVSIVSLSFSNCSEYLVSSSNTETVHIFRLDRSAAETADHGKQSTDDWMGFLSKTVTSYLPTQVTDVFSQGRAFASVSLPEAGVRRMCAIATIQKQLRLLIASQDGYLYVYSIPSIEGAECQLIKRHDLRLEDHYAMDIKVDSPQTLGLNSGVSIGGAAGVPPSVAGAGAGASGSADGKSTATDKPGGSSSSTSGGGGGGASGSGSAGASYASAVKGDEPVAGGPSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6S4</accession>
    <name>A0AAU9G6S4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Larval cuticle protein LCP-30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_03183</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6S4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF200">
        <property type="entry name" value="CUTICULAR PROTEIN 49AB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0193">Cuticle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043818339" evidence="3">
        <location>
            <begin position="24"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="101"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="294"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG04142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="309" mass="35427" checksum="2273F8306704A2FB" modified="2024-11-27" version="1">MFTQSMLRFSAVLVVALIAVCHTSPVPVPVPDEDYYGRSGAVDSLGRYQHMPQPYTHVTDHRELGAYHHVPYPYDGGYGPYAGLNNPYVHDVRPYNHDLYTSTTTTTTKKPTTTTKRTTTSTTTTTTTPRNILFNYDDEGRHKILHQEEIRKKDKYDHAFLTENGIYGEEQAKLHHTGGTHAKGYYEYTGDDGKLYRVNYASNDGGFMPEGDHIPTPPPIPEAIARALKYVEEQHKANGGAQFDHRGFRINHMTKDIKAQIKSMHLERMPKELGDQIRMLQHEVELAEEEEREEERAEQERLERQRLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6T2</accession>
    <name>A0AAU9G6T2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">MAP/microtubule affinity-regulating kinase 3</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Serine/threonine-protein kinase par-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_02833</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Regulates localization and activity of MITF by mediating its phosphorylation, promoting subsequent interaction between MITF and 14-3-3 and retention in the cytosol. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1. Phosphorylates PKP2 and KSR1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with MAPT/TAU. Interacts with DLG5 (via coiled-coil domain). Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5. Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ. Interacts with PKP2 (via N-terminus). Interacts with CDC25C. Interacts with KSR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6T2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14072">
        <property type="entry name" value="STKc_MARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14337">
        <property type="entry name" value="UBA_MARK_Par1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001032">
        <property type="entry name" value="KP78b, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049508">
        <property type="entry name" value="MARK1-4_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="485"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="19">
        <location>
            <begin position="755"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="71"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="287"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="361"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="800"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="926"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1003"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="313"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="397"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="808"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="845"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="926"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="959"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1015"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="514"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFG03630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1067" mass="115754" checksum="CC43AC9BAE6FE952" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6T4</accession>
    <name>A0AAU9G6T4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_02659</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6T4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07132">
        <property type="entry name" value="ALDH_F3AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000003">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="469"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG03387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="498" mass="55609" checksum="8F02B5B623698477" modified="2024-11-27" version="1">MANFDDTLQRARLAFASGKTRNVNFRRKQLENLLRCYEENENDIISALEADLRRPKQESLIVETEFMKNDIKHILYHLSDWVKAEKPSKSIINVMDDVQIYNDPFGVVLVIGAWNYPLQLLLVPVASAIAAGNCVVIKPSEIAGNCAKFIAEVIPKYLDNDCYPVVCGGPSETAELLKQRFDYIFYTGSTRVGKIIHAAANKHLTPTTLELGGKSPCYIDKSVELRTAVKRILWGKLINCGQTCIAPDYILCSKEMQDKFVAEAKDVLKEWYGENIQSSPDLSRVINTNNFQRLIGLMKSGRVAVGGKYDASERYIEPTILVDVKPNDPIMEEEIFGPILPIYNVESAYDAIKFINARESPLVLYIFTSETEVQNLFVNGTQSGGMCVNDTIMHYAVDVLPFGGVGMSGMGSYHGKYGFDTFTHKKSCLGKDLSAVGEKLASARYPPYSDRKGSFLSFLLRKRRPLPNFYLTHVLAVGLGVGLTVLANYYLQGKLLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6U1</accession>
    <name>A0AAU9G6U1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cathepsin propeptide inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_03240</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6U1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043706527" description="Cathepsin propeptide inhibitor domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="3">
        <location>
            <begin position="132"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG04225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="208" mass="23332" checksum="1F976665C89FD11D" modified="2024-11-27" version="1">MNSVALLCVCLMAVAWALPTPNPSPAVPNIEVELPVPDELSFETTATENDWFEATSESSATPQGDILTTEMAPEIVTSEDDSSTALPLEEFTTEGTISEESPQSSQTPTNSEESTTELSAEAKDQLHCLADWDKFLVDFERLYASDIEAEQRRAAFCDNWRKIRKHNKLFDQGLVSYQLGINQFSDQTFKEWLKKQLPDPIFMDEDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6W5</accession>
    <name>A0AAU9G6W5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Proton-coupled folate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_03211</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6W5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17386">
        <property type="entry name" value="MFS_SLC46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507:SF39">
        <property type="entry name" value="GH23453P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507">
        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG04187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="506" mass="55409" checksum="C5E2147255AC95EB" modified="2024-11-27" version="1">MNEEPHAHEKLVAKAARRDPETASSSSAESHQNGEPSAIREPSVAQPLTIYLLEPFILILLFAYNFSSTVLKSQIIYQSCTAGFGYSNEVCLLLGTKNATNETKRIEEEVQPYAARVVVVMRIVECIIPAFCGLFAGAWADRYGRKPLLTCSFLGYGLQYLISAGVAYAAMQTGGMVSPWWYVLSIVPLSCLGSSVTYSVAAVCFLGDVSEGKARSYRMIAYELAIYVGLLLGSFGSGYAYEATNAYVVLCISACSILVALFLMIALLPESLPRSEQNAATPDRDLLALLKDLWSTCSRHRAHKDRSIVFLVMSVLLLAAFVSDGSNSVFYLFMRAKFHWTVKEFTEYESVSILVPAVAGSGGMLFIWSLRQCTSSAVLWLAIVSLLSHSSSSLMKSWATVSWQIYVAIALGVFKSLVNPMCRTIITNLLPAAERGKIFALLGILQTLSPLISSSLYLAIYTRTLDTDPGIFNLLSACLYGLGIVLLVAVWHKKSTNRAYYAPVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6X2</accession>
    <name>A0AAU9G6X2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Putative inorganic phosphate cotransporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_02562</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">May be an inorganic phosphate cotransporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sodium/anion cotransporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6X2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.540:FF:000001">
        <property type="entry name" value="Blast:Putative inorganic phosphate cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000144">
        <property type="entry name" value="Picot, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000003">
        <property type="entry name" value="Solute carrier family 17 member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.540">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027378">
        <property type="entry name" value="Nucleotide_channel_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF280">
        <property type="entry name" value="FI21844P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="348"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFG03264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="512" mass="56687" checksum="F786BB3446A9B038" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6Y3</accession>
    <name>A0AAU9G6Y3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein NDRG3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_02908</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6Y3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000047">
        <property type="entry name" value="protein NDRG3 isoform X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11034">
        <property type="entry name" value="N-MYC DOWNSTREAM REGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="402"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG03718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="470" mass="51899" checksum="D8A841C6B70B0EA3" modified="2024-11-27" version="1">MPSAPTHTAEEAHLLGTMPVDPMDDIELRSVQLHFPNARGSILEACDQRRVPTDKGDIHVAIQGDTSKPAIVTYHDLGLNYATSFAGFFNFPVMRGLLENFCVYHVTAPGQEEGAPALPEDYVYPTMDELAAQLLFVLSHFGLKSVIGFGVGAGANILARFAHGHPDKVGALCLINCVSTQSGWIEWGYQSFNARFLRTKGMTQGVIDYLMWHHFGRNPEERNHDLVQMYKQHFERAVNPTNLAMLINAYIHRNDLHLARTPPGTPGTETAATTLKMPVINITGSLSPHVDDTVTFNGRLDPTNSSWMKISDCALVLEEQPAKLAEAFRLFLQGEGYATPLSTPASSPCGTKYHTYSSIFFANFREQQQQAMEERDREREHDRQRQLNLRIGNRLRETAINCTNNGAGAGNGAGDNNNADASEEEEDQENGNGNGNRAYVTTDTSGTLYYGTQSNKIRITENPLPEPVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6Y8</accession>
    <name>A0AAU9G6Y8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">(S)-2-hydroxy-acid oxidase</fullName>
            <ecNumber evidence="2">1.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_02621</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-hydroxyoctanoate + O2 = 2-oxooctanoate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:67940"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:133514"/>
            <dbReference type="ChEBI" id="CHEBI:176689"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:67941"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:16789"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
            <dbReference type="EC" id="1.1.3.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:16790"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.15" evidence="2"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6Y8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000056">
        <property type="entry name" value="hydroxyacid oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF149">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="290"/>
            <end position="291"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG03335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="345" mass="37456" checksum="9DBB6DDED37BBB64" modified="2024-11-27" version="1">MALVCVEDFEKKAAGQLEKNALDYYRSGAGEQFTLGLNREAFRKLRLRPRCLRDVSRLDIGCPIFDGEVGNARAAGKAGSIFILSTLSTTSLEDLAAGAPDTCKWFQLYIYKDRTITEKLVRRAEKANFKALVLTIDAPIFGHRRADVRNNFSLPSHLTLANFQGVKATGVATTAMGASGINEYVSSQFDPTITWQDIKWLKGITHLPIVVKGVLTAEDAVLAKEFGCAGIIVSNHGARQIDTVPASIEALPEVAKAVGNDLVVMLDGGIMQGNDIFKALALGAKTVFVGRPAVWGLAYNGQKGVEDMLSVLRKDFEITMALIGCQTLNDIQSSMVVHESQYSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6Z2</accession>
    <name>A0AAU9G6Z2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Voltage-dependent calcium channel subunit alpha-2/delta-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_03234</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6Z2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01463">
        <property type="entry name" value="vWA_VGCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000062">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000057">
        <property type="entry name" value="Voltage-dependent calcium channel subunit alpha-2/delta-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051173">
        <property type="entry name" value="Ca_channel_alpha-2/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013680">
        <property type="entry name" value="VDCC_a2/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013608">
        <property type="entry name" value="VWA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166:SF63">
        <property type="entry name" value="STRAIGHTJACKET, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2/DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08473">
        <property type="entry name" value="VGCC_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08399">
        <property type="entry name" value="VWA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043829637" evidence="16">
        <location>
            <begin position="27"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="17">
        <location>
            <begin position="267"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFG04217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1229" mass="142015" checksum="F04DF4916F860F25" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6Z3</accession>
    <name>A0AAU9G6Z3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_03001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6Z3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000080">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF33">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="136"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="701"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="228"/>
            <end position="235"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG03874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="727" mass="79696" checksum="20990781E9BFAB9E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6Z4</accession>
    <name>A0AAU9G6Z4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Heat shock factor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_02701</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6Z4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000027">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
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        <property type="entry name" value="Vert_HSTF_C"/>
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                <person name="Yamamoto D."/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG03733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="822" mass="89152" checksum="0F8C3C7A224C25F7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6Z9</accession>
    <name>A0AAU9G6Z9_DROMD</name>
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        <submittedName>
            <fullName evidence="3">Protein EFR3 homolog cmp44E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_03047</name>
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    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EFR3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6Z9"/>
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        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:protein localization to plasma membrane"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
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        <property type="entry name" value="EFR3-like_ARM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051851">
        <property type="entry name" value="EFR3_Homologs"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN EFR3 HOMOLOG CMP44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12444:SF8">
        <property type="entry name" value="PROTEIN EFR3 HOMOLOG CMP44E"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EFR3_ARM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="666"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG03929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="820" mass="92220" checksum="9E5CEE7D11EF10A2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G743</accession>
    <name>A0AAU9G743_DROMD</name>
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        <submittedName>
            <fullName evidence="11">Patronin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_02937</name>
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    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
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        <subcellularLocation>
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            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
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    <comment type="domain">
        <text evidence="6">The CKK domain binds microtubules.</text>
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    <comment type="similarity">
        <text evidence="6">Belongs to the CAMSAP1 family.</text>
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        <property type="protein sequence ID" value="BFG03778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030507">
        <property type="term" value="F:spectrin binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.360:FF:000002">
        <property type="entry name" value="Patronin, isoform M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.360">
        <property type="entry name" value="CKK domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032940">
        <property type="entry name" value="CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031372">
        <property type="entry name" value="CAMSAP_CC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058042">
        <property type="entry name" value="CAMSAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014797">
        <property type="entry name" value="CKK_CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038209">
        <property type="entry name" value="CKK_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595:SF0">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17095">
        <property type="entry name" value="CAMSAP_CC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08683">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25532">
        <property type="entry name" value="CH_CAMSAP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01051">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51508">
        <property type="entry name" value="CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="157"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="CKK" evidence="10">
        <location>
            <begin position="1502"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="311"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="362"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="526"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="763"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="835"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="935"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1082"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1136"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1276"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1361"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1412"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="641"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="705"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="311"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="418"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="464"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
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        </location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
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            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="978"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1084"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1146"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1156"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1180"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1206"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1249"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
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            <end position="1393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1412"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1440"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1459"/>
            <end position="1477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00841"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG03487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="316" mass="34440" checksum="4FB7F7A08F2297C6" modified="2024-11-27" version="1">MQDPNQQYNYGGGYPPQGGYGGGGYPPQGPPPQGGYPPYAQGGAQPYPQGPYGQPYGQGPPPGGYAPQPGFIQPPPSAGGYGAYDDPESQPKNFSFDDQSIRRGFIRKVYLILMGQLLVTFGAVALFVFHQGTRNFAAKNMWLFWVALGVMLVTMLSMACCESVRRQTPTNFIFLGIFTIAQSFLMGVSATRYAPTEVLLAVGITAAVCLALTLFALQTKYDFTMMGGILIACMVVFLIFGIVAMFMKGKIITLVYASFGALLFSVYLIYDTQLMMGGDHKYSISPEEYIFAALNLYLDIINIFMYILTIIGASRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G752</accession>
    <name>A0AAU9G752_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5'-nucleotidase</fullName>
            <ecNumber evidence="3">3.1.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_02928</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the 5'-nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="3"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G752"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006196">
        <property type="term" value="P:AMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07409">
        <property type="entry name" value="MPP_CD73_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.780.10:FF:000001">
        <property type="entry name" value="NT5E isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000020">
        <property type="entry name" value="NT5E isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="entry name" value="5'-Nucleotidase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008334">
        <property type="entry name" value="5'-Nucleotdase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006146">
        <property type="entry name" value="5'-Nucleotdase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575:SF24">
        <property type="entry name" value="5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575">
        <property type="entry name" value="5'-NUCLEOTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02872">
        <property type="entry name" value="5_nucleotid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01607">
        <property type="entry name" value="APYRASEFAMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00786">
        <property type="entry name" value="5_NUCLEOTIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043095172" description="5'-nucleotidase" evidence="8">
        <location>
            <begin position="25"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="9">
        <location>
            <begin position="40"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="5'-Nucleotidase C-terminal" evidence="10">
        <location>
            <begin position="372"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG03750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="601" mass="65616" checksum="2A9BFCED9139B500" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G758</accession>
    <name>A0AAU9G758_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Patronin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_02937</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The CKK domain binds microtubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CAMSAP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036449">
        <property type="term" value="C:microtubule minus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030507">
        <property type="term" value="F:spectrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.360:FF:000002">
        <property type="entry name" value="Patronin, isoform M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.360">
        <property type="entry name" value="CKK domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032940">
        <property type="entry name" value="CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031372">
        <property type="entry name" value="CAMSAP_CC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058042">
        <property type="entry name" value="CAMSAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014797">
        <property type="entry name" value="CKK_CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038209">
        <property type="entry name" value="CKK_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595:SF0">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17095">
        <property type="entry name" value="CAMSAP_CC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08683">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25532">
        <property type="entry name" value="CH_CAMSAP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01051">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51508">
        <property type="entry name" value="CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="157"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="CKK" evidence="10">
        <location>
            <begin position="1455"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="311"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="362"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="526"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="684"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="711"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="784"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="839"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="884"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1031"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1085"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1225"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1310"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1361"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="640"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="311"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="418"/>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9G786"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07132">
        <property type="entry name" value="ALDH_F3AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000003">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="526"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="10">
        <location>
            <begin position="57"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG03384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="555" mass="61593" checksum="428C666052132F46" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G787</accession>
    <name>A0AAU9G787_DROMD</name>
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        <submittedName>
            <fullName evidence="11">Patronin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_02937</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The CKK domain binds microtubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CAMSAP1 family.</text>
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    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome E"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:calmodulin binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030507">
        <property type="term" value="F:spectrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.360">
        <property type="entry name" value="CKK domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR031372">
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    </dbReference>
    <dbReference type="InterPro" id="IPR058042">
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    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
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    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
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    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014797">
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    </dbReference>
    <dbReference type="InterPro" id="IPR038209">
        <property type="entry name" value="CKK_dom_sf"/>
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    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595:SF0">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17095">
        <property type="entry name" value="CAMSAP_CC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08683">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25532">
        <property type="entry name" value="CH_CAMSAP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01051">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51508">
        <property type="entry name" value="CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="157"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="CKK" evidence="10">
        <location>
            <begin position="1565"/>
            <end position="1699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="311"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="362"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="526"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="835"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="935"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1082"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1136"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1276"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1361"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1412"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1545"/>
            <end position="1567"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="641"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="705"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="311"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="418"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="464"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
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            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
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            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
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            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
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            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
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            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
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            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="938"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="963"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="978"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1084"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1146"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1156"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1180"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1206"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1249"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1412"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1440"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1459"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1478"/>
            <end position="1489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG03783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1706" mass="192442" checksum="49AFB06284FE8729" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G794</accession>
    <name>A0AAU9G794_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ion transport peptide-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_03079</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the arthropod CHH/MIH/GIH/VIH hormone family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G794"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2010.10:FF:000001">
        <property type="entry name" value="Ion transport peptide isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2010.10">
        <property type="entry name" value="Crustacean CHH/MIH/GIH neurohormone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018251">
        <property type="entry name" value="Crust_neurhormone_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031098">
        <property type="entry name" value="Crust_neurohorm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035957">
        <property type="entry name" value="Crust_neurohorm_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001166">
        <property type="entry name" value="Hyperglycemic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35981">
        <property type="entry name" value="ION TRANSPORT PEPTIDE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35981:SF2">
        <property type="entry name" value="ION TRANSPORT PEPTIDE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01147">
        <property type="entry name" value="Crust_neurohorm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00550">
        <property type="entry name" value="HYPRGLYCEMIC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81778">
        <property type="entry name" value="Crustacean CHH/MIH/GIH neurohormone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01250">
        <property type="entry name" value="CHH_MIH_GIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043471084" evidence="8">
        <location>
            <begin position="23"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="40"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="56"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR631098-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG03989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="120" mass="14064" checksum="C304C17B396EECE5" modified="2024-11-27" version="1">MCSRNIKISVVLFLVLIPIFTALPHNHNLSKRSNFFDLECKGIFNKTMFFRLDRICEDCYQLFRETSIHRLCKKDCFDSKWFGECLKVLLIPEEEVSNLQHFLRVVNGSPISFNMTPGQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G799</accession>
    <name>A0AAU9G799_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein painting of fourth</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_02667</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G799"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="213"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="431"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="441"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFG03399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="495" mass="54974" checksum="FC0FB945713509D1" modified="2024-11-27" version="1">MDLKRSPPMPDIGADHKRPRKYHHQENGNEKPAGDGNPLHLGHNLSPCLPGISSAAIGAQPGSWLAESINSDCDTVSLPEFDRNSLYKKDLHGNRLSRREMAKIRKEDSLKCLELEREFVPKSCEDAAAVVLIIRFPDQEITTSMVTGLSPAIRDVGLPSNLEPRYCLAHLKAGADVDTTISDINQVRFGNGFLVAERKAFCEEEEATLIDPWCLYVSNIPFSMTTSAIKGFFAKSFKVDVGVLRREKRARYAFVRYASTELAREAFKELSGTPLHNRTLTVRFRRLKKRPVLLKLPRTTSNVTINTLATDDDDAADVDCKVISPPPLESITISDSDGNCSDSNGAAKEKGKRTSKMSEHELEIQKLKLQMAEYGAIIKTLQAKQEILDMTPKLEPNSYPELCTHVRIPTPVHLMQDIKAECAYLGLPEVTTEPSTTLPTDKLRQDNDPKKAQSLAKSPKPAQDKTKASKATQASPEKDARLEELYAQLETDIDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EQX6</accession>
    <name>A0AAU9EQX6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trimethylguanosine synthase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Cap-specific guanine-N(2) methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Nuclear receptor coactivator 6-interacting protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">PRIP-interacting protein with methyltransferase motif</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_08073</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78479"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="Rhea" id="RHEA-COMP:19089"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:78480"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78507"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="Rhea" id="RHEA-COMP:19090"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:78508"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78471"/>
            <dbReference type="Rhea" id="RHEA-COMP:19085"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:78472"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78475"/>
            <dbReference type="Rhea" id="RHEA-COMP:19086"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:78476"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">May form homooligomers. Interacts with CREBBP/CBP, EED/WAIT1, EP300/P300, NCOA6/PRIP, PPARBP/PBP and SMN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Cajal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the methyltransferase superfamily. Trimethylguanosine synthase family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF89283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9EQX6"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0071164">
        <property type="term" value="F:RNA cap trimethylguanosine synthase activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Trimethylguanosine synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019012">
        <property type="entry name" value="RNA_cap_Gua-N2-MeTrfase"/>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
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        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED"/>
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        <property type="entry name" value="TRIMETHYLGUANOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Methyltransf_15"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
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            <begin position="143"/>
            <end position="172"/>
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    <feature type="region of interest" description="Disordered" evidence="23">
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="162"/>
            <end position="172"/>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
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    <evidence type="ECO:0000256" key="8">
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            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00022691"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    <evidence type="ECO:0000256" key="12">
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        <source>
            <dbReference type="ARBA" id="ARBA00025783"/>
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    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047418"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048740"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048763"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049075"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049790"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00057179"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00064494"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00079339"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00081504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="BFF89283.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="531" mass="61051" checksum="9BDBC6C881334191" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ER89</accession>
    <name>A0AAU9ER89_DROMD</name>
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        <submittedName>
            <fullName evidence="7">Trithorax group protein osa</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_07880</name>
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    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ARID_dom"/>
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        <property type="entry name" value="ARM-type_fold"/>
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        <property type="entry name" value="BAF250/Osa"/>
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        <property type="entry name" value="BRG-1 ASSOCIATED FACTOR 250 BAF250"/>
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        <property type="entry name" value="TRITHORAX GROUP PROTEIN OSA"/>
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    <dbReference type="Pfam" id="PF01388">
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        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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        </location>
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        </location>
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        </location>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
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                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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            <taxon>Diptera</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="periostin isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="FI19380P1"/>
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    <dbReference type="PANTHER" id="PTHR10900">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="4"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <location>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF89924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZI2</accession>
    <name>A0AAU9EZI2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sterile alpha motif domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_10033</name>
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    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
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        <lineage>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Interacts promiscuously (via SAM domain) with EPHA5, EPHA6, EPHA7, EPHA8, EPHB1, EPHB2, EPHB3 and EPHB4 (via SAM domain) (in vitro).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EZI2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd09527">
        <property type="entry name" value="SAM_Samd5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000055">
        <property type="entry name" value="Sterile alpha motif domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051725">
        <property type="entry name" value="SAM-SH3_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12301:SF10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12301">
        <property type="entry name" value="SAM-DOMAIN, SH3 AND NUCLEAR LOCALIZATION SIGNALS PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SAM" evidence="9">
        <location>
            <begin position="7"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="674"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="9">
        <location>
            <begin position="772"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="182"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="606"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="640"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="739"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="855"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="923"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="612"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="657"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="739"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="753"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="855"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="880"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="955"/>
            <end position="979"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00065890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF91832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="979" mass="105744" checksum="0D6E9666CF1BCB12" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZI5</accession>
    <name>A0AAU9EZI5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_07974</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EZI5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032394">
        <property type="entry name" value="Anoct_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF83">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16178">
        <property type="entry name" value="Anoct_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="573"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="616"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="662"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="791"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="932"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin dimerisation" evidence="11">
        <location>
            <begin position="184"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="10">
        <location>
            <begin position="405"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="61"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF89166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="956" mass="109846" checksum="2D3480D38B8DC9AE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZK3</accession>
    <name>A0AAU9EZK3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_08577</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EZK3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14126">
        <property type="entry name" value="STKc_CK1_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000703">
        <property type="entry name" value="Casein kinase I gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022247">
        <property type="entry name" value="Casein_kinase-1_gamma_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12605">
        <property type="entry name" value="CK1gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="21"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="343"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="387"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF89954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="422" mass="48197" checksum="B4DADE7B073DDB0E" modified="2024-11-27" version="1">MYSTRQSVSTTTGVLMVGPNFRVGKKIGCGNFGELRLGKNLYNNEHVAIKMEPMKSKAPQLHLEYRFYKLLGSHAEGVPEVYYFGPCGKYNALVMELLGPSLEDLFDICGRRFTLKSVLLIAIQLLHRIEYVHSRHLIYRDVKPENFLIGRTSTKREKIIHIIDFGLAKEYIDLDTNRHIPYREHKSLTGTARYMSINTHMGREQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRATPIEVLCDGHPEEFATYLRYVRRLDFFETPDYDFLRRLFQDLFERKGYTDEGEFDWTGKTMSTPVGSLQTGHEVIISPNKDRHNVTAKTNAKGGVAAWPDVPKPGATLGNLTPADRHGSVQVVSSTNGELNPDDPTAGHSNTPITQQPEVEVVDETKCCCFFKRKKKKSTRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZK5</accession>
    <name>A0AAU9EZK5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_10058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EZK5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF91862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="562" mass="61093" checksum="946133A9AF2C8FA9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZM3</accession>
    <name>A0AAU9EZM3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tensin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_08614</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EZM3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01213">
        <property type="entry name" value="PTB_tensin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013625">
        <property type="entry name" value="PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033929">
        <property type="entry name" value="Tensin_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051484">
        <property type="entry name" value="Tensin_PTEN_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734:SF10">
        <property type="entry name" value="BLISTERY, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734">
        <property type="entry name" value="TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08416">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="4">
        <location>
            <begin position="18"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF89999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="279" mass="31190" checksum="A5F38A6838230919" modified="2024-11-27" version="1">MEVAADSVRFARSSAKFWYKANLSREDAIALLASARPGTFLVRDSTTYKNAYGLVVRVAQPPAGSQELVRHFLIEPTREGVRLKGCENEPVFTSLSALVFEHSINRLALPCLLRLPEEDIVPGLVATPAQQHLLTHGAATNVLWLYSCDTESLTGNEAIRKAIRLMYAQQKLPEPTEVHFKVTSQGITLTDNTRKKFFRKHYTADVISHCAIDPESRMWTGEGQTEKKTIFAFVARRSHSSKDNQCHVFCDLSVSQPAAAIVSFANRTLPTEKLRNYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZM7</accession>
    <name>A0AAU9EZM7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">BK channel</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Maxi K channel</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_10072</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. Slo sub-subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EZM7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000035">
        <property type="entry name" value="Calcium-activated potassium channel slowpoke"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000005">
        <property type="entry name" value="calcium-activated potassium channel subunit alpha-1 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000015">
        <property type="entry name" value="Calcium-activated potassium channel subunit alpha-1 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01449">
        <property type="entry name" value="BKCHANNELA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16 23" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="8" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="22">
        <location>
            <begin position="356"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="22">
        <location>
            <begin position="852"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="657"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="784"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1192"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="784"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00060897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BFF91892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1222" mass="135730" checksum="AF124BCA9B1B4FB8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZM9</accession>
    <name>A0AAU9EZM9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">THAP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_09000</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EZM9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.20">
        <property type="entry name" value="THAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038441">
        <property type="entry name" value="THAP_Znf_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048366">
        <property type="entry name" value="TNP-like_GBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048365">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21788">
        <property type="entry name" value="TNP-like_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21787">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="100"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF90489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="575" mass="65123" checksum="DA6AC8AED3359981" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZN2</accession>
    <name>A0AAU9EZN2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="13">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_08621</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="13"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000013">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIP12 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIP12 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.50:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIP12 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000018">
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="4 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 18 19" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5 18 19" id="KW-0645">Protease</keyword>
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    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="10 16 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="23">
        <location>
            <begin position="518"/>
            <end position="720"/>
        </location>
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        <location>
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        <location>
            <begin position="953"/>
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        </location>
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        <location>
            <begin position="996"/>
            <end position="1113"/>
        </location>
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    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="1113"/>
            <end position="1153"/>
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            <begin position="1157"/>
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    </feature>
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        <location>
            <begin position="1270"/>
            <end position="1387"/>
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    </feature>
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        <location>
            <begin position="64"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="243"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="388"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
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            <begin position="250"/>
            <end position="260"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="388"/>
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        </location>
    </feature>
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            <begin position="404"/>
            <end position="420"/>
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    </feature>
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            <begin position="424"/>
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    </feature>
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        <location>
            <position position="614"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="613"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        </ligand>
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        <location>
            <position position="617"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        <location>
            <position position="623"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="585"/>
            <end position="586"/>
        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="EMBL" id="BFF91907.1"/>
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    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1455" mass="163966" checksum="CAA6399FAD519A98" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZR1</accession>
    <name>A0AAU9EZR1_DROMD</name>
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        <submittedName>
            <fullName evidence="17">G protein-activated inward rectifier potassium channel 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_10113</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="11">
            <text>K(+)(in) = K(+)(out)</text>
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        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005886">
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        <property type="entry name" value="Putative Inward rectifier potassium channel"/>
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    </dbReference>
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        <property type="entry name" value="INWARDLY RECTIFYING POTASSIUM CHANNEL 2, ISOFORM D"/>
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        <property type="entry name" value="KIRCHANNEL"/>
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        <property type="entry name" value="Voltage-gated potassium channels"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0630">Potassium</keyword>
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    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="220"/>
            <end position="244"/>
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    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="15">
        <location>
            <begin position="103"/>
            <end position="249"/>
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    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="16">
        <location>
            <begin position="256"/>
            <end position="427"/>
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    </feature>
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        <location>
            <begin position="1"/>
            <end position="35"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="10"/>
            <end position="24"/>
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    </feature>
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        <location>
            <begin position="25"/>
            <end position="34"/>
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    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF91952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="456" mass="51903" checksum="79A098B6B170B00D" modified="2024-11-27" version="1">MRFNFSSHSAAPAAATATPATTATNGNEKASATQPLKRAIENDPDSLDSVISNPQSYPITIVPNRPASFYGVAPNGKAFQRQASCRSRNFRPGSMRRVRRRAVFKNGDCNVVQKHLQRRRLRFMQDMYTTMVDWQWRWTLLAFALSFVLSWLFFALIWWLIIYTHGDLEELHMPDNQEESGWAPCVSAIDGFTSCFLFSIETQHTIGYGVRTTSTECPEAIFMMCFQSIYGVMSSAFMAGIVFAKMTRAKQRAQTLLFSKQAVICQRDGSLSLMFRVGDMRKSHIIGAGVRAQLIRTKSTKEGEVMTQFFTELEIGSDDSGSDLFFIWPMIIEHKIDENSPLYNLNATDMLHDRFEIVVILEGTVESTGQSTQARSSYINTEILWGHRFDPVVLYNKDLQAYEIDYARFNETTQVDTPLCSARELNEIYKIQEGFRTPETTFTMRQLSHNHSDQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F2X5</accession>
    <name>A0AAU9F2X5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Dedicator of cytokinesis protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_08962</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08695">
        <property type="entry name" value="C2_Dock-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11872">
        <property type="entry name" value="SH3_DOCK_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.740:FF:000006">
        <property type="entry name" value="Blast:Dedicator of cytokinesis protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.410:FF:000003">
        <property type="entry name" value="Dedicator of cytokinesis protein 4"/>
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    </dbReference>
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        <property type="entry name" value="Dedicator of cytokinesis protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="C2 domain"/>
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    </dbReference>
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        <property type="entry name" value="Dedicator of cytokinesis N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ARM-type_fold"/>
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    <dbReference type="InterPro" id="IPR037811">
        <property type="entry name" value="C2_Dock-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
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        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032376">
        <property type="entry name" value="DOCK_N"/>
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        <property type="entry name" value="DOCK_N_sub1"/>
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    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056372">
        <property type="entry name" value="TPR_DOCK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653:SF12">
        <property type="entry name" value="SPONGE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16172">
        <property type="entry name" value="DOCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23554">
        <property type="entry name" value="TPR_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="9">
        <location>
            <begin position="5"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="C2 DOCK-type" evidence="10">
        <location>
            <begin position="419"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="11">
        <location>
            <begin position="1213"/>
            <end position="1624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1616"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1703"/>
            <end position="1761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1769"/>
            <end position="1788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1892"/>
            <end position="1922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1969"/>
            <end position="1991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2020"/>
            <end position="2048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1641"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1892"/>
            <end position="1901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="2020"/>
            <end position="2040"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF90439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2048" mass="231130" checksum="54B055766CF32F27" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F2X6</accession>
    <name>A0AAU9F2X6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_09437</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F2X6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20993">
        <property type="entry name" value="GH07914P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20993:SF0">
        <property type="entry name" value="GH07914P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044713104" evidence="1">
        <location>
            <begin position="19"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF91071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="259" mass="29036" checksum="B4539A8177E529EF" modified="2024-11-27" version="1">MAFQLFLVALLGIALVSATPVPQQAGSANFTSAMTRYLQAFRSVMPCGYAPEGIPVLAPIVTPFYAYDISTDDYSLKGNVSNMRIEGLNNFKILSASDYGKTGQAAYDVIFPKIQILGASEVEGFVNIGGFHLPIRQRDVINEALVDLRFVGSYTFANSLTNSSGLRIVDFKQSIYLADVLMDNWNSLWNISTNNFWNRYSRIFISLGLEEIQPKITDFLYQNYFVPKINELLANVSMEELVNFYQSQAQLWENAHCQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F2Z4</accession>
    <name>A0AAU9F2Z4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Clathrin interactor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_08999</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F2Z4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF90484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="99" mass="10321" checksum="329470D2A5B8F3D1" modified="2024-11-27" version="1">MSKAGKSNAPALSMNALKTQSPAKTQAPLNVQTTGGFSGLSPLTSPNLFGAQQPQSNIPQNQAAFANFGAFQQQPSQNHSNNNNNVNNNGSASAFDLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F381</accession>
    <name>A0AAU9F381_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">C-terminal-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_09173</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F381"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001221">
        <property type="term" value="F:transcription coregulator binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05299">
        <property type="entry name" value="CtBP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000012">
        <property type="entry name" value="C-terminal-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043322">
        <property type="entry name" value="CtBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051638">
        <property type="entry name" value="CTBP_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029:SF7">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="7">
        <location>
            <begin position="38"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="8">
        <location>
            <begin position="134"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="388"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="392"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF90694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="492" mass="52383" checksum="DFC82C2FF496153C" modified="2024-11-27" version="1">MDKNLMMPKRSRIDVKGSFANGPLQARPLVALLDGRDCSIEMPILKDVATVAFCDAQSTSEIHEKVLNEAVGALMWHTIILTKEDLEKFKALRIIVRIGSGTDNIDVKAAGELGIAVCNVPGYGVEEVADTTMCLILNLYRRTYWLANMVREGKKFTGPEQVREAAHGCARIRGDTLGLVGLGRIGSAVALRAKAFGFNVIFYDPYLPDGIDKSLGLTRVYTLQDLLFQSDCVSLHCTLNEHNHHLINEFTIKQMRPGAFLVNTARGGLVDDETLALALKQGRIRAAALDVHENEPYNVFQGALKDAPNLICTPHAAFFSDASATELREMAATEIRRAIVGNIPDVLRNCVNKEYFMRTPPTAAAGGVAAAVYPEGLNGGCYTGALHHRAHSTTPHDGPHSTTNIGSSSSSSALAQPPPPPNSVAAAAAAVAAAAAALLPSPVPPTVVPTVPHLSPQVGGLPLGIPLSAPDPNNHLLSSIKAEVKAESTETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F390</accession>
    <name>A0AAU9F390_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_07832</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase gamma subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F390"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04618">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04641">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000003">
        <property type="entry name" value="Protein kinase AMP-activated non-catalytic subunit gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF35">
        <property type="entry name" value="LD22662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="450"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="529"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="604"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="675"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="60"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="255"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="761"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="801"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="837"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="863"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="877"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="916"/>
            <end position="930"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF88970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="941" mass="102822" checksum="9FB8CBF94F59D198" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F393</accession>
    <name>A0AAU9F393_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glucosamine 6-phosphate N-acetyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_09660</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>D-glucosamine 6-phosphate + acetyl-CoA = N-acetyl-D-glucosamine 6-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="2.3.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acetyltransferase family. GNA1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.4" evidence="6"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F393"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004343">
        <property type="term" value="F:glucosamine 6-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000043">
        <property type="entry name" value="Glucosamine 6-phosphate N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039143">
        <property type="entry name" value="GNPNAT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355:SF11">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="7">
        <location>
            <begin position="38"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF91356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="224" mass="25130" checksum="4892FE52797DB4F6" modified="2024-11-27" version="1">MQETYLYDPSLLLKLDFHRTPATFKPFISATNPGEPWMKVRPLKDTDYDRGFLQLLSQLTHVGNVNRTQFLTRFSQMKASGDYFVTVIEDTRKNEIIGAASLVIERKFIHNCSVRGRLEDVVVNDTYRGKQLGKLIVVTVSLLAEELGCYKMSLDCKDKLIKFYESLGYVLLPGNSNSMTIRYDEGTTLKRNTTSAMAGSSSAAGGDSCQTVVVILPFKSTFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F3B8</accession>
    <name>A0AAU9F3B8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Gamma-aminobutyric acid type B receptor subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">G-protein coupled receptor 51</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_09701</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F3B8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="term" value="C:G protein-coupled receptor heterodimeric complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004965">
        <property type="term" value="F:G protein-coupled GABA receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15294">
        <property type="entry name" value="7tmC_GABA-B-R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06366">
        <property type="entry name" value="PBP1_GABAb_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000063">
        <property type="entry name" value="Gamma-aminobutyric acid type B receptor subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000072">
        <property type="entry name" value="Gamma-aminobutyric acid type B receptor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002455">
        <property type="entry name" value="GPCR3_GABA-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519">
        <property type="entry name" value="GABA-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519:SF74">
        <property type="entry name" value="GAMMA-AMINOBUTYRIC ACID TYPE B RECEPTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01177">
        <property type="entry name" value="GABAB1RECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01176">
        <property type="entry name" value="GABABRECEPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12 23" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 21" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043695231" description="Gamma-aminobutyric acid type B receptor subunit 2" evidence="21">
        <location>
            <begin position="23"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="487"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="562"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="620"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="661"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="684"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="22">
        <location>
            <begin position="446"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="920"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1056"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="938"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="964"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="991"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1085"/>
            <end position="1095"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BFF91416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1227" mass="139017" checksum="75833502F104C5FE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F3C3</accession>
    <name>A0AAU9F3C3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of very long chain fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Very-long-chain 3-oxoacyl-CoA synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_09258</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="10"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F3C3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF69">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="240"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF90799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="344" mass="40110" checksum="A18F4E5E9863963E" modified="2024-11-27" version="1">MHPHRRQGYRDYLTRFYDGWRDLMDNKSDPRTQGYPLMSSPFPTIAISLTYAYIVKVLGPKLMENRKPFELRKVLIVYNAAQVIFSTWLFYESCIGGWLNGYNLRCEPVDYSYSPKAIRTAEGCWWYYFSKFTEFFDTFFFVMRKRYDQVSTLHVIHHGIMPVSVWWGVKFTPGGHSTFFGFLNTFVHIIMYTYYMLAAMGPKMQRYLWWKKYLTVLQMIQFVLVMVHSFQLFFKNDCNYPIGFAYFIGAHAVMFYFLFSNFYKRAYVKRNAKEKAAALKANGHANGAIKTQKDGNVPESTGNGKLNGSANGFHNTFSKFTAEMCNPALNTTTRQRALVNAGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCY6</accession>
    <name>A0AAU9FCY6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Trissin receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_11346</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FCY6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15012">
        <property type="entry name" value="7tmA_Trissin_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000304">
        <property type="entry name" value="Trissin receptor, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000425">
        <property type="entry name" value="Trissin receptor, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF194">
        <property type="entry name" value="TRISSIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 8 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="237"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="317"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="581"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="621"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="217"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="485"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="539"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="663"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="489"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="549"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="681"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF93500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="697" mass="76829" checksum="F8152993D4A640B2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCZ3</accession>
    <name>A0AAU9FCZ3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_11444</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 24">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
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    </dbReference>
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        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
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        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
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    <dbReference type="FunFam" id="3.90.190.10:FF:000001">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase F isoform A"/>
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        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
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        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X4"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Uncharacterized protein, isoform B"/>
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    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="11"/>
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    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="FN3_dom"/>
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        <property type="entry name" value="FN3_sf"/>
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    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
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    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
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    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
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    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
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    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
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    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
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    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
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    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF6">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
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        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="FNTYPEIII"/>
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    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
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    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043964489" description="protein-tyrosine-phosphatase" evidence="17">
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            <begin position="32"/>
            <end position="1935"/>
        </location>
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        <location>
            <begin position="35"/>
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    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="139"/>
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    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="233"/>
            <end position="315"/>
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    </feature>
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        <location>
            <begin position="323"/>
            <end position="413"/>
        </location>
    </feature>
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        <location>
            <begin position="423"/>
            <end position="517"/>
        </location>
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        <location>
            <begin position="521"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="616"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="720"/>
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        </location>
    </feature>
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        <location>
            <begin position="817"/>
            <end position="910"/>
        </location>
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        <location>
            <begin position="914"/>
            <end position="1007"/>
        </location>
    </feature>
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        <location>
            <begin position="1009"/>
            <end position="1111"/>
        </location>
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        <location>
            <begin position="1379"/>
            <end position="1634"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="19">
        <location>
            <begin position="1551"/>
            <end position="1625"/>
        </location>
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    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="18">
        <location>
            <begin position="1666"/>
            <end position="1925"/>
        </location>
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    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="19">
        <location>
            <begin position="1843"/>
            <end position="1916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="598"/>
            <end position="627"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1251"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1251"/>
            <end position="1263"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00010504"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50056"/>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93626.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1935" mass="218279" checksum="8ED4414EB2452676" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCZ4</accession>
    <name>A0AAU9FCZ4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cyclin-dependent kinase-like 2</fullName>
            <ecNumber evidence="4">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_11364</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF93522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FCZ4"/>
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        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07847">
        <property type="entry name" value="STKc_CDKL1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cyclin-dependent kinase-like 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000261">
        <property type="entry name" value="cyclin-dependent kinase-like 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
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        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CYCLIN-DEPENDENT KINASE-LIKE 1"/>
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    </dbReference>
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        <property type="entry name" value="Pkinase"/>
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    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15 16" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="8 15 16" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="6 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="22"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="362"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="366"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00039642"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
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            <dbReference type="ARBA" id="ARBA00048367"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000304"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="410" mass="47110" checksum="68910089F0E1E83C" modified="2024-11-27" version="1">MFEISKLFSLRRPQGSSKMDRYEKLSRLGEGSYGVVYKCRDRETGALVAVKRFVESEDDPAIRKIALREIRLLKNLKHPNLVSLLEVFRRKRRLHLVFEFCELTVLHELERHPQGCPEHLTKQICYQTLLGVAYCHKQGCLHRDIKPENILLTAQGQVKLCDFGFARMLSPGENYTDYVATRWYRAPELLVGDTQYGTPVDVWAIGCLFAELVRGEALWPGRSDVDQLYLIRKTLGDLLPRHIQIFGQNEYFKGITLPVPPTLEPLEDKMPPKSLQNPLTIDFLKKCLDKDPVKRWSCEKLIKHSYFDDYIAKQREQEHVNSMEAATLRQQQLASQQFMLATAAQQLQTGAAQAAAIAATRDKSKTSNTSLPLLTGSQHHHHQHQDYMKLQPLNKNGNLLHRTEHHLPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FD96</accession>
    <name>A0AAU9FD96_DROMD</name>
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        <submittedName>
            <fullName evidence="5">Protein numb</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="ORF">DMAD_11474</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="PTB_Numb"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="protein numb isoform X1"/>
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    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Numb/numb-like"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47368">
        <property type="entry name" value="NUMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47368:SF2">
        <property type="entry name" value="PID DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06311">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <location>
            <begin position="81"/>
            <end position="208"/>
        </location>
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        <location>
            <begin position="1"/>
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            <begin position="408"/>
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        </location>
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            <begin position="498"/>
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            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
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            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="536" mass="57967" checksum="6E3F5532C3E700D2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FD98</accession>
    <name>A0AAU9FD98_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Condensin complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_11414</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the CND2 (condensin subunit 2) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FD98"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022816">
        <property type="entry name" value="Condensin_barren_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108:SF9">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05786">
        <property type="entry name" value="Cnd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017126">
        <property type="entry name" value="Condensin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="8 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="113"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="275"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="412"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="414"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR017126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF93584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="715" mass="79477" checksum="837915CEB6FE688B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDC5</accession>
    <name>A0AAU9FDC5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Homeobox protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_11494</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="556"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1405"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1775"/>
            <end position="1788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="2366"/>
            <end position="2383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="2479"/>
            <end position="2495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="2501"/>
            <end position="2514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="2910"/>
            <end position="2919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="2931"/>
            <end position="2940"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF96505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2940" mass="332467" checksum="F3D0E860FADD73A8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLJ6</accession>
    <name>A0AAU9FLJ6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_05126</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLJ6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046824">
        <property type="entry name" value="Mss51-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032717">
        <property type="entry name" value="Mss51_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28069">
        <property type="entry name" value="GH20023P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28069:SF2">
        <property type="entry name" value="GH20023P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20179">
        <property type="entry name" value="MSS51_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13824">
        <property type="entry name" value="zf-Mss51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="168"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="53"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF96492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="578" mass="66368" checksum="159E2A3F5FAC9645" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLK1</accession>
    <name>A0AAU9FLK1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Alanine--glyoxylate aminotransferase 2, mitochondrial</fullName>
            <ecNumber evidence="28">2.6.1.18</ecNumber>
            <ecNumber evidence="12">2.6.1.40</ecNumber>
            <ecNumber evidence="5">2.6.1.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">(R)-3-amino-2-methylpropionate--pyruvate transaminase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Beta-ALAAT II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Beta-alanine-pyruvate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">D-3-aminoisobutyrate-pyruvate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">D-AIBAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">D-beta-aminoisobutyrate-pyruvate aminotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="41" type="ORF">DMAD_05357</name>
    </gene>
    <organism evidence="41 42">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="41 42" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="41 42">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="38">Multifunctional aminotransferase with a broad substrate specificity. Catalyzes the conversion of glyoxylate to glycine using alanine as the amino donor. Catalyzes metabolism of not L- but the D-isomer of D-beta-aminoisobutyric acid to generate 2-methyl-3-oxopropanoate and alanine. Catalyzes the transfer of the amino group from beta-alanine to pyruvate to yield L-alanine and 3-oxopropanoate. Can metabolize NG-monomethyl-L-arginine (NMMA), asymmetric NG,NG-dimethyl-L-arginine (ADMA) and symmetric NG,N'G-dimethyl-L-arginine (SDMA). ADMA is a potent inhibitor of nitric-oxide (NO) synthase, and this activity provides mechanism through which the kidney regulates blood pressure.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(2S)-2-aminobutanoate + glyoxylate = 2-oxobutanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77339"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:74359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(R)-3-amino-2-methylpropanoate + pyruvate = 2-methyl-3-oxopropanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:18393"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:57731"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.40"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:18394"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>2-oxobutanoate + L-alanine = (2S)-2-aminobutanoate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:77355"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:74359"/>
            <dbReference type="EC" id="2.6.1.44"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>2-oxohexanoate + N(omega),N(omega)-dimethyl-L-arginine = L-2-aminohexanoate + 5-(3,3-dimethylguanidino)-2-oxopentanoate</text>
            <dbReference type="Rhea" id="RHEA:77363"/>
            <dbReference type="ChEBI" id="CHEBI:35177"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:58455"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>2-oxopentanoate + N(omega),N(omega)-dimethyl-L-arginine = 5-(3,3-dimethylguanidino)-2-oxopentanoate + L-2-aminopentanoate</text>
            <dbReference type="Rhea" id="RHEA:77359"/>
            <dbReference type="ChEBI" id="CHEBI:28644"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:58441"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>3-oxopropanoate + L-alanine = beta-alanine + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:14077"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:33190"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.18"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="26">
            <dbReference type="Rhea" id="RHEA:14079"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>L-ornithine + glyoxylate = 5-amino-2-oxopentanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77331"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58802"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-ornithine + pyruvate = 5-amino-2-oxopentanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:77327"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58802"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>N(omega),N('omega)-dimethyl-L-arginine + glyoxylate = 5-(3,3'-dimethylguanidino)-2-oxopentanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77315"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:197308"/>
            <dbReference type="ChEBI" id="CHEBI:197310"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>N(omega),N('omega)-dimethyl-L-arginine + pyruvate = 5-(3,3'-dimethylguanidino)-2-oxopentanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:77307"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:197308"/>
            <dbReference type="ChEBI" id="CHEBI:197310"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>N(omega),N(omega)-dimethyl-L-arginine + 2-oxobutanoate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + (2S)-2-aminobutanoate</text>
            <dbReference type="Rhea" id="RHEA:77351"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:74359"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>N(omega),N(omega)-dimethyl-L-arginine + glyoxylate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77311"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>N(omega),N(omega)-dimethyl-L-arginine + oxaloacetate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + L-aspartate</text>
            <dbReference type="Rhea" id="RHEA:77343"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>N(omega),N(omega)-dimethyl-L-arginine + pyruvate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:77303"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77323"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:114953"/>
            <dbReference type="ChEBI" id="CHEBI:197314"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:77319"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:114953"/>
            <dbReference type="ChEBI" id="CHEBI:197314"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>glyoxylate + L-alanine = glycine + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:24248"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.44"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24249"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>oxaloacetate + L-alanine = L-aspartate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:77347"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 39">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
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        <property type="protein sequence ID" value="BFF96806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome J"/>
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        <property type="term" value="C:mitochondrion"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0047305">
        <property type="term" value="F:(R)-3-amino-2-methylpropionate-pyruvate transaminase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:beta-alanine:pyruvate transaminase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:pyridoxal phosphate binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:glyoxylate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:L-alanine catabolic process, by transamination"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="OAT_like"/>
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        <property type="entry name" value="Alanine--glyoxylate aminotransferase 2, mitochondrial"/>
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    </dbReference>
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        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
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        <property type="entry name" value="Aminotran_3"/>
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        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
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        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
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    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
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            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
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            <dbReference type="ARBA" id="ARBA00033660"/>
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            <dbReference type="ARBA" id="ARBA00043669"/>
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            <dbReference type="ARBA" id="ARBA00043726"/>
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            <dbReference type="ARBA" id="ARBA00043751"/>
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            <dbReference type="ARBA" id="ARBA00043758"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="EMBL" id="BFF96806.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="511" mass="56103" checksum="60EEAB1EEB6A7CBC" modified="2024-11-27" version="1">MMSKRLLAHTRRVMPSSSSSRFSSSTTLHSKAAPAVMNLVQSEMPETGHRPSGYVGPSYEQILETRKTHLTPNLVAHFKKPLVIHEGHMQWLYDHEGRRYLDMFGGIVTVSVGHCHPKVNQALSEQISKLWHTTNIYMHPKIHEYAERLVAKFPGNLKSVCFVNSGSEANDLAMLMARVHTGNQDILSFRNAYHGMSPYTMGLTAHSTWRFPLAGVNNGLVHVMNPDPYQGIWGGAACRDSPVQTTRSCQCQEGGGCQAGSAYYNELEETFKYSLPRGKVAAMFAESIQGVGGTVQFPKGYLKRAAALVRANGGLFVADEVQTGFGRTGEHFWGFEGHDYVPDIVTMAKGIGNGFPLAAVVTTPEIAASLGQALHFNTYGGNPMASAVGIAVLDVIEEEQLQRNSLEVGTYFLKCLEELQQRFEIVGDVRGKGLMIGVELVEDCEKRTPLATPHVLEIWETCKDLGVLFGRGGLHGNVLRIKPPMCIDRSDVKFAVDVLDRAITASLAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLK4</accession>
    <name>A0AAU9FLK4_DROMD</name>
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        <recommendedName>
            <fullName evidence="5">Cornetto</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">DMAD_05312</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <location>
            <begin position="1"/>
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        </location>
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        </location>
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            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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        </location>
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            <begin position="754"/>
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        </location>
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            <begin position="770"/>
            <end position="780"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF96737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1135" mass="124225" checksum="01DAC2A9AD8C0690" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLK5</accession>
    <name>A0AAU9FLK5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein melted</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05313</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MELT/VEPH family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF96739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PH_MELT_VEPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ventricular zone-expressed PH domain-containing protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039888">
        <property type="entry name" value="Melted-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
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</entry>
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            <authorList>
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                <person name="Yamamoto D."/>
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        <source>
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</entry>
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    <accession>A0AAU9FLP0</accession>
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            <taxon>Insecta</taxon>
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        </lineage>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12 13">RF1</strain>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="11">
        <location>
            <begin position="1"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF96498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="453" mass="50512" checksum="A08240EA1AA3ED60" modified="2024-11-27" version="1">MFATQIGPTGLVKGFLRPETAQGIFVNFKRLLEFNQGKLPFAVAQIGNSFRNEISPRSGLIRVREFTMAEIEHFCDPTQKDHPKFGNVKDEKMTLYSACNQMDGKSATQFAIGAAVSAKLVANETLGYYMARIQQFLLAIGIKPECLRFRQHMSNEMAHYACDCWDAEILTSYGWVECVGCADRSAYDLGQHTAATGVRLVAEKRLPAPKTVEVSEIVPNKQALGKTFKKDAKTITEALAKLSLEEISRAEQELNANGQYKLILADGNTHELAKDTISVKHSSKTVHVEEFIPSVVEPSFGIGRIMYALLEHSFQCREGDDQRCYFTLPPLVAPLKCSILPLSNNAEFQPFTQKLSSFLTKAELSHKVDDSSGSIGRRYARTDEIAIPYGITVDFDTLKEPHTVTLRDRNSMKQVRVGLEKVVGVVKDLATARTSWEQVVAQYPVFEQQEATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLP1</accession>
    <name>A0AAU9FLP1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">triacylglycerol lipase</fullName>
            <ecNumber evidence="1">3.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05156</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="1"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLP1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07218">
        <property type="entry name" value="Pat_iPLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000003">
        <property type="entry name" value="Patatin-like phospholipase domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033562">
        <property type="entry name" value="PLPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12406:SF41">
        <property type="entry name" value="BRUMMER, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12406">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2 IPLA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="6">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="446"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="4">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF96537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="497" mass="56826" checksum="38D1BDDDBA2323D5" modified="2024-11-27" version="1">MTSDFFRVVNEARRYSLGPFSPSFNIQTCLLEGLQKHLPDDAHKRVNGRLHISLTRVYDGKNVIISEFESREEVLQALLCACFIPGFSGILPPRFRGVRYMDGAFSDNLPILDENTITVSPFCGESDICPRDQSSQLFHLNWANTSIEISRQNINRFVRILFPPRPEFLSKFCQQGFDDALQFLHRNNLINCRRCVAVQSTFVVSETVAQPQEFDPECRECKKHRKDALSSNMPQTVLDVIQDYIEQANKGLANWIFKHRGIKLLSLPATVPMDFFLATISKISTLTPKLTKQARQMVEELINQLNNAMQLRLLNKFTLYDQPHFDATGLLHSNRLYGPRVSILVDECGATPLEDDVDNFEHVLKMTTHNDALYAYYYTDNNTNKVKVTEIFDFDDMTEHDELATDLQIYEGSVEDHPNPHQHSHNTVVSEWNGVESDFFIDAQTPNVDVDAPKPHTSRFPQTDFEAESDASVLSDPEADQSFRPSTQSNDMYIEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLP5</accession>
    <name>A0AAU9FLP5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pancreatic lipase-related protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_05404</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLP5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF173">
        <property type="entry name" value="LIPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Lipase" evidence="5">
        <location>
            <begin position="17"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF96866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="282" mass="30595" checksum="49104F8488F5748A" modified="2024-11-27" version="1">MVLASLGSAATGVNGLVGFLLHTRRIQESPQNLEPEVESLVRSSFYAADPVVLSVPRWLGNKSSPEHSAVVAAQLSQRECNVITVDLAETTNEAAITESVGNLIELLHRQFDVPLERILLVGFAEGAHLAGAVAAKVGSDLGQRFPHLTALDPSEASLEHVLSPSDAQFVEVVHTNGGGLGTLERLGHVDYYPNGGETQPGCVEDSCSHERSFELLEEMWSHENDFVSALCGSVETMSVQNCRWVSLKMGQRSQREPTPGIYFLETRSSQPFARGAYHISFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLP6</accession>
    <name>A0AAU9FLP6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcription factor Sox-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05196</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
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            <authorList>
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                <person name="Yamamoto D."/>
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</entry>
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                <person name="Yamamoto D."/>
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        </source>
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        <source>
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        </source>
    </evidence>
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</entry>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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    </evidence>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="1038" mass="109426" checksum="1C8D18D561798172" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FM07</accession>
    <name>A0AAU9FM07_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Poly(RC)-binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_05451</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FM07"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22438">
        <property type="entry name" value="KH-I_PCBP_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02396">
        <property type="entry name" value="KH-I_PCBP_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22439">
        <property type="entry name" value="KH-I_PCBP_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000003">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="22"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="101"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="313"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="547"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="574"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF96932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="593" mass="59669" checksum="F5613EEA02B8B056" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FM10</accession>
    <name>A0AAU9FM10_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DMAD_05270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FM10"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF96685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="77" mass="8673" checksum="B8F93AC4029DAA99" modified="2024-11-27" version="1">MAPEAGDKSGAAARLYCGMGNHFLKTPSTIIRDPKMLRFARYINTELRYDTKICLNCFKILGQLYKFKAVEDHSVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FM11</accession>
    <name>A0AAU9FM11_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Adenosine kinase</fullName>
            <shortName evidence="10">AK</shortName>
            <ecNumber evidence="3 10">2.7.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Adenosine 5'-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_05251</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>adenosine + ATP = AMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20824"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 3 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.20" evidence="3 10"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FM11"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004001">
        <property type="term" value="F:adenosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01168">
        <property type="entry name" value="adenosine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000006">
        <property type="entry name" value="Adenosine kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001805">
        <property type="entry name" value="Adenokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769:SF3">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00989">
        <property type="entry name" value="ADENOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 10" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="11">
        <location>
            <begin position="37"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601805-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF96660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="345" mass="37790" checksum="FFB8D6E45FAEC4FE" modified="2024-11-27" version="1">MTSTLQEGILVGCGNPLLDISASVPMDFLKKYEMNEDDAILAEERHMPIYRELVDGFQAEFLAGGSVQNSLRIAQWILGQPKVAVFFGCVGEDEYADILMEKARSAGLDVHYQIKKDVPTGTCAVLITGTHRSLCANLAAANNFTIDHLDQPHNKTLVENALYYYISGFFLTVNPPSIMQVAATALAKQRPFLMNLSAPFISQFYMAPLLAVMPYVDIIFGNEAEAHAFATAQGWPTEDLREIGKRLVALDKLNPARPRIAILTQGCDPVLLIQRDSVEEFPVTRLAVHEIVDTNGAGDAFVGGFLSQFVQGKSLDVCIRCGNYAAGHIIKNPGCTYTGEPQFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FM12</accession>
    <name>A0AAU9FM12_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_05502</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="228" mass="24578" checksum="FCF8194FE20BC226" modified="2024-11-27" version="1">MSGGMDKRKLSTSGDSLYEILGLPKTATGDDIKKTYRKLALKYHPDKNPDNVDASDKFKEVNRAHSILSDQTKRNIYDNYGSLGLYIAEQFGEENVNAYFVVTSPAVKAVFICCAVVTGCCCCCCCCCCCNFCCGKFKPPVNESHDQYAHLNEDVDLDDVNLGAGGGPVTSQPREQPGGQPVFAMPPPTGAAVGVNPFTGAPVAANENTSLNTTEQTTYTPDMVNQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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            <strain evidence="5 6">RF1</strain>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
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        <location>
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        <location>
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    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002668">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="8"/>
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        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Immunoglobulin_dom"/>
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    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF155">
        <property type="entry name" value="IG-LIKE AND FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN C25G4.10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="4"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF13927">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="4"/>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043829625" evidence="4">
        <location>
            <begin position="22"/>
            <end position="1651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1479"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="24"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="6">
        <location>
            <begin position="285"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="6">
        <location>
            <begin position="525"/>
            <end position="616"/>
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    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="6">
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            <begin position="935"/>
            <end position="1026"/>
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        <location>
            <begin position="1117"/>
            <end position="1216"/>
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    </feature>
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        <location>
            <begin position="1324"/>
            <end position="1428"/>
        </location>
    </feature>
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            <begin position="715"/>
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        <location>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF96531.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1651" mass="177746" checksum="4ABFA5139A32D5A0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FM25</accession>
    <name>A0AAU9FM25_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="13">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_05281</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19714"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(E)-hexadec-2-enoate + ATP + CoA = (2E)-hexadecenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36139"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
            <dbReference type="ChEBI" id="CHEBI:72745"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:36140"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + ATP + CoA = 12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52112"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:90718"/>
            <dbReference type="ChEBI" id="CHEBI:136408"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:52113"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + ATP + CoA = 15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:78832"/>
            <dbReference type="ChEBI" id="CHEBI:136409"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:52117"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = 5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65341"/>
            <dbReference type="ChEBI" id="CHEBI:136407"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:52109"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hexadecanoate + ATP + CoA = hexadecanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:30751"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:30752"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="13"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FM25"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047676">
        <property type="term" value="F:arachidonate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05927">
        <property type="entry name" value="LC-FACS_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045311">
        <property type="entry name" value="LC-FACS_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF107">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 13 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="14">
        <location>
            <begin position="89"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF96700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="666" mass="74612" checksum="EBDAE12414092955" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FM52</accession>
    <name>A0AAU9FM52_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxisomal ATPase PEX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Peroxin-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_05200</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with PEX6; forming the PEX1-PEX6 AAA ATPase complex, which is composed of a heterohexamer formed by a trimer of PEX1-PEX6 dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FM52"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19526">
        <property type="entry name" value="RecA-like_PEX1_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000149">
        <property type="entry name" value="Nuclear valosin-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000105">
        <property type="entry name" value="PeRoXisome assembly factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015342">
        <property type="entry name" value="PEX1-N_C-lobe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF12">
        <property type="entry name" value="PEROXISOMAL ATPASE PEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09262">
        <property type="entry name" value="PEX-1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="19">
        <location>
            <begin position="478"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="19">
        <location>
            <begin position="755"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="175"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00064205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF96601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="970" mass="109479" checksum="FE485610582A6A2D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FM60</accession>
    <name>A0AAU9FM60_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Neurexin-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_05334</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the neurexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FM60"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000016">
        <property type="entry name" value="Contactin-associated protein-like 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000242">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003585">
        <property type="entry name" value="Neurexin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF91">
        <property type="entry name" value="NEUREXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00294">
        <property type="entry name" value="4.1m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043639215" evidence="13">
        <location>
            <begin position="31"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1213"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="14">
        <location>
            <begin position="42"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="184"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="370"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="537"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="791"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="958"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="998"/>
            <end position="1178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF96772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1279" mass="144596" checksum="73705752AFA13DFC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FM69</accession>
    <name>A0AAU9FM69_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Poly(RC)-binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_05451</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FM69"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22438">
        <property type="entry name" value="KH-I_PCBP_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02396">
        <property type="entry name" value="KH-I_PCBP_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22439">
        <property type="entry name" value="KH-I_PCBP_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000003">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="22"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="105"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="318"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF96923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="416" mass="43652" checksum="6E626863BB2B261B" modified="2024-11-27" version="1">MEDNNTSSSAGGTSIKHEDPSVTLTIRLIMQGKEVGSIIGKKGEIVNRFREESGAKINISDGSCPERIVTVSGTTNAIFSAFTLITKKFEEWCSQFNDAGKIGKTQIPIRLIVPASQCGSLIGKSGSKIKEIRQTTGCSIQVASEMLPNSTERAVTLSGSAEQITQCIYQICLVMLESPPRGATIPYRPKPQVTGPVILANGQAFTIQGNYAVPTQETCPVFPLALATGGLHAGISGLADPLLKGAHLQGAVPAHHHHLQQMPDQVAKNPLASLAALGLAGMNPASTGGINHTGSAPAALAALAGSQLRTANAANRAQQQQHEMTVSNDLIGCIIGKGGTKIAEIRQISGAMIRISNCEEREGGNTDRTITISGNPDSVALAQYLINMRISMETAGVPIPGYHYIAPSAIVKTPIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTZ0</accession>
    <name>A0AAU9FTZ0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Titin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_07040</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Myofibril</location>
            <location evidence="2">Sarcomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
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        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
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        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="CDD" id="cd11856">
        <property type="entry name" value="SH3_p47phox_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Muscle M-line assembly protein unc-89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000147">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000119">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000430">
        <property type="entry name" value="Sallimus, isoform P"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000962">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="twitchin isoform X4"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="44"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633:SF4">
        <property type="entry name" value="MYOPALLADIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="37"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="93"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="264"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="381"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="480"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="627"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="760"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="900"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
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            <authorList>
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</entry>
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            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF98780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="846" mass="91554" checksum="CCA0A8BEBD75F588" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTZ9</accession>
    <name>A0AAU9FTZ9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Group XIIA secretory phospholipase A2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_07377</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTZ9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010711">
        <property type="entry name" value="PLA2G12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12824">
        <property type="entry name" value="GROUP XII SECRETORY PHOSPHOLIPASE A2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12824:SF8">
        <property type="entry name" value="GXIVSPLA2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06951">
        <property type="entry name" value="PLA2G12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="198"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF99482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="228" mass="25426" checksum="56C3E8C9631C3BB8" modified="2024-11-27" version="1">MQFSWMKVAIYVLTFLTYAYSGYGSSTIVHLRDAIIAAEAIFGDVFKNLITVVRKFRTVHEVFDAAVEENCVFQCPPPDGGGPAPGAVQNKFYTPTADGCGSLGLRISTDYLPAVEMETCCNDHDICYDTCNSDKELCDLDFKRCLYKYCDSYEKSLTSDLMTKGCKAAAKMLFTGTLTLGCRSFLDSQKRSCYCAPSKASSYNGNKYANSKEKQQRQKSGWKDRNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUA5</accession>
    <name>A0AAU9FUA5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ganglioside-induced differentiation-associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_07475</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUA5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00570">
        <property type="entry name" value="GST_N_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000394">
        <property type="entry name" value="Gdap1, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44188">
        <property type="entry name" value="GDAP1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44188:SF1">
        <property type="entry name" value="GDAP1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="3">
        <location>
            <begin position="28"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="166"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="167"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF99617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="324" mass="37053" checksum="882E65E4947E2E1A" modified="2024-11-27" version="1">MGDQAKEIEAATPPAILQDFKAPVFADNKLVLYFHPYNFYSQKVLLVLYEKNIDFTPYVVDLCNGEQYSSWFLNLNPKGDVPVLQDGAFIIPNSTHIINYVESKFRGANHPVLKPDQSSAQYDQVMIYESAVGRVPVGALSLGSFIHDDLKLVPKAPFIGPVRQSCLKNNDKVLDLLRRSVDELESNKSALKQKLDIQIRRKDLVSCRDDFQRVLDAVRHVLLYVEQELSQQAPRNEWLTGNEVTLADISLGLLLHRLYQLGFENYYWSFGKLPQVEGYFLRFRQRQSYHKLQPSNFEILREMWAKTPGNYKLGAGAGFLVGHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUA6</accession>
    <name>A0AAU9FUA6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cuticle protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_07163</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUA6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051217">
        <property type="entry name" value="Insect_Cuticle_Struc_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236:SF79">
        <property type="entry name" value="CUTICULAR PROTEIN 50CB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236">
        <property type="entry name" value="STRUCTURAL CONTITUENT OF CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043526924" description="Cuticle protein 6" evidence="4">
        <location>
            <begin position="22"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="241"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="255"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF99204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="35748" checksum="0914E67712B4000B" modified="2024-11-27" version="1">MRSLSSLLVFTLFVLIIKVYATLGSPLAAKTEAYSKTAAANASKKAKVTNYFLHEPYGTNTYAFGYEIDDPQTQNVQFRDERRFVNGSVEGSYGYVRPDGLIEVTKYRADENGGFVAQTQSFQPGDEQAKAVWPTQRPGIIVDRHKVQSPANVTWDPKSHLNVSVSHVADDVAKQLKEQHGLDLNHIDVTKDVLQPAVLDVLQGKAQLKNQTRDGTPFQPVQNVIPNRFPIVPFMLPTDTEPFKATTEEPHPKGNGNSKYNRAKTNNAEKAPQVEAPESGLLPPPRPLANEAASDANWYQRIIDANRREFLNNLPNLSKVNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUA8</accession>
    <name>A0AAU9FUA8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_07496</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06608">
        <property type="entry name" value="STKc_myosinIII_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000003">
        <property type="entry name" value="TRAF2 and NCK-interacting protein kinase isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000006">
        <property type="entry name" value="TRAF2 and NCK-interacting protein kinase isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051700">
        <property type="entry name" value="STE20_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096:SF1">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="34"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="13">
        <location>
            <begin position="1276"/>
            <end position="1564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="317"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="447"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="732"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="875"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="948"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1021"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1142"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="495"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="510"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="521"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="552"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="562"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="620"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="741"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="773"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="820"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="876"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="948"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="958"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1021"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1034"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1061"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1094"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1104"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF99649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1590" mass="170339" checksum="FA56A22E169E0853" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUB0</accession>
    <name>A0AAU9FUB0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Titin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_07040</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Myofibril</location>
            <location evidence="2">Sarcomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="CDD" id="cd11856">
        <property type="entry name" value="SH3_p47phox_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000344">
        <property type="entry name" value="Muscle M-line assembly protein unc-89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000147">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000107">
        <property type="entry name" value="Myosin, light chain kinase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000119">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000430">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000966">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001019">
        <property type="entry name" value="Sallimus, isoform P"/>
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                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="18 19">RF1</strain>
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        <text evidence="14">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
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</entry>
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                <person name="Yamamoto D."/>
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        <source>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">RF1</strain>
        </source>
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</entry>
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</entry>
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                <person name="Yamamoto D."/>
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</entry>
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            <authorList>
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                <person name="Yamamoto D."/>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the perilipin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G5Z0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010890">
        <property type="term" value="P:positive regulation of triglyceride storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004279">
        <property type="entry name" value="Perilipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024:SF53">
        <property type="entry name" value="LIPID STORAGE DROPLETS SURFACE-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024">
        <property type="entry name" value="PERILIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03036">
        <property type="entry name" value="Perilipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFG02934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="339" mass="37795" checksum="9CAD4A7CD2B1D1DD" modified="2024-11-27" version="1">MASAEQKHATGSNGTAKNTNNKINSALTEQEQPKDAKDLLPHLESLERIIKLPVVNAAWDKSQDVYGKVKGKNRVFEWALTAAEDCVTRAVTTAAPFVTKLDRPISFVDQTLVKGIDKLEVKAPIIKDTPQEIYNQAKSKVIDVVQPHLERVVKFKAAGQQKAASLKDLAWQKANEVLATQYGSLAVNGVDTTTALAERLLEYYFPKSESDVEEDNDDKQNAVAHNGKSSENDIEVVGESCDYDREIDDRFLVAASTVFLSMSLKIMTEIVEYVRRNPEIINIIDRELNKFMCQFPPARIQSYTQSRPLADYPTRSLVASIEMSPDRSSRSKRATSTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5Z1</accession>
    <name>A0AAU9G5Z1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transient receptor potential cation channel trpm</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_02501</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1010">
        <property type="entry name" value="TRPM, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050927">
        <property type="entry name" value="TRPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057366">
        <property type="entry name" value="TRPM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041491">
        <property type="entry name" value="TRPM_SLOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032415">
        <property type="entry name" value="TRPM_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037162">
        <property type="entry name" value="TRPM_tetra_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800:SF1">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL TRPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL, SUBFAMILY M, MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18139">
        <property type="entry name" value="LSDAT_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25508">
        <property type="entry name" value="TRPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16519">
        <property type="entry name" value="TRPM_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="995"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1062"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1142"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="domain" description="TRPM SLOG" evidence="12">
        <location>
            <begin position="192"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="TRPM-like" evidence="13">
        <location>
            <begin position="517"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="10">
        <location>
            <begin position="936"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="domain" description="TRPM tetramerisation" evidence="11">
        <location>
            <begin position="1258"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="834"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1472"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1559"/>
            <end position="1585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1779"/>
            <end position="1804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1876"/>
            <end position="1909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1968"/>
            <end position="2009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2023"/>
            <end position="2042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1791"/>
            <end position="1802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1968"/>
            <end position="1983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1992"/>
            <end position="2009"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF18139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFG03186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2042" mass="227555" checksum="F5ECCBCA846D640A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5Z3</accession>
    <name>A0AAU9G5Z3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribonuclease 3</fullName>
            <ecNumber evidence="6">3.1.26.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Ribonuclease III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21 22">protein Drosha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">DMAD_02761</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Executes the initial step of microRNA (miRNA) processing in the nucleus, that is the cleavage of pri-miRNA to release pre-miRNA. Involved in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in fertility. Required for the function or synthesis of the let-7 miRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ribonuclease III family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.3" evidence="6"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070877">
        <property type="term" value="C:microprocessor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007506">
        <property type="term" value="P:gonadal mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031054">
        <property type="term" value="P:pre-miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031053">
        <property type="term" value="P:primary miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19877">
        <property type="entry name" value="DSRM_RNAse_III_meta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000002">
        <property type="entry name" value="Drosha ribonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000012">
        <property type="entry name" value="Drosha ribonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease III domain"/>
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        </source>
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    <sequence length="1300" mass="149162" checksum="3A51160D2F42D51F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
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                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Serpin_dom"/>
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        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
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        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
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        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
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        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF372">
        <property type="entry name" value="ACCESSORY GLAND PROTEIN ACP76A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="chain" id="PRO_5043728710" evidence="4">
        <location>
            <begin position="24"/>
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        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="5">
        <location>
            <begin position="28"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00022690"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00093"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFG03689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="367" mass="41787" checksum="871AF5512FDCC6F7" modified="2024-11-27" version="1">MNVLLNFAALLGVFLLLAVPIYSNHFASQLYSQASHANHHRNLVLSPATTNMALGLVFFGMRDCPELTQVLDTMRHGDWATDAIKMVNRLYVDRNIQLMPAYQEAISDDRMGQPAKKVDFSDPQVASEEINSFVRILTDNKIQRFMHRGHLGKDIQMAIANAAYFHGEWAGIKYSKPLPFYPLNGTEELLVDTLETYDALRYGSIDYLDAHVVELPYRSEQLSMIIILPRDPRQSLNKLSSLIRDVDLVPLTENLTKEFVHLQLPKFQVETRLPLETVLRPLGVKTMFHAPVLHKMIASRDRSHKIGTIMHYSAIEVSESGRGYSPLMMVSQQMGRKPIQFWANHPFIFAIKDSNHIYFMGHVVAPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6A7</accession>
    <name>A0AAU9G6A7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TRPL translocation defect protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_02544</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6A7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
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        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070300">
        <property type="term" value="F:phosphatidic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045494">
        <property type="term" value="P:photoreceptor cell maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.320.10:FF:000003">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038727">
        <property type="entry name" value="NadR/Ttd14_AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053227">
        <property type="entry name" value="TRPL-trafficking_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34932">
        <property type="entry name" value="TRPL TRANSLOCATION DEFECT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34932:SF1">
        <property type="entry name" value="TRPL TRANSLOCATION DEFECT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13521">
        <property type="entry name" value="AAA_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NadR/Ttd14 AAA" evidence="2">
        <location>
            <begin position="207"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG03241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="533" mass="59359" checksum="468C30BE87638A00" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6A8</accession>
    <name>A0AAU9G6A8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">LIM domain kinase 1</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_02400</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG03054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6A8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09365">
        <property type="entry name" value="LIM2_LIMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06754">
        <property type="entry name" value="PDZ_LIMK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000082">
        <property type="entry name" value="LIM domain kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000242">
        <property type="entry name" value="LIM domain kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000038">
        <property type="entry name" value="LIM domain kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000038">
        <property type="entry name" value="LIM domain kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000197">
        <property type="entry name" value="LIM domain kinase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050940">
        <property type="entry name" value="Actin_reg-Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485">
        <property type="entry name" value="LIM DOMAIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485:SF4">
        <property type="entry name" value="LIM DOMAIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 22" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 16" id="KW-0440">LIM domain</keyword>
    <keyword evidence="8 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="20">
        <location>
            <begin position="32"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="20">
        <location>
            <begin position="95"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="21">
        <location>
            <begin position="177"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="399"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="282"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="563"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1013"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1079"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1234"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1033"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1096"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1120"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="1238"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="1253"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040667"/>
        </source>
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                <person name="Llopart A."/>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFG04030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2259" mass="252673" checksum="8D7B742F56CE2A7D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6E9</accession>
    <name>A0AAU9G6E9_DROMD</name>
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        <submittedName>
            <fullName evidence="2">Serine-rich adhesin for platelets</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_02635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6E9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="entry name" value="FYVE_BSN_PCLO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="668"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="807"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="984"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="704"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="812"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="859"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="986"/>
            <end position="996"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG03351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1037" mass="112923" checksum="1B359D3FDF2CF22B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6F1</accession>
    <name>A0AAU9G6F1_DROMD</name>
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        <submittedName>
            <fullName evidence="5">Ran-binding proteins 9/10 homolog</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="ORF">DMAD_03113</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the RANBP9/10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
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        <property type="entry name" value="SPRY_RanBP9_10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ConA-like_dom_sf"/>
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    </dbReference>
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        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
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        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035782">
        <property type="entry name" value="SPRY_RanBP9/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        </location>
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            <end position="342"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="364"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFG04060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="632" mass="70176" checksum="634757E8EC637543" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6F2</accession>
    <name>A0AAU9G6F2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Troponin C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_02435</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the troponin C family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000177">
        <property type="entry name" value="Troponin C Ia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000103">
        <property type="entry name" value="Troponin C Ib"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
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        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
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        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="44"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="120"/>
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        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038202"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG03099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="154" mass="17283" checksum="08C48D7E7998D60B" modified="2024-11-27" version="1">MSDELTKEQIELLRNAFNAFDPEKNGYINTAMVGTILSMLGHQLDDATLADIIAEVDEDGSGQIEFEEFTTLAARFLVEEDAEAMMQELKEAFRLYDKEGNGYITTGVLREILRELDDKLTNDDLDMMIEEIDSDGSGTVDFDEFMEVMTGGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6F6</accession>
    <name>A0AAU9G6F6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Potassium voltage-gated channel subfamily KQT member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_03088</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
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        <reaction evidence="12">
            <text>K(+)(in) = K(+)(out)</text>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="entry name" value="Voltage-gated potassium channels"/>
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    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="40"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="239"/>
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    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="39"/>
            <end position="270"/>
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    </feature>
    <feature type="domain" description="Potassium channel voltage dependent KCNQ C-terminal" evidence="16">
        <location>
            <begin position="423"/>
            <end position="531"/>
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        <location>
            <begin position="339"/>
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    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
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        <location>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF03520"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG04007.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="808" mass="90638" checksum="376094478213E906" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6F9</accession>
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        <submittedName>
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        </submittedName>
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        <name evidence="14" type="ORF">DMAD_02477</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="14 15">RF1</strain>
        </source>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.</text>
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        <property type="entry name" value="SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member"/>
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        <property type="entry name" value="SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member"/>
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        <property type="entry name" value="SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member"/>
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        <property type="entry name" value="Helicase_C-like"/>
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        <property type="entry name" value="SANT/Myb"/>
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        <property type="entry name" value="SLIDE"/>
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        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
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        <property type="entry name" value="SNF2_N"/>
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        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF49">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HAND"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF09111">
        <property type="entry name" value="SLIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Homeodomain-like"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="148"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="443"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="13">
        <location>
            <begin position="803"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="60"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="979"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFG03155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1034" mass="120059" checksum="EDB3EBCFE641821C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GGA0</accession>
    <name>A0AAU9GGA0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14114">
        <property type="entry name" value="STKc_Twitchin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform C"/>
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    </dbReference>
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        <property type="entry name" value="Bent, isoform C"/>
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        <property type="entry name" value="Myosin light chain kinase"/>
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        <property type="entry name" value="Myosin-binding protein C, slow type"/>
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        <property type="entry name" value="Protein CBR-UNC-22"/>
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        <property type="entry name" value="twitchin isoform X2"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform I"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
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        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF151">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="33"/>
    </dbReference>
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        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="37"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="9"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="119"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="228"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="335"/>
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        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="438"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="543"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="640"/>
            <end position="728"/>
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    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="746"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="1680"/>
            <end position="1768"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="1775"/>
            <end position="1859"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="2053"/>
            <end position="2150"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="2153"/>
            <end position="2251"/>
        </location>
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                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GGA4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20035">
        <property type="entry name" value="FH_FOXQ2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000352">
        <property type="entry name" value="Forkhead box Q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047519">
        <property type="entry name" value="FH_FOXQ2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF343">
        <property type="entry name" value="FORK-HEAD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fork-head" evidence="6">
        <location>
            <begin position="127"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="4">
        <location>
            <begin position="127"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="217"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="413"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="487"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="239"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="362"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="413"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="487"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="510"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG06684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="588" mass="65052" checksum="E1604E5EA8F9EF18" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GGB4</accession>
    <name>A0AAU9GGB4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06699.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome dot"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14114">
        <property type="entry name" value="STKc_Twitchin_like"/>
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    </dbReference>
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        <property type="entry name" value="Bent, isoform C"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000440">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000097">
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000642">
        <property type="entry name" value="Bent, isoform F"/>
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    </dbReference>
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        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform H"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001892">
        <property type="entry name" value="Bent, isoform H"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000460">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000504">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000505">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000147">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002083">
        <property type="entry name" value="Protein CBR-UNC-22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000160">
        <property type="entry name" value="Titin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000003">
        <property type="entry name" value="Titin isoform E"/>
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    </dbReference>
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        <property type="entry name" value="titin isoform X1"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:000127">
        <property type="entry name" value="titin isoform X1"/>
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    </dbReference>
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        <property type="entry name" value="titin isoform X1"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000249">
        <property type="entry name" value="twitchin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000056">
        <property type="entry name" value="twitchin isoform X4"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000936">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000006">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000775">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001164">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002180">
        <property type="entry name" value="Uncharacterized protein, isoform G"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000876">
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001033">
        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000935">
        <property type="entry name" value="Uncharacterized protein, isoform I"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001001">
        <property type="entry name" value="Uncharacterized protein, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000051">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000553">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000559">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000748">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001016">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="76"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF151">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
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<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="16 17">RF1</strain>
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        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="5"/>
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        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="8"/>
    </dbReference>
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        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="21" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="983"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like calcium-binding" evidence="19">
        <location>
            <begin position="523"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="526"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like calcium-binding" evidence="19">
        <location>
            <begin position="564"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="567"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="16">
        <location>
            <begin position="650"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="16">
        <location>
            <begin position="694"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="16">
        <location>
            <begin position="737"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="16">
        <location>
            <begin position="781"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="876"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="928"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="936"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="948"/>
            <end position="961"/>
        </location>
    </feature>
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        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
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    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="295"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="314"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
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    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="333"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="354"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="361"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="442"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="449"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="461"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="493"/>
            <end position="511"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
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        <source>
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        </source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00179"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF88817.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1061" mass="117698" checksum="BE33CAF98C76A9B3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ERE9</accession>
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            <fullName evidence="6">carboxylesterase</fullName>
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        <name evidence="8" type="ORF">DMAD_08271</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00039155"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="573" mass="65492" checksum="B78C4EA9C9C0AC5F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ERG9</accession>
    <name>A0AAU9ERG9_DROMD</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">DMAD_08285</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF89548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="167" mass="19416" checksum="7AE5A17112A233BF" modified="2024-11-27" version="1">MAAEPFMIGDGPHLREDPWSNVPPQDYRFFYEALNFELEETLAHRNSGSGDAEQSPREINRARIDPLIFYDDIEGQQSELDDHEFEDSEEDDGPLNMGYLSLGPHFLEAQPHFRPHHHVPRRPLLSPRYTLSIEFDGSTCKQIFKTLALMAWQTALRLVYTELYFMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZP2</accession>
    <name>A0AAU9EZP2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_08635</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EZP2"/>
    <dbReference type="SMR" id="A0AAU9EZP2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23790">
        <property type="entry name" value="UBCc_UBE2A_2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000004">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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                <person name="Yamamoto D."/>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="163"/>
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        <location>
            <begin position="199"/>
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        </location>
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        <location>
            <begin position="219"/>
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        <location>
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        <location>
            <begin position="449"/>
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        <location>
            <begin position="474"/>
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        <location>
            <begin position="572"/>
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        <location>
            <begin position="605"/>
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        <location>
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        <location>
            <begin position="748"/>
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        <location>
            <begin position="769"/>
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        <location>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF90134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1211" mass="129871" checksum="C5497C71C2808F88" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZV7</accession>
    <name>A0AAU9EZV7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_09708</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
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    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
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        <location>
            <begin position="25"/>
            <end position="149"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF91425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="149" mass="16574" checksum="2F6EE7324DDC28BC" modified="2024-11-27" version="1">MSTSNSLSIARLGLFLSILGLVAAQLKRESGPCQANGEYCQMHEDCCSEKCMTYLNVCARKIDEGLPAHMSHHSNSIYDTDVKESADKFPDIVEIQTLPNESEQTPQERQGMKCNPVGGPCNVSEECCSKICHFYAHKCVTKKNVEQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F3A7</accession>
    <name>A0AAU9F3A7_DROMD</name>
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        <submittedName>
            <fullName evidence="17">Dynein heavy chain 5 axonemal</fullName>
        </submittedName>
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        <name evidence="17" type="ORF">DMAD_07853</name>
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    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
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        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="17 18">RF1</strain>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
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        <text evidence="2">Belongs to the dynein heavy chain family.</text>
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        <property type="term" value="P:microtubule-based movement"/>
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        <property type="entry name" value="Region D6 of dynein motor"/>
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    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="AAA_6"/>
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        <property type="entry name" value="AAA_9"/>
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        <property type="entry name" value="AAA_lid_11"/>
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        <property type="entry name" value="AAA_lid_11_sf"/>
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        <property type="entry name" value="DHC"/>
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        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056759">
        <property type="entry name" value="DYH2-5-8_CC"/>
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        <property type="entry name" value="Dynein_2_N"/>
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        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
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        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
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        <property type="entry name" value="Dynein_C"/>
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        <property type="entry name" value="Dynein_HC_stalk"/>
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        <property type="entry name" value="Dynein_heavy_tail"/>
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        <property type="entry name" value="Dynein_linker_3"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532">
        <property type="entry name" value="MALE FERTILITY FACTOR KL5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA_8"/>
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        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25007">
        <property type="entry name" value="DYH2-5-8_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
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    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="2037"/>
            <end position="2184"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2319"/>
            <end position="2448"/>
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    <feature type="domain" description="AAA+ ATPase" evidence="16">
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            <begin position="2672"/>
            <end position="2845"/>
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            <begin position="16"/>
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    <feature type="region of interest" description="Disordered" evidence="15">
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            <begin position="110"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="951"/>
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            <begin position="3298"/>
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        <location>
            <begin position="3518"/>
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        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="21"/>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="122"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="979"/>
            <end position="994"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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        <source>
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        <source>
            <dbReference type="SMART" id="SM00382"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    <sequence length="4539" mass="519655" checksum="303CF16FC14E5243" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F3D1</accession>
    <name>A0AAU9F3D1_DROMD</name>
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        <recommendedName>
            <fullName evidence="3">BAH domain-containing protein</fullName>
        </recommendedName>
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    <gene>
        <name evidence="4" type="ORF">DMAD_09729</name>
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    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
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        <property type="entry name" value="TNRC18_BAHCC1-like_SH3"/>
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        <property type="entry name" value="PROTEIN WINGED EYE"/>
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        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1515"/>
            <end position="1524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF91446.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1786" mass="191761" checksum="02265FA50F3D4B60" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F3E0</accession>
    <name>A0AAU9F3E0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mannosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_09294</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the eighth mannose residue in an alpha-1,6 linkage onto Man(7)GlcNAc(2)-PP-dolichol to produce Man(8)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29535"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19518"/>
            <dbReference type="Rhea" id="RHEA-COMP:19519"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132517"/>
            <dbReference type="ChEBI" id="CHEBI:132519"/>
            <dbReference type="EC" id="2.4.1.260"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:29536"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F3E0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052917">
        <property type="term" value="F:dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF1">
        <property type="entry name" value="DOL-P-MAN:MAN(7)GLCNAC(2)-PP-DOL ALPHA-1,6-MANNOSYLTRANSFERASE"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="589"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="151"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF89090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="613" mass="67150" checksum="78B8771CE3FF1134" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F3F8</accession>
    <name>A0AAU9F3F8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Protein mini spindles</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07932</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TOG/XMAP215 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061863">
        <property type="term" value="F:microtubule plus end polymerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000052">
        <property type="entry name" value="Cytoskeleton associated protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000019">
        <property type="entry name" value="Cytoskeleton-associated protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000050">
        <property type="entry name" value="Cytoskeleton-associated protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000410">
        <property type="entry name" value="Mini spindles, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000063">
        <property type="entry name" value="Putative cytoskeleton-associated protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
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    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045110">
        <property type="entry name" value="XMAP215"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12609">
        <property type="entry name" value="MICROTUBULE ASSOCIATED PROTEIN XMAP215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="12">
        <location>
            <begin position="268"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="10">
        <location>
            <begin position="440"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="12">
        <location>
            <begin position="576"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="12">
        <location>
            <begin position="848"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="12">
        <location>
            <begin position="1181"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="225"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="507"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="799"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1102"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1410"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1453"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1481"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1568"/>
            <end position="1590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="225"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="251"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="550"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="799"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="828"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1105"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1481"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1568"/>
            <end position="1578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF89105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2081" mass="229078" checksum="37D39F5B92897A40" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F3F9</accession>
    <name>A0AAU9F3F9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="24">Glutamate receptor ionotropic kainate 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_09319</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF90889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06382">
        <property type="entry name" value="PBP1_iGluR_Kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13714">
        <property type="entry name" value="PBP2_iGluR_Kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000105">
        <property type="entry name" value="Eye-enriched kainate receptor, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000061">
        <property type="entry name" value="Glutamate receptor, ionotropic kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000147">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10 24" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="21" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043650431" evidence="21">
        <location>
            <begin position="33"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="545"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="622"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="810"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="22">
        <location>
            <begin position="414"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="23">
        <location>
            <begin position="424"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="866"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="871"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="500"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="505"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="672"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="673"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="720"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="17">
        <location>
            <position position="650"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="17">
        <location>
            <position position="678"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="732"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="BFF90889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="909" mass="101564" checksum="CDAB1625DFF87E9F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F3G3</accession>
    <name>A0AAU9F3G3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dual-specificity kinase</fullName>
            <ecNumber evidence="2">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_09329</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
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            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
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            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF90904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PKc_CLK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dual specificity protein kinase CLK2"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.200.20:FF:000061">
        <property type="entry name" value="Dual specificity protein kinase CLK2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CLK_kinases"/>
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    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="930"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
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    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="16">
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    <feature type="region of interest" description="Disordered" evidence="16">
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    <feature type="region of interest" description="Disordered" evidence="16">
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    <feature type="region of interest" description="Disordered" evidence="16">
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    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="748"/>
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    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1257"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="32"/>
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        <location>
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        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
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        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="510"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="640"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="823"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="839"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1264"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1275"/>
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    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="959"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFF90904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1284" mass="143265" checksum="27718C66F01D74A1" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDA0</accession>
    <name>A0AAU9FDA0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lysosomal aspartic protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_11457</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDA0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF93643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="122" mass="12976" checksum="91B38B9A4C478570" modified="2024-11-27" version="1">MASASSDGNSLCSDCQAIADTGTSLIVAPYEAYEILVDILSVDEDGYVACSSVSSLPDVTFNIGGTDFTLTPASYIIQSDGNCMSAFEYMGTDFWILGDVFIGQYYTEFDLGNNRIGFAPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDA4</accession>
    <name>A0AAU9FDA4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_11444</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 24">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="CDD" id="cd05739">
        <property type="entry name" value="IgI_3_RPTP_IIa_LAR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14554">
        <property type="entry name" value="R-PTP-LAR-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14553">
        <property type="entry name" value="R-PTPc-LAR-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000010">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000002">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000001">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase F isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001111">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001197">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001219">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001274">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001000">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001102">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001130">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001015">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001564">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001191">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF6">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
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            <taxon>Pterygota</taxon>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
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            <name>Mg(2+)</name>
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        <subcellularLocation>
            <location evidence="1 15">Membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
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        <text evidence="2 15">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Phospholipid-transporting ATPase"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
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        <property type="entry name" value="ATPase_P-typ_P_site"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
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        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF218">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="492"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="537"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1252"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1284"/>
            <end position="1304"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
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</entry>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
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        <text evidence="1 6">Belongs to the aldehyde dehydrogenase family.</text>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDC1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="ALDH_F1AB_F2_RALDH1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
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            <begin position="502"/>
            <end position="521"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="605" mass="66855" checksum="5782268FA6EA3FD3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDG0</accession>
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    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        </subcellularLocation>
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        <location>
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        </location>
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        </location>
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        </location>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="510" mass="54811" checksum="2DD18994F7ED1922" modified="2024-11-27" version="1">MLLPSWHLIGALLVLTATAGDSTNNKIILPQILNGGDAPGKHPSGTKTASGATNGVGGGVGHVGVIGIATGTGNTVVGSNGVIVAGGGVVANGQTTNLNTVVEEPEFTEYIENVTVPAGRNVKLGCSVKNLGSYKVAWMHFEQSAILTVHNHVITRNPRISVTHDKHDRHRTWYLHINNVHEEDKGRYMCQINTVTAKTQFGYLNVVVPPNIDDSLSSSDVIVREGANISLRCRASGSPRPIIKWKRDDNSRIAINKNHIVNEWEGDTLEITRISRLDMGAYLCIASNGVPPTVSKRIKVSVDFPPMLLIPHQLVGAPEGFNVTIECFTEAHPTSLNYWTRGEGPIIHDSHKYKVESSVGVPAYKTHMKLTIINVSSGDDGIYKCVAKNPRGETDGIIRLYVSYPPTTVSSGTTDSHWERENGINGNNYAYGGSDGQRSIYGQDKNKTQNPLAANGNGNTQDSEAAGANRGAQPAKAIAWMLLFLPATTILLTIRQPAIGSHSSTVSVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDG2</accession>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="5" type="ORF">DMAD_11523</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DUF4793 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE APD1-4 MIDDLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16041">
        <property type="entry name" value="APD1-4_M"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
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        <location>
            <begin position="80"/>
            <end position="148"/>
        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="491" mass="55790" checksum="58C38014C0A133A8" modified="2024-11-27" version="1">MQPLTEYRFEETRKMHGVKRVLIFCLLIVILPATLIIMPLHLRKTVFADVVYPMAESDIIEIRAGISSIFCSKHTLKMTSSFNAFQLRNKPEISTNRKHIRLKKSMTLPDDTLEYWGFFLLKGANVRVKFCSRYDGSRILIVKGQRQLNLCGLTDHNKNKLGANYAKGHEQVKVFFEDKMEMEGPSAPAPEGGVLEHENHGGEDLSEEPTPQQQPLHLPTGLTLAGPSTATTSHKPIRKKLKKGTPHLPIPHHLVHSETPGTPQQHELTADAVQHSSGDRVVAPTPSVAQQKRQRKRELYERLYNRSKRENVYDRKYSHGGNAVNFTETDESNSVSSFENGLFLCFNGAILLSDFFKPNANCTDPHFMERAPNNSAQALQNVIEDGYYYYIFYSDNDEVRNDIHVIFDIYKPTFQYSNLSESQSCLNTTECSFNISFLSDEIVVVEVPTRDGIEHEEDDISYLVSTCHPRSEIYALFPITVMILILCCSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDG8</accession>
    <name>A0AAU9FDG8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ficolin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_11539</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
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    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF327">
        <property type="entry name" value="FI21813P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF93753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="137" mass="15461" checksum="C13F073654493338" modified="2024-11-27" version="1">MMTAAEPQELYIDLGDVNGTYRHAKYDNFIVGSEEESFVLKSLGNYSGNAGDSLREHEKAKFATDDRDSPNKCVHSYDGPWWFNGSSCGKSSLNGKYFKEGIADGRNGVSWGHWNMFDYKLSLTFVKMTIRPRSPQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDH1</accession>
    <name>A0AAU9FDH1_DROMD</name>
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        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">DMAD_11498</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BFF93696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
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        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037370">
        <property type="entry name" value="Pleckstrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12092:SF16">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12092">
        <property type="entry name" value="PLECKSTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="163"/>
            <end position="267"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="464"/>
            <end position="548"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
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            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
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        <location>
            <begin position="109"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="501"/>
        </location>
    </feature>
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            <begin position="523"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="605"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="640"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="743" mass="81428" checksum="F95F4D0572958B0D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDH2</accession>
    <name>A0AAU9FDH2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">DMAD_11532</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BFF93743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <location>
            <begin position="40"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="349"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="562"/>
            <end position="600"/>
        </location>
    </feature>
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        <location>
            <begin position="157"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="73"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="349"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF93743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="690" mass="79338" checksum="89C262C2D7F24D20" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDH9</accession>
    <name>A0AAU9FDH9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ethanolamine-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CTP:phosphoethanolamine cytidylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_11596</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.14" evidence="10"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDH9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004306">
        <property type="term" value="F:ethanolamine-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ECT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CCT"/>
    </dbReference>
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        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044608">
        <property type="entry name" value="Ect1/PCYT2"/>
    </dbReference>
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        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780:SF2">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="13">
        <location>
            <begin position="40"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Cytidyltransferase-like" evidence="13">
        <location>
            <begin position="233"/>
            <end position="326"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF93821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="397" mass="44236" checksum="9235510D4B407856" modified="2024-11-27" version="1">MSDKQVNGCSSAKSYSSPCNGNTTQKQEESPINGKDVRVWCDGCYDMVHFGHANSLRQAKALGDKVIVGIHTDAEITKHKGPPVFTEEERVKMVKGIKWVDEVVLGAPYVTTLEVLDQNNCDFCVHGDDITMTAEGVDTYHLVKSANRYKEVRRTAGVSTTDLVGRMLLLTRNHFRQGSAEYDIEKEGSSNMGQDSAAKSPWTGCSQFLPTTQKIIQFSDGKSPNAGDKIVYVAGAFDLFHVGHLDFLEKASKLGDYLIVGLHTDPVVNSYKGSNYPIMNLHERVLSVLACKFVNEVVIGAPYCVTEELLEHFKIDVVCHGRTPIAMEDGKIDPYAVPKTKAIFELIDSGNEMTTERIVERIISHRLEYERRNQAKEKKEIEAFEALQRQKQTQKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDI3</accession>
    <name>A0AAU9FDI3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DMAD_11522</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9FDI3"/>
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        <property type="component" value="Chromosome U"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004245">
        <property type="entry name" value="DUF229"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974:SF9">
        <property type="entry name" value="DUF229 DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974">
        <property type="entry name" value="FI08016P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02995">
        <property type="entry name" value="DUF229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF93727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="666" mass="77575" checksum="BA6331E0D64112ED" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FDI4</accession>
    <name>A0AAU9FDI4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Inhibitor of growth protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_11554</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ING family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FDI4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16859">
        <property type="entry name" value="ING_ING4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15586">
        <property type="entry name" value="PHD_ING4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000016">
        <property type="entry name" value="Inhibitor of growth protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
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        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
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        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF42">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="222"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="116"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="165"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF93768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="275" mass="30633" checksum="A086FEF217AE1093" modified="2024-11-27" version="1">MSSAMYLENYLDGLESLPTELERNFKLMRKLDDRAQTAMKSIDSHAKDFMRKLAESGPLCEEERKERLEDIKALFGKAKEYSDDKVQLAIQTYELVDKQIRRLDNDLARFEGEIQEKASSTRAKSEEAVAKKGRKKAKDSKVTGKKKKSASSDEETGRGGGGGTQTIANVSLNSSSNAGGQGTKKKKSKKVVEADDSEKDSCHTAATHPSDVMDMPVDPNEPTYCLCHQVSYGEMIGCDNPDCPIEWFHFACVGLTTKPKGKWFCPKCTQDRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLR3</accession>
    <name>A0AAU9FLR3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulatory protein AlgP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_05363</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLR3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043739839" evidence="2">
        <location>
            <begin position="22"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="257"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF96812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="331" mass="34693" checksum="D73B4D795EA40A05" modified="2024-11-27" version="1">MKISAALALLVIAGFVASGDALPARKRMVYYQQPAAAEWYGSAPQQRIMYMQYVQPGRTHARSTQAASALVAGETVATGTYLKESVDELSAEAQVEDVLAAAGAHGATSVAEAYPEENPVVQVAIDADVVPEAKPETEPEAGAAVVETDESAKVPREFNFVSEEAAAAAVAAEEEAIAEPVVPANRYLPAKKKVIVELEQSEEDEPEVAAIEDEEAENTVSDDVDEDEEELLVVKPVIKNPARVPNARRPAAKKPVKAATPAAKPSKKPAAPLPAGTFFPIDFGGTNGGAIAIANSFSTGEGGAATSHAIAYGSPDSVRARVRPNPSKFRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLR7</accession>
    <name>A0AAU9FLR7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fucosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_05117</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus</location>
            <location evidence="1 12">Golgi stack membrane</location>
            <topology evidence="1 12">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
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            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF96733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2034" mass="222540" checksum="61C36AD2FAD9A467" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLS2</accession>
    <name>A0AAU9FLS2_DROMD</name>
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        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 1</fullName>
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        </alternativeName>
    </protein>
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        <name evidence="12" type="ORF">DMAD_05216</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
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        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF96622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome J"/>
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        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:transcription coregulator activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051999">
        <property type="entry name" value="Mediator_complex_subunit_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019680">
        <property type="entry name" value="Mediator_Med1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12881">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12881:SF10">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10744">
        <property type="entry name" value="Med1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1427"/>
            <end position="1508"/>
        </location>
    </feature>
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        <location>
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            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="829"/>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="878"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="930"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="963"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
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        </location>
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        </location>
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    </feature>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031254"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU364059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF96622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1508" mass="153318" checksum="32252815950FB534" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLS4</accession>
    <name>A0AAU9FLS4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA exonuclease 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_05205</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLS4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Interferon-stimulated exonuclease gene 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037431">
        <property type="entry name" value="REX4_DEDDh_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF158">
        <property type="entry name" value="RNA EXONUCLEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="9">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF96609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="262" mass="29443" checksum="D966654F1342528E" modified="2024-11-27" version="1">MSSSEPDQMRLGNKPKTSRTSSNSREKTMQQPNVYRRAAGSNWLAATVSSSDNKVHVAKAAEDGTAPDSEHLTKSARTRQRKMAQRNRYLAMDCEMVGVGHNGQEDMLARVSIVNIAGHVLLDKHVKPRHPVTDYRTSVSGIRPHDIENAEDFATVQNEVVKMIHGKILVGHALRNDLAVLNIKHPFEQIRDTSRYKPLCKLVSNGHTPSLKRLTMAVLGQEIQTGEHNSVEDARSAMGIYNRIAADWEKYLDKKQLQQQRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLV1</accession>
    <name>A0AAU9FLV1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Glypican-6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_05173</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Cell surface proteoglycan.</text>
    </comment>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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            <taxon>Insecta</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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            <begin position="535"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="552"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="582"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF96941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="618" mass="62282" checksum="21DB41C9643987B9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLW7</accession>
    <name>A0AAU9FLW7_DROMD</name>
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        <submittedName>
            <fullName evidence="5">Fat-body protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_05199</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLW7"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:energy homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Hemocyanin, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1370.10">
        <property type="entry name" value="Hemocyanin, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hemocyanin/hexamerin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005203">
        <property type="entry name" value="Hemocyanin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037020">
        <property type="entry name" value="Hemocyanin_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005204">
        <property type="entry name" value="Hemocyanin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036697">
        <property type="entry name" value="Hemocyanin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511:SF5">
        <property type="entry name" value="FAT-BODY PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511">
        <property type="entry name" value="LARVAL STORAGE PROTEIN/PHENOLOXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03723">
        <property type="entry name" value="Hemocyanin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hemocyanin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48050">
        <property type="entry name" value="Hemocyanin, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043392541" evidence="2">
        <location>
            <begin position="24"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="Hemocyanin N-terminal" evidence="3">
        <location>
            <begin position="43"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Hemocyanin C-terminal" evidence="4">
        <location>
            <begin position="429"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="524"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF96597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="595" mass="68167" checksum="95546020AD5B4C7E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAU9FLX6_DROMD</name>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_05193</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Recycling endosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLX6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Nuclear fallout, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FIP-RBD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019018">
        <property type="entry name" value="Rab-bd_FIP-RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057316">
        <property type="entry name" value="Rab11-FIP3/4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051977">
        <property type="entry name" value="Rab11-interacting_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15726:SF7">
        <property type="entry name" value="NUCLEAR FALLOUT, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15726">
        <property type="entry name" value="RAB11-FAMILY INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25450">
        <property type="entry name" value="Rab11-FIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09457">
        <property type="entry name" value="RBD-FIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144270">
        <property type="entry name" value="Eferin C-derminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51511">
        <property type="entry name" value="FIP_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FIP-RBD" evidence="10">
        <location>
            <begin position="443"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="27"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="222"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="78"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="166"/>
            <end position="179"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004654"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF96588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="510" mass="57942" checksum="DEC34EFF441DF3DF" modified="2024-11-27" version="1">MAPMPRIQLPNSNASIKANTNSFLYAETMSGDSSPTPSSPPSSTAGVAKSQCSSLSDGESFEGYGENEFPAQLREGRSGSSPNNNSISNNNNNNIIGNKGNNNLSVGSSNSHHNHSGNSNDGNNNLNASVGIELDLAPHVGSSTPQDDDELNIMPRDNWARRSLRRTPTSTGRRQISTNALASQLYRSSSFNSSGRSSNCDTTEDMYSDISLENRHDYDYRLELLQRKVDDLSDTQNIAEDRTTRTKTEYAVLQARYHMLEEQFRESELRSEERLAEEQKRHREILARVEREASLQNENCQMRIRATEIEANALRDEAQRLRVQCDKQANDLHRTEEQLELARDQIAALQQEYEEQQQTLRKQEIEKKSTEELMLELSRELQRARDENGARAMPTTSPESIRLEELHQELEEMRQKNRSLEEQNEELQATMLTNQASMLNSGVEQGRHLLNGTLGSLAQELEEMSQAQLQQAFQEKEDENVRLKHYIDTILLNIVENYPQLLEVKPMERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLY0</accession>
    <name>A0AAU9FLY0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock 70 kDa protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_05207</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLY0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10228">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000171">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000004">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000024">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
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        <property type="entry name" value="Hsp_70_fam"/>
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        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
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        <property type="entry name" value="HSP70"/>
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        <property type="entry name" value="HEATSHOCK70"/>
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        <property type="entry name" value="HSP70_3"/>
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            <dbReference type="ARBA" id="ARBA00007381"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
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        <source>
            <dbReference type="EMBL" id="BFF96612.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="807" mass="88866" checksum="48156E424A4BB6CA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLY1</accession>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9 10">RF1</strain>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR44981:SF2">
        <property type="entry name" value="PERICENTRIN-LIKE PROTEIN, ISOFORM F"/>
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        <property type="entry name" value="PACT_coil_coil"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2759"/>
            <end position="2828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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    <feature type="region of interest" description="Disordered" evidence="7">
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    <feature type="region of interest" description="Disordered" evidence="7">
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        </location>
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        <location>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF10495"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF96501.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="11">Malate dehydrogenase</fullName>
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        </recommendedName>
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        <name evidence="14" type="ORF">DMAD_05257</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="6 11">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
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    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0AAU9FLY6"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0520">NAD</keyword>
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    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="12">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="151"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="12"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF96669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="321" mass="34159" checksum="673A32DD622EC96D" modified="2024-11-27" version="1">MARTAFKVSVVGAGGGIGQPLSLLLRKVKGIDELSLHDLRGTKGVAADTGHISEHGTVSGYVGEDELEPALLGANLVVVTAGMPRVPGMTRDQLMQANGGIAIHVIRAMACACPEALIAITTNPINIIVPTAAEVLKEAGVFDPHRLFGITTLDVVRSRKFIGDFMNINPRKVNIPVIGGHTGPTILPLFSQCQPEFRGDTEDILRLTNRIKEAGTEVVMAKGGAGSATLSMAYATCCFVKSLIRAQNGQEGIIECAFVASSVADALFFAGPVELGKQGVKRCLKLPVLNEFEQKSLDNLLPILRKNIEAGIQFAKTFKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FM30</accession>
    <name>A0AAU9FM30_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Tenascin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_05398</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Synapse</location>
            <location evidence="17">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tenascin family. Teneurin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099559">
        <property type="term" value="P:maintenance of alignment of postsynaptic density and presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048790">
        <property type="term" value="P:maintenance of presynaptic active zone structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034116">
        <property type="term" value="P:positive regulation of heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040017">
        <property type="term" value="P:positive regulation of locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001941">
        <property type="term" value="P:postsynaptic membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097090">
        <property type="term" value="P:presynaptic membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034110">
        <property type="term" value="P:regulation of homotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000331">
        <property type="term" value="P:regulation of terminal button organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051124">
        <property type="term" value="P:synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016200">
        <property type="term" value="P:synaptic target attraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048499">
        <property type="term" value="P:synaptic vesicle membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.180.10.10:FF:000008">
        <property type="entry name" value="Odz, odd Oz/ten-m homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000021">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000474">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000013">
        <property type="entry name" value="Teneurin transmembrane protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000033">
        <property type="entry name" value="teneurin-a isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000560">
        <property type="entry name" value="Teneurin-m-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000189">
        <property type="entry name" value="Teneurin-m-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.180.10.10">
        <property type="entry name" value="RHS repeat-associated core"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057627">
        <property type="entry name" value="FN-plug_TEN1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056823">
        <property type="entry name" value="TEN-like_YD-shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056822">
        <property type="entry name" value="TEN_NHL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056820">
        <property type="entry name" value="TEN_TTR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051216">
        <property type="entry name" value="Teneurin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057629">
        <property type="entry name" value="Teneurin1-4_GBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028916">
        <property type="entry name" value="Tox-GHH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006530">
        <property type="entry name" value="YD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01643">
        <property type="entry name" value="YD_repeat_2x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219">
        <property type="entry name" value="TENEURIN AND N-ACETYLGLUCOSAMINE-1-PHOSPHODIESTER ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219:SF72">
        <property type="entry name" value="TENEURIN-M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25024">
        <property type="entry name" value="EGF_TEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24329">
        <property type="entry name" value="FN-plug_TEN1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23093">
        <property type="entry name" value="GBD_Tenm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25021">
        <property type="entry name" value="TEN_NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25023">
        <property type="entry name" value="TEN_YD-shell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23538">
        <property type="entry name" value="Teneurin_ABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15636">
        <property type="entry name" value="Tox-GHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25020">
        <property type="entry name" value="TTR_TEN1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 19" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <keyword evidence="5" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="1160"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="1198"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="1362"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="276"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="346"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="493"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="589"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="666"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="769"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="868"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="907"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="3316"/>
            <end position="3355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="276"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="297"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FM46</accession>
    <name>A0AAU9FM46_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Guanylate cyclase</fullName>
            <ecNumber evidence="3 15">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_05405</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="3 15"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FM46"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001653">
        <property type="term" value="F:peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06370">
        <property type="entry name" value="PBP1_SAP_GC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14042">
        <property type="entry name" value="PK_GC-A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000420">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000004">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000265">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000311">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011645">
        <property type="entry name" value="HNOB_dom_associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07701">
        <property type="entry name" value="HNOBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043986825" description="Guanylate cyclase" evidence="17">
        <location>
            <begin position="35"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="515"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="19">
        <location>
            <begin position="903"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="533"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1207"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="540"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1207"/>
            <end position="1226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF96868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1233" mass="137992" checksum="05BF8C6C1E5A6759" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FM71</accession>
    <name>A0AAU9FM71_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Collagen alpha-1(XV) chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_05327</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FM71"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005594">
        <property type="term" value="C:collagen type IX trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00247">
        <property type="entry name" value="Endostatin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000137">
        <property type="entry name" value="Multiplexin collagen isoform Ap3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000048">
        <property type="entry name" value="Multiplexin collagen isoform Ap3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1620.70:FF:000001">
        <property type="entry name" value="Multiplexin collagen isoform Ap3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010515">
        <property type="entry name" value="Collagenase_NC10/endostatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045463">
        <property type="entry name" value="XV/XVIII_trimerization_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1116">
        <property type="entry name" value="MACROPHAGE RECEPTOR WITH COLLAGENOUS STRUCTURE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20010">
        <property type="entry name" value="Collagen_trimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06482">
        <property type="entry name" value="Endostatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0176">Collagen</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043538165" evidence="4">
        <location>
            <begin position="21"/>
            <end position="1016"/>
        </location>
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            <begin position="32"/>
            <end position="222"/>
        </location>
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            <end position="337"/>
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            <end position="683"/>
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        </location>
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            <end position="426"/>
        </location>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1016" mass="107077" checksum="CE7129156E079A37" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAU9FM72_DROMD</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
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            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
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        </subcellularLocation>
    </comment>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        </source>
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    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="225" mass="24527" checksum="CC6559EE088E09B9" modified="2024-11-27" version="1">MCHQMAMKKEAQEKLKAEELRKAQAASEIPIIWILGGPGCGKGTQCAKIVEKYGFTHLSSGDLLRNEVASGSDKGRQLQAVMSSGGLVSNDEVLSLLNDAISRAKGSSKGFLIDGYPREKNQGVAFEERIAPADLALYFDCSEDTMLKRIMARAAAAAVKRADDNEQTIRARLQTFKLNTSAILELYADKTLTINAERDVDDIFQEVVQAIECVLKKKAKAAAQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FM79</accession>
    <name>A0AAU9FM79_DROMD</name>
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        <submittedName>
            <fullName evidence="4">Synaptotagmin-3</fullName>
        </submittedName>
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        <name evidence="4" type="ORF">DMAD_05137</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="synaptotagmin-5 isoform X2"/>
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    </dbReference>
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        <property type="entry name" value="synaptotagmin-5 isoform X2"/>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF378">
        <property type="entry name" value="SYNAPTOTAGMIN BETA, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SYNAPTOTAGMN"/>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0677">Repeat</keyword>
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        <location>
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            <end position="456"/>
        </location>
    </feature>
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        <location>
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            <end position="595"/>
        </location>
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        </location>
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        </location>
    </feature>
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            <end position="40"/>
        </location>
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        </location>
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        </location>
    </feature>
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            <end position="85"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF96515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="598" mass="64662" checksum="2D5C417FED8271DD" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FM84</accession>
    <name>A0AAU9FM84_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Prisilkin-39</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DMAD_05452</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FM84"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF96938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="113" mass="11470" checksum="E152487443BFB2BD" modified="2024-11-27" version="1">MVQCQYYGYPYYGGTGAGAGAGGGAGGGYGNGYGNAYGYGYPSYGGYPYYSYPSYNPYSIYGQYGQYGQSQFGYPSYGGYPYGGGNGMNTAYASASSSGGFASASAVSGGYYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FM86</accession>
    <name>A0AAU9FM86_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Collagen alpha-1(XVIII) chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05327</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00247">
        <property type="entry name" value="Endostatin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000137">
        <property type="entry name" value="Multiplexin collagen isoform Ap3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000048">
        <property type="entry name" value="Multiplexin collagen isoform Ap3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1620.70:FF:000001">
        <property type="entry name" value="Multiplexin collagen isoform Ap3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010515">
        <property type="entry name" value="Collagenase_NC10/endostatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045463">
        <property type="entry name" value="XV/XVIII_trimerization_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1082">
        <property type="entry name" value="COLLAGEN TRIPLE HELIX REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20010">
        <property type="entry name" value="Collagen_trimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06482">
        <property type="entry name" value="Endostatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0176">Collagen</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043964496" evidence="5">
        <location>
            <begin position="21"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="domain" description="Thrombospondin-like N-terminal" evidence="6">
        <location>
            <begin position="32"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="247"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="378"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="445"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="678"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="378"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="410"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="459"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="558"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="572"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="622"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF96760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1026" mass="108465" checksum="256CFC631AB6AC1B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FM87</accession>
    <name>A0AAU9FM87_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="22">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_05354</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:18106"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Mn(2+)(in) + ATP + H2O = Mn(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66820"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:66821"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Monomer. Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Membrane</location>
            <topology evidence="22">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Sarcoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="22"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FM87"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02085">
        <property type="entry name" value="P-type_ATPase_SPCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000008">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000006">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000017">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000106">
        <property type="entry name" value="E1-E2 ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006413">
        <property type="entry name" value="P-type_ATPase_IIA_PMR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01522">
        <property type="entry name" value="ATPase-IIA2_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
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    <sequence length="925" mass="102297" checksum="BD643E2263B47390" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Hexapoda</taxon>
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            <taxon>Endopterygota</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tomihara K."/>
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                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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    </dbReference>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="Uncharacterized protein, isoform B"/>
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    </dbReference>
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        <property type="entry name" value="FN3_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_sub2"/>
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        <property type="entry name" value="Striated_Muscle_Regulatory"/>
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        <property type="entry name" value="IG-LIKE AND FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN C25G4.10"/>
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        <location>
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        <location>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
    <sequence length="1655" mass="178469" checksum="BE3D9CA4FC64A0F3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTL0</accession>
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        <submittedName>
            <fullName evidence="13">Protein nervous wreck</fullName>
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    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07091</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
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            <taxon>Endopterygota</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="532" mass="58934" checksum="E0A9CECDA1D8E8FC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUC0</accession>
    <name>A0AAU9FUC0_DROMD</name>
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        <recommendedName>
            <fullName evidence="3">Cleavage and polyadenylation specificity factor subunit 6</fullName>
        </recommendedName>
    </protein>
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        <name evidence="10" type="ORF">DMAD_06934</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM CPSF6/7 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUC0"/>
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        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12643">
        <property type="entry name" value="RRM_CFIm68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CPSF6/7"/>
    </dbReference>
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        <property type="entry name" value="CPSF6/7_RSLD_N"/>
    </dbReference>
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        <property type="entry name" value="CPSF6_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23204">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25524">
        <property type="entry name" value="RSLD_CPSF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="93"/>
            <end position="173"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="440"/>
        </location>
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        <location>
            <begin position="502"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="187"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
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            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
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            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
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            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
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            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
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            <end position="384"/>
        </location>
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        <location>
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            <end position="402"/>
        </location>
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        </location>
    </feature>
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        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="8">
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            <begin position="525"/>
            <end position="534"/>
        </location>
    </feature>
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    <feature type="compositionally biased region" description="Low complexity" evidence="8">
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006265"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00016259"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF98900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="648" mass="70697" checksum="465B3BBBB507510B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUC5</accession>
    <name>A0AAU9FUC5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GATA zinc finger domain-containing protein 10-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_07230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUC5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF99292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="217" mass="26232" checksum="3B6AE79861D8DC04" modified="2024-11-27" version="1">MLNKSPLEAIESVAAARGHKIERENYLSSLRRLAKLDAESSEEPSQDTVYTSHYNKRHNWSEQAGGRQEHYQQQYHHHQQQHQPEGRYQQRYSQRGAHDRSQDDVRQSRWQSNSGGYRDHIRGRPQQEDRFGTSDNVWKQQQQQRGRQQYQDRSRDRITDWRRPQQQEQRTSGTYHGDFRSRNSDWNSTSDDPEWNKPHWNGHKETRRNQCDNQKYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUD2</accession>
    <name>A0AAU9FUD2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_07532</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="EMBL" id="AP029265">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:mRNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035613">
        <property type="term" value="F:RNA stem-loop binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16638">
        <property type="entry name" value="mRING-HC-C3HC3D_Roquin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1790:FF:000001">
        <property type="entry name" value="roquin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000004">
        <property type="entry name" value="roquin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000047">
        <property type="entry name" value="Roquin-2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041523">
        <property type="entry name" value="ROQ_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048575">
        <property type="entry name" value="Roquin_1_2-like_ROQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052249">
        <property type="entry name" value="Roquin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13139">
        <property type="entry name" value="RING FINGER AND CCCH-TYPE ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13139:SF54">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18386">
        <property type="entry name" value="ROQ_II"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUD4</accession>
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        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
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        <name evidence="14" type="ORF">DMAD_07496</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
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        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <location>
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        <location>
            <position position="63"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50219"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF99647.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUD7</accession>
    <name>A0AAU9FUD7_DROMD</name>
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        <submittedName>
            <fullName evidence="12">Protein boule</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_07094</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15 16">RF1</strain>
        </source>
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        </location>
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        </location>
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        <source>
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            <dbReference type="Pfam" id="PF02931"/>
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        <source>
            <dbReference type="Pfam" id="PF02932"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1011" mass="112354" checksum="E14DFBCC18AFE1C3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUG8</accession>
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        <recommendedName>
            <fullName evidence="12">Neurabin-1</fullName>
        </recommendedName>
        <alternativeName>
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        </alternativeName>
        <alternativeName>
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        <alternativeName>
            <fullName evidence="15">Protein phosphatase 1 regulatory subunit 9A</fullName>
        </alternativeName>
    </protein>
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        <name evidence="20" type="ORF">DMAD_07260</name>
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    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF99096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="263" mass="28346" checksum="BBB69B1D953BEDA9" modified="2024-11-27" version="1">MKVLVVFALALAYASASALRGVQTGPDGRITHGEDARTDQFPYQVGLSLKIGSSSAWCGGSLIGNEWVLTAAHCTDGVDSVEVYLGSTVRSSPKVKHHVTKDDIIIHADWNSRTLRNDISLIRIPRVEYSGAIHAVELPREESHYSSYDGDEVIASGWGRTSDNSSAVAAHLQYAHMKVISNSHCKRTYFTTIHESNICVSTPAGVSTCNGDSGGPLVLEHSKVQVGLTSFGSAAGCEKEYPAVFTRVTSYLGWIHEHTGISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUH3</accession>
    <name>A0AAU9FUH3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Polyubiquitin-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_07209</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUH3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010623">
        <property type="term" value="P:programmed cell death involved in cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000158">
        <property type="entry name" value="Polyubiquitin 5"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="153"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="229"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF99267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="307" mass="34463" checksum="BDB2521C7878AE95" modified="2024-11-27" version="1">MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUI8</accession>
    <name>A0AAU9FUI8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Protein nervous wreck</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07091</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUI8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051495">
        <property type="term" value="P:positive regulation of cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902905">
        <property type="term" value="P:positive regulation of supramolecular fiber organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11761">
        <property type="entry name" value="SH3_FCHSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000107">
        <property type="entry name" value="FCH and double SH3 domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000039">
        <property type="entry name" value="FCH and double SH3 domains protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000155">
        <property type="entry name" value="FCH and double SH3 domains protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035460">
        <property type="entry name" value="FCHSD_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF21">
        <property type="entry name" value="PROTEIN NERVOUS WRECK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="12">
        <location>
            <begin position="11"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="545"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="638"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="431"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="703"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="749"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="942"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="497"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="713"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="800"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="833"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="888"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="947"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="979"/>
            <end position="989"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF99111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1035" mass="113699" checksum="48E11D3A95971632" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUI9</accession>
    <name>A0AAU9FUI9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Kelch-like protein diablo</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_07370</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. May have a role in synapse differentiation and growth.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUI9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18459">
        <property type="entry name" value="BACK_KLHL20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18249">
        <property type="entry name" value="BTB_POZ_KLHL20_KLEIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000006">
        <property type="entry name" value="Kelch-like family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000001">
        <property type="entry name" value="Kelch-like family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF451">
        <property type="entry name" value="KELCH-LIKE PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="9">
        <location>
            <begin position="74"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF99463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="622" mass="68572" checksum="833AB59E45D15B7F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FUJ2</accession>
    <name>A0AAU9FUJ2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DMAD_07551</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FUJ2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF99722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="115" mass="13131" checksum="AF892529D1ADE641" modified="2024-11-27" version="1">MLPSALLTLRMLGQLEEKEQKDAEELYENCTRSWRDQFKNPNMANYAIGMEFMSQCRRSPVSSGRSFSLYSDNGNSSIETPRLFQSYLSDPLAISDVEKHSALITGKSLSRRCRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5L1</accession>
    <name>A0AAU9G5L1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Patronin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_02937</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The CKK domain binds microtubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CAMSAP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036449">
        <property type="term" value="C:microtubule minus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030507">
        <property type="term" value="F:spectrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.360:FF:000002">
        <property type="entry name" value="Patronin, isoform M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.360">
        <property type="entry name" value="CKK domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032940">
        <property type="entry name" value="CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031372">
        <property type="entry name" value="CAMSAP_CC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058042">
        <property type="entry name" value="CAMSAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014797">
        <property type="entry name" value="CKK_CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038209">
        <property type="entry name" value="CKK_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595:SF0">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17095">
        <property type="entry name" value="CAMSAP_CC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08683">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25532">
        <property type="entry name" value="CH_CAMSAP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01051">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51508">
        <property type="entry name" value="CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="157"/>
            <end position="289"/>
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        <source>
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        </source>
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</entry>
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        <name type="scientific">Drosophila madeirensis</name>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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        <source>
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</entry>
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        <name type="scientific">Drosophila madeirensis</name>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <strain evidence="19 20">RF1</strain>
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        </subcellularLocation>
    </comment>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51220">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50993">
        <property type="entry name" value="NIDOGEN_G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043919573" evidence="15">
        <location>
            <begin position="28"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="domain" description="NIDO" evidence="18">
        <location>
            <begin position="114"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Nidogen G2 beta-barrel" evidence="17">
        <location>
            <begin position="332"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="552"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="598"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="809"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="852"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="975"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="13">
        <location>
            <begin position="1104"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="13">
        <location>
            <begin position="1147"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="13">
        <location>
            <begin position="1191"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="642"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="651"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="669"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="819"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="863"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="985"/>
            <end position="1002"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFG04043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1373" mass="151397" checksum="7C9E1892E2BD332A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6C2</accession>
    <name>A0AAU9G6C2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lysyl oxidase homolog 3B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_02863</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6C2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004720">
        <property type="term" value="F:protein-lysine 6-oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lysyl oxidase-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="LOX-like_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001695">
        <property type="entry name" value="Lysyl_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45817">
        <property type="entry name" value="LYSYL OXIDASE-LIKE-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45817:SF4">
        <property type="entry name" value="LYSYL OXIDASE-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01186">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SRCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00074">
        <property type="entry name" value="LYSYLOXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043750996" evidence="3">
        <location>
            <begin position="19"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="4">
        <location>
            <begin position="50"/>
            <end position="150"/>
        </location>
    </feature>
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            <begin position="180"/>
            <end position="286"/>
        </location>
    </feature>
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            <begin position="75"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
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            <begin position="88"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
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            <end position="129"/>
        </location>
    </feature>
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        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFG03664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="498" mass="56493" checksum="A5CD8199604FBF79" modified="2024-11-27" version="1">MVPRLLCVTVLWCHLAAGVVQHRSLEDARLERQRLVHKYIKALNKEEGAIRLVGGDNEYEGNIEVLHNGKWGAICDDEWDATEGHIVCRQLGFPGMSRYTHSGYFGPARRRFWMDNLFCEGHEQELVECHFEGWGENDCEPGEAAGVVCNPPAGTPIPEPLLREEDIPKFPIHTRSRLYVQLRGGRERNEGRVEVRLDGGRWGSICADGWSLLEANVVCRQLGLGYAREAFQTDYFGGFNVSRPVLSGSECYGNETELADCLHHDPIRGIVSCHGSRQHVAAVICDYSSPDLVIDYLEIEQTAHLEDRPMLLMQCAMEENCVASEAYQIQRDDPHWRYRTRRLLKFTAAAINAGNADFRPFKEKSQWEWHMCHMHFHSMEVFATFDIFDLSGRKVAQGHKASFCLEDSTCLPGVSKKYNCANYGDQGISINCSDVYLYNLDCQWVDVTDLSPGSYILKIAINPEFKVSEMSFDNNAAICDLIYTENHARVQNCQLARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6C6</accession>
    <name>A0AAU9G6C6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Atrophin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_02726</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6C6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG03480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="157" mass="16781" checksum="F72D76ACE0077B20" modified="2024-11-27" version="1">MDDSGIYWILFFTFSVFFLTSVAAFLTRGRNQRAQLTKCAAPVVVTSATHTAPGGYPVTQLPPPGYPAGNAYAVPYPAQTTGNVTVQMPMPMQQQHQQMPMPMPGTHTHGVAYPPYPGAGAANMNPPTYDMAVASPGPSVMPAGYEKQSPYNPNFGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6C8</accession>
    <name>A0AAU9G6C8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Putative inorganic phosphate cotransporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_02758</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">May be an inorganic phosphate cotransporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sodium/anion cotransporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6C8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="MFS_SLC17"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Picot, isoform B"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Solute carrier family 17 member 3"/>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
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    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
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    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF280">
        <property type="entry name" value="FI21844P1-RELATED"/>
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        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="106"/>
            <end position="126"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="14">
        <location>
            <begin position="35"/>
            <end position="455"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008586"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00068450"/>
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    </evidence>
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        <source>
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            <dbReference type="PROSITE" id="PS50850"/>
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    </evidence>
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            <dbReference type="EMBL" id="BFG03523.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="491" mass="53290" checksum="71ECE4C6D8729B0C" modified="2024-11-27" version="1">MVYLVNGERTPLLLRVVPNHIKTLASSGFGVRHWQAILLFVGMMINYFQRVDISAAIVPMTQSTAGAPSYNWNVSEKSLILSSFFWGYVVSQVPAGLLAKRFGAKIVLGTATAVGGVLSFFHPMAAASGWESVCVLRILTGLVQGTVYPCVHTLLAKWVPRTERGFLTTGIYSGAQFGTALILVTSGFIFESSLGWPGLFHLSGGLSLAWALLFFWQAANEPATARRITKTEQEYIESLTGSNSSSQSMPVPWIAIFKSPAFYGLLAGHCGFAWGFYTLLTQMPTYMNKVLHLDVKSNAFLSSLPYLAMGVLCLVVSPISDVLTNRGFISITTARKLFNSIGQWGPMACLIGLGYMTADQKTWAILLLTLAVGTNAGCSCGYLINHIDLSPNFAGPMMGVTNGIAGVTSIVAPLVVGAILTEEEDPNQWRLVFFITGAIYLVCNTLFVIFGKATVQFWNEPTSMSSTIALRNNFENDSQTIAPTTVKDNRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6C9</accession>
    <name>A0AAU9G6C9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Vigilin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_02541</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22405">
        <property type="entry name" value="KH-I_Vigilin_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22413">
        <property type="entry name" value="KH-I_Vigilin_rpt10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22414">
        <property type="entry name" value="KH-I_Vigilin_rpt11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22415">
        <property type="entry name" value="KH-I_Vigilin_rpt12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22416">
        <property type="entry name" value="KH-I_Vigilin_rpt13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22417">
        <property type="entry name" value="KH-I_Vigilin_rpt14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22418">
        <property type="entry name" value="KH-I_Vigilin_rpt15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22406">
        <property type="entry name" value="KH-I_Vigilin_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22407">
        <property type="entry name" value="KH-I_Vigilin_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22408">
        <property type="entry name" value="KH-I_Vigilin_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22409">
        <property type="entry name" value="KH-I_Vigilin_rpt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02394">
        <property type="entry name" value="KH-I_Vigilin_rpt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22410">
        <property type="entry name" value="KH-I_Vigilin_rpt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22411">
        <property type="entry name" value="KH-I_Vigilin_rpt8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22412">
        <property type="entry name" value="KH-I_Vigilin_rpt9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="High density lipoprotein binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000061">
        <property type="entry name" value="High density lipoprotein binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000056">
        <property type="entry name" value="Tudor and KH domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000152">
        <property type="entry name" value="Vigilin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000018">
        <property type="entry name" value="vigilin isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000039">
        <property type="entry name" value="vigilin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057778">
        <property type="entry name" value="KH_Vigilin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627:SF31">
        <property type="entry name" value="DODECA-SATELLITE-BINDING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627">
        <property type="entry name" value="SCP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24668">
        <property type="entry name" value="KH_Vigilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="162"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="234"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="307"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="376"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="445"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="516"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="590"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="662"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="736"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="809"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="882"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="989"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="1067"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="1140"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="70"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="920"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1265"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="639"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="786"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="96"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1270"/>
            <end position="1283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG03237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1308" mass="144361" checksum="B6C35E3313AF2EB5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6D1</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">DMAD_02456</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6D1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG03125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="548" mass="59478" checksum="815A5AA31AA10BE6" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6D2</accession>
    <name>A0AAU9G6D2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Endothelin-converting enzyme 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_03070</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M13 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6D2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_M13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008753">
        <property type="entry name" value="Peptidase_M13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF241">
        <property type="entry name" value="GH26575P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01431">
        <property type="entry name" value="Peptidase_M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05649">
        <property type="entry name" value="Peptidase_M13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00786">
        <property type="entry name" value="NEPRILYSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043684111" evidence="9">
        <location>
            <begin position="21"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 N-terminal" evidence="11">
        <location>
            <begin position="112"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 C-terminal" evidence="10">
        <location>
            <begin position="426"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG03977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="622" mass="71476" checksum="9EBBFA374917520F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6D3</accession>
    <name>A0AAU9G6D3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ran-binding proteins 9/10 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_03113</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RANBP9/10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G6D3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="CDD" id="cd12909">
        <property type="entry name" value="SPRY_RanBP9_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000011">
        <property type="entry name" value="RAN binding protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="CRA_dom"/>
    </dbReference>
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        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035782">
        <property type="entry name" value="SPRY_RanBP9/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="3">
        <location>
            <begin position="290"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="CTLH" evidence="4">
        <location>
            <begin position="546"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="213"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="625"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="706"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="145"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="171"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="636"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="710"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG04058.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 14"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47595:SF1">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myb_DNA-bind_4"/>
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    </dbReference>
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            <begin position="90"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="214"/>
            <end position="302"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
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        <location>
            <begin position="309"/>
            <end position="338"/>
        </location>
    </feature>
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        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
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        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG03719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="424" mass="48143" checksum="A0D96BBBCDD780C8" modified="2024-11-27" version="1">MSKHSVPFEENNEDDHEPEQKYARLLSPGSGLALDNGHGRSRSVSGRKSRKKDPETDSNLSSYDEEVALPTDTDGEDAKDASSSMPSNVRCKWAEGEVDVFLDLITSMGLQAALLRKRNAKVFKLLSKEMAKRQYIKGPDKLRIKFQLLRRLYNKAKNGGDTFEYYEAMRKLLDPTEAELSAVDAKPILSSGSESESNDSDDDDGDISQRGGAHFWTDEEVDAFLLIIKENGFFRALDGSKKRNFQTLVHISNILAKQSFKRTPHQLRNKLRLLIKRYREAQTEGLSNVRILPRHFQLFDELVNAPKDLTRRSSQKPARSATARTDLLDSDSESSTSSCDLLRAAGESDDFEIATEPTPLEVLTSISEGQKKLVEYLKQSNDNFLRQQRDMQKQFLQDVTELMRQDREETLRRISEMLEGPKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6D6</accession>
    <name>A0AAU9G6D6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Potassium channel subfamily T member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_03107</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="11">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="BFG04045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005228">
        <property type="term" value="F:intracellular sodium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Potassium channel subfamily T member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Slowpoke 2, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF10">
        <property type="entry name" value="SLOWPOKE 2, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
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    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="283"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="14">
        <location>
            <begin position="325"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
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            <end position="696"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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            <end position="1144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1382"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="674"/>
            <end position="694"/>
        </location>
    </feature>
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        <location>
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            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1123"/>
            <end position="1138"/>
        </location>
    </feature>
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        <location>
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            <end position="1406"/>
        </location>
    </feature>
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        <location>
            <begin position="1407"/>
            <end position="1444"/>
        </location>
    </feature>
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        <location>
            <begin position="1445"/>
            <end position="1469"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG04045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1469" mass="162366" checksum="AFF787533EF00636" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6G3</accession>
    <name>A0AAU9G6G3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Laccase-5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_02436</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000073">
        <property type="entry name" value="Laccase 2, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF232">
        <property type="entry name" value="STRAW, ISOFORM G"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="208"/>
            <end position="280"/>
        </location>
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    <feature type="domain" description="Plastocyanin-like" evidence="5">
        <location>
            <begin position="313"/>
            <end position="462"/>
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    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="586"/>
            <end position="720"/>
        </location>
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            <begin position="20"/>
            <end position="45"/>
        </location>
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        <location>
            <begin position="62"/>
            <end position="106"/>
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    </feature>
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            <begin position="29"/>
            <end position="43"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00010609"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFG03101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="745" mass="82158" checksum="8569626416EA7333" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6G9</accession>
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        <submittedName>
            <fullName evidence="15">Kinesin-like protein KIF13A</fullName>
        </submittedName>
    </protein>
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        <name evidence="15" type="ORF">DMAD_02593</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
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        <property type="protein sequence ID" value="BFG03301.1"/>
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        <property type="component" value="Chromosome E"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:microtubule motor activity"/>
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    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:microtubule-based movement"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
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    </dbReference>
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        <property type="entry name" value="Kinesin family member 13A"/>
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    </dbReference>
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        <property type="entry name" value="Kinesin family member 13A"/>
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    </dbReference>
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        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
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        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
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        <property type="entry name" value="Kinesin_motor_CS"/>
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        <property type="entry name" value="Kinesin_motor_dom_sf"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
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        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="5"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="14">
        <location>
            <begin position="1851"/>
            <end position="1893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="829"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1483"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1600"/>
            <end position="1623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1651"/>
            <end position="1729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1758"/>
            <end position="1787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1908"/>
            <end position="1942"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="589"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1483"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1493"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1651"/>
            <end position="1661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1695"/>
            <end position="1712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1718"/>
            <end position="1729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1767"/>
            <end position="1782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1915"/>
            <end position="1928"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="100"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG03301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1942" mass="217592" checksum="1411385412890C7D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6H2</accession>
    <name>A0AAU9G6H2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Synaptobrevin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_03096</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9G6H2"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="R-SNARE_VAMP2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="5">
        <location>
            <begin position="25"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFG04022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="138" mass="14818" checksum="4BA57CD0B82130E7" modified="2024-11-27" version="1">MENNEAPSPSGSNANDNNNAAAQKRLQQTQAKVDEVVGIMRVNVEKVLERDQKLSELGERADQLEQGASQFEQQAGKLKRKQWWANMKMMIILGVIAVVLLIIVLVSVWPESSGSSSSGGGGAKSITEPLPVTGAPPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G6H4</accession>
    <name>A0AAU9G6H4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="34">Protocadherin-like wing polarity protein stan</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="34" type="ORF">DMAD_03134</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="34 35" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="34 35">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:cell-cell junction"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:axon guidance"/>
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        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:establishment of imaginal disc-derived wing hair orientation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:positive regulation of neurogenesis"/>
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        <property type="term" value="P:regulation of tube length, open tracheal system"/>
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        <property type="match status" value="8"/>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
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    </dbReference>
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        <property type="entry name" value="Starry night, isoform B"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
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        <property type="entry name" value="AGRL2-4_GAIN_subdom_A"/>
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        <property type="entry name" value="Cadherin-like_dom"/>
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        <property type="entry name" value="Cadherin-like_sf"/>
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        <property type="entry name" value="Cadherin_CELSR1-3_9th"/>
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        <property type="entry name" value="Cadherin_CS"/>
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        <property type="entry name" value="ConA-like_dom_sf"/>
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        <property type="entry name" value="GPCR_2_secretin-like"/>
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        <property type="entry name" value="LE_dom"/>
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        <property type="entry name" value="FAT ATYPICAL CADHERIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="match status" value="9"/>
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        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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    </dbReference>
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        <property type="entry name" value="EGF_1"/>
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</entry>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
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            <taxon>Endopterygota</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2131" mass="239782" checksum="3F3616E04C968C3D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EYX6</accession>
    <name>A0AAU9EYX6_DROMD</name>
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        <submittedName>
            <fullName evidence="15">Octopamine receptor Oamb</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_09811</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9EYX6"/>
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        <property type="component" value="Chromosome O"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15063">
        <property type="entry name" value="7tmA_Octopamine_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000351">
        <property type="entry name" value="Octopamine receptor Oamb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000369">
        <property type="entry name" value="octopamine receptor Oamb isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF174">
        <property type="entry name" value="TYRAMINE_OCTOPAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="515"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="548"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="37"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="471"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="616"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="365"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="624"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF91562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="640" mass="71302" checksum="DD633F7DDF7FD3FA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZ86</accession>
    <name>A0AAU9EZ86_DROMD</name>
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        <recommendedName>
            <fullName evidence="10">Inhibitor of growth protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07888</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ING family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EZ86"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16857">
        <property type="entry name" value="ING_ING1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000021">
        <property type="entry name" value="Inhibitor of growth 2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028643">
        <property type="entry name" value="ING1_PHD_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
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    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
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    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
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    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF89">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 8 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="12">
        <location>
            <begin position="353"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="198"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="152"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="355"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="370"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF89046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="428" mass="45688" checksum="DE9F4D689B88216B" modified="2024-11-27" version="1">MINPIASESEAINSATYVDNYIDSVENLPDDVQRQLSRIRDIDVQYRGLIRDVDHYYDLYLTLQNSSDSSRRSRSMNLMHQCLIQAQELGDEKMQIVGHMQEIIDGKLRQLDTNQQNLDLKEERDRYTLMDDCPTSKLQRLQSPMRDQANTVGGGAGSSSGLNGNGLGGSVGGFTVKDQYGLPVYVGVVGVTNPGTIISGNGGGSTPNSERSSHGVSNGGNGSNGNLSASGGGDLQRGGSKRSRRRNESLVNGSSLEMGGNESNSANEAGGSGSGNGERKGSLGSAGAGQRKGNMQGASGGSLASGSAGTSSGGGNNSGKKKKRKVRGAGASNTTANAREETPPPETIDPDEPTYCVCNQISFGEMILCDNDLCPIEWFHFSCVSLVLKPKGKWFCPNCRGERPNVMKPKAQFLKELERYNKEKEEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZ93</accession>
    <name>A0AAU9EZ93_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Biotin--protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_08476</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the biotin--protein ligase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EZ93"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003142">
        <property type="entry name" value="BPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02237">
        <property type="entry name" value="BPL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="4">
        <location>
            <begin position="813"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="338"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="343"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF89804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1079" mass="118711" checksum="ED2FB774DB8352CE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZ95</accession>
    <name>A0AAU9EZ95_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein unc-13 homolog 4B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_09932</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the unc-13 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EZ95"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099503">
        <property type="term" value="C:secretory vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010439">
        <property type="entry name" value="MUN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014770">
        <property type="entry name" value="Munc13_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014772">
        <property type="entry name" value="Munc13_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052095">
        <property type="entry name" value="UNC-13_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45999:SF2">
        <property type="entry name" value="PROTEIN UNC-13 HOMOLOG 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45999">
        <property type="entry name" value="UNC-13-4A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06292">
        <property type="entry name" value="MUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51258">
        <property type="entry name" value="MHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51259">
        <property type="entry name" value="MHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHD1" evidence="9">
        <location>
            <begin position="6"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="MHD2" evidence="10">
        <location>
            <begin position="238"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="334"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF91712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="496" mass="57434" checksum="1899FA28608BBCCB" modified="2024-11-27" version="1">MGTTLFELYLVLKRYVQLGESLCTDELEMSGFYQWFETGVTHWLDISIIKALNRIQKAIDLDQLVAVDETVKYSSSAVDTLSIFYQIKIFWQQLDWPEVEGSYTFIAKIVNDLCRCCIFYAQRMSQRVEKLTKLGDNNKMFILSEEWCLAINNMDYIRQSLPSFIKELGIDNVIQKLGEYRTNLEADRCSSTIHAVIENALDTERNQIVELIEIVAHKMAPPMKLYLAEGAEVLHKDSNSMDQLMMYLESSLDTLYNTLNEVNFGRILESIWSELSIILYDLIQSNLDKRRPPAYFKNLKETLQTMIYCFKMADLIHQYYMERLEYQKNQPLSPFGQLTIRAEFTDSSLLLTILNARNLLPMDSNGLVDSFTKASFMPTIRFNGVSPVKTTVHHKNCFPLYDQEFCIILNEQQMAAGDSLILFSVKDKDLFGMSSQYIGECYISFTDLRESDGEQIIMNLSRPEYTDSDSLRALEFRQGDKQAKDFIKKLKSKSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZ98</accession>
    <name>A0AAU9EZ98_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Ankyrin repeat and KH domain-containing protein mask</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_09492</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22404">
        <property type="entry name" value="KH-I_MASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000046">
        <property type="entry name" value="Ankyrin repeat and KH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000804">
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="17 18">RF1</strain>
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            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="11">Belongs to the chitin synthase family. Class IV subfamily.</text>
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</entry>
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</entry>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF91889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1202" mass="133341" checksum="47EF317A0AE377F6" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F2L7</accession>
    <name>A0AAU9F2L7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Metalloendopeptidase</fullName>
            <ecNumber evidence="19">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_10076</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="18 19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="18 19">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="19"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008586">
        <property type="term" value="P:imaginal disc-derived wing vein morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032927">
        <property type="term" value="P:positive regulation of activin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030513">
        <property type="term" value="P:positive regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd04281">
        <property type="entry name" value="ZnMc_BMP1_TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000004">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000052">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000053">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000003">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000240">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015446">
        <property type="entry name" value="BMP_1/tolloid-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034036">
        <property type="entry name" value="ZnMP_TLD/BMP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF43">
        <property type="entry name" value="TOLLOID-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001199">
        <property type="entry name" value="BMP_1/tolloid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 18 19" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5 18 19" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="10 16 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="23">
        <location>
            <begin position="519"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="21">
        <location>
            <begin position="723"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="21">
        <location>
            <begin position="840"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="954"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="21">
        <location>
            <begin position="997"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="1114"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="21">
        <location>
            <begin position="1158"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="21">
        <location>
            <begin position="1271"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="64"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="242"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="389"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="389"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="405"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="425"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="active site" evidence="15 18">
        <location>
            <position position="615"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="614"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="618"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="624"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="584"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="586"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001199-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001199-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="BFF91904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1455" mass="163966" checksum="8E56A1244E0F1AAF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F2M8</accession>
    <name>A0AAU9F2M8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">UDP-glucuronosyltransferase 2B1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_08790</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F2M8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF159">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF90229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="133" mass="15210" checksum="CB4B132A9B9F02C5" modified="2024-11-27" version="1">MNVERARRIGFGLGLDLHQLKQEELEAAIHKLLSEPSYAKASSQISERYRDQPQPSLERAIWWTEYIIRHQGAPHLRAASRDLNYIQLHSLDTLFVLVGVPLLIVGLLLKLSCRLLRGSKPKSCPFADKQKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCG8</accession>
    <name>A0AAU9FCG8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger protein 93</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_11231</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FCG8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002737">
        <property type="entry name" value="AGAP008430-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002467">
        <property type="entry name" value="Zinc finger protein 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF416">
        <property type="entry name" value="FI01424P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="385"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="413"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="441"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="497"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="288"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF93367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="558" mass="60929" checksum="6CD43108A919FC83" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCH6</accession>
    <name>A0AAU9FCH6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein phosphatase PP2A regulatory subunit A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_11218</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit A family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FCH6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000011">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit A alpha isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF4">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="14"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="91"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="168"/>
            <end position="206"/>
        </location>
    </feature>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">RF1</strain>
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</entry>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    </evidence>
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        </source>
    </evidence>
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</entry>
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        <lineage>
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                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <organism evidence="4 5">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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    </dbReference>
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        <location>
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        <source>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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            <strain evidence="4 5">RF1</strain>
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        <text evidence="1">Belongs to the EEIG family.</text>
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    </dbReference>
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            <end position="154"/>
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            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="775"/>
            <end position="810"/>
        </location>
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            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
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            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
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            <begin position="782"/>
            <end position="810"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00034780"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
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        <source>
            <dbReference type="EMBL" id="BFF93553.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="875" mass="95662" checksum="8FFB445FCA6A34FE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="entry name" value="NAV1-like_ubiquitin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12784:SF28">
        <property type="entry name" value="PROTEIN SICKIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12784">
        <property type="entry name" value="STEERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_lid_NAV1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="1933"/>
        </location>
    </feature>
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        </location>
    </feature>
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    </feature>
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    </feature>
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    </feature>
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            <end position="1228"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1464"/>
            <end position="1491"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
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            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
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            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
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            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="4">
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    <feature type="compositionally biased region" description="Low complexity" evidence="4">
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        </location>
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        </location>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2103" mass="223081" checksum="27C24790105DC73F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKX1</accession>
    <name>A0AAU9FKX1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Probable aminoacyl tRNA synthase complex-interacting multifunctional protein 2</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="ORF">DMAD_05078</name>
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    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AIMP2_thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13438">
        <property type="entry name" value="AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13438:SF2">
        <property type="entry name" value="AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18569">
        <property type="entry name" value="Thioredoxin_16"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIMP2 thioredoxin-like" evidence="6">
        <location>
            <begin position="127"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF96413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="324" mass="36237" checksum="08BDA634570F34FF" modified="2024-11-27" version="1">MYELKTLLPQFDIKLPTCMYNLKNVSLSADPTTSTSSVSCSASEFSSSINSRKSIPCALDLDFLGRQIQHLLKDDTVSVAIRQDKIIQQLQGLKTQLGKIRAGLGICGKTVQHTTAFQNGRLKEVPLQDIVINGHPNFIPYALLALKNVWRALYTVNVKTFTHSTMADIGPAAREFEAEMDKIPVNNALPNINVTLIWKNCEHTEMISSPTMYLPIYGEVNVIRYLGRVGPAEYRYEGSPLCNEIDLVLDICYQLLRCSTHKAQVAMVRLLDQRLKQQYFGGSQMSVADIGVHSSLIRIPTITDKDLTPALLAWRKRAKLVTQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKY1</accession>
    <name>A0AAU9FKY1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptide-methionine (S)-S-oxide reductase</fullName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Peptide-methionine (S)-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05095</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKY1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1060.10:FF:000004">
        <property type="entry name" value="Peptide methionine sulfoxide reductase A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049006">
        <property type="entry name" value="MsrA_helical"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774:SF1">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE MSRA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20939">
        <property type="entry name" value="MsrA_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="5">
        <location>
            <begin position="40"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Selenoprotein methionine sulfoxide reductase A helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF96454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="245" mass="27908" checksum="E57F7EAB9AA67418" modified="2024-11-27" version="1">MRWLLRFGYSLKFMFLRTVRNEQKELSILPVHHVNIPKATATFGMGCFWGAESLYGATRGVLRTTVGYAGGSSELPTYRKLGDHTEVLEIDYDPTVISFKELLDLFWNNHEYGLTTPIKRQYASLILYHDDEQKKMAVTSKLEEQERRAPELITTEVAPKVNFYAAEAYHQKYRLQGHKDLATSLNLSPTLLQSSYVATKLNGYLAGVGGMEQFKSEVDTLGLTPTQRQYCSYHVEQNEGQGLYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKY3</accession>
    <name>A0AAU9FKY3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Solute carrier family 12 member 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_13537</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC12A transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKY3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015379">
        <property type="term" value="F:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000013">
        <property type="entry name" value="Solute carrier family 12 member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF72">
        <property type="entry name" value="GH08340P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="370"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="482"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="505"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="77"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="11">
        <location>
            <begin position="578"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF96310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="939" mass="103546" checksum="04037740B3F5B850" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FKY7</accession>
    <name>A0AAU9FKY7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fat-body protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_05199</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FKY7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097009">
        <property type="term" value="P:energy homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1520">
        <property type="entry name" value="Hemocyanin, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1370.10">
        <property type="entry name" value="Hemocyanin, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013788">
        <property type="entry name" value="Hemocyanin/hexamerin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005203">
        <property type="entry name" value="Hemocyanin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037020">
        <property type="entry name" value="Hemocyanin_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005204">
        <property type="entry name" value="Hemocyanin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036697">
        <property type="entry name" value="Hemocyanin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511:SF5">
        <property type="entry name" value="FAT-BODY PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511">
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL90</accession>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <location>
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            <end position="791"/>
        </location>
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        <source>
            <dbReference type="EMBL" id="BFF96321.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL92</accession>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </feature>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF18569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF96415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="291" mass="32593" checksum="53712F068AD2CC1D" modified="2024-11-27" version="1">MYELKTLLPQFDIKLPTCMYNLKNVSLSADPTTSTSSVSCDDTVSVAIRQDKIIQQLQGLKTQLGKIRAGLGICGKTVQHTTAFQNGRLKEVPLQDIVINGHPNFIPYALLALKNVWRALYTVNVKTFTHSTMADIGPAAREFEAEMDKIPVNNALPNINVTLIWKNCEHTEMISSPTMYLPIYGEVNVIRYLGRVGPAEYRYEGSPLCNEIDLVLDICYQLLRCSTHKAQVAMVRLLDQRLKQQYFGGSQMSVADIGVHSSLIRIPTITDKDLTPALLAWRKRAKLVTQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL93</accession>
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        <submittedName>
            <fullName evidence="10">Restin homolog</fullName>
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        <name evidence="10" type="ORF">DMAD_13590</name>
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    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10 11">RF1</strain>
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            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
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        <property type="protein sequence ID" value="BFF96378.1"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
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        <property type="entry name" value="CLIP1_ZNF"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF93">
        <property type="entry name" value="RESTIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CLIP1_ZNF"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="215"/>
            <end position="257"/>
        </location>
    </feature>
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        <location>
            <begin position="331"/>
            <end position="373"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <end position="290"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
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        </location>
    </feature>
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            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1432"/>
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        </location>
    </feature>
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        </location>
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        <location>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF96378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1799" mass="201838" checksum="007275D1E6807807" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FL96</accession>
    <name>A0AAU9FL96_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein cappuccino</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_13557</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family. Cappuccino subfamily.</text>
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        <property type="protein sequence ID" value="BFF96336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome U"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <text>hydrogencarbonate(in) + chloride(out) = hydrogencarbonate(out) + chloride(in)</text>
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                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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</entry>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Yamamoto D."/>
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        <name evidence="2" type="ORF">DMAD_07174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTP0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16021">
        <property type="entry name" value="ALP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000017">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004245">
        <property type="entry name" value="DUF229"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974:SF9">
        <property type="entry name" value="DUF229 DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974">
        <property type="entry name" value="FI08016P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02995">
        <property type="entry name" value="DUF229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF99218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="679" mass="77212" checksum="6D581261D27D4A18" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTP5</accession>
    <name>A0AAU9FTP5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">choline-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07017</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.15" evidence="10"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTP5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004105">
        <property type="term" value="F:choline-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000016">
        <property type="entry name" value="Putative choline-phosphate cytidylyltransferase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045049">
        <property type="entry name" value="Pcy1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739:SF13">
        <property type="entry name" value="CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739">
        <property type="entry name" value="CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="94"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="359"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF99009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="378" mass="42899" checksum="F60916D266E37EB0" modified="2024-11-27" version="1">MFEPESPTTSGRSTPFHDFETDQQLEDNSTDSESGQCPVLSLYDLNFEKYPFMKPCPYDNDVQALDELEACDYTQRITYEMACAGQTKRRVRIYADGIYDLFHQGHARQLMQTKNLFPNVYLLVGVCSTELTLKMKGRTVMNGLERYEAVRHCRYVDEVVANAPWTLTDEFLNEHKIDFVAHDDIPYGGAGGVGDIYAHLKERGMFVATERTPGVSTSDIVARIVKDYDLFVRRNLARGYTAKELNVSFLSEKKFRLQNKMDELKDRGRRVKVDLQARWEAKSRDLIKAFLQAFGRERLFSIWRESKGKIMSTWGSNTSLAAESFFDSDQDDSEDEYMDAMSASDSVLEAARVHAAAAENANNLSRSSSSPEVGSGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTP8</accession>
    <name>A0AAU9FTP8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA-binding protein D-ETS-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_07309</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTP8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000343">
        <property type="entry name" value="Ets at 65A, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF304">
        <property type="entry name" value="DNA-BINDING PROTEIN D-ETS-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ETS" evidence="7">
        <location>
            <begin position="343"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="195"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="296"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="221"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF99392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="516" mass="54864" checksum="D7728C18D52703FA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU03</accession>
    <name>A0AAU9FU03_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neurabin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Neurabin-I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Neural tissue-specific F-actin-binding protein I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Protein phosphatase 1 regulatory subunit 9A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_07260</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06790">
        <property type="entry name" value="PDZ_neurabin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09512">
        <property type="entry name" value="SAM_Neurabin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000010">
        <property type="entry name" value="Neurabin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000008">
        <property type="entry name" value="Neurabin-1 isoform 1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040645">
        <property type="entry name" value="Neurabin-1/2_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043446">
        <property type="entry name" value="Neurabin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16154">
        <property type="entry name" value="NEURABIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16154:SF6">
        <property type="entry name" value="SPINOPHILIN, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17817">
        <property type="entry name" value="PDZ_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="1304"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="18">
        <location>
            <begin position="2127"/>
            <end position="2171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="81"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="259"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="646"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="975"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1055"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1428"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1701"/>
            <end position="1763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1913"/>
            <end position="1972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1985"/>
            <end position="2006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="2035"/>
            <end position="2106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="2192"/>
            <end position="2219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="1500"/>
            <end position="1608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="358"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="424"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="440"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="459"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="493"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="509"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="975"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="992"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1057"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1434"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1703"/>
            <end position="1719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1745"/>
            <end position="1761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1919"/>
            <end position="1932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1985"/>
            <end position="1997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="2050"/>
            <end position="2070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="2081"/>
            <end position="2106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF99338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2219" mass="240786" checksum="0E4467F8252140D8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU06</accession>
    <name>A0AAU9FU06_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IST1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Charged multivesicular body protein 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_06989</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">ESCRT-III-like protein involved in cytokinesis, nuclear envelope reassembly and endosomal tubulation. Is required for efficient abscission during cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with CHMP1A, CHMP1B, VPS4A and VTA1. Interacts with SPAST, STAMBP, and USP8. May interact with VPS37B. May associate with the ESCRT-I complex. Interacts with MITD1, in competition with VSP4. Interacts with SPART (via MIT domain); leading to the recruitment of SPART to midbodies. Interacts with SPAST.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the IST1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF98971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome J"/>
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    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.60:FF:000001">
        <property type="entry name" value="IST1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.60">
        <property type="entry name" value="Vacuolar protein sorting-associated protein Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005061">
        <property type="entry name" value="Ist1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042277">
        <property type="entry name" value="IST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161">
        <property type="entry name" value="IST1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161:SF5">
        <property type="entry name" value="IST1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03398">
        <property type="entry name" value="Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="240"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="8"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="293"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="375"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFF98971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="387" mass="42672" checksum="36830840D0D71131" modified="2024-11-27" version="1">MFSSGPNYNKLKTNLRLALNRLKLLEKKKAELTQKSRKEIADYLATGKTERARIRVEHIIREDYLVEAMEMVEMYCDLLLARFGLITQMKELDSGIAEPVASLVWVCPRLQSDIAELKIISDIFITKYGPQFAEHNRTATGDHSVSEKLMHKLTLQAPPKLLVENYLIAIAKNYNIEYEPDPQVMQEEQRPEHHLIDLSDRNNLSGSGGPPPQMGFIGYPAVPPLPNMPMPPTGKPFNYPPFGGGGGGGGGAAACAGPQPPPPFAYNIPPNQPPQPTMPSMVPGKCAEEKDLNTNFINRETNQTDDSGSPDENILNANKPKPQPRSKLPPGGPTLPSAPPALDLPSLPNVPLDLPDIPPGPAGKKEDEDEIDFDELSRRFENLKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU10</accession>
    <name>A0AAU9FU10_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Spectrin beta chain non-erythrocytic 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_07437</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the spectrin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21193">
        <property type="entry name" value="CH_beta_spectrin_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21194">
        <property type="entry name" value="CH_beta_spectrin_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10571">
        <property type="entry name" value="PH_beta_spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000001">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000250">
        <property type="entry name" value="Blast:Spectrin beta chain, non-erythrocytic 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000374">
        <property type="entry name" value="Blast:Spectrin beta chain, non-erythrocytic 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000200">
        <property type="entry name" value="Karst, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000303">
        <property type="entry name" value="Karst, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000210">
        <property type="entry name" value="Karst, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000288">
        <property type="entry name" value="Karst, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000007">
        <property type="entry name" value="Spectrin alpha chain non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000013">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000017">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000011">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000024">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000043">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000135">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000145">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000149">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000156">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000224">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000152">
        <property type="entry name" value="Spectrin, beta, non-erythrocytic 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000159">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000164">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000216">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000206">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000226">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041681">
        <property type="entry name" value="PH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001605">
        <property type="entry name" value="PH_dom-spectrin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15410">
        <property type="entry name" value="PH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00683">
        <property type="entry name" value="SPECTRINPH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0117">Actin capping</keyword>
    <keyword evidence="10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="17">
        <location>
            <begin position="24"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="17">
        <location>
            <begin position="145"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="831"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="16">
        <location>
            <begin position="3747"/>
            <end position="3855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="651"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="3596"/>
            <end position="3645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="3701"/>
            <end position="3746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="3867"/>
            <end position="4059"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="4086"/>
            <end position="4105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="4119"/>
            <end position="4298"/>
        </location>
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            <dbReference type="Proteomes" id="UP001500889"/>
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    <sequence length="4298" mass="492563" checksum="7B9133830032E438" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU11</accession>
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                <person name="Yamamoto D."/>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
    </protein>
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    </gene>
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        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FCH and double SH3 domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000039">
        <property type="entry name" value="FCH and double SH3 domains protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000155">
        <property type="entry name" value="FCH and double SH3 domains protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035460">
        <property type="entry name" value="FCHSD_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF21">
        <property type="entry name" value="PROTEIN NERVOUS WRECK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="12">
        <location>
            <begin position="11"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="545"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="662"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="431"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="727"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="773"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="954"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="497"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="737"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="813"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="845"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="900"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="959"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="991"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1025"/>
            <end position="1039"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF99114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1039" mass="113924" checksum="1263ACF97C974897" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU17</accession>
    <name>A0AAU9FU17_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07422</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FU17"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07844">
        <property type="entry name" value="STKc_PCTAIRE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000131">
        <property type="entry name" value="cyclin-dependent kinase 14 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000007">
        <property type="entry name" value="Cyclin-dependent kinase 14, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF246">
        <property type="entry name" value="ECDYSONE-INDUCED PROTEIN 63E, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="194"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="52"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF99544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="511" mass="58793" checksum="82831A7E4D3FAA83" modified="2024-11-27" version="1">MAELINQQPTKINNNNFGAGVTMREKKGGALQKLKKRLSHSFGRLTISREDGDEATHHHHHHHHHHRGGHGGHHNHGNKVPYNGYNSEEYLDRLEPNGNIPADKTNCRYGDWRSTDSTDHHDRVQRQLSVSSDSKLLDEDIREEMKYHSHVVMRPKKPPRPKSEVFLNKQDPHPRRKRFSAFGGDSPFGKQEAYVKLEPLGEGSYATVYKGFSKLTYQRVALKEIRLQEEEGAPFTAIREASLLKELKHSNIVTLHDIVHTRETLTFVFEYVNTDLSQYMEKHPGGLDHRNVRLFLFQLLRGLSYCHKRRVLHRDVKPQNLLISDCGELKLADFGLARAKSVPSHTYSHEVVTLWYRPPDVLLGSTEYSTSLDMWGVGCIFVEMVTGMPTFPGIRDTYDQLDKIFKLLGTPTEETWTGVTHFPGYKPHKLGFYRPRKLGHNFPRLYDIIEGETIANAFLQLNPEERIGADDALLHPYFAQLPKKLYELPDETSIFTVEGVQLYTEPNRQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5I2</accession>
    <name>A0AAU9G5I2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transient receptor potential cation channel trpm</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_02501</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <location>
            <begin position="1095"/>
            <end position="1106"/>
        </location>
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            <begin position="1121"/>
            <end position="1133"/>
        </location>
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            <end position="1178"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51508"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03760.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1661" mass="186864" checksum="C605905A63E66F45" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5J2</accession>
    <name>A0AAU9G5J2_DROMD</name>
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        <recommendedName>
            <fullName evidence="4">Copper transport protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="6" type="ORF">DMAD_02346</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="BFG02986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF115">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
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        <location>
            <begin position="88"/>
            <end position="111"/>
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    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG02986.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="224" mass="24906" checksum="297C986FA44D9669" modified="2024-11-27" version="1">MDHAHHSAPSVDHSMHHDHAGMHHDHSGMDSSISPIGTTTTTDPTVRDIHAGHHHGGGGGGGGTGTGMEHMMSMAFHFGYNETILFSWWHIDTVAGLIGSMIAIFLLALLYEGLKYYREYLFWKTYNLLEYRPVTGPQRNPEAPRLPSPAAAAPSPVQPSMLSVNHLYQTLLHILQVTLSFLLMLIFMTYNVWLCLMVVLGAAVGYFLFCWKKSVIVDVTEHCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        <name evidence="19" type="ORF">DMAD_02225</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="BFG02825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome A"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:voltage-gated potassium channel activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.260:FF:000003">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000009">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000009">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
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        <property type="entry name" value="K_chnl_volt-dep_EAG"/>
    </dbReference>
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        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
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        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01464">
        <property type="entry name" value="EAGCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    </feature>
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        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
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        <location>
            <begin position="43"/>
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        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="18">
        <location>
            <begin position="113"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="16">
        <location>
            <begin position="594"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="737"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="1071"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1140"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1224"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="758"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="792"/>
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        </location>
    </feature>
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        <location>
            <begin position="820"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="1071"/>
            <end position="1083"/>
        </location>
    </feature>
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        <location>
            <begin position="1084"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1152"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1167"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1235"/>
            <end position="1253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45985">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45985:SF3">
        <property type="entry name" value="CHITIN DEACETYLASE-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043616967" description="Chitin-binding type-2 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="2">
        <location>
            <begin position="27"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG02786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="490" mass="55554" checksum="D143A4C905431C12" modified="2024-11-27" version="1">MQLQHCLVAATLLIVAGVNGQNKEKEEFQCPSHIANGNYADPATCRRFYQCVDGYPYLNRCPSGLYFDDLQKYCTFKDEAKCGPLPTTPAPATDAPADTAQRCSTEDCTLPYCFCSKDGTQIPGDLEAEKIPQIIMLTFDGAVNLNNYDHYTKIFNGKRKNPNGCLIRGTFFLSHEYSNYQQIQHLGYAGHEVATESISQQQGLQDKGYEEWVGEMIGMREILRHFANVSVNDVVGMRAPFLKPGRNTQYKVLEDFGYIYDSSITVPPVAVPVWPYTLDYKISHECKSGTCPSRTFPGVWEVPLNTHYVEGFEGGHCPYMDQCVLHNLDEEEVLAWLQEDFSRYYEQNKAPYMMPFHTNWFQTKPLENGLHKFLDWALELPDVYILTVTQMLQYMTDPKELRDVNTIESWKCDKSIAVAPKPCNIWQTCALSFKIPEQNLTDTRYMETCRECPNVYPWLGDAGGTGVQGRDNYIFSGASGDEAEDDSASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5K0</accession>
    <name>A0AAU9G5K0_DROMD</name>
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        <recommendedName>
            <fullName evidence="3">Centrosomal protein of 162 kDa</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_02317</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CEP162 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G5K0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CEP162-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34031">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 162 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34031:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 162 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="509"/>
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    </feature>
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        <location>
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            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
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            <begin position="158"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="239"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
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            <end position="615"/>
        </location>
    </feature>
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        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
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        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
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            <end position="356"/>
        </location>
    </feature>
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            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="388"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="420"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="445"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG02952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="675" mass="75557" checksum="E5AAE0812B79BCD0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5K1</accession>
    <name>A0AAU9G5K1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Patronin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_02937</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The CKK domain binds microtubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CAMSAP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036449">
        <property type="term" value="C:microtubule minus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030507">
        <property type="term" value="F:spectrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.360:FF:000002">
        <property type="entry name" value="Patronin, isoform M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.360">
        <property type="entry name" value="CKK domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032940">
        <property type="entry name" value="CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031372">
        <property type="entry name" value="CAMSAP_CC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058042">
        <property type="entry name" value="CAMSAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014797">
        <property type="entry name" value="CKK_CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038209">
        <property type="entry name" value="CKK_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595:SF0">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17095">
        <property type="entry name" value="CAMSAP_CC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08683">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25532">
        <property type="entry name" value="CH_CAMSAP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01051">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51508">
        <property type="entry name" value="CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="157"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="CKK" evidence="10">
        <location>
            <begin position="1526"/>
            <end position="1660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="311"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="362"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="526"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="763"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="834"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="889"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="934"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1081"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1135"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1275"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1360"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1411"/>
            <end position="1531"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="641"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="705"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="311"/>
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</entry>
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                <person name="Yamamoto D."/>
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        </source>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
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                <person name="Yamamoto D."/>
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                <person name="Yamamoto D."/>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG02870.1"/>
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        <source>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <name evidence="7" type="ORF">DMAD_02464</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNAP-25 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G601"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031629">
        <property type="term" value="P:synaptic vesicle fusion to presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016082">
        <property type="term" value="P:synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15856">
        <property type="entry name" value="SNARE_SNAP29C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15887">
        <property type="entry name" value="SNARE_SNAP29N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000041">
        <property type="entry name" value="Synaptosomal-associated protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000079">
        <property type="entry name" value="synaptosomal-associated protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF9">
        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="6">
        <location>
            <begin position="197"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="149"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG03136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="262" mass="29417" checksum="40E7B631A0832BAE" modified="2024-11-27" version="1">MANNYLKPVHNYFDDVDRFEDIDDDLFLKNKRTSASSHKPQQRSTNPFEIDDDEDSTASSISAQRQAYAEKRRAIEQRTLDSTEKSLGLLYETEEVGKATAIELAKQREQLEKTSHQLDEINSTLRFSQRHLNGLKSVFGGLKNYLSGNRDQPSSATASPVASQSSQEANNINAGAFGGSSAPLSPAEHYDNHPAGNPFQAHLDSNLEDMCDNLSRLKFLATDLGTEIESQNELLDNMNYKIEDVDLKMTRQNKDMNKLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G604</accession>
    <name>A0AAU9G604_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Trichohyalin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_02316</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G604"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043863241" evidence="2">
        <location>
            <begin position="28"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG02949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="620" mass="74920" checksum="253527F61D2CBBBF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G605</accession>
    <name>A0AAU9G605_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_02659</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G605"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07132">
        <property type="entry name" value="ALDH_F3AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000003">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="469"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG03388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="498" mass="55610" checksum="39D71E6C5C33F6B4" modified="2024-11-27" version="1">MANFDDTLQRARLAFASGKTRNVNFRRKQLENLLRCYEENENDIISALEADLRRPKQESLIVETEFMKNDIKHILYHLSDWVKAEKPSKSIINVMDDVQIYNDPFGVVLVIGAWNYPLQLLLVPVASAIAAGNCVVIKPSEIAGNCAKFIAEVIPKYLDNDCYPVVCGGPSETAELLKQRFDYIFYTGSTRVGKIIHAAANKHLTPTTLELGGKSPCYIDKSVELRTAVKRILWGKLINCGQTCIAPDYILCSKEMQDKFVAEAKDVLKEWYGENIQSSPDLSRVINTNNFQRLIGLMKSGRVAVGGKYDASERYIEPTILVDVKPNDPIMEEEIFGPILPIYNVESAYDAIKFINAREKPLVIYVFSNSNKLVKEFQSNTTSGGFCSNETIMHCGVDVLPFGGVGMSGMGSYHGKYGFDTFTHKKSCLGKDLSAVGEKLASARYPPYSDRKGSFLSFLLRKRRPLPNFYLTHVLAVGLGVGLTVLANYYLQGKLLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G607</accession>
    <name>A0AAU9G607_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KN motif and ankyrin repeat domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_02775</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
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            <taxon>Brachycera</taxon>
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            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6 7">RF1</strain>
        </source>
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        </location>
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        </location>
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    <feature type="repeat" description="ANK" evidence="4">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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            <end position="355"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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            <end position="379"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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            <end position="494"/>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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            <end position="651"/>
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        <location>
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            <end position="771"/>
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        <location>
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        <location>
            <begin position="21"/>
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        <location>
            <begin position="366"/>
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        <location>
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        </location>
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        <source>
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    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03544.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1321" mass="144656" checksum="578DF7E0E27A2F39" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G610</accession>
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        </submittedName>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
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    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-1071">Ligand-gated ion channel</keyword>
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</entry>
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</entry>
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        </source>
    </evidence>
    <sequence length="478" mass="52338" checksum="80928ABAF9F07060" modified="2024-11-27" version="1">MPYNGASNGSGGAGGGGGGATIVVSEGPQNKKIRTGVQQPGENDVHMHARSTQQQNQQQALMNKSNDDLRRKRPETTRPNHILLFTIINPFYPITVDVLHKICHPHGQVLRIVIFKKNGVQAMVEFDNLDAATRARENLNGADIYAGCCTLKIDYAKPEKLNVYKNEPDTSWDYTLSTGEILPKPPLLGPGAAFPPFGAPEYHPTTPDNWKGASIHPTGLMKEPTGVVPGRNAPVAFTPQGQAQGAVMMVYGLDHDTSNTDKLFNLVCLYGNVARIKFLKTKEGTAMVQMGDSVAVERCVQHLNNIPVGTGGKIQIAFSKQNFLSEVINPFLLPDHTPSFKEYTGSKNNRFLSPAQASKNRIQPPSKILHFFNTPPGLTEDQLIGIFNIKDVPATSVRLFPLKTERSSSGLIEFPNISQAVLAIMKCNHLPIEGTKFPFIMKLCFSSSKSMNGAWNNATNEGMIEKENEVDSKVDIYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G618</accession>
    <name>A0AAU9G618_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Matrix metalloproteinase-14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_02668</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="14">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G618"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000022">
        <property type="entry name" value="Matrix metalloproteinase 1, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000007">
        <property type="entry name" value="stromelysin-3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF291">
        <property type="entry name" value="MATRIX METALLOPROTEINASE 1, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00024">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="17">
        <location>
            <begin position="129"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="16">
        <location>
            <begin position="321"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="16">
        <location>
            <begin position="367"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="16">
        <location>
            <begin position="413"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="16">
        <location>
            <begin position="463"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="14">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphotyrosine; by PKDCC" evidence="15">
        <location>
            <position position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFG03403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="573" mass="63474" checksum="0136D0D74036C4E0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFK3</accession>
    <name>A0AAU9GFK3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase C-like 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05038</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </location>
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            <end position="759"/>
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            <begin position="760"/>
            <end position="774"/>
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    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG06571.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0AAU9GFM1</accession>
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            <fullName evidence="9">Protein kinase C-like 3</fullName>
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        <name evidence="7" type="ORF">DMAD_05038</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Neoptera</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <location>
            <begin position="111"/>
            <end position="121"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG06562.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFM6</accession>
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        <submittedName>
            <fullName evidence="13">Protein enabled</fullName>
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    </protein>
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        <name evidence="13" type="ORF">DMAD_05037</name>
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    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
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        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ena/VASP family.</text>
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        <property type="protein sequence ID" value="BFG06552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="protein enabled isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="vasodilator-stimulated phosphoprotein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    <dbReference type="Gene3D" id="1.20.5.1160">
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    </dbReference>
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        <property type="entry name" value="PH-like_dom_sf"/>
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        <property type="entry name" value="Ran_bind_dom"/>
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        <property type="entry name" value="VASP_sf"/>
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        <property type="entry name" value="VASP_tetra"/>
    </dbReference>
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        <property type="entry name" value="WH1/EVH1_dom"/>
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        <property type="entry name" value="PROTEIN ENABLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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    <feature type="domain" description="WH1" evidence="12">
        <location>
            <begin position="153"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="316"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="320"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="381"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="396"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="475"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="497"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="520"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="559"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="611"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="699"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="727"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG06552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="824" mass="88121" checksum="92BB5E554D2DF98D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFM9</accession>
    <name>A0AAU9GFM9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Valine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Valyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_04882</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="2"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GFM9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00817">
        <property type="entry name" value="ValRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000119">
        <property type="entry name" value="Putative valine--tRNA ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.380:FF:000001">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000015">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000020">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000005">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="entry name" value="Valyl-tRNA synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02004">
        <property type="entry name" value="Val_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004349">
        <property type="entry name" value="PRK05729.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF109">
        <property type="entry name" value="VALINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 12 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="14">
        <location>
            <begin position="99"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="15">
        <location>
            <begin position="773"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG06354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1046" mass="118103" checksum="E64B9A273983B8D9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFN0</accession>
    <name>A0AAU9GFN0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase C-like 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05038</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GFN0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20831">
        <property type="entry name" value="C1_dGM13116p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08678">
        <property type="entry name" value="C2_C21orf25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039934">
        <property type="entry name" value="C2CD2/C2CD2L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21119">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21119:SF5">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="5">
        <location>
            <begin position="369"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="6">
        <location>
            <begin position="867"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="70"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="626"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="733"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="641"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="656"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="668"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="733"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="747"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="762"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG06577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="944" mass="102406" checksum="39A1E27F21EC76F2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFN2</accession>
    <name>A0AAU9GFN2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Kinesin-like protein unc-104</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_04956</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for presynaptic maturation, has a role in axonal transport of dense-core vesicles carrying synaptic vesicle precursors, components required for the morphological transformation of axonal growth cones to mature boutons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048490">
        <property type="term" value="P:anterograde synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021700">
        <property type="term" value="P:developmental maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051222">
        <property type="term" value="P:positive regulation of protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040012">
        <property type="term" value="P:regulation of locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051960">
        <property type="term" value="P:regulation of nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010975">
        <property type="term" value="P:regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008582">
        <property type="term" value="P:regulation of synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22705">
        <property type="entry name" value="FHA_KIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01233">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000001">
        <property type="entry name" value="Kinesin family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000004">
        <property type="entry name" value="Kinesin-like protein isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000204">
        <property type="entry name" value="kinesin-like protein unc-104 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
        <property type="entry name" value="Kinesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049780">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF10">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12473">
        <property type="entry name" value="DUF3694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="15">
        <location>
            <begin position="3"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="1542"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="399"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="964"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="593"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="399"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="409"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG06446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1675" mass="189565" checksum="3FFAC0FD39B47071" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFN8</accession>
    <name>A0AAU9GFN8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GIGYF family protein8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_13639</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00072">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051640">
        <property type="entry name" value="GRB10-interact_GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445:SF36">
        <property type="entry name" value="FI03272P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445">
        <property type="entry name" value="GRB10 INTERACTING GYF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00444">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="3">
        <location>
            <begin position="583"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="101"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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</entry>
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    <accession>A0AAU9GFN9</accession>
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        <name evidence="7" type="ORF">DMAD_05038</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <strain evidence="7 8">RF1</strain>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="16">Twitchin</fullName>
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        <name evidence="16" type="ORF">DMAD_13643</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
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            <strain evidence="16 17">RF1</strain>
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    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="8384"/>
            <end position="8402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="8410"/>
            <end position="8422"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="7823"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG06696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="8740" mass="976583" checksum="B1B55B5C5D44A9A7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFQ5</accession>
    <name>A0AAU9GFQ5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dual-specificity kinase</fullName>
            <ecNumber evidence="2">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_13611</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="2"/>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GFQ5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:cytoskeleton"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14224">
        <property type="entry name" value="PKc_DYRK2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000112">
        <property type="entry name" value="Putative dual specificity tyrosine-phosphorylation-regulated kinase 2"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.200.20:FF:000127">
        <property type="entry name" value="Putative dual specificity tyrosine-phosphorylation-regulated kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042521">
        <property type="entry name" value="DYRK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF112">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 3 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="280"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="826"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="832"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG06631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="847" mass="93606" checksum="ED0A0031C0F0B87B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFQ8</accession>
    <name>A0AAU9GFQ8_DROMD</name>
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        <submittedName>
            <fullName evidence="3">Serine-enriched protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_04975</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BFG06470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome E"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051481">
        <property type="entry name" value="BTB-POZ/Galectin-3-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24410">
        <property type="entry name" value="HL07962P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24410:SF46">
        <property type="entry name" value="SERINE-ENRICHED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="40"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="590"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="651"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="712"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="865"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="953"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1086"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1145"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1230"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="368"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="669"/>
            <end position="685"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="727"/>
            <end position="736"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="737"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="765"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="868"/>
            <end position="887"/>
        </location>
    </feature>
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        <location>
            <begin position="1086"/>
            <end position="1095"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1152"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1230"/>
            <end position="1258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG06470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1347" mass="148250" checksum="9EF7C70864ABB51F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFR0</accession>
    <name>A0AAU9GFR0_DROMD</name>
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        <submittedName>
            <fullName evidence="2">Protein unzipped</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_05048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome E"/>
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        <property type="entry name" value="AGAP009604-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN UNZIPPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="434"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG06597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="540" mass="60234" checksum="DBA71FCE8DA2FF27" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFR4</accession>
    <name>A0AAU9GFR4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
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    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Protein CBR-UNC-22"/>
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        <property type="entry name" value="Titin a"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Titin isoform E"/>
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        <property type="entry name" value="titin isoform X1"/>
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        <property type="entry name" value="titin isoform X1"/>
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        <property type="entry name" value="twitchin isoform X2"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
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        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00069">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FNTYPEIII"/>
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    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="36"/>
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        <property type="match status" value="26"/>
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    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="37"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50853">
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        <property type="match status" value="35"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="265"/>
            <end position="302"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="538"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="627"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="669"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
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        </location>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG06491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1129" mass="121511" checksum="5792B5C44B60056D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFR8</accession>
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        <submittedName>
            <fullName evidence="19">Glutamate receptor ionotropic kainate 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_13620</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="1">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="ANF_lig-bd_rcpt"/>
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        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
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        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10 19" id="KW-0675">Receptor</keyword>
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    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="16">
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    <feature type="transmembrane region" description="Helical" evidence="16">
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            <begin position="723"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="909"/>
            <end position="933"/>
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        <location>
            <begin position="435"/>
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    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="18">
        <location>
            <begin position="445"/>
            <end position="509"/>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00008685"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00079"/>
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    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFG06646.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1001" mass="114731" checksum="EB292981A114EFA5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFR9</accession>
    <name>A0AAU9GFR9_DROMD</name>
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        <submittedName>
            <fullName evidence="7">Endophilin-B1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_04984</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the endophilin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9GFR9"/>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07594">
        <property type="entry name" value="BAR_Endophilin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11802">
        <property type="entry name" value="SH3_Endophilin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="endophilin-B2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF76">
        <property type="entry name" value="ENDOPHILIN B, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="6">
        <location>
            <begin position="29"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="315"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="261"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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            <strain evidence="10 11">RF1</strain>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        </source>
    </evidence>
    <sequence length="830" mass="90449" checksum="2BCD044EEE03E849" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GG36</accession>
    <name>A0AAU9GG36_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zyxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_13626</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the zyxin/ajuba family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GG36"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09351">
        <property type="entry name" value="LIM1_LPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09354">
        <property type="entry name" value="LIM2_LPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09437">
        <property type="entry name" value="LIM3_LPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000109">
        <property type="entry name" value="Lipoma preferred partner"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000027">
        <property type="entry name" value="lipoma-preferred partner isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000042">
        <property type="entry name" value="lipoma-preferred partner isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24207">
        <property type="entry name" value="ZYX102 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24207:SF2">
        <property type="entry name" value="ZYX102 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0440">LIM domain</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="13">
        <location>
            <begin position="403"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="13">
        <location>
            <begin position="463"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="13">
        <location>
            <begin position="524"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFG06656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="602" mass="67024" checksum="D7606B8B32841F0C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EQJ7</accession>
    <name>A0AAU9EQJ7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">SH2B adapter protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_07713</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SH2B adapter family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EQJ7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005068">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01231">
        <property type="entry name" value="PH_SH2B_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10346">
        <property type="entry name" value="SH2_SH2B_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000354">
        <property type="entry name" value="Lnk, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000008">
        <property type="entry name" value="SH2B adapter protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015012">
        <property type="entry name" value="Phe_ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036290">
        <property type="entry name" value="Phe_ZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030523">
        <property type="entry name" value="SH2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035057">
        <property type="entry name" value="SH2B1_SH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10872:SF2">
        <property type="entry name" value="LNK, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10872">
        <property type="entry name" value="SH2B ADAPTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08916">
        <property type="entry name" value="Phe_ZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109805">
        <property type="entry name" value="Phenylalanine zipper"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="187"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="6">
        <location>
            <begin position="442"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="547"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="561"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="583"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="593"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF88795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="621" mass="65755" checksum="64E36F7C737A5568" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EQN3</accession>
    <name>A0AAU9EQN3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Isocitrate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_07729</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EQN3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Isocitrate dehydrogenase [NAD] subunit, mitochondrial"/>
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        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
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        <property type="entry name" value="Isocitrate_DH_NAD"/>
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    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
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        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF60">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT, MITOCHONDRIAL"/>
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        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="6">
        <location>
            <begin position="59"/>
            <end position="358"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
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            <dbReference type="ARBA" id="ARBA00022532"/>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="378" mass="41993" checksum="A50EF230D7C75C9F" modified="2024-11-27" version="1">MALRLTQRLLQTQTPFLTRGYPMLVTKEKSEDVAHTKSSLQKKVTRTDIPSAQYGGRHAVTMLPGGGIGPELMTYGNDDLDYAITSIKRNGVALKGNIETKSQELSEVSRNVAIRNELDLYVNVVHCKSYPGIPARHQDIDVVLIRQNTDGEYAMLEHESVPGIVESMKVVTVENAERVARYAFEYARQNNRKKVTTIHKANIMKLSDGLFLECAKRVHKDYPELEHDNMIIDNTCMQAVSKPHQFDVMNMTNLYGTIVSNVLCGLMGGAGLISGRNYGDHYAVFEPGTRNTGTAIAGKNIANPVAMINASIDMLNHLGHKEHANVIYEATYQTIVNDAIRTPDLGGNHSSTDVVENILKILSAKRVNWPHGNYFNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EQQ8</accession>
    <name>A0AAU9EQQ8_DROMD</name>
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        <submittedName>
            <fullName evidence="9">RNA-binding protein with serine-rich domain 1-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_08097</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="BFF89308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="C:ASAP complex"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="RBD_domain_sf"/>
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        <property type="entry name" value="RNPS1_RRM"/>
    </dbReference>
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        <property type="entry name" value="RRM_dom"/>
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        </source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF89308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="373" mass="41724" checksum="F074B82F120B22BF" modified="2024-11-27" version="1">MARAQSPAGEGEKEKDVKEKDTKEKEGKAATSNSSRRERSRKRRGSASSSSDSSRSSSSRSSSGSGSRSSSSSSSDSSSSSSSSSSDSDRSEKNRRRGAAGGGGAAGGKDKDKEKSRTRSRSPRRTTKSPQPSSTKARKEPERERERRSRSRDRARRASSNDRGAVGGDSNNVKRERSRSASRSRSPRRRGRTSGDRTPPPKRRERTRSRTRTRSPTPKPVRIHVGRLTRNVTKDHVFEIFSSFGDVKSVEFPTDRYHPNFGRGMAFVEYGTAEDCESAMKHMDGGQVDGQEITVSPVIIPKQRPPMRRPSPPMRRPQNNRWRSPPQFNRFNNRGGGGRRPSPPNRNRNRSPRRRSRSPIRRRRRSNSSDSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ER21</accession>
    <name>A0AAU9ER21_DROMD</name>
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        <submittedName>
            <fullName evidence="10">Transcription factor capicua</fullName>
        </submittedName>
    </protein>
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        <name evidence="10" type="ORF">DMAD_08180</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BFF89411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Capicua transcriptional repressor b"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HMG-BOX TRANSCRIPTION FACTOR BBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13059:SF13">
        <property type="entry name" value="PROTEIN CAPICUA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16090">
        <property type="entry name" value="DUF4819"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="9">
        <location>
            <begin position="1309"/>
            <end position="1377"/>
        </location>
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        </location>
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        <location>
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        </location>
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    </feature>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRAMP family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EZA0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00221">
        <property type="entry name" value="NRAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01197">
        <property type="entry name" value="nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037982">
        <property type="entry name" value="Nramp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF33">
        <property type="entry name" value="NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="304"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="378"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="469"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="495"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="525"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF90324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="610" mass="67313" checksum="3AEAB072905D157A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZA1</accession>
    <name>A0AAU9EZA1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_07900</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the p23/wos2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EZA1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06465">
        <property type="entry name" value="p23_hB-ind1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000013">
        <property type="entry name" value="Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045250">
        <property type="entry name" value="p23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932:SF1">
        <property type="entry name" value="CO-CHAPERONE PROTEIN DAF-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932">
        <property type="entry name" value="TELOMERASE-BINDING PROTEIN P23 HSP90 CO-CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="3">
        <location>
            <begin position="7"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="115"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF89061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="183" mass="20346" checksum="A563552BF8E29E9F" modified="2024-11-27" version="1">MSAAAGSIPPPVSWAQRSDLVYVIIDVECKDIEQKVTENSFTFKGVNALDASKKYEVTLNFFGTVDPEKVTSKNIGRCLEFTIPKKASGPFWPSLTTDKTKLHFLKANFAKWRDESDDEEGDAKDNGMFGNFLSNSGGDWNNKFDDFNVDDEEEDSDDNIPSLSQNDEEEEEAGEGDKKKPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZB3</accession>
    <name>A0AAU9EZB3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Cadherin-87A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_07911</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000443">
        <property type="entry name" value="AGAP003579-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000266">
        <property type="entry name" value="Cadherin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000306">
        <property type="entry name" value="Cadherin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000394">
        <property type="entry name" value="Cadherin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000098">
        <property type="entry name" value="cadherin-23 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000378">
        <property type="entry name" value="Cadherin-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000124">
        <property type="entry name" value="Cadherin-related family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000168">
        <property type="entry name" value="Cadherin-related family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000445">
        <property type="entry name" value="GG17179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000118">
        <property type="entry name" value="protocadherin Fat 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF438">
        <property type="entry name" value="CADHERIN 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
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    <sequence length="1982" mass="218549" checksum="53282C005D894633" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="2">dual-specificity kinase</fullName>
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        <name evidence="18" type="ORF">DMAD_09329</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="18 19">RF1</strain>
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            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
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        <reaction evidence="14">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
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        <subcellularLocation>
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        <text evidence="11">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.</text>
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        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFF90906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1277" mass="142421" checksum="9B1B93A9ECC2438B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F2P4</accession>
    <name>A0AAU9F2P4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nose resistant to fluoxetine protein 6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_10120</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F2P4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006621">
        <property type="entry name" value="Nose-resist-to-fluoxetine_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052728">
        <property type="entry name" value="O2_lipid_transport_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161:SF0">
        <property type="entry name" value="O-ACYLTRANSFERASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20146">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00703">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044713088" evidence="2">
        <location>
            <begin position="20"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="485"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="518"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="562"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="633"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="Nose resistant-to-fluoxetine protein N-terminal" evidence="3">
        <location>
            <begin position="67"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF91964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="714" mass="80207" checksum="5D35B95C084922A3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F307</accession>
    <name>A0AAU9F307_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Laccase-11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_07709</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F307"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13858">
        <property type="entry name" value="CuRO_1_tcLCC2_insect_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13884">
        <property type="entry name" value="CuRO_2_tcLCC_insect_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13905">
        <property type="entry name" value="CuRO_3_tcLLC2_insect_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000045">
        <property type="entry name" value="Laccase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF394">
        <property type="entry name" value="FI03373P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043695230" evidence="5">
        <location>
            <begin position="29"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="8">
        <location>
            <begin position="136"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="279"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="7">
        <location>
            <begin position="547"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF88790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="690" mass="78367" checksum="FEFF9BF7AEF7C5D0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F317</accession>
    <name>A0AAU9F317_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein roadkill</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_09034</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F317"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18520">
        <property type="entry name" value="BACK_roadkill_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18345">
        <property type="entry name" value="BTB_POZ_roadkill-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000028">
        <property type="entry name" value="Speckle-type POZ protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000008">
        <property type="entry name" value="Speckle-type POZ protein-like a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056423">
        <property type="entry name" value="BACK_BPM_SPOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24413">
        <property type="entry name" value="SPECKLE-TYPE POZ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24570">
        <property type="entry name" value="BACK_BPM_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="MATH" evidence="7">
        <location>
            <begin position="63"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="6">
        <location>
            <begin position="232"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF90529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="406" mass="45759" checksum="B410288C590C219E" modified="2024-11-27" version="1">MDLIHEPRLPVNECQASQTARVTSNLHASSSTMAVSRVPSPPLPEVNTPVAENWCYTQVKVVKFSYMWTINNFSFCREEMGEVLKSSTFSAGANDKLKWCLRVNPKGLDEESKDYLSLYLLLVSCNKSEVRAKFKFSILNAKREETKAMESQRAYRFVQGKDWGFKKFIRRDFLLDEANGLLPEDKLTIFCEVSVVADSVNISGQSNIVQFKVPECKLSEDLGNLFDNEKFSDVTLSVGGREFQAHKAILAARSDVFAAMFEHEMEERKLNRVAITDVDHEVLKEMLRFIYTGKAPNLEKMADDLLAAADKYALEKLKVMCEEALCVNLSVETAAETLILADLHSADQLKAQTIDFINTHATDVMETSGWQNMITTHSHLIAEAFRALATQQIPPIGPPRKRVKMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F319</accession>
    <name>A0AAU9F319_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Low-density lipoprotein receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_07726</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F319"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000105">
        <property type="entry name" value="Lipophorin receptor 1, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000002">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000015">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000034">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000008">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000113">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000030">
        <property type="entry name" value="Sortilin related receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF14">
        <property type="entry name" value="MEGALIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="23" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="15 23" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="985"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="275"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like calcium-binding" evidence="21">
        <location>
            <begin position="525"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="528"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like calcium-binding" evidence="21">
        <location>
            <begin position="566"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="569"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="18">
        <location>
            <begin position="652"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="18">
        <location>
            <begin position="696"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="18">
        <location>
            <begin position="739"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="18">
        <location>
            <begin position="783"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="878"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="930"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="938"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="950"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="297"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="316"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="335"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="356"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="444"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="463"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="495"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF88820.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1070" mass="118401" checksum="0EF3807C4D4419AE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">RF1</strain>
        </source>
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        <property type="protein sequence ID" value="BFF91206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <location>
            <begin position="1"/>
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        </location>
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            <begin position="81"/>
            <end position="137"/>
        </location>
    </feature>
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        <location>
            <begin position="152"/>
            <end position="206"/>
        </location>
    </feature>
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            <begin position="236"/>
            <end position="271"/>
        </location>
    </feature>
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            <end position="112"/>
        </location>
    </feature>
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            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF91206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="463" mass="44397" checksum="5B921FDB70DE1673" modified="2024-11-27" version="1">MKFIVCLLLVVALVGQQQTSARSVSSSLHQFDNELGQAASEFGHELGDEGTQFGHEASNSAINFGHQVSQEAEQVGSAEIGSLEKQAQSSSSSSSSSSSADSTSSKNSATDKVVVSTDQSTNKVSSSKTTTCTCVDSVDNSGKDASGAAKSVAKDASGAAKTVGKDASGAAKSVAKDATGAAKSVAKDATGAAKDATGDAKSVAKDATGAAKSVAKDATGAAKSVAKDATGAAKSVAKDATGAAKDATGNAKSVAKDATGAAKDATGDAKSVVKDATGAAKSVAKDATGAAKSVAKDATGAAKSVAKDATGGAKSVAKDATNGADTIAKDAVNGADTIANNAANAADTIANNAADAADTIANNAANAAGTIANNAVNGSDTIANNAANAAGTIANNAANGADTIAKDAVNGADTIAKDAVNGADTIANNAANAADTIANNAADAADTIANNASNFAHSIASIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCP1</accession>
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        <submittedName>
            <fullName evidence="7">Breast cancer anti-estrogen resistance protein 3 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_11315</name>
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    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transcription Factor, Ets-1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SH2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SH2_SHEP1/BCAR3/NSP1"/>
    </dbReference>
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        <property type="entry name" value="BREAST CANCER ANTI-ESTROGEN RESISTANCE PROTEIN 3 HOMOLOG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <feature type="domain" description="SH2" evidence="5">
        <location>
            <begin position="242"/>
            <end position="341"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
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    </feature>
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            <end position="523"/>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF93459.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="967" mass="107563" checksum="BD038BB9CBAEDEF0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCP7</accession>
    <name>A0AAU9FCP7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_11317</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the tetraspanin (TM4SF) family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
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        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF431">
        <property type="entry name" value="TETRASPANIN 26A, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="259"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
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            <begin position="159"/>
            <end position="175"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF93463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="295" mass="33502" checksum="818E7E262FDB773A" modified="2024-11-27" version="1">MPAVRKYRRETSEISCCLKYLLFTSNVFIFVAALMVLSVGIWAWSEKDMFRNIAKLSFIALDPAFVLIILGGITFLLGFMGSVGALRENTCLLAAYAIFLSILLIAEIGFCALAFVLKDKGWIKDQATEGLKAFIRHYREDADQQNLIDWIQEDWLQCCGIDGPKDWDSNNYFNCSSIAIGSREACGVPFSCCRRRPQELIKNKQCGYDVRKEGYVSGFRFRFTRNHNPIINLCKQPVDRNIHERGCLRAGEDWLEAHLISVAAACVGLLMLQILGICFAQNLRADIYTQKSKWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCQ1</accession>
    <name>A0AAU9FCQ1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Krueppel homolog 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_11288</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FCQ1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001855">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001399">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000072">
        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF371">
        <property type="entry name" value="ZINC FINGER PROTEIN 112-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="303"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="331"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="389"/>
            <end position="416"/>
        </location>
    </feature>
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        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="421"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="491"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="705"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="752"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="422"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="491"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="533"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="707"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="755"/>
            <end position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF93430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="804" mass="86653" checksum="F0EA93B723AC51F3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCQ2</accession>
    <name>A0AAU9FCQ2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">[histone H3]-trimethyl-L-lysine(4) demethylase</fullName>
            <ecNumber evidence="4">1.14.11.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_11318</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 2-oxoglutarate + 3 O2 = L-lysyl(4)-[histone H3] + 3 formaldehyde + 3 succinate + 3 CO2</text>
            <dbReference type="Rhea" id="RHEA:60208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
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    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:histone H3K4me/H3K4me2/H3K4me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lysine (K)-specific demethylase 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000028">
        <property type="entry name" value="Lysine-specific demethylase lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000001">
        <property type="entry name" value="Putative lysine-specific demethylase 5b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="ARID_dom"/>
    </dbReference>
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        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048615">
        <property type="entry name" value="KDM5_C-hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013637">
        <property type="entry name" value="Lys_sp_deMease-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004198">
        <property type="entry name" value="Znf_C5HC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF33">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21323">
        <property type="entry name" value="KDM5_C-hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08429">
        <property type="entry name" value="PLU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02928">
        <property type="entry name" value="zf-C5HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZF_PHD_1"/>
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        <property type="entry name" value="ZF_PHD_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="21">
        <location>
            <begin position="154"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="ARID" evidence="20">
        <location>
            <begin position="219"/>
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            <begin position="449"/>
            <end position="499"/>
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        <location>
            <begin position="592"/>
            <end position="758"/>
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        <location>
            <begin position="1289"/>
            <end position="1358"/>
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        <location>
            <begin position="1808"/>
            <end position="1863"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1417"/>
            <end position="1501"/>
        </location>
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        </location>
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        <location>
            <begin position="1869"/>
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    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="15"/>
            <end position="29"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="30"/>
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            <begin position="47"/>
            <end position="59"/>
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            <begin position="129"/>
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            <begin position="1427"/>
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            <begin position="1462"/>
            <end position="1472"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1876"/>
            <end position="1887"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
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    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BFF93465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1899" mass="208785" checksum="97BB7C8FA955019C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCQ7</accession>
    <name>A0AAU9FCQ7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cyclin-dependent kinase-like 2</fullName>
            <ecNumber evidence="4">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_11364</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="4"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FCQ7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07847">
        <property type="entry name" value="STKc_CDKL1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000049">
        <property type="entry name" value="cyclin-dependent kinase-like 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000261">
        <property type="entry name" value="cyclin-dependent kinase-like 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF222">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="24"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="364"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="368"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFF93521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="412" mass="47370" checksum="BE7515117C7DF147" modified="2024-11-27" version="1">MKMFEISKLFSLRRPQGSSKMDRYEKLSRLGEGSYGVVYKCRDRETGALVAVKRFVESEDDPAIRKIALREIRLLKNLKHPNLVSLLEVFRRKRRLHLVFEFCELTVLHELERHPQGCPEHLTKQICYQTLLGVAYCHKQGCLHRDIKPENILLTAQGQVKLCDFGFARMLSPGENYTDYVATRWYRAPELLVGDTQYGTPVDVWAIGCLFAELVRGEALWPGRSDVDQLYLIRKTLGDLLPRHIQIFGQNEYFKGITLPVPPTLEPLEDKMPPKSLQNPLTIDFLKKCLDKDPVKRWSCEKLIKHSYFDDYIAKQREQEHVNSMEAATLRQQQLASQQFMLATAAQQLQTGAAQAAAIAATRDKSKTSNTSLPLLTGSQHHHHQHQDYMKLQPLNKNGNLLHRTEHHLPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCR4</accession>
    <name>A0AAU9FCR4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extensin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_11325</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
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        <location>
            <begin position="23"/>
            <end position="447"/>
        </location>
    </feature>
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            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="218"/>
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            <begin position="218"/>
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            <end position="276"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF93474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="447" mass="47355" checksum="32468DBA3AFED01F" modified="2024-11-27" version="1">MMSRSLTVILVLLCCTLAICQAIQELRDVPTNRIAAPGEAQYYLMADAGSSVGPNDALKRTNRSLLKWWDDLFRQNNCNSCNNNYNNNNNAVYPAVAPVQPQTNCYGNGLQLQDLDPFKQMKLFKKMPDLFGNPLGNCGGLCGGNCGQQPQVNYLAPSPTPGYGGDDGYAKPNAEYNGPGDGDAGYVAPVVAPAGSYDAPAVPPASYEAPAPSYEAPAPNYEAPAPSYEAPEAPPSSYEAPAPVYDAPAPPAASYEQPAATPDSYSKVSDYSPPASSYNAAQPPQIVYQPIIYVSAPPASRTEVATKVQVDEQSYNTPTPPAPPVYEVPTPVMPPSYAAPPSSATPCGSYLPIWGVPVYNYGTQQAAPPPAPAYAPAPPIYAPAPVAPQAYAPPPVYAAPAPQSPGYATPSCETPIRLSLIDQPYRVAPELFEEYNYRLGLAAQNRL</sequence>
</entry>
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            <fullName evidence="7">Tetraspanin</fullName>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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            <strain evidence="8 9">RF1</strain>
        </source>
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        <subcellularLocation>
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    </dbReference>
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        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
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        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF431">
        <property type="entry name" value="TETRASPANIN 26A, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TMFOUR"/>
    </dbReference>
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        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="117"/>
        </location>
    </feature>
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        <location>
            <begin position="240"/>
            <end position="264"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="276" mass="31387" checksum="A0612E41AE95B459" modified="2024-11-27" version="1">MPAVRKYRRETSEISCCLKYLLFTSNVFIFVAALMVLSVGIWAWSEKDMFRNIAKLSFIALDPAFVLIILGGITFLLGFMGSVGALRENTCLLAAYAIFLSILLIAEIGFCALAFVLKDKGWIKDQATEGLKAFIRHYREDADQQNLIDWIQEDWLQCCGIDGPKDWDSNNYFNCSSIAIGSREACGVPFSCCRRRPQELIKNKQCGYDVRKEGYAMELSKIIYEKGCVQAGEEWMEHNLIIISTAVIVVMFFQILGICFAQNLRADIYTQKSKWH</sequence>
</entry>
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    <accession>A0AAU9FCS9</accession>
    <name>A0AAU9FCS9_DROMD</name>
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        <submittedName>
            <fullName evidence="7">Serine/threonine-protein kinase 11-interacting protein</fullName>
        </submittedName>
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        <name evidence="7" type="ORF">DMAD_11330</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
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        <property type="entry name" value="LIP1_N"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF69">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 11-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LIP1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="LKB1 serine/threonine kinase interacting protein 1 N-terminal" evidence="6">
        <location>
            <begin position="2"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="LKB1 serine/threonine kinase interacting protein 1 N-terminal" evidence="6">
        <location>
            <begin position="70"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="364"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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        </location>
    </feature>
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        </location>
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        </location>
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        <location>
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        </location>
    </feature>
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            <end position="408"/>
        </location>
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            <end position="428"/>
        </location>
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        <location>
            <begin position="842"/>
            <end position="861"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF15904"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1308" mass="146479" checksum="95AE50243169B73C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FD19</accession>
    <name>A0AAU9FD19_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_11388</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EEIG family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome U"/>
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        <location>
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            <end position="154"/>
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            <begin position="333"/>
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            <begin position="498"/>
            <end position="520"/>
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        <location>
            <begin position="786"/>
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        <location>
            <begin position="341"/>
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        </location>
    </feature>
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        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="793"/>
            <end position="821"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF93550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="886" mass="96950" checksum="350A4E2BEB28F64D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FD26</accession>
    <name>A0AAU9FD26_DROMD</name>
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        <recommendedName>
            <fullName evidence="3">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">DMAD_11388</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the EEIG family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="12"/>
            <end position="154"/>
        </location>
    </feature>
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        <location>
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            <end position="368"/>
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        </location>
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            <end position="812"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00034780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="BFF93552.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1094" mass="117408" checksum="9D5AE3EB0A376DE7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">DMAD_11385</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
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            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="128"/>
        </location>
    </feature>
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            <end position="297"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="478"/>
        </location>
    </feature>
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        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF93545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="671" mass="74879" checksum="DBE2A8E5270CE2D2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FD35</accession>
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        <submittedName>
            <fullName evidence="16">Potassium voltage-gated channel protein Shaw</fullName>
        </submittedName>
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        <name evidence="16" type="ORF">DMAD_11481</name>
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    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Shaw sub-subfamily.</text>
    </comment>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0032590">
        <property type="term" value="C:dendrite membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032809">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030431">
        <property type="term" value="P:sleep"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
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        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003974">
        <property type="entry name" value="K_chnl_volt-dep_Kv3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="SHAW-LIKE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01498">
        <property type="entry name" value="SHAWCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="15">
        <location>
            <begin position="5"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="205"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="509"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="803"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="224"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="549"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="810"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF93671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="830" mass="90765" checksum="C03496C319099175" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FD38</accession>
    <name>A0AAU9FD38_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dynein axonemal assembly factor 1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Defective transmitter-recycling protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Leucine-rich repeat-containing protein 50 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_11394</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cilium-specific protein required for cilia structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNAAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000166">
        <property type="entry name" value="Dynein assembly factor 1, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000331">
        <property type="entry name" value="Dynein assembly factor 1, axonemal homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF9">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="310"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="527"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1061"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1297"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1497"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1602"/>
            <end position="1624"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="727"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="845"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1209"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1437"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="312"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="336"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1078"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1506"/>
            <end position="1517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1609"/>
            <end position="1624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF93561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1686" mass="190623" checksum="3248FBC8FC6E6930" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FD39</accession>
    <name>A0AAU9FD39_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_11413</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FD39"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22666">
        <property type="entry name" value="FHA_CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14084">
        <property type="entry name" value="STKc_Chk2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000070">
        <property type="entry name" value="Ovarian-specific serine/threonine-protein kinase Lok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001010">
        <property type="entry name" value="Serine/threonine-protein kinase Chk2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="13">
        <location>
            <begin position="26"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="131"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF93583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="436" mass="49903" checksum="84E53AE8A5AA1084" modified="2024-11-27" version="1">MERIVWGRLYGKNIKIKSLDLNNDEFTAGRGEANDLILTLNDLPEKILTRISKVHFIIKRANCELTNPVYIQDLSRNGTFVNNEKIGTNKMRILKNDDVISLAHPTYKAFVFKDLSPNEAIGLPEEITKTYYVNRKLGSGAYGLVRLVYETRTCEQFAMKIVKKNMLAGSAHPTTNLSDPDRVLNEAKIMKNLAHPCVVRMHDIVDKPDSVYMVLEFMRGGDLLNRIISKKLLSEETSKLYFYQMCHAVKYLHDRGITHRDLKPDNVLLESGDEDTLLKVSDFGLSKFVQKDSVMRTLCGTPLYVAPEVLITGGREAYTRKVDIWSLGVVLFTCLSGTLPFSDEYGSPVAEQIKKAKFAYRHPAWKGISQRAKLLINQMLIADPQKRPSIDDVLQANWMRDTQMLQKAKRLMKLDRMEIEEEENNFLEPPTKRSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FD42</accession>
    <name>A0AAU9FD42_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Angiotensin-converting enzyme</fullName>
            <ecNumber evidence="13">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_11451</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12 13">Belongs to the peptidase M2 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="13"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FD42"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008241">
        <property type="term" value="F:peptidyl-dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06461">
        <property type="entry name" value="M2_ACE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001548">
        <property type="entry name" value="Peptidase_M2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10514">
        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10514:SF44">
        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01401">
        <property type="entry name" value="Peptidase_M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00791">
        <property type="entry name" value="PEPDIPTASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52011">
        <property type="entry name" value="PEPTIDASE_M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3 10 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6 13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 14" id="KW-0732">Signal</keyword>
    <keyword evidence="9 13" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043471060" description="Angiotensin-converting enzyme" evidence="14">
        <location>
            <begin position="25"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor 1" evidence="5">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor 2" evidence="7">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor 1" evidence="5">
        <location>
            <position position="507"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor 2" evidence="7">
        <location>
            <position position="507"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="516"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="6">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; partial" evidence="6">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; partial" evidence="6">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="144"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10 12">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10 12">
        <location>
            <begin position="532"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-10"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-11"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF93634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="632" mass="73317" checksum="0766C30DCECD3362" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLA0</accession>
    <name>A0AAU9FLA0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Rab11 family-interacting protein 4B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_05193</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Recycling endosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLA0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NUCLEAR FALLOUT, ISOFORM J"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="458"/>
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        </location>
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        <location>
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            <begin position="237"/>
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            <begin position="33"/>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="78"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004654"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51511"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF96587.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="525" mass="59765" checksum="DED4D446073306DC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLA1</accession>
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        <submittedName>
            <fullName evidence="7">Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1</fullName>
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        <name evidence="7" type="ORF">DMAD_05071</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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        <text evidence="2">Belongs to the glycosyltransferase 31 family. Beta3-Gal-T subfamily.</text>
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        <property type="protein sequence ID" value="BFF96405.1"/>
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        </source>
    </evidence>
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        </source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF96405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLA2</accession>
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        <submittedName>
            <fullName evidence="2">Mediator of RNA polymerase II transcription subunit 12</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">DMAD_13552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BFF96331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="179"/>
        </location>
    </feature>
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        <location>
            <begin position="210"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="545"/>
            <end position="597"/>
        </location>
    </feature>
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        <location>
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            <end position="739"/>
        </location>
    </feature>
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        <location>
            <begin position="775"/>
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        </location>
    </feature>
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        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
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        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="497"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="574"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="662"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="682"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF96331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="930" mass="108102" checksum="C0436755C24D4B5F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLB3</accession>
    <name>A0AAU9FLB3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Formin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05202</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLB3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014767">
        <property type="entry name" value="DAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043592">
        <property type="entry name" value="FMNL_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857">
        <property type="entry name" value="FORMIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857:SF4">
        <property type="entry name" value="FORMIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="CYP24A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
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        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF120">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EP450I"/>
    </dbReference>
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        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0349">Heme</keyword>
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    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043784512" evidence="12">
        <location>
            <begin position="17"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF96563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="521" mass="59312" checksum="BF6612073F25964A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLC3</accession>
    <name>A0AAU9FLC3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BAG family molecular chaperone regulator 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_05091</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLC3"/>
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        <property type="component" value="Chromosome J"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="13"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF96445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="240" mass="27542" checksum="C5A1B3B39A606AA9" modified="2024-11-27" version="1">MLDNLRSIFRKRRHCSEETPPPSEDPKILQSCEDDEGRPGCNDDYSVAWPDAYFNSTPADRTINTNERFVNILDQIDARVEKLRKDALNLQEMKDYLLMSMDLIKSNEMLQKMRESEREEVMLYIQRVSSRLGTVELNVRTVRDNSQEDSLSQINSLIDTMIKVGDPVIARQRCQLYLNACCTSAMDPSGHLDTLYEADVGPIDKKFESALLGCTLDDQKNIKKRLQALMGYLNKQTVSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLC4</accession>
    <name>A0AAU9FLC4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein bunched class 1/class 3/D/E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_13581</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TSC-22/Dip/Bun family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF96366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLC4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21936">
        <property type="entry name" value="ZIP_TSC22D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.490:FF:000002">
        <property type="entry name" value="TSC22 domain family, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.490">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000580">
        <property type="entry name" value="TSC22/Bun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047862">
        <property type="entry name" value="TSC22/BUN_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46745">
        <property type="entry name" value="TSC22 DOMAIN FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46745:SF1">
        <property type="entry name" value="TSC22 DOMAIN FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01166">
        <property type="entry name" value="TSC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58026">
        <property type="entry name" value="Delta-sleep-inducing peptide immunoreactive peptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01289">
        <property type="entry name" value="TSC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="79"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF96366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="227" mass="23410" checksum="A83604179BDD4401" modified="2024-11-27" version="1">MLKYPRTSSSTSSGYFDDDEAMMNSYQTPPASPQSVPQYLQRHMVRMFSTEVDNASGTSAVAIDNKIEQAMDLVKSHLMIAVREEVEVLKERISELMDKINKLELENNILKSNIPQETLQQLQMQLQIAGTQQPAAVGAPPATPAIQAPPAVVAGAGQAAVVAAVGAAATSPATAAAPTTIPNGSAENGGSVIVDAVAAVEQQAAAAAAAAASVQTATPVNTNGPMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLC6</accession>
    <name>A0AAU9FLC6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Down syndrome cell adhesion molecule-like protein Dscam2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_05310</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
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        <location>
            <begin position="1286"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="216"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="228"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF96634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1309" mass="140661" checksum="7EE3C379F1733CD4" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLD1</accession>
    <name>A0AAU9FLD1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">COMM domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DMAD_05179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLD1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047155">
        <property type="entry name" value="COMMD4/6/7/8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231:SF4">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 4-8 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21672">
        <property type="entry name" value="COMM_HN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF96568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="196" mass="21673" checksum="969A277F12AAE437" modified="2024-11-27" version="1">MKFRFCGEGDCPDWVLAEIISTLSNLSIENLEKLSDLVATGICGGTFEESGIKLLTSTLTNEGKTAVACIHFMLINAARYNCTESIFGEEIQQLGLPKEHAAAMCRVLQKHSGCIRQKLIDKAFRINELQSVSNVTSSGETPANCATLELKISQELVEGLPKDTTQIVNLERSQMIALLEELKVCRDIMDKYENKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTM0</accession>
    <name>A0AAU9FTM0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">phospholipase A2</fullName>
            <ecNumber evidence="1">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_06873</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:15802"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="1"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTM0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047499">
        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052816">
        <property type="term" value="F:long-chain fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000304">
        <property type="term" value="P:positive regulation of ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07212">
        <property type="entry name" value="Pat_PNPLA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000006">
        <property type="entry name" value="85/88 kDa calcium-independent phospholipase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047148">
        <property type="entry name" value="PLPL9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24139:SF34">
        <property type="entry name" value="85_88 KDA CALCIUM-INDEPENDENT PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24139">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="217"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="311"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="344"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="10">
        <location>
            <begin position="553"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="513"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="8">
        <location>
            <begin position="557"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="8">
        <location>
            <begin position="589"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="8">
        <location>
            <begin position="724"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="524"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="591"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF98819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="882" mass="97238" checksum="D5422A7C90458331" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTM2</accession>
    <name>A0AAU9FTM2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">arginine kinase</fullName>
            <ecNumber evidence="1">2.7.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_07101</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.3" evidence="1"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTM2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004054">
        <property type="term" value="F:arginine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004111">
        <property type="term" value="F:creatine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046314">
        <property type="term" value="P:phosphocreatine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000006">
        <property type="entry name" value="Arginine kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000749">
        <property type="entry name" value="ATP-guanido_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022415">
        <property type="entry name" value="ATP-guanido_PTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022414">
        <property type="entry name" value="ATP-guanido_PTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547:SF38">
        <property type="entry name" value="ARGININE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547">
        <property type="entry name" value="ARGININE OR CREATINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00217">
        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00112">
        <property type="entry name" value="PHOSPHAGEN_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51510">
        <property type="entry name" value="PHOSPHAGEN_KINASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    </protein>
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                <person name="Llopart A."/>
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</entry>
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            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 24 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTS3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021429">
        <property type="entry name" value="Mediator_Med24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12898">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12898:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11277">
        <property type="entry name" value="Med24_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043560741" description="Mediator of RNA polymerase II transcription subunit 24" evidence="10">
        <location>
            <begin position="24"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="803"/>
            <end position="823"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF98928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="975" mass="109320" checksum="845465238913A9F6" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTS5</accession>
    <name>A0AAU9FTS5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Titin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_07040</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Myofibril</location>
            <location evidence="2">Sarcomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Ig"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="CDD" id="cd11856">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Muscle M-line assembly protein unc-89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000147">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myosin, light chain kinase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000119">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000430">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Sallimus, isoform P"/>
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    </dbReference>
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        <property type="entry name" value="Sallimus, isoform P"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:000474">
        <property type="entry name" value="Sallimus, isoform Q"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:001154">
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Sallimus, isoform Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000050">
        <property type="entry name" value="Titin isoform B"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000962">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="twitchin isoform X4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="47"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_I-set"/>
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    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
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        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633:SF4">
        <property type="entry name" value="MYOPALLADIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="42"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="42"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="38"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="42"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="40"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="93"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="264"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="381"/>
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        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="480"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="627"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="760"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="900"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="1034"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="1168"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="1301"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="1434"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="1568"/>
            <end position="1653"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="1701"/>
            <end position="1791"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="1834"/>
            <end position="1927"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
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        </location>
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        </location>
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        <location>
            <begin position="13839"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="14209"/>
            <end position="14221"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004204"/>
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    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
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        </source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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        <source>
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        <source>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF99039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="16413" mass="1869931" checksum="EE20D3606E8798D7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTS8</accession>
    <name>A0AAU9FTS8_DROMD</name>
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        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_06918</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.</text>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 61F"/>
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        <property type="entry name" value="Y_phosphatase"/>
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    </dbReference>
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        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
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        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="41"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="10">
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            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="406"/>
            <end position="564"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="423"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="499"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="522"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="546"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF98879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="564" mass="63818" checksum="E019F4A07958F9F1" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTT3</accession>
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        <recommendedName>
            <fullName evidence="17">PH-sensitive chloride channel 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_07374</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTT3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099095">
        <property type="term" value="F:ligand-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="LGIC_ECD_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19049">
        <property type="entry name" value="LGIC_TM_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="glutamate-gated chloride channel isoform X12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000020">
        <property type="entry name" value="glutamate-gated chloride channel isoform X12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
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        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
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            <end position="975"/>
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            <begin position="401"/>
            <end position="594"/>
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            <end position="971"/>
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        </location>
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        </location>
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            <begin position="777"/>
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        </location>
    </feature>
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        </location>
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        </location>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF99476.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="979" mass="108644" checksum="C2215F380C6536FE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTT8</accession>
    <name>A0AAU9FTT8_DROMD</name>
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        <recommendedName>
            <fullName evidence="2">MADF domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="ORF">DMAD_06910</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
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        </location>
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        </source>
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            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF98871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="299" mass="34419" checksum="20FC4B3BBDB0E030" modified="2024-11-27" version="1">MTASLPDRFSAGKRQFWREFLALYQDMPQLWDANHVNYRNKELRNRAYERLETKLREIQPNATRTEVGRRINIFRTNYRREQMRILKQKELGFQSDLCKPTLWFYDYMGFLLTQESFQQGRSRKVRGSATIKPKRMFEFGKDASSDPLDTVSEWATKQTIASYLPEPDAPPSEENSLLSPKDEIIEQDGEPMDTDRHPPDDDVKPETGDLNTYDYTDRTNVSTSAALTVGRQSNGLDAHPALSEASEVLAKSWAVQYEEMAPTQRILARKAIADILFEGCMGNLRINRGEQRSSVGNHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAU9FTT9_DROMD</name>
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        <submittedName>
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    </protein>
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    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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            <end position="378"/>
        </location>
    </feature>
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        <location>
            <begin position="399"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="422"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="466"/>
            <end position="487"/>
        </location>
    </feature>
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        <location>
            <begin position="80"/>
            <end position="483"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF99054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="493" mass="54163" checksum="0250903C0C00166F" modified="2024-11-27" version="1">MVTVKIPTVVSSGYDSEKTQGGRNNASIGQPKFIRSDMADVPVQQAAGSTLPLVITRKKGGGDSDDGNYNPFEHRKVEHPTSDLETFVHLLKGSLGSGILAMPMAFSHAGLWFGLVATFAVGTLCTYCVHVLVKCAHILCRRRKIPMMGFADVAEQAFLDGPPALNRWSRFIRFMVNTFLVLDLLGCCCIYLVFVATNVEQVVSVYLETVLSVRVWIMIVTVPLIFMCLVRNLKFLTPFSMIANILMFVGIVITFIYMFTDLPAPSERPGLVAVSEWPLFFGTVIFALEGIGVVMSLENDMRNPTHFIGCPSVLNFGMGLVIALYTLVGFFGFLKYGPDTEASITLNLPVEDKLAQSVKLMIAIAIFFTFTLQFYVPVSILWKGLEHKIRPERQNISEYGLRVALVVLCGGIAVALPNLGPFISLIGAVCLSTLGMIVPATIELAVYHEDPGYGRFNWRLWKNSGLILFGVVGFVAGTYVSIIEFHAEFSGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTU3</accession>
    <name>A0AAU9FTU3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_07166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTU3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005373">
        <property type="entry name" value="PHAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039156">
        <property type="entry name" value="PHAF1/BROMI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465:SF2">
        <property type="entry name" value="PHAGOSOME ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465">
        <property type="entry name" value="UPF0183 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03676">
        <property type="entry name" value="PHAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF99208.1"/>
        </source>
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    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="244" mass="28057" checksum="40D97907BD17A32B" modified="2024-11-27" version="1">MYLESATVLRSSYGHTKGLKLNLFTEGSGRALEPRRQCFTRELLFGDSCEDVATNLGAPNRIFFKSEDKMKIHSSSVNRQAQSKRSDIFFNYFTLGIDVLFDARTQTCKKFILHTNYPGHFNFNMYHRCEFQFQLLTDHQHSDNLDLMTPSKQDYVNISAYTKWDEISGSLSTSERPVVLHRASSTNTANPFGSTFCYGYQDLIFEVMPNNHIASVTLYNTAPPRQPAHTWQQHKMQDIRLTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU20</accession>
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        <submittedName>
            <fullName evidence="13">Protein nervous wreck</fullName>
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    </protein>
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        <name evidence="13" type="ORF">DMAD_07091</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
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    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:recycling endosome"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:neuromuscular synaptic transmission"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN NERVOUS WRECK"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SH3"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="11"/>
            <end position="289"/>
        </location>
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        <location>
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            <end position="725"/>
        </location>
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        </location>
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        </location>
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        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
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        <location>
            <begin position="497"/>
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        </location>
    </feature>
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        <location>
            <begin position="737"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="813"/>
            <end position="824"/>
        </location>
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        <location>
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            <end position="871"/>
        </location>
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        <location>
            <begin position="901"/>
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        </location>
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        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF99117.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1040" mass="114023" checksum="464B2ADA5A27CA79" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU22</accession>
    <name>A0AAU9FU22_DROMD</name>
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        <recommendedName>
            <fullName evidence="2">BACK domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="3 4">
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        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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        </lineage>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
    </feature>
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            <begin position="67"/>
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        </location>
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        <location>
            <begin position="318"/>
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        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF98986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="542" mass="61752" checksum="DE2DA3A4EBD04D06" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU25</accession>
    <name>A0AAU9FU25_DROMD</name>
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        <submittedName>
            <fullName evidence="6">TBC1 domain family member 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_07149</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="PH-GRAM2_TCB1D9_TCB1D9B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative TBC1 domain family member 8B"/>
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        <source>
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        <source>
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    <sequence length="1263" mass="143833" checksum="BA0F386037CE255C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU30</accession>
    <name>A0AAU9FU30_DROMD</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:29999"/>
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            <dbReference type="ChEBI" id="CHEBI:456216"/>
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        </reaction>
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    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
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            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
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        <property type="protein sequence ID" value="BFF98810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0004674">
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0008270">
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    <keyword evidence="8 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="107"/>
            <end position="373"/>
        </location>
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    <feature type="domain" description="AGC-kinase C-terminal" evidence="20">
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            <begin position="374"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="18">
        <location>
            <begin position="1377"/>
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        <location>
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    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="16">
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        <location>
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    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="711"/>
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    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="1048"/>
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    <feature type="coiled-coil region" evidence="15">
        <location>
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    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="1285"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="821"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="910"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
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            <end position="1012"/>
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    </feature>
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        <location>
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            <end position="1022"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1342"/>
            <end position="1359"/>
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    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <source>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="BFF98810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1850" mass="210560" checksum="B835BCBFEB6C7AED" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU31</accession>
    <name>A0AAU9FU31_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Talin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_07065</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10569">
        <property type="entry name" value="FERM_C_Talin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17089">
        <property type="entry name" value="FERM_F0_TLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17090">
        <property type="entry name" value="FERM_F1_TLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12150">
        <property type="entry name" value="talin-RS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000023">
        <property type="entry name" value="Rhea, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.10:FF:000010">
        <property type="entry name" value="Rhea, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.10:FF:000011">
        <property type="entry name" value="Rhea, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.10:FF:000012">
        <property type="entry name" value="Rhea, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000207">
        <property type="entry name" value="Rhea, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000005">
        <property type="entry name" value="Talin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000002">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000003">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1410.10:FF:000001">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.10:FF:000001">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.10:FF:000002">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.10:FF:000007">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000007">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000028">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.10">
        <property type="entry name" value="Talin, central domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036723">
        <property type="entry name" value="Alpha-catenin/vinculin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032425">
        <property type="entry name" value="FERM_f0"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035964">
        <property type="entry name" value="I/LWEQ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002558">
        <property type="entry name" value="ILWEQ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037438">
        <property type="entry name" value="Talin1/2-RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057346">
        <property type="entry name" value="Talin1/2_VBS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015224">
        <property type="entry name" value="Talin_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036476">
        <property type="entry name" value="Talin_cent_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054082">
        <property type="entry name" value="Talin_IBS2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049108">
        <property type="entry name" value="Talin_R4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054060">
        <property type="entry name" value="TLN1-like_RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015009">
        <property type="entry name" value="Vinculin-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19981:SF1">
        <property type="entry name" value="RHEA, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19981">
        <property type="entry name" value="TALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16511">
        <property type="entry name" value="FERM_f0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01608">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21896">
        <property type="entry name" value="Talin_IBS2B"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09141">
        <property type="entry name" value="Talin_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21692">
        <property type="entry name" value="Talin_R4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25177">
        <property type="entry name" value="Talin_VBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21865">
        <property type="entry name" value="TLN1-like_RS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08913">
        <property type="entry name" value="VBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00307">
        <property type="entry name" value="ILWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109880">
        <property type="entry name" value="A middle domain of Talin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47220">
        <property type="entry name" value="alpha-catenin/vinculin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109885">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50945">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="6">
        <location>
            <begin position="88"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="I/LWEQ" evidence="7">
        <location>
            <begin position="2297"/>
            <end position="2538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2672"/>
            <end position="2696"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1806"/>
            <end position="1854"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="2504"/>
            <end position="2533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="2682"/>
            <end position="2696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF99070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2696" mass="292683" checksum="D0D07BC328846C39" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU41</accession>
    <name>A0AAU9FU41_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein bric-a-brac 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_07440</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probably acts as a transcriptional regulator. Required for the specification of the tarsal segment. Also involved in antenna development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FU41"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003680">
        <property type="term" value="F:minor groove of adenine-thymine-rich DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007455">
        <property type="term" value="P:eye-antennal disc morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046660">
        <property type="term" value="P:female sex differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007478">
        <property type="term" value="P:leg disc morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
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        <property type="entry name" value="Bric a brac 2, isoform B"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="3 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="138"/>
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        </location>
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        <location>
            <begin position="601"/>
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            <begin position="629"/>
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            <begin position="1"/>
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            <begin position="409"/>
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            <begin position="453"/>
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        <location>
            <begin position="519"/>
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        <source>
            <dbReference type="PROSITE" id="PS50960"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF99574.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1019" mass="107436" checksum="52DD60E2BAF6F0F0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU46</accession>
    <name>A0AAU9FU46_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3 9">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07074</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">UTP--glucose-1-phosphate uridylyltransferase catalyzing the conversion of glucose-1-phosphate into UDP-glucose, a crucial precursor for the production of glycogen.</text>
    </comment>
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        <reaction evidence="8">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
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            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
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            <dbReference type="Rhea" id="RHEA:19890"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3 9"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="23"/>
        </location>
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            <name>UTP</name>
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        </ligand>
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        </ligand>
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        <location>
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        </location>
        <ligand>
            <name>substrate</name>
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        </location>
        <ligand>
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        </ligand>
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        </ligand>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF99085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="511" mass="57872" checksum="95CF91764E5D45B1" modified="2024-11-27" version="1">MLDVPHEGKVRGHQRVPSDSKEFHEVTKRDALRLLENDVDRLLQTVEEVRRPALKAEMGRFADLFGRFLQEEGPALDWNKIQKLPENAVMNYSNLKAPKNEQIRNMLDKLVVIKLNGGLGTSMGCHGPKSVIPVRSDLTFLDLTVQQIEHLNKTYDANVPLVLMNSFNTDEDTEKIVRKYKGFRVQIHTFNQSCFPRISREHFLPVAKDFDVEKDMEAWYPPGHGDFYDTFRNSGLLKKFIEEGREYCFLSNIDNLGATVDLNILNKLVGEERATTPVEFVMEVTDKTRADVKGGTLIQFENKLRLLEIAQVPPEHVDDFKSVKTFKFFNTNNIWANLASIERVLRERTLSMEIIVNNKTLETGLRVIQLETAVGSAMKCFDGAIGINVPRSRFLPVKKSSDLLLVMSNLYTLKNGSLVMSPQRMFPTTPLVKLGENHFSKVKEFLGRFANIPDIIELDHLTVSGDVTFGRGVSLRGTVIIIANHGDRIDIPAGAILENKIVSGNMRILDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU47</accession>
    <name>A0AAU9FU47_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07422</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="cyclin-dependent kinase 14 isoform X1"/>
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    <dbReference type="FunFam" id="3.30.200.20:FF:000007">
        <property type="entry name" value="Cyclin-dependent kinase 14, putative"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
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    <dbReference type="PANTHER" id="PTHR24056:SF246">
        <property type="entry name" value="ECDYSONE-INDUCED PROTEIN 63E, ISOFORM N"/>
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        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
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            <begin position="240"/>
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    <feature type="region of interest" description="Disordered" evidence="11">
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            <begin position="60"/>
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            <position position="269"/>
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        <source>
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        <source>
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    <sequence length="557" mass="62925" checksum="12887BDED5D71668" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU57</accession>
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            <fullName evidence="12">Neurabin-1</fullName>
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            <fullName evidence="14">Neurabin-I</fullName>
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        <alternativeName>
            <fullName evidence="13">Neural tissue-specific F-actin-binding protein I</fullName>
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        <alternativeName>
            <fullName evidence="15">Protein phosphatase 1 regulatory subunit 9A</fullName>
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        <name evidence="20" type="ORF">DMAD_07260</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="20 21">RF1</strain>
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        <subcellularLocation>
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    </dbReference>
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        <property type="entry name" value="Transcription Factor, Ets-1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF50156">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="1311"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="18">
        <location>
            <begin position="2125"/>
            <end position="2169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="81"/>
            <end position="123"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="193"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="259"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="982"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1062"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1445"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1713"/>
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    <feature type="region of interest" description="Disordered" evidence="17">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="2189"/>
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        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="84"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
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            <end position="212"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="17">
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    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
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    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="424"/>
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        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="493"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
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            <end position="1457"/>
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    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
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            <end position="2104"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the COE family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G5M8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11606">
        <property type="entry name" value="COE_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01175">
        <property type="entry name" value="IPT_COE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3180:FF:000003">
        <property type="entry name" value="Knot, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4280:FF:000001">
        <property type="entry name" value="transcription factor COE1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000762">
        <property type="entry name" value="transcription factor collier isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3180">
        <property type="entry name" value="Transcription factor COE1, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032200">
        <property type="entry name" value="COE_DBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038173">
        <property type="entry name" value="COE_DBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032201">
        <property type="entry name" value="COE_HLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038006">
        <property type="entry name" value="COE_IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003523">
        <property type="entry name" value="Transcription_factor_COE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018350">
        <property type="entry name" value="Transcription_factor_COE_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10747">
        <property type="entry name" value="TRANSCRIPTION FACTOR COE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16422">
        <property type="entry name" value="COE1_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16423">
        <property type="entry name" value="COE1_HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01345">
        <property type="entry name" value="COE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="IPT/TIG" evidence="12">
        <location>
            <begin position="295"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="453"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="476"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG03803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="571" mass="62144" checksum="538D9661B9AFD6B0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5Q3</accession>
    <name>A0AAU9G5Q3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nitric oxide-associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DMAD_02694</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G5Q3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052807">
        <property type="entry name" value="Mito_transl_resp_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46406">
        <property type="entry name" value="NITRIC OXIDE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46406:SF1">
        <property type="entry name" value="NITRIC OXIDE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFG03439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="182" mass="20373" checksum="133A37E05E7473A4" modified="2024-11-27" version="1">MIRPRAFRLRPEMSLLLGGLARIDILNLSGYEKQYDWLKVFVFASQNLPVMIADTLKAEAVYKRYLGTPFLGIPSASGDLEVRLKRWPGLQCKDQDIVVSNNNSNSGGNEARLNCDITLSSAGWLGLLLPDNSECSLRVWTPHSAGIYVRRPALIPLADRLVGKHIRNSLAYNTTKPFVFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5Q6</accession>
    <name>A0AAU9G5Q6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific protein-tyrosine kinase</fullName>
            <ecNumber evidence="1">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_02558</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="1"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007435">
        <property type="term" value="P:salivary gland morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05068">
        <property type="entry name" value="PTKc_Frk_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10370">
        <property type="entry name" value="SH2_Src_Src42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000318">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000053">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000044">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH2" evidence="13">
        <location>
            <begin position="1335"/>
            <end position="1427"/>
        </location>
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        <name type="scientific">Drosophila madeirensis</name>
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        <dbReference type="NCBI Taxonomy" id="30013"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="6">The CKK domain binds microtubules.</text>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Patronin, isoform M"/>
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        <property type="entry name" value="PATRONIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:16438"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotrimer; The trimer binds only one molecule of glutathione.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAPEG family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="5"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G5U2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.550:FF:000002">
        <property type="entry name" value="Microsomal glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.550">
        <property type="entry name" value="Membrane associated eicosanoid/glutathione metabolism-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023352">
        <property type="entry name" value="MAPEG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040162">
        <property type="entry name" value="MGST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10689">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10689:SF6">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161084">
        <property type="entry name" value="MAPEG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFG02930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="161" mass="18599" checksum="F9B53C74F114B05B" modified="2024-11-27" version="1">MSTNSSQTPSAQELYNLDNPIFCCYVFWATVLVAKMLLMSTLTAVQRFRYKIFPNQEDLFFKNTEVQFNDPHVERVRRAHRNDMENILPYFIMALIYISTNPDATVACNLFRVASVARLVHTLVYAVYPVPQPARLIAFASMQLITYYMAAVVALRTLRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G624</accession>
    <name>A0AAU9G624_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Glutathione synthetase</fullName>
            <shortName evidence="13">GSH-S</shortName>
            <ecNumber evidence="4 13">6.3.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_02371</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13557"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13558"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13 15">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the eukaryotic GSH synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.3" evidence="4 13"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG03020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G624"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004363">
        <property type="term" value="F:glutathione synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1080.10:FF:000011">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.50:FF:000001">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.80:FF:000009">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000141">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1760:FF:000007">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1760">
        <property type="entry name" value="Glutathione synthase, substrate-binding domain superfamily, eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1080.10">
        <property type="entry name" value="Glutathione Synthetase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005615">
        <property type="entry name" value="Glutathione_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014042">
        <property type="entry name" value="Glutathione_synthase_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014709">
        <property type="entry name" value="Glutathione_synthase_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014049">
        <property type="entry name" value="Glutathione_synthase_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037013">
        <property type="entry name" value="GSH-S_sub-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004887">
        <property type="entry name" value="GSH_synth_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01986">
        <property type="entry name" value="glut_syn_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130:SF0">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03917">
        <property type="entry name" value="GSH_synth_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03199">
        <property type="entry name" value="GSH_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001558">
        <property type="entry name" value="GSHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="6 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="11 13 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 13 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutathione synthase substrate-binding" evidence="16">
        <location>
            <begin position="210"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="372"/>
            <end position="381"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="376"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="405"/>
            <end position="408"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR001558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG03020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="491" mass="54786" checksum="CD5144B449A6AAC3" modified="2024-11-27" version="1">MSVDPNTPVLRNCIRLPLPEDELIEVTAKAKDYAIMHGAAMRSKSNFSEDSLNFAPFVLVPSSFPRKEFEKAVALQPIINRLMHNVAHDEEFITTTLAETIKVDEFTANLFNIYRKVLAHGFTQANSIGLLRSDYLGHVYEGCAIKQVEINTVASSFGGIATHLVQLQNFVLRELGHADKLPNMPHNNALAGLCDGMVKAWDIYAKPQSVILFIIEDVSYNICDQRFHEFYIRETYPHIKVLRRTLTDVHREGKLGLNKELLLSGGLEVAVIYFRAGYEPGHYHSQDEWDARYLMETSLAIKCPSIQYHLAGTKKVQQALAQPAVLERFINDPEEMKAVGKIFTGLYSLDDNEAGNASYEMALRTPEKFVLKPQREGGGNNVYGVDIPDALKRMSRVERSAWILMDLIHPPLTKGYMVRPGAEMPPTIVDMVSELGIFGVVIGDADHIIHNYQAGHMLRTKLSTANEGGVAAGLGALDSPYLIDSDDEDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G627</accession>
    <name>A0AAU9G627_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Synaptic vesicle 2-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_02327</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G627"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF35">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="378"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="412"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="441"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFG02966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="496" mass="53715" checksum="79E8FF6EEE37AD62" modified="2024-11-27" version="1">MGVMVEIMGVSLIMNQMKCDLQPTLEQQGILASAGFLGVVLSSHAMGFLADTWGRVTTLRYALLLAAVCSIISGFSVTIWMLIVFRFLTGFFISGGQACVFSLCGEFHGNGSRVRHVTLLSGFLCLSMIFAPAMAIGILPLRIETVVLGMRFSSWRVLLIANVSISLLALVGLSTLPETPKYMLVQGQGEAALETLRTIFSQNMRKPPSEYPVRAISLESGGASLSDVHGFCDAVRLVWRQTVPLFYRSRLVHTLNICTIQFLIYSMAQGIFMWFPTILDELGARGGDPTLLCRVLSEFDAQRPDNGTAVDTAQACAVDVDTNTYQVMIIIGASFTGIYLVFAYIIDYTGKRNLLMGWMALTMICLVGLHFVEQFALVVIALTVVMAIGNCGGLVSTIAMEFYPTHINAMGMCFIMMMGRLGAVVGSNLLGRLLFTSCDTVFWSLLGLVVFLCSLGYFLPEKPRAKRVATGTTTGTTTGTPSATATATPILAISSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G628</accession>
    <name>A0AAU9G628_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Unconventional myosin-Va</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_02653</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15470">
        <property type="entry name" value="Myo5_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01380">
        <property type="entry name" value="MYSc_Myo5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036103">
        <property type="entry name" value="MYSc_Myo5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF706">
        <property type="entry name" value="DILUTE CLASS UNCONVENTIONAL MYOSIN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50084">
        <property type="entry name" value="Myosin S1 fragment, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 8" id="KW-0518">Myosin</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="12">
        <location>
            <begin position="70"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="11">
        <location>
            <begin position="1467"/>
            <end position="1743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="8">
        <location>
            <begin position="650"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1102"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1382"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1571"/>
            <end position="1593"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="916"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="992"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1151"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1265"/>
            <end position="1317"/>
        </location>
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        <location>
            <begin position="1103"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="164"/>
            <end position="171"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1793" mass="206887" checksum="3B1D9F59FF6318D7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G635</accession>
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        <submittedName>
            <fullName evidence="5">Inactive serine protease scarface</fullName>
        </submittedName>
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        <name evidence="5" type="ORF">DMAD_02457</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BFG03127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
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        <property type="entry name" value="Trypsin_dom"/>
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    </dbReference>
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        <property type="entry name" value="ELASTASE 2 LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18399">
        <property type="entry name" value="CLIP_SPH_Scar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
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    </dbReference>
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    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
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        <location>
            <begin position="23"/>
            <end position="600"/>
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    </feature>
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        <location>
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            <end position="589"/>
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            <end position="263"/>
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            <begin position="153"/>
            <end position="168"/>
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        <location>
            <begin position="174"/>
            <end position="190"/>
        </location>
    </feature>
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        <location>
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            <end position="226"/>
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        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="600" mass="66220" checksum="9E6E1E7DA57D9B7F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G638</accession>
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        <submittedName>
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        <name evidence="18" type="ORF">DMAD_03025</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G638"/>
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        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000016">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000065">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.950:FF:000003">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000322">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.950">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
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    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
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    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043593">
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    <dbReference type="InterPro" id="IPR035836">
        <property type="entry name" value="ASAP1-like_SH3"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PH-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001849">
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    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
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    <dbReference type="PANTHER" id="PTHR45854:SF3">
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    <dbReference type="PANTHER" id="PTHR45854">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
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        <property type="entry name" value="ANK"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="16">
        <location>
            <begin position="313"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="17">
        <location>
            <begin position="430"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="634"/>
            <end position="666"/>
        </location>
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    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="1096"/>
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        </location>
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        <location>
            <begin position="281"/>
            <end position="302"/>
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    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="708"/>
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            <begin position="1045"/>
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            <begin position="286"/>
            <end position="295"/>
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        <location>
            <begin position="712"/>
            <end position="722"/>
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        <location>
            <begin position="745"/>
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            <begin position="802"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50115"/>
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        <source>
            <dbReference type="EMBL" id="BFG03902.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1160" mass="127536" checksum="3DF160E5FDD08064" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G644</accession>
    <name>A0AAU9G644_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">MAP/microtubule affinity-regulating kinase 3</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Serine/threonine-protein kinase par-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">DMAD_02833</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Regulates localization and activity of MITF by mediating its phosphorylation, promoting subsequent interaction between MITF and 14-3-3 and retention in the cytosol. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1. Phosphorylates PKP2 and KSR1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with MAPT/TAU. Interacts with DLG5 (via coiled-coil domain). Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5. Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ. Interacts with PKP2 (via N-terminus). Interacts with CDC25C. Interacts with KSR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
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    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9G644"/>
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        <property type="component" value="Chromosome E"/>
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        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="MARK1-3_C"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="STKc_MARK"/>
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        <property type="entry name" value="UBA_MARK_Par1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="KP78b, isoform A"/>
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        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
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        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
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    <dbReference type="FunFam" id="3.30.310.80:FF:000001">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
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    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
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    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR049508">
        <property type="entry name" value="MARK1-4_cat"/>
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    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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        <property type="entry name" value="UBA"/>
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        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
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        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 27" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="485"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="25">
        <location>
            <begin position="755"/>
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    </feature>
    <feature type="domain" description="KA1" evidence="26">
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    <feature type="region of interest" description="Disordered" evidence="23">
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    <feature type="region of interest" description="Disordered" evidence="23">
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    <feature type="region of interest" description="Disordered" evidence="23">
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    <feature type="region of interest" description="Disordered" evidence="23">
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            <begin position="1012"/>
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        <location>
            <begin position="1"/>
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        <location>
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        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="23">
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    <feature type="compositionally biased region" description="Polar residues" evidence="23">
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    <feature type="binding site" evidence="22">
        <location>
            <position position="514"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00054424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00063680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00074935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="BFG03629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1178" mass="128810" checksum="1B7699D461120DEE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G646</accession>
    <name>A0AAU9G646_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Integrin alpha-PS3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_02500</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G646"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001555">
        <property type="term" value="P:oocyte growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.130:FF:000015">
        <property type="entry name" value="integrin alpha-PS3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.930:FF:000005">
        <property type="entry name" value="Integrin, alpha 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF83">
        <property type="entry name" value="INTEGRIN ALPHA-PS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13 17" id="KW-0401">Integrin</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043092489" evidence="13">
        <location>
            <begin position="27"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1061"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="41"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="115"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="337"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="400"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="462"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha first immunoglubulin-like" evidence="15">
        <location>
            <begin position="510"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="16">
        <location>
            <begin position="651"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="245"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG03181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1118" mass="124443" checksum="134AA41D148034A9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G656</accession>
    <name>A0AAU9G656_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Solute carrier family 35 member F5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_02671</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC35F solute transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G656"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051:SF0">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35, MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="259"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG03408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="447" mass="50374" checksum="6846C44223A6D3B6" modified="2024-11-27" version="1">MLGRTQKLVLGITILILVDIVWVSSSELTKFLYKEAKFDKPFFCTYFKTSMFSIYLLVIGILAPWKESCERQNGTYAMMEQNAEDENYYTNQAGLGDPTYVPIRSAHLASTANASGNTLSGTESDDSSVRSVRFSKMAEVREMSAHEATDALLARLSYAASLRIRRQKTHHKTAKTALLFCLLWFVANYFFQLALEMDETAMITLVSSTSSFFIICMAAVFPSATGDKLTFTKVIAVGMNIGGVVAITMNDLHDTKMTRGVLLALFSAFFYASYLVFVKRKSDTEEKVDIPLFFGFVGLWNLMLLWPIFFILHFTKIETFEVPNQGQFALLFLNGLIGTVLAEALWLWGCFLTSSLIGTLAMSLQIPLAIVFDVMLKHEPYSRTFYMGSIPIFLALTLVALLMRNDDTDPLTKLFKIVYRKVCRCHKPNIVRVNDDEQQESLISNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G660</accession>
    <name>A0AAU9G660_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_03068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G660"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG03968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="256" mass="27529" checksum="E3019F64AE1BA5AA" modified="2024-11-27" version="1">MWNATEMENMWNAGAAAVAARRGHCQEMETLQPPSMTLPPAVPLALQGRKHGGQQSFGLNTLGRRYLQQSHQHSHYRRGCPLCGKFRYEAEAEVEAEDEVNPCDMSVESGSMRSREVPSEDANENGVLEGAEPATSPCPCTNNCDNEDGNGNHMIGEALLSDEANGNIQPATQEAINETTTTAISAVTDENDNAAAAATPSVTNEEEQPGGSQEQHQDLSSINENGFISMDMSKIIDRSGLPTYEGALKLESSGYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFS7</accession>
    <name>A0AAU9GFS7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Slo-interacting protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_13647</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GFS7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051971">
        <property type="entry name" value="E3_ubiquitin-PDZ_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15545">
        <property type="entry name" value="PDZ DOMAIN CONTAINING RING FINGER PROTEIN 3, 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15545:SF8">
        <property type="entry name" value="SLO-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="150"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="310"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG06726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="687" mass="76694" checksum="4354E2FC18442890" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFS8</accession>
    <name>A0AAU9GFS8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flocculation protein FLO11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_05001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GFS8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043919580" evidence="2">
        <location>
            <begin position="32"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="515"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="261"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="407"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="551"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="619"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="686"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="738"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="808"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="820"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG06506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="853" mass="89413" checksum="3AB4E2D7146522E5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFT0</accession>
    <name>A0AAU9GFT0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">calcium/calmodulin-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13627</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="2"/>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GFT0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007613">
        <property type="term" value="P:memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14086">
        <property type="entry name" value="STKc_CaMKII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000009">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000001">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000002">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta isoform 2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Ca/CaM-dep_prot_kinase-assoc"/>
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    <dbReference type="InterPro" id="IPR011009">
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    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08332">
        <property type="entry name" value="CaMKII_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="14"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG06661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="490" mass="55470" checksum="BD9DA0B7037EC788" modified="2024-11-27" version="1">MAAPAACTRFSDNYDIKEELGKGAFSIVKRCVQKSTGFEFAAKIINTKKLTARDFQKLEREARICRKLHHPNIVRLHDSIQEENYHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESVNHCHQNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGDHQAWFGFAGTPGYLSPEVLKKEPYGKSVDIWACGVILYILLVGYPPFWDEDQHRLYSQIKAGAYDYPSPEWDTVTPEAKNLINQMLTVNPNKRITASEALKHPWICQRERVASVVHRQETVDCLKKFNARRKLKGAILTTMLATRNFSSRSMITKKGDGSQVKESTDSSSTTLEDDDIKAARRQEIIKITEQLIEAINSGDFDGYTKICDPHLTAFEPEALGNLVEGIDFHKFYFENVLGKNCKAINTTILNPHVHLLGEEAACIAYVRLTQYIDKQGHAHTHQSEETRVWHKRDNKWQNVHFHRSASGKISGATTFDFIPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFT3</accession>
    <name>A0AAU9GFT3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Calcium-dependent secretion activator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13609</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990504">
        <property type="term" value="P:dense core granule exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016079">
        <property type="term" value="P:synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01234">
        <property type="entry name" value="PH_CADPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000007">
        <property type="entry name" value="Calcium-dependent secretion activator 2 isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.50:FF:000002">
        <property type="entry name" value="calcium-dependent secretion activator 2 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033227">
        <property type="entry name" value="CAPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057457">
        <property type="entry name" value="CAPS_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010439">
        <property type="entry name" value="MUN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014770">
        <property type="entry name" value="Munc13_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12166">
        <property type="entry name" value="CALCIUM-DEPENDENT SECRETION ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12166:SF8">
        <property type="entry name" value="CALCIUM-DEPENDENT SECRETION ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25341">
        <property type="entry name" value="C2_CAPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06292">
        <property type="entry name" value="MUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01145">
        <property type="entry name" value="DUF1041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51258">
        <property type="entry name" value="MHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="452"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="592"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="MHD1" evidence="15">
        <location>
            <begin position="1060"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1407"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1411"/>
            <end position="1430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG06627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1480" mass="166462" checksum="2711618EF5719C3A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFT4</accession>
    <name>A0AAU9GFT4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Paired box protein Pax-2-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_13623</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <evidence type="ECO:0000256" key="8">
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        <name type="scientific">Drosophila madeirensis</name>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51366"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFU7</accession>
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        <submittedName>
            <fullName evidence="4">Transcriptional activator protein Pur-beta</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ORF">DMAD_13661</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the PUR DNA-binding protein family.</text>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2450.30:FF:000001">
        <property type="entry name" value="Purine-rich element binding protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.700:FF:000004">
        <property type="entry name" value="Transcriptional activator protein Pur-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2450.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006628">
        <property type="entry name" value="PUR-bd_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12611">
        <property type="entry name" value="PUR-TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12611:SF0">
        <property type="entry name" value="PURINE-RICH BINDING PROTEIN-ALPHA, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04845">
        <property type="entry name" value="PurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00712">
        <property type="entry name" value="PUR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG06756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="275" mass="31009" checksum="EA53BFACD4FDB718" modified="2024-11-27" version="1">MSDLGSGDEGISGGSKYNVANMEGSSSRNDFDSSGKGGGTVEQELATKMLQIQSKRFYLDVKQNRRGRFIKVAEIGADGRRSQIYLALSTAAEFRDHLSSFSDYYASLGPPNTDNLPEDGKLKSEMMIKDYRRYYLDLKENARGRFLRVSQTITRGGPRSQIALPAQGMIEFRDALTDLLEEFGANDGGFKGDLPEERHMKVDNKNFYFDIGQNNRGVYMRISEVKNNFRTSITIPEKCWIRFRDIFNDYCDKMKKTSDPTTADINLPTSASSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFV0</accession>
    <name>A0AAU9GFV0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Kinesin-like protein unc-104</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_04956</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for presynaptic maturation, has a role in axonal transport of dense-core vesicles carrying synaptic vesicle precursors, components required for the morphological transformation of axonal growth cones to mature boutons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048490">
        <property type="term" value="P:anterograde synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021700">
        <property type="term" value="P:developmental maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051222">
        <property type="term" value="P:positive regulation of protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040012">
        <property type="term" value="P:regulation of locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051960">
        <property type="term" value="P:regulation of nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010975">
        <property type="term" value="P:regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008582">
        <property type="term" value="P:regulation of synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22705">
        <property type="entry name" value="FHA_KIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01233">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000001">
        <property type="entry name" value="Kinesin family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000004">
        <property type="entry name" value="Kinesin-like protein isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000204">
        <property type="entry name" value="kinesin-like protein unc-104 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
        <property type="entry name" value="Kinesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049780">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF10">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12473">
        <property type="entry name" value="DUF3694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="15">
        <location>
            <begin position="3"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="1548"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="399"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="964"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="593"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="399"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="409"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG06444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1681" mass="190509" checksum="00858B85CF12F87D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFV3</accession>
    <name>A0AAU9GFV3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
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                <person name="Yamamoto D."/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </location>
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            <end position="713"/>
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        </location>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG06635.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="749" mass="83199" checksum="3FD60078E85B609D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GG60</accession>
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        <submittedName>
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        </submittedName>
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        <name evidence="2" type="ORF">DMAD_05059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043706538" evidence="1">
        <location>
            <begin position="21"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG06609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="93" mass="9068" checksum="D1A0FD0FFCD7B337" modified="2024-11-27" version="1">MRRLLVLFLTLALLTGSAFGSLAFWRSLLGGGSVGYGSAGSSASSEANSVTINRGVGLGGGLGGGLGGGLGGGASYCCGIPRKQVQFGIGYSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAU9GG62_DROMD</name>
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        <submittedName>
            <fullName evidence="10">Paired box protein Pax-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_13623</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10 11">RF1</strain>
        </source>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="3" id="KW-0563">Paired box</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Paired" evidence="9">
        <location>
            <begin position="183"/>
            <end position="309"/>
        </location>
    </feature>
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        <location>
            <begin position="34"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="310"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="387"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="678"/>
            <end position="697"/>
        </location>
    </feature>
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        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
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        <location>
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            <end position="411"/>
        </location>
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        <location>
            <begin position="435"/>
            <end position="452"/>
        </location>
    </feature>
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        <location>
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            <end position="475"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51057"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG06650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="697" mass="74408" checksum="E4C9E165AE891076" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GG68</accession>
    <name>A0AAU9GG68_DROMD</name>
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        <submittedName>
            <fullName evidence="16">Calcium-dependent secretion activator</fullName>
        </submittedName>
    </protein>
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        <name evidence="16" type="ORF">DMAD_13609</name>
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    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
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        <subcellularLocation>
            <location evidence="11">Synapse</location>
        </subcellularLocation>
    </comment>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Munc13_1"/>
    </dbReference>
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        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PH_domain"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06292">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51258">
        <property type="entry name" value="MHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="452"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="592"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="MHD1" evidence="15">
        <location>
            <begin position="1060"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1463"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1467"/>
            <end position="1486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG06624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1536" mass="172325" checksum="D48CECA7A510DF05" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GG70</accession>
    <name>A0AAU9GG70_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14114">
        <property type="entry name" value="STKc_Twitchin_like"/>
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        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000097">
        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="5"/>
    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000614">
        <property type="entry name" value="Bent, isoform H"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001845">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:001892">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:000460">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000147">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:002083">
        <property type="entry name" value="Protein CBR-UNC-22"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="Titin a"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Titin isoform E"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="titin isoform X1"/>
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        <property type="entry name" value="titin isoform X1"/>
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        <property type="entry name" value="titin isoform X1"/>
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        <property type="entry name" value="twitchin isoform X2"/>
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        <property type="entry name" value="twitchin isoform X4"/>
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        <property type="entry name" value="Uncharacterized protein, isoform C"/>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Uncharacterized protein, isoform G"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform G"/>
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        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000876">
        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001033">
        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform I"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000553">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000559">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000748">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001016">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="71"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF151">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043182">
        <property type="entry name" value="PAIRED_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001523">
        <property type="entry name" value="Paired_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043565">
        <property type="entry name" value="PAX_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636:SF41">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00292">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00027">
        <property type="entry name" value="PAIREDBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00351">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00034">
        <property type="entry name" value="PAIRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51057">
        <property type="entry name" value="PAIRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0563">Paired box</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Paired" evidence="14">
        <location>
            <begin position="30"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="262"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="264"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="200"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="317"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="478"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="317"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="374"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG06665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="543" mass="58865" checksum="41D35C646B2B5D1F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EQS6</accession>
    <name>A0AAU9EQS6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CLIP domain-containing serine protease</fullName>
            <ecNumber evidence="11">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_07764</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The clip domain consists of 35-55 residues which are 'knitted' together usually by 3 conserved disulfide bonds forming a clip-like compact structure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10 12">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="11"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EQS6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035008">
        <property type="term" value="P:positive regulation of melanization defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.30:FF:000001">
        <property type="entry name" value="Serine protease 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000028">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000084">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022700">
        <property type="entry name" value="CLIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038565">
        <property type="entry name" value="CLIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12032">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00680">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51888">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 11 15" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043107994" description="CLIP domain-containing serine protease" evidence="12">
        <location>
            <begin position="23"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Clip" evidence="14">
        <location>
            <begin position="27"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="13">
        <location>
            <begin position="129"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF88870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="391" mass="43278" checksum="D6E78005454E62BE" modified="2024-11-27" version="1">MELKIVFGLLVLFIGERRQAYAQVFGYCTTPDESSGTCINLKECEYMYELVRRGSISTENRVFLQNSQCGYLNGQVLICCANNRRVNQQPVWGNQGQPRPQQPTTTQRATTSILLPQPPNCGENFGDRVVGGKETGKREFPWMVLIEYTKPGNVKGHHCGGSLINNRYVITAAHCVSAIPSDWQLTGVRLGEWDTSTNPDCTTEKNGRSDCNALYVDNPVVERVPHPQYPGNARDQLHDIALLRLRDPVSFTENISPVCLPSLASQRDASFLGRKMVVAGWGRTETNFTSNKKLKAEVDTVPLGDCNNRYASQRRIVTSNQICAGGVEGVDSCRGDSGGPLLLEDYNEGYSNYYITGVVSYGPTPCGLKGWPGVYTRVAAYLDFIENNVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ER63</accession>
    <name>A0AAU9ER63_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="4">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_08149</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="4"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9ER63"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05236">
        <property type="entry name" value="FAR-N_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09071">
        <property type="entry name" value="FAR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000458">
        <property type="entry name" value="Fatty acyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033640">
        <property type="entry name" value="FAR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF107">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03015">
        <property type="entry name" value="Sterile"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="458"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Thioester reductase (TE)" evidence="6">
        <location>
            <begin position="11"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acyl-CoA reductase C-terminal" evidence="5">
        <location>
            <begin position="351"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF89373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="478" mass="56018" checksum="F0848298BB7EF002" modified="2024-11-27" version="1">MDIYHDSVIFITGATGFVGKTLLEKLLWSFPQIKRIYMLIRPKGGVSVKQRFQEFLQNRIFERLRAEHPERLKKIFHFAGNIEDDNFGLSEPDRVRLCDEVNIIFHSAATVRFNECLKVAARVNSQATYNLLELCREMTQLRSFLYVSTAYCNPGRKYVDEEVYPTLPPVEWRQFLTCTKKIPEDYLNQLSVYIKGPHVNTYTFTKSIAEQIVNAYKDVIPIVIVRPSIVTAAYREPYPGWIDNIQAISGIMMEIGKGGISSILGDKDMICDIIPVDFVVNAMIMMINKAKLGSLSICNATSGVTNPITWQHLGELTMKWSRIYPTKRMIMFPNFKYRRSASQHELAVWMLHFVPALLMDLRTLLLSQKKRMVTPIARKFRQACLAGSFFSLNEWIFKNKSRYYFKEQIENGTFPMLYWNLEDLDYDEYVRRHMIGINKYLHRENFTVDSNKLMVTKVYWVWIFLHLCFYMLLIYVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZD3</accession>
    <name>A0AAU9EZD3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Heat shock protein 68</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_09526</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EZD3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000545">
        <property type="entry name" value="Endoplasmic reticulum chaperone BiP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000024">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="488"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="506"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF91185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="524" mass="57143" checksum="423E1C0D1E3E23F0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EZF4</accession>
    <name>A0AAU9EZF4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamate dehydrogenase [NAD(P)(+)]</fullName>
            <ecNumber evidence="3">1.4.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_09546</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamate + NAD(+) + H2O = 2-oxoglutarate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamate + NADP(+) + H2O = 2-oxoglutarate + NH4(+) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.3" evidence="3"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EZF4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000007">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000100">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
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    </evidence>
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        </source>
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        </source>
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        </source>
    </evidence>
    <sequence length="548" mass="60921" checksum="C87C751E35BEABEE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        </source>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="457" mass="52783" checksum="58E3D9EC5A84E8F1" modified="2024-11-27" version="1">MANLVARLTVKTGALIIQRTSVGVAVCRYAKYSSGPANPGGFSKKTRRLFSLLGGSAFSVAAVIAIIKLRALDNPVNAVSLKKRMRDDSELENVKLTARERRFIKFASVEFDDQLYMTPQDFLESVVEQEPRPRLKRRQLSSDEVDKYKENTPALKKGSTRLFRNLRDKGIVSYTEYLFLLSILTKPKSGFRIAFNMFDTDGNQRVDKDEFLVMERIFSGAWKEKHGEQESQELEHEVPPIEKDYVNDGEGLQRRHVVPTTLQLHFFGKRGTGVINYDNFYRFMDNLQTEVLELEFHEFSKGNSIISELDFAKILLRYTYLATDEYDVFLERLLERVKDEKGITFHDFRDFCHFLNNLDDFTIAMRMYTLADRAISKEEFSRAVKICTGYSLSQHLIDTVFAIFDADGDGLLSYKEFIAIMKDRLHRGFKSVAKSEGWDAFRFCVRQEMKTLMKSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F2R3</accession>
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        <recommendedName>
            <fullName evidence="8">Fork-head domain-containing protein</fullName>
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        <name evidence="9" type="ORF">DMAD_09357</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9 10">RF1</strain>
        </source>
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        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
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        </location>
    </feature>
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        </location>
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        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF90951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="722" mass="78093" checksum="65ED471FC14316B9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F2R6</accession>
    <name>A0AAU9F2R6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Annexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_08869</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="domain">
        <text evidence="6">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F2R6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032509">
        <property type="term" value="P:endosome transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000001">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000004">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000010">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF233">
        <property type="entry name" value="ANNEXIN B9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0041">Annexin</keyword>
    <keyword evidence="3 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 6" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF90319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="324" mass="36033" checksum="947474C69A0D825A" modified="2024-11-27" version="1">MSSAEYYPFKCTPTVYPADPFDPVEDAAILRKAMKGFGTDEKAIIEILARRGIVQRLEIAEAFKTSYGKDLISDLKSELGGKFEDVIVALMTPLPQFYAKELNDAISGLGTDEEAIIEILCTLSNYGIKTIAQFYEQSFGKPLESDLKGDTSGHFKRLCVSLVQGNRDENQGVDEAAAIADAQALHDAGEGQWGTDESTFNSILITRSYQQLRQIFLEYENLSGNDIEKAIKREFSGSVEKGFLAIVKCCKSKIDYFSERLHDSMAGMGTKDKTLIRIVVSRSEIDLGDIKEAFQNKYGKSLESWIKDDISGDYSYVLQCLASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F2S2</accession>
    <name>A0AAU9F2S2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Dynein beta chain ciliary</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_08878</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097729">
        <property type="term" value="C:9+2 motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001810">
        <property type="entry name" value="Cytoplasmic dynein 2 heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000667">
        <property type="entry name" value="Dynein axonemal heavy chain 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000003">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000003">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000003">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000002">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000219">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000002">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000001">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000411">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000002">
        <property type="entry name" value="Dynein heavy chain 9, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000002">
        <property type="entry name" value="Dynein heavy chain 9, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000049">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.130:FF:000001">
        <property type="entry name" value="Dynein, axonemal, heavy chain 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF8">
        <property type="entry name" value="DYNEINS HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
    <keyword evidence="10 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0243">Dynein</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="1864"/>
            <end position="2000"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2469"/>
            <end position="2614"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="1569"/>
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</entry>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1139" mass="121939" checksum="3BD82A76353F5E0B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F341</accession>
    <name>A0AAU9F341_DROMD</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_07759</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F341"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051640">
        <property type="entry name" value="GRB10-interact_GYF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445:SF36">
        <property type="entry name" value="FI03272P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445">
        <property type="entry name" value="GRB10 INTERACTING GYF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="115"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="475"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="520"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="647"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="610"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="115"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="483"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="527"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="566"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="647"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="658"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF88865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="697" mass="75043" checksum="B0160329E7B9C42E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F353</accession>
    <name>A0AAU9F353_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_07782</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F353"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035612">
        <property type="term" value="F:AP-2 adaptor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045747">
        <property type="term" value="P:positive regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000369">
        <property type="term" value="P:regulation of clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000099">
        <property type="entry name" value="Blast:Cyclin-G-associated kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1110:FF:000001">
        <property type="entry name" value="cyclin-G-associated kinase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000002">
        <property type="entry name" value="putative tyrosine-protein phosphatase auxilin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967:SF57">
        <property type="entry name" value="AUXILIN, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="54"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="16">
        <location>
            <begin position="584"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="15">
        <location>
            <begin position="1101"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="760"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="886"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="942"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="915"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="956"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="978"/>
            <end position="1008"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF88895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1177" mass="128378" checksum="E67CF0F72E9B9121" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F355</accession>
    <name>A0AAU9F355_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">procollagen-proline 4-dioxygenase</fullName>
            <ecNumber evidence="5">1.14.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_09586</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the P4HA family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.2" evidence="5"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F355"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF244">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="13">
        <location>
            <begin position="341"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF91266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="466" mass="53545" checksum="B9AA9D44A157E75A" modified="2024-11-27" version="1">MKLLRVEDELVRNMRSYVAKLKNKHQLVHQALSSMRAEERHMKMDYEAYLGNPLNSFRLIHRLHREWEQLVRYTSEAQQNARGHIEHAHRLRMLLPSALDLEEASRGIDSLVDIYELQPEELAAGTLVPGNTQPSAALSPLDCLALGNYCKNERNDRRAEAWFNASLERYDEDRLEYVVFGLSRKSIHNSWGMLLMKSQQETAGMSHLNTEGEENSLSKDIRKHYDSQMMYKRACSAVFRAPAHLHCRYNSSTTAFARIAPLKMEELSHDPYMVLFHDVVYESEMDWLLNAKQLKASLVGHYQYSAFRTSKEQHFHEYYNEAVVKSIHRRLTDMTGLSLIESDALTLINYGMGGHYDLHHDSHNYSEPNRILIGDRIATVLLYVGEVDSGGATIFPYINVSVRPTKGSAVLWYNLDDAAEINAKAMHAGCPVIVGSKYVLTKWINEMRQLFLTPCIRNKISNPKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F358</accession>
    <name>A0AAU9F358_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glucosidase 2 subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_09123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F358"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017177">
        <property type="term" value="C:glucosidase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039794">
        <property type="entry name" value="Gtb1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028146">
        <property type="entry name" value="PRKCSH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630:SF1">
        <property type="entry name" value="GLUCOSIDASE 2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630">
        <property type="entry name" value="N-LINKED OLIGOSACCHARIDE PROCESSING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12999">
        <property type="entry name" value="PRKCSH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosidase II beta subunit N-terminal" evidence="1">
        <location>
            <begin position="102"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF90634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="211" mass="24147" checksum="E68063E588079A9C" modified="2024-11-27" version="1">MRKTWVKRENIYYKPFYRQKSKMFLLLIFLVGISFIAYQLPTVHSPWNLQQLRRKHKEMMQSLSLGSKQRDVDPFDGGGATEARVAAAAGPDTSSAPDVEPIKIIRGTRLFDYDAYKPNFEGKFKCLDGSKEIAFDHLNDNYCDCEADGSDEPSTNACTNGRFYCRYQKRHITGRGRDKYVASSRVNDHVCDCCDGSDEWTTHVKCRNDCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCT0</accession>
    <name>A0AAU9FCT0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_11388</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EEIG family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FCT0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039931">
        <property type="entry name" value="EEIG1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456:SF1">
        <property type="entry name" value="C2 NT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="3">
        <location>
            <begin position="12"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="333"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="495"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="783"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="496"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="790"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF93551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="883" mass="96560" checksum="3FEB076E91163BEF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCT2</accession>
    <name>A0AAU9FCT2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Glycerol-3-phosphate dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="14">1.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_11331</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 14">
            <text>sn-glycerol 3-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Phospholipid metabolism; alpha-glycerophosphate cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.8" evidence="14"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FCT2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141152">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000004">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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    </dbReference>
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        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
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        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
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        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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    <dbReference type="NCBIfam" id="TIGR03376">
        <property type="entry name" value="glycerol3P_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728:SF8">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00957">
        <property type="entry name" value="NAD_G3PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12 13" id="KW-0520">NAD</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent N-terminal" evidence="15">
        <location>
            <begin position="7"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent C-terminal" evidence="16">
        <location>
            <begin position="195"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
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        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="270"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011009"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048683"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000114-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-2"/>
        </source>
    </evidence>
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            <dbReference type="PIRSR" id="PIRSR000114-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361243"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF93481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="353" mass="38676" checksum="2B6B4834C1913D56" modified="2024-11-27" version="1">MADKVNVCIVGSGNWGSAIAKIVGANAAALPEFEERVTMFVYEEMIDGKKLTEIINETHENVKYLKGHKLPPNVVAVPDLAEAAKNADILIFVVPHQFIPNFCKQLLGKIKPNAIAISLIKGFDKAEGGGIDLISHIITRHLKIPCAVLMGANLANEVAEGNFCETTIGCTDKKYGKVLRDLFQANHFRVVVVDDADAVEVCGALKNIVACGAGFVDGLKLGDNTKAAVIRLGLMEMIRFVDVFYPGSKLSTFFESCGVADLITTCYGGRNRRVSEAFVTSGKTIEELEKEMLNGQKLQGPPTAEEVNYMLKNKKLEDKFPLFTAIHKICTNQLKPKDLIDCIRNHPEHMQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCT3</accession>
    <name>A0AAU9FCT3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Eggshell protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_11336</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome U"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF93488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="124" mass="12144" checksum="E07EA614BB877A3A" modified="2024-11-27" version="1">MDGYYNGSGYGNIYAQGSSQGNSYGYGSSGMCSNGGCSNGSCANGSSSNGCCSMGCCSNGSCSNGSCLNGSCSNGSCISGRCTRMSAGGQFGGSFCSGGNCGRGAAGRSGGKPAWNSNWNERQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCU0</accession>
    <name>A0AAU9FCU0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Sodium/potassium-transporting ATPase subunit beta-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_11397</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the X(+)/potassium ATPases subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005890">
        <property type="term" value="C:sodium:potassium-exchanging ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1660:FF:000004">
        <property type="entry name" value="sodium/potassium-transporting ATPase subunit beta-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1660">
        <property type="entry name" value="Na, k-atpase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000402">
        <property type="entry name" value="Na/K_ATPase_sub_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038702">
        <property type="entry name" value="Na/K_ATPase_sub_beta_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523:SF28">
        <property type="entry name" value="NA_K-ATPASE BETA SUBUNIT ISOFORM 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523">
        <property type="entry name" value="SODIUM/POTASSIUM-DEPENDENT ATPASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00287">
        <property type="entry name" value="Na_K-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00390">
        <property type="entry name" value="ATPASE_NA_K_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0915">Sodium</keyword>
    <keyword evidence="16" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0740">Sodium/potassium transport</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025540"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="311" mass="36010" checksum="AA81A2489EF5BA8D" modified="2024-11-27" version="1">MADKKIGEYYSPPVKMGKWEGFKKFLWNSETSQCLGRTGSSWAKILLFYIIFYAALTGFFAAIFTVFYQTLDNEKPKWMLDNGLIGSNPGLGFRPMPPEANVESTLVWYESSKKDNYQYWVDETARFLKSYEDEDKQNHVNCSFEHPPPEGKVCGVEASSFAPCTIENNFGYHVARPCIFLKLNKIYNWIPEIYNDSKTLPDHMPEELKQHIKEKQQLRPNETGVVWVSCEGENPADVENIKARDYYPRMGFPRFYFPFKNIAGYIPPIVAVQFTVETGVLINIECKAWARNINHDRSDRRGSVHFELMVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAU9FCU5_DROMD</name>
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        <submittedName>
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        <name evidence="3" type="ORF">DMAD_11340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <location>
            <begin position="1"/>
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        </location>
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        <location>
            <begin position="21"/>
            <end position="123"/>
        </location>
    </feature>
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        <location>
            <begin position="226"/>
            <end position="251"/>
        </location>
    </feature>
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        <location>
            <begin position="54"/>
            <end position="111"/>
        </location>
    </feature>
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        <location>
            <begin position="226"/>
            <end position="243"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF93493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="251" mass="28222" checksum="FBBDB30B0BF03D8A" modified="2024-11-27" version="1">MRSVLFVFTAALLLWGNVAGREPPKAKAPQILEPSERSGAILSPKAKAPQIVEPAERRTGLNSPKADEPEAKEPKADEKEAGKLPKSDEAKASKSPEADAPKEPKSPKAEQSETANPPVEDIPEMVSKRSLIPGNWQLQGNSVVRWPPLSQQGITRRPHDLIVLERYWTTREKYTEEAIQRLDKKIPEMRKKYALSIQENKTLTQQLEAANLKLYDATLELKKAEQQREKCKDRKSEELDRESLPSTMGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <name evidence="3" type="ORF">DMAD_11354</name>
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    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">RF1</strain>
        </source>
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    </dbReference>
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            <end position="34"/>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="552" mass="61777" checksum="65E7EC7E290F78C9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCV7</accession>
    <name>A0AAU9FCV7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein obstructor-E</fullName>
        </submittedName>
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    <gene>
        <name evidence="8" type="ORF">DMAD_11355</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Chitin_bind-dev_reg"/>
    </dbReference>
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        <property type="entry name" value="CHITIN BINDING PERITROPHIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301:SF98">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="3"/>
    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
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        <location>
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        </location>
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        <location>
            <begin position="22"/>
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        </location>
    </feature>
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        <location>
            <begin position="22"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
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        </location>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF93511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="242" mass="26883" checksum="C2810CE7B6870120" modified="2024-11-27" version="1">MAKIVISALLCLAMFGSMVLCSPECPFPNGRFASGDQCDAYTECLDDVPTAKLCPDGLLFHQRTKATGECTYAPYSTCKERARLQPANGTDECPRQFGFYPNGDETKCGVYRNCAHGVASITKCPEGLAFNEETYQCDWPDLVGSCNAEAFLGFNCPAAEPVDAIAPEVDVGPEGELRYYRHPQTCKKYFVCVNGHPRLYNCGKYLAFNSESKLCDFYNKVPECYALLKEKQRLKAEKQAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCV8</accession>
    <name>A0AAU9FCV8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome c oxidase assembly factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_11353</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FCV8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014807">
        <property type="entry name" value="Coa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47148">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47148:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08695">
        <property type="entry name" value="Coa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF93508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="25611" checksum="0278AA48F904BA2B" modified="2024-11-27" version="1">MPTFKMRLTLPSRRLLANLCVCGAMASISGLAYLHWKLEDRVRQADFYQLAIKTLRQHGGAVRLLGEPIKESGISLTNTQNSCDGDRAQMQVSVHGSKDKGTIFFWATNNREKGVWLIDRLELETKQHPNTRFLLKKPMDKALDDIVHNEENEQDHEHIVVPEPKPIRPQMNQHTEQPAQGQNPYDHPGYQEGGVGQKPNQPKTLHQHQGQEPAPYIQTADGGRMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCW1</accession>
    <name>A0AAU9FCW1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dynein axonemal assembly factor 1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Defective transmitter-recycling protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Leucine-rich repeat-containing protein 50 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_11394</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cilium-specific protein required for cilia structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNAAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000166">
        <property type="entry name" value="Dynein assembly factor 1, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000331">
        <property type="entry name" value="Dynein assembly factor 1, axonemal homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF9">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="310"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="527"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1061"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1297"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1497"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1604"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="727"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="845"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1209"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1437"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="312"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="336"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1078"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1506"/>
            <end position="1517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1611"/>
            <end position="1626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF93559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1688" mass="190809" checksum="320123F490D6D074" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCW2</accession>
    <name>A0AAU9FCW2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="24">Glutamate receptor ionotropic kainate 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_11375</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FCW2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13714">
        <property type="entry name" value="PBP2_iGluR_Kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000297">
        <property type="entry name" value="Glutamate receptor IIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000117">
        <property type="entry name" value="Glutamate receptor, ionotropic kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000143">
        <property type="entry name" value="Probable glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10 24" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="21" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043998134" evidence="21">
        <location>
            <begin position="19"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="552"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="624"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="816"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="22">
        <location>
            <begin position="419"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="23">
        <location>
            <begin position="429"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="876"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="880"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="897"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="507"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="512"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="679"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="729"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="17">
        <location>
            <position position="657"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="17">
        <location>
            <position position="775"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="741"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="BFF93534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="909" mass="104249" checksum="78912E4048D6484C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FCW5</accession>
    <name>A0AAU9FCW5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chloride intracellular channel protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_11422</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FCW5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF93596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="172" mass="18481" checksum="F249CFF7ED2C9A84" modified="2024-11-27" version="1">MPAEGAKPTGDSGEVENVAALGAEQGDAGNQTTTDGSKAAPDSSKGAAEDEKDSKEEKAEKGEEDTTSKDPFYYGNGSPTTLDDAQTPGTNPTLPPETTTTTGIMDLMTYMPEDNAGDPFVSPGAAHRPGSRHVKAHDGFHSLTTEQYWAHWNDRFTTKKSRNGHHWRDLMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FD14</accession>
    <name>A0AAU9FD14_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dynein axonemal assembly factor 1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Defective transmitter-recycling protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Leucine-rich repeat-containing protein 50 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_11394</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cilium-specific protein required for cilia structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNAAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000166">
        <property type="entry name" value="Dynein assembly factor 1, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000331">
        <property type="entry name" value="Dynein assembly factor 1, axonemal homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF9">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="310"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="527"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1061"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1297"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1497"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1600"/>
            <end position="1644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1662"/>
            <end position="1684"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="727"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="845"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1209"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1437"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="312"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="336"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1078"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1506"/>
            <end position="1517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1609"/>
            <end position="1623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1628"/>
            <end position="1644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1663"/>
            <end position="1673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF93562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1714" mass="193663" checksum="2CC03FDD130B0041" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FD50</accession>
    <name>A0AAU9FD50_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_11444</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 24">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="CDD" id="cd05739">
        <property type="entry name" value="IgI_3_RPTP_IIa_LAR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14554">
        <property type="entry name" value="R-PTP-LAR-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14553">
        <property type="entry name" value="R-PTPc-LAR-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000010">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000002">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000001">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase F isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001111">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001197">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001219">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001274">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001000">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001102">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001130">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001015">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001564">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001523">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001191">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF6">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043863222" description="protein-tyrosine-phosphatase" evidence="17">
        <location>
            <begin position="32"/>
            <end position="2043"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="49"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="153"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="247"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="337"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="432"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="530"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="626"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="724"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="828"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="925"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="1022"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="1117"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="18">
        <location>
            <begin position="1487"/>
            <end position="1742"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="19">
        <location>
            <begin position="1659"/>
            <end position="1733"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="18">
        <location>
            <begin position="1774"/>
            <end position="2033"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="19">
        <location>
            <begin position="1951"/>
            <end position="2024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1359"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1359"/>
            <end position="1371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="BFF93624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2043" mass="230504" checksum="B54D968DDA546FC9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FD51</accession>
    <name>A0AAU9FD51_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Fructose-1,6-bisphosphatase isozyme 2</fullName>
            <ecNumber evidence="8">3.1.3.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">D-fructose-1,6-bisphosphate 1-phosphohydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">D-fructose-1,6-bisphosphate 1-phosphohydrolase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Muscle FBPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">DMAD_11437</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homotetramer. Interacts with ALDOA; the interaction blocks inhibition by physiological concentrations of AMP and reduces inhibition by Ca(2+). Interacts with alpha-actinin and F-actin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Myofibril</location>
            <location evidence="4">Sarcomere</location>
            <location evidence="4">Z line</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 24">Belongs to the FBPase class 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="8"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FD51"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005986">
        <property type="term" value="P:sucrose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00354">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000001">
        <property type="entry name" value="Fructose-1,6-bisphosphatase class 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000005">
        <property type="entry name" value="Fructose-1,6-bisphosphatase isozyme 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01855">
        <property type="entry name" value="FBPase_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044015">
        <property type="entry name" value="FBPase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000146">
        <property type="entry name" value="FBPase_class-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033391">
        <property type="entry name" value="FBPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028343">
        <property type="entry name" value="FBPtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020548">
        <property type="entry name" value="Fructose_bisphosphatase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006778">
        <property type="entry name" value="PRK09293.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556:SF1">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00316">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18913">
        <property type="entry name" value="FBPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500210">
        <property type="entry name" value="FBPtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000904">
        <property type="entry name" value="FBPtase_SBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00115">
        <property type="entry name" value="F16BPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00124">
        <property type="entry name" value="FBPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="17 24" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="15" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 24" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class I N-terminal" evidence="25">
        <location>
            <begin position="13"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class 1 C-terminal" evidence="26">
        <location>
            <begin position="206"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00010941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00037516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00038670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00040321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00042757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00043165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU000508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF18913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="BFF93615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="335" mass="36227" checksum="141F29BC85A5E11C" modified="2024-11-27" version="1">MSQQGPVFDSNAMTLTRFVLQEQRKFKNATGDLSQLLNCIQTAIKATASAVRKAGIAKLHGIAGDVNVQGEEVKKLDVLSNELFINMLKSSYTTCLMVSEENEHVIEVETDKQGKYIVCFDPLDGSSNIDCLVSIGSIFAIYRKKSEGVPTVEDALQPGNQLVAAGYALYGSATAIVLGLGTSGVNGFTYDPAIGEFILTEPNMRVPEKGKIYSINEGYASDWEAGVLNYVAAKKDPSKGKPYGARYVGSMVADVHRTIKYGGIFIYPATKSAPNGKLRLLYECNPMAYLMIQAGGLASDGKISILDIVPKKIHERSPIFLGSKADVEEALSYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FD58</accession>
    <name>A0AAU9FD58_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_11444</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 24">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="CDD" id="cd05739">
        <property type="entry name" value="IgI_3_RPTP_IIa_LAR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14554">
        <property type="entry name" value="R-PTP-LAR-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14553">
        <property type="entry name" value="R-PTPc-LAR-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000010">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000002">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000001">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase F isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001111">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001197">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001219">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001274">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001000">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001102">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001130">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001015">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001564">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001523">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001191">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF6">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
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    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
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        <location>
            <begin position="32"/>
            <end position="2034"/>
        </location>
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        <location>
            <begin position="35"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
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            <begin position="139"/>
            <end position="223"/>
        </location>
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            <begin position="233"/>
            <end position="315"/>
        </location>
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            <begin position="323"/>
            <end position="413"/>
        </location>
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        <location>
            <begin position="423"/>
            <end position="517"/>
        </location>
    </feature>
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        <location>
            <begin position="521"/>
            <end position="612"/>
        </location>
    </feature>
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        <location>
            <begin position="617"/>
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        </location>
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        <location>
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        </location>
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            <end position="1009"/>
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        <location>
            <begin position="1108"/>
            <end position="1210"/>
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            <begin position="1478"/>
            <end position="1733"/>
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            <end position="1724"/>
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        <location>
            <begin position="1942"/>
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        <location>
            <begin position="1350"/>
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        </location>
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            <begin position="1350"/>
            <end position="1362"/>
        </location>
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        </source>
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            <dbReference type="ARBA" id="ARBA00010504"/>
        </source>
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        <source>
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        </source>
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        <source>
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        </source>
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        </source>
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        <source>
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        </source>
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        </source>
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        </source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2034" mass="229288" checksum="1B8082E844908771" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FD62</accession>
    <name>A0AAU9FD62_DROMD</name>
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        <submittedName>
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        <name evidence="2" type="ORF">DMAD_11422</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">RF1</strain>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="149" mass="18352" checksum="14271E3BD9F37260" modified="2024-11-27" version="1">MLQSQRQQREKELQGRLQQEEELQERLRREKEHQGRLQREKELQGRLQEEKELKVRQPQQMAHLERLQLELQVRLQSEMVLQVRLQQEKMELQVRLQQEKMELQVRLPQGQTQPLQMERRQRSLQLVALRTSRLQPMELPVRRQQVKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FD64</accession>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="16 17">RF1</strain>
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    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CHORION TRANSCRIPTION FACTOR CF2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-C2H2"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
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        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="15">
        <location>
            <begin position="17"/>
            <end position="99"/>
        </location>
    </feature>
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        <location>
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            <end position="245"/>
        </location>
    </feature>
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        </location>
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            <end position="302"/>
        </location>
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            <end position="330"/>
        </location>
    </feature>
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        <location>
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            <end position="358"/>
        </location>
    </feature>
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        <location>
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            <end position="386"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
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            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
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            <end position="481"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="485"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="107"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="140"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="84"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="126"/>
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        </location>
    </feature>
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            <begin position="153"/>
            <end position="166"/>
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        <location>
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            <begin position="2081"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006255"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF93605.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2094" mass="221943" checksum="5A69EC0A0922C87E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FD87</accession>
    <name>A0AAU9FD87_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_11444</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 24">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF93625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="CDD" id="cd05739">
        <property type="entry name" value="IgI_3_RPTP_IIa_LAR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14554">
        <property type="entry name" value="R-PTP-LAR-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14553">
        <property type="entry name" value="R-PTPc-LAR-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000010">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000002">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000001">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase F isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001111">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001197">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:001219">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:001274">
        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X1"/>
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        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X3"/>
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        <property type="entry name" value="tyrosine-protein phosphatase Lar isoform X3"/>
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        <property type="entry name" value="Uncharacterized protein, isoform A"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="12"/>
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    <dbReference type="Gene3D" id="3.90.190.10">
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        <property type="entry name" value="FN3_sf"/>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_I-set"/>
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    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
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    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
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    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
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    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
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    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
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    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="fn3"/>
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        <property type="entry name" value="I-set"/>
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    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
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    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
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    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
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    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
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    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
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    </dbReference>
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        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043829614" description="protein-tyrosine-phosphatase" evidence="17">
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            <begin position="32"/>
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        <location>
            <begin position="35"/>
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    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="139"/>
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    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="233"/>
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    </feature>
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        <location>
            <begin position="323"/>
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    </feature>
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        <location>
            <begin position="418"/>
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    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="516"/>
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    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="612"/>
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    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="710"/>
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    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="814"/>
            <end position="910"/>
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    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="911"/>
            <end position="1004"/>
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    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="1008"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="1103"/>
            <end position="1205"/>
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    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="18">
        <location>
            <begin position="1473"/>
            <end position="1728"/>
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    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="19">
        <location>
            <begin position="1645"/>
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    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="18">
        <location>
            <begin position="1760"/>
            <end position="2019"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="19">
        <location>
            <begin position="1937"/>
            <end position="2010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1345"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1345"/>
            <end position="1357"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010504"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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                <person name="Yamamoto D."/>
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</entry>
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    <accession>A0AAU9FLA9</accession>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Yamamoto D."/>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1357"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1666"/>
            <end position="1676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF96381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1701" mass="190658" checksum="FF09B8306921C8D7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLE6</accession>
    <name>A0AAU9FLE6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_05190</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLE6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043448598" description="Kazal-like domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="3">
        <location>
            <begin position="296"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF96583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="357" mass="39558" checksum="515D6C687BEA538B" modified="2024-11-27" version="1">MRTLLVIALLSLSLAQATEVRQPTEATTTTGKPQQVELPEHIQTLITNHIHHVLENVKNKPQKPHTLTGTVGAASKGSVSQVVEPFSGLLPPLAHSSDVHIPAPDQPTASPALQPTLQSLQANDPQVRVINHEATGSLLHQGRLVAQNKVVMPPVPYVVLSLPGQKPVGVADKVNKLQQHVSQVMKQAQLHIPLAIQEAIKIKKEQQNQQNQHKQQEQSTDEVKDQNKEEQKGQQPDKGKYQDNEIETSPQHDSSSTPLPTKSKTGRSLDKWHHHANQVHIPQLIAEHGEEIELGKCSFQCPRQALSICASNGKCVVNFPGQCELSQWNCFNTKNVFHQVHDSQCQNTIKCYKRDMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLF3</accession>
    <name>A0AAU9FLF3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Adenosine kinase</fullName>
            <shortName evidence="10">AK</shortName>
            <ecNumber evidence="3 10">2.7.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Adenosine 5'-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_05251</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>adenosine + ATP = AMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20824"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 3 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.20" evidence="3 10"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLF3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004001">
        <property type="term" value="F:adenosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01168">
        <property type="entry name" value="adenosine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000006">
        <property type="entry name" value="Adenosine kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001805">
        <property type="entry name" value="Adenokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769:SF3">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00989">
        <property type="entry name" value="ADENOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 10" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="11">
        <location>
            <begin position="40"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601805-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF96662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="348" mass="38248" checksum="C7B4B7DA8485A647" modified="2024-11-27" version="1">MWDRSSTVREGILVGCGNPLLDISASVPMDFLKKYEMNEDDAILAEERHMPIYRELVDGFQAEFLAGGSVQNSLRIAQWILGQPKVAVFFGCVGEDEYADILMEKARSAGLDVHYQIKKDVPTGTCAVLITGTHRSLCANLAAANNFTIDHLDQPHNKTLVENALYYYISGFFLTVNPPSIMQVAATALAKQRPFLMNLSAPFISQFYMAPLLAVMPYVDIIFGNEAEAHAFATAQGWPTEDLREIGKRLVALDKLNPARPRIAILTQGCDPVLLIQRDSVEEFPVTRLAVHEIVDTNGAGDAFVGGFLSQFVQGKSLDVCIRCGNYAAGHIIKNPGCTYTGEPQFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLF6</accession>
    <name>A0AAU9FLF6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_05101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLF6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF96462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="136" mass="15575" checksum="724C0C7E915DF7D4" modified="2024-11-27" version="1">MTDTIRGTLQEEFRPGFMELMAAITEAMTPLRQEGEIIQDLVWADDPNGREGGQTAPECQKQSTGTKHRCSGEHQEQILQEPPRKQVKRGQDWSRIRDDGELDKRPGKQERKPAGDGRARRCKTPYERREEGRISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLG0</accession>
    <name>A0AAU9FLG0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Collagen alpha-1(XVIII) chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_05327</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000116">
        <property type="entry name" value="Serine protease P16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="26"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF96692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="276" mass="30381" checksum="A5E41049F245BA80" modified="2024-11-27" version="1">MHPIFTNILKIFIVTSQPCTVRNPKIVGGSEAERNEMPFMVSLMRRGGHFCGGTIISERWILTAGHCICNGLQKFMKPAQIQGVVGLHSIREYLNGIGNGPEALRVAFKNIVPHPQYDCNSVQHDIALLEVERPISFTANVQPSCVGSDKGQRSLEQEYGTVSGWGWTHENQAEGDRADVLRKATVKIWNNEDCERSYRALGKSNSIGETQLCAGYENGQIDSCWADSGGPLMSKEHHLVGVVSTGIGCARPGLPGIYTRVSKYVPWIQNVIGSWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLH4</accession>
    <name>A0AAU9FLH4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptide-methionine (S)-S-oxide reductase</fullName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Peptide-methionine (S)-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05095</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLH4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1060.10:FF:000004">
        <property type="entry name" value="Peptide methionine sulfoxide reductase A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049006">
        <property type="entry name" value="MsrA_helical"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774:SF1">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE MSRA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20939">
        <property type="entry name" value="MsrA_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="5">
        <location>
            <begin position="34"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Selenoprotein methionine sulfoxide reductase A helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF96453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="239" mass="27144" checksum="FEA8C6E7B2192B4F" modified="2024-11-27" version="1">MFAHFFRNFKRTVRNEQKELSILPVHHVNIPKATATFGMGCFWGAESLYGATRGVLRTTVGYAGGSSELPTYRKLGDHTEVLEIDYDPTVISFKELLDLFWNNHEYGLTTPIKRQYASLILYHDDEQKKMAVTSKLEEQERRAPELITTEVAPKVNFYAAEAYHQKYRLQGHKDLATSLNLSPTLLQSSYVATKLNGYLAGVGGMEQFKSEVDTLGLTPTQRQYCSYHVEQNEGQGLYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FLH9</accession>
    <name>A0AAU9FLH9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4789 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_05125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF96490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FLH9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031993">
        <property type="entry name" value="DUF4789"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21177:SF7">
        <property type="entry name" value="GH11627P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21177">
        <property type="entry name" value="IP06524P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16033">
        <property type="entry name" value="DUF4789"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4789" evidence="1">
        <location>
            <begin position="81"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF96490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="493" mass="54150" checksum="146F0CE8EEF1D82E" modified="2024-11-27" version="1">MTRRKLLICWAALQAGSTMITCLLLMLTTSQPGNQVAAGIVPPPWSDPATNPCASMSGGWQLLYWTPLKKCFKIFTLGYPCPDTMELSPTPSNAKRKNLPAAECRCPPGTALSPLTSSVCHKLYERGPCQRTEYFHPLLDQAKRTGNRWGTCKRPLQCVDGMIFWPRDNKCYARHRRGPCSRGKLLVRNEEGLAECRCEDAGELATYYSPAEDSCHEHYTKGPCSTPGHIFMPGGRCDCQQHLPQYYVPQQMCFELDTTGPCPPGHIFRIPDNTEVATGGSLQLLPRAKCLCKEGYVPWADGNCYRLYTRGPCGANEFLVNATSCVHNFCGKSRLYFPAEDSCYRIGSQGPCSLYQVVIFDFTARPSLDGISYNGMCGCAGALTNLDQHCSGLGSGLGSDTDKEQNICESTPGMVEINGQCHKLYTRGPCGAGQWLQPLKTHSQTNTATLAYASRAKCVCRPGYTPTEADQRGQSNCSARSVSLARWLLDIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTU6</accession>
    <name>A0AAU9FTU6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Proton-coupled amino acid transporter-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_07054</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTU6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF460">
        <property type="entry name" value="PROTON-COUPLED AMINO ACID TRANSPORTER 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="355"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="400"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="423"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="467"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="81"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF99057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="494" mass="54347" checksum="025610F34225DF6F" modified="2024-11-27" version="1">MQPEEKIPTVVSSGYDSEKTQGGRNNASIGQPKFIRSDMADVPVQQAAGSTLPLVITRKKGGGDSDDGNYNPFEHRKVEHPTSDLETFVHLLKGSLGSGILAMPMAFSHAGLWFGLVATFAVGTLCTYCVHVLVKCAHILCRRRKIPMMGFADVAEQAFLDGPPALNRWSRFIRFMVNTFLVLDLLGCCCIYLVFVATNVEQVVSVYLETVLSVRVWIMIVTVPLIFMCLVRNLKFLTPFSMIANILMFVGIVITFIYMFTDLPAPSERPGLVAVSEWPLFFGTVIFALEGIGVVMSLENDMRNPTHFIGCPSVLNFGMGLVIALYTLVGFFGFLKYGPDTEASITLNLPVEDKLAQSVKLMIAIAIFFTFTLQFYVPVSILWKGLEHKIRPERQNISEYGLRVALVVLCGGIAVALPNLGPFISLIGAVCLSTLGMIVPATIELAVYHEDPGYGRFNWRLWKNSGLILFGVVGFVAGTYVSIIEFHAEFSGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTV3</accession>
    <name>A0AAU9FTV3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">PH-sensitive chloride channel 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_07374</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTV3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099095">
        <property type="term" value="F:ligand-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18987">
        <property type="entry name" value="LGIC_ECD_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19049">
        <property type="entry name" value="LGIC_TM_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000021">
        <property type="entry name" value="glutamate-gated chloride channel isoform X12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000020">
        <property type="entry name" value="glutamate-gated chloride channel isoform X12"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
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        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
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        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="640"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="668"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="704"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1003"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="13">
        <location>
            <begin position="449"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="14">
        <location>
            <begin position="647"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="128"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="826"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="907"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="832"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="863"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF99469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1028" mass="114111" checksum="ECF43EA5E5ECAF40" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTV5</accession>
    <name>A0AAU9FTV5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Protein nervous wreck</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07091</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTV5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0061024">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051495">
        <property type="term" value="P:positive regulation of cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902905">
        <property type="term" value="P:positive regulation of supramolecular fiber organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11761">
        <property type="entry name" value="SH3_FCHSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000107">
        <property type="entry name" value="FCH and double SH3 domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000039">
        <property type="entry name" value="FCH and double SH3 domains protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000155">
        <property type="entry name" value="FCH and double SH3 domains protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
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    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035460">
        <property type="entry name" value="FCHSD_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF21">
        <property type="entry name" value="PROTEIN NERVOUS WRECK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="12">
        <location>
            <begin position="11"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="545"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="638"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="431"/>
            <end position="542"/>
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</entry>
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        <source>
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    <sequence length="1032" mass="113329" checksum="BA931553D9732A39" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTW9</accession>
    <name>A0AAU9FTW9_DROMD</name>
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        <recommendedName>
            <fullName evidence="18">Tequila</fullName>
        </recommendedName>
    </protein>
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        <name evidence="16" type="ORF">DMAD_07097</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <dbReference type="GO" id="GO:0035008">
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFF99127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2295" mass="251865" checksum="1FE5069D344AF1BB" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTX1</accession>
    <name>A0AAU9FTX1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IST1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Charged multivesicular body protein 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_06989</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">ESCRT-III-like protein involved in cytokinesis, nuclear envelope reassembly and endosomal tubulation. Is required for efficient abscission during cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with CHMP1A, CHMP1B, VPS4A and VTA1. Interacts with SPAST, STAMBP, and USP8. May interact with VPS37B. May associate with the ESCRT-I complex. Interacts with MITD1, in competition with VSP4. Interacts with SPART (via MIT domain); leading to the recruitment of SPART to midbodies. Interacts with SPAST.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the IST1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF98973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTX1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.60:FF:000001">
        <property type="entry name" value="IST1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.60">
        <property type="entry name" value="Vacuolar protein sorting-associated protein Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005061">
        <property type="entry name" value="Ist1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042277">
        <property type="entry name" value="IST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161">
        <property type="entry name" value="IST1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161:SF5">
        <property type="entry name" value="IST1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03398">
        <property type="entry name" value="Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="237"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="8"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="293"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="334"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="379"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFF98973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="391" mass="43230" checksum="10A6067B83E5EDC7" modified="2024-11-27" version="1">MFSSGPNYNKLKTNLRLALNRLKLLEKKKAELTQKSRKEIADYLATGKTERARIRVEHIIREDYLVEAMEMVEMYCDLLLARFGLITQMKELDSGIAEPVASLVWVCPRLQSDIAELKIISDIFITKYGPQFAEHNRTATGDHSVSEKLMHKLTLQAPPKLLVENYLIAIAKNYNIEYEPDPQVMQEEQRPEHHLIDLSDRNNLSGSGGPPPQMGFIGYPAVPPLPNMPMPPTGKPFNYPPFGGGGGGGGGAAACAGPQPPPPFAYNIPPNQPPQPTMPSMVPGKCAEEKDLNTNFINRQKPNNDPPPRYTSINPVNLQNANKPKPQPRSKLPPGGPTLPSAPPALDLPSLPNVPLDLPDIPPGPAGKKEDEDEIDFDELSRRFENLKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FTX6</accession>
    <name>A0AAU9FTX6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3 9">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_07074</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">UTP--glucose-1-phosphate uridylyltransferase catalyzing the conversion of glucose-1-phosphate into UDP-glucose, a crucial precursor for the production of glycogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19890"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3 9"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FTX6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00897">
        <property type="entry name" value="UGPase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000001">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000002">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016267">
        <property type="entry name" value="UDPGP_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43511">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000806">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-2"/>
        </source>
    </evidence>
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    <dbReference type="FunFam" id="3.30.420.10:FF:000019">
        <property type="entry name" value="RNA exonuclease NEF-sp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034922">
        <property type="entry name" value="REX1-like_exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF82">
        <property type="entry name" value="RNA EXONUCLEASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="8">
        <location>
            <begin position="391"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="76"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF98988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="720" mass="81220" checksum="5CD15AB5A86EB861" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU65</accession>
    <name>A0AAU9FU65_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein SCAI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_07313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FU65"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022709">
        <property type="entry name" value="SCAI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21243">
        <property type="entry name" value="PROTEIN SCAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12070">
        <property type="entry name" value="SCAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF99398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="597" mass="68707" checksum="8377A5CE6EC0B072" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU71</accession>
    <name>A0AAU9FU71_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oxysterol-binding protein-related protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_07439</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FU71"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13291">
        <property type="entry name" value="PH_ORP10_ORP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF141">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="3" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="14"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF99572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="224" mass="24459" checksum="FEB1BEB181192140" modified="2024-11-27" version="1">MESNLNRMINQTLRHQLSGQLSKYTNVMKGWQYRWFTVDAKTGFLSYYLCDSSAVGDDIAPSPHVLASAPRGQVQLAGAVVYPSDEDSRTFSIACASGDTVKLRANDARARQEWVDGLRAVVESHTKAMDISNSSPLPPRELLAASDAMVSARQALFLTEHNASLARAIESIDCASFTPTDPDLLLLKAISTASTQCLHQCLGLLQRHQEMNQPPAAEALPIML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU81</accession>
    <name>A0AAU9FU81_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_07447</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SARM1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044297">
        <property type="term" value="C:cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003953">
        <property type="term" value="F:NAD+ nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048678">
        <property type="term" value="P:response to axon injury"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09501">
        <property type="entry name" value="SAM_SARM1-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09502">
        <property type="entry name" value="SAM_SARM1-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd24153">
        <property type="entry name" value="SARM1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000056">
        <property type="entry name" value="Ectoderm-expressed 4, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000043">
        <property type="entry name" value="Sterile alpha and TIR motif-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000012">
        <property type="entry name" value="Sterile alpha and TIR motif-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
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    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="NAD(+) HYDROLASE SARM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22998">
        <property type="entry name" value="SARM1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SAM" evidence="13">
        <location>
            <begin position="689"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="12">
        <location>
            <begin position="836"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="339"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1048"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1183"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1261"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1050"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1193"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1288"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1309"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1330"/>
            <end position="1352"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF99587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1395" mass="151651" checksum="CD0C8F7FCEFA18C3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU85</accession>
    <name>A0AAU9FU85_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Probable serine hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_07144</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF99180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SERINE HYDROLASE-LIKE PROTEIN DDB_G0286239"/>
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    </dbReference>
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        <property type="entry name" value="Abhydrolase_1"/>
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    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="59"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF99180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="348" mass="40095" checksum="0277367F50E79B96" modified="2024-11-27" version="1">MDFQEDSSSGTTGNDLGQRYRHAGDLTGEQPTRPFAEISITVPWGHISGKWYGPQNVQPILGLHGWQDNAGTYDLLMPLLSPDVAFLSIDLPGHGLSSRLPDGCYYNSVDNLYVIRLIMKQYKWEKVSLVGHSMSSIICFVFAAVFPDKVDMIIGIDALKPHQRPYPSVIRTMETRLDEFLREDERNRSKNEPPSYTYDELIERVYLGTFHSVNKEHCKHLMARNVQKSEKYPDKFYFCRDRRLKFYNYAIGSQELCVEMAHRIKCPYMFIKATQSSYFEDKKYYDEVLDVLLKKPNFEYIEANGSHHVHMNSPEAIIEPVNNFIQRFGPAAAAAERLKAKEAKESKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FU89</accession>
    <name>A0AAU9FU89_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific protein-tyrosine kinase</fullName>
            <ecNumber evidence="1">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_07498</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="1"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051129">
        <property type="term" value="P:negative regulation of cellular component organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05052">
        <property type="entry name" value="PTKc_Abl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09935">
        <property type="entry name" value="SH2_ABL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11850">
        <property type="entry name" value="SH3_Abl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.330:FF:000014">
        <property type="entry name" value="Abl tyrosine kinase, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000010">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000037">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000004">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000542">
        <property type="entry name" value="Tyrosine-protein kinase Abl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035837">
        <property type="entry name" value="ABL_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015015">
        <property type="entry name" value="F-actin-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08919">
        <property type="entry name" value="F_actin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <reference evidence="25 26" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25 26">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1 20">Membrane</location>
            <topology evidence="1 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16 20">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFG01536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0008331">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009582">
        <property type="term" value="P:detection of abiotic stimulus"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0050906">
        <property type="term" value="P:detection of stimulus involved in sensory perception"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042045">
        <property type="term" value="P:epithelial fluid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016322">
        <property type="term" value="P:neuron remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000007">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
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    <dbReference type="FunFam" id="1.20.120.350:FF:000043">
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        <property type="match status" value="4"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="EF_hand_dom"/>
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        <property type="entry name" value="GPHH_dom"/>
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        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
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        <property type="entry name" value="VDCC_a1su_IQ"/>
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        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002077">
        <property type="entry name" value="VDCCAlpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08763">
        <property type="entry name" value="Ca_chan_IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16905">
        <property type="entry name" value="GPHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00167">
        <property type="entry name" value="CACHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM01062">
        <property type="entry name" value="Ca_chan_IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 18 20" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 20" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4 20" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14 19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="10 20" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="78"/>
            <end position="98"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="257"/>
            <end position="278"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="432"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="462"/>
            <end position="480"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="557"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="633"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="758"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
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            <end position="818"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="827"/>
            <end position="845"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="894"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
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            <end position="1033"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
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            <end position="1107"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1119"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1210"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1299"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="24">
        <location>
            <begin position="1340"/>
            <end position="1375"/>
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    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="381"/>
            <end position="405"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="708"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1500"/>
            <end position="1559"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
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            <end position="1640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1674"/>
            <end position="1744"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1806"/>
            <end position="1834"/>
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    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="660"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="395"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="1576"/>
            <end position="1587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="1591"/>
            <end position="1606"/>
        </location>
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        <location>
            <begin position="1624"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="1685"/>
            <end position="1697"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1721"/>
            <end position="1744"/>
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    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="271"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
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    <feature type="binding site" evidence="18">
        <location>
            <position position="611"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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    <feature type="binding site" evidence="18">
        <location>
            <position position="979"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        <location>
            <position position="235"/>
        </location>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="BFG01536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1834" mass="210443" checksum="93C02E833BB1C21D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5U7</accession>
    <name>A0AAU9G5U7_DROMD</name>
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        <recommendedName>
            <fullName evidence="4 5">NTF2-related export protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="7" type="ORF">DMAD_02468</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Has a role in nuclear-cytoplasmic transport of proteins and mRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9G5U7"/>
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        <property type="component" value="Chromosome E"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000006">
        <property type="entry name" value="NTF2-related export protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045875">
        <property type="entry name" value="NTF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
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        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
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        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NTF2-like"/>
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    </dbReference>
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        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NTF2" evidence="6">
        <location>
            <begin position="15"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00070836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG03141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="135" mass="15549" checksum="2375F5CE6137E826" modified="2024-11-27" version="1">MNSELKTKVESCAHTAEDFTRLYYASFDNRRHQLGRLYLDNAVFSWNGNGANGRQMIERYFLELPSSSHQMNTLDAQPILDAAVGNQLTYLVMASGTVKYHDQPMRNFQQSFIVTAEDDKWKIASDCYRLQEVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5U8</accession>
    <name>A0AAU9G5U8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Beta-alanine transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_02728</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G5U8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS_SLC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="473"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="499"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="83"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG03484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="601" mass="67037" checksum="4C8E322E9C342550" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5V4</accession>
    <name>A0AAU9G5V4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Kinesin-like protein KIF13A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_02593</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22706">
        <property type="entry name" value="FHA_KIF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000002">
        <property type="entry name" value="Kinesin family member 13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000010">
        <property type="entry name" value="Kinesin family member 13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000014">
        <property type="entry name" value="Uncharacterized protein, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
        <property type="entry name" value="Kinesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12473">
        <property type="entry name" value="DUF3694"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
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            <begin position="5"/>
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            <begin position="1497"/>
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    <evidence type="ECO:0000256" key="12">
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03300.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1956" mass="219273" checksum="F991E6EBE7E99C69" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5V6</accession>
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        <submittedName>
            <fullName evidence="15">Kinesin-like protein KIF13A</fullName>
        </submittedName>
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        <name evidence="15" type="ORF">DMAD_02593</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
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    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
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    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
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        <property type="term" value="P:intracellular protein localization"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SMAD/FHA domain"/>
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        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="KINESIN_MOTOR_1"/>
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    </dbReference>
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        <property type="entry name" value="KINESIN_MOTOR_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
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        <location>
            <begin position="1844"/>
            <end position="1886"/>
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            <begin position="1711"/>
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        <ligand>
            <name>ATP</name>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50067"/>
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        <source>
            <dbReference type="PROSITE" id="PS50245"/>
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        <source>
            <dbReference type="EMBL" id="BFG03303.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1935" mass="216885" checksum="BF27B0465604DFD2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5V7</accession>
    <name>A0AAU9G5V7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein kinase C</fullName>
            <ecNumber evidence="2">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_02770</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="2"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G5V7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20794">
        <property type="entry name" value="C1_aPKC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05588">
        <property type="entry name" value="STKc_aPKC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000048">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000070">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000012">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034659">
        <property type="entry name" value="Atypical_PKC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="15">
        <location>
            <begin position="526"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="645"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="16">
        <location>
            <begin position="914"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="193"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="358"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="678"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG03539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="987" mass="108137" checksum="F473562A83346680" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5V8</accession>
    <name>A0AAU9G5V8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial potassium channel ATP-binding subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ATP-binding cassette sub-family B member 8, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Mitochondrial sulfonylurea-receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_02399</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG03052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G5V8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18574">
        <property type="entry name" value="ABC_6TM_ABCB8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000195">
        <property type="entry name" value="AGAP006273-PA-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000403">
        <property type="entry name" value="ATP-binding cassette sub-family B member 8, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF17">
        <property type="entry name" value="MITOCHONDRIAL POTASSIUM CHANNEL ATP-BINDING SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="22" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="281"/>
            <end position="308"/>
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        </location>
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        </location>
    </feature>
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        <location>
            <begin position="720"/>
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        </location>
    </feature>
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        <location>
            <begin position="740"/>
            <end position="750"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00040439"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00041416"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="750" mass="83029" checksum="B7A90E0053B2249B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5V9</accession>
    <name>A0AAU9G5V9_DROMD</name>
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        <submittedName>
            <fullName evidence="3">Larval cuticle protein A3A</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">DMAD_02959</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BFG03827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9G5V9"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CUTICLE PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CHIT_BIND_RR_2"/>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="61"/>
        </location>
    </feature>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG03827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="130" mass="14945" checksum="D04F3E6379606324" modified="2024-11-27" version="1">MQRISPHFQIDQTEEDRLELERQQNESAQYSFNSNIDDQINDGSNSRTETRDGSTVQGSYSYTDGFVKRTVHYIADENGYRVLKDEMQDIGDGPRFNPDGQADVEGSLIGKYSIKLDKSDEEKHYKDIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5W7</accession>
    <name>A0AAU9G5W7_DROMD</name>
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        <submittedName>
            <fullName evidence="9">Solute carrier family 2 facilitated glucose transporter member 1</fullName>
        </submittedName>
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        <name evidence="9" type="ORF">DMAD_03018</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
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    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9 10">RF1</strain>
        </source>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="6">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF127">
        <property type="entry name" value="FI08437P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="437"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="21"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG03893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="485" mass="52438" checksum="50DF5CBEDFF9D70D" modified="2024-11-27" version="1">MGSTKNNNDKPGWSKLLLLVAFATTFGGAVSTGYCIGVINAPSKLMKIWCNQTLHDNYGSNLSETGLDLLWSSIVSVFLVGGAIGSLGGAGAANKFGRKACYLICGALFSVGAVLFFFCRAANSVEMLLIGRFIVGLASGLVTATLPMYLSEVAPLALRGTLGVFCAVGVTGGVVVGQVCSLADVFGTADLWHYALSAYMGLIIVCYLPSYLFPESPKFLYIVKGNRAAAKRELQRLRGEDAEELIAQELAEMEAEANAKVQTSSFCDVLRDSRLLLPLVIVCCFHGGQQLSGINAIFYYSVSIFEKAGLSTVNAQWANLGAGCLNLAISLLGPWLMAYCNRRTLMMFSCAMCSVFLFTIAFVLYYIDFVSWFAIACICCIMGYIFFYQFGLGPIPYFIGSELFEVAPRSVAMSMGSLASWTCNFIIGISFPSLQNAWGAFVFLPFSITCVLLCLLTKFYLPETRGRDPSEVAPLVSNGFRSKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5X3</accession>
    <name>A0AAU9G5X3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sprouty-related EVH1 domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_02971</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G5X3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10574">
        <property type="entry name" value="EVH1_SPRED-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <end position="120"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
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    <sequence length="399" mass="43821" checksum="9C2A0DDB447B5486" modified="2024-11-27" version="1">MTEGHESEFLVTVRAQVMTRDESTEGWLPLAGGGLANVSIRKRARLSPLASGHDYIIYGQRISDQSVILSCVINRDLKYYKVMPTFHHWRAGKQRNGLTFQTAADARAFDKGVLRAYNELIDDDVFMTLDLPVESESLQKIHSSPEGSEKSHKSKDSYSNNSDSEKLPAAPIHYISTDKTSTTTTAQDGPPSSTAPAPGAAQIAASENYSYVTLTAVHHDYNYPVVDQPVGAQVLNARRESISALKKRNALEAAQAMAAAQTAGTAGSGKALQKQNVSDILKKETRLRCRYCHELYSEEFNRRGACEYAPDTFRTGYECISGMGCARCMIYHCMSDAEGETAQHPCDCSVTEAGCTKRWLGLAVLSLFVPCLWCYPPLRACHWMGIQCGLCGAPHKPHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5X7</accession>
    <name>A0AAU9G5X7_DROMD</name>
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            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">DMAD_02292</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">RF1</strain>
        </source>
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        <property type="component" value="Chromosome A"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFG02919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="126" mass="14059" checksum="87326E42D58EA817" modified="2024-11-27" version="1">MLVLNSSERQVLNKREPLVPSKMGLQGLNILEMLVRNNLELLALNKTVPQAPSSWTANCRLRARYMLEPECPKTIVRGVHRTRCRGRASPRSPACSQLRPGPAGTRLPVVKGEAIRTCCSRVDLKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G5X8</accession>
    <name>A0AAU9G5X8_DROMD</name>
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        <recommendedName>
            <fullName evidence="12">Diacylglycerol kinase</fullName>
            <shortName evidence="12">DAG kinase</shortName>
            <ecNumber evidence="12">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_02752</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
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        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome E"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20851">
        <property type="entry name" value="C1_DGK_typeI_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20845">
        <property type="entry name" value="C1_DGKbeta_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000017">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000003">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.110">
        <property type="entry name" value="Diacylglycerol kinase alpha"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047471">
        <property type="entry name" value="C1_DGKbeta-like_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029477">
        <property type="entry name" value="DAG_kinase_typeI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038199">
        <property type="entry name" value="DGK_typeI_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF48">
        <property type="entry name" value="DIACYLGLYCEROL KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14513">
        <property type="entry name" value="DAG_kinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 12 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="283"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="15">
        <location>
            <begin position="379"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="15">
        <location>
            <begin position="446"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="DAGKc" evidence="16">
        <location>
            <begin position="537"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="172"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="752"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="944"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="865"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="209"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="766"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="797"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="835"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="951"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="981"/>
            <end position="997"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFG03514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1246" mass="136920" checksum="ECE991AF06EFC454" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G686</accession>
    <name>A0AAU9G686_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Grh/CP2 DB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_03084</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the grh/CP2 family. CP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G686"/>
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        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="SAM_CP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gemini, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007604">
        <property type="entry name" value="CP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041418">
        <property type="entry name" value="SAM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040167">
        <property type="entry name" value="TF_CP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037:SF21">
        <property type="entry name" value="GEMINI, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037">
        <property type="entry name" value="TRANSCRIPTION FACTOR CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04516">
        <property type="entry name" value="CP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18016">
        <property type="entry name" value="SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51968">
        <property type="entry name" value="GRH_CP2_DB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Grh/CP2 DB" evidence="4">
        <location>
            <begin position="398"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="27"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="89"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="197"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="272"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="350"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="553"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="623"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="154"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
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            <end position="377"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="553"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="623"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFG03998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="949" mass="104742" checksum="E3BDA53EE3532E64" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G687</accession>
    <name>A0AAU9G687_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nuclear pore complex protein Nup75</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Nucleoporin Nup85</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_02692</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the nucleoporin Nup85 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G687"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011502">
        <property type="entry name" value="Nucleoporin_Nup85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373">
        <property type="entry name" value="FROUNT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373:SF21">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07575">
        <property type="entry name" value="Nucleopor_Nup85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7 9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG03435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="666" mass="76829" checksum="1029160305CFCE28" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G688</accession>
    <name>A0AAU9G688_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitric oxide-associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_02694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G688"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01855">
        <property type="entry name" value="YqeH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052807">
        <property type="entry name" value="Mito_transl_resp_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46406">
        <property type="entry name" value="NITRIC OXIDE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46406:SF1">
        <property type="entry name" value="NITRIC OXIDE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="263"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG03438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="611" mass="69008" checksum="3C7B1A72A77DE70E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G689</accession>
    <name>A0AAU9G689_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_02871</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 12">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G689"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF300">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="122"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG03674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="285" mass="32336" checksum="7F90545A8389AA97" modified="2024-11-27" version="1">MSSTALSTDVGNIDQLILRIIDVRRIKQLHLSETEIRGICLRSRDVFLSQPMLLELSAPVKICGDIHGQFTDLLRLFDYGGYPPSSNYLFLGDYVDRGKQSIETLCLLLAYKIKYPENFFLLRGNHESAGINRIYGFFDECKRRYTVKLWRTFVDCYNCMPVAASVDEKIFCCHGGLSPDLLNMNQISQLNRPCDVPDKGLLCDLLWSDPDAKIMGWGENDRGVVEDGYEFFAKRQLITVFSAPNYCGEFDNAGAMMSVDETLMCSFYVLKPPKKAGMRKVHSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G698</accession>
    <name>A0AAU9G698_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Nephrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_02734</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G698"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000835">
        <property type="entry name" value="Hibris, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001208">
        <property type="entry name" value="Hibris, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001241">
        <property type="entry name" value="Hibris, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001273">
        <property type="entry name" value="Hibris, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002651">
        <property type="entry name" value="Hibris, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000405">
        <property type="entry name" value="nephrin isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF136">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043728712" evidence="9">
        <location>
            <begin position="19"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1050"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="41"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="146"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="256"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="351"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="453"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="548"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="649"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="744"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="838"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="942"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1152"/>
            <end position="1173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG03493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GB51</accession>
    <name>A0AAU9GB51_DROMD</name>
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        <recommendedName>
            <fullName evidence="6">Protein lingerer</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="ORF">DMAD_03952</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8 9">RF1</strain>
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        <text evidence="5">Acts in the nervous system to mediate the control of copulatory organs during courtship.</text>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00056974"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00074733"/>
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            <dbReference type="EMBL" id="BFG05145.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1315" mass="131744" checksum="FC55E2882DFCE69D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GB56</accession>
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        </recommendedName>
    </protein>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
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        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
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        </subcellularLocation>
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        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
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    </dbReference>
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    </dbReference>
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        <source>
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        <source>
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        </source>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="428" mass="47988" checksum="05D0DED31E920B3A" modified="2024-11-27" version="1">MEISEPSIGIFYISKLLALAPYATRKNSKGQYEIRRSWLFSIYSASLTVAMVFLTYRGLLFDANAKIPVRMKSATSKVVTALDVSVVVLAVVSGVYCGLLSLSETLELNARLYRIDNTLNACNHSRRDRWRALGMAGVSLLTISALLGLDVGTWMHKAEEMNISDSDTELNVHWYIPFYSLYFILTGLHINFANTAYGLGRRFRRLNQMLSSSFLGEPKECNDALKLRKMSTVKTVSTNATMTPTALHSSLTKLSSEMLPNESAAKNKSLLIQTMADNHDSLTKCVLLLSNSFGIAVLFILVSCLLHLVATAYFLFLELLSKRDDAYMWVQALWILLHSFRLLMVVEPCHLAARESRKTIQIVCEIERKVHEPILAEAVKKFWQQLLVVDSDFSASGLCRVNRNILTSFASAIATYLVILIQFQKTNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFW0</accession>
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        <submittedName>
            <fullName evidence="13">Protein enabled</fullName>
        </submittedName>
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        <name evidence="13" type="ORF">DMAD_05037</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ena/VASP family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GFW0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01207">
        <property type="entry name" value="EVH1_Ena_VASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000011">
        <property type="entry name" value="protein enabled isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000047">
        <property type="entry name" value="vasodilator-stimulated phosphoprotein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038023">
        <property type="entry name" value="VASP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014885">
        <property type="entry name" value="VASP_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202:SF22">
        <property type="entry name" value="PROTEIN ENABLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08776">
        <property type="entry name" value="VASP_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118370">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein, VASP, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0729">SH3-binding</keyword>
    <feature type="domain" description="RanBD1" evidence="11">
        <location>
            <begin position="136"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="WH1" evidence="12">
        <location>
            <begin position="153"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="316"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="440"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="320"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="381"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="455"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="498"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="520"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="543"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="582"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="634"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="722"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="750"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG06551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="847" mass="90877" checksum="0659CCA69B2AF38B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFW4</accession>
    <name>A0AAU9GFW4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14114">
        <property type="entry name" value="STKc_Twitchin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000321">
        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000440">
        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000097">
        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000642">
        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000614">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001845">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001892">
        <property type="entry name" value="Bent, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000460">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000504">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000505">
        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000147">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002083">
        <property type="entry name" value="Protein CBR-UNC-22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000160">
        <property type="entry name" value="Titin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000003">
        <property type="entry name" value="Titin isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000012">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000127">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001003">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000249">
        <property type="entry name" value="twitchin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000056">
        <property type="entry name" value="twitchin isoform X4"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000936">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000006">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000775">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000831">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001034">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001164">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000567">
        <property type="entry name" value="Uncharacterized protein, isoform G"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002180">
        <property type="entry name" value="Uncharacterized protein, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000466">
        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000876">
        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001033">
        <property type="entry name" value="Uncharacterized protein, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000935">
        <property type="entry name" value="Uncharacterized protein, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001001">
        <property type="entry name" value="Uncharacterized protein, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000051">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000553">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000559">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG06697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="8397" mass="938189" checksum="3588D69536B730F0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFW9</accession>
    <name>A0AAU9GFW9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14114">
        <property type="entry name" value="STKc_Twitchin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform C"/>
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    </dbReference>
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        <property type="entry name" value="Bent, isoform F"/>
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        <property type="entry name" value="Bent, isoform J"/>
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        <property type="entry name" value="Bent, isoform J"/>
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        <property type="entry name" value="Bent, isoform J"/>
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        <property type="entry name" value="Myosin light chain kinase"/>
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        <property type="entry name" value="Myosin-binding protein C, slow type"/>
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        <property type="entry name" value="Protein CBR-UNC-22"/>
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        <property type="entry name" value="Titin a"/>
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        <property type="entry name" value="titin isoform X1"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="28"/>
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    </dbReference>
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    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0514">Muscle protein</keyword>
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    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG06712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="8218" mass="918532" checksum="1F867B219F1A3B24" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFY0</accession>
    <name>A0AAU9GFY0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14114">
        <property type="entry name" value="STKc_Twitchin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform F"/>
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        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Bent, isoform H"/>
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        <property type="entry name" value="Bent, isoform H"/>
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        <property type="entry name" value="Bent, isoform J"/>
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        <property type="entry name" value="Bent, isoform J"/>
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        <property type="entry name" value="Bent, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myosin light chain kinase"/>
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        <property type="entry name" value="Myosin-binding protein C, slow type"/>
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        <property type="entry name" value="Protein CBR-UNC-22"/>
        <property type="match status" value="4"/>
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        <property type="entry name" value="Titin a"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Titin isoform E"/>
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        <property type="entry name" value="titin isoform X1"/>
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        <property type="entry name" value="titin isoform X1"/>
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        <property type="entry name" value="titin isoform X1"/>
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        <property type="entry name" value="twitchin isoform X2"/>
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        <property type="entry name" value="twitchin isoform X4"/>
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        <property type="entry name" value="Uncharacterized protein, isoform H"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform I"/>
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        <property type="entry name" value="Uncharacterized protein, isoform I"/>
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    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="78"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
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    </dbReference>
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        <property type="entry name" value="FN3_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
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        <property type="match status" value="39"/>
    </dbReference>
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        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="40"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="38"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
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        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
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        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="9"/>
            <end position="102"/>
        </location>
    </feature>
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    <feature type="domain" description="Ig-like" evidence="14">
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    <feature type="domain" description="Ig-like" evidence="14">
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        <location>
            <begin position="1826"/>
            <end position="1910"/>
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        <location>
            <begin position="2104"/>
            <end position="2201"/>
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    </feature>
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        <location>
            <begin position="2204"/>
            <end position="2302"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="2306"/>
            <end position="2396"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="2403"/>
            <end position="2499"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="2505"/>
            <end position="2600"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="2604"/>
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        </location>
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    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="2702"/>
            <end position="2798"/>
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    </feature>
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        <location>
            <begin position="2805"/>
            <end position="2900"/>
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            <begin position="2904"/>
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    <feature type="domain" description="Fibronectin type-III" evidence="15">
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            <begin position="3000"/>
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            <begin position="3105"/>
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            <begin position="3204"/>
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        <location>
            <begin position="3301"/>
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    <feature type="domain" description="Fibronectin type-III" evidence="15">
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            <begin position="3402"/>
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        </location>
    </feature>
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        <location>
            <begin position="3501"/>
            <end position="3593"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="3601"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <dbReference type="EMBL" id="BFG06560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="876" mass="95003" checksum="BA1B15682BDA6EE0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFY7</accession>
    <name>A0AAU9GFY7_DROMD</name>
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        <submittedName>
            <fullName evidence="3">Slo-interacting protein 1</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">DMAD_13647</name>
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    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BFG06727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SLO-INTERACTING PROTEIN 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PDZ"/>
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    </dbReference>
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        <location>
            <begin position="194"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="354"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG06727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="731" mass="81766" checksum="451B64A57E88980D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFZ2</accession>
    <name>A0AAU9GFZ2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mucin-5AC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_05001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG06504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GFZ2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043538191" evidence="2">
        <location>
            <begin position="32"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="603"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="481"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="493"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="662"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="714"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="784"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="796"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG06504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="829" mass="87494" checksum="21894A048A3FD0A2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFZ5</accession>
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        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
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        <name evidence="16" type="ORF">DMAD_13643</name>
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    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000642">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000504">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein CBR-UNC-22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000160">
        <property type="entry name" value="Titin a"/>
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    </dbReference>
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        <property type="entry name" value="Titin isoform E"/>
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    </dbReference>
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        <property type="entry name" value="titin isoform X1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001001">
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    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000553">
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000559">
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000748">
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001016">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
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        </source>
    </evidence>
    <sequence length="8068" mass="901547" checksum="0E4F563039E724D9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GFZ7</accession>
    <name>A0AAU9GFZ7_DROMD</name>
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            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26 27">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome dot"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="EphR_LBD"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PTKc_EphR"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
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    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Transcription Factor, Ets-1"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
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    </dbReference>
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        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="SAM"/>
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        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
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        <property type="entry name" value="Tyr_kinase_ephrin_rcpt"/>
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        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877:SF14">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00790">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_1"/>
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    </dbReference>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 18 20" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="15 26" id="KW-0675">Receptor</keyword>
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    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="648"/>
            <end position="671"/>
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        <location>
            <begin position="92"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="398"/>
            <end position="505"/>
        </location>
    </feature>
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        <location>
            <begin position="542"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="740"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="23">
        <location>
            <begin position="1034"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="867"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="746"/>
            <end position="754"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18 20">
        <location>
            <position position="774"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <begin position="141"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-3"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
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    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="BFG06742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1095" mass="123209" checksum="DDCA5C10DBF79616" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GG43</accession>
    <name>A0AAU9GG43_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase C-like 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_05038</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Yamamoto D."/>
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            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1005"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1150"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1169"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1333"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1390"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1681"/>
            <end position="1692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1847"/>
            <end position="1857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1871"/>
            <end position="1884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1895"/>
            <end position="1910"/>
        </location>
    </feature>
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        <location>
            <begin position="1933"/>
            <end position="1944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1999"/>
            <end position="2023"/>
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        <location>
            <begin position="2030"/>
            <end position="2046"/>
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        <location>
            <begin position="2069"/>
            <end position="2087"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
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    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
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        <source>
            <dbReference type="PROSITE" id="PS51258"/>
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    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG06680.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3326" mass="373547" checksum="8DBD068200FFF12C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GG91</accession>
    <name>A0AAU9GG91_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Twitchin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_13643</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029268">
        <property type="protein sequence ID" value="BFG06695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome dot"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Ig_Titin_like"/>
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    </dbReference>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="40"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="38"/>
    </dbReference>
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        <property type="entry name" value="IGc2"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
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        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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        <property type="entry name" value="FN3"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="9"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="119"/>
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        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="228"/>
            <end position="321"/>
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    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="335"/>
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    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="438"/>
            <end position="531"/>
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    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="543"/>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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        <location>
            <begin position="650"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
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            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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            <end position="1036"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1394"/>
            <end position="1462"/>
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        <location>
            <begin position="1"/>
            <end position="27"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="181"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="421"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="453"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="478"/>
            <end position="491"/>
        </location>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="800"/>
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            <begin position="945"/>
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        <location>
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        <location>
            <begin position="1443"/>
            <end position="1462"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009200"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFG06761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1530" mass="157809" checksum="2AACB8154F7CD1E1" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EUA4</accession>
    <name>A0AAU9EUA4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA replication licensing factor REC</fullName>
        </submittedName>
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        <name evidence="6" type="ORF">DMAD_08701</name>
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    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
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        <text evidence="3">Belongs to the MCM family.</text>
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        <property type="protein sequence ID" value="BFF90115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="NA-bd_OB-fold"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="DNA HELICASE MCM8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF90115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="751" mass="82808" checksum="EBB6F819FCA394AC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6A6</accession>
    <name>A0AAU9F6A6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL9m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L9, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_09301</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F6A6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="39S ribosomal protein L9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="entry name" value="Ribosomal protein L9, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_bL9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_bL9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_bL9_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Ribosomal protein L9" evidence="9">
        <location>
            <begin position="68"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF90856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="248" mass="28681" checksum="DF8257574F9F38BD" modified="2024-11-27" version="1">MLKNICVTQLNLLKSASSLQQQVRTTFILKRKYEPLLHKTNERPRPMRPKHFIYELVEDTQVKKRPNMEVVLKTFVEGVGDKGDVVSMKPHFVYNKLLLPGLAAYKTPENIAKYAKTDAEKSAVKHSSAYAQRTVNMLETIVLAVVMNKDEPWVLEPWHIKASLRKAGFHCKEECITLPKERIEGPDLKKESKDFQCIITINKLEQATLKCRLHHWSTDPSERLPYVLEHWKMQSEPLLDVGSPEKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6E1</accession>
    <name>A0AAU9F6E1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Synapse-associated protein of 47 kDa</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_08415</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F6E1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048172">
        <property type="term" value="P:regulation of short-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3970.10:FF:000001">
        <property type="entry name" value="synapse-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051494">
        <property type="entry name" value="BSD_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019:SF6">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD" evidence="3">
        <location>
            <begin position="275"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="345"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="463"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="495"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="507"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF89723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="522" mass="54105" checksum="82A812C7EDAAE379" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6E2</accession>
    <name>A0AAU9F6E2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Signal peptide peptidase-like 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_07714</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A22B family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F6E2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030660">
        <property type="term" value="C:Golgi-associated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF22">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF88797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="428" mass="46296" checksum="810711A7CB2D2C1E" modified="2024-11-27" version="1">MSLGSVGGSGGSLGAQTVGAGSIGGGAAAAAAAAAAGGSNGEYRELHWTVSSVMDSSRVSTCLISMLLIVYGSFRSLNIEQEAREREQKKRNESTTNLLTGEHVEKEPTDKFATLDTMHALCLPLGASISLLIMFFFFDSMQLLFAVCTAIIATVALAFLLLPMCQYIMRPCTDGKRFSFGVCGRFTGAELFSFMLSVSIVCVWVLTGHWLLMDAMGMGLCVAFIAFVRLPSLKVSTLLLTGLLIYDVFWVFLSSYIFSTNVMVKVATRPAENPVGIVARKLNLGGIVRDTPKLNLPGKLVFPSIHNTGHFSMLGLGDVVMPGLLLCFVLRYDAYKKSQGVTSDPTLSTPRGVGSRLTYFHCSLLGYFLGLLTATVSSEVFKAAQPALLYLVPFTLLPLLLMAYLKGDLRRMWSEPFITHPPSKQLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6E6</accession>
    <name>A0AAU9F6E6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_08425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F6E6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015897">
        <property type="entry name" value="CHK_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF6">
        <property type="entry name" value="CHK DOMAIN OV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="130"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF89738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="415" mass="47540" checksum="26DDC4B29FA9781F" modified="2024-11-27" version="1">MAAIKVPEWINAELFEDVLKSTVPGYTKVKTFKADIGSAAGENYATIMLRVNIEVELEDGKSKDVSYMLKLPHQLELYQEMMKQNNIFDIERMMYSEVVPEMEALYKEVGVDVIFGARSYDFKGAKTDYVLLEDLGQKGFKNANRLEGLDQTHTERVLKKLSQWHAASAVRVATKGAYPEILNLGFLKEESRPMMTDMIKGMMARFLKVCVTYEGHEEYIEQIKNLIPVTVDEMYKMAKIDAQEFNVLNHGDFWSNNVMFQYDAFGKIKEVYLVDYQIPKYGTVAQDLLYFLLSSTKLEDKLSKFDYYIKFYHDNLIEHLKILKYTKPLPTLRSIHSSLLKHGVFGYSVVTGVMGAVLLDPSENASFENFVGNSEAGEAFQMQLYTNPRYRKHIQVILPWLLNRGALETSAPTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6E7</accession>
    <name>A0AAU9F6E7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Very low-density lipoprotein receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_07725</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F6E7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
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        <source>
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        <source>
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    <sequence length="864" mass="96193" checksum="0096A82B057E02BF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10 11">RF1</strain>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF94723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="201" mass="20836" checksum="8789A8BF29E573F2" modified="2024-11-27" version="1">MVDASIKSLKERGGSSLLAIKKYITAEYKCDAQKLAPFIKKYLKSAVANGKLIQTKGKGASGSFKLSASAKKEPKPKVASVEKKVKTKKVVSSAAAKKTASPKKAAGAGDKKLKAKKAVATKKTAEKKKTEKAKAKEVKKTGTVKAKPAAAKVAKSSPAKPKPKAKAVAAATSAKPKKTTKKPAAPATVKKPKAKTTAAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FGA5</accession>
    <name>A0AAU9FGA5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Synaptotagmin 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_12367</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Synaptic vesicle membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FGA5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031045">
        <property type="term" value="C:dense core granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007626">
        <property type="term" value="P:locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08385">
        <property type="entry name" value="C2A_Synaptotagmin-1-5-6-9-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08402">
        <property type="entry name" value="C2B_Synaptotagmin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000007">
        <property type="entry name" value="Synaptotagmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000016">
        <property type="entry name" value="Synaptotagmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF227">
        <property type="entry name" value="SYNAPTOTAGMIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="188"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="321"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="53"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="169"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF94841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="470" mass="52870" checksum="1D5A941B55FD85A9" modified="2024-11-27" version="1">MPPNAKSEADAKPDEEPAPESATQLESVDQKLEETHHSRFRELDKQEQESAAEEAAAAESQRIAQVETTTRSATTEAQESTTTSLPVIKKIEHAGEVVTEAIAERTGLPTWGVVAIIILVFLVVFGIIFFCVRRFLKKRRTKDGKGKKGVDMKSVQLLGSAYKEKPDMEELTENAEEGDEEDKQSEQKLGRLNFKLEYDFNSNSLAVTVIQAEELPALDMGGTSDPYVKVYLLPDKKKKFETKVHRKTLSPVFNETFTFKSLPYADAMNKTLVFAIFDFDRFSKHDQIGEVKVPLCTIDLAQTIEEWRDLVSVEGEGGQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIAIMQNGKRLKKKKTSIKKCTLNPYYNESFSFEVPFEQIQKICLVVTVVDYDRIGTSEPIGRCILGCMGTGTELRHWSDMLASPRRPIAQWHTLKDPEETDEILKNMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FGA7</accession>
    <name>A0AAU9FGA7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Protein split ends</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_12256</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM Spen family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12349">
        <property type="entry name" value="RRM2_SHARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12350">
        <property type="entry name" value="RRM3_SHARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12351">
        <property type="entry name" value="RRM4_SHARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21543">
        <property type="entry name" value="SPOC_SHARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000088">
        <property type="entry name" value="msx2-interacting protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000118">
        <property type="entry name" value="msx2-interacting protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.290.10:FF:000002">
        <property type="entry name" value="Spen family transcriptional repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000258">
        <property type="entry name" value="Split ends, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034173">
        <property type="entry name" value="SHARP_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034174">
        <property type="entry name" value="SHARP_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034175">
        <property type="entry name" value="SHARP_RRM4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010912">
        <property type="entry name" value="SPOC_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50917">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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    <sequence length="2568" mass="290760" checksum="C68EBD8AC8EA1B17" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="17">Piezo-type mechanosensitive ion channel component</fullName>
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        <name evidence="17" type="ORF">DMAD_12317</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF15917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF24871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF24874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF94780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2556" mass="289496" checksum="EC4A51BAAC59D193" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FGF5</accession>
    <name>A0AAU9FGF5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Piezo-type mechanosensitive ion channel component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_12317</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PIEZO (TC 1.A.75) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027272">
        <property type="entry name" value="Piezo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031334">
        <property type="entry name" value="Piezo_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056770">
        <property type="entry name" value="Piezo_THU9_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056769">
        <property type="entry name" value="Piezo_TM1-24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031805">
        <property type="entry name" value="Piezo_TM25-28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056768">
        <property type="entry name" value="THU_Piezo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47049">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47049:SF2">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12166">
        <property type="entry name" value="Piezo_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24874">
        <property type="entry name" value="Piezo_THU9_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24871">
        <property type="entry name" value="Piezo_TM1-24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15917">
        <property type="entry name" value="Piezo_TM25-28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23188">
        <property type="entry name" value="THU_Piezo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="59"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="401"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="459"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="516"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="546"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="586"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="611"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="639"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="692"/>
            <end position="712"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="818"/>
            <end position="843"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="855"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1001"/>
            <end position="1017"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1024"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1074"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1149"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1176"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1209"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1277"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1610"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1642"/>
            <end position="1661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1668"/>
            <end position="1692"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1717"/>
            <end position="1735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1836"/>
            <end position="1858"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1878"/>
            <end position="1899"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1911"/>
            <end position="1930"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1942"/>
            <end position="1959"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2051"/>
            <end position="2074"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2343"/>
            <end position="2366"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo TM1-24" evidence="15">
        <location>
            <begin position="26"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo TM25-28" evidence="13">
        <location>
            <begin position="1134"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo transmembrane helical unit" evidence="14">
        <location>
            <begin position="1624"/>
            <end position="1742"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo THU9 and anchor" evidence="16">
        <location>
            <begin position="1834"/>
            <end position="2073"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo non-specific cation channel cap" evidence="12">
        <location>
            <begin position="2111"/>
            <end position="2430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="729"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1428"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2221"/>
            <end position="2245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2436"/>
            <end position="2462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1432"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1497"/>
            <end position="1511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="2226"/>
            <end position="2245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF15917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF24871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF24874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF94783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2462" mass="279705" checksum="959D6BE20A47C6A4" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FGF7</accession>
    <name>A0AAU9FGF7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 6">Acylphosphatase</fullName>
            <ecNumber evidence="2 5 6">3.6.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_12418</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 5 6">
            <text>an acyl phosphate + H2O = a carboxylate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the acylphosphatase family.</text>
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    <dbReference type="EC" id="3.6.1.7" evidence="2 5 6"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FGF7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000011">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10029">
        <property type="entry name" value="ACYLPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10029:SF3">
        <property type="entry name" value="ACYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00112">
        <property type="entry name" value="ACYLPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="Acylphosphatase/BLUF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00151">
        <property type="entry name" value="ACYLPHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="9">
        <location>
            <begin position="36"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF94901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="126" mass="14267" checksum="F6BB3CACD541C4AF" modified="2024-11-27" version="1">MNNKITKSKFTPTVTPPLKRKTAAAKKRTDEENIFGCKFEVFGKVQGVYFRKHTNNKAKRLGLVGWCMNTDEGSVKGVMEGSLENIIEMKNWLQHKGSPRSVIQKAVFSANEPLVSSNFETFSIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FP19</accession>
    <name>A0AAU9FP19_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Vesicle-associated membrane protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_05709</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Synaptic vesicle membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FP19"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15870">
        <property type="entry name" value="R-SNARE_VAMP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000013">
        <property type="entry name" value="Vesicle-associated membrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="49"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="143"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF97261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="189" mass="19705" checksum="8D06501B3F32B650" modified="2024-11-27" version="1">MGKKDKNKEPADAAPAGDAPPAGPPAGEGGDGEIVGGPHNPQQIAAQKRLQQTQAQVDEVVDIMRTNVEKVLERDSKLSELDDRADALQQGASQFEQQAGKLKRKFWLQNLKMMIIMGVIGLVVVGIIANKLGLIGGEQPPQYPQYPQYMPPPPPQQQQPAAAGGQSALVDGGHGTGAGAGANPEHGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FP25</accession>
    <name>A0AAU9FP25_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Polycomb protein PHO</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_05809</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FP25"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000104">
        <property type="entry name" value="Transcriptional repressor protein YY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000163">
        <property type="entry name" value="transcriptional repressor protein YY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF19">
        <property type="entry name" value="YY2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="537"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="566"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="594"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="624"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="451"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="649"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="451"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="660"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF97380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="710" mass="77694" checksum="03DAB7594FD9F8F9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FP29</accession>
    <name>A0AAU9FP29_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein alan shepard</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_05858</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
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        <text evidence="3">Has a role in the perception of gravity.</text>
    </comment>
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        <property type="entry name" value="protein alan shepard isoform X4"/>
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        <property type="entry name" value="Uncharacterized protein, isoform D"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
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    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
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        <property type="entry name" value="RRM"/>
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    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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            <begin position="21"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="109"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="355"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFF97447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="381" mass="41580" checksum="A05C7857CBE39C34" modified="2024-11-27" version="1">MGPNGTVQNQNQQGGEQLSKTNLYIRGLQQGTTDKDLVNMCAQYGTIISTKAILDKTTNKCYGFVDFEQPAFAECAVKGLQGKGVQAQMAKVGIWVLHRPAIQQEQDPTNLYIANLPPHFKETDLESMLSKYGQVVSTRILRDQQMNSKGVGFARMESREKCEQIIQMFNGNTIPGAKDPLLVKFADGGPKKKNLFKTPDPNARAWRDVSAEGIPVTYDPSMQQNGVSVNVGTPIGVPYSRFGAPQVGGYPVAGSQWIPGYMMTQPITQVDDQYMQMAAAPQLGVTSYKPEAVNQVQPRGISMMVSGDTAVPYGTMMPQLATLQIGNSNFSPSLQYISPTYPYYAPPTIIPTMPMTDSEQASTAASPDEAYTQYPHQAAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FP32</accession>
    <name>A0AAU9FP32_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_05849</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 13 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009401">
        <property type="entry name" value="Med13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051139">
        <property type="entry name" value="Mediator_complx_sub13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021643">
        <property type="entry name" value="Mediator_Med13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041285">
        <property type="entry name" value="MID_MedPIWI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48249">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48249:SF3">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06333">
        <property type="entry name" value="Med13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11597">
        <property type="entry name" value="Med13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18296">
        <property type="entry name" value="MID_MedPIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0010">Activator</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med13 N-terminal" evidence="12">
        <location>
            <begin position="12"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="MID" evidence="13">
        <location>
            <begin position="1882"/>
            <end position="2174"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit Med13 C-terminal" evidence="11">
        <location>
            <begin position="2212"/>
            <end position="2616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="240"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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            <end position="511"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="10">
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="10">
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        </location>
    </feature>
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            <begin position="1983"/>
            <end position="2063"/>
        </location>
    </feature>
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        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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    </feature>
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        </location>
    </feature>
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            <begin position="920"/>
            <end position="931"/>
        </location>
    </feature>
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        <location>
            <begin position="1185"/>
            <end position="1227"/>
        </location>
    </feature>
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        <location>
            <begin position="1245"/>
            <end position="1255"/>
        </location>
    </feature>
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        <location>
            <begin position="1257"/>
            <end position="1273"/>
        </location>
    </feature>
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        <location>
            <begin position="1343"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
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            <end position="1379"/>
        </location>
    </feature>
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            <end position="1538"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="2039"/>
            <end position="2053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF11597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF18296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF97434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2627" mass="280910" checksum="ABC64E227BA1B3CF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FP38</accession>
    <name>A0AAU9FP38_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DMAD_05822</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FP38"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF97395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="88" mass="10473" checksum="631CBD5AA64853DF" modified="2024-11-27" version="1">MARYRTIMCQTRISNHSLIPRDTQKNLPNVEAISNVKTSLGYRWPLAAPEQRIFHKNTMQKLNRHMILYCTMLASVKTDSERQRKRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FP39</accession>
    <name>A0AAU9FP39_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ATP-binding cassette sub-family G member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_05760</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FP39"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF105">
        <property type="entry name" value="FI02074P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="367"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="403"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="516"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="3"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="9">
        <location>
            <begin position="272"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF97323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="544" mass="61351" checksum="B073B987802A441C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FP42</accession>
    <name>A0AAU9FP42_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prominin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_05869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FP42"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043930681" evidence="1">
        <location>
            <begin position="32"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF97462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="141" mass="15738" checksum="F1199C2A8DF72965" modified="2024-11-27" version="1">MTPPVPHRRTKRDHGPLYVLVLASLLMLALAQVPDGNVEYDPRGPRAATTQALANDTGTGTGTPSWWRAGYEGDGTTHEQLGQLHWPPVVYTRFEGHPNYSRDLSSPTKSVDFIYNFTHSLFELVFTDDPVLPQSEYQIAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FP74</accession>
    <name>A0AAU9FP74_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large proline-rich protein BAG6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">BCL2-associated athanogene 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">HLA-B-associated transcript 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">DMAD_06122</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25 26">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2).</text>
    </comment>
    <comment type="function">
        <text evidence="1">Released extracellularly via exosomes, it is a ligand of the natural killer/NK cells receptor NCR3 and stimulates NK cells cytotoxicity. It may thereby trigger NK cells cytotoxicity against neighboring tumor cells and immature myeloid dendritic cells (DC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Component of the BAG6/BAT3 complex, also named BAT3 complex, at least composed of BAG6, UBL4A and GET4/TRC35. Interacts with GET4; the interaction is direct and localizes BAG6 in the cytosol. Interacts with UBL4A; the interaction is direct and required for UBL4A protein stability. Interacts with AIFM1. Interacts with HSPA2. Interacts with CTCFL. Interacts with p300/EP300. Interacts (via ubiquitin-like domain) with RNF126; required for BAG6-dependent ubiquitination of proteins mislocalized to the cytosol. Interacts (via ubiquitin-like domain) with SGTA; SGTA competes with RNF126 by binding the same region of BAG6, thereby promoting deubiquitination of BAG6-target proteins and rescuing them from degradation. Interacts with ricin A chain. Interacts with VCP and AMFR; both form the VCP/p97-AMFR/gp78 complex. Interacts with SYVN1. Interacts with USP13; the interaction is direct and may mediate UBL4A deubiquitination. Interacts with ZFAND2B. Interacts with KPNA2. Interacts with UBQLN4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular exosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FP74"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071818">
        <property type="term" value="C:BAT3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01809">
        <property type="entry name" value="Ubl_BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000161">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021925">
        <property type="entry name" value="BAG6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15204">
        <property type="entry name" value="LARGE PROLINE-RICH PROTEIN BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15204:SF0">
        <property type="entry name" value="LARGE PROLINE-RICH PROTEIN BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12057">
        <property type="entry name" value="BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="17" id="KW-0143">Chaperone</keyword>
    <keyword evidence="13" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0221">Differentiation</keyword>
    <keyword evidence="14" id="KW-0391">Immunity</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="15" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="24">
        <location>
            <begin position="3"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="76"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="283"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="607"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="675"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="766"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="857"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1083"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="312"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="608"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
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    <sequence length="1250" mass="131813" checksum="5769ADAAFE2DFFCF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FP80</accession>
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        <name evidence="3" type="ORF">DMAD_05783</name>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">RF1</strain>
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</entry>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <strain evidence="8 9">RF1</strain>
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        <source>
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        </source>
    </evidence>
    <sequence length="2730" mass="302600" checksum="B45940DD6BB9A949" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">RF1</strain>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="333" mass="38069" checksum="4764BA11D574CEBA" modified="2024-11-27" version="1">MEKMNSWLRSSLEALGLSNRHTDCSFVIQSPTECRVFLCHKLIFCCASEVFDRMLYGDFKESTTGEVTLKDVEPDIFEKFRDYVYGYESEKLELYGTDTLIKLCDFGNKFMVESIKEDCVNEMTNRRIHLGMGDLMLLFQCAHTLNNKSITERVVSKVEPFLREPFNQSVVLDFNTEVFKNYIETIAIKLLESDRFILIETYLKHNGLVIAAPNAGCSDASSDSGCPLVDQEITGAYVSQLLALIDFRRFTPSQFYEGPGKSNLLTLQQKYEYLYQVAEECAKTKDELRGNSFEAFRTVGLISKRHSGPYYRAAHCHCCSTRFPLNADLDLSE</sequence>
</entry>
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        <name type="scientific">Drosophila madeirensis</name>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">RF1</strain>
        </source>
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
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    </dbReference>
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        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 3 HCG-1 PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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            <end position="237"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="234"/>
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        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG00042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="265" mass="28499" checksum="0EF535C2965627AF" modified="2024-11-27" version="1">MPSTPSVETPAAQTQNRQSKTAIVVSEQKLQLQNVKRSYSVSSSVSSSASLEAAGSAASQSVATIAAVHHQRQSYSLVQKESSLRYQVKPNQHLANISLSLTMTTSETVPSLTGSSVASLGTSEATSRQCTNAKTKVTPLTSQKIHRTIPSDKINLRLILVSGKTKEFIFNPSDSAGDIAQTVFDNWPADWKREAVSKAEILRLIYQGRFLHCNVTLGALGLPLGKTTVMHLVPRDNLPEPNSQDQRQKSKGGSASCCSTTCCIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXB2</accession>
    <name>A0AAU9FXB2_DROMD</name>
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        <submittedName>
            <fullName evidence="5">Protein Tob1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_00730</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BTG family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FXB2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TOB1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.90">
        <property type="entry name" value="Anti-proliferative protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002087">
        <property type="entry name" value="Anti_prolifrtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036054">
        <property type="entry name" value="BTG-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015676">
        <property type="entry name" value="Tob1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17537">
        <property type="entry name" value="TRANSDUCER OF ERBB2 TOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17537:SF5">
        <property type="entry name" value="TRANSDUCER OF ERBB2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07742">
        <property type="entry name" value="BTG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00310">
        <property type="entry name" value="ANTIPRLFBTG1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00099">
        <property type="entry name" value="btg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160696">
        <property type="entry name" value="BTG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anti-proliferative protein" evidence="4">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="158"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="482"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="493"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFG00812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="527" mass="55325" checksum="F3F6B8A423EF9A25" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXB6</accession>
    <name>A0AAU9FXB6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Voltage-gated potassium channel subunit beta-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_00244</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19142">
        <property type="entry name" value="AKR_AKR6B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000032">
        <property type="entry name" value="Hyperkinetic, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005983">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01293">
        <property type="entry name" value="Kv_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150:SF2">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01577">
        <property type="entry name" value="KCNABCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="11">
        <location>
            <begin position="256"/>
            <end position="569"/>
        </location>
    </feature>
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        <location>
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            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
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            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
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        </location>
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            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
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            <end position="197"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG00201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="585" mass="63630" checksum="F7E9036528B847AA" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXC4</accession>
    <name>A0AAU9FXC4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_00153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FXC4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007259">
        <property type="term" value="P:cell surface receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001700">
        <property type="term" value="P:embryonic development via the syncytial blastoderm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052438">
        <property type="entry name" value="Chromatin_remod/trans_coact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031901">
        <property type="entry name" value="Unpaired"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15572">
        <property type="entry name" value="GLIOMA TUMOR SUPPRESSOR CANDIDATE REGION GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15572:SF0">
        <property type="entry name" value="GLUTAMINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15972">
        <property type="entry name" value="Unpaired"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043750987" evidence="2">
        <location>
            <begin position="23"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="361"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG00072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="462" mass="52254" checksum="B7E51A267C11F7BE" modified="2024-11-27" version="1">MCYQLPHLISLLLILALNLLLAAPAHTMAMRTASGGLTVLDANRLRTTTTTSSSSMASPSPSPSPSPSPTLSSNLSASTGSVGTGSSGYSSQPRKRHRKRNSNWIDYRNFNESTTALEWVNPCGGSYHPSVGDRFNRLRPRQSFNQLKRHAFREYRGLNSSQDSVIDIRNMTMWSLHTQNYKFLPKLKPNSTIALKRWYRNMQTYVASFAFLRRLQIRWDQRFITRESTTARELRELLLSSRRILCELETAVNQTSPRRKHRRSGPGSPNGISGGLGVQLPQISRNEMNKRLKLRSKGTGGPGTISEAITGSMPLSNEADSIDMRFVKHHYYEFVRTMWQLLRRDGKRAKKRSHRPHQKPQQQHQQQHQQQQQQQLPRKSQRERQSQAGGQQPANRSFSRLRSETSSTSSSSSNSRDFNEVSKPAMKSKSTGGISGGIGGRRGKRQSAAAGRQQRQRRVQRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXC6</accession>
    <name>A0AAU9FXC6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Probable G-protein coupled receptor B0563.6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_00149</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FXC6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14978">
        <property type="entry name" value="7tmA_FMRFamide_R-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053093">
        <property type="entry name" value="GPCR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47760">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR B0563.6-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47760:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="8">
        <location>
            <begin position="79"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG00065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="407" mass="46691" checksum="668B502B193D8FDE" modified="2024-11-27" version="1">MITRLYNTEEDPAYCSLIWGSNLSSTTDVLDGASNATPVFGTDLRDDIYRDVEDPRTQTLREYCYGIFLPIICAMGIIGNVLNLIVLTRRNMRGTAYIYMRAYSAAALLAILFAIPFGIRMQVHKDRGQWEEFGPAFYTAHLELYLGNGCLGVGVMMLLVLTLERYVSVCHPGVTRPVMGRPGLVVFVTSLVTIIVYLPSIFRGELIKCILSSPDVYVYLRRDNTIYQQTMFYKVYKIMLEMVFKLIPTMVIGGLNLQIMIVYRQTCERRRQMVLTRASSNFHKDDDPRKFAEERRLFLLLGSTSILFLVCVSPMAILHMTIASEVYPSFPFQVFRASANLMELINYSLTFYIYCLFSEDFRNTLFRTFKWPWLKGQFCHQVDLEIEAEAEAESDLPCPTEAPNLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXD8</accession>
    <name>A0AAU9FXD8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Palmitoyl-protein thioesterase 1</fullName>
            <ecNumber evidence="2">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyl-protein hydrolase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_00495</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19234"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the palmitoyl-protein thioesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="2"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FXD8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000107">
        <property type="entry name" value="Palmitoyl-protein thioesterase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002472">
        <property type="entry name" value="Palm_thioest"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247:SF8">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02089">
        <property type="entry name" value="Palm_thioest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00414">
        <property type="entry name" value="PPTHIESTRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043908358" description="Palmitoyl-protein thioesterase 1" evidence="10">
        <location>
            <begin position="20"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG00524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="298" mass="33571" checksum="A570DFD04B282798" modified="2024-11-27" version="1">MLSAAIILSLGYIIWIASADGTSGATTVLPVVLWHGMGDTCCLPFSLGSIKKLIEEHTNGTYVRSLKIGGNVVLDYESGFFVHPNEQVEYVCKHLAQDERLAKGYNAIGFSQGGQFLRAVAQRCPEPPMRTLITLGGQHQGVFGLPKCPTLTATSCEYLSRLLNEAAYEEWVQDELVQATYWHDPLHENHYRLGSTFLADINNELYLNENYIENLNKLKRFVMVKFLNDTIVQPKDSQWFQFYTSGQDQVIQPFNESKLGLDRMLREGKLQFLGIEGDHLAISKAWFIEHLVPLLLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXH4</accession>
    <name>A0AAU9FXH4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WW domain-containing adapter protein with coiled-coil homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_00474</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FXH4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000089">
        <property type="entry name" value="Blast:WW domain-containing adapter protein with coiled-coil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038867">
        <property type="entry name" value="WAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15911">
        <property type="entry name" value="WW DOMAIN-CONTAINING ADAPTER PROTEIN WITH COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15911:SF6">
        <property type="entry name" value="WW DOMAIN-CONTAINING ADAPTER PROTEIN WITH COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="5">
        <location>
            <begin position="241"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="280"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="454"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="127"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="178"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="347"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="454"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="481"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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</entry>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        </source>
    </evidence>
    <sequence length="309" mass="33748" checksum="2462383BC6BA96C8" modified="2024-11-27" version="1">MNRSNSFVSSLKWWPLQGHNSQATPAAAAAASPTSNGGYSQSAPSSPISNWPPALQQQPKAGGGVVSATFGSSVAVQKLRESKWFKPEEQRIFCAVVECGFIVEVRSSAAAEAAAAAAAAAAAAAASDIDSLDMDCPPLGLGLAPGLMIPRNGSSSISFLETQDENETPVASWYGIVLCKVAKDNKPKPDSIEIFSVKIRENGTYKLIKMSLADIWSHGWELRINNFADKEKMPHIEKDIRNQISLARKAKQSLWNNNKHFVYWCRYGSRQQDVRKRQMSECVKWGSVGMNAGMLLVLNNRQKCYSHSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0P6</accession>
    <name>A0AAU9G0P6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Ubiquitin carboxyl-terminal hydrolase 39</fullName>
            <ecNumber evidence="4">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">U4/U6.U5 tri-snRNP-associated 65 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">DMAD_01060</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26 27">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">The U4/U6-U5 tri-snRNP complex is a building block of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="4"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G0P6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02669">
        <property type="entry name" value="Peptidase_C19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000068">
        <property type="entry name" value="U4/U6.U5 tri-snRNP-associated protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000030">
        <property type="entry name" value="U4/U6.U5 tri-snRNP-associated protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033809">
        <property type="entry name" value="USP39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF16">
        <property type="entry name" value="U4_U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="16" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="25">
        <location>
            <begin position="35"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="24">
        <location>
            <begin position="158"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00079185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="BFG01267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="493" mass="57536" checksum="7FD8D0A6A1144EAC" modified="2024-11-27" version="1">MAQTKSTAKRPRLDNRQANAQEEDETPSVFNPKYRVCPYLDTINRNLLDFDFEKLCSVSLTRINVYACLVCGKYFQGRGTNTHAYTHSVGEAHHVFLNLHTLRFYCLPDNYEIIDSSLDDIKYVLNPTFTKQEISKLDLVLPKQSRTVDGILYLPGVVGLNNIKANDYCNVVLHALSHVGPLRDYFLREQNYAKIKRPPGDSMFTLVQRFGELMRKMWNPRNFKAHVSPHEMLQAVVLWSSKRFQITEQGDPIDFLSWFLNTLHRALKGNKQPNSSILYKIFLGEMQIFTRKMPPVELDDAAKEKLLATEEYQDQVEHTNFIYLTCDLPPPPLFTDEFRENIIPQVNLYQLLGKFNGSAEKEYKTYKDNFMKRFEITRLPQFIILYIKRFTKNTFFLEKNPTIVNFPIKNVDFGDILGMRQRDKDVKDTKYNLVANIVHDGDPKKGTYRAHILHKANGQWYEMQDLHVTEILPQMITLTESYIQIYERSSEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0P9</accession>
    <name>A0AAU9G0P9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Moesin/ezrin/radixin homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_01162</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
            <location evidence="4">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cell projection</location>
            <location evidence="12">Rhabdomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G0P9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098592">
        <property type="term" value="C:cytoplasmic side of apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016028">
        <property type="term" value="C:rhabdomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042051">
        <property type="term" value="P:compound eye photoreceptor development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010669">
        <property type="term" value="P:epithelial structure maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007315">
        <property type="term" value="P:pole plasm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006403">
        <property type="term" value="P:RNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13194">
        <property type="entry name" value="FERM_C_ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000003">
        <property type="entry name" value="Radixin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000002">
        <property type="entry name" value="radixin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000013">
        <property type="entry name" value="radixin isoform X1"/>
        <property type="match status" value="1"/>
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            <begin position="61"/>
            <end position="64"/>
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            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)</name>
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    <sequence length="629" mass="73992" checksum="8EAA4F62F45E5FA0" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9 10">RF1</strain>
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        <location>
            <begin position="252"/>
            <end position="320"/>
        </location>
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    <feature type="domain" description="FF" evidence="6">
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    <feature type="domain" description="FF" evidence="6">
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        <location>
            <begin position="602"/>
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    </feature>
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            <begin position="776"/>
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            <begin position="1023"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="4">
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        <source>
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        <source>
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        <source>
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                <person name="Yamamoto D."/>
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</entry>
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                <person name="Llopart A."/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="219"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="245"/>
            <end position="271"/>
        </location>
    </feature>
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        <location>
            <begin position="2014"/>
            <end position="2027"/>
        </location>
    </feature>
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        <location>
            <begin position="2087"/>
            <end position="2097"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="22">
        <location>
            <begin position="2126"/>
            <end position="2139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="2176"/>
            <end position="2195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00061650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00074738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00082498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="BFG01473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2253" mass="248717" checksum="C1D9A618343AF651" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0R5</accession>
    <name>A0AAU9G0R5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Diacylglycerol kinase</fullName>
            <shortName evidence="13">DAG kinase</shortName>
            <ecNumber evidence="13">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_01172</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="13"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9G0R5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010646">
        <property type="term" value="P:regulation of cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043052">
        <property type="term" value="P:thermotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20802">
        <property type="entry name" value="C1_DGK_typeIV_rpt1"/>
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    </dbReference>
    <dbReference type="CDD" id="cd20855">
        <property type="entry name" value="C1_DGK_typeIV_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000204">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000012">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000020">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
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    <dbReference type="InterPro" id="IPR037607">
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    </dbReference>
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        <property type="entry name" value="DGKI-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
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        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF80">
        <property type="entry name" value="EYE-SPECIFIC DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23578">
        <property type="entry name" value="DGKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DAGKc" evidence="15">
        <location>
            <begin position="404"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="967"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="1003"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="336"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="861"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="346"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="374"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG01416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1071" mass="118876" checksum="77CFDD7EBDCA77D4" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0R9</accession>
    <name>A0AAU9G0R9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Protein-tyrosine sulfotransferase</fullName>
            <ecNumber evidence="3 13">2.8.2.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_01020</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-tyrosyl-[protein] + 3'-phosphoadenylyl sulfate = O-sulfo-L-tyrosine-[protein] + adenosine 3',5'-bisphosphate + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
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        <subcellularLocation>
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            <end position="29"/>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG01209.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="347" mass="39991" checksum="4B90020A08E44943" modified="2024-11-27" version="1">MRLPYRNKKVTLWVLFGIIVITMFLFKFTELRPTCLFRVDATTNELSSQMVRVEKYVTDDNQRVYSYNREMPLIFIGGVPRSGTTLMRAMLDAHPDVRCGQETRVIPRILQLRSHWLKSEKESLRLQEAGITKEVMNSAIAQFCLEIIAKHGEPAPRLCNKDPLTLKMGSYVIELFPNAKFLFMVRDGRATVHSIISRKVTITGFDLSSYRQCMQKWNHAIEVMHEQCRDIGKDRCMMVYYEQLVLHPEEWMRKILQFLDVPWNDAVLHHEEFINKPNGVPLSKVERSSDQVIKPVNLEALSKWVGQIPGDVVRDMADIAPMLSVLGYDPYANPPDYGKGQSKTVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0S3</accession>
    <name>A0AAU9G0S3_DROMD</name>
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        <submittedName>
            <fullName evidence="2">Box A-binding factor</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">DMAD_01086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">RF1</strain>
        </source>
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            <end position="110"/>
        </location>
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        </location>
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        </location>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="807" mass="83027" checksum="A4D109DAC965D2C5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0S5</accession>
    <name>A0AAU9G0S5_DROMD</name>
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        <submittedName>
            <fullName evidence="2">Protein vreteno-like</fullName>
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        <name evidence="2" type="ORF">DMAD_01263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BFG01528.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="338"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="1">
        <location>
            <begin position="539"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG01528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="704" mass="79676" checksum="8224CC1172032433" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0V9</accession>
    <name>A0AAU9G0V9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Integrin alpha-PS2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_01353</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.930:FF:000009">
        <property type="entry name" value="integrin alpha-PS2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
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    <accession>A0AAU9G0W3</accession>
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    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_01208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G0W3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG01461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="292" mass="31808" checksum="0BD55773C48754E0" modified="2024-11-27" version="1">MNRSNSFVSSLKWWPLQGHNSQATPAAAAAASPTSNGGYSQSAPSSPISNWPPALQQQPKAGGGVVSATFGSSVAVQKLRESKWFKPEEQRIFCAVVECGFIVEVRSSAAAEAAAAAAAAAAAAAASDIDSLDMDCPPLGLGLAPGLMIPRNGSSSISFLETQDENETPVASWYGIVLCKVAKDNKPKPDSIEIFSVKIRENGTYKLIKMSLADIWSHGWELRINNFADKEKMPHIEKDIRNQISLARKAKQSLWNNNKHFVYWCRYGSRQQDVRKRQVTTSANHVLLHLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0W6</accession>
    <name>A0AAU9G0W6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein scalloped</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_00973</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G0W6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.80:FF:000003">
        <property type="entry name" value="Scalloped, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.40">
        <property type="entry name" value="TEA/ATTS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000818">
        <property type="entry name" value="TEA/ATTS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038096">
        <property type="entry name" value="TEA/ATTS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050937">
        <property type="entry name" value="TEC1_TEAD_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016361">
        <property type="entry name" value="TEF_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041086">
        <property type="entry name" value="YBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834:SF0">
        <property type="entry name" value="PROTEIN SCALLOPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01285">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17725">
        <property type="entry name" value="YBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002603">
        <property type="entry name" value="TEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00065">
        <property type="entry name" value="TEADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00426">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00554">
        <property type="entry name" value="TEA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51088">
        <property type="entry name" value="TEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TEA" evidence="9">
        <location>
            <begin position="84"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="TEA" evidence="7">
        <location>
            <begin position="84"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG01151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="434" mass="48777" checksum="486CDAAB08B8FC69" modified="2024-11-27" version="1">MKNITSSSTCSTGLLQLQNNLNSSELDIVGKSETVGPGTIPSPWTPVNAGPPGALGSADTNGSMVDSKNLDVGDMSDDEKDLSSADAEGVWSPDIEQSFQEALSIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARRKLREIQAKIKVQFWQPGLQPSTSQDIKPFAQPPYPTGKPSTAVSGEIESGIPSAQLPWEGRAIATHKFRLLEFTAFMEIQRDDMYNRHLFVQLGGKPSFSDPLLETVDIRQIFDKFPEKSGGLKDLYEKGPQNAFYLVKCWADLNTDITTGSESGDFYGVTSQYESNENIVLVCSTIVCSFGKQVVEKVESEYSRLENNRYVYRIQRSPMCEYMINFIQKLKNLPERYMMNSVLENFTILQVMRARETQETLLCIAYVFEVAAQNSGTTHHIYRLIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G109</accession>
    <name>A0AAU9G109_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Calpain-C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_01294</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G109"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00214">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000300">
        <property type="entry name" value="Calpain-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.380:FF:000018">
        <property type="entry name" value="Calpain-c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033883">
        <property type="entry name" value="C2_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022683">
        <property type="entry name" value="Calpain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF394">
        <property type="entry name" value="CALPAIN-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="3">
        <location>
            <begin position="18"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="537"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFG01578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="666" mass="76414" checksum="7D81E3DA0EED6658" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G113</accession>
    <name>A0AAU9G113_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Chromatin modification-related protein eaf-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_01728</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G113"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048998">
        <property type="entry name" value="STPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052100">
        <property type="entry name" value="SV-ATPase_mito-regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44054:SF3">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44054">
        <property type="entry name" value="SYNAPTIC VESICLE MEMBRANE PROTEIN VAT-1 HOMOLOG-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21107">
        <property type="entry name" value="STPRs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STPR" evidence="3">
        <location>
            <begin position="216"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="STPR" evidence="3">
        <location>
            <begin position="760"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="126"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="300"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="647"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="704"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="822"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="948"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="144"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="306"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="648"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="754"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="847"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="948"/>
            <end position="969"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG02140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="990" mass="108659" checksum="0FF509A2E9813390" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G126</accession>
    <name>A0AAU9G126_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Protein DDI1 homolog 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_01737</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DDI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
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    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="RP_DDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01796">
        <property type="entry name" value="Ubl_Ddi1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA damage inducible 1 homolog 2"/>
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    </dbReference>
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        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ddi1/2_HDD"/>
    </dbReference>
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        <property type="entry name" value="DDI1_N"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_aspartic_DDI1-type"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15397:SF3">
        <property type="entry name" value="DNA DAMAGE INDUCIBLE 1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15397">
        <property type="entry name" value="SODIUM-GLUCOSE COTRANSPORTER REGULATORY PROTEIN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Asp_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ddi2_HDD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="11">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
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        <location>
            <begin position="256"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="10">
        <location>
            <begin position="418"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="377"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="398"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG02155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="467" mass="51248" checksum="44F74C10462A9D28" modified="2024-11-27" version="1">MKITVTASDDRLFCLDVSHDLELENLKALCAMEIGAEVDQIVVLFNGRELTNNKHSLQQCGVNDGDFIMLERRRPNNRAGGANNPVISGLDFSSIAVPGSSATTSGGSPPSINSLQQQQQQQQMQNLTDIPMTDEFNVNFDDDPETVRQLLLSSPKTLALLREYNTRLAEALDSGDPDTFARALREHVTERKRRNDQRVRMLTADPFDEETQRLIAEEIKQKNIQDNMAAAIEYNPEIFGMVTMLYINCKVNGVPVKAFVDSGAQTTIMSKDCAERCHVNRLIDTRWNGVAKGVGTQPILGRIHMVQLQIENDHLTSSFTVLGQQPMDMLLGLDMLKRHQCLIDLQRNLLIIGTTGTTTPFLPESELPVGARLTGNPEEQAEQQAIADAMEQSRRDGASTSSSAAAGHGANTIQPMDRFTEQDVSDLMALGYPRGDVLIVLRQCGGNKQVASSLLLSRNEAAGGGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G131</accession>
    <name>A0AAU9G131_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.16</ecNumber>
            <ecNumber evidence="7">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_01084</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Dual specificity phosphatase able to dephosphorylate phosphotyrosine, phosphoserine and phosphothreonine residues, with a preference for phosphotyrosine as a substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="7"/>
    <dbReference type="EC" id="3.1.3.48" evidence="7"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G131"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033549">
        <property type="term" value="F:MAP kinase phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14515">
        <property type="entry name" value="DUSP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020405">
        <property type="entry name" value="Atypical_DUSP_subfamA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682">
        <property type="entry name" value="AGAP008228-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682:SF5">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01909">
        <property type="entry name" value="ADSPHPHTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="70"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="9">
        <location>
            <begin position="141"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="6">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR620405-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG01295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="226" mass="25708" checksum="CA32A14571A9EB1C" modified="2024-11-27" version="1">MSWRYALSTDRYSSSIGDRYSPHYYQSPSRLESSEQTTGRQLQRVLHYSMAPTRALPGLRRAECAIHDVDCDEVYPGIYIGDAAAAKNKTYLRMMGITHVLNAAEGCRYGQVDTGHSYYRDMPSIRYMGFPMIDAPTTDISRYFYVASKFIDSAISSGGKILVHCLVGMSRSATCVLAYLMICRKMSAVDAIRTVRMRRDIRPNDGFLQQLADLDMELKRKNLYPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G133</accession>
    <name>A0AAU9G133_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Venom allergen-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_01153</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISP family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G133"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000007">
        <property type="entry name" value="Venom allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034763">
        <property type="entry name" value="P14a_insect"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038921">
        <property type="entry name" value="P14a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043448610" description="Venom allergen-1" evidence="6">
        <location>
            <begin position="29"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="7">
        <location>
            <begin position="69"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00068306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFG01392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="259" mass="28613" checksum="701BF5B1511A297B" modified="2024-11-27" version="1">MPVMRHRQSFARLFPAFLLAVLYPLAAGQNYCDPELCPSGRHVACQNSGRFVSGCSGEFVPIDPHIPLILQLHNERRNQIAGGGLSGFPSAVHMATMSWDPTLAQLAAYNALQCRMAHDECRNTNTYRYAGQNLSILFTRSGDVADFLRQRIAAWFDENRYATSGDMDNYQMRGGPAIGHFTTMVNERNSRVGCAIARFTDANNVQATLLACDYAVTNVLNNPVYRAGAPASECTSGRNPTYPNLCAVNENYNFNQWSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G134</accession>
    <name>A0AAU9G134_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Troponin T skeletal muscle</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_01021</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin T family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G134"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000003">
        <property type="entry name" value="Troponin T isoform 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027707">
        <property type="entry name" value="TNNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521">
        <property type="entry name" value="TROPONIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521:SF1">
        <property type="entry name" value="TROPONIN T, SKELETAL MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="282"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="36"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="83"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="330"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFG01211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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                <person name="Yamamoto D."/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G140</accession>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1307"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1764"/>
            <end position="1782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1869"/>
            <end position="1879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1880"/>
            <end position="1898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG01623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2125" mass="234110" checksum="334EFA0E4027FE10" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G145</accession>
    <name>A0AAU9G145_DROMD</name>
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        <recommendedName>
            <fullName evidence="5">Moesin/ezrin/radixin homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_01162</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
            <location evidence="4">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cell projection</location>
            <location evidence="12">Rhabdomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G145"/>
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        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098592">
        <property type="term" value="C:cytoplasmic side of apical plasma membrane"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
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    </dbReference>
    <dbReference type="GO" id="GO:0005902">
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    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016028">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0007315">
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="FERM_C_ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FERM_F1_ERM"/>
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    </dbReference>
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        <property type="entry name" value="Radixin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000002">
        <property type="entry name" value="radixin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000013">
        <property type="entry name" value="radixin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.450:FF:000001">
        <property type="entry name" value="radixin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
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    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011174">
        <property type="entry name" value="ERM"/>
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    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
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        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
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        <property type="entry name" value="Moesin_tail_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23281">
        <property type="entry name" value="MERLIN/MOESIN/EZRIN/RADIXIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20492">
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    </dbReference>
    <dbReference type="Pfam" id="PF09380">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BAND41"/>
    </dbReference>
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        <property type="entry name" value="ERMFAMILY"/>
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        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48678">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="459"/>
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        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="303"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="472"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
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            <end position="554"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="61"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)</name>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)</name>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR002305-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG01402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="575" mass="67900" checksum="4410AFA70CC4ADF9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G172</accession>
    <name>A0AAU9G172_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Integral membrane protein DGCR2/IDD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_01758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G172"/>
    <dbReference type="Proteomes" id="UP001500889">
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        <source>
            <dbReference type="EMBL" id="BFG02185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="16594" checksum="86B855F56FEFBE1E" modified="2024-11-27" version="1">MTSLWLLLPSNATSWLLLALLCLLGCLCPFTDALEEDCVDFQGNKVNHGMLYVPGPGVCSLCVCYHSEPLWCKAIYCDPPYFCKNFRVGERCCEFECLDPPGEDKLYQERMRKRAEILAGNSTATNVRLAPLAMSSIVLGFLGNSFLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G173</accession>
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        <submittedName>
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        </submittedName>
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        <name type="scientific">Drosophila madeirensis</name>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="entry name" value="Sarcospan"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="255"/>
            <end position="275"/>
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            <begin position="287"/>
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    </feature>
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            <begin position="325"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="411"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="147"/>
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            <begin position="1"/>
            <end position="13"/>
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    </feature>
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            <begin position="36"/>
            <end position="48"/>
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            <begin position="50"/>
            <end position="66"/>
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    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG01546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="448" mass="48905" checksum="6407779124BA5906" modified="2024-11-27" version="1">MQSQQVRPISTYDNLGAAAHVAPSNKPHQQQQQLHRAGESPKQPDRATTRGVSDQLLSNGMSQPQTELELEPEEELNGSGTSHSIAAVKAALNDAKSKFFGINNYDEYESAVQPTQMPMPVVKAHSQQPTIKPEPKYQNVPQRPKPQPDELPATIIEPVAMPVSVSSPVDRSLRYATTYEQIPLSDLDAPQPSQAVYMSTTVPQNIKGMKIAGRHTPTRNSLRHSRMIVVNHTNHDSLQAAYSKHIRNLNISRQLLIMQLAVGLLIVGLAIWILLLEPNASILINPYLSGLSLLLASIAGLILLRRDHRTQGHRAPNSCYKVLLAESYVFSALALVFCCLALVCAAIEFAELASAVDGACGPATSSLLSYHNCTCLTSGDDVAADGSSSPNDSSSSSSFEECSAFRVEWKYLLAFSMALNALGIVATFLYITLFICCHRNRKHFYTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G174</accession>
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        <recommendedName>
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    <organism evidence="14 15">
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="14 15">RF1</strain>
        </source>
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    <comment type="function">
        <text evidence="10">Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000414">
        <property type="entry name" value="Eukaryotic translation initiation factor 4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034229">
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    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF11">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4H"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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            <end position="108"/>
        </location>
    </feature>
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            <end position="301"/>
        </location>
    </feature>
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            <begin position="114"/>
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        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG02153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="343" mass="36184" checksum="95E2812A3FBC4889" modified="2024-11-27" version="1">MAGRGGYEHARGGFGGERQMKVLPTEPPFIAFVGNLPQGLVQGDVMKIFQDFEVKNVRLVKDRETDMFKGFCYVEFETLENLERALEFDGRIKLDDLSAPLRIDIADRRKNDRPGGGVGGGNGGMTRDGGRDGFQKRGPPRQGGTSQSYSRGGPGTGGNGGGGREGGGGSGNRADSRDRPANRGRYGNFNNDDRFDRNQDRDRGQREGSYGNQSRDTDRYNNFSRHRDRERTHYNPNQQSERPSGGLGGGGGGLGGSGGLGGSGNLGGGGGGGGGGGGGGGGASIGAIDDTERPRLQLKPRTIAAPINAVAETKQSASIFGNAKPREEKLKELEQNVSHNGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G177</accession>
    <name>A0AAU9G177_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Integumentary mucin C.1</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ORF">DMAD_01183</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
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    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043392555" evidence="3">
        <location>
            <begin position="22"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="110"/>
        </location>
    </feature>
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        <location>
            <begin position="163"/>
            <end position="237"/>
        </location>
    </feature>
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        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="318" mass="33458" checksum="8DBDEEC68B5744A5" modified="2024-11-27" version="1">MNVLPLLWLLCSSSLVLLAAGEQNATVASVGAANPQTAQSNSSTAAPLVVNVMNATVSAQAAVPGQVHIIEKTQVKSTNSTELAKEHEKDMNKTSTIPPDNTAKTRKRTDPVQLKPTIATAAPTSAAPAAPNGTITKAAAATATAKPATGNIVAEGISISDVKKVNSSSTTSGNSSSTSTTPSTTTTTTKTTTTTTTTTTPRPTKPAVVYSMDTHSEWEKEQEDKQKSPAAPSLAASSEPLPVLASTQPEPAAPMVQELTGNLADRRGNDYIVPIVTVLLTVPLAIGVVTIMYRRFRELWSTRHYRRMDFLVDGMYND</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G187</accession>
    <name>A0AAU9G187_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">furin</fullName>
            <ecNumber evidence="16">3.4.21.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_01284</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Release of mature proteins from their proproteins by cleavage of -Arg-Xaa-Yaa-Arg-|-Zaa- bonds, where Xaa can be any amino acid and Yaa is Arg or Lys. Releases albumin, complement component C3 and von Willebrand factor from their respective precursors.</text>
            <dbReference type="EC" id="3.4.21.75"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.75" evidence="16"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000001">
        <property type="entry name" value="Furin 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000020">
        <property type="entry name" value="Furin-like protease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000055">
        <property type="entry name" value="Furin-like protease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000096">
        <property type="entry name" value="Furin-like protease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000072">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.850:FF:000001">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.850">
        <property type="entry name" value="Peptidase S8, pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032778">
        <property type="entry name" value="GF_recep_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032815">
        <property type="entry name" value="S8_pro-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF23">
        <property type="entry name" value="FURIN-LIKE PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14843">
        <property type="entry name" value="GF_recep_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16470">
        <property type="entry name" value="S8_pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 19 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 19 20" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 19 20" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1456"/>
            <end position="1481"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="23">
        <location>
            <begin position="675"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="273"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="385"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="280"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="18 19">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="18 19">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="18 19">
        <location>
            <position position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00053600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="BFG01561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1641" mass="179279" checksum="723D4BBD54A6D8AE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G9A5</accession>
    <name>A0AAU9G9A5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">UPAR/Ly6 domain-containing protein qvr</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Protein quiver</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Protein sleepless</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_03587</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Bifunctional regulator of neuronal activity in the mushroom body, and possibly other regions of the brain, that acts as a signaling molecule required for homeostatic regulation of sleep under normal conditions and after sleep deprivation. Reduces neuronal excitability by enhancing Sh/shaker K(+) channel activity; possibly by stabilizing Sh/shaker to increase protein levels, accelerating its activation kinetics, slowing C-type inactivation and enhancing recovery from inactivation. Specifically affects the A-type K(+) current. Antagonizes nicotinic acetylcholine receptors (nAChRs) to reduce synaptic transmission, possibly by preventing their localization to the cell surface. Required for regulation of neuromuscular excitability and plasticity at neuromuscular junctions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts (via loop 2 of the three-fingered Ly-6 domain) with Sh/shaker; this interaction may stabilize both components of the complex and may be required for targeting or retention of Sh/shaker to neural cell projections. Interacts (via loop 2 of the three-fingered Ly-6 domain) with nAChRalpha3 and potentially other nicotinic acetylcholine receptors; this interaction is required for antagonism of nicotinic acetylcholine receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
            <orientation evidence="1">Extracellular side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane raft</location>
            <topology evidence="14">Lipid-anchor</topology>
            <topology evidence="14">GPI-anchor</topology>
            <orientation evidence="14">Extracellular side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the quiver family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G9A5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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</entry>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <source>
            <dbReference type="EMBL" id="BFG04739.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="217" mass="23496" checksum="730CB1571ED3709B" modified="2024-11-27" version="1">MIERRSQLDKIIITAMDRFVWFVLLVVMSHLVTEFTRVSGYARGQRHELVLLNDTMLQPMPVQNMLLYPSHEYGQYQQRPSSMNSLQQNAALLFSSLAAGAAAGTGAGTGASAEPSSNAALLAVGDSQPAHSTEPSYPLFSLRPLIDSIFEIPISTLRAVNNMVGRLTGGYRQATTEASQKSVAAGTLLPSGNPSTTANTGSKYSTSQRRELREEMS</sequence>
</entry>
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    <accession>A0AAU9G9B8</accession>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <authorList>
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                <person name="Yamamoto D."/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF10">
        <property type="entry name" value="SLOWPOKE 2, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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        </location>
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        </location>
    </feature>
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            <end position="300"/>
        </location>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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        </location>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="1620" mass="177825" checksum="78318CC51D7D6FA9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G9D2</accession>
    <name>A0AAU9G9D2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_03905</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in vesicular protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the VPS35 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G9D2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030906">
        <property type="term" value="C:retromer, cargo-selective complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.660:FF:000005">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.660">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35, helical subcomplex Vps35-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005378">
        <property type="entry name" value="Vps35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042491">
        <property type="entry name" value="Vps35_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03635">
        <property type="entry name" value="Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009375">
        <property type="entry name" value="Retromer_Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG05087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="803" mass="91097" checksum="774BD18E1A575C37" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G9D4</accession>
    <name>A0AAU9G9D4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ran-binding proteins 9/10 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_03113</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RANBP9/10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G9D4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="CDD" id="cd12909">
        <property type="entry name" value="SPRY_RanBP9_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000011">
        <property type="entry name" value="RAN binding protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035782">
        <property type="entry name" value="SPRY_RanBP9/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="3">
        <location>
            <begin position="29"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="CTLH" evidence="4">
        <location>
            <begin position="284"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="350"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="448"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFG04061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="608" mass="67420" checksum="51372B4DFB9D266F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G9D8</accession>
    <name>A0AAU9G9D8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WD repeat-containing protein 48 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_03492</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Regulatory component of the Usp12-46 deubiquitylase complex. activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. The complex deubiquitylates the wg/wingless-signaling receptor arr/arrow, which stabilizes the receptor and increases its concentration at the cell surface; this enhances the sensitivity of cells to wg/wingless-signal stimulation. This increases the amplitude and spatial range of the signaling response to the wg/wingless morphogen gradient, facilitating the precise concentration-dependent regulation of its target genes. Together with Wdr20 and Usp12-46 required for wg/wingless-mediated signaling in the wing imaginal disc and for wg/wingless-dependent regulation of intestinal stem cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Catalytic component of the Usp12-46 deubiquitylase complex consisting of Usp12-46, Wdr20 and Uaf1; regulatory subunit that, together wtih Wdr20, stabilizes Usp12-46. The Usp12-46 deubiquitylase complex associates with arr/arrow; the interaction leads to deubiquitination and stabilization of arr/arrow.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat WDR48 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G9D8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17041">
        <property type="entry name" value="Ubl_WDR48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000543">
        <property type="entry name" value="WD repeat-containing protein 48 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000984">
        <property type="entry name" value="WD repeat-containing protein 48 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051246">
        <property type="entry name" value="WDR48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021772">
        <property type="entry name" value="WDR48/Bun107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19862">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19862:SF14">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11816">
        <property type="entry name" value="DUF3337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="12"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="57"/>
            <end position="98"/>
        </location>
    </feature>
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            <end position="140"/>
        </location>
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        <location>
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            <end position="182"/>
        </location>
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    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="192"/>
            <end position="233"/>
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            <end position="606"/>
        </location>
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        <location>
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            <end position="597"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00006917"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00021538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG04548.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="668" mass="75103" checksum="3DAD1AC3680EF329" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G9D9</accession>
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            <fullName evidence="13">Tafazzin family protein</fullName>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="16 17">RF1</strain>
        </source>
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        <reaction evidence="11">
            <text>1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3-phospho]-glycerol + a 1,2-diacyl-sn-glycero-3-phosphocholine = a cardiolipin + a 1-acyl-sn-glycero-3-phosphocholine</text>
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            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="ChEBI" id="CHEBI:62237"/>
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        </reaction>
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            <dbReference type="Rhea" id="RHEA:33733"/>
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    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-hexadecanoyl-sn-glycero-3-phosphocholine = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:43816"/>
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            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73001"/>
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            <dbReference type="Rhea" id="RHEA:43818"/>
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            <orientation evidence="1">Intermembrane side</orientation>
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        <subcellularLocation>
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        </subcellularLocation>
    </comment>
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        <text evidence="2 13">Belongs to the taffazin family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
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        <property type="entry name" value="Tafazzin"/>
    </dbReference>
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        <property type="entry name" value="TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TAZ PROTEIN TAFAZZIN"/>
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    </dbReference>
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        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="111"/>
            <end position="134"/>
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            <begin position="158"/>
            <end position="282"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG04558.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="354" mass="41065" checksum="CBDAA41A51AE2025" modified="2024-11-27" version="1">MRSSVRRAKRKQKHCSIFNSQYVQAPETRPVPDERSTAGLQKQEQTEQRPATTRHPVSQQALSSQPIQTQPPPPQPPTMSDKEPRMNMPTDVVIPYNIDWVFERMRCPSRVWWLASHFVIAAVGIFSKILLMFVNKTRVYNKELLVDLISKRPKGVGLLTVSNHYSCLDDPGLWGMLPLRQACNTSCVRWSMAAHDICFTNKYHSMFFMFGKCIPVVRGYGVYQEAINLCIEKCATGQWVHVFPEGKVNMEKEELRLKWGVGRIIYDSPKMPIILPLWHEGMDDVLPNVEPYVPQWRKKVTINIGQPLDLNDFVQDLKNKQVPEQTARKLITDKIQEVFRILRTETEQLHRERQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAQ0</accession>
    <name>A0AAU9GAQ0_DROMD</name>
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        <submittedName>
            <fullName evidence="5">Pupal cuticle protein 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_03487</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BFG04541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAQ0"/>
    <dbReference type="Proteomes" id="UP001500889">
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        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CUTICLE"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043459860" evidence="4">
        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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            <begin position="604"/>
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        </location>
    </feature>
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        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
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        <location>
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            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="604"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFG04541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="692" mass="56101" checksum="0178AF4ECB9E5D34" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAQ2</accession>
    <name>A0AAU9GAQ2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Titin homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_04260</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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        <property type="entry name" value="PROTEIN RIC-3"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21723">
        <property type="entry name" value="RESISTANCE TO INHIBITORS OF CHOLINESTERASE PROTEIN 3 RIC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15361">
        <property type="entry name" value="RIC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Resistance to inhibitors of cholinesterase protein 3 N-terminal" evidence="9">
        <location>
            <begin position="38"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="274"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="274"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF15361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG04821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="463" mass="50278" checksum="F6C92025AE04EEA0" modified="2024-11-27" version="1">MPSAAVAPSQPATAKKRPSAVKEEGMTPKKTALIIVTVIGCIAILWPKVFHPMMFGSAPLPKQQNPKEQRSGAPGGCCDVVLDREQFMNASKPDAAVPFGPHLYRKHVNLYTGEISLRQERPAHLHPESIYQAMRERGRAIPATPTVPIVERKSSPSNPPPRIVDGRPGPIPGMRPPMGAGALQQPQQRGSSMGFLMPLYTIGIVVFFGYTIMKIAFKKQVPNAPYGPAPTDPGFRQQVFGQQAHSQVEDLGGTKLVVTAIQGLIDAADEQLNVRDKQRSDTDTETDLNKQNDSEKTKEQLEKENISNGHVNTISDQGAAGKQQQRKRRDLSAERELTVLGMEVTASCEGGGKWTGRPPTPVFRAPSEHSKLEGNLPEPQSIYLEGALAHDSQILVTDAQTKREEVYDAELNGSAEEPAVILSSKMTLSLINLDANQQNGNVGKPGIESPLADDIEIIGHDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAQ6</accession>
    <name>A0AAU9GAQ6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Dystonin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_03839</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030056">
        <property type="term" value="C:hemidesmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21189">
        <property type="entry name" value="CH_PLEC-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
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        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
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        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
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        <property type="entry name" value="microtubule-actin cross-linking factor 1 isoform X13"/>
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        <property type="entry name" value="microtubule-actin cross-linking factor 1 isoform X21"/>
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        <property type="entry name" value="Short stop, isoform AA"/>
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        <property type="entry name" value="Short stop, isoform H"/>
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    </dbReference>
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        <property type="entry name" value="Short stop, isoform N"/>
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        <property type="match status" value="29"/>
    </dbReference>
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        <property type="entry name" value="Calponin-like domain"/>
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        <property type="entry name" value="Gas2-like domain"/>
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        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="11"/>
    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
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        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Desmoplakin_SH3"/>
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    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
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        <property type="entry name" value="GAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036534">
        <property type="entry name" value="GAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049538">
        <property type="entry name" value="PCN-like_spectrin-like_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043197">
        <property type="entry name" value="Plakin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035915">
        <property type="entry name" value="Plakin_repeat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001101">
        <property type="entry name" value="Plectin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169">
        <property type="entry name" value="ENVOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169:SF23">
        <property type="entry name" value="SHORT STOP, ISOFORM H"/>
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        <property type="entry name" value="CH"/>
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        <property type="match status" value="16"/>
    </dbReference>
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        <property type="entry name" value="Spectrin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21020">
        <property type="entry name" value="Spectrin_4"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="29"/>
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        <property type="match status" value="32"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51460">
        <property type="entry name" value="GAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="12">
        <location>
            <begin position="230"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="881"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="8346"/>
            <end position="8381"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="8382"/>
            <end position="8417"/>
        </location>
    </feature>
    <feature type="domain" description="GAR" evidence="14">
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <name type="scientific">Drosophila madeirensis</name>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12 13">RF1</strain>
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        <text evidence="6">Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed.</text>
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        <text evidence="9">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
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        <text evidence="2 10">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1488"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1910"/>
            <end position="1919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1969"/>
            <end position="1980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="2006"/>
            <end position="2020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="2030"/>
            <end position="2045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="2317"/>
            <end position="2330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="2332"/>
            <end position="2347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="2366"/>
            <end position="2378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="2379"/>
            <end position="2389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="2393"/>
            <end position="2405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="2420"/>
            <end position="2442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="2445"/>
            <end position="2455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="2563"/>
            <end position="2582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG04756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2599" mass="284575" checksum="47CF3004E48FE0A2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAS1</accession>
    <name>A0AAU9GAS1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Trehalase</fullName>
            <ecNumber evidence="3 9">3.2.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Alpha-trehalose glucohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_03702</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 37 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="3 9"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAS1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000034">
        <property type="entry name" value="Trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001661">
        <property type="entry name" value="Glyco_hydro_37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018232">
        <property type="entry name" value="Glyco_hydro_37_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403:SF1">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01204">
        <property type="entry name" value="Trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00744">
        <property type="entry name" value="GLHYDRLASE37"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00927">
        <property type="entry name" value="TREHALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00928">
        <property type="entry name" value="TREHALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043403826" description="Trehalase" evidence="11">
        <location>
            <begin position="21"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="592"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG04836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="613" mass="69801" checksum="421FEC36F1588C38" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAS2</accession>
    <name>A0AAU9GAS2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_04035</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAS2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043089003" description="Carboxylic ester hydrolase" evidence="6">
        <location>
            <begin position="31"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="7">
        <location>
            <begin position="41"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFG05264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="593" mass="66338" checksum="C6120BC3DB0040AC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAS3</accession>
    <name>A0AAU9GAS3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_03605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAS3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006631">
        <property type="entry name" value="DM4_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21398">
        <property type="entry name" value="AGAP007094-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21398:SF22">
        <property type="entry name" value="IP12060P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07841">
        <property type="entry name" value="DM4_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00718">
        <property type="entry name" value="DM4_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG04707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="234" mass="26339" checksum="1F27BEABE8DA71B9" modified="2024-11-27" version="1">MNWQPANSALLYTTNSEFGIFMAISVPIGLPHRNVFLSYNYEFNYYQPEHVYKYPPILMGDYQDSYLTYPTSGREDSGRHCQNCTERRDEQMDTSSSSSSSSSGSSNSNSTAESRGKRSLSLMSRSIFYSMLRDKLQRSGYPADACLLRMICETNASQLGEVNGFLGSLIHIIFTPSSSKDEHLAEEFYQAEWDGRQRGECSGYTANCEANILDLISVPVEQALSDIVSGRRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAS5</accession>
    <name>A0AAU9GAS5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_04386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAS5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG05710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="143" mass="16544" checksum="E00C11B640FFD62A" modified="2024-11-27" version="1">MLPYEWQGVPVRTAIAVLSLIVSIVLLLLIPTLVEPRDSWEISVWYHVCLLQAIGGVLLIVGSLKSNHWMYLPWCVAAIVFIYTLLYKTVSYYCYLQSKPVYLVIPLFYTIAGFWLYFLCDVFQDFLDLHRKSSPQILSHDPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAS7</accession>
    <name>A0AAU9GAS7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutaminase</fullName>
            <ecNumber evidence="3">3.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">L-glutamine amidohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_03510</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="3"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAS7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:L-glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.210:FF:000002">
        <property type="entry name" value="Blast:Glutaminase kidney isoform, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000069">
        <property type="entry name" value="Glutaminase, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.710.10:FF:000008">
        <property type="entry name" value="Glutaminase, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00313">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015868">
        <property type="entry name" value="Glutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041541">
        <property type="entry name" value="Glutaminase_EF-hand"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03814">
        <property type="entry name" value="Gln_ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544">
        <property type="entry name" value="GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544:SF29">
        <property type="entry name" value="GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17959">
        <property type="entry name" value="EF-hand_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04960">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Glutaminase EF-hand" evidence="11">
        <location>
            <begin position="128"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="585"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="649"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="668"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00077251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG04569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="714" mass="79471" checksum="0A68881B4D0D979C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAS9</accession>
    <name>A0AAU9GAS9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sialin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_04281</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAS9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17318">
        <property type="entry name" value="MFS_SLC17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000157">
        <property type="entry name" value="Inorganic phosphate cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000003">
        <property type="entry name" value="Solute carrier family 17 member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF336">
        <property type="entry name" value="LP19554P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0769">Symport</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="349"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="15"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG05582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="461" mass="51052" checksum="F637F14A38BDE703" modified="2024-11-27" version="1">MPCFYVPKRINVAIMLFMACLLSYMMRVNLSINIIAMVEDTSNNENGTVEVLPDYGPRYNWTQSDQALLLGAYFYGYMITSLPAGTLAEMLGARNVAGYSCLVAGILTALTPAAAAWDKYAVFAVRFLIGFLNGVVYPCCHSLVSKWSPPDEKGKFVASLMGGTFGTVITWPISGVIIENLGWDWAYYIVGIFVLIVVAVWFYLVADTPAQHSTISIKEREYIENSLGNTLSNKKKWPPYKELVLSMPFWSLMLLHYGSMWGLFFLITATPKFLSEVLGFNLSSAGVLSSLPHVARLICAFGFGGVADWIRRRGWWTVTKMRKAFCLPSHILPGVMLIILAYFGRDPYVCVAIMTISLGFNGAATASNLANSQDLAPNYAGTLYGIINCVGTTPGIFSPLIVAAFTEKENTIDQWHWIFIIGAAAYILPALFFWAFGSGKIQKWNEVETTESREDIVNTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAW2</accession>
    <name>A0AAU9GAW2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_03630</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAW2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043437475" evidence="2">
        <location>
            <begin position="24"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG04737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="246" mass="24765" checksum="F929A77EF59686AF" modified="2024-11-27" version="1">MRKMFAFIGVLCLLQSAIPLVPAQLITLRDGKVGVNFAGYHADAGLGGLLTGNSAHGGLSASAGTPWGSRAAAGLGGNLDGSTNGFGYAAAQANPSVGASALLGGSAGEHGYIGAEAHSPGKTAITSKQHTVQHQAPAAASPTSGPAAPPALPTVSNHVQRVKPPKKYLLIRQDVPAKSDVKTVQTHKYTKTVQRSPQAVGNRFLAPQSSSPVGLALDIPIGILRSLQQSLGNLANGAPQAAPVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAW4</accession>
    <name>A0AAU9GAW4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">F-box/LRR-repeat protein 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_03578</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="F-box_FBXL2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000060">
        <property type="entry name" value="F-box/LRR-repeat protein 20 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000461">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF165">
        <property type="entry name" value="F-BOX_LRR-REPEAT PROTEIN FBXL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="65"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG04670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="479" mass="53260" checksum="E3EAC9D1E65E9C59" modified="2024-11-27" version="1">MSDCSSSSLSVSPDAACLADNDDEDQQQRQPLQRSEAGSTDSYLAKDSDVAQQPQTFLGATELDDELIKQLPKEVLLRVFSYLDVVSLCRCAQVCKYWNVLALDGSSWQKINLFDFQRDIEGPVIENISQRCRGFLKSLSLRGCQSLGDQSVRTLANHCHNIEHLDLSECKKITDISTQSISRYCTKLTAINLDSCSNITDNSLKYLSDGCPNLMEINVSWCHLISENGVEALARGCVKLRKFSSKGCKQINDNAIMCLAKYCPDIMVLNVHSCETISDSSIRQLAAKCPKLQKLCVSKCADLTDLSLMALSQHNHLLNTLEVSGCRNFTDIGFQALGRNCKYLERMDLEECNQITDLTLAHLATGCPGLEKLTLSHCELITDDGIRHLTTGSCAAEILSVLELDNCPLITDRTLEHLVSCHNLQRIELFDCQLITRTAIRKLKNHLPNIKVHAYFAPGTPPAVTSGQRPRYCRCCEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAX1</accession>
    <name>A0AAU9GAX1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_03540</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAX1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17301">
        <property type="entry name" value="PIPKc_PIP5KI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000001">
        <property type="entry name" value="phosphatidylinositol 4-phosphate 5-kinase type-1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF101">
        <property type="entry name" value="LP03320P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="5">
        <location>
            <begin position="79"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="342"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="395"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="514"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="731"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="352"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="560"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="587"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="634"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="669"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="751"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFG04614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="804" mass="88815" checksum="BDFF7864842BABBB" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAX9</accession>
    <name>A0AAU9GAX9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">UPAR/Ly6 domain-containing protein qvr</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Protein quiver</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Protein sleepless</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_03587</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Bifunctional regulator of neuronal activity in the mushroom body, and possibly other regions of the brain, that acts as a signaling molecule required for homeostatic regulation of sleep under normal conditions and after sleep deprivation. Reduces neuronal excitability by enhancing Sh/shaker K(+) channel activity; possibly by stabilizing Sh/shaker to increase protein levels, accelerating its activation kinetics, slowing C-type inactivation and enhancing recovery from inactivation. Specifically affects the A-type K(+) current. Antagonizes nicotinic acetylcholine receptors (nAChRs) to reduce synaptic transmission, possibly by preventing their localization to the cell surface. Required for regulation of neuromuscular excitability and plasticity at neuromuscular junctions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts (via loop 2 of the three-fingered Ly-6 domain) with Sh/shaker; this interaction may stabilize both components of the complex and may be required for targeting or retention of Sh/shaker to neural cell projections. Interacts (via loop 2 of the three-fingered Ly-6 domain) with nAChRalpha3 and potentially other nicotinic acetylcholine receptors; this interaction is required for antagonism of nicotinic acetylcholine receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
            <orientation evidence="1">Extracellular side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane raft</location>
            <topology evidence="14">Lipid-anchor</topology>
            <topology evidence="14">GPI-anchor</topology>
            <orientation evidence="14">Extracellular side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the quiver family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAX9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032222">
        <property type="term" value="P:regulation of synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030431">
        <property type="term" value="P:sleep"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23595">
        <property type="entry name" value="TFP_LU_ECD_Qvr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031424">
        <property type="entry name" value="QVR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050975">
        <property type="entry name" value="Sleep_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33562">
        <property type="entry name" value="ATILLA, ISOFORM B-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33562:SF31">
        <property type="entry name" value="PROTEIN QUIVER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17064">
        <property type="entry name" value="QVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043650464" description="UPAR/Ly6 domain-containing protein qvr" evidence="20">
        <location>
            <begin position="29"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="BFG04685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="157" mass="17995" checksum="ACC270EA9EDF3979" modified="2024-11-27" version="1">MWTEKGNAVGSWLLAFTVFLALAKFATTDNCFPTRSIYCYECDSWTDARCKDPFNYTALPRDQPPLMTCNGCCVKMVRHQRSPYEVVRRMCTSQLQINLFMVDHVCMMESSGNGHMCFCEEDMCNSSKDLYTHGHQLHLIITIAVAVSWLMGQLLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GB21</accession>
    <name>A0AAU9GB21_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Trehalase</fullName>
            <ecNumber evidence="3 9">3.2.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Alpha-trehalose glucohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_03702</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 37 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="3 9"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GB21"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000034">
        <property type="entry name" value="Trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001661">
        <property type="entry name" value="Glyco_hydro_37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018232">
        <property type="entry name" value="Glyco_hydro_37_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403:SF1">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01204">
        <property type="entry name" value="Trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00744">
        <property type="entry name" value="GLHYDRLASE37"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00927">
        <property type="entry name" value="TREHALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00928">
        <property type="entry name" value="TREHALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043672784" description="Trehalase" evidence="10">
        <location>
            <begin position="21"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG04837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="591" mass="67611" checksum="AA43EB8F8FB9718F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GB28</accession>
    <name>A0AAU9GB28_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein espinas</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_04121</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prickle / espinas / testin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GB28"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09415">
        <property type="entry name" value="LIM1_Prickle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09418">
        <property type="entry name" value="LIM2_Prickle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09420">
        <property type="entry name" value="LIM3_Prickle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09827">
        <property type="entry name" value="PET_Prickle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000022">
        <property type="entry name" value="prickle-like protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000035">
        <property type="entry name" value="prickle-like protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000005">
        <property type="entry name" value="Testin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033725">
        <property type="entry name" value="LIM1_prickle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033726">
        <property type="entry name" value="LIM2_prickle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033727">
        <property type="entry name" value="LIM3_prickle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010442">
        <property type="entry name" value="PET_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033723">
        <property type="entry name" value="PET_prickle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047120">
        <property type="entry name" value="Pk/Esn/Tes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24211">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24211:SF20">
        <property type="entry name" value="PROTEIN ESPINAS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06297">
        <property type="entry name" value="PET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51303">
        <property type="entry name" value="PET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PET" evidence="10">
        <location>
            <begin position="145"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="252"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="317"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="67"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="437"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="630"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="67"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="469"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="642"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="656"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="672"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00068621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG05369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="799" mass="87868" checksum="66563296BC0F847B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GB29</accession>
    <name>A0AAU9GB29_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cell wall protein DAN4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_03789</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GB29"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23307">
        <property type="entry name" value="beta-trefoil_FGF8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043706534" evidence="3">
        <location>
            <begin position="21"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="407"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="518"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="279"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="336"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="523"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="568"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="593"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="610"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG04941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="783" mass="85659" checksum="C66844D72A48CB75" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GB31</accession>
    <name>A0AAU9GB31_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Nucleoporin seh1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_03962</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat SEC13 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GB31"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000063">
        <property type="entry name" value="SEH1 like nucleoporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037363">
        <property type="entry name" value="Sec13/Seh1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SEC13 / SEH1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024:SF3">
        <property type="entry name" value="NUCLEOPORIN SEH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="10" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="5 13" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="8"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="268"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFG05163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="354" mass="39316" checksum="0A8E97F5E653161A" modified="2024-11-27" version="1">MFDVEPIIADHKDVIHDVVFDYYGRRMATCSSDQTVKIWDEDAQGKWSVTSSWKAHSGSIWRVSWAHPEFGQVVATCSFDRTASVWEEVIGEKVSTTNTPTRRWVRRTTLVDSRTSVTDVEFAPKYLGLLLATASADGIIRIYEAPDIMNLSQWPVQHEIANKLPLSCLSWNTSTYMVTQLLAAGSDDSATPTGKVFLFAYSDNARKCVKIDTVNDITDPVTDVAFAPNAGRTFHMLAVASKDLYIVNLRGVTDSTGTTKLDIQTVKFSDHNCPVWRVCWNMLATMLISTGDDGCVRLWRMNYNKQWRCAAVLKAEGGGPTYEPAPPTPTLAATASATAKFYKKGTIGNQVPWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GB33</accession>
    <name>A0AAU9GB33_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PDZ and LIM domain protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_04071</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GB33"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF38">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="37"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="163"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFG05314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="251" mass="28203" checksum="0F5F81602076B3BB" modified="2024-11-27" version="1">MPEQLGLQWSTPESKKNAPIIKMSCGIGDTDGFPAFDVDSEAYASKDDTKWGFLVTGGAEFHMPLTVFQVTPDGLADKAGIRLGDIILQINEEDATQLTLNQAHEKINASPKKIHFLLRNMEEDDPMGQFEAGEEKSIVMRVPKPMPPPSDFHPTPLLGSRCWHPIMWQDPPEPPKPKQKKTKTQLDDEGNEVVIELSEDESSDEEEQELPHHRIIRNIRRFFAEVGDNRELRSSTIENMLLALPSAPKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GB37</accession>
    <name>A0AAU9GB37_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_04518</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GB37"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044713102" evidence="2">
        <location>
            <begin position="20"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG05888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="440" mass="49620" checksum="46CD6C002AF65D4A" modified="2024-11-27" version="1">MEVLCKILAVCTLLVGAQAFVAVEPASSSSSELSWKSLFFDALVGNSDELQAAGSLGSLEECTAIYTVENVEILLSAGYKIRRCVKVAEKDISRDLKDAKKEFERELRSCRSRDCYVDSIMNLFSFVRLSRHDYRRSRHCILKEVEQALVQLDRASIVLANCLADQQPPQNSTLPPPIWNTTHTTPNWNWNTTSGPNLNTTEGPWWNNTTAGPNWNTTEGPWWGNTTSGPNWNTTQGPWWNNTTQGPWWNNTTQGPWWNNTTEGPWWGNTTSGPNWNTTQGPWWNNTTQGPWWNSTTEGPWWNNTSAGPNWNTTEGPWWGNSTSGPNWNTTSGPWWNITTLGPNWNTTQWPNTNTTPNLTLSTPVPNWNTTQGPWGNNGTTLGPNWNTTVLEPQWNSTNGIPQNRTTTEGPQWNATTTEQPATTQENWWDKLMNEVGSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GB38</accession>
    <name>A0AAU9GB38_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Bromodomain adjacent to zinc finger domain protein 2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_03581</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WAL family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05503">
        <property type="entry name" value="Bromo_BAZ2A_B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15545">
        <property type="entry name" value="PHD_BAZ2A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000199">
        <property type="entry name" value="Bromodomain adjacent to zinc finger domain 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037374">
        <property type="entry name" value="BAZ2A/B_Bromo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
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            <begin position="1273"/>
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            <begin position="2654"/>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
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    <sequence length="3148" mass="335687" checksum="0E67BDD7482CD9C5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GB43</accession>
    <name>A0AAU9GB43_DROMD</name>
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        <recommendedName>
            <fullName evidence="21">Bromodomain adjacent to zinc finger domain protein 2B</fullName>
        </recommendedName>
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        <name evidence="19" type="ORF">DMAD_03581</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
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    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the WAL family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043471088" description="trypsin" evidence="13">
        <location>
            <begin position="17"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="14">
        <location>
            <begin position="31"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG05208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="256" mass="25775" checksum="C5A7AC156775D596" modified="2024-11-27" version="1">MLKFVILLSAVACALGGTIPEGLLPQLDGRIVGGSATTINSFPWQISLQRSGSHSCGGSVYSGNIIVTAAHCLQSVSASVLQVRAGSTYWSSGGTVAKVASFKNHEGYNANTMVNDIAVIRLSSSLGFSSTIKAIGLASSAPANGAAASVSGWGTESYGSSSIPTTLQYVNVNIVGQSQCASSTYAYGSEIKSSMICAASSGKDACQGDSGGPLVSGGVLVGVVSWGYGCAYANYPGVYADVAALRSWVVSAANSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GB90</accession>
    <name>A0AAU9GB90_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Microtubule-actin cross-linking factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_03839</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030056">
        <property type="term" value="C:hemidesmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21188">
        <property type="entry name" value="CH_PLEC-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21189">
        <property type="entry name" value="CH_PLEC-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000001">
        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1290.10:FF:000009">
        <property type="entry name" value="microtubule-actin cross-linking factor 1 isoform X13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1290.10:FF:000014">
        <property type="entry name" value="microtubule-actin cross-linking factor 1 isoform X21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1290.10:FF:000005">
        <property type="entry name" value="microtubule-actin cross-linking factor 1 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000110">
        <property type="entry name" value="Short stop, isoform AA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000048">
        <property type="entry name" value="Short stop, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1290.10:FF:000011">
        <property type="entry name" value="Short stop, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000031">
        <property type="entry name" value="Short stop, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000022">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000030">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000044">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000045">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000051">
        <property type="entry name" value="Short stop, isoform K"/>
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    </dbReference>
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        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000054">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000055">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000058">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000062">
        <property type="entry name" value="Short stop, isoform K"/>
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    <dbReference type="FunFam" id="1.20.58.60:FF:000064">
        <property type="entry name" value="Short stop, isoform K"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000082">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000045">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000038">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000042">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000047">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000050">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000057">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.20">
        <property type="entry name" value="Gas2-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1290.10">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041615">
        <property type="entry name" value="Desmoplakin_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003108">
        <property type="entry name" value="GAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036534">
        <property type="entry name" value="GAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049538">
        <property type="entry name" value="PCN-like_spectrin-like_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043197">
        <property type="entry name" value="Plakin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035915">
        <property type="entry name" value="Plakin_repeat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001101">
        <property type="entry name" value="Plectin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169">
        <property type="entry name" value="ENVOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169:SF23">
        <property type="entry name" value="SHORT STOP, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02187">
        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17902">
        <property type="entry name" value="SH3_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21019">
        <property type="entry name" value="Spectrin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21020">
        <property type="entry name" value="Spectrin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00243">
        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00250">
        <property type="entry name" value="PLEC"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143575">
        <property type="entry name" value="GAS2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75399">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51460">
        <property type="entry name" value="GAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 10" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="14">
        <location>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GB92</accession>
    <name>A0AAU9GB92_DROMD</name>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EUB6</accession>
    <name>A0AAU9EUB6_DROMD</name>
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        <recommendedName>
            <fullName evidence="8">dihydropyrimidinase</fullName>
            <ecNumber evidence="8">3.5.2.2</ecNumber>
        </recommendedName>
    </protein>
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        <name evidence="11" type="ORF">DMAD_08490</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5,6-dihydrouracil + H2O = 3-(carbamoylamino)propanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16121"/>
            <dbReference type="ChEBI" id="CHEBI:11892"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15901"/>
            <dbReference type="EC" id="3.5.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="9">Carbamylation allows a single lysine to coordinate two divalent metal cations.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.2" evidence="8"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EUB6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004157">
        <property type="term" value="F:dihydropyrimidinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006208">
        <property type="term" value="P:pyrimidine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01314">
        <property type="entry name" value="D-HYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000001">
        <property type="entry name" value="Dihydropyrimidinase like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011778">
        <property type="entry name" value="Hydantoinase/dihydroPyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050378">
        <property type="entry name" value="Metallo-dep_Hydrolases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02033">
        <property type="entry name" value="D-hydantoinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647:SF1">
        <property type="entry name" value="COLLAPSIN RESPONSE MEDIATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647">
        <property type="entry name" value="HYDRANTOINASE/DIHYDROPYRIMIDINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="10">
        <location>
            <begin position="72"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="9">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR611778-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF89822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="593" mass="64617" checksum="63914A9BACBF4579" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EUC0</accession>
    <name>A0AAU9EUC0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transforming acidic coiled-coil-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_08712</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TACC family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EUC0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1700:FF:000001">
        <property type="entry name" value="Transforming acidic coiled-coil-containing protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039915">
        <property type="entry name" value="TACC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007707">
        <property type="entry name" value="TACC_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13924">
        <property type="entry name" value="TRANSFORMING ACIDIC COILED-COIL CONTAINING PROTEIN 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13924:SF10">
        <property type="entry name" value="TRANSFORMING ACIDIC COILED-COIL PROTEIN, ISOFORM K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05010">
        <property type="entry name" value="TACC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transforming acidic coiled-coil-containing protein C-terminal" evidence="9">
        <location>
            <begin position="955"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="193"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="429"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="567"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="959"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1079"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="222"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="266"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="288"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="475"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="588"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF90130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1173" mass="130663" checksum="B080580765BFFF0E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EUD3</accession>
    <name>A0AAU9EUD3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Steroid transmembrane transporter SLC22A24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_09094</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9EUD3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="331"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="360"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="417"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="477"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="18"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Endopterygota</taxon>
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        </lineage>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
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            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
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            <end position="312"/>
        </location>
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            <begin position="238"/>
            <end position="254"/>
        </location>
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            <end position="312"/>
        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF89385.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EUI8</accession>
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            <fullName evidence="15">Phospholipid-transporting ATPase</fullName>
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        <name evidence="19" type="ORF">DMAD_08549</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="11 15">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
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        </reaction>
    </comment>
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        <cofactor evidence="14">
            <name>Mg(2+)</name>
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        </cofactor>
    </comment>
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        <subcellularLocation>
            <location evidence="1 15">Membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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    </dbReference>
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        <property type="component" value="Chromosome O"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0016887">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
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    </dbReference>
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        <property type="entry name" value="ATPASE_E1_E2"/>
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    </dbReference>
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    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
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            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
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            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
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            <end position="951"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
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            <end position="983"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
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            <end position="1062"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1117"/>
            <end position="1140"/>
        </location>
    </feature>
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        <location>
            <begin position="17"/>
            <end position="82"/>
        </location>
    </feature>
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            <end position="1145"/>
        </location>
    </feature>
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            <end position="1180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
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            <end position="1298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
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            <end position="1180"/>
        </location>
    </feature>
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        <location>
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            <end position="1257"/>
        </location>
    </feature>
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        <location>
            <position position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>ATP</name>
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    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="504"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="545"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="567"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="601"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="681"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="682"/>
        </location>
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                <person name="Yamamoto D."/>
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        <property type="entry name" value="RRM_dom"/>
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        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
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    <sequence length="498" mass="53633" checksum="F17D0B1EAC42973E" modified="2024-11-27" version="1">MLAMPTLMMPATAQMTLSSQPLSVGAKPYETKLLAIHQTHMQQQQQQQATSQQQQQNGKQQAQQMTIVTTQQQQQQQQQQQHPQSQSPHLQQQQHSQQQQQHSQQQAVAAAAAAAAAHHQQQQAVAAAVCAAQQQAVVVQQQQYQIHSQQQSPQQQVLSISPKQEQFHIFVGDLSSEIETQQLREAFTPFGEISDCRVVRDPQTLKSKGYGFVSFIKKSEAESAITAMNGQWLGSRSIRTNWATRKPPASKENIKPLTFDEVYNQSSPSNCTVYVGGVNSALTALSEEVLQKTFAPYGAIQEIRVFKDKGYAFVRFSTKEAATHAIVGVHNTELNAQPVKCSWGKESGDPNNAQTIATQALNSAAAAAAGFPYGLGATAAAAYGQQLAATGCWYSPTPTYQASSATATAVTPAAASAAAVQNQFLQGIQGYHFGQYGGYQQGYMGMGVQIPATWQGVTQAQISPAQQMATGVAGTAIPQAAGVVAYPIQQFQVSPQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6C4</accession>
    <name>A0AAU9F6C4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dual-specificity kinase</fullName>
            <ecNumber evidence="2">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_09329</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="2"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PKc_CLK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dual specificity protein kinase CLK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000061">
        <property type="entry name" value="Dual specificity protein kinase CLK2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CLK_kinases"/>
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    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="1295"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="93"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
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            <begin position="319"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
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            <begin position="644"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
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            <begin position="713"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="812"/>
            <end position="863"/>
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            <begin position="1005"/>
            <end position="1097"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1120"/>
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    </feature>
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        <location>
            <begin position="1186"/>
            <end position="1258"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1616"/>
            <end position="1643"/>
        </location>
    </feature>
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        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="15"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="194"/>
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        </location>
    </feature>
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        <location>
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        <location>
            <begin position="713"/>
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    </feature>
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        <location>
            <begin position="760"/>
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        <location>
            <begin position="848"/>
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        </location>
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            <begin position="1634"/>
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    <feature type="binding site" evidence="15">
        <location>
            <position position="1324"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <property type="entry name" value="tRNA_m1G_MTases_N"/>
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        <property type="match status" value="1"/>
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        <location>
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            <end position="1157"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF90916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1166" mass="133524" checksum="21D65B42F402CACF" modified="2024-11-27" version="1"> </sequence>
</entry>
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    <accession>A0AAU9F6D5</accession>
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        <recommendedName>
            <fullName evidence="8">Shal K[+] channel interacting protein</fullName>
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        <name evidence="6" type="ORF">DMAD_10033</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Diptera</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
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                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6 7">RF1</strain>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <location>
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            <end position="710"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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            <end position="195"/>
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            <end position="601"/>
        </location>
    </feature>
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        <location>
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            <end position="642"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="898"/>
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        </location>
    </feature>
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        <location>
            <begin position="30"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
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        </location>
    </feature>
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        <location>
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        <location>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF91835.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="954" mass="101578" checksum="C6A724C6CCF4EC1A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6F1</accession>
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        <submittedName>
            <fullName evidence="7">Isocitrate dehydrogenase</fullName>
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        <name evidence="7" type="ORF">DMAD_07729</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
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        <subcellularLocation>
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        </subcellularLocation>
    </comment>
    <comment type="similarity">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF60">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <location>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF88827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="393" mass="43634" checksum="097A404226CF7319" modified="2024-11-27" version="1">MALRLTQRLLQTQTPFLTRGYPMLVTKEKSEDVAHTKSSLQKKVTRTDIPSAQYGGRHAVTMLPGGGIGPELMTYVREIFSYCGAPIDFEVIEIDPSTEGNDDLDYAITSIKRNGVALKGNIETKSQELSEVSRNVAIRNELDLYVNVVHCKSYPGIPARHQDIDVVLIRQNTDGEYAMLEHESVPGIVESMKVVTVENAERVARYAFEYARQNNRKKVTTIHKANIMKLSDGLFLECAKRVHKDYPELEHDNMIIDNTCMQAVSKPHQFDVMNMTNLYGTIVSNVLCGLMGGAGLISGRNYGDHYAVFEPGTRNTGTAIAGKNIANPVAMINASIDMLNHLGHKEHANVIYEATYQTIVNDAIRTPDLGGNHSSTDVVENILKILSAKRVNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6G0</accession>
    <name>A0AAU9F6G0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Metalloendopeptidase</fullName>
            <ecNumber evidence="19">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_10076</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="18 19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="18 19">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="19"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008586">
        <property type="term" value="P:imaginal disc-derived wing vein morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032927">
        <property type="term" value="P:positive regulation of activin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030513">
        <property type="term" value="P:positive regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd04281">
        <property type="entry name" value="ZnMc_BMP1_TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000004">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000052">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000053">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000003">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000240">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015446">
        <property type="entry name" value="BMP_1/tolloid-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034036">
        <property type="entry name" value="ZnMP_TLD/BMP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF43">
        <property type="entry name" value="TOLLOID-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001199">
        <property type="entry name" value="BMP_1/tolloid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 18 19" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5 18 19" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="10 16 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="23">
        <location>
            <begin position="517"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="21">
        <location>
            <begin position="721"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="21">
        <location>
            <begin position="838"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="952"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="21">
        <location>
            <begin position="995"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="1112"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="21">
        <location>
            <begin position="1156"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="21">
        <location>
            <begin position="1269"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="64"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="243"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="388"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="388"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="404"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="424"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="active site" evidence="15 18">
        <location>
            <position position="613"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="612"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="616"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="622"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="582"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="584"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001199-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001199-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="BFF91910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1453" mass="163751" checksum="11B24482AD643A7B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6G9</accession>
    <name>A0AAU9F6G9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_08482</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057435">
        <property type="entry name" value="Lips"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37686">
        <property type="entry name" value="LD36006P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37686:SF1">
        <property type="entry name" value="LD36006P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25228">
        <property type="entry name" value="Lips"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="676"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="709"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="763"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1083"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1180"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1180"/>
            <end position="1208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF89813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1286" mass="144901" checksum="2843224D75333C95" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6H5</accession>
    <name>A0AAU9F6H5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Signal transducer and activator of transcription</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_09415</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="12">Forms a homodimer or a heterodimer with a related family member.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the transcription factor STAT family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F6H5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09919">
        <property type="entry name" value="SH2_STAT_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14786">
        <property type="entry name" value="STAT_CCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14801">
        <property type="entry name" value="STAT_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000095">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.630:FF:000003">
        <property type="entry name" value="Signal transducer and transcription activator 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.20">
        <property type="entry name" value="STAT transcription factor, all-alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.630">
        <property type="entry name" value="STAT transcription factor, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.532.10">
        <property type="entry name" value="STAT transcription factor, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001217">
        <property type="entry name" value="STAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048988">
        <property type="entry name" value="STAT_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036535">
        <property type="entry name" value="STAT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013800">
        <property type="entry name" value="STAT_TF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015988">
        <property type="entry name" value="STAT_TF_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013801">
        <property type="entry name" value="STAT_TF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012345">
        <property type="entry name" value="STAT_TF_DNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013799">
        <property type="entry name" value="STAT_TF_prot_interaction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11801">
        <property type="entry name" value="SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01017">
        <property type="entry name" value="STAT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02864">
        <property type="entry name" value="STAT_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02865">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21354">
        <property type="entry name" value="STAT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00964">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47655">
        <property type="entry name" value="STAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0010">Activator</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13 14" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 14" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SH2" evidence="16">
        <location>
            <begin position="584"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="731"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU046415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF91036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="774" mass="86848" checksum="CEA632910FD0A043" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6H7</accession>
    <name>A0AAU9F6H7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="7">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_10115</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F6H7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000092">
        <property type="entry name" value="Carboxylic ester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043089750" description="Carboxylic ester hydrolase" evidence="7">
        <location>
            <begin position="31"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="8">
        <location>
            <begin position="99"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF91955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="652" mass="73138" checksum="15DE6B3B9022681F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6H8</accession>
    <name>A0AAU9F6H8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SUEL-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_07795</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F6H8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22828">
        <property type="entry name" value="Gal_Rha_Lectin_EVA1_EVA1C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22829">
        <property type="entry name" value="Gal_Rha_Lectin_EVA1_EVA1C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46780">
        <property type="entry name" value="PROTEIN EVA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="411"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="3">
        <location>
            <begin position="88"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="3">
        <location>
            <begin position="217"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF88917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="670" mass="71645" checksum="2C15A3889C8B5B96" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6I0</accession>
    <name>A0AAU9F6I0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_08507</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F6I0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="437"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF89843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="627" mass="66847" checksum="63854352EC48EAD3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6I2</accession>
    <name>A0AAU9F6I2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="18 19 20">Poly [ADP-ribose] polymerase</fullName>
            <ecNumber evidence="4 19">2.4.2.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">DMAD_07808</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>L-aspartyl-[protein] + NAD(+) = 4-O-(ADP-D-ribosyl)-L-aspartyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:54424"/>
            <dbReference type="Rhea" id="RHEA-COMP:9867"/>
            <dbReference type="Rhea" id="RHEA-COMP:13832"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29961"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:138102"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 19">
            <text>L-glutamyl-[protein] + NAD(+) = 5-O-(ADP-D-ribosyl)-L-glutamyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:58224"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15089"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:142540"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 19">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 19">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.30" evidence="4 19"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF88938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F6I2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070212">
        <property type="term" value="P:protein poly-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17747">
        <property type="entry name" value="BRCT_PARP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01437">
        <property type="entry name" value="parp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08001">
        <property type="entry name" value="WGR_PARP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.142.10:FF:000001">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.10:FF:000006">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.10:FF:000002">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.142.10">
        <property type="entry name" value="Poly(ADP-ribose) polymerase, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.10">
        <property type="entry name" value="Zinc finger, PARP-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050800">
        <property type="entry name" value="ARTD/PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038650">
        <property type="entry name" value="PADR1_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008288">
        <property type="entry name" value="PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049296">
        <property type="entry name" value="PARP1-like_PADR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012982">
        <property type="entry name" value="PARP1-like_PADR1_Zn_ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004102">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036616">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036930">
        <property type="entry name" value="WGR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008893">
        <property type="entry name" value="WGR_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001510">
        <property type="entry name" value="Znf_PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036957">
        <property type="entry name" value="Znf_PARP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459:SF60">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21728">
        <property type="entry name" value="PADR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02877">
        <property type="entry name" value="PARP_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05406">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00645">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08063">
        <property type="entry name" value="Zn_ribbon_PADR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000489">
        <property type="entry name" value="NAD_ADPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01335">
        <property type="entry name" value="PADR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00773">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01336">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_PARP_2"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19 20" id="KW-0808">Transferase</keyword>
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    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="7"/>
            <end position="89"/>
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        <location>
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        </source>
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        </source>
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        </source>
    </evidence>
    <sequence length="993" mass="113233" checksum="47F5936998EAA98B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6I3</accession>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">RF1</strain>
        </source>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043549528" evidence="1">
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF91066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="259" mass="29000" checksum="B4539A9647F4D9EF" modified="2024-11-27" version="1">MAFQLFLVALLGIALVSATPVPQQAGSANFTSAMTRYLQAFRSVMPCGYAPEGIPVLAPIVTPFYAYDISTDDYSLKGNVSNMRIEGLNNFKILSASDYGKTGQAAYDVIFPKIQILGASEVEGFVNIGGFHLPIRQRDVINEALVDLRFVGSYTFANSLTNSSGLRIVDFKQSIYLADVLMDNWNSLWNISTNNFWNRYSRIFISLGLEEIQPKITDFLYQNYFVPKINELLANVSMEELVNFYQSQAQLWESAQCQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6I4</accession>
    <name>A0AAU9F6I4_DROMD</name>
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        <submittedName>
            <fullName evidence="6">Tight junction protein ZO-1</fullName>
        </submittedName>
    </protein>
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        <name evidence="6" type="ORF">DMAD_08517</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6 7">RF1</strain>
        </source>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cell-cell junction organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein localization to cell-cell junction"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11859">
        <property type="entry name" value="SH3_ZO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000029">
        <property type="entry name" value="tight junction protein ZO-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="tight junction protein ZO-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000179">
        <property type="entry name" value="Uncharacterized protein, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13865:SF28">
        <property type="entry name" value="POLYCHAETOID, ISOFORM O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13865">
        <property type="entry name" value="TIGHT JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00218">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="38"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="199"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="442"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="3">
        <location>
            <begin position="704"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="ZU5" evidence="5">
        <location>
            <begin position="2123"/>
            <end position="2256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFF94686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="5598" mass="598753" checksum="E343C031E263A9CF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FGC5</accession>
    <name>A0AAU9FGC5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trehalose 6-phosphate phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_12220</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Removes the phosphate from trehalose 6-phosphate to produce free trehalose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-trehalose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23420"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="EC" id="3.1.3.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Glycan biosynthesis; trehalose biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the trehalose phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.12" evidence="5"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FGC5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044651">
        <property type="entry name" value="OTSB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43768">
        <property type="entry name" value="TREHALOSE 6-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43768:SF3">
        <property type="entry name" value="TREHALOSE 6-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFF94634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="273" mass="31103" checksum="524E4A514865A052" modified="2024-11-27" version="1">MPEKRVAPVISRLEEFEQKLPGYLNSHNKLAILLDYDGTLAPIADNPSKTQMPVELEKTLHKLAKHPQIFLAVISGRGLKDVQAHVSINGTTYAGNHGLEIEYPDGSRHDYELPAEIKKNYTDMVRELKEQVEKNGAWVEDKRVSLTYHYRDTPVELKDAQKQLATEICLRHGFRANQAHEAIEAKPPVNWNKGEAALYILKQKFGDNWHKDVRVVFAGDDTTDEDAMRVLQGLGRSFRIATDEQIQTFADFRLPKQALMTDLLKWIAQVYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FGC7</accession>
    <name>A0AAU9FGC7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_12298</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FGC7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046964">
        <property type="entry name" value="RTN1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799:SF2">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="505"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="8">
        <location>
            <begin position="387"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="31"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="180"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="322"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="223"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF94750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="583" mass="62318" checksum="DE09749183E84366" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FGC9</accession>
    <name>A0AAU9FGC9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Protein split ends</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_12256</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM Spen family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12349">
        <property type="entry name" value="RRM2_SHARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12350">
        <property type="entry name" value="RRM3_SHARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12351">
        <property type="entry name" value="RRM4_SHARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21543">
        <property type="entry name" value="SPOC_SHARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000088">
        <property type="entry name" value="msx2-interacting protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000118">
        <property type="entry name" value="msx2-interacting protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.290.10:FF:000002">
        <property type="entry name" value="Spen family transcriptional repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000258">
        <property type="entry name" value="Split ends, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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                <person name="Yamamoto D."/>
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            <end position="184"/>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50913"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF94772.1"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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</entry>
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                <person name="Yamamoto D."/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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    <feature type="transmembrane region" description="Helical" evidence="11">
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        <location>
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        <location>
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            <end position="2201"/>
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    </feature>
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            <end position="1684"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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                <person name="Yamamoto D."/>
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            <fullName evidence="1 7">Hepatocyte growth factor-regulated tyrosine kinase substrate</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_12352</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Essential role in endosome membrane invagination and formation of multivesicular bodies, MVBs. Required during gastrulation and appears to regulate early embryonic signaling pathways. Inhibits tyrosine kinase receptor signaling by promoting degradation of the tyrosine-phosphorylated, active receptor, potentially by sorting activated receptors into MVBs. The MVBs are then trafficked to the lysosome where their contents are degraded.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FGJ8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15720">
        <property type="entry name" value="FYVE_Hrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21387">
        <property type="entry name" value="GAT_Hrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03569">
        <property type="entry name" value="VHS_Hrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1940:FF:000003">
        <property type="entry name" value="Hepatocyte growth factor-regulated tyrosine kinase substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000014">
        <property type="entry name" value="Hepatocyte growth factor-regulated tyrosine kinase substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017073">
        <property type="entry name" value="HGS/VPS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024641">
        <property type="entry name" value="HRS_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46275">
        <property type="entry name" value="HEPATOCYTE GROWTH FACTOR-REGULATED TYROSINE KINASE SUBSTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46275:SF1">
        <property type="entry name" value="HEPATOCYTE GROWTH FACTOR-REGULATED TYROSINE KINASE SUBSTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12210">
        <property type="entry name" value="Hrs_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036956">
        <property type="entry name" value="Hrs_Vps27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VHS" evidence="12">
        <location>
            <begin position="13"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="11">
        <location>
            <begin position="158"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="301"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="342"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="575"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="441"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="353"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="617"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="653"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="674"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="720"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00015450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF94824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="741" mass="82747" checksum="55A2C4EE0602B290" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FP53</accession>
    <name>A0AAU9FP53_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">cAMP-dependent protein kinase type I regulatory subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_05832</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF97410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007611">
        <property type="term" value="P:learning or memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000006">
        <property type="entry name" value="cAMP-dependent protein kinase type I-alpha regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
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        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
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        <property type="entry name" value="cNMP-bd_CS"/>
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        <property type="entry name" value="cNMP-bd_dom"/>
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    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0114">cAMP</keyword>
    <keyword evidence="3 7" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="5 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="9">
        <location>
            <begin position="77"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="9">
        <location>
            <begin position="195"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF97410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="322" mass="35625" checksum="070092F87117ED62" modified="2024-11-27" version="1">MPKEQVKLDASKQVISPDDCDDLSPMPQTAAPPVRRRGGISAEPVTEEDASNYVKKVVPKDYKTMNSLSKAIAKNVLFAHLDESERSDIFDAMFPVNHIAGENIIQQGDEGDNFYVIDVGEVDVFVNSELVTTISEGGSFGELALIYGTPRAATVRAKSDVKLWGIDRDSYRRILMGSTIRKRKMYEEFLSRVSILESLDKWERLTVADSLETCSFDDGETIVKQGAAGDDFYIILEGCAVVLQQRSEQGEDPAEVGRLGSSDYFGEIALLLDRPRAATVVARGPLKCVKLDRARFERVLGPCADILKRNITQYNSFVSLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FP54</accession>
    <name>A0AAU9FP54_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Lon protease homolog, mitochondrial</fullName>
            <ecNumber evidence="11">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_05623</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homohexamer or homoheptamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12 13">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="11"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FP54"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070407">
        <property type="term" value="P:oxidation-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000043">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5270:FF:000001">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1480:FF:000002">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000015">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03120">
        <property type="entry name" value="lonm_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027503">
        <property type="entry name" value="Lonm_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718">
        <property type="entry name" value="LON PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718:SF2">
        <property type="entry name" value="LON PROTEASE HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 11 12" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="16">
        <location>
            <begin position="95"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="15">
        <location>
            <begin position="775"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="221"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="985"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="221"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="active site" evidence="11 12">
        <location>
            <position position="879"/>
        </location>
    </feature>
    <feature type="active site" evidence="11 12">
        <location>
            <position position="922"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="539"/>
            <end position="546"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF97145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1006" mass="112698" checksum="C83642069D10134B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FP57</accession>
    <name>A0AAU9FP57_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 4H</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_05991</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF97613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FP57"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
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            <end position="143"/>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="266" mass="29684" checksum="679546F9FA320692" modified="2024-11-27" version="1">MSKSHSYKKSKSSSSAGSLEDFLSVTKLRISDEDKEDSSCQSLDAISEQSAPSEKLPQVDVPLHPPFVALIGNLPMESSERELRQLFAQHTIRSVSMLQRGKRQRQHGSAQVELQTRQDLLELLKKNQLLCRGRRLSIAVCQDLGAGGDDCWSMNSDGRWSRRSASGGRHSGSDADESCSEYSLPSRGEESTSSSLHISRKPSSNEELERRMQIRLQKLAEFENAQTERLQAQQQSSQGEDGEAPSVPSMSWPDFWAEVQKSEDLE</sequence>
</entry>
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        <name type="scientific">Drosophila madeirensis</name>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        </location>
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    </feature>
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            <end position="230"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF97729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="466" mass="52073" checksum="9B0B1E48134F98DA" modified="2024-11-27" version="1">MSDYRSQSRGGGRGGQDYSNDDDPPMSRLFIICNKAHTEDDFREAFSAYGDIEDIWVVKDKHTQENKGIAYVKFSKTSDAAKAQEEMNGKTIGKMDRTLKVLVAANRNQGSNKSENEQEKYVRLFIVIPKTATEDDIRDEFAQWGEVESVTIVREKNNGNPKGFGYVRFTKFYYAAVAFENCSPKYKAVFAEPKGSTRSQRDQYGRPAEDNPVYSNPGRGGSSYNNNGASSGAGSFNNDWNTSTNNDMSAFLRMQNVPVAQPSCLEVTVSNCVNQDQLWRLFDIIPGLDYCQIMREHGPRTNEAMVVYDNPEAAIYAKDKLHGLEYPMGERIIVKVNGMSSARMDTSFIDKRTKKDAICNVPLPPAQPLASPDAQVAQRLFIVLSANLPHSILKNIFSCWKGLIDVYLLPNKNCGYVKYAECESAQRAIHVLNGAEICGTKIKVMEAEERSGSDDGDGGRKRLRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FP62</accession>
    <name>A0AAU9FP62_DROMD</name>
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        <recommendedName>
            <fullName evidence="6">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_05839</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DUF7805"/>
    </dbReference>
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        <property type="entry name" value="LDL_receptor-like_sf"/>
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        <property type="entry name" value="LDLR_class-A_CS"/>
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        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053207">
        <property type="entry name" value="Non-NMDA_GluR_Accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47537">
        <property type="entry name" value="CUBILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47537:SF4">
        <property type="entry name" value="GH12701P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25090">
        <property type="entry name" value="DUF7805"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
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        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043627953" description="CUB domain-containing protein" evidence="5">
        <location>
            <begin position="46"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1114"/>
            <end position="1134"/>
        </location>
    </feature>
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        <location>
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            <end position="250"/>
        </location>
    </feature>
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            <end position="532"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
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            <end position="74"/>
        </location>
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        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
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            <end position="64"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF97422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1175" mass="131620" checksum="469759EEAD44BC28" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXE0</accession>
    <name>A0AAU9FXE0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Serine-rich adhesin for platelets</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_00198</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Rough endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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                <person name="Yamamoto D."/>
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    </evidence>
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</entry>
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                <person name="Yamamoto D."/>
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    <sequence length="256" mass="28634" checksum="F99B00CD56B0688A" modified="2024-11-27" version="1">MSTAGEDYLFSRQFRELSIRRRAGGLLVIQFQRWQRRTVYELMRALDAANADEDISIVVLSGEFAVGPEGDCQQQPLALEQGKGSEKRDEAEDRFIQHRAADFVMRSLAKKLLGFRKLLVAFVQGPCQGLGVSICSLCDLVYATETGHFLLSLSHLHPCVEEGPSWSLPQIELLLRLGERADARCAWGSGFVASLTADAQEFWTRMDQYSRLPTVSLLATKRLLLRPGRDTLLAQLRQEGTPLAAQRRRIAASSKL</sequence>
</entry>
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            <authorList>
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                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <end position="225"/>
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        <source>
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        </source>
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        </source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG00080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="277" mass="29657" checksum="C751FD36C6264E38" modified="2024-11-27" version="1">MDNSIVVEENGNSASSGSLDVITAAAQAAAAAAAATSAAQQQSQNPQSTTAASAGNTNNSAGTATVLTTTANCNIQYPIQALTQHGLQVQSVIQANPSGVIQTAAGTQQHQALAAATASAMHKGVGVVYVAKPPNSTVIHTTAGNAVQVRNKIPPTFPCKIKPEPNSQHQEDSDESLSDDDSQHHRSELTRRPSYNKIFTEISGPDISDNSGIAEDQTRKREIRLQKNREAARECRRKKKEYIKCLENRVAVLENQNKALIEELKSLKELYCQTKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXF5</accession>
    <name>A0AAU9FXF5_DROMD</name>
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        <submittedName>
            <fullName evidence="3">Extensin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_00205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome A"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043874344" evidence="2">
        <location>
            <begin position="28"/>
            <end position="628"/>
        </location>
    </feature>
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        <location>
            <begin position="38"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="593"/>
        </location>
    </feature>
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        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
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        <location>
            <begin position="355"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="426"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="572"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG00142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="628" mass="65166" checksum="446C2A293838E864" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXG2</accession>
    <name>A0AAU9FXG2_DROMD</name>
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        <submittedName>
            <fullName evidence="15">Serine protease persephone</fullName>
        </submittedName>
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        <name evidence="15" type="ORF">DMAD_00171</name>
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    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase S1 family. CLIP subfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome A"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Complement C1r subcomponent"/>
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    </dbReference>
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        <property type="entry name" value="Serine protease easter"/>
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    </dbReference>
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        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF135">
        <property type="entry name" value="CLIP DOMAIN-CONTAINING SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00680">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51888">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043684106" evidence="12">
        <location>
            <begin position="21"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="Clip" evidence="14">
        <location>
            <begin position="25"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="13">
        <location>
            <begin position="350"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="85"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="180"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="215"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG00095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="611" mass="65945" checksum="853490A0710E41D5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXG7</accession>
    <name>A0AAU9FXG7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Nuclear pore complex protein Nup153</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">153 kDa nucleoporin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Nucleoporin Nup153</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_00744</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the NUP153 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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</entry>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FXJ1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050621">
        <property type="entry name" value="Tudor_domain_containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948:SF29">
        <property type="entry name" value="FI02030P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948">
        <property type="entry name" value="TUDOR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tudor" evidence="3">
        <location>
            <begin position="69"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="380"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="395"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG00276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="442" mass="51867" checksum="4D622B83AB16B820" modified="2024-11-27" version="1">MLDQKLRRLPMGNVAGREGKLKILITNVFSPLQFWFQFVESNNKYNLEGLHSKMSEFYAWHQAPIIPGTLLPGSICAAASKWVGWRRVRILSEPPDNATHVSVFYLDYGCQESIACKDLRFLPKMFTDIPAMAVRGALSRVHPLEYMWPGEATMMFRQKVMFGVTYAQIIEEDLQEAIYYVEIYPTKKTGALSVNSLMIQNKLAGNGAELQFENHCDRRLCYVQERLPSFEMLETGAFPMDADREFQKYFRGIVCTASFVMEFKVPHTTNPFLQDLTQALLDWMIGFKKKQIEQSRWGAMELENELENELERDMIEWGDDEEELQMVKNKEEPEQTIDPGQLLKAKEKEELEKDMQELKELMELDNFTEQEELREKYLQDIKEDPEQATDEEELQMVKKKEEPEQANEQEKLLKELQQAKEYGKMFKEMYKEMYNELSKVHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXK1</accession>
    <name>A0AAU9FXK1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Casein kinase II subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_00768</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Participates in Wnt signaling. Plays a complex role in regulating the basal catalytic activity of the alpha subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FXK1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1820.10:FF:000001">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000002">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01101">
        <property type="entry name" value="CK2_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG00865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="229" mass="26353" checksum="88A331F6982D5ED4" modified="2024-11-27" version="1">MSSSEEVSWVTWFCGLRGNEFFCEVDEDYIQDKFNLTGLNEQVPNYRQALDMILDLEPEDELEDNPLQSDMTEQAAEMLYGLIHARYILTNRGIAQMIEKYQTGDFGHCPRVYCESQPMLPLGLSDIPGEAMVKTYCPKCIDVYTPKSSRHHHTDGAYFGTGFPHMLFMVHPEYRPKRPTNQFVPRLYGFKIHSLAYQIQLQAAANFKMPLRAFATGRPIDNKKNNTAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXK5</accession>
    <name>A0AAU9FXK5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Disks large 1 tumor suppressor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_00552</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043297">
        <property type="term" value="P:apical junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009792">
        <property type="term" value="P:embryo development ending in birth or egg hatching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097120">
        <property type="term" value="P:receptor localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06723">
        <property type="entry name" value="PDZ1_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06724">
        <property type="entry name" value="PDZ2_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06795">
        <property type="entry name" value="PDZ3_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11861">
        <property type="entry name" value="SH3_DLG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001402">
        <property type="entry name" value="Discs, large homolog 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.470:FF:000001">
        <property type="entry name" value="Disks large 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000058">
        <property type="entry name" value="Disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000048">
        <property type="entry name" value="disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000049">
        <property type="entry name" value="disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.470">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015143">
        <property type="entry name" value="L27_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
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        </source>
    </evidence>
    <sequence length="1352" mass="148317" checksum="F2B48C8ECB570512" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXL5</accession>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13 14">RF1</strain>
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        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG00880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="219" mass="25298" checksum="54E59C6D55F85B8D" modified="2024-11-27" version="1">MKEFHKIRRQAAISAASASFTCVECDMELPRLRLLDEHMLKVHGGPACVVCKRRYKTRHELRRHQLRHNSERNVECSHPGCGKRFFTNRHMRNHTKVHTQEKNFVCENCGYSCRNKETLRVHIRSHTGERPFGCTVCDKRFPSHSGLREHMAMHSTDRPFVCNVCDATFSRQKGLYHHKFLHTDTKQFVCKLCGNAYAQAAGLAGHMRKHRSEGQNGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXL7</accession>
    <name>A0AAU9FXL7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP-1 complex subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_00447</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FXL7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000030">
        <property type="entry name" value="AP-1 complex subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017107">
        <property type="entry name" value="AP1_complex_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037094">
        <property type="entry name" value="AP1_complex_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GAE" evidence="11">
        <location>
            <begin position="834"/>
            <end position="944"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG00448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="952" mass="103788" checksum="8CAFF5F1400D8C3F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXL8</accession>
    <name>A0AAU9FXL8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Voltage-gated potassium channel subunit beta-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_00244</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FXL8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19142">
        <property type="entry name" value="AKR_AKR6B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000032">
        <property type="entry name" value="Hyperkinetic, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005983">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01293">
        <property type="entry name" value="Kv_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150:SF2">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01577">
        <property type="entry name" value="KCNABCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="11">
        <location>
            <begin position="264"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="68"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="155"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG00202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="594" mass="64544" checksum="8D5078A26E90F292" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0T3</accession>
    <name>A0AAU9G0T3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Sushi von Willebrand factor type A EGF and pentraxin domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_01274</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G0T3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000104">
        <property type="entry name" value="Complement component 4 binding protein beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000076">
        <property type="entry name" value="Furrowed, isoform A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000095">
        <property type="entry name" value="Furrowed, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000141">
        <property type="entry name" value="Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000105">
        <property type="entry name" value="Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006585">
        <property type="entry name" value="FTP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46393:SF7">
        <property type="entry name" value="COMPLEMENT C2"/>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15 16">RF1</strain>
        </source>
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        <subcellularLocation>
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    <comment type="caution">
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    <feature type="disulfide bond" evidence="10">
        <location>
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    <feature type="disulfide bond" evidence="10">
        <location>
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    <feature type="disulfide bond" evidence="10">
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG01543.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0U7</accession>
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            <fullName evidence="8">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_01255</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
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            <topology evidence="8">Multi-pass membrane protein</topology>
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        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG01518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="997" mass="113962" checksum="39753E56D499F430" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0V1</accession>
    <name>A0AAU9G0V1_DROMD</name>
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        <submittedName>
            <fullName evidence="3">Protein snakeskin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_01178</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G0V1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019991">
        <property type="term" value="P:septate junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056677">
        <property type="entry name" value="DUF7775"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038976">
        <property type="entry name" value="Ssk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36692:SF2">
        <property type="entry name" value="GEO12064P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36692">
        <property type="entry name" value="PROTEIN SNAKESKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24985">
        <property type="entry name" value="DUF7775"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7775" evidence="2">
        <location>
            <begin position="7"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG01426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="125" mass="13449" checksum="51570B8B567E607C" modified="2024-11-27" version="1">MEFNNRLLLKIIELAIAIACIVMYEVDGALSSRPLIVCGTIGGFTIICGVLLIGHVINSLVEKRLNALISLIGCILFVASGALVIDEWHDAVLQKDRKRNAIAAGSLMIINGAVFLLDTLSICRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0V5</accession>
    <name>A0AAU9G0V5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Blood vessel epicardial substance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_01419</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the popeye family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G0V5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051146">
        <property type="term" value="P:striated muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006916">
        <property type="entry name" value="POPDC1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055272">
        <property type="entry name" value="POPDC1-3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12101:SF1">
        <property type="entry name" value="BVES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12101">
        <property type="entry name" value="POPEYE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04831">
        <property type="entry name" value="POPDC1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="POPDC1-3" evidence="8">
        <location>
            <begin position="102"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="416"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG01739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="428" mass="46787" checksum="40C1EAD04861CCA4" modified="2024-11-27" version="1">MPSTAGSAASVGALINSAGSSASVMGIGAGGGSGVGTGVAAVAAAEVGAGAAAAASAGTLIAQSTAGTSAASGTITWDNNGTLRSINPGDWSIEQCLNWQQPHHLYFQLGWAFLFLAFLAPHGPFGSLWMRAMLLIGCIMMGMYGYLVECTPDVVLWSGLGIGVNFIYLIVVLCRLRPVRFEQEIEAVYMALFQPLHVTRHQFKKVLNCMKVIRALKYQEVYAQEKVTKVDSLSLVLSGKLVVSQHQRALHIVFPHQFLDSPEWFGVSTDDYFQVSIMAMEESRVLIWHRDKLKLSIMAEPFLQTVFDHILGRDVVKKLMQVTQVSESIASNGFLPSGGYAEDAEDKPMLILKKSVDVGHGLTALINRQLQEEHVPLLGRTYKQQLQLQLQQEQLQLQHQLQMQLQLQPEPRVHESSSTANNNEQSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0Y0</accession>
    <name>A0AAU9G0Y0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_01046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G0Y0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG01250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="296" mass="34433" checksum="5BA4A7893BC9175E" modified="2024-11-27" version="1">MNCNIHHHHQHQHQHHQHHHREEEYEYDEEEEDDKTDDDDDDDDDDDDDDNGNDDDDDEYGISRTAICRHCQRPQWEVLQRPIRQCRSRSKSTSKSRKRSKSRKRQQNHQRTRQHQHQHQHQHQHQQPRWPELNVANEQAIRFRCPQVGPLVGRDLHTPQPKHFHHPQLQSQQQSQLQLQVPQQFVPKKSKRKSRQLQQQQQQQQQQQQQKEGTEQTTRSPAHFQSRVAAVGGAQVAQLPLPPLSVIVGGPEAGEAEALGEGEGVGEEQQLPHTPDSPSLVTVSTWYTVAKQGVSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0Y2</accession>
    <name>A0AAU9G0Y2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">High mobility group protein DSP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_01291</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HMGB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G0Y2"/>
    <dbReference type="SMR" id="A0AAU9G0Y2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21978">
        <property type="entry name" value="HMG-box_HMGB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01390">
        <property type="entry name" value="HMG-box_NHP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000016">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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        <name evidence="7" type="ORF">DMAD_01106</name>
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        <name type="scientific">Drosophila madeirensis</name>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="BFG01332.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1427" mass="155509" checksum="42DA7BFFEE039A63" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0Z1</accession>
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        <recommendedName>
            <fullName evidence="3">histone acetyltransferase</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="34" type="ORF">DMAD_01467</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="34 35">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
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            <text>(S)-lactoyl-CoA + L-lysyl-[protein] = N(6)-[(S)-lactoyl]-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61996"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:19466"/>
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            <dbReference type="ChEBI" id="CHEBI:231527"/>
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        </reaction>
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            <dbReference type="Rhea" id="RHEA:61997"/>
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            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:57288"/>
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        </reaction>
    </comment>
    <comment type="subcellular location">
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            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="BFG01531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G158"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16442">
        <property type="entry name" value="RING FINGER PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16442:SF1">
        <property type="entry name" value="RING FINGER PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tudor" evidence="1">
        <location>
            <begin position="383"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="1">
        <location>
            <begin position="587"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG01531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="704" mass="79706" checksum="4EB10C17B502E43C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G160</accession>
    <name>A0AAU9G160_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Diacylglycerol kinase</fullName>
            <shortName evidence="13">DAG kinase</shortName>
            <ecNumber evidence="13">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_01172</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="13"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G160"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010646">
        <property type="term" value="P:regulation of cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043052">
        <property type="term" value="P:thermotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20802">
        <property type="entry name" value="C1_DGK_typeIV_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20855">
        <property type="entry name" value="C1_DGK_typeIV_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000204">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000012">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000020">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056383">
        <property type="entry name" value="DGKI-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF80">
        <property type="entry name" value="EYE-SPECIFIC DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23578">
        <property type="entry name" value="DGKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DAGKc" evidence="15">
        <location>
            <begin position="395"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="958"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="994"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="327"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="852"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="365"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG01417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1062" mass="117816" checksum="161D596B839552F8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G163</accession>
    <name>A0AAU9G163_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Coronin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_01105</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">F-actin regulator involved in anterograde Golgi to endosome transport: upon ubiquitination via 'Lys-33'-linked ubiquitin chains by the BCR(KLHL20) E3 ubiquitin ligase complex, interacts with EPS15 and localizes to the trans-Golgi network, where it promotes actin polymerization, thereby facilitating post-Golgi trafficking. May play a role in the maintenance of the Golgi apparatus morphology.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the WD repeat coronin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G163"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000076">
        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000362">
        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015505">
        <property type="entry name" value="Coronin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015048">
        <property type="entry name" value="DUF1899"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856:SF20">
        <property type="entry name" value="CORONIN-7"/>
        <property type="match status" value="1"/>
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            <fullName evidence="10">Protein spinster</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_04033</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G9F3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17328">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF79">
        <property type="entry name" value="PROTEIN SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="407"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="541"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="96"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="43"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG05258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="591" mass="64324" checksum="AD4B5CEC18F6C33A" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAU9G9F5_DROMD</name>
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        <submittedName>
            <fullName evidence="14">Probable ascorbate-specific transmembrane electron transporter 1</fullName>
        </submittedName>
    </protein>
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        <name evidence="14" type="ORF">DMAD_03511</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
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        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Cytochrome b reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CYB561/CYBRD1-like"/>
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        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106:SF24">
        <property type="entry name" value="NO EXTENDED MEMORY, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="171"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="13">
        <location>
            <begin position="65"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG04573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="291" mass="32645" checksum="01E475DCD21D92DD" modified="2024-11-27" version="1">MDSNSVSPLPPLEAPLAMLDSEKTPPGTPNGIEMPPPPDEKRYEEDPDNAWNCCSWCEYLLIVILSTILLVGVLVLTLFWVMFYRDGFAWSESPKQQFNLHPILMIAGFVTLSGFSILIYRLCRCVKHIYVKLIHMFFHAIAIPCIALGFLSVFDSHEALQKVNFYSLHSWLGFVTMGMFVLQFVIGFFSFLVMLCCENKTYSCRSAMVPIHASLGLANFWLAIATSVTGLIEKERETVAETNVSAENKLIEHYITSAIGITLIFIGIIVTFAVRRSNAPASAKVYVTERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G9G2</accession>
    <name>A0AAU9G9G2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidoglycan-recognition protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_03283</name>
        <name evidence="14" type="ORF">DMAD_03286</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 15">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFG04272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G9G2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042834">
        <property type="term" value="F:peptidoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.80.10:FF:000001">
        <property type="entry name" value="Peptidoglycan recognition protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="entry name" value="Peptidoglycan recognition protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017331">
        <property type="entry name" value="Peptidoglycan_recognition"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015510">
        <property type="entry name" value="PGRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006619">
        <property type="entry name" value="PGRP_domain_met/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022">
        <property type="entry name" value="PEPTIDOGLYCAN RECOGNITION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022:SF75">
        <property type="entry name" value="PEPTIDOGLYCAN-RECOGNITION PROTEIN SB1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037945">
        <property type="entry name" value="PGRPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00644">
        <property type="entry name" value="Ami_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00701">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="N-acetylmuramoyl-L-alanine amidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 8" id="KW-0391">Immunity</keyword>
    <keyword evidence="4 8" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044713103" description="Peptidoglycan-recognition protein" evidence="10">
        <location>
            <begin position="22"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidoglycan recognition protein family" evidence="12">
        <location>
            <begin position="22"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="11">
        <location>
            <begin position="37"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="58"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR037945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037945-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG04272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFG04276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="185" mass="20360" checksum="627B0E74324843FC" modified="2024-11-27" version="1">MVSKVALLLAVLVCSQYMAQGVYVISKGEWGGRAPSWTVALGNYLGYAIIHHTAGSYCETRSACEAVLRSVQNYHMDALGWPDIGYNFLIGGDGNVYEGRGWNRMGAHAAEWNPYSIGISFLGNFNWDTLEPNMIAAAKNLLNDAVNRGQLSSGYILYGHRQVSATECPGTHIWNEIRGWSHWSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G9G9</accession>
    <name>A0AAU9G9G9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dystrobrevin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_04017</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the dystrophin family. Dystrobrevin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G9G9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016010">
        <property type="term" value="C:dystrophin-associated glycoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046716">
        <property type="term" value="P:muscle cell cellular homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099536">
        <property type="term" value="P:synaptic signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16244">
        <property type="entry name" value="EFh_DTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02334">
        <property type="entry name" value="ZZ_dystrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000014">
        <property type="entry name" value="Dystrobrevin alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017432">
        <property type="entry name" value="Distrobrevin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015153">
        <property type="entry name" value="EF-hand_dom_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015154">
        <property type="entry name" value="EF-hand_dom_typ2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050774">
        <property type="entry name" value="KCMF1/Dystrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268:SF27">
        <property type="entry name" value="DYSTROBREVIN, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09068">
        <property type="entry name" value="EF-hand_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09069">
        <property type="entry name" value="EF-hand_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038204">
        <property type="entry name" value="Distrobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="12">
        <location>
            <begin position="229"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="511"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="611"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="430"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="516"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="568"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="622"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG05230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="694" mass="77058" checksum="F511BB82245B4B92" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G9I0</accession>
    <name>A0AAU9G9I0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Rho guanine nucleotide exchange factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_03647</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20832">
        <property type="entry name" value="C1_ARHGEF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13329">
        <property type="entry name" value="PH_RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08756">
        <property type="entry name" value="RGS_GEF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Yamamoto D."/>
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</entry>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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                <person name="Yamamoto D."/>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
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        <location>
            <begin position="636"/>
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        </location>
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        <location>
            <begin position="658"/>
            <end position="671"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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            <begin position="868"/>
            <end position="879"/>
        </location>
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        </location>
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        </location>
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        </location>
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            <begin position="1106"/>
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        </location>
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        <location>
            <begin position="1140"/>
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        <location>
            <begin position="1167"/>
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            <begin position="1213"/>
            <end position="1222"/>
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            <begin position="1245"/>
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        <location>
            <begin position="1344"/>
            <end position="1354"/>
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        <location>
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            <end position="1366"/>
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            <begin position="1403"/>
            <end position="1436"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG05798.1"/>
        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAY4</accession>
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        <recommendedName>
            <fullName evidence="11">Prominin</fullName>
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    </protein>
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        <name evidence="9" type="ORF">DMAD_04334</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prominin family.</text>
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    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome E"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008795">
        <property type="entry name" value="Prominin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11238">
        <property type="entry name" value="PROMININ ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11238:SF9">
        <property type="entry name" value="PROMININ, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05478">
        <property type="entry name" value="Prominin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="479"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="515"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="834"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="869"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1077"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1164"/>
            <end position="1222"/>
        </location>
    </feature>
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        <location>
            <begin position="877"/>
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        </location>
    </feature>
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        </location>
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        </location>
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            <end position="992"/>
        </location>
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            <end position="1027"/>
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            <end position="1095"/>
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    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1202"/>
            <end position="1213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG05642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1222" mass="136689" checksum="C6F376A6C4238EB8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAY5</accession>
    <name>A0AAU9GAY5_DROMD</name>
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        <recommendedName>
            <fullName evidence="20">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_03548</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel HCN family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000049">
        <property type="entry name" value="Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013621">
        <property type="entry name" value="Ion_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08412">
        <property type="entry name" value="Ion_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0114">cAMP</keyword>
    <keyword evidence="6" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0915">Sodium</keyword>
    <keyword evidence="4" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="14" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="751"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="830"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="939"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1014"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="20">
        <location>
            <begin position="1116"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="187"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="315"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="388"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="446"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="505"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1285"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="61"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="86"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
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            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="473"/>
            <end position="482"/>
        </location>
    </feature>
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        <location>
            <begin position="512"/>
            <end position="525"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="BFG04629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1320" mass="147821" checksum="62DD2711C598F124" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAY6</accession>
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        <submittedName>
            <fullName evidence="7">Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha</fullName>
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        <name evidence="7" type="ORF">DMAD_03540</name>
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    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
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        </subcellularLocation>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="2-Layer Sandwich"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG04616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="843" mass="93504" checksum="2219970CFA54BB27" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GB61</accession>
    <name>A0AAU9GB61_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_03818</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the UPF0598 family.</text>
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        <property type="protein sequence ID" value="BFG04971.1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14956">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG04971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="217" mass="25321" checksum="A561102B6A6D49DB" modified="2024-11-27" version="1">MIKIQPRHLINLKNVGFNLLSTRNLHYVQGQSPEPKIREYFYYIDHEGMLFLDDARMKNFTSCFKEKAFLKFFFSRLRLNKTQRYLSDFPYISLCGRERNFIRCDDTPLVFTEQLKKTDGGEEVLGYAHAGPVLTLPFEPHKLYMDPKNGRVYHPATPQVGGIGLVRSKLAIELSQHFEFPAGDASPTHFKWHGERLKLENDWVTGTQRFPMSEECQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GB68</accession>
    <name>A0AAU9GB68_DROMD</name>
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        <submittedName>
            <fullName evidence="4">Phosphatidylinositol 4-5-bisphosphate 5-phosphatase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_03651</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP001500889">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF275">
        <property type="entry name" value="LD06095P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043773417" evidence="2">
        <location>
            <begin position="22"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="3">
        <location>
            <begin position="8"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG04775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="346" mass="39320" checksum="6C0EF27826DC1E0E" modified="2024-11-27" version="1">MVATTAIADLCIFLLTWNVGTHSPRDLSLTSLLALNGTTNCPDNQLPDIYVIGMQEVSTKQVLNIFQDDPWVLKIASHLQDHQYVKVDSKQLQGILITMFAQHKHIPHMKNIETEATRTGLGGLWGNKGAVSIRLSLYGTGAVFVCSHLAAHDDKLKERIEDYHQIVDNHKYEASGYRRIFDHDFVFWFGDLNFRLSGETSAWDVRTAAENMRYEELLKQDQLNLLRETGNAFSLLEEQPPTFAPTFKFIEGTNDYDLKRRPAWCDRILHRVQRNIYPAVTLSATQLSYQSNMEYTLSDHKPVSATFNYKVETANQTDEELHEMTHAGAATAAGTPNAKSVKRKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GB69</accession>
    <name>A0AAU9GB69_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_04549</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GB69"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="128"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="321"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="361"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG05933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="460" mass="51784" checksum="3D211DB4D58FE467" modified="2024-11-27" version="1">MGNDDLRRRKTPCGFCMAVFKWIPVVFITAVIAWSYYAYVVELCIRNAENLVGMIFMLFFYHIALILFMWSYWRTIMTSVGRVPDQWRIPDEEVTRLFRADNPETQKRILNSFARSLPVTNRTMNGSVRFCEKCKIIKPDRAHHCSVCNSCVLKMDHHCPWVNNCVNFYNYKFFVLFLGYALVYCLYVAFTTLHDFVQFWKVGAGQLNGSGVGRFHILFLFFISIMFAISLVSLFGYHIYLVLVNRTTLESFRAPVFRVGGPDKNGFNLGRYANFCEVFGDDWQYWPLPIFTSRGDGFSYPTSTDQSGEAPTVQRYAAMGDTTTNRLDGNPTDKLIDAIPLDTTPNNHDQPPPQHQHPHQVRSQNQAQPQPQQARHQARPNSLNLQPALELPLANGQSSNCSTAAVPSSQMVVVNLGTLPEDANGSAVIIDMVGDHSGAAAGVAAPKDACRQPNGDVPLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GB70</accession>
    <name>A0AAU9GB70_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc carboxypeptidase A 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_03968</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the digestion of the blood meal.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GB70"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03860">
        <property type="entry name" value="M14_CP_A-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.340:FF:000002">
        <property type="entry name" value="Carboxypeptidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000040">
        <property type="entry name" value="zinc carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF60">
        <property type="entry name" value="FI16720P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 17" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M14" evidence="16">
        <location>
            <begin position="127"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="15">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG05173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="428" mass="48312" checksum="DA9329F795E60292" modified="2024-11-27" version="1">MAASGIKLESGDGKCLLDAGTAPPADLARYDHYRIYNVQFDNQEQIELFQKLEEQSDSLTFIGHAREIGQKLSILVAAHRVADFADLLKTYKLKHRVLTYNFQEKIDRNMGEVLPESIDATKYDWQHFFHLKTIYGWMDAMAKQYPNLLTVLDMGNSTQGHAIKGLKLSSNPENKAIFIESGIHAREWIAPATATYIINELLTSQDAGVQGLAKNYNWIIFPCVNPDGYKYTFEHDRMWRKNRQLFGTCRGVDLNRNYPDHWNSTGSSSDPTRYDFAGPSAGSELETQCLLQFIRSNVEKEQIKTYIALHSYSQMLMFPYGYTKERVSNYDDLQEFGKKAAAAIKAESGRDYVSGSLYETIYPSSGGSMDWAHAEAGIPIAYTFELRGPPDSQDLFILPAVEIQPTASEAFTAIRTIVEAAAEKGYYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ETN7</accession>
    <name>A0AAU9ETN7_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Indole-3-acetaldehyde oxidase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_08883</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </cofactor>
        <text evidence="18">Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="18">Binds 2 [2Fe-2S] clusters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the xanthine dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9ETN7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.365.10:FF:000009">
        <property type="entry name" value="Aldehyde oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000013">
        <property type="entry name" value="Aldehyde oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.50:FF:000003">
        <property type="entry name" value="Aldehyde oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.50:FF:000003">
        <property type="entry name" value="Aldehyde oxidase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.120:FF:000007">
        <property type="entry name" value="indole-3-acetaldehyde oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.365.10:FF:000019">
        <property type="entry name" value="indole-3-acetaldehyde oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000012">
        <property type="entry name" value="Xanthine dehydrogenase/oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.120">
        <property type="entry name" value="[2Fe-2S]-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.50">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.365.10">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002888">
        <property type="entry name" value="2Fe-2S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036884">
        <property type="entry name" value="2Fe-2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046867">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036683">
        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908:SF132">
        <property type="entry name" value="ALDEHYDE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03450">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00941">
        <property type="entry name" value="FAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01799">
        <property type="entry name" value="Fer2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="MoCoBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20256">
        <property type="entry name" value="MoCoBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000127">
        <property type="entry name" value="Xanthine_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01092">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55447">
        <property type="entry name" value="CO dehydrogenase flavoprotein C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47741">
        <property type="entry name" value="CO dehydrogenase ISP C-domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 18" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="9 17" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11 18" id="KW-0408">Iron</keyword>
    <keyword evidence="12 18" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 18" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="19">
        <location>
            <begin position="3"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="20">
        <location>
            <begin position="207"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="1214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="746"/>
        </location>
        <ligand>
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </ligand>
        <ligandPart>
            <name>Mo</name>
            <dbReference type="ChEBI" id="CHEBI:28685"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="890"/>
        </location>
        <ligand>
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </ligand>
        <ligandPart>
            <name>Mo</name>
            <dbReference type="ChEBI" id="CHEBI:28685"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="1042"/>
        </location>
        <ligand>
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </ligand>
        <ligandPart>
            <name>Mo</name>
            <dbReference type="ChEBI" id="CHEBI:28685"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000127-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000127-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000127-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="BFF90341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1270" mass="138953" checksum="ED5C9CC46672B32C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9EUK5</accession>
    <name>A0AAU9EUK5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transcription factor capicua</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_08180</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="641"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1072"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1396"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1445"/>
            <end position="1519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1536"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1581"/>
            <end position="1609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="303"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="470"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="518"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1085"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1101"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1464"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1498"/>
            <end position="1519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1548"/>
            <end position="1557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF91130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1629" mass="179129" checksum="C872C9C705FD2CA1" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F5N1</accession>
    <name>A0AAU9F5N1_DROMD</name>
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        <recommendedName>
            <fullName evidence="2 7">Peptide-methionine (R)-S-oxide reductase</fullName>
            <ecNumber evidence="2 7">1.8.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_08777</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="2 7"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F5N1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.20:FF:000004">
        <property type="entry name" value="Peptide-methionine (R)-S-oxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00357">
        <property type="entry name" value="peptide-methionine (R)-S-oxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173">
        <property type="entry name" value="METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173:SF52">
        <property type="entry name" value="METHIONINE-R-SULFOXIDE REDUCTASE B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="9">
        <location>
            <begin position="72"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012499"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF90211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="219" mass="24081" checksum="9DD708E580CBECDF" modified="2024-11-27" version="1">MFSLSRHALRHSRNFALIAAAKWRHPNPLGVVAAAATVVPIARCFADSDEGKTNPSKGNNMENKSEKVTVNKEELRKRLTPLQYQVTQEAGTERPYTGCYNKHYEKGVYQCIVCHQDLFSSDTKYDSGCGWPAFNDVLDKGKVTLHRDASIPERIRTEVRCARCNAHMGHVFEDGPKPTRKRYCINSASIEFVTADPTATGAGGGAATTVATPTPIAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F5N9</accession>
    <name>A0AAU9F5N9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">28S ribosomal protein S24 mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_09505</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F5N9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026146">
        <property type="entry name" value="Ribosomal_uS3m"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21244">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21244:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14955">
        <property type="entry name" value="MRP-S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF91160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="164" mass="19095" checksum="47B9DEF54CF47A9B" modified="2024-11-27" version="1">MNFLKKLAPMALAESQICKSDLHTSAVCCRVQSGRYRITTKRNRPLTYEMANPPHFIAHRKSWNSWNTSTLLDSQRPAQTAVEDLFIRKFITGTWHALTCSEIIIKRQHNTIRIASIIRQAISPRKMYFLIGYTEELLSYWMQCPVTLELQTVADKKDVVFKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F601</accession>
    <name>A0AAU9F601_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Dopamine receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_09057</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="entry name" value="OCTOPAMINE RECEPTOR BETA-2R"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 11" id="KW-0297">G-protein coupled receptor</keyword>
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    <keyword evidence="9 11 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
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    <feature type="chain" id="PRO_5043560717" evidence="13">
        <location>
            <begin position="21"/>
            <end position="495"/>
        </location>
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        <location>
            <begin position="130"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="244"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="295"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="414"/>
            <end position="433"/>
        </location>
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        <location>
            <begin position="145"/>
            <end position="430"/>
        </location>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="495" mass="54200" checksum="E152EA9B3E47C5BE" modified="2024-11-27" version="1">MTNAMSALAALALTAAGAAAVAASAASGRSNHSSPFLTNSNSSRATTTAGSISSSIGMAISNSSSISGSGISRNGTLLEADHLPLQLTTAKVDMDFEIDIQLLTNGYDGTTLTSFYNESSWTNASEMDTIVGIFLSVLIFLSVAGNILVCLAIYTERSLRRIGNLFLASLAIADLFVASLVMTFAGVNDLLGYWIFGAQFCDTWVAFDVMCSTASILNLCAISMDRYIHIKDPLRYGRWVTRRVAVITIAAIWLLAAFVSFVPISLGIHRPDQPMIFEDNGKKYPTCALDLTPTYAVVSSCISFYLPCVVMIGIYCRLYCYAQKHVKSIKAVTRPGEVAEKQRYKSIRRPKMTPKKFKVRNLHHSSPYHVSDHKAAVTVGVIMGVFLICWVPFFCVNITAAFCKTCIGGQTFKILTWLGYSNSAFNPIIYSIFNKEFRDAFKRILTMRNPWCCAQDVGNIHPRNSDRFITDYAAKNVVVMNSGRSSAELEQVSAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F608</accession>
    <name>A0AAU9F608_DROMD</name>
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        <submittedName>
            <fullName evidence="7">Zinc transporter ZIP1</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="ORF">DMAD_08129</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
    </feature>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="366"/>
        </location>
    </feature>
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            <begin position="124"/>
            <end position="188"/>
        </location>
    </feature>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF89348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="370" mass="40222" checksum="DA14DCC3A483B0AA" modified="2024-11-27" version="1">MSLATGTMDVVNTPNSSMDATNRGEWEKVLAMLVLGLGSFLSGMLPAIISERNRRRFPLTTSLLLCFGAGILLATALVHILPEVREQMNSKFAEVAMCSGFFIIYFIDEFIHYFFGEAIQHTHTHGHEHEREPQPSSHAQVDPHRDHNGYGATGERAPLLSAQSSTSHSHSHHSENHDHNQGSSSACDDASVEDANARICHTSHTEPCAQSMTGTLGLFVALSLHSAIEGLAIGVQNSATKVLFLLGAVACHKFVMGFCLGLEFRSNPQTTLRSQFVGILVFALGAVCGIGLGMLIVDVPAEWSSKTLPIVQALAGGTLFYVTVCEVIPREKARWHSHPTRRWAGFAQFATVVAGFATMCIINFYLSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6F5</accession>
    <name>A0AAU9F6F5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">BK channel</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_10072</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F6F5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000005">
        <property type="entry name" value="calcium-activated potassium channel subunit alpha-1 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000015">
        <property type="entry name" value="Calcium-activated potassium channel subunit alpha-1 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01449">
        <property type="entry name" value="BKCHANNELA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0630">Potassium</keyword>
    <keyword evidence="7" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="19">
        <location>
            <begin position="339"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="19">
        <location>
            <begin position="835"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="640"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="767"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1175"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="767"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF91895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1205" mass="134005" checksum="83854E580942610D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6F8</accession>
    <name>A0AAU9F6F8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GILT-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_08460</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GILT family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F6F8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016671">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004911">
        <property type="entry name" value="Interferon-induced_GILT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234">
        <property type="entry name" value="GAMMA-INTERFERON INDUCIBLE LYSOSOMAL THIOL REDUCTASE GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234:SF68">
        <property type="entry name" value="GH19763P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03227">
        <property type="entry name" value="GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFF89783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="221" mass="24896" checksum="16707D86A35BD1AB" modified="2024-11-27" version="1">MGSVHVNIILTVLLIFLVWQGIKYHQSLLRKICQHSHFQMKQLHITLLYESLCPDSRNFMHQLGPVHEELQQYMDIQLVPFGKSRSEQSGAIFHCQHGPAECEGNRVQSCVISSTANQSAQVKFVVCQMFALDSSRADQCASEAGLRTDVDHCADSESGTKLQLQAELVTKLYQPSFIPTIVYNGVFNQQLQDHSLRDFRGTVCHLLRQQNLLPSGSPVCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6J7</accession>
    <name>A0AAU9F6J7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="15">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_08549</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="15"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000188">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000225">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF190">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="245"/>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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</entry>
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        <name type="scientific">Drosophila madeirensis</name>
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                <person name="Yamamoto D."/>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Endopterygota</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">RF1</strain>
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        <location>
            <begin position="1"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF91156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
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        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="STKc_CK1_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Casein kinase I gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Casein_kinase-1_gamma_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12605">
        <property type="entry name" value="CK1gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="62"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="384"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="428"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAU9F6M1_DROMD</name>
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        <submittedName>
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        </submittedName>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">RF1</strain>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="468"/>
            <end position="515"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F6M8</accession>
    <name>A0AAU9F6M8_DROMD</name>
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        <recommendedName>
            <fullName evidence="13">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="13">2.3.2.26</ecNumber>
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        <name evidence="18" type="ORF">DMAD_08621</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 20">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
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    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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</entry>
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        <name evidence="13" type="ORF">DMAD_12123</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
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            <taxon>Endopterygota</taxon>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13 14">RF1</strain>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF94460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1756" mass="191918" checksum="426B9BFD173EE1FE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FFQ0</accession>
    <name>A0AAU9FFQ0_DROMD</name>
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        <recommendedName>
            <fullName evidence="9">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
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        <name evidence="13" type="ORF">DMAD_12123</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="9">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAHEXCHNGR"/>
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    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <end position="107"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <end position="274"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <end position="313"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="325"/>
            <end position="344"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <end position="414"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="479"/>
            <end position="500"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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    <feature type="transmembrane region" description="Helical" evidence="11">
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    <feature type="transmembrane region" description="Helical" evidence="11">
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        </location>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003722"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Pfam" id="PF00999"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1238" mass="135851" checksum="640567BC84D53493" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FFQ4</accession>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9 10">RF1</strain>
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    </reference>
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    <feature type="domain" description="SH3" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
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        </location>
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        <location>
            <begin position="966"/>
            <end position="1025"/>
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        <location>
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        <location>
            <begin position="100"/>
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        </location>
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            <begin position="163"/>
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        <location>
            <begin position="294"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF94509.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FFQ5</accession>
    <name>A0AAU9FFQ5_DROMD</name>
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        <recommendedName>
            <fullName evidence="3">GMP synthase (glutamine-hydrolyzing)</fullName>
            <ecNumber evidence="3">6.3.5.2</ecNumber>
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            <fullName evidence="10">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
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        <name evidence="13" type="ORF">DMAD_12108</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
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        <text evidence="1">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
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        <text evidence="2">Homodimer.</text>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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        <location>
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        <ligand>
            <name>ATP</name>
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        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF94492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="683" mass="76591" checksum="40CC3C0A8CB0514C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FG12</accession>
    <name>A0AAU9FG12_DROMD</name>
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        <submittedName>
            <fullName evidence="7">Dedicator of cytokinesis protein 11</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="ORF">DMAD_12234</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="dedicator of cytokinesis protein 9 isoform X1"/>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2_Dock-D"/>
    </dbReference>
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        <property type="entry name" value="C2_DOCK-type_domain"/>
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        <property type="entry name" value="DOCK_C_lobe_A"/>
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        <property type="entry name" value="DOCK_C_lobe_C"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317:SF26">
        <property type="entry name" value="ZIZIMIN, ISOFORM K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11878">
        <property type="entry name" value="DOCK_C-D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <source>
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        </source>
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    <sequence length="2265" mass="256714" checksum="0B6CDE31339D534D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FG20</accession>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        </source>
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            <dbReference type="EMBL" id="BFF94751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="224" mass="24909" checksum="B312C94F6C198A44" modified="2024-11-27" version="1">MASALETLQGLLQTLGELKELPLNRESLESLIYWRDVKKSGIVFGAGLITLVAISSFSVISVFAYLSLLALFGTIAFRIYKSVTQAVQKTSDGHPFKEYLELDLTLSQEKVQNIAGVAVAHINGFTAELRRLFLVEDLIDSIKFGVILWVFTYIGAWFNGMTLVILAFVSLFTLPKVYENNKQSIDTYLDLVRSKLTEITDKIRVAIPIGNKKPVAAAETDKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FG22</accession>
    <name>A0AAU9FG22_DROMD</name>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="7" type="ORF">DMAD_12234</name>
    </gene>
    <organism evidence="7 8">
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        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
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        </lineage>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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        <text evidence="2">Belongs to the DOCK family.</text>
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    <dbReference type="EMBL" id="AP029264">
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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                <person name="Tomihara K."/>
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                <person name="Yamamoto D."/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FGG9</accession>
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        <submittedName>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
        </source>
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        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4 5">RF1</strain>
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        <property type="protein sequence ID" value="BFF94771.1"/>
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        <source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50913"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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            <strain evidence="17 18">RF1</strain>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL HOMOLOG"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="THU_Piezo1"/>
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    </dbReference>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1450"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1515"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1643"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1660"/>
            <end position="1669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1670"/>
            <end position="1682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="2405"/>
            <end position="2424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF15917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF24871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF24874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF94775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2641" mass="298652" checksum="7F84AA93C58A308F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FGL9</accession>
    <name>A0AAU9FGL9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Synaptotagmin 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_12367</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Synaptic vesicle membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FGL9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031045">
        <property type="term" value="C:dense core granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007626">
        <property type="term" value="P:locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08385">
        <property type="entry name" value="C2A_Synaptotagmin-1-5-6-9-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08402">
        <property type="entry name" value="C2B_Synaptotagmin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000007">
        <property type="entry name" value="Synaptotagmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000016">
        <property type="entry name" value="Synaptotagmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF227">
        <property type="entry name" value="SYNAPTOTAGMIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="190"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="321"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="53"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="169"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF94840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="470" mass="52840" checksum="16F9E107DFA4A1A6" modified="2024-11-27" version="1">MPPNAKSEADAKPDEEPAPESATQLESVDQKLEETHHSRFRELDKQEQESAAEEAAAAESQRIAQVETTTRSATTEAQESTTTSLPVIKKIEHAGEVVTEAIAERTGLPTWGVVAIIILVFLVVFGIIFFCVRRFLKKRRTKDGKGKKGVDMKSVQLLGSAYKEKVQPDMEELTENAEEGDEEDKQSEQKLGRLNFKLEYDFNSNSLAVTVIQAEELPALDMGGTSDPYVKVYLLPDKKKKFETKVHRKTLSPVFNETFTFKSLPYADAMNKTLVFAIFDFDRFSKHDQIGEVKVPLCTIDLAQTIEEWRDLVSVEGEGGQLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIAIMQNGKRLKKKKTSIKKCTLNPYYNESFSFEVPFEQIQKICLVVTVVDYDRIGTSEPIGRCILGCMGTGTELRHWSDMLASPRRPIAQWHTLKDPEETDEILKNMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FGM2</accession>
    <name>A0AAU9FGM2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Piezo-type mechanosensitive ion channel component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_12317</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PIEZO (TC 1.A.75) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027272">
        <property type="entry name" value="Piezo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031334">
        <property type="entry name" value="Piezo_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056770">
        <property type="entry name" value="Piezo_THU9_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056769">
        <property type="entry name" value="Piezo_TM1-24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031805">
        <property type="entry name" value="Piezo_TM25-28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056768">
        <property type="entry name" value="THU_Piezo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47049">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47049:SF2">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12166">
        <property type="entry name" value="Piezo_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24874">
        <property type="entry name" value="Piezo_THU9_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24871">
        <property type="entry name" value="Piezo_TM1-24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15917">
        <property type="entry name" value="Piezo_TM25-28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23188">
        <property type="entry name" value="THU_Piezo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="59"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="202"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="17">Piezo-type mechanosensitive ion channel component</fullName>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <feature type="domain" description="Piezo THU9 and anchor" evidence="16">
        <location>
            <begin position="1942"/>
            <end position="2181"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo non-specific cation channel cap" evidence="12">
        <location>
            <begin position="2219"/>
            <end position="2538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="729"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1428"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1616"/>
            <end position="1669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2329"/>
            <end position="2353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2544"/>
            <end position="2570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1432"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1497"/>
            <end position="1511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1625"/>
            <end position="1641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1642"/>
            <end position="1651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1652"/>
            <end position="1664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="2334"/>
            <end position="2353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF15917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF24871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF24874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFF94782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2570" mass="290946" checksum="8244AA88E2980C44" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FGM7</accession>
    <name>A0AAU9FGM7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphorylase b kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_12319</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Glycan biosynthesis; glycogen metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Lipid-anchor</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the phosphorylase b kinase regulatory chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FGM7"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
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        <property type="term" value="C:phosphorylase kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045583">
        <property type="entry name" value="KPBA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008734">
        <property type="entry name" value="PHK_A/B_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749:SF8">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19292">
        <property type="entry name" value="KPBB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5 7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 7" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="6 7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH15-like" evidence="9">
        <location>
            <begin position="54"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphorylase b kinase regulatory subunit alpha/beta C-terminal" evidence="10">
        <location>
            <begin position="909"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="S-farnesyl cysteine" evidence="6">
        <location>
            <position position="1094"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR608734-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF19292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF94786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1097" mass="125031" checksum="9F64CAE36957BEC8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FH00</accession>
    <name>A0AAU9FH00_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pseudouridylate synthase RPUSD2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">RNA pseudouridylate synthase domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_12479</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Pseudouridine synthase that catalyzes pseudouridylation of mRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FH00"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02557">
        <property type="entry name" value="PseudoU_synth_ScRIB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000010">
        <property type="entry name" value="RNA pseudouridine synthase domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="11">
        <location>
            <begin position="202"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="39"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00057241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF94978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="513" mass="58783" checksum="2F27418847963B6C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FH02</accession>
    <name>A0AAU9FH02_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Lipase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_12419</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FH02"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000057">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025483">
        <property type="entry name" value="Lipase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000862">
        <property type="entry name" value="Steryl_ester_lip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043437456" evidence="9">
        <location>
            <begin position="23"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="10">
        <location>
            <begin position="77"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="399"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="429"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000862-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFF94906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="456" mass="51682" checksum="DC2CF7677ECD11D2" modified="2024-11-27" version="1">MPHAKWILCLLGVTLQWLWVDGGYLEDNFPASVIEDAHLSTLQLLEKYKHPAEAHHVTTEDNYILTLHRIARPGAQPVLLVHGLEDTSSTWIVMGPQSGLGYFLYGHGYDVWMGNVRGNRYSRGHQQLNANTDRAYWSFSWHEIGMYDLPAMIDGVLAKTGYEKLSYFGHSQGTTSFFVLASSRPEYNAKIHLMSALAPVAFMAHVKAPLLGIARVGMNMLGESLELLPHSFVYLNQCMQSAGMLKTCLRFYWQIVGKNREEFNMTMFPVVLGHIPGGCNVKQPVHFLQLRSSDRFCQYDYEAKENQKIYGRSSPPDYRLERITAPIALYYGSNDYLSAVEDVQRLAKLLPNVVENHLYKKWNHMDMIWAISARHSIQPKMLEAMRYWESAAGAREHAEATTSCVVEEETAEPVTEAATESPDDEKEVEVESGKEQEAARDENQQQNADVESPSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FH03</accession>
    <name>A0AAU9FH03_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Wiskott-Aldrich syndrome protein family member</fullName>
            <shortName evidence="7">WASP family protein member</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_12474</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Binds actin and the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
            <location evidence="1 7">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SCAR/WAVE family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FH03"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031209">
        <property type="term" value="C:SCAR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000601">
        <property type="term" value="P:positive regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000012">
        <property type="entry name" value="Wiskott-Aldrich syndrome protein family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028288">
        <property type="entry name" value="SCAR/WAVE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12902">
        <property type="entry name" value="WASP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12902:SF1">
        <property type="entry name" value="WISKOTT-ALDRICH SYNDROME PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00246">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="9">
        <location>
            <begin position="590"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="181"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="370"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="498"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="636"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="414"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="530"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="637"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF94965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="659" mass="72027" checksum="702E20CF8D543A9E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWP1</accession>
    <name>A0AAU9FWP1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dynamin</fullName>
            <ecNumber evidence="2">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_00115</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="2"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FWP1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
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    </dbReference>
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    <keyword evidence="8 13" id="KW-0342">GTP-binding</keyword>
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            <begin position="513"/>
            <end position="621"/>
        </location>
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        <location>
            <begin position="654"/>
            <end position="745"/>
        </location>
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        <location>
            <begin position="623"/>
            <end position="652"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="744"/>
            <end position="833"/>
        </location>
    </feature>
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        <location>
            <begin position="630"/>
            <end position="645"/>
        </location>
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            <begin position="767"/>
            <end position="778"/>
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            <dbReference type="ARBA" id="ARBA00067339"/>
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        </source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG00018.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="833" mass="93392" checksum="125B8270592A1D5E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWP2</accession>
    <name>A0AAU9FWP2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-rich repeat flightless-interacting protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_00132</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LRRFIP family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome A"/>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LEUCINE RICH REPEAT IN FLII INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
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        <location>
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            <end position="69"/>
        </location>
    </feature>
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        <location>
            <begin position="110"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="250"/>
            <end position="387"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00008275"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG00045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="395" mass="45023" checksum="7AD888A91CD1347C" modified="2024-11-27" version="1">MESTSVTGRRRNNTRVNAEDNALDQITKEAEARLAARRQARAEAREIRMRELERQQKEQEQNADRVFDMHNSSASFGSDSISTHPARLVFGGLINITRNTASRRSSEDSLEDEGRSLRDFKHELNDVEERFRKAMITNAQLDNDRASQSYEVKLLKDKCETLQEAFAQLQREFKEKSRDCNAIKRNLDRTTVELKLVQGQLCERDSLIAEQGLVIVAIENADGSDAKRALVSVENANVLASVQGSLDVRIQKFSDEKQQLLSEVQKLQKIIKEYKSEGIVGTGSPGPFGKDNDYEAQREANKIISDYKYKLQKAEQEMANLQSSLARSETQVIRYKSTAEAAEKAEAELKVERRKLQREHREVLEKLEETETANNHLLKRLDKLKNAKSAILKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWP3</accession>
    <name>A0AAU9FWP3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">5'-3' exoribonuclease 1</fullName>
            <ecNumber evidence="12">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_00022</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the 5'-3' exonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="12"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFF99888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000004">
        <property type="entry name" value="5'-3' exoribonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000005">
        <property type="entry name" value="5'-3' exoribonuclease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016494">
        <property type="entry name" value="5_3_exoribonuclease_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041385">
        <property type="entry name" value="SH3_12"/>
    </dbReference>
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        <property type="entry name" value="XRN1_D1"/>
    </dbReference>
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        <property type="entry name" value="XRN1_D1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041106">
        <property type="entry name" value="XRN1_D2_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040587">
        <property type="entry name" value="XRN1_DBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047008">
        <property type="entry name" value="XRN1_SH3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF7">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18129">
        <property type="entry name" value="SH3_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18332">
        <property type="entry name" value="XRN1_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18334">
        <property type="entry name" value="XRN1_D2_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18245">
        <property type="entry name" value="XRN1_DBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006743">
        <property type="entry name" value="Exonuclease_Xnr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="15">
        <location>
            <begin position="269"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' exoribonuclease 1 D1" evidence="18">
        <location>
            <begin position="638"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease Xrn1 D2/D3" evidence="19">
        <location>
            <begin position="808"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' exoribonuclease 1 SH3-like" evidence="16">
        <location>
            <begin position="1060"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="domain" description="5-3 exonuclease XRN1 DCP1-binding motif" evidence="17">
        <location>
            <begin position="1341"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1152"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1245"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1484"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1178"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1245"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1487"/>
            <end position="1499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF18129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF18245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF18332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF18334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFF99888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1574" mass="181282" checksum="4EC9C84E5E27A6A9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWP4</accession>
    <name>A0AAU9FWP4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Poly(U)-specific endoribonuclease homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_00092</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ENDOU family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFF99985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FWP4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21159">
        <property type="entry name" value="XendoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039787">
        <property type="entry name" value="ENDOU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037227">
        <property type="entry name" value="EndoU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018998">
        <property type="entry name" value="EndoU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439:SF42">
        <property type="entry name" value="ENDORIBONUCLEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439">
        <property type="entry name" value="PLACENTAL PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09412">
        <property type="entry name" value="XendoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142877">
        <property type="entry name" value="EndoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51959">
        <property type="entry name" value="ENDOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043096968" evidence="11">
        <location>
            <begin position="17"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="EndoU" evidence="13">
        <location>
            <begin position="355"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="58"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="166"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="204"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="287"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFF99985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="618" mass="66494" checksum="E77D8F24E800567B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWP8</accession>
    <name>A0AAU9FWP8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="20">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_07633</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Has a role in the Wnt signaling pathway controlling the asymmetry of cell divisions during embryogenesis. Operates in the AB and EMS cell lineages influencing cell specification. Required for body wall muscle development, endoderm development, pop-1 asymmetry and T-cell division asymmetry. Component of the beta-catenin-lit-1 complex which promotes the phosphorylation, down-regulation and subcellular relocation of pop-1. Regulates plp-1 nuclear localization in embryos. Plays a role in male tail tip morphogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="20">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="20"/>
    <dbReference type="EMBL" id="AP029265">
        <property type="protein sequence ID" value="BFF99831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FWP8"/>
    <dbReference type="SMR" id="A0AAU9FWP8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome J"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07853">
        <property type="entry name" value="STKc_NLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000162">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000961">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 18 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 20 22" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 20" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 18 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="40"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="BFF99831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="414" mass="46443" checksum="A5543C662AB61155" modified="2024-11-27" version="1">MSVSMSLVQGGAAGGAPQGASAILAAAAPYYQPPAVPQDVQPDRPIGYGAFGVVWAVTDPRDGRRVALKKLPNVFQSLVSSKRVFRELKMLCFFKHENVLSALDILQPPHLDFFQEIYVITELLQSDLHKIIVSPQHLSADHIKVFLYQILRGLKYLHSARILHRDIKPGNLLVNSNCVLKICDFGLARVEEPDQAKHMTQEVVTQYYRAPEILMGARHYSSAVDVWSVGCIFGELLGRRILFQAQNPVQQLELITELLGTPTMEDMRHACEGARTHMLRRAPKPPSFSVLYTLSSHATHEAVHLLCQMLVFDPDKRISVTDALAHPYLDEGRLRYHSCMCKCCFTTSAGMRQYTADFEPSAGQPFDDLWERKLTSVQQVKEEMHKFIAEQLQTGRVPLCINPQSAAFKSFASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWQ0</accession>
    <name>A0AAU9FWQ0_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">RING-box protein 1A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_00254</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RING-box family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FWQ0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031462">
        <property type="term" value="C:Cul2-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16485">
        <property type="entry name" value="mRING-H2-C3H2C2D_RBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000010">
        <property type="entry name" value="E3 ubiquitin-protein ligase RBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="57"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFG00214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="112" mass="13139" checksum="19A967AD9A096309" modified="2024-11-27" version="1">MDRNMEVDEEEFEVPSSSSKGEKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWDFQKYGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWQ5</accession>
    <name>A0AAU9FWQ5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Stromal interaction molecule homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_00144</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Endomembrane system</location>
            <topology evidence="13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FWQ5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005246">
        <property type="term" value="F:calcium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002115">
        <property type="term" value="P:store-operated calcium entry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09504">
        <property type="entry name" value="SAM_STIM-1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SOAR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Stromal interaction molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000009">
        <property type="entry name" value="Stromal interaction molecule 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.10.238.180:FF:000001">
        <property type="entry name" value="Stromal interaction molecule 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Stromal interaction molecule homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.180">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand_STIM1/2"/>
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        <property type="entry name" value="SAM"/>
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        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
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        <property type="entry name" value="SOAR_STIM1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037608">
        <property type="entry name" value="STIM1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15136">
        <property type="entry name" value="STROMAL INTERACTION MOLECULE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15136:SF5">
        <property type="entry name" value="STROMAL INTERACTION MOLECULE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25578">
        <property type="entry name" value="EF-hand_STIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16533">
        <property type="entry name" value="SOAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SAM" evidence="16">
        <location>
            <begin position="108"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="209"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="432"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG00060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="465" mass="52967" checksum="CE82C700E6A7A1DF" modified="2024-11-27" version="1">MSQAVSNEFNSVGSGSADGACNPEEIDCYNGNVQDRFGMEAIASLHRQLDDDDNGNIDLSESDDFLREELKYDSGYEKRQRAFHFNDDMHISVKELWEAWLRSEVHNWTIEQTTDWLAQSVQLPQYVDLFKLHKVTGASLPRLAVNNLQYVGNVLGIKDPIHKQKISLKAMDVVLFGPPRETGTRWKDYILVTLLLSAIIGCWYAYQQNKNAKRHLRRMAQDMEGLQKAEQSLQEMQKELERARMEQENVATEKMDLERRLKEAPSLSSSNSDLEVLQLKKEVEMLRNELSRAEFELVDNCWAPPPQLQSWLQYTYELESKNHQKKRASAEKQLQSAREACEKLRKKRSSLVGAFVSTHGKSIDDVDRSIVEARNALGEVTNELQERLHRWKQIETCLGINIVNNNGLSCLENVLYGRNGGFQSSIGGSGSKGSRTRITSSTDDLDDESVQGKLNFENFSLLATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWR6</accession>
    <name>A0AAU9FWR6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Membrane-associated progesterone receptor component 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_00109</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FWR6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000003">
        <property type="entry name" value="membrane-associated progesterone receptor component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF106">
        <property type="entry name" value="IP06960P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="3">
        <location>
            <begin position="5"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG00006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="188" mass="20898" checksum="D8C8A6941D907017" modified="2024-11-27" version="1">MRRDFTVKELRQYDGNQLDGRVLVAVNGNVYDVSKGKRFYGPGGPYSTFAGRDASRNLATFSVVSNDKDEFDDLSDLSAVEMDSVREWEMQFKEKYELVGKLLRTGEEPANYEDDDDDENTVNSEENVVAPPANVLSEQSDGLVKRKTDNVSSETEKDVKKNIADSNILLAKPSTESGPKGELPSKDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FX16</accession>
    <name>A0AAU9FX16_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Transmembrane protein 132E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_00221</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM132 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031437">
        <property type="entry name" value="Ig_TMEM132_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055423">
        <property type="entry name" value="Ig_TMEM132_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055424">
        <property type="entry name" value="Ig_TMEM132_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026307">
        <property type="entry name" value="TMEM132"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055421">
        <property type="entry name" value="TMEM132_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031436">
        <property type="entry name" value="TMEM132_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13388">
        <property type="entry name" value="DETONATOR, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13388:SF11">
        <property type="entry name" value="DETONATOR, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16070">
        <property type="entry name" value="Ig_TMEM132_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23486">
        <property type="entry name" value="Ig_TMEM132_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23487">
        <property type="entry name" value="Ig_TMEM132_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23039">
        <property type="entry name" value="TMEM132_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15706">
        <property type="entry name" value="TMEM132_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043953173" evidence="9">
        <location>
            <begin position="17"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="932"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 cohesin-like" evidence="12">
        <location>
            <begin position="278"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein family 132 fourth" evidence="11">
        <location>
            <begin position="445"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 fifth" evidence="13">
        <location>
            <begin position="545"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 sixth" evidence="14">
        <location>
            <begin position="701"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 C-terminal" evidence="10">
        <location>
            <begin position="907"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1148"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1276"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1303"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1210"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1148"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1188"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1280"/>
            <end position="1294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF15706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG00165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1373" mass="151017" checksum="86F5C7CAB910632B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FX23</accession>
    <name>A0AAU9FX23_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein strawberry notch</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_00201</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SBNO family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000282">
        <property type="entry name" value="Strawberry notch homolog 1 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057332">
        <property type="entry name" value="SBNO_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026937">
        <property type="entry name" value="SBNO_Helicase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026741">
        <property type="entry name" value="SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039187">
        <property type="entry name" value="SNO_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706:SF30">
        <property type="entry name" value="PROTEIN STRAWBERRY NOTCH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706">
        <property type="entry name" value="STRAWBERRY NOTCH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13872">
        <property type="entry name" value="AAA_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13871">
        <property type="entry name" value="Helicase_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25373">
        <property type="entry name" value="SBNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Strawberry notch AAA" evidence="7">
        <location>
            <begin position="372"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="Strawberry notch helicase C" evidence="6">
        <location>
            <begin position="1086"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="domain" description="SBNO alpha/beta" evidence="8">
        <location>
            <begin position="1401"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="821"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1442"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="876"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="890"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="946"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="970"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1446"/>
            <end position="1465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG00136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1620" mass="175848" checksum="0FB6165F01B983D5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FX38</accession>
    <name>A0AAU9FX38_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SET domain-containing protein SmydA-8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_00087</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFF99978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FX38"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053010">
        <property type="entry name" value="SET_SmydA-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46455">
        <property type="entry name" value="SET AND MYND DOMAIN CONTAINING, ARTHROPOD-SPECIFIC, MEMBER 4, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46455:SF5">
        <property type="entry name" value="SET AND MYND DOMAIN CONTAINING, ARTHROPOD-SPECIFIC, MEMBER 4, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="48"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF99978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="537" mass="60665" checksum="5C6FC2759F2B6621" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FX39</accession>
    <name>A0AAU9FX39_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Gamma-aminobutyric acid receptor subunit alpha-6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_00117</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FX39"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099095">
        <property type="term" value="F:ligand-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19049">
        <property type="entry name" value="LGIC_TM_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000065">
        <property type="entry name" value="GABA-gated ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <dbReference type="Pfam" id="PF02932"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    <sequence length="382" mass="44191" checksum="E7CEC1C7704F3A21" modified="2024-11-27" version="1">MRLTIKATCPMELKNFPMDRQSCPLIIGSYGYTNQQLVYEWKNQDDAVSFVPGMTLNQFDLIGMMHRNFTSRRREGDFSVLHVAFNLKRHTGYFLIQVYVPCILIVVLSWVSFWIHREATSDRVGLCVTAVLTLSTISLDSRTDLPKVKYATALDWFLLMSFLYCIATLLEFAGVHYFTKLGSGEIPRLEEHLENIRKNNMPPAHVIEANEEISDEDLEDADSDDVSEQMNSEHDICICSEHDNIRFESAESLPIALDDTDFRKRNALTCPIFNHPTYIFPKTSSRATTMERTTQTEPPVVSRMRQMWLCLKSDQKFRKQRERDAAAEKTEQGRNVSYVNSVSLIDRIARIAFPMSFALFNLLYWLAYGMYKKEFSWSTIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXI7</accession>
    <name>A0AAU9FXI7_DROMD</name>
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        <submittedName>
            <fullName evidence="9">Serine-rich adhesin for platelets</fullName>
        </submittedName>
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        <name evidence="9" type="ORF">DMAD_00198</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9 10">RF1</strain>
        </source>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG00129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1271" mass="133498" checksum="4EAD15F3CD849319" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXM1</accession>
    <name>A0AAU9FXM1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="5 13">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_00512</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Cytosolic glucose-6-phosphate dehydrogenase that catalyzes the first and rate-limiting step of the oxidative branch within the pentose phosphate pathway/shunt, an alternative route to glycolysis for the dissimilation of carbohydrates and a major source of reducing power and metabolic intermediates for fatty acid and nucleic acid biosynthetic processes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
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        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15842"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="5 13"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FXM1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000013">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.40.50.720:FF:000111">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 13" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="9 13" id="KW-0521">NADP</keyword>
    <keyword evidence="10 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="14">
        <location>
            <begin position="17"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="15">
        <location>
            <begin position="194"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013019"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020444"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG00544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="503" mass="58222" checksum="3E4818B5F6E59273" modified="2024-11-27" version="1">MVCEGTHFDGKIPHTFVIFGASGDLAKKKIYPTLWWLYRDDLLPKPTKFCGYARSKLSIAKLKENCKQYMKVQAHEQTKYEEFWALNEYVAGSYDGRTGFELLNQQLELMENRNKANRIFYLALPPSVFEEVTVNIKQICMSVCGWNRVIVEKPFGRDDVSSKALSDHLARLFDEEQLYRIDHYLGKEMVQNLMTIRFGNKILSSTWNRENIASVLITFKEPFGTQGRGGYFDEFGIIRDVMQNHLLQILSLVAMEKPVSCHPDDIRDEKVKVLKSIEALQLDDMVLGQYVGNPQGKTEDERTGYLDDPTVNKDSSTPTYALGVLKINNERWQGVPFILRCGKALNERKAEVRIQYQDVPGDIFEGNTKRNELVIRVQPGEALYFKMMTKSPGITFDIEETELDLTYEHRYKDSYLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPILHRIEKDHIRPLSYQYGSRGPKLADRKCEENNFQYSGSYKWHGSKGTSGTNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXM9</accession>
    <name>A0AAU9FXM9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="3">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_00790</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="3"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FXM9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004706">
        <property type="term" value="F:JUN kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11876">
        <property type="entry name" value="SH3_MLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14061">
        <property type="entry name" value="STKc_MLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000076">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF293">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 13" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="32"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="118"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="542"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="656"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="744"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="969"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1139"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1232"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="402"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="553"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="586"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="656"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="744"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="767"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="991"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1001"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1018"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="1144"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1249"/>
            <end position="1260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFG00893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1260" mass="138370" checksum="769A35C85EC00A09" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXN1</accession>
    <name>A0AAU9FXN1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphorylase b kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_00452</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Glycan biosynthesis; glycogen metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Lipid-anchor</topology>
            <orientation evidence="2 13">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the phosphorylase b kinase regulatory chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000004">
        <property type="entry name" value="Phosphorylase b kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045583">
        <property type="entry name" value="KPBA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008734">
        <property type="entry name" value="PHK_A/B_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749:SF7">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19292">
        <property type="entry name" value="KPBB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="10 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 13" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="11 13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 13" id="KW-0636">Prenylation</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH15-like" evidence="15">
        <location>
            <begin position="8"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphorylase b kinase regulatory subunit alpha/beta C-terminal" evidence="16">
        <location>
            <begin position="1097"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="881"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="916"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="916"/>
            <end position="954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF19292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG00463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1367" mass="151751" checksum="9C2924BC82CE0D3B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FXN3</accession>
    <name>A0AAU9FXN3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Voltage-dependent T-type calcium channel subunit alpha-1G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_00215</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008332">
        <property type="term" value="F:low voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070509">
        <property type="term" value="P:calcium ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000018">
        <property type="entry name" value="Voltage-dependent T-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000120">
        <property type="entry name" value="Voltage-dependent T-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000125">
        <property type="entry name" value="Voltage-dependent T-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000007">
        <property type="entry name" value="Voltage-dependent T-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000008">
        <property type="entry name" value="Voltage-dependent T-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000009">
        <property type="entry name" value="Voltage-dependent T-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000012">
        <property type="entry name" value="Voltage-dependent T-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005445">
        <property type="entry name" value="VDCC_T_a1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF230">
        <property type="entry name" value="CA[2+]-CHANNEL PROTEIN ALPHA[[1]] SUBUNIT T, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01629">
        <property type="entry name" value="TVDCCALPHA1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="589"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="627"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1403"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1495"/>
            <end position="1519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="2251"/>
            <end position="2272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="2348"/>
            <end position="2370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="2451"/>
            <end position="2474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="2531"/>
            <end position="2552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="2564"/>
            <end position="2588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="2664"/>
            <end position="2684"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="2749"/>
            <end position="2775"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="17">
        <location>
            <begin position="323"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="17">
        <location>
            <begin position="1272"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="17">
        <location>
            <begin position="2212"/>
            <end position="2484"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="17">
        <location>
            <begin position="2529"/>
            <end position="2784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="25"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="764"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="814"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="988"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1084"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1142"/>
            <end position="1212"/>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <sequence length="346" mass="37826" checksum="77DF1AE14060C59E" modified="2024-11-27" version="1">MSFGNIGNTSDLECDKNEYPRQRPTGIVTKVAAHKLSSEPKWADKRDDDSDSEVSSPDNFLTKGAFLLTPSYELSMERAALICEKMSFKGCFSLTKTATGILFKFSHPDDYQAVFKKGFHKVTGARFYRKIAIPCRPRKTFTLYVLDVPEDLPVEDVRHAMYKFDSVVEVVRLHIYSSPSSSSSSSNVNANASSAAAAAAAAAASAEKSAEGEQTLRRAAALRSNVLSKIGGSKILGSLSGSVVGDAIDKAERPERRELPPAVIRITLASMDEYNVLLQNGLNFYDATFFPTEANISLKGARIDYKRRMLDGSIPGRVRELLPVFDAAGFCKLPPPTSKLIKPPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FY08</accession>
    <name>A0AAU9FY08_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WW domain-containing adapter protein with coiled-coil homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DMAD_00474</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FY08"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000089">
        <property type="entry name" value="Blast:WW domain-containing adapter protein with coiled-coil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038867">
        <property type="entry name" value="WAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15911">
        <property type="entry name" value="WW DOMAIN-CONTAINING ADAPTER PROTEIN WITH COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15911:SF6">
        <property type="entry name" value="WW DOMAIN-CONTAINING ADAPTER PROTEIN WITH COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="5">
        <location>
            <begin position="241"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="280"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="454"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="127"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="178"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="347"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="454"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="481"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFG00498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="870" mass="92797" checksum="D187794E451F22C5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FY12</accession>
    <name>A0AAU9FY12_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_00464</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FY12"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055121">
        <property type="entry name" value="HTH_69"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47705">
        <property type="entry name" value="AGAP000321-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47705:SF1">
        <property type="entry name" value="PNP_UDP_1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22979">
        <property type="entry name" value="HTH_69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Winged helix-turn-helix" evidence="2">
        <location>
            <begin position="261"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG00481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="673" mass="74815" checksum="4B7D9AA0F2DC075F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FY13</accession>
    <name>A0AAU9FY13_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Disks large 1 tumor suppressor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_00552</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043297">
        <property type="term" value="P:apical junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009792">
        <property type="term" value="P:embryo development ending in birth or egg hatching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097120">
        <property type="term" value="P:receptor localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06723">
        <property type="entry name" value="PDZ1_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06724">
        <property type="entry name" value="PDZ2_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06795">
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="1">Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.</text>
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        </source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0A4</accession>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </subcellularLocation>
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        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    </dbReference>
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        </location>
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    </evidence>
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        </source>
    </evidence>
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        </source>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1192" mass="130023" checksum="55BAD847A21FD8A4" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0A9</accession>
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        <lineage>
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            <taxon>Insecta</taxon>
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        </lineage>
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">RF1</strain>
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        </location>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFG01363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="68" mass="7677" checksum="113B296F4FBC8CFF" modified="2024-11-27" version="1">MPENPQQPEGQVGNEPPLVRAPPPSPRPRQLLFNQKIFSRLFDEAVATSKRRQQQAKAKLADGLNQRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="5">Double-stranded RNA-specific editase Adar</fullName>
        </submittedName>
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    <gene>
        <name evidence="5" type="ORF">DMAD_00828</name>
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    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
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    <reference evidence="5 6" key="1">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Uncharacterized protein, isoform I"/>
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        <property type="entry name" value="A_deamin"/>
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        <property type="entry name" value="dsRBD_dom"/>
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    <dbReference type="PANTHER" id="PTHR10910:SF62">
        <property type="entry name" value="AT07585P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910">
        <property type="entry name" value="EUKARYOTE SPECIFIC DSRNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02137">
        <property type="entry name" value="A_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ADEAMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="dsRNA-binding domain-like"/>
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        <property type="entry name" value="A_DEAMIN_EDITASE"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="55"/>
            <end position="121"/>
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    <feature type="domain" description="DRBM" evidence="3">
        <location>
            <begin position="172"/>
            <end position="229"/>
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    <feature type="domain" description="A to I editase" evidence="4">
        <location>
            <begin position="305"/>
            <end position="629"/>
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    </feature>
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        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50141"/>
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        <source>
            <dbReference type="EMBL" id="BFG00956.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
    <sequence length="633" mass="70252" checksum="F926D90D196B5D63" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0B8</accession>
    <name>A0AAU9G0B8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase</fullName>
            <ecNumber evidence="3 13">2.7.4.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DMAD_01588</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, may regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, and exocytosis. Phosphorylates inositol hexakisphosphate (InsP6) at position 1 to produce PP-InsP5 which is in turn phosphorylated by IP6Ks to produce (PP)2-InsP4. Alternatively, phosphorylates PP-InsP5 at position 1, produced by IP6Ks from InsP6, to produce (PP)2-InsP4.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1D-myo-inositol hexakisphosphate + ATP = 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:37459"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:74946"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:37460"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + ATP + H(+) = 1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:10276"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="ChEBI" id="CHEBI:77983"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:10277"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
            <location evidence="1 13">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the histidine acid phosphatase family. VIP1 subfamily.</text>
    </comment>
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    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033857">
        <property type="term" value="F:5-diphosphoinositol pentakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000828">
        <property type="term" value="F:inositol hexakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037446">
        <property type="entry name" value="His_Pase_VIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040557">
        <property type="entry name" value="VIP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750">
        <property type="entry name" value="DIPHOSPHOINOSITOL PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750:SF9">
        <property type="entry name" value="INOSITOL HEXAKISPHOSPHATE AND DIPHOSPHOINOSITOL-PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18086">
        <property type="entry name" value="PPIP5K2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="VIP1 N-terminal" evidence="15">
        <location>
            <begin position="180"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="44"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="95"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1720"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1779"/>
            <end position="1798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1858"/>
            <end position="1897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1080"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1107"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1174"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1226"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1410"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1443"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1621"/>
            <end position="1634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1720"/>
            <end position="1733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="1735"/>
            <end position="1753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1867"/>
            <end position="1876"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005609"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BFG01952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1897" mass="209889" checksum="4E3DB36C1356ED6F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0B9</accession>
    <name>A0AAU9G0B9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">furin</fullName>
            <ecNumber evidence="16">3.4.21.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DMAD_01284</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Release of mature proteins from their proproteins by cleavage of -Arg-Xaa-Yaa-Arg-|-Zaa- bonds, where Xaa can be any amino acid and Yaa is Arg or Lys. Releases albumin, complement component C3 and von Willebrand factor from their respective precursors.</text>
            <dbReference type="EC" id="3.4.21.75"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.75" evidence="16"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000001">
        <property type="entry name" value="Furin 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000020">
        <property type="entry name" value="Furin-like protease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000055">
        <property type="entry name" value="Furin-like protease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000096">
        <property type="entry name" value="Furin-like protease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000072">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.850:FF:000001">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.850">
        <property type="entry name" value="Peptidase S8, pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032778">
        <property type="entry name" value="GF_recep_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032815">
        <property type="entry name" value="S8_pro-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF23">
        <property type="entry name" value="FURIN-LIKE PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14843">
        <property type="entry name" value="GF_recep_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16470">
        <property type="entry name" value="S8_pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 19 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 19 20" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 19 20" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1459"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="23">
        <location>
            <begin position="678"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="273"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="388"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="280"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="403"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="18 19">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="18 19">
        <location>
            <position position="420"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="18 19">
        <location>
            <position position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00053600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="BFG01559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1644" mass="179593" checksum="627DA6034D33519D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G194</accession>
    <name>A0AAU9G194_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Polyhomeotic-proximal chromatin protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_01757</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG02183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09577">
        <property type="entry name" value="SAM_Ph1_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050548">
        <property type="entry name" value="PcG_chromatin_remod_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012313">
        <property type="entry name" value="Znf_FCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038603">
        <property type="entry name" value="Znf_FCS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247:SF138">
        <property type="entry name" value="POLYHOMEOTIC DISTAL, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51024">
        <property type="entry name" value="ZF_FCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Yamamoto D."/>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG03909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="224" mass="24968" checksum="1DA2E0F9F4E877FB" modified="2024-11-27" version="1">MADEDITLNEDQLLESMEETNGEQETEIVTETEEEGSMQIDPELEAIKARVKEMEEEAEKIKQMQSEVDKQMAGGSTTGLAAVPLSLEEKQEIDTRSVYVGNVDYGASAEELEAHFHGCGTINRVTILCNKADGHPKGFAYIEFGSKEFVETALAMNETLFRGRQIKVMSKRTNRPGLSTTNRFARGSFRGRGARASRACCHSTFRGARRAMGYRGRANYYAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G8R1</accession>
    <name>A0AAU9G8R1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Ribophorin-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_03737</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. STT3A complex assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258, subcomplex 2 contains the STT3A-specific subunits STT3A, DC2/OSTC, and KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2, DAD1, and OST48. The STT3A complex can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Interacts with DDI2. Interacts with TMEM35A/NACHO.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Endoplasmic reticulum membrane</location>
            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the SWP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G8R1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055375">
        <property type="entry name" value="Ribophorin_II_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055374">
        <property type="entry name" value="Ribophorin_II_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056790">
        <property type="entry name" value="Ribophorin_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055373">
        <property type="entry name" value="Ribophorin_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008814">
        <property type="entry name" value="Swp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640">
        <property type="entry name" value="RIBOPHORIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05817">
        <property type="entry name" value="Ribophorin_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23861">
        <property type="entry name" value="Ribophorin_II_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23860">
        <property type="entry name" value="Ribophorin_II_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25147">
        <property type="entry name" value="Ribophorin_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043113488" description="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2" evidence="12">
        <location>
            <begin position="21"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="542"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="575"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="601"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II N-terminal" evidence="13">
        <location>
            <begin position="29"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II second" evidence="15">
        <location>
            <begin position="274"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II third" evidence="14">
        <location>
            <begin position="379"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II C-terminal" evidence="16">
        <location>
            <begin position="528"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF25147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG04877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="636" mass="69662" checksum="A17F65BA09CA7B33" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G8R3</accession>
    <name>A0AAU9G8R3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Probable nuclear hormone receptor HR3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Nuclear receptor subfamily 1 group F member 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_03091</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Putative receptor whose ligand is not yet known.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G8R3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06968">
        <property type="entry name" value="NR_DBD_ROR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000037">
        <property type="entry name" value="Hormone receptor 3, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000003">
        <property type="entry name" value="Nuclear orphan receptor ROR-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044101">
        <property type="entry name" value="NR_DBD_ROR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001728">
        <property type="entry name" value="ThyrH_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45805">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR HR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45805:SF2">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR HR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00546">
        <property type="entry name" value="THYROIDHORMR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 14 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="16">
        <location>
            <begin position="40"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="17">
        <location>
            <begin position="229"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="137"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFG04017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="479" mass="54204" checksum="480E102706DF9229" modified="2024-11-27" version="1">MWSSVTSKLEAHANNLGQSNVQSPAGQNNSSGSIKAQIEIIPCKVCGDKSSGVHYGVITCEGCKGFFRRSQSSVVNYQCPRNKQCVVDRVNRNRCQYCRLQKCLKLGMSRDAVKFGRMSKKQREKVEDEVRFHRAQMRAQSDAAPDSSVYDTQTPSSSDQLHHNSYNSYSGGYSNNEVGYGSPYGYSASVTPQQTMQYDISADYVDSTTYEPRSTIIDPEFISHADGDINDVLIKTLAEAHANTNTKLEAVHDMFRKQQDISRILYYKNLGQEELWLDCAEKLTQMIQNIIEFAKLIPGFMRLSQDDQILLLKTGSFELAIVRMSRLLDLSQNAVLYGDVMLPQEAFYTSDSEEMRLVSRIFQTAKSIAELKLTETELALYQSLVLLWPERNGVRGNTEIQRLFNLSMNAIRQELETNHAPLKGDVTVLDTLLNNIPNFRDISILHMESLSKFKQTHPTVVFPALYKELFSIDSQQDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G910</accession>
    <name>A0AAU9G910_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="15">ATP-PFK</shortName>
            <shortName evidence="15">Phosphofructokinase</shortName>
            <ecNumber evidence="15">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_03389</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 15">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15 16">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="15">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 15 16">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="15"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G910"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000001">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000003">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000043">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase, platelet type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF4">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 15 16" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 15 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="17">
        <location>
            <begin position="188"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="17">
        <location>
            <begin position="579"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="15">
        <location>
            <begin position="1"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="15">
        <location>
            <begin position="579"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="258"/>
            <end position="259"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="288"/>
            <end position="291"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="334"/>
            <end position="336"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="378"/>
            <end position="380"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="468"/>
            <end position="471"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="648"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="705"/>
            <end position="709"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="743"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="750"/>
            <end position="752"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="806"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="832"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="838"/>
            <end position="841"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="913"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFG04410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="956" mass="106013" checksum="A78CD7BF9BEFA3CD" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G913</accession>
    <name>A0AAU9G913_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Pre-mRNA cleavage complex 2 protein Pcf11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Pre-mRNA cleavage complex II protein Pcf11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_03910</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of pre-mRNA cleavage complex II, which promotes transcription termination by RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Associates with the phosphorylated CTD domain of POLR2A /RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16982">
        <property type="entry name" value="CID_Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000015">
        <property type="entry name" value="Pre-mRNA cleavage complex 2 protein Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045154">
        <property type="entry name" value="PCF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054127">
        <property type="entry name" value="Pcf11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047415">
        <property type="entry name" value="Pcf11_CID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048830">
        <property type="entry name" value="PCF11_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921:SF3">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX 2 PROTEIN PCF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21936">
        <property type="entry name" value="Pcf11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20845">
        <property type="entry name" value="Pcf11_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="CID" evidence="16">
        <location>
            <begin position="11"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="260"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="563"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="652"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="729"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1329"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1410"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1494"/>
            <end position="1547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1605"/>
            <end position="1627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1694"/>
            <end position="1717"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="190"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="312"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="452"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="491"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="652"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="735"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1429"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1441"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1523"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1605"/>
            <end position="1621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1694"/>
            <end position="1716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG05093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1868" mass="203383" checksum="08AA9BFF5102EF07" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G925</accession>
    <name>A0AAU9G925_DROMD</name>
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        <submittedName>
            <fullName evidence="10">Protein eiger</fullName>
        </submittedName>
    </protein>
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        <name evidence="10" type="ORF">DMAD_03397</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
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        </subcellularLocation>
    </comment>
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        <text evidence="2">Belongs to the tumor necrosis factor family.</text>
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    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
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            <end position="58"/>
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    <feature type="domain" description="THD" evidence="9">
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        </location>
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            <end position="160"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="7">
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            <end position="205"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    <sequence length="412" mass="46202" checksum="BD0F55277669F7D0" modified="2024-11-27" version="1">MTAETLKPFITPTSAAGSFPAKQSSAAKAQRKTRQIIPIVLSVVGLALVVAILSLTIWQSSRVTHLDKELQSLKRVVSSLQQRLGIQYLDELDDFQKEYENALIEFPNKLDGIPDDDDDDGDSDGDEDIDADADDEVDGFPDFDPDETSSLYDEYDEPDDPTPAPNVEEPDGNTSASSASNDDNVYEDFTNYNDSKKKKIGRKSRSIAEADVRNELQTAQTNQTEQTETKESTPSVQHHHRRKLRPRTSRQQVLVRKAKSTTNSGSRTPAAHFHLNRKVSHHNAPIQLSSHQGDMYIGHATAGSDTAWQEYFSVSNGVLTAHQPGLYYVYAQICYNNTHDQNGFIVFHGHNAFLQCLNTVPTNMPQKVHTCHTSGLIQLREQESIHLRDIHRDRNVLLRDSNNRSYFGIIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G926</accession>
    <name>A0AAU9G926_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">ATP-dependent zinc metalloprotease YME1L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_03538</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G926"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000002">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000175">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF97">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE YME1L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 17" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="342"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG04609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="746" mass="81432" checksum="406622352D9AD36E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G929</accession>
    <name>A0AAU9G929_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Grh/CP2 DB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_03084</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the grh/CP2 family. CP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G929"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09537">
        <property type="entry name" value="SAM_CP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000073">
        <property type="entry name" value="Gemini, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007604">
        <property type="entry name" value="CP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041418">
        <property type="entry name" value="SAM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040167">
        <property type="entry name" value="TF_CP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037:SF21">
        <property type="entry name" value="GEMINI, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037">
        <property type="entry name" value="TRANSCRIPTION FACTOR CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04516">
        <property type="entry name" value="CP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18016">
        <property type="entry name" value="SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51968">
        <property type="entry name" value="GRH_CP2_DB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Grh/CP2 DB" evidence="4">
        <location>
            <begin position="398"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="27"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="89"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="197"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="272"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="350"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="553"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="623"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="154"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="553"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="623"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFG03999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="948" mass="104614" checksum="9969BD1977CD9FFB" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G935</accession>
    <name>A0AAU9G935_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Microtubule-actin cross-linking factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_03839</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="8135"/>
            <end position="8145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="8460"/>
            <end position="8480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="8518"/>
            <end position="8542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="8558"/>
            <end position="8567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="8574"/>
            <end position="8593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="8594"/>
            <end position="8608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="8630"/>
            <end position="8664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG04997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="8673" mass="975317" checksum="AAB5E0BAB1D68489" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G937</accession>
    <name>A0AAU9G937_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-hexosaminidase</fullName>
            <ecNumber evidence="7">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_03067</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="7"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G937"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016231">
        <property type="term" value="F:beta-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030203">
        <property type="term" value="P:glycosaminoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06562">
        <property type="entry name" value="GH20_HexA_HexB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000063">
        <property type="entry name" value="Beta-hexosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="entry name" value="Chitobiase/beta-hexosaminidase domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029019">
        <property type="entry name" value="HEX_eukaryotic_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600">
        <property type="entry name" value="BETA-HEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600:SF3">
        <property type="entry name" value="BETA-HEXOSAMINIDASE FDL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14845">
        <property type="entry name" value="Glycohydro_20b2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001093">
        <property type="entry name" value="B-hxosamndse_ab_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55545">
        <property type="entry name" value="beta-N-acetylhexosaminidase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-hexosaminidase eukaryotic type N-terminal" evidence="11">
        <location>
            <begin position="122"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 20 catalytic" evidence="10">
        <location>
            <begin position="295"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001093-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG03971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="679" mass="77449" checksum="35771BBB9FDA9A43" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G939</accession>
    <name>A0AAU9G939_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">carnitine O-palmitoyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_03120</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(R)-carnitine + hexadecanoyl-CoA = O-hexadecanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:12661"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:17490"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="EC" id="2.3.1.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:12662"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.21" evidence="5"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G939"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004095">
        <property type="term" value="F:carnitine O-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000001">
        <property type="entry name" value="Carnitine O-palmitoyltransferase 1, liver isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000042">
        <property type="entry name" value="Carnitine Palmitoyl Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032476">
        <property type="entry name" value="CPT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF31">
        <property type="entry name" value="CARNITINE O-PALMITOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16484">
        <property type="entry name" value="CPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Carnitine O-palmitoyltransferase N-terminal" evidence="19">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="18">
        <location>
            <begin position="174"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF16484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFG04070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="780" mass="89208" checksum="3DA248DF0CB99CED" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G9M5</accession>
    <name>A0AAU9G9M5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="6">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_03276</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="6"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G9M5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090433">
        <property type="term" value="F:palmitoyl-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17639">
        <property type="entry name" value="LC_FACS_euk1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000023">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000026">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF83">
        <property type="entry name" value="ACYL-COA SYNTHETASE LONG-CHAIN, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="8">
        <location>
            <begin position="108"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG04265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="707" mass="79467" checksum="EC4DA88C05BD896C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G9M9</accession>
    <name>A0AAU9G9M9_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SH3 and multiple ankyrin repeat domains protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_03341</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030160">
        <property type="term" value="F:synaptic receptor adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17091">
        <property type="entry name" value="FERM_F0_SHANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06746">
        <property type="entry name" value="PDZ_SHANK1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000337">
        <property type="entry name" value="AF4/FMR2 family member 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000178">
        <property type="entry name" value="SH3 and multiple ankyrin repeat domains protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000029">
        <property type="entry name" value="SH3 and multiple ankyrin repeat domains protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000018">
        <property type="entry name" value="SH3 and multiple ankyrin repeat domains protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032425">
        <property type="entry name" value="FERM_f0"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051569">
        <property type="entry name" value="SHANK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24135:SF28">
        <property type="entry name" value="LD13733P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24135">
        <property type="entry name" value="SH3 AND MULTIPLE ANKYRIN REPEAT DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16511">
        <property type="entry name" value="FERM_f0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="194"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="261"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="294"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="385"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="574"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="458"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="683"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="753"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="784"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="856"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="983"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1075"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1239"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1466"/>
            <end position="1485"/>
        </location>
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</entry>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <property type="entry name" value="Peptidase_S1A"/>
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        <property type="entry name" value="NOTOPLEURAL, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CHYMOTRYPSIN"/>
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    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRYPSIN_DOM"/>
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    </dbReference>
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        <property type="entry name" value="TRYPSIN_HIS"/>
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        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="62"/>
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    </feature>
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        <location>
            <begin position="840"/>
            <end position="1082"/>
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            <begin position="214"/>
            <end position="225"/>
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        <location>
            <begin position="323"/>
            <end position="378"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="8">
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            <begin position="379"/>
            <end position="391"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="8">
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            <end position="410"/>
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        <source>
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        <source>
            <dbReference type="EMBL" id="BFG05290.1"/>
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    </evidence>
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    <sequence length="1083" mass="115542" checksum="1A393F5B3417CF33" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G9N4</accession>
    <name>A0AAU9G9N4_DROMD</name>
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        <recommendedName>
            <fullName evidence="11">Guanine nucleotide-binding protein subunit alpha</fullName>
        </recommendedName>
    </protein>
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        <name evidence="12" type="ORF">DMAD_03201</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the G-alpha family. G(q) subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="BFG04166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome E"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 10 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
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        <location>
            <position position="47"/>
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        <ligand>
            <name>Mg(2+)</name>
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        <ligand>
            <name>GTP</name>
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        <location>
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        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
    </feature>
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        <location>
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            <end position="203"/>
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        <ligand>
            <name>GTP</name>
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            <name>GTP</name>
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        <ligand>
            <name>GTP</name>
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    </evidence>
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    </evidence>
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            <dbReference type="EMBL" id="BFG04166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="353" mass="41523" checksum="66BDE85072A86E50" modified="2024-11-27" version="1">MECCLSEEAKEQKRINQEIEKQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGYIKLVFQNIFMAMQSMIKAMDMLKISYGVGEHSELADLVMSIDYETVTTFEDPFLNAIKTLWADAGIQECYDRRREYQLTDSAKYYLMDLDRVAQPAYLPTEQDILRVRVPTTGIIEYPFDLEEIRFRMVDVGGQRSERRKWIHCFENVTSIIFLVALSEYDQILFESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAIAAREFILRMFVDLNPDSEKIIYSHFTCATDTENIRFVFAAVKDTILQSNLKEYNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G9N5</accession>
    <name>A0AAU9G9N5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glutathione S-transferase theta-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_03983</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Theta family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G9N5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03183">
        <property type="entry name" value="GST_C_Theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000039">
        <property type="entry name" value="Glutathione S-transferase theta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040077">
        <property type="entry name" value="GST_C_Theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051369">
        <property type="entry name" value="GST_Theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917:SF8">
        <property type="entry name" value="GH16740P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="68"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFG05192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="206" mass="23964" checksum="7F76BE1A76F96BBC" modified="2024-11-27" version="1">MKLGKIPFDDCPVALRKREHLTDEYKKINRFQKVPAIVDGNFQLSESVAIVRYLSDKGQFSEKLYPKSLEARARVDEFLEWQHLGIRVPCGLFFRSVWLYPVNGIAPMPKTEKIQKLVKDVETNLEFVERLWLEKDFLIGDQLTVADIFGASEINQMKLCQYNVSEKQFPKVAKWLARVRESSNPYFDEAHNFVYEKSKQAVSAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G9P3</accession>
    <name>A0AAU9G9P3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Resistance to inhibitors of cholinesterase protein 3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_03690</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ric-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G9P3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034394">
        <property type="term" value="P:protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007271">
        <property type="term" value="P:synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026160">
        <property type="entry name" value="Ric3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032763">
        <property type="entry name" value="RIC3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21723:SF3">
        <property type="entry name" value="PROTEIN RIC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21723">
        <property type="entry name" value="RESISTANCE TO INHIBITORS OF CHOLINESTERASE PROTEIN 3 RIC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15361">
        <property type="entry name" value="RIC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Resistance to inhibitors of cholinesterase protein 3 N-terminal" evidence="9">
        <location>
            <begin position="38"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="281"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="281"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF15361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG04819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="470" mass="51187" checksum="80F8E911DBC74746" modified="2024-11-27" version="1">MPSAAVAPSQPATAKKRPSAVKEEGMTPKKTALIIVTVIGCIAILWPKVFHPMMFGSAPLPKQQNPKEQRSGAPGGCCDVVLDREQFMNASKPDAAVPFGPHLYRKHVNLYTGEISLRQERPAHLHPESIYQAMRERGRAIPATPTVPIVERKSSPSNPPPRIVDGRPGPIPGMRPPMGAGALQQPQQRGSSMGFLMPLYTIGIVVFFGYTIMKIAFKKQVPNAPYGPAPTDPGFRQQVFGQQAHSQVEDLGGTKLGWREHQTIVTAIQGLIDAADEQLNVRDKQRSDTDTETDLNKQNDSEKTKEQLEKENISNGHVNTISDQGAAGKQQQRKRRDLSAERELTVLGMEVTASCEGGGKWTGRPPTPVFRAPSEHSKLEGNLPEPQSIYLEGALAHDSQILVTDAQTKREEVYDAELNGSAEEPAVILSSKMTLSLINLDANQQNGNVGKPGIESPLADDIEIIGHDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAA4</accession>
    <name>A0AAU9GAA4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Focal adhesion kinase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_04157</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAA4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007172">
        <property type="term" value="P:signal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13190">
        <property type="entry name" value="FERM_C_FAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05056">
        <property type="entry name" value="PTKc_FAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000039">
        <property type="entry name" value="Focal adhesion kinase, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000629">
        <property type="entry name" value="Focal adhesion kinase, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049385">
        <property type="entry name" value="FAK1-like_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041784">
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</entry>
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                <person name="Yamamoto D."/>
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</entry>
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                <person name="Yamamoto D."/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1388"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1483"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1568"/>
            <end position="1592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1598"/>
            <end position="1616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFG05530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1624" mass="172332" checksum="91911778F0D335E6" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAC6</accession>
    <name>A0AAU9GAC6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Aquaporin AQPcic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_03588</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAC6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000023">
        <property type="entry name" value="Prip, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF199">
        <property type="entry name" value="MIP17260P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="240"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG04686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="264" mass="28422" checksum="C2BB220504EFADD3" modified="2024-11-27" version="1">MGKFEYSLGLNELKSKELRLWQALIGEFLGTLILNFFACGACTQVEDGTFKALAFGLAIFMAITIVGHLSGGHVNPAVTVGMLVAGRISFLRALFYIVFQCLGAIAGTAAVRTLIDRDYYNGLGHTSLAENITELQGLGIEFFLGLLLVLTVFGACDPHKPDSRYTAPLAIGMAVTLGHLGTIRYTGASMNPARTVGTAFATDNWSAHWVYWAGPVLGGVAAALLYTQVLEAKPAPKTAEAADKYRTHADERELRKLEGTRDYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAC9</accession>
    <name>A0AAU9GAC9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutaminase</fullName>
            <ecNumber evidence="3">3.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">L-glutamine amidohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_03510</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="3"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAC9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:L-glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.210:FF:000002">
        <property type="entry name" value="Blast:Glutaminase kidney isoform, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000069">
        <property type="entry name" value="Glutaminase, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.710.10:FF:000008">
        <property type="entry name" value="Glutaminase, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00313">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015868">
        <property type="entry name" value="Glutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041541">
        <property type="entry name" value="Glutaminase_EF-hand"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03814">
        <property type="entry name" value="Gln_ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544">
        <property type="entry name" value="GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544:SF29">
        <property type="entry name" value="GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17959">
        <property type="entry name" value="EF-hand_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04960">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Glutaminase EF-hand" evidence="11">
        <location>
            <begin position="83"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="540"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="15"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="604"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="623"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00077251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG04572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="669" mass="74452" checksum="77F33A3048E441EE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAD5</accession>
    <name>A0AAU9GAD5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7153 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_03927</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAD5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055577">
        <property type="entry name" value="DUF7153"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22198">
        <property type="entry name" value="FERM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22198:SF1">
        <property type="entry name" value="FERM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23672">
        <property type="entry name" value="DUF7153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7153" evidence="2">
        <location>
            <begin position="177"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="10"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG05114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="364" mass="40204" checksum="132975C67907502D" modified="2024-11-27" version="1">MNLMFRKNFREGASKSGGGGGKLQSKANRTKRSRDMGLVQQPEEIHYRTHLFFSPNRPGYDVGEVNFGGGGHTGVTRAEPISLATLDRDCFIIPVHSVDRFLPAGIPLPALSADGKTASPLSVLEVSDPKLCILVHLMSPLEAIDPVMESPLSHPLLKQRSIASELVTEVQQANTAVGGMLLANMEKNSEFPFISYYLINTLQTDPSNFYASLRVSSLSKFEPKALKYTAAHTLDLYSEVASICRPPLVLPSNEAGSFKKSQTAATGYIITVFKVFEGDDGERFEKNWLYWTGARMLYRYLPRAAGLRRIALHKSTSQKGDKMYLLVCECADLLKDISLAAFLIPAMRARLCGYTGLYRPIQAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAY3</accession>
    <name>A0AAU9GAY3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aspartyl/asparaginyl beta-hydroxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_04039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAY3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062101">
        <property type="term" value="F:peptidyl-aspartic acid 3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039038">
        <property type="entry name" value="ASPH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12366:SF29">
        <property type="entry name" value="ASPARTYL BETA-HYDROXYLASE, ISOFORM L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12366">
        <property type="entry name" value="ASPARTYL/ASPARAGINYL BETA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="244"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="606"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG05269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="645" mass="73011" checksum="805CB5CDE2F3CF4C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GBA6</accession>
    <name>A0AAU9GBA6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Solute carrier family 12 member 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_04177</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) + chloride(in) = K(+)(out) + chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:72427"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the SLC12A transporter family. K/Cl co-transporter subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GBA6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015379">
        <property type="term" value="F:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000049">
        <property type="entry name" value="Solute carrier family 12 (potassium/chloride transporter), member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000037">
        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000076">
        <property type="entry name" value="KCL_cotranspt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00930">
        <property type="entry name" value="2a30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF73">
        <property type="entry name" value="KAZACHOC, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01081">
        <property type="entry name" value="KCLTRNSPORT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="127"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="176"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="411"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="540"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="573"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="18">
        <location>
            <begin position="99"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="18">
        <location>
            <begin position="377"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="19">
        <location>
            <begin position="673"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="19">
        <location>
            <begin position="801"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="19">
        <location>
            <begin position="970"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="922"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="935"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="964"/>
            <end position="978"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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</entry>
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</entry>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <source>
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        </source>
    </evidence>
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    </evidence>
    <sequence length="286" mass="32034" checksum="91B044BE56175390" modified="2024-11-27" version="1">MRNPEGSFAVALLLLLLTAASTAADAEPDVEASVSTLPPGPDAPRRMVKRAPTSSFIGMRGKKEEEKDQRAADWIGPDPLDYADMDEDSLYFENGRRLKKAPMSFVGMRGKKYIPFSNRLSDVLHQIEEQRMRENLLQDLFERLAAGDDNVGDMGKRAPTGFTGMRGKRPMSGDEDEDDAMELLQKRAPVNSFLGVRGKKDVSHQHYKRAALSEAYDVRGKKERYADFNSKFVAVRGKKSEQDTGLDTDGGDQQYLVRPWLYLWGDNKRAPSGFQGMRGKRPALVE</sequence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ETR5</accession>
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        <submittedName>
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        </submittedName>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <text evidence="2">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
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        <property type="project" value="UniProtKB-ARBA"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ETS7</accession>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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    </dbReference>
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    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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            <taxon>Insecta</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <sequence length="418" mass="48596" checksum="F938DE5F4DE9F0C4" modified="2024-11-27" version="1">MPPQNKDEDPGMEAILVPKWVKPEIFQDLLKQQVKNYKETNSLRASAGVAKGENYATIMLRVELDVETEDRSQVTKAYMLKIAHDSDAYRKILEKSNMFDTERGMYLKVVPEMEQMYRDVGLEVKFGAQSYEIPTNENYVLLEDLKPQGFKNFDRLQGLDQAHTESALRKFAQWHAASAVRVDTKGPYEERYTKGFFRSPEMINAFFKGSMNSLLKYIEHYEGRETYLKDLLSISEKLVDIVTKIIEPNPEEFNALNHGDGWVNNIMYQYNEKNDILNTFFVDLQLPKWGSVAQDLYYFLISSTSLDIKTSKFDYFIWFYHSELVKHLQLLNYSKPVPTLRGIRNDLSKHSGWGLVCCASIMGFVLLDPIVDDNGDFDKLISEEDSSFKKSLFTNPRFRKHVEVLLPWLQHRGAMEYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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                <person name="Yamamoto D."/>
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        </location>
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        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF90508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1846" mass="199905" checksum="9C4B2FC8E4818F5F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F5L2</accession>
    <name>A0AAU9F5L2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_09437</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F5L2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20993">
        <property type="entry name" value="GH07914P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20993:SF0">
        <property type="entry name" value="GH07914P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043650429" evidence="1">
        <location>
            <begin position="19"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF91070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="259" mass="28976" checksum="B3222A817B9429EF" modified="2024-11-27" version="1">MASQLFLVALLGIALVSATPVPQQAGSANFTSAMTRYLQAFRSVMPCGYAPEGIPVLAPIVTPFYAYDISTDDYSLKGNVSNMRIEGLNNFKILSASDYGKTGQAAYDVIFPKIQILGASEVEGFVNIGGFHLPIRQRDVINEALVDLRFVGSYTFANSLTNSSGLRIVDFKQSIYLADVLMDNWNSLWNISTNNFWNRYSRIFISLGLEEIQPKITDFLYQNYFVPKINELLANVSMEELVNFYQSQAQLWENAHCQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F5L8</accession>
    <name>A0AAU9F5L8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Heterogeneous nuclear ribonucleoprotein D-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_08738</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Musashi family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F5L8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12323">
        <property type="entry name" value="RRM2_MSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000320">
        <property type="entry name" value="RNA-binding protein Musashi 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000314">
        <property type="entry name" value="RNA-binding protein Musashi Rbp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034126">
        <property type="entry name" value="MSI_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF4">
        <property type="entry name" value="FI20028P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="196"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="284"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="118"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="157"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="492"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFF90166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="631" mass="65154" checksum="D6D39B9A30DD89A1" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F5Q9</accession>
    <name>A0AAU9F5Q9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Microtubule-associated protein Jupiter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_07889</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to all microtubule populations.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAP Jupiter family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F5Q9"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033335">
        <property type="entry name" value="JUPITER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34930">
        <property type="entry name" value="GEO05313P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34930:SF2">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN JUPITER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17054">
        <property type="entry name" value="JUPITER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="112"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="208"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="288"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="130"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="219"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="310"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF89048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="342" mass="36327" checksum="7458C7210CA0967D" modified="2024-11-27" version="1">MISNYDISDSKSSSKVLRPPGGGSSDIFGSDMPQTPRNVKNRMVSNIFSVEKDNSVKNTGDNPRRGQKTVDSHSRLFGEPMRPITPGKNHMKSSIPFGQNTETAAAQKLLTNGSSTTNGATANGHYNGKSGSVSSASSSVSSSTENLKMNSGSRSVFIRNMSRAEEFAESQQNKQAINKEGPEVLDIDQPCLDLEVGDVPKDNEIYTESGKRDVRMQSRQDSASNVKQPHSLDKMHNDPDVKEPLSLCPDYGKEAHDPCNARNPITGLGLNGDGVGGLKPVKQKIREGNPVTGEGYRPGGTDYIQAAGSTNGGSNGGNNGNGGNSVVNKNRVPPGGYSSGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F5S2</accession>
    <name>A0AAU9F5S2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein takeout</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DMAD_08876</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TO family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F5S2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.10.30:FF:000001">
        <property type="entry name" value="Takeout-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008:SF32">
        <property type="entry name" value="CIRCADIAN CLOCK-CONTROLLED PROTEIN DAYWAKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044020886" evidence="4">
        <location>
            <begin position="21"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF90331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="261" mass="30545" checksum="7346B5CC016894E8" modified="2024-11-27" version="1">MNKLLTIIWLYCICIGASLAAELPEDIERCRFGDSPCLVRTLNDLIKRYPKGIPEIGLPPLDKYSFPDTSVLDSPYRGPIWMTFHMRDNVHKGFNNATVTHVEGFLQDPTKQQIILKARLPRLVHDATYDMQGRFMLFVMNTTGTLQSDFQNFRVTLTIKVILEYRNNKRYLNIYDMVPVIDLDRLIVWFDNLYRENEDVTIALNNSFNKHWLEFWNELEPALLRSFTAGFTVLLSTVFEKVAYDDMFLPDIDIRIGYRDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F5S5</accession>
    <name>A0AAU9F5S5_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Twist-related protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_07924</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F5S5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000035">
        <property type="entry name" value="Pancreas-specific transcription factor 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF97">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="156"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="79"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF89093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="282" mass="29729" checksum="BDE63720B3D930E8" modified="2024-11-27" version="1">MSRNQLPDSSSSPPISHMPFHNFDDDLINDLPSCIYAGQHQGGGNNGGGGAGGPGGSSGLRLNSNNLRHIQQHYMQHGGENLLDSSTNPMGVHQSNSATPLMCNSPSASSSGGNGGPGGATGAGGGGVGGGGSNPNYGSVGATAASSYKMQRQAANVRERKRIQRSAPTGSINSAFDELRVHVPTFPYEKRLSKIDTLRLAIAYISLLREVLQTDYDPLTYVEKCLRGEIKADRANWNTSDLTARLSWINWENLGVHPGRRTLLTSLALSSEPMCGAHCGMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F624</accession>
    <name>A0AAU9F624_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional adapter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_09794</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF91535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F624"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070461">
        <property type="term" value="C:SAGA-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02335">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000008">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041983">
        <property type="entry name" value="ADA2-like_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016827">
        <property type="entry name" value="Ada2/TADA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056267">
        <property type="entry name" value="Ada2b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055141">
        <property type="entry name" value="TADA2A_B-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374:SF63">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 2-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTOR 2 ADA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22941">
        <property type="entry name" value="TADA2A-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24533">
        <property type="entry name" value="Tri-helical_Ada2b_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25299">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025024">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="12">
        <location>
            <begin position="8"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="14">
        <location>
            <begin position="66"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="13">
        <location>
            <begin position="69"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="11">
        <location>
            <begin position="71"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="319"/>
            <end position="381"/>
        </location>
    </feature>
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        <location>
            <begin position="471"/>
            <end position="494"/>
        </location>
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        </location>
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            <begin position="471"/>
            <end position="488"/>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR025024"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF91535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="573" mass="63798" checksum="B333069CA4263693" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F642</accession>
    <name>A0AAU9F642_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_08210</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="BFF89453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:mRNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="PNRC-like_rgn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026780">
        <property type="entry name" value="PNRC1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15405">
        <property type="entry name" value="PROLINE-RICH NUCLEAR RECEPTOR COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="77"/>
            <end position="147"/>
        </location>
    </feature>
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        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="77"/>
            <end position="115"/>
        </location>
    </feature>
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        <location>
            <begin position="132"/>
            <end position="144"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF89453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="285" mass="29435" checksum="230EACD300221462" modified="2024-11-27" version="1">MTYSHNSVRNNIRMTTASATKAIRLKKGAAGTAASPSASANPSPTSTASAPTTPLQSLSAAASPTTCSGLGLSALTTSSTITTPNSRQQRQQQSQSAGGAQRSRQQPQQRQSPQQIAGGGGGAFFFANNNGSASKRNGGRSPQGTGMVVRQSPATILGGGFSPSMAINGGSARKQRKSPPTIGAFGASPQQQHPLIPTALTHFAGSKCFDAPAPTALPKPPQHWTLCKSEEKLASSAGPALQSLMRTGGEYLSSPRFHLGGKSSKRNLLDDFDTHNLKLLLNVQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FFN2</accession>
    <name>A0AAU9FFN2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MICOS complex subunit MIC13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_12073</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
            <topology evidence="1 8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the MICOS complex subunit Mic13 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FFN2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044284">
        <property type="term" value="C:mitochondrial crista junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026769">
        <property type="entry name" value="Mic13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31816">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31816:SF3">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15884">
        <property type="entry name" value="QIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043616939" description="MICOS complex subunit MIC13" evidence="9">
        <location>
            <begin position="19"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFF94446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="122" mass="14209" checksum="16131453868DD32F" modified="2024-11-27" version="1">MVLMVIAKLGVVAGVAFATKQLGVWESPEHSAVLLENARESLRPYAQDLKDRICCWKCQEKCMEEFEPKPWRESFVDSWNDTIKKAFHFLNQVPTNFHHFMEDVEKAVTDLVKEKDQKIQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FFN5</accession>
    <name>A0AAU9FFN5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_12123</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FFN5"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFF94543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="28282" checksum="1EB3A9603FF75351" modified="2024-11-27" version="1">MPGTPIVCVCDYWQRRHYYNCSPPSPDAHTTKANCAFYKKRQLRRLRLKVNAINGNYYLREFVQQRVLAFRLQRHHGVQLIWLDFEPPERDTIDHKWAQILAHTIREHIEVQNLMSWLSTLGGGFSALGEQFERCAKTAGKISLQQLKIGLRLGDPCLQARCKLYYSISLIQRGHLRAAKHLICDQQQFARVHREKDQRLVRMCQGIWMRLTYEYKLRRQRVEQPQLGLESQSIPSQILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FFT7</accession>
    <name>A0AAU9FFT7_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ABC-type glutathione-S-conjugate transporter</fullName>
            <ecNumber evidence="13">7.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_12166</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>leukotriene C4(in) + ATP + H2O = leukotriene C4(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:38964"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.3" evidence="13"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015431">
        <property type="term" value="F:ABC-type glutathione S-conjugate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18595">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000812">
        <property type="entry name" value="multidrug resistance-associated protein 1 isoform X15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000074">
        <property type="entry name" value="Multidrug resistance-associated protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000041">
        <property type="entry name" value="Multidrug-Resistance like protein 1, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005292">
        <property type="entry name" value="MRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00957">
        <property type="entry name" value="MRP_assoc_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="477"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="561"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="593"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="992"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1036"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1106"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1134"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1219"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="18">
        <location>
            <begin position="334"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="651"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="18">
        <location>
            <begin position="996"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="1313"/>
            <end position="1547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="928"/>
            <end position="957"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFF94562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1552" mass="173459" checksum="268E76AE21CE70F5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FFU1</accession>
    <name>A0AAU9FFU1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Chromodomain-helicase-DNA-binding protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_12251</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000791">
        <property type="term" value="C:euchromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18668">
        <property type="entry name" value="CD1_tandem_CHD5-9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18663">
        <property type="entry name" value="CD2_tandem_CHD5-9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17995">
        <property type="entry name" value="DEXHc_CHD6_7_8_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000001">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 8 isoform X4"/>
        <property type="match status" value="1"/>
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        <location>
            <begin position="4400"/>
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        </location>
    </feature>
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        <location>
            <begin position="4470"/>
            <end position="4481"/>
        </location>
    </feature>
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            <end position="4510"/>
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            <begin position="4638"/>
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            <begin position="4658"/>
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        <location>
            <begin position="4668"/>
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        <location>
            <begin position="4683"/>
            <end position="4705"/>
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            <begin position="4834"/>
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            <begin position="4964"/>
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            <begin position="5127"/>
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        <location>
            <begin position="5602"/>
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        <location>
            <begin position="5801"/>
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        <location>
            <begin position="5853"/>
            <end position="5862"/>
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        <location>
            <begin position="5869"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFF94676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="5888" mass="627119" checksum="FCF0BD597D556E9C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FFU3</accession>
    <name>A0AAU9FFU3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mothers against decapentaplegic homolog</fullName>
            <shortName evidence="8">MAD homolog</shortName>
            <shortName evidence="8">Mothers against DPP homolog</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">SMAD family member</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_12079</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the dwarfin/SMAD family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF94454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071144">
        <property type="term" value="C:heteromeric SMAD protein complex"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0070411">
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
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        <property type="term" value="P:cell differentiation"/>
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    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0050793">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MH2_SMAD_1_5_9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013790">
        <property type="entry name" value="Dwarfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
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    </dbReference>
    <dbReference type="InterPro" id="IPR013019">
        <property type="entry name" value="MAD_homology_MH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001132">
        <property type="entry name" value="SMAD_dom_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036578">
        <property type="entry name" value="SMAD_MH1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703:SF61">
        <property type="entry name" value="PROTEIN MOTHERS AGAINST DPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703">
        <property type="entry name" value="SMAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03166">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00524">
        <property type="entry name" value="DWB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56366">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51075">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51076">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MH1" evidence="10">
        <location>
            <begin position="26"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="MH2" evidence="11">
        <location>
            <begin position="261"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="192"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="229"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF94454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="455" mass="50603" checksum="DEDDA88B5B54D114" modified="2024-11-27" version="1">MDTDDVESNTSSAMSTLGTLFSFTSPAVKKLLGWKQGDEEEKWAEKAVDSLVKKLKKRKGAIEELERALSCPGQPSKCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLEICQYPFSAKQKEVCINPYHYKRVESPVLPPVLVPRHSEFAPGHSMLQFNHVAEPSMPHNVSYSNTGFNSHSLSNSNTSVGSPSSVNSNPNSPYDSLAGTPPPAYSPSEDGNSNNPNDGNQLLDAQMGDVAQVSYSEPAFWASIAYYELNCRVGEVFHCNNNSVIVDGFTNPSNNSDRCCLGQLSNVNRNSTIENTRRHIGKGVHLYYVTGEVYAECLSDSAIFVQSRNCNYHHGFHPSTVCKIPPGCSLKIFNNQEFAQLLSQSVNNGFEAVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNAISSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FFU6</accession>
    <name>A0AAU9FFU6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_12163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FFU6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="799"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
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        </location>
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            <end position="360"/>
        </location>
    </feature>
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            <begin position="371"/>
            <end position="388"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF94558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="855" mass="93210" checksum="67BB5760E21985DD" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FFU8</accession>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001500889">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="303"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
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        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
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        <location>
            <begin position="96"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <end position="177"/>
        </location>
    </feature>
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        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF94583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="377" mass="41479" checksum="042DEDC8E8FDDCB0" modified="2024-11-27" version="1">MDTKSNDDSAGSDSLDKSMQIKCVMVETTSDAMATGPTVGEESDNCSRDSAARDMGDKSASPSPTGAMQREQQAEQKTDAVSSMGDGDSSSQTGASSNCSSNTSPSSIVSCTAFETKVKQISQNLKETTLAEPVAKSEPTTSDAAASDAATSDAATSDTVTSDAATSDTATNDAATSDARRVKKVRFHPDVKENDGGTWAKKKRSPQSSEADSSANCDGMDLDEDEECDEGDEEPEEEFDMAKTIAEAEDYLKEHPLTFVRRAEQNGDTLNDGFDTIDDMPPVVPKEPYDNDVDFYRKIEPENGIERVLGRETKAGKVEYLLRYENQGGLFWESEEFIRRMCPTLLKEYEKNRERRQQRLMHHVAKRQTMRQRYTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:dendrite self-avoidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002164">
        <property type="entry name" value="Basigin, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075">
        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF104">
        <property type="entry name" value="BASIGIN, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043414896" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="115"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF94618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="259" mass="29092" checksum="29B051BD76FB567A" modified="2024-11-27" version="1">MEAKFLASALSFLSIFLAIYAQSLDTLVPNYDNVEHRMKFYDIRGPLVLSCKSESTDPLIWKKNHTNVMNVDVLKGRFKLINDEKKFIIDRAEAHDDGLYSCEINGESRDIHAVARVVVRVPSNTAVVEGEKMSVTCSVLGTAPQLSWTFGNVTLKNNTDRFVLKAENNIENAILTMDNVTLDDRGEYKCIGRNAANDYGNSTVASDFTTVRVKGKFAALWPFLGICAEVLILCLIILIYEKRRNKSELEESDTDPQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FG40</accession>
    <name>A0AAU9FG40_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Androgen-induced gene 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_12241</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>12-(9Z-hexadecenoyloxy)-octadecanoate + H2O = 12-hydroxyoctadecanoate + (9Z)-hexadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52072"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
            <dbReference type="ChEBI" id="CHEBI:136312"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:52073"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>12-(9Z-octadecenoyloxy)-octadecanoate + H2O = 12-hydroxyoctadecanoate + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52060"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
            <dbReference type="ChEBI" id="CHEBI:136302"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:52061"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>12-hexadecanoyloxy-octadecanoate + H2O = 12-hydroxyoctadecanoate + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52056"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83677"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:52057"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>12-octadecanoyloxy-octadecanoate + H2O = 12-hydroxyoctadecanoate + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
            <dbReference type="ChEBI" id="CHEBI:136330"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:52081"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>13-(9Z-hexadecenoyloxy)-octadecanoate + H2O = 13-hydroxy-octadecanoate + (9Z)-hexadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52076"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:136304"/>
            <dbReference type="ChEBI" id="CHEBI:136315"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:52077"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>13-(9Z-octadecenoyloxy)-octadecanoate + H2O = 13-hydroxy-octadecanoate + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:136303"/>
            <dbReference type="ChEBI" id="CHEBI:136304"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:52065"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>13-octadecanoyloxy-octadecanoate + H2O = 13-hydroxy-octadecanoate + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52084"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:136304"/>
            <dbReference type="ChEBI" id="CHEBI:136335"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:52085"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>9-(9Z-hexadecenoyloxy)-octadecanoate + H2O = (9Z)-hexadecenoate + 9-hydroxy-octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52068"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
            <dbReference type="ChEBI" id="CHEBI:136309"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:52069"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>9-(9Z-octadecenoyloxy)-octadecanoate + H2O = 9-hydroxy-octadecanoate + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52048"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:136282"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:52049"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>9-hexadecanoyloxy-octadecanoate + H2O = 9-hydroxy-octadecanoate + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52052"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83670"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:52053"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>9-octadecanoyloxy-octadecanoate + H2O = 9-hydroxy-octadecanoate + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
            <dbReference type="ChEBI" id="CHEBI:136373"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:52097"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FG40"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006838">
        <property type="entry name" value="ADTRP_AIG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989">
        <property type="entry name" value="ANDROGEN-INDUCED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989:SF16">
        <property type="entry name" value="AT02829P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04750">
        <property type="entry name" value="Far-17a_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFF94663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="245" mass="27851" checksum="545637A239C05BD1" modified="2024-11-27" version="1">MAKPKTKSKSKGSTSPGFNKAVQLVLHLTAVLQFSYGVYIFHNIQMPNTVPFAGKFKFLTFICAIIQALYYIVSLVNDFVGTNELAPKRPPAIRQFKDWLMATLAFPVALNVGVTFWSLYAIDRELVFPKALDPVFPSWLNHILHTNIVVFIVLELFTSYRAYPKRSKGLTGLTVFMGAYLVWIHIVKHYSGVWVYPVLEVLQLPQRIVFFVTVIGFTLALYLVGEFLNNTVWAKEVKLAKRKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FG44</accession>
    <name>A0AAU9FG44_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_12224</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FG44"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004742">
        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000010">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000003">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045257">
        <property type="entry name" value="E2/Pdx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151:SF90">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="6">
        <location>
            <begin position="83"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="179"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF94638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="462" mass="49480" checksum="E743E20D83337FC9" modified="2024-11-27" version="1">MLRSLATTRNELGALRAVLLRRPATACLRRGDGQVGVRALSTRLLTNARKLQASRSILVNSRPRSVNAWSYNFARNYASLPEHLRVPLPALSPTMERGSIVSWEKKEGDKLNEGDLLCEIETDKATMGFETPEEGYLAKILIPGGTKDVPIGQLLCIIVNDQASVAAFKDFKDDAPAAAPAAAAAPPPPPPPAAAAPAPVAAPPPAAAPAPAGQQPAAAAKSAPPKAAPGARYTDIPVTTMRAVIAKRLLESKTQLPHYYVTAQCQVDNLLKFRAKVNKKYEKQGARVSVNDFIIKATAIASLKVPEANSAWMDSVIRQYDDVDVSVAVSTDKGLITPIIFGADRKGVLDISKDVKELAAKARANKLAPHEFQGGTISVSNLGMFGVNQFCAVINPPQSCILAIGTTTKQLVLDPDSLKGFKEINLLTVTLSADHRVVDGAVAARWLQHFRDYIEDPQNMIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FG46</accession>
    <name>A0AAU9FG46_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Piezo-type mechanosensitive ion channel component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_12317</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PIEZO (TC 1.A.75) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027272">
        <property type="entry name" value="Piezo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031334">
        <property type="entry name" value="Piezo_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056770">
        <property type="entry name" value="Piezo_THU9_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056769">
        <property type="entry name" value="Piezo_TM1-24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031805">
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Muscomorpha</taxon>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Yamamoto D."/>
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        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043975672" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="115"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFF94617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="259" mass="29101" checksum="3E8051BD76FB567A" modified="2024-11-27" version="1">MEAKFLASALSFLSIFLAIYAQSLDTLVPNYDNVEHRMKFYDIRGPLVLSCKSESTDPLIWKKNHTNVMNVDVLKGRFKLINDEKKFIIDRAEAHDDGLYSCEINGESRDIHAVARVVVRVPSNTAVVEGEKMSVTCSVLGTAPQLSWTFGNVTLKNNTDRFVLKAENNIENAILTMDNVTLDDRGEYKCIGRNAANDYGNSTVASDFTTVRVKGKFAALWPFLGICAEVLILCLIILIYEKRRNKSELEESDTDPQEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FG60</accession>
    <name>A0AAU9FG60_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dedicator of cytokinesis protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_12234</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08697">
        <property type="entry name" value="C2_Dock-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11694">
        <property type="entry name" value="DHR2_DOCK_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13267">
        <property type="entry name" value="PH_DOCK-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.740:FF:000001">
        <property type="entry name" value="dedicator of cytokinesis protein 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000115">
        <property type="entry name" value="Uncharacterized protein, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037809">
        <property type="entry name" value="C2_Dock-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
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    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021816">
        <property type="entry name" value="DOCK_C/D_N"/>
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        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
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        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
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        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
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        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
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        <property type="entry name" value="DEDICATOR OF CYTOKINESIS DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317:SF26">
        <property type="entry name" value="ZIZIMIN, ISOFORM K"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DHR-2_Lobe_B"/>
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    </dbReference>
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        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DOCK_C-D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="281"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="C2 DOCK-type" evidence="5">
        <location>
            <begin position="787"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="6">
        <location>
            <begin position="1803"/>
            <end position="2234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="107"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1548"/>
            <end position="1576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2237"/>
            <end position="2258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="125"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1564"/>
            <end position="1576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="2238"/>
            <end position="2249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF94653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2258" mass="256508" checksum="8230D91FA98D64A6" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FG61</accession>
    <name>A0AAU9FG61_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Chromodomain-helicase-DNA-binding protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_12251</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000791">
        <property type="term" value="C:euchromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="CD1_tandem_CHD5-9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DEXHc_CHD6_7_8_9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SF2_C_SNF"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000001">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 8 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000003">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 8 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000015">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006576">
        <property type="entry name" value="BRK_domain"/>
    </dbReference>
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        <property type="entry name" value="BRK_sf"/>
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    <dbReference type="InterPro" id="IPR051493">
        <property type="entry name" value="CHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056342">
        <property type="entry name" value="HTH_CHD6-9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46850">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46850:SF1">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07533">
        <property type="entry name" value="BRK"/>
        <property type="match status" value="1"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFF94699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="6044" mass="646565" checksum="7BDAB5377A36146E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWT8</accession>
    <name>A0AAU9FWT8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_00244</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FWT8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19142">
        <property type="entry name" value="AKR_AKR6B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000032">
        <property type="entry name" value="Hyperkinetic, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005983">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01293">
        <property type="entry name" value="Kv_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150:SF2">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01577">
        <property type="entry name" value="KCNABCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="11">
        <location>
            <begin position="372"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="13"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="30"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="83"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="98"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="137"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="167"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG00193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="702" mass="78947" checksum="F245C88238F493B3" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWU1</accession>
    <name>A0AAU9FWU1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase</fullName>
            <ecNumber evidence="6">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_00041</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 9">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="8">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="6"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFF99916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FWU1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042592">
        <property type="term" value="P:homeostatic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16212">
        <property type="entry name" value="EFh_NorpA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13361">
        <property type="entry name" value="PH_PLC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08591">
        <property type="entry name" value="PI-PLCc_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000024">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1230.10:FF:000005">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.240:FF:000001">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000008">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1230.10">
        <property type="entry name" value="Phospholipase C beta, distal C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016280">
        <property type="entry name" value="PLC-beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042531">
        <property type="entry name" value="PLC-beta_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037862">
        <property type="entry name" value="PLC-beta_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053945">
        <property type="entry name" value="PLCB1-4-like_EFh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF36">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE BETA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17787">
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <source>
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    <sequence length="322" mass="34060" checksum="B75A7E6693FA039E" modified="2024-11-27" version="1">MDNSIVVEENGNSASSGSLDVITAAAQAAAAAAAATSAAQQQSQNPQSTTAASAGNTNNSAGTATVLTTTANCNIQYPIQALTQHGLQVQSVIQANPSGVIQTAAGTQQHQALAAATASAMHKGVGVVYVAKPPNSTVIHTTAGNAVQIPPTFPCKIKPEPNSQHQEDSDESLSDDDSQHHRSELTRRPSYNKIFTEISGPDISGSSLQMGSDGLLGSQLAAGGPGGNSANSPLLHMAPDPAYYLSNRIPYNTNNSGIAEDQTRKREIRLQKNREAARECRRKKKEYIKCLENRVAVLENQNKALIEELKSLKELYCQTKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWV2</accession>
    <name>A0AAU9FWV2_DROMD</name>
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        <submittedName>
            <fullName evidence="8">Serine/threonine-protein kinase S6KL</fullName>
        </submittedName>
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        <name evidence="8" type="ORF">DMAD_00180</name>
    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pk17E, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="192"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="146"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFG00105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="394" mass="43668" checksum="771CC3937DCA1ED2" modified="2024-11-27" version="1">MGNSQTRHASRRTSQSTREAAAAQRHELIETEAATTATASASNSNQQPEAAPQGRRQQSLPSTSFGCGGSSRKSPHRPPPPAQALSVPEPEPVASNSIGVVTAEAETQAAATAATTVGLQQLSTWSLGSLSGGRLNWSFSAARNSFRHRNKATRKSSSSLQGFRRRKTQWHRPLTNSIFNSHFKEASRNDQFQIEHLVAKGAFGVVFKVCNRTDTSSCYALKVLKKSKLIEDNSVRQIKDEADIQKVCGHHPFIVQQFDLWQNRHNLHILSEYIPNGELFSKIVDFSIDLVRLYIGEIALAIDFLHNAGIIYRDAKPENILLTQQFHIKLTDFGLSKWLKLGANTRTICGTFKYMAPEILCGEPYGHAVDWWALGVIACQMLTQKVSEQKSDNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWV3</accession>
    <name>A0AAU9FWV3_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Rho GTPase-activating protein 26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_00128</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="BAR-PH_GRAF_family"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="CDD" id="cd11882">
        <property type="entry name" value="SH3_GRAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000411">
        <property type="entry name" value="Blast:Rho GTPase-activating protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000006">
        <property type="entry name" value="Rho GTPase activating protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000001">
        <property type="entry name" value="Rho GTPase-activating protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000055">
        <property type="entry name" value="rho GTPase-activating protein 26 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
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    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047234">
        <property type="entry name" value="GRAF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
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        <property type="entry name" value="PH_GRAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
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    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552">
        <property type="entry name" value="OLIGOPHRENIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552:SF1">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN GRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="271"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="10">
        <location>
            <begin position="402"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="950"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="601"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="670"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="226"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="601"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG00036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1012" mass="113349" checksum="04FC99E6E87490DF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWW1</accession>
    <name>A0AAU9FWW1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycine-rich cell wall structural protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_00053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFF99931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FWW1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043493739" evidence="1">
        <location>
            <begin position="20"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF99931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="261" mass="25260" checksum="EA66B6A7A6A7F885" modified="2024-11-27" version="1">MKCFFVLVAASLMLSSVWAEDTAALEPAADAPLADQDQVSAASNYAPLQEKKQEKRYVGGYGGYGGYSGYGGGGYGSPLGAGYGGAYGAAGYGSGVGLGAGAGLGVDSYYNPYSSRSLGGLDGSAVAGYGGYGSALGGYGSALGGYGSSLGGYGSSLGGYGSSLGGYGSGLGGVGGYGSYNSNPYALSSYYNGRGLGSGIGTGTGYPYYNTRFGAGGYPSSYYTSNYGNSVVGGGLGALAGGVPPIGSGYNYGAGISGTVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FX03</accession>
    <name>A0AAU9FX03_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">28 kDa heat- and acid-stable phosphoprotein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DMAD_00063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFF99944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FX03"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039876">
        <property type="entry name" value="HAP28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22055">
        <property type="entry name" value="28 KDA HEAT- AND ACID-STABLE PHOSPHOPROTEIN PDGF-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF99944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="186" mass="21100" checksum="6E0BF097EB75F674" modified="2024-11-27" version="1">MPRQKQVTHKGKSREFTPVEEMQQDWEEHLKETSLSSDEDESSSDDDSHGQHGKTLRPKGVQSLIEISNPNRSKSANAWKDEICDDPLVVSPPKPGRRREMRRSPNIKLSGAIQADLDRLAVIRKERQQAAQRRFDTKKGDRDRSSSKSSNGGSRYLKKSSHDRGAYLQNSAGPQKPNRKSVLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FX50</accession>
    <name>A0AAU9FX50_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Interference hedgehog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_00393</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mediates response to the active Hedgehog (Hh) protein signal in embryos, functioning upstream or at the level of patched (ptc).</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer. Heterotetramer; 2 iHog chains bind 2 hh chains when facilitated by heparin, heparin is required to promote high-affinity interactions between hh and iHog.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the immunoglobulin superfamily. IHOG family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FX50"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001747">
        <property type="entry name" value="Interference hedgehog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001773">
        <property type="entry name" value="Interference hedgehog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF33">
        <property type="entry name" value="BROTHER OF IHOG, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 18" id="KW-0732">Signal</keyword>
    <keyword evidence="3 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043414909" description="Interference hedgehog" evidence="18">
        <location>
            <begin position="21"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="749"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="29"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="138"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="239"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="330"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="617"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="428"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="538"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="875"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="543"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="958"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="994"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1036"/>
            <end position="1052"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="BFG00379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1052" mass="115190" checksum="0D4F4BA4671713B9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FX52</accession>
    <name>A0AAU9FX52_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extensin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_00205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FX52"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043885640" evidence="2">
        <location>
            <begin position="28"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="355"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="426"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="575"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFG00141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="631" mass="65519" checksum="A265FBD5E0B2F927" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FX65</accession>
    <name>A0AAU9FX65_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP-1 complex subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_00447</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FX65"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFG00156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="3288" mass="360592" checksum="8592A9B31F476237" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FX72</accession>
    <name>A0AAU9FX72_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Voltage-gated potassium channel subunit beta-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_00244</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FX72"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19142">
        <property type="entry name" value="AKR_AKR6B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000032">
        <property type="entry name" value="Hyperkinetic, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005983">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01293">
        <property type="entry name" value="Kv_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150:SF2">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01577">
        <property type="entry name" value="KCNABCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="10">
        <location>
            <begin position="63"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG00198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="393" mass="44117" checksum="2842BF521CD80D5B" modified="2024-11-27" version="1">MCRAPIASLDCMEEFSGTGGHLDFGRSISLGSNPALPLRHGSTPTPGLRYKNLGKSGLRISNVGLGTWPVFSPGVSDEQAEAILKLAIESGINLFDISEAHSETEIGKILQRAGWKRTAYVITTKVYWSTKSEERGLSRKHIIECVRASLQRLQLQYIDIVIIHKADPMCPMEEVVRAMSYVIQQGWAMYWGTARWSQVEIMEAYTNCRQFNCITPIVEQSEYHMFCREKCELYLPEMYNKIGVGLMAWGPLSMALSDTQNGDKIFLPKGSFKTKSFSWTEDEINRNAALSPQGSWGKDRIEEGRRHCDRLRDLAALAEKLGCSPTQLSIAWSLKHEPVQCLLLGATSAEQLHQSLQSLQLLPRLSSSVMLELERILENKPVRPPMISTLALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FX78</accession>
    <name>A0AAU9FX78_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_00244</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FX78"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19142">
        <property type="entry name" value="AKR_AKR6B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000032">
        <property type="entry name" value="Hyperkinetic, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005983">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01293">
        <property type="entry name" value="Kv_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150:SF2">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01577">
        <property type="entry name" value="KCNABCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="11">
        <location>
            <begin position="380"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="13"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="30"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="83"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="98"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="137"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="167"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG00196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="709" mass="79603" checksum="68AAE4C65FD0DAB7" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FX80</accession>
    <name>A0AAU9FX80_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphorylase b kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_00452</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Glycan biosynthesis; glycogen metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Lipid-anchor</topology>
            <orientation evidence="2 13">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the phosphorylase b kinase regulatory chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005964">
        <property type="term" value="C:phosphorylase kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000004">
        <property type="entry name" value="Phosphorylase b kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045583">
        <property type="entry name" value="KPBA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008734">
        <property type="entry name" value="PHK_A/B_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749:SF7">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19292">
        <property type="entry name" value="KPBB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="10 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 13" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="11 13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 13" id="KW-0636">Prenylation</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH15-like" evidence="15">
        <location>
            <begin position="8"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphorylase b kinase regulatory subunit alpha/beta C-terminal" evidence="16">
        <location>
            <begin position="1043"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="825"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="861"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="834"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="861"/>
            <end position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF19292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG00464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1313" mass="146673" checksum="4931EE99AE76919B" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0D0</accession>
    <name>A0AAU9G0D0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Sodium/potassium/calcium exchanger 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Na(+)/K(+)/Ca(2+)-exchange protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Solute carrier family 24 member 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">DMAD_00965</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Calcium, potassium:sodium antiporter that transports 1 Ca(2+) and 1 K(+) to the melanosome in exchange for 4 cytoplasmic Na(+). Involved in pigmentation, possibly by participating in ion transport in melanosomes. Predominant sodium-calcium exchanger in melanocytes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in) + 4 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:69967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Melanosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G0D0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000009">
        <property type="entry name" value="sodium/potassium/calcium exchanger 5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000015">
        <property type="entry name" value="sodium/potassium/calcium exchanger 5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00367">
        <property type="entry name" value="calcium/sodium antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF70">
        <property type="entry name" value="ZYDECO, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="18 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0915">Sodium</keyword>
    <keyword evidence="19" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="12" id="KW-0769">Symport</keyword>
    <keyword evidence="9 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="488"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="26">
        <location>
            <begin position="33"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="26">
        <location>
            <begin position="351"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00033627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00058187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00069887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00081356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00082809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="BFG01137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="521" mass="55814" checksum="A42F9DAD92A23108" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0D4</accession>
    <name>A0AAU9G0D4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Coronin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_01105</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">F-actin regulator involved in anterograde Golgi to endosome transport: upon ubiquitination via 'Lys-33'-linked ubiquitin chains by the BCR(KLHL20) E3 ubiquitin ligase complex, interacts with EPS15 and localizes to the trans-Golgi network, where it promotes actin polymerization, thereby facilitating post-Golgi trafficking. May play a role in the maintenance of the Golgi apparatus morphology.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the WD repeat coronin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G0D4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000076">
        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000362">
        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015505">
        <property type="entry name" value="Coronin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015048">
        <property type="entry name" value="DUF1899"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856:SF20">
        <property type="entry name" value="CORONIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08953">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16300">
        <property type="entry name" value="WD40_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01166">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01167">
        <property type="entry name" value="DUF1900"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="DUF1899" evidence="11">
        <location>
            <begin position="4"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="75"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1899" evidence="11">
        <location>
            <begin position="651"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="775"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="818"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="432"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="484"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="442"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="498"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="516"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="528"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="546"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU280818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFG01323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1114" mass="123081" checksum="F83C90C5E5800F65" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0D6</accession>
    <name>A0AAU9G0D6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Tektin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DMAD_01599</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G0D6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFG01967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="109" mass="12905" checksum="2D4E7B4DDED0FA8B" modified="2024-11-27" version="1">MLCWANEDHFLSQRYPNPQQKAPETLKNLLLRSSVPIVSDSEVVESTEMAPLKGQEVTKFHKHSFLENRIFDHRLVKDNLLDKWPTADMNEYHERKDHRNLMESYVPIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0E3</accession>
    <name>A0AAU9G0E3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5-demethoxyubiquinone hydroxylase, mitochondrial</fullName>
            <shortName evidence="8">DMQ hydroxylase</shortName>
            <ecNumber evidence="8">1.14.99.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ubiquinone biosynthesis monooxygenase COQ7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Coq7</name>
        <name evidence="9" type="ORF">DMAD_01108</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the hydroxylation of 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) during ubiquinone biosynthesis. Has also a structural role in the COQ enzyme complex, stabilizing other COQ polypeptides. Involved in lifespan determination in a ubiquinone-independent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol + AH2 + O2 = a 3-demethylubiquinol + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:50908"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="1.14.99.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="8">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of a multi-subunit COQ enzyme complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the COQ7 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.60" evidence="8"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G0E3"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008682">
        <property type="term" value="F:3-demethoxyubiquinol 3-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008340">
        <property type="term" value="P:determination of adult lifespan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000377">
        <property type="term" value="P:regulation of reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01042">
        <property type="entry name" value="DMQH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.10:FF:000025">
        <property type="entry name" value="5-demethoxyubiquinone hydroxylase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01658">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011566">
        <property type="entry name" value="Ubq_synth_Coq7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237:SF4">
        <property type="entry name" value="5-DEMETHOXYUBIQUINONE HYDROXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237">
        <property type="entry name" value="COENZYME Q10 BIOSYNTHESIS PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03232">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <source>
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    <sequence length="4922" mass="542781" checksum="BE90EF415F91E0D2" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0E9</accession>
    <name>A0AAU9G0E9_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Diacylglycerol lipase-alpha</fullName>
            <ecNumber evidence="21">3.1.1.116</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="32">Neural stem cell-derived dendrite regulator</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">Sn1-specific diacylglycerol lipase alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="36" type="ORF">DMAD_00913</name>
    </gene>
    <organism evidence="36 37">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="36 37" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="36 37">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + H2O = 2-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38511"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:38512"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>1-(9Z-octadecenoyl)-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + H2O = 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38515"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:52392"/>
            <dbReference type="ChEBI" id="CHEBI:75449"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:38516"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1-(9Z-octadecenoyl)-2-(9Z,12Z-octadecadienoyl)-sn-glycerol + H2O = 2-(9Z,12Z-octadecadienoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38523"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75450"/>
            <dbReference type="ChEBI" id="CHEBI:75457"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:38524"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>1-(9Z-octadecenoyl)-2-O-(5Z,8Z,11Z,14Z-eicosatetraenyl)-sn-glycerol + H2O = 2-O-(5Z,8Z,11Z,14Z)-eicosatetraenylglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38527"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75913"/>
            <dbReference type="ChEBI" id="CHEBI:75914"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:38528"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1-(9Z-octadecenoyl)-2-octadecanoyl-sn-glycerol + H2O = 2-octadecanoylglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38519"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75448"/>
            <dbReference type="ChEBI" id="CHEBI:75456"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:38520"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + H2O = 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:52392"/>
            <dbReference type="ChEBI" id="CHEBI:75728"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:38508"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a 1,2-diacyl-sn-glycerol + H2O = a 2-acylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33275"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17389"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.116"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:33276"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="29">Interacts (via C-terminal) with CAMK2A; leading to the phosphorylation and inhibition of DAGLA enzymatic activity. Interacts (via PPXXF motif) with HOMER1 and HOMER2; this interaction is required for DAGLA membrane localization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Early endosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Postsynaptic density membrane</location>
            <topology evidence="22">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.116" evidence="21"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG01074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032591">
        <property type="term" value="C:dendritic spine membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004465">
        <property type="term" value="F:lipoprotein lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046340">
        <property type="term" value="P:diacylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098921">
        <property type="term" value="P:retrograde trans-synaptic signaling by endocannabinoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000015">
        <property type="entry name" value="Sn1-specific diacylglycerol lipase alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052214">
        <property type="entry name" value="DAG_Lipase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45792">
        <property type="entry name" value="DIACYLGLYCEROL LIPASE HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45792:SF8">
        <property type="entry name" value="DIACYLGLYCEROL LIPASE-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 34" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0770">Synapse</keyword>
    <keyword evidence="7 34" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 34" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="18"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="55"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="102"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="135"/>
            <end position="157"/>
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    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="35">
        <location>
            <begin position="400"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
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            <begin position="693"/>
            <end position="722"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="1043"/>
            <end position="1077"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="33">
        <location>
            <begin position="693"/>
            <end position="711"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004332"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004520"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00024531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00026104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00037872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00050486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00050709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00050861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00052106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00052463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00063298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00071957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00081678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00082132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="EMBL" id="BFG01074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1305" mass="144731" checksum="197ED4FC95D0A960" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G0F0</accession>
    <name>A0AAU9G0F0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">WW domain binding protein VOPP1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Vesicular, overexpressed in cancer, prosurvival protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DMAD_00994</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Endomembrane system</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Late endosome membrane</location>
            <topology evidence="13">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VOPP1/ECOP family.</text>
    </comment>
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        <property type="protein sequence ID" value="BFG01177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026229">
        <property type="entry name" value="VOPP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14971">
        <property type="entry name" value="VESICULAR, OVEREXPRESSED IN CANCER, PROSURVIVAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14971:SF2">
        <property type="entry name" value="VESICULAR, OVEREXPRESSED IN CANCER, PROSURVIVAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043717588" description="WW domain binding protein VOPP1" evidence="19">
        <location>
            <begin position="18"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="493"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="559"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="658"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="565"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="686"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="720"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BFG01177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="731" mass="75709" checksum="53D16037EFF87679" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G8T3</accession>
    <name>A0AAU9G8T3_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Microtubule-actin cross-linking factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_03839</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:intermediate filament"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <source>
            <dbReference type="EMBL" id="BFG04338.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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</entry>
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            <taxon>Diptera</taxon>
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                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    <sequence length="5138" mass="582040" checksum="B473D969559CABDE" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G8U3</accession>
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        <submittedName>
            <fullName evidence="19">Protein transport protein Sec24A</fullName>
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        <name evidence="19" type="ORF">DMAD_03466</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="19 20">RF1</strain>
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        <subcellularLocation>
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        <text evidence="4">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
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        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
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        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
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    </dbReference>
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        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
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        <property type="entry name" value="Helical domain of Sec23/24"/>
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        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="523"/>
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            <begin position="596"/>
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            <begin position="837"/>
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            <begin position="932"/>
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            <begin position="1070"/>
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            <begin position="33"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00626"/>
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        <source>
            <dbReference type="Pfam" id="PF04810"/>
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        <source>
            <dbReference type="Pfam" id="PF04811"/>
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        <source>
            <dbReference type="Pfam" id="PF04815"/>
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        <source>
            <dbReference type="Pfam" id="PF08033"/>
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        <source>
            <dbReference type="EMBL" id="BFG04519.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
    <sequence length="1195" mass="130842" checksum="979AB689365EB67E" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G8U4</accession>
    <name>A0AAU9G8U4_DROMD</name>
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        <submittedName>
            <fullName evidence="13">Probable ascorbate-specific transmembrane electron transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_03511</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G8U4"/>
    <dbReference type="SMR" id="A0AAU9G8U4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Cytochrome b reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CYB561/CYBRD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106">
        <property type="entry name" value="CYTOCHROME B561-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106:SF24">
        <property type="entry name" value="NO EXTENDED MEMORY, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="147"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="12">
        <location>
            <begin position="41"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFG04574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="267" mass="30247" checksum="6339D1D119770A8B" modified="2024-11-27" version="1">MRDRHLAKMPRGGTTTARYEEDPDNAWNCCSWCEYLLIVILSTILLVGVLVLTLFWVMFYRDGFAWSESPKQQFNLHPILMIAGFVTLSGFSILIYRLCRCVKHIYVKLIHMFFHAIAIPCIALGFLSVFDSHEALQKVNFYSLHSWLGFVTMGMFVLQFVIGFFSFLVMLCCENKTYSCRSAMVPIHASLGLANFWLAIATSVTGLIEKERETVAETNVSAENKLIEHYITSAIGITLIFIGIIVTFAVRRSNAPASAKVYVTERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G8U7</accession>
    <name>A0AAU9G8U7_DROMD</name>
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        <recommendedName>
            <fullName evidence="19">Microtubule-actin cross-linking factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DMAD_03839</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="microtubule-actin cross-linking factor 1 isoform X13"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041615">
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036534">
        <property type="entry name" value="GAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049538">
        <property type="entry name" value="PCN-like_spectrin-like_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043197">
        <property type="entry name" value="Plakin"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001101">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RANBP9/10 family.</text>
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        <property type="protein sequence ID" value="BFG04057.1"/>
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        <property type="entry name" value="SPRY_RanBP9/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
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        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CTLH"/>
        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
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    <feature type="domain" description="CTLH" evidence="4">
        <location>
            <begin position="604"/>
            <end position="661"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="272"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="670"/>
            <end position="720"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="12"/>
            <end position="79"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="95"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="110"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
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            <begin position="180"/>
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            <begin position="204"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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            <begin position="230"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006535"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BFG04057.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="927" mass="100981" checksum="3940EF25738B57BF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G8W0</accession>
    <name>A0AAU9G8W0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Paired amphipathic helix protein Sin3a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Histone deacetylase complex subunit Sin3a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Transcriptional corepressor Sin3a</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_03204</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression. Required for cortical neuron differentiation and callosal axon elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with ARID4B, BRMS1L, HCFC1, HDAC1, HDAC2, MXI1, SAP30L, SAP130, SFPQ and TOPORS. Interacts with OGT (via TPRs 1-6); the interaction mediates transcriptional repression in parallel with histone deacetylase. Interacts with BAZ2A, MXD1, MXD3, MXD4, MBD2, DACH1, NCOR1, NR4A2, REST, RLIM, SAP30, SETDB1, SMYD2, and SUDS3. Interacts with PHF12 in a complex composed of HDAC1, PHF12 and SAP30. Interacts with TET1; the interaction recruits SIN3A to gene promoters. The large PER complex involved in the histone deacetylation is composed of at least HDAC1, PER2, SFPQ and SIN3A. Interacts with KLF11. Interacts with PPHLN1. Found in a complex with YY1, GON4L and HDAC1. Interacts (via PAH2) with FOXK1. Interacts with FOXK2. Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1. Interacts with SINHCAF. Interacts with SPHK2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome E"/>
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        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Paired amphipathic helix protein Sin3"/>
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    <dbReference type="FunFam" id="1.20.1160.11:FF:000002">
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        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013194">
        <property type="entry name" value="HDAC_interact_dom"/>
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    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF0">
        <property type="entry name" value="SIN3A, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
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    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00761">
        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="20">
        <location>
            <begin position="1124"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="218"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="711"/>
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    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="883"/>
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    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1087"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1404"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1475"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1528"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1898"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="2119"/>
            <end position="2145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="235"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="266"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="721"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1088"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1099"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1418"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1432"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1445"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1530"/>
            <end position="1543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1544"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="1840"/>
            <end position="1854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1898"/>
            <end position="1908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1915"/>
            <end position="1941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="2126"/>
            <end position="2142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="BFG04176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2174" mass="229606" checksum="67EBB45206246295" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G900</accession>
    <name>A0AAU9G900_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DMAD_03513</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G900"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032134">
        <property type="entry name" value="DUF4816"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16086">
        <property type="entry name" value="DUF4816"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BFG04579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="92" mass="10916" checksum="6A5EF416F9961A7C" modified="2024-11-27" version="1">MLLLLCLAVSCCLGGKTTTIVRHTDTLEPQADGFWANKTTWNARWVKYWRTKKIYEPVWKKVWTPTVHNEWVPLPNAPTEWEWEQEKGHHAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G901</accession>
    <name>A0AAU9G901_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_03548</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel HCN family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000083">
        <property type="entry name" value="Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000049">
        <property type="entry name" value="Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013621">
        <property type="entry name" value="Ion_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08412">
        <property type="entry name" value="Ion_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0114">cAMP</keyword>
    <keyword evidence="6" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0915">Sodium</keyword>
    <keyword evidence="4" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="14" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="747"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="826"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="908"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="983"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="20">
        <location>
            <begin position="1085"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="187"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="315"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="388"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="446"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="505"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="61"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="86"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="399"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="473"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="512"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="BFG04634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1289" mass="144264" checksum="04478CEE9E4BF521" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G903</accession>
    <name>A0AAU9G903_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="15">ATP-PFK</shortName>
            <shortName evidence="15">Phosphofructokinase</shortName>
            <ecNumber evidence="15">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_03389</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 15">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15 16">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="15">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 15 16">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="15"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G903"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000001">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000003">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000043">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase, platelet type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF4">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 15 16" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 15 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="17">
        <location>
            <begin position="20"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="17">
        <location>
            <begin position="411"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="15">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="15">
        <location>
            <begin position="411"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="90"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="120"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="166"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="210"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="300"/>
            <end position="303"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="537"/>
            <end position="541"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="575"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="582"/>
            <end position="584"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="638"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="664"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="670"/>
            <end position="673"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="745"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFG04413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="788" mass="86434" checksum="4F5233C1D0714E9F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G950</accession>
    <name>A0AAU9G950_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ion transport peptide-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_03079</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the arthropod CHH/MIH/GIH/VIH hormone family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG03986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G950"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2010.10:FF:000001">
        <property type="entry name" value="Ion transport peptide isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2010.10">
        <property type="entry name" value="Crustacean CHH/MIH/GIH neurohormone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018251">
        <property type="entry name" value="Crust_neurhormone_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031098">
        <property type="entry name" value="Crust_neurohorm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035957">
        <property type="entry name" value="Crust_neurohorm_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001166">
        <property type="entry name" value="Hyperglycemic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35981">
        <property type="entry name" value="ION TRANSPORT PEPTIDE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35981:SF2">
        <property type="entry name" value="ION TRANSPORT PEPTIDE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01147">
        <property type="entry name" value="Crust_neurohorm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00550">
        <property type="entry name" value="HYPRGLYCEMIC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81778">
        <property type="entry name" value="Crustacean CHH/MIH/GIH neurohormone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01250">
        <property type="entry name" value="CHH_MIH_GIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043706528" evidence="8">
        <location>
            <begin position="24"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="41"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="60"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR631098-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BFG03986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="121" mass="14195" checksum="E8F48C7B396EA1E5" modified="2024-11-27" version="1">MMCSRNIKISVVLFLVLIPIFTALPHNHNLSKRSNFFDLECKGIFNKTMFFRLDRICEDCYQLFRETSIHRLCKKDCFDSKWFGECLKVLLIPEEEVSNLQHFLRVVNGSPISFNMTPGQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G951</accession>
    <name>A0AAU9G951_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Mannosylglucosyl-3-phosphoglycerate phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_03130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-nucleotidase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G951"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07406">
        <property type="entry name" value="MPP_CG11883_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="entry name" value="5'-Nucleotidase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008334">
        <property type="entry name" value="5'-Nucleotdase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041821">
        <property type="entry name" value="CG11883_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575:SF48">
        <property type="entry name" value="5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575">
        <property type="entry name" value="5'-NUCLEOTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02872">
        <property type="entry name" value="5_nucleotid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01607">
        <property type="entry name" value="APYRASEFAMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="5">
        <location>
            <begin position="197"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="5'-Nucleotidase C-terminal" evidence="6">
        <location>
            <begin position="475"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="147"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG04085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="790" mass="86424" checksum="15BA0DC7AF2E75C4" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G954</accession>
    <name>A0AAU9G954_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CDK5 and ABL1 enzyme substrate 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_03223</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G954"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20556">
        <property type="entry name" value="CYCLIN_CABLES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000020">
        <property type="entry name" value="CDK5 and ABL1 enzyme substrate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012388">
        <property type="entry name" value="CABLES1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22896">
        <property type="entry name" value="CDK5 AND ABL1 ENZYME SUBSTRATE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22896:SF0">
        <property type="entry name" value="CYCLIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025798">
        <property type="entry name" value="Cables"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="6">
        <location>
            <begin position="608"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="72"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="197"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="397"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="409"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFG04201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="705" mass="77332" checksum="31CF9355B166A02D" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G955</accession>
    <name>A0AAU9G955_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Mitochondrial thiamine pyrophosphate carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_03422</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G955"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G958</accession>
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            <authorList>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <property type="term" value="P:cell morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:regulation of developmental process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.10.400.10:FF:000002">
        <property type="entry name" value="guanine nucleotide-binding protein G(Q) subunit alpha"/>
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        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
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        <property type="entry name" value="GproteinA_insert"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q) SUBUNIT ALPHA"/>
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        <property type="entry name" value="GPROTEINA"/>
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        <property type="entry name" value="GPROTEINAQ"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
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        <property type="entry name" value="G_ALPHA"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 10 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
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        <location>
            <position position="47"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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        <location>
            <begin position="149"/>
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        <ligand>
            <name>GTP</name>
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            <begin position="174"/>
            <end position="180"/>
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        <ligand>
            <name>GTP</name>
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    <feature type="binding site" evidence="10">
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            <position position="180"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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    <feature type="binding site" evidence="9">
        <location>
            <begin position="199"/>
            <end position="203"/>
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        <ligand>
            <name>GTP</name>
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        <ligand>
            <name>GTP</name>
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        <location>
            <position position="325"/>
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        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00007976"/>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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            <dbReference type="PIRSR" id="PIRSR601019-2"/>
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            <dbReference type="RuleBase" id="RU369122"/>
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            <dbReference type="EMBL" id="BFG04167.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
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    </evidence>
    <sequence length="353" mass="41227" checksum="1B005D01CFC433F3" modified="2024-11-27" version="1">MECCLSEEAKEQKRINQEIEKQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGYIKLVFQNIFMAMQSMIKAMDMLKISYGVGEHSELADLVMSIDYETVTTFEDPFLNAIKTLWADAGIQECYDRRREYQLTDSAKYYLSDLERIELPDFLPTEQDILRARVPTTGILEYPFDLDGIVFRMVDVGGQRSERRKWIHCFENVTSIIFLVALSEYDQILFESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPKQDHAAAKQFVLKKYLAANPDPERQCYSHFTTATDTENIKLVFCAVKDTIMQNALKEFNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G959</accession>
    <name>A0AAU9G959_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Paired amphipathic helix protein Sin3a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Histone deacetylase complex subunit Sin3a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Transcriptional corepressor Sin3a</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_03204</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression. Required for cortical neuron differentiation and callosal axon elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with ARID4B, BRMS1L, HCFC1, HDAC1, HDAC2, MXI1, SAP30L, SAP130, SFPQ and TOPORS. Interacts with OGT (via TPRs 1-6); the interaction mediates transcriptional repression in parallel with histone deacetylase. Interacts with BAZ2A, MXD1, MXD3, MXD4, MBD2, DACH1, NCOR1, NR4A2, REST, RLIM, SAP30, SETDB1, SMYD2, and SUDS3. Interacts with PHF12 in a complex composed of HDAC1, PHF12 and SAP30. Interacts with TET1; the interaction recruits SIN3A to gene promoters. The large PER complex involved in the histone deacetylation is composed of at least HDAC1, PER2, SFPQ and SIN3A. Interacts with KLF11. Interacts with PPHLN1. Found in a complex with YY1, GON4L and HDAC1. Interacts (via PAH2) with FOXK1. Interacts with FOXK2. Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1. Interacts with SINHCAF. Interacts with SPHK2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000001">
        <property type="entry name" value="Paired amphipathic helix protein Sin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000002">
        <property type="entry name" value="Paired amphipathic helix protein SIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000004">
        <property type="entry name" value="Paired amphipathic helix protein Sin3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.11">
        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013194">
        <property type="entry name" value="HDAC_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF0">
        <property type="entry name" value="SIN3A, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00761">
        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="20">
        <location>
            <begin position="1124"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="218"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="711"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="883"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1087"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1431"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1477"/>
            <end position="1509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1530"/>
            <end position="1577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1825"/>
            <end position="1869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1900"/>
            <end position="1949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="2121"/>
            <end position="2151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="235"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="266"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="721"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1088"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1099"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
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            <end position="1545"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1546"/>
            <end position="1562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="1842"/>
            <end position="1856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1900"/>
            <end position="1910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1917"/>
            <end position="1943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="2128"/>
            <end position="2144"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <authorList>
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                <person name="Yamamoto D."/>
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    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01463">
        <property type="entry name" value="vWA_VGCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000062">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000057">
        <property type="entry name" value="Voltage-dependent calcium channel subunit alpha-2/delta-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051173">
        <property type="entry name" value="Ca_channel_alpha-2/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013680">
        <property type="entry name" value="VDCC_a2/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013608">
        <property type="entry name" value="VWA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166:SF63">
        <property type="entry name" value="STRAIGHTJACKET, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2/DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08473">
        <property type="entry name" value="VGCC_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08399">
        <property type="entry name" value="VWA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043392565" evidence="16">
        <location>
            <begin position="27"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="17">
        <location>
            <begin position="267"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFG04216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1245" mass="143610" checksum="FCF5A207F38C0329" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G972</accession>
    <name>A0AAU9G972_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Adenylate kinase isoenzyme 6 homolog</fullName>
            <shortName evidence="10">AK6</shortName>
            <ecNumber evidence="10">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Dual activity adenylate kinase/ATPase</fullName>
            <shortName evidence="10">AK/ATPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_03207</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has also ATPase activity. Involved in the late cytoplasmic maturation steps of the 40S ribosomal particles, specifically 18S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit. Its NMP activity may have a role in nuclear energy homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer and homodimer. Interacts with small ribosomal subunit protein uS11. Not a structural component of 43S pre-ribosomes, but transiently interacts with them by binding to uS11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="10"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G972"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000372">
        <property type="entry name" value="Adenylate kinase isoenzyme 6 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595:SF0">
        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 10" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="NMPbind" evidence="10">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="10">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BFG04182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="175" mass="20394" checksum="D47DD350A113221F" modified="2024-11-27" version="1">MSELDADAKPNILITGTPGAGKSYLCERLAEQLNFDWLDCSKIAKEHDYIEEHDEEYDCPILDEDKLMDHLEPLMQKGGNIVEYHGCDFFPERWFQAVFVVTCPNKTLYDRLKERNYNEKKLSSNIECEIFGSILEEARESYKADIVHELCGETAADAAKSLKTVKQWYRMWKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G975</accession>
    <name>A0AAU9G975_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor 38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_03215</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PRP38 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G975"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005037">
        <property type="entry name" value="PRP38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024767">
        <property type="entry name" value="PRP38_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23142">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03371">
        <property type="entry name" value="PRP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12871">
        <property type="entry name" value="PRP38_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor 38 C-terminal" evidence="9">
        <location>
            <begin position="36"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="30"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="102"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG04192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="208" mass="26062" checksum="6035012E670262E7" modified="2024-11-27" version="1">MDEYIDELLRNDRVCDIILPRIQKRTILEENNEIEPKVSVLDEDLDDELPSEDEKPDDRETNRTNENSASSVRRPRRARSKSKSRSRERERDRRPAQSNNARSRDYYDELEDFERQRNRTRNRDTQHEDRRQPAGRQDRDREPRQDRDREQRQDRDRKPQNRDRERQDRDRMRERDRYGERDRRERENGSRHDQRERDSRNERERRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G979</accession>
    <name>A0AAU9G979_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Probable nuclear hormone receptor HR3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Nuclear receptor subfamily 1 group F member 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_03091</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Putative receptor whose ligand is not yet known.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G979"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06968">
        <property type="entry name" value="NR_DBD_ROR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000037">
        <property type="entry name" value="Hormone receptor 3, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000003">
        <property type="entry name" value="Nuclear orphan receptor ROR-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044101">
        <property type="entry name" value="NR_DBD_ROR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001728">
        <property type="entry name" value="ThyrH_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45805">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR HR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45805:SF2">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR HR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00546">
        <property type="entry name" value="THYROIDHORMR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 14 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="16">
        <location>
            <begin position="40"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="17">
        <location>
            <begin position="227"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="137"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BFG04016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="477" mass="53954" checksum="C714BD7CF31BAED5" modified="2024-11-27" version="1">MWSSVTSKLEAHANNLGQSNVQSPAGQNNSSGSIKAQIEIIPCKVCGDKSSGVHYGVITCEGCKGFFRRSQSSVVNYQCPRNKQCVVDRVNRNRCQYCRLQKCLKLGMSRDAVKFGRMSKKQREKVEDEVRFHRAQMRAQSDAAPDSSVYDTQTPSSSDQLHHNSYNSGGYSNNEVGYGSPYGYSASVTPQQTMQYDISADYVDSTTYEPRSTIIDPEFISHADGDINDVLIKTLAEAHANTNTKLEAVHDMFRKQQDISRILYYKNLGQEELWLDCAEKLTQMIQNIIEFAKLIPGFMRLSQDDQILLLKTGSFELAIVRMSRLLDLSQNAVLYGDVMLPQEAFYTSDSEEMRLVSRIFQTAKSIAELKLTETELALYQSLVLLWPERNGVRGNTEIQRLFNLSMNAIRQELETNHAPLKGDVTVLDTLLNNIPNFRDISILHMESLSKFKQTHPTVVFPALYKELFSIDSQQDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9G981</accession>
    <name>A0AAU9G981_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Myocyte-specific enhancer factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DMAD_03459</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9G981"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000001">
        <property type="entry name" value="Myocyte-specific enhancer factor 2A homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022102">
        <property type="entry name" value="HJURP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF534">
        <property type="entry name" value="MYOCYTE-SPECIFIC ENHANCER FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12347">
        <property type="entry name" value="HJURP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
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        <location>
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            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="335"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="444"/>
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        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
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        <location>
            <begin position="201"/>
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            <begin position="226"/>
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            <begin position="365"/>
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        <location>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        </source>
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        </source>
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        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <sequence length="529" mass="56512" checksum="94595D7D12A6093F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAA3</accession>
    <name>A0AAU9GAA3_DROMD</name>
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        <submittedName>
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        </submittedName>
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    </gene>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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    </dbReference>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC NOA-type" evidence="10">
        <location>
            <begin position="408"/>
            <end position="438"/>
        </location>
    </feature>
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        </location>
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        </source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="440" mass="50127" checksum="40301B7E506CF77B" modified="2024-11-27" version="1">MSEEESFVILGSSPVPSLCTDGSLLLADAMDGSDRPVPVTQERDGADVSAAGSSSGTLSTSVDSDRHKSLAASFIMGEVKSDVLKNSVYSQFPSLCSMQASAEDVVKLQNMMEEYVSLKKTLDMVNRTMMDYHKLTQQWRQEAADREEQCKQQLKECQEQVEKLRAENKALMAELEAKLVQIELVQDIRQKEQDELRQSVSEKTSLINNMRVEIDKLQQIELNSFEFVPHDDKKEAKEEVSNPLNYVQREEHDRQIRELQRELSKMLAEKLEVTDMKKLYIQEIDCLKVNLTSAEELMHKMERDINQLQATDIQRQEEIEHLQTQNEIYRRDFEMERADREKNAGEKEQYLLDLRSLQRRNQELIEALAESHKSPKSGSPSPSTSGVSSLREDQRPVRVLDANGADARPADSALRCPICSKGFNSLSVLQSHVNDCLDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAE8</accession>
    <name>A0AAU9GAE8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial</fullName>
            <ecNumber evidence="18">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_03941</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="16">Flavinylated by SdhE, about 5% flavinylation occurs in the absence of SdhE.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 18">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Mitochondrion inner membrane</location>
            <topology evidence="1 18">Peripheral membrane protein</topology>
            <orientation evidence="1 18">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="18"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAE8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045273">
        <property type="term" value="C:respiratory chain complex II (succinate dehydrogenase)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000004">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000482">
        <property type="entry name" value="Succinate dehydrogenase complex, subunit A, flavoprotein (Fp)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:001062">
        <property type="entry name" value="Succinate dehydrogenase complex, subunit A, flavoprotein (Fp)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.100:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit (SdhA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.40">
        <property type="entry name" value="succinate dehydrogenase protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011281">
        <property type="entry name" value="Succ_DH_flav_su_fwd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01816">
        <property type="entry name" value="sdhA_forward"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 16 18" id="KW-0274">FAD</keyword>
    <keyword evidence="6 16 18" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 18" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 18" id="KW-0813">Transport</keyword>
    <keyword evidence="5 18" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="19">
        <location>
            <begin position="59"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="20">
        <location>
            <begin position="509"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="64"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="453"/>
            <end position="454"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Tele-8alpha-FAD histidine" evidence="17">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000171-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="BFG05129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="660" mass="72379" checksum="5CA58F991DEBEF9C" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAF0</accession>
    <name>A0AAU9GAF0_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="11">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_03419</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="11"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAF0"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05236">
        <property type="entry name" value="FAR-N_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09071">
        <property type="entry name" value="FAR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000143">
        <property type="entry name" value="Fatty acyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033640">
        <property type="entry name" value="FAR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF12">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03015">
        <property type="entry name" value="Sterile"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0521">NADP</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Thioester reductase (TE)" evidence="13">
        <location>
            <begin position="19"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acyl-CoA reductase C-terminal" evidence="12">
        <location>
            <begin position="362"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFG04449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="506" mass="58408" checksum="4F1179B4FF1211D6" modified="2024-11-27" version="1">MTVDLSPVQEYYKDKTIFITGASGFMGKVLLEKLLYSCHSLKEVIIICRPKRGKTPESRLEEMYKLPIFQRIRDERPHMLKKVTIYQGDVTFDLLGLSGDSLKHVTENTNIVFHMAATLKLEGNLRDAIDMNLVGTKRALAVAKQMKHLEAFIHLSTAFCNCDQEVMYEKVYEFPHQPEDLIRLAEWMDVKTLDTITPDLLKPHPNTYTYSKRLAEILVRDHYESMPVIIARPSIVTPAVAEPLPGWVDNMNGPTGVLIGAGKGVIRSMICNGDLRSEVIPVDIAINGLILLPYHNSFVEERPLQIPVYNLTVDDAKKRTWKWVMDVGRDLGKKYPFEVGLWYPDGNMTSSKLYHTFCTIMFMWLPAYIIDFLLLIFGQRRFMVRVQTKVSVGLEVLQFFTTRNWDFKSTHFQQIYQKLGETDRKIFKINSDDVDDYEYMKLSILGGRQYVMKEPLTSLPKSRIQLRFLYCLDRICKTLIMTGLLYWSSQKLGLIELVRNGLFVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAG4</accession>
    <name>A0AAU9GAG4_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="11">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DMAD_03419</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="11"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAG4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05236">
        <property type="entry name" value="FAR-N_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09071">
        <property type="entry name" value="FAR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000143">
        <property type="entry name" value="Fatty acyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033640">
        <property type="entry name" value="FAR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF12">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03015">
        <property type="entry name" value="Sterile"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0521">NADP</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Thioester reductase (TE)" evidence="13">
        <location>
            <begin position="19"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acyl-CoA reductase C-terminal" evidence="12">
        <location>
            <begin position="362"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BFG04451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="499" mass="57400" checksum="7797C06DF95B80C0" modified="2024-11-27" version="1">MTVDLSPVQEYYKDKTIFITGASGFMGKVLLEKLLYSCHSLKEVIIICRPKRGKTPESRLEEMYKLPIFQRIRDERPHMLKKVTIYQGDVTFDLLGLSGDSLKHVTENTNIVFHMAATLKLEGNLRDAIDMNLVGTKRALAVAKQMKHLEAFIHLSTAFCNCDQEVMYEKVYEFPHQPEDLIRLAEWMDVKTLDTITPDLLKPHPNTYTYSKRLAEILVRDHYESMPVIIARPSIVSPSAYEPVPGWVDNLNGPTGLIVGAGKGVIRSMLIDTRHLSEVIPVDYAINGLCVIPYQFTGLKERPAEVPVYNITCADHRKMQWGEVIDMSKDIGYRYPFEAGLWYPDGCITTNRLHHKINVILFHWLPAYFIDFMLLLLGQKRFMVRIQNRISVGLEVLQFFTMRAWFFKSDAYSSLWNIMNDVDKKNFNMDMDPVETVPMYIESCVVGGRQYLMKESPDSLPRARLQLKLMYILDRVCKTVIVGSLCYWTYGVVARLLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAG5</accession>
    <name>A0AAU9GAG5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein lingerer</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DMAD_03952</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Acts in the nervous system to mediate the control of copulatory organs during courtship.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14277">
        <property type="entry name" value="UBA_UBP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000059">
        <property type="entry name" value="Lingerer, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051833">
        <property type="entry name" value="TC-DDR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022166">
        <property type="entry name" value="UBAP2/Lig"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16308:SF13">
        <property type="entry name" value="PROTEIN LINGERER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16308">
        <property type="entry name" value="UBIQUITIN ASSOCIATED PROTEIN 2-LIKE/LINGERER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12478">
        <property type="entry name" value="UBAP2-Lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0085">Behavior</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="129"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="335"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="472"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="512"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="568"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="637"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="771"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="928"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1136"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1190"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1241"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="142"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="205"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="441"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="512"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="532"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="570"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="639"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="771"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="841"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="938"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1241"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1252"/>
            <end position="1273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00056974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00074733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BFG05144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1281" mass="129131" checksum="6568A85B0A77D8CF" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAH1</accession>
    <name>A0AAU9GAH1_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_04338</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG05648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAH1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
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                <person name="Yamamoto D."/>
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        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="5">
        <location>
            <begin position="79"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="342"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="395"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="486"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="710"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="352"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="539"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="566"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="613"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="648"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="730"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BFG04617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="783" mass="86358" checksum="413E817C172B9D95" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9GAH6</accession>
    <name>A0AAU9GAH6_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Decapping nuclease</fullName>
            <ecNumber evidence="2">3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DMAD_03802</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the DXO/Dom3Z family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="2"/>
    <dbReference type="EMBL" id="AP029267">
        <property type="protein sequence ID" value="BFG04953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9GAH6"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110155">
        <property type="term" value="P:NAD-cap decapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013961">
        <property type="entry name" value="RAI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039039">
        <property type="entry name" value="RAI1-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395:SF9">
        <property type="entry name" value="DECAPPING AND EXORIBONUCLEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395">
        <property type="entry name" value="DOM-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08652">
        <property type="entry name" value="RAI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RAI1-like" evidence="3">
        <location>
            <begin position="28"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BFG04953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="378" mass="43938" checksum="4CA90EC4C1537C71" modified="2024-11-27" version="1">MNHEEIILNLKPHVITQEDRTVFPTFSKPQTHGGFSVSPDGKFEKNENRKRYLFVPPMGQIQVHLGDSEARRSQRLHLLQPSLDNILLYMEGKGTAFLQTKDQISVEVNHDIVCSSEVLELILRTPYEKEESWTLAITKYRNTIYISPVVLSEPNNSPMREMNAEWIETLRRHFLSEKPNTLPEPRGSMEQTGQYHGVFSVTINGKRFIFDAPVLAESVPNNANVKPETKEIFTDLQIRPDNMSVDEWTAHNRSQALKWWIKCFIVGIENIHVAYVNRHIIAHTIEKISVRKIFRDCETVWLPHVCFNFMARLVDNICSFTSSVDCHKTVYLLTFDAIPGKISYKCYPGRSQFTFIPDWFRIMLEERKEDLIREANVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9ETP1</accession>
    <name>A0AAU9ETP1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Phosphatidylinositol-binding clathrin assembly protein LAP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_08306</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Binds clathrin and phosphatidylinositol 4,5-bisphosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PICALM/SNAP91 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9ETP1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098894">
        <property type="term" value="C:extrinsic component of presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16985">
        <property type="entry name" value="ANTH_N_AP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000017">
        <property type="entry name" value="Phosphatidylinositol-binding clathrin assembly protein LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000001">
        <property type="entry name" value="phosphatidylinositol-binding clathrin assembly protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0168">Coated pit</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="12">
        <location>
            <begin position="16"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="597"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="677"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="306"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="699"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
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        </location>
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        </source>
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        </source>
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        <source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF89003.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1074" mass="120715" checksum="1A59AFBDCD54FEF5" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F5T8</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">DMAD_08913</name>
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    <organism evidence="2 3">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029263">
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
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        <location>
            <begin position="21"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BFF90376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="263" mass="29753" checksum="FF48F7E106C8FD9F" modified="2024-11-27" version="1">MSTSWPKLLLTALLLGQCQSQWKNDVPHVFAPLLPPTTSSHDAGKVNNVTLTPPGLIKQGRELELQLGLPPHAGSQRKLSRGKRFVAFPVGSSASAAVCLTTGVIGNPNLLYLSLGINWGVAYDLPNVTWVLQNAHGWTTKKSAQAQIKRRHRRELYSRLETMIDSMGYNGRDCVLRTLCESRQYFQRTKMNMVGEMLRTIFSLPKQRIFTRELHEDSDIVHYDKAYREAHKDDCAAQYDCHFSLLELAFGKYTTPPKNYYAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11 12">RF1</strain>
        </source>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
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    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
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            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
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            <end position="449"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022792"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF89138.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="459" mass="53032" checksum="5C8F97932FB2C67E" modified="2024-11-27" version="1">MANLVARLTVKTGALIIQRTSVGVAVCRYAKYSSGPANPGGFSKKTRRLFSLLGGSAFSVAAVIAIIKLRALDNPVNAVSLKKRMRDDSELENVKLTARERRFIKFASVEFDDQLYMTPQDFLESVVEQEPRPRLKRRQLSSDEVDKYKENTPALKKGSTRLFRNLRDKGIVSYTEYLFLLSILTKPKSGFRIAFNMFDTDGNQRVDKDEFLVIVSILAGAFKENQNVDPQTKRIMERIFSGAWKEKHGEQESQELEHEVPPIEKDYVNDGEGLQRRHVVPTTLQLHFFGKRGTGVINYDNFYRFMDNLQTEVLELEFHEFSKGNSIISELDFAKILLRYTYLATDEYDVFLERLLERVKDEKGITFHDFRDFCHFLNNLDDFTIAMRMYTLADRAISKEEFSRAVKICTGYSLSQHLIDTVFAIFDADGDGLLSYKEFIAIMKDRLHRGFKRHILFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F5U4</accession>
    <name>A0AAU9F5U4_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Glutamate-gated chloride channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_07964</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glutamate-gated chloride channel (TC 1.A.9.4) subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="BFF89153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F5U4"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
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        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008068">
        <property type="term" value="F:extracellularly glutamate-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18993">
        <property type="entry name" value="LGIC_ECD_GluCl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19062">
        <property type="entry name" value="LGIC_TM_GluCl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000024">
        <property type="entry name" value="Glutamate-gated chloride channel alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000022">
        <property type="entry name" value="glutamate-gated chloride channel isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044721">
        <property type="entry name" value="GluCl_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="309"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="34"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="251"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="BFF89153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="447" mass="51437" checksum="47779CA0CBBC1CDB" modified="2024-11-27" version="1">MGSGHYFWAIFYFASLCSASLANNAKINFREKEKKVLDQILGAGKYDARIRPSGINGTDGPAIVRINLFVRSIMTISDIKMEYSVQLTFREQWTDERLKFDDIQGRLKYLTLTEANRVWMPDLFFSNEKEGHFHNIIMPNVYIRIFPNGSVLYSIRISLTLACPMNLKLYPLDRQICSLRMASYGWTTNDLVFLWKEGDPVQVVKNLHLPRFTLEKFLTDYCNSKTNTGEYSCLKVDLLFKREFSYYLIQIYIPCCMLVIVSWVSFWLDQGAVPARVSLGVTTLLTMATQTSGINASLPPVSYTKAIDVWTGVCLTFVFGALLEFALVNYASRSDMHKENMKKKRRDLEQASLDAASDLLDTDSNATFAMKPLVRHPGDPLALEKRLQCEVHMQAPKRPNCCKTWLSKFPTRSKRIDVISRITFPLVFALFNLVYWSTYLFREEEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F5U8</accession>
    <name>A0AAU9F5U8_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Protein crumbs</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_08937</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the Crumbs protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048667">
        <property type="term" value="P:cell morphogenesis involved in neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009792">
        <property type="term" value="P:embryo development ending in birth or egg hatching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002064">
        <property type="term" value="P:epithelial cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060562">
        <property type="term" value="P:epithelial tube morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048592">
        <property type="term" value="P:eye morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048871">
        <property type="term" value="P:multicellular organismal-level homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051093">
        <property type="term" value="P:negative regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003002">
        <property type="term" value="P:regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048638">
        <property type="term" value="P:regulation of developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008593">
        <property type="term" value="P:regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061326">
        <property type="term" value="P:renal tubule development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003008">
        <property type="term" value="P:system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000185">
        <property type="entry name" value="basement membrane-specific heparan sulfate proteoglycan core protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000208">
        <property type="entry name" value="Crumbs 2, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000039">
        <property type="entry name" value="Crumbs cell polarity complex component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000123">
        <property type="entry name" value="Crumbs homolog 1 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000575">
        <property type="entry name" value="Crumbs, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000496">
        <property type="entry name" value="Crumbs, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000716">
        <property type="entry name" value="Crumbs, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000143">
        <property type="entry name" value="Crumbs, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000181">
        <property type="entry name" value="Crumbs, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000192">
        <property type="entry name" value="Crumbs, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000172">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000004">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000279">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000173">
        <property type="entry name" value="Neurogenic locus notch protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000565">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000122">
        <property type="entry name" value="Protein crumbs homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000511">
        <property type="entry name" value="protein crumbs isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000472">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916:SF13">
        <property type="entry name" value="SUSHI, VON WILLEBRAND FACTOR TYPE A, EGF AND PENTRAXIN DOMAIN-CONTAINING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00054">
        <property type="entry name" value="Laminin_G_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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</entry>
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF90721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="747" mass="82301" checksum="C9362320D017C1EB" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F659</accession>
    <name>A0AAU9F659_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Diacylglycerol kinase</fullName>
            <shortName evidence="15">DAG kinase</shortName>
            <ecNumber evidence="15">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DMAD_08245</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="15"/>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20800">
        <property type="entry name" value="C1_DGK_typeII_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20852">
        <property type="entry name" value="C1_DGK_typeII_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13274">
        <property type="entry name" value="PH_DGK_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09507">
        <property type="entry name" value="SAM_DGK-delta-eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000021">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000313">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000001">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000002">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000029">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000001">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054474">
        <property type="entry name" value="DGKD_4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF109">
        <property type="entry name" value="DIACYLGLYCEROL KINASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22944">
        <property type="entry name" value="DGKD_4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 15 22" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="18">
        <location>
            <begin position="82"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="19">
        <location>
            <begin position="195"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="19">
        <location>
            <begin position="267"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="DAGKc" evidence="21">
        <location>
            <begin position="349"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="20">
        <location>
            <begin position="1823"/>
            <end position="1886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="602"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="781"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="990"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1080"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="839"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="602"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="617"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="791"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1082"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1098"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1136"/>
            <end position="1146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="BFF89498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1886" mass="210200" checksum="536BC091DB215581" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F688</accession>
    <name>A0AAU9F688_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Phosphatidylinositol-binding clathrin assembly protein LAP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_08306</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Binds clathrin and phosphatidylinositol 4,5-bisphosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PICALM/SNAP91 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F688"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098894">
        <property type="term" value="C:extrinsic component of presynaptic endocytic zone membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16985">
        <property type="entry name" value="ANTH_N_AP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000017">
        <property type="entry name" value="Phosphatidylinositol-binding clathrin assembly protein LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000001">
        <property type="entry name" value="phosphatidylinositol-binding clathrin assembly protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0168">Coated pit</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="12">
        <location>
            <begin position="16"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="552"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="651"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="731"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="552"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="753"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF89573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="773" mass="82972" checksum="29F70134D5232EA4" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F691</accession>
    <name>A0AAU9F691_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Glutamate receptor ionotropic kainate 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DMAD_09266</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Postsynaptic cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF90811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAU9F691"/>
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        <property type="component" value="Chromosome O"/>
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        <property type="term" value="C:postsynaptic membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06382">
        <property type="entry name" value="PBP1_iGluR_Kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13714">
        <property type="entry name" value="PBP2_iGluR_Kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000178">
        <property type="entry name" value="Glutamate receptor subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000061">
        <property type="entry name" value="Glutamate receptor, ionotropic kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000010">
        <property type="entry name" value="Putative glutamate receptor ionotropic kainate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9 23" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043459832" evidence="20">
        <location>
            <begin position="19"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="548"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="623"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="813"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="21">
        <location>
            <begin position="417"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="22">
        <location>
            <begin position="428"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="871"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="871"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="893"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="503"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="508"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="675"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="676"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="724"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="16">
        <location>
            <position position="653"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="84"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="736"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BFF90811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="902" mass="100999" checksum="65324C7C131290B9" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9F694</accession>
    <name>A0AAU9F694_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Phosphatidylinositol-binding clathrin assembly protein LAP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_08306</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Binds clathrin and phosphatidylinositol 4,5-bisphosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PICALM/SNAP91 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029263">
        <property type="protein sequence ID" value="BFF89588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9F694"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome O"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098894">
        <property type="term" value="C:extrinsic component of presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16985">
        <property type="entry name" value="ANTH_N_AP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000017">
        <property type="entry name" value="Phosphatidylinositol-binding clathrin assembly protein LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000001">
        <property type="entry name" value="phosphatidylinositol-binding clathrin assembly protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0168">Coated pit</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="12">
        <location>
            <begin position="16"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="559"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="655"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="706"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="655"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="728"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF89588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="748" mass="80289" checksum="ED93C55D2F8831E8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FFS1</accession>
    <name>A0AAU9FFS1_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Intersectin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_12122</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FFS1"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097708">
        <property type="term" value="C:intracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150007">
        <property type="term" value="P:clathrin-dependent synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd11836">
        <property type="entry name" value="SH3_Intersectin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11838">
        <property type="entry name" value="SH3_Intersectin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11839">
        <property type="entry name" value="SH3_Intersectin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11840">
        <property type="entry name" value="SH3_Intersectin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000055">
        <property type="entry name" value="Intersectin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000046">
        <property type="entry name" value="intersectin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000192">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF170">
        <property type="entry name" value="DYNAMIN ASSOCIATED PROTEIN 160, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2029" mass="221915" checksum="CC6F58AE3993FF89" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FFX2</accession>
    <name>A0AAU9FFX2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ABC-type glutathione-S-conjugate transporter</fullName>
            <ecNumber evidence="13">7.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_12166</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>leukotriene C4(in) + ATP + H2O = leukotriene C4(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:38964"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.3" evidence="13"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015431">
        <property type="term" value="F:ABC-type glutathione S-conjugate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18595">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000812">
        <property type="entry name" value="multidrug resistance-associated protein 1 isoform X15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000074">
        <property type="entry name" value="Multidrug resistance-associated protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000041">
        <property type="entry name" value="Multidrug-Resistance like protein 1, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005292">
        <property type="entry name" value="MRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00957">
        <property type="entry name" value="MRP_assoc_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="477"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="561"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="594"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="992"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1036"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1109"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1134"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1219"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="18">
        <location>
            <begin position="334"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="651"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="18">
        <location>
            <begin position="996"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="1313"/>
            <end position="1547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="928"/>
            <end position="957"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFF94563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1552" mass="174026" checksum="3F96DA307127EC01" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FG30</accession>
    <name>A0AAU9FG30_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_12211</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FG30"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008510">
        <property type="term" value="F:sodium:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.930.10:FF:000008">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013769">
        <property type="entry name" value="Band3_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003024">
        <property type="entry name" value="Na/HCO3_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00834">
        <property type="entry name" value="ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF36">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07565">
        <property type="entry name" value="Band_3_cyto"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HCO3TRNSPORT"/>
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        <property type="entry name" value="NAHCO3TRSPRT"/>
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        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
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    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
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    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="509"/>
            <end position="531"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <end position="557"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="594"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="627"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="702"/>
            <end position="720"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="732"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="789"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="829"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="911"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="984"/>
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        <location>
            <begin position="137"/>
            <end position="433"/>
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    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="11">
        <location>
            <begin position="480"/>
            <end position="1019"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="436"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1037"/>
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        <location>
            <begin position="22"/>
            <end position="31"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="59"/>
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        <location>
            <begin position="74"/>
            <end position="96"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="101"/>
            <end position="111"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="294"/>
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            <begin position="447"/>
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        <location>
            <begin position="1045"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BFF94623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1184" mass="131018" checksum="6F415FA80B49233F" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FG34</accession>
    <name>A0AAU9FG34_DROMD</name>
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        <submittedName>
            <fullName evidence="7">Dedicator of cytokinesis protein 11</fullName>
        </submittedName>
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        <name evidence="7" type="ORF">DMAD_12234</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">RF1</strain>
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        <text evidence="2">Belongs to the DOCK family.</text>
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        <property type="protein sequence ID" value="BFF94650.1"/>
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        <property type="entry name" value="ZIZIMIN, ISOFORM K"/>
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        <property type="entry name" value="DHR-2_Lobe_A"/>
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        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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        <location>
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        <location>
            <begin position="787"/>
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        <location>
            <begin position="1802"/>
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        <location>
            <begin position="107"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1547"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2274"/>
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    </feature>
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        <location>
            <begin position="113"/>
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        </location>
    </feature>
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        <location>
            <begin position="125"/>
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    </feature>
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        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1563"/>
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        </location>
    </feature>
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        <location>
            <begin position="2275"/>
            <end position="2286"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF94650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2295" mass="260487" checksum="2E2EEE5D904F7F99" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FG70</accession>
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        <submittedName>
            <fullName evidence="12">Homeobox protein Mohawk</fullName>
        </submittedName>
    </protein>
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        <name evidence="12" type="ORF">DMAD_12254</name>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/IRO homeobox family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TALE/TGIF homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001654">
        <property type="term" value="P:eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TGFB-induced factor homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211:SF3">
        <property type="entry name" value="HOMEOBOX PROTEIN MOHAWK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 9 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="28"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="30"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="134"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="136"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BFF94693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="317" mass="36546" checksum="DB01D34307CE1A26" modified="2024-11-27" version="1">MEKTSSSRPKRNRRHSRRAWPPEDELQQPMRAAKRLFTPDIKRMLKDWLIRRRENPYPSREEKKQLAGETGLTYTQICNWFANWRRKLKNSEREKAKKSWGHLIKNYNHNARGNVEQFSISSEDSIWEEEMRPCVGEDDDGDYDDDELELSSRSTGSDGNANNASTAVYKPNFYVESGNMSEHSSQRIPMLPTVPVGRPKYKHQMMEKYLRDSTAEDAATGPTTTQPATATQLNKWLESAAKFTPDRNNYHIEWNTSRQKCGSGQGQGQGQERPVTATARGDGWMLHHKDELDAAEALANLAFNCRQRWHHMTTISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FG71</accession>
    <name>A0AAU9FG71_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DMAD_12252</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FG71"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="121"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BFF94690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="200" mass="23184" checksum="494DE5F33E5B371F" modified="2024-11-27" version="1">MSTTPHSLKTDVSMSHKSDLKSHLSKAALHQEFVPPQDLDISAIMYKYYLEEYRKYTPEERDSRLATFDAMDMLRTIEQIHNDVATMKLENYIMVEFLEKNDPKLLIGLRQRRTSILKRFQNKQRASFHGSQRMSSRQSSSKRSIPLSTSHLASLSPTATDRRRGTEYKLNFKAKAEMAEKRAAEVEKRVVDIERNGKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FG72</accession>
    <name>A0AAU9FG72_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DMAD_12224</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">RF1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FG72"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004742">
        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000010">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000003">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045257">
        <property type="entry name" value="E2/Pdx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151:SF90">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="6">
        <location>
            <begin position="83"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="179"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="240"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BFF94640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="494" mass="52966" checksum="F48E7115851FAA66" modified="2024-11-27" version="1">MLRSLATTRNELGALRAVLLRRPATACLRRGDGQVGVRALSTRLLTNARKLQASRSILVNSRPRSVNAWSYNFARNYASLPEHLRVPLPALSPTMERGSIVSWEKKEGDKLNEGDLLCEIETDKATMGFETPEEGYLAKILIPGGTKDVPIGQLLCIIVNDQASVAAFKDFKDDAPAAAPAAAAAPPPPPPPAAAAPAPVAAPPPAAAPAPAGTSQTAAEQRGDRVYASPMAKKLAEAQKLRLQGQQPAAAAKSAPPKAAPGARYTDIPVTTMRAVIAKRLLESKTQLPHYYVTAQCQVDNLLKFRAKVNKKYEKQGARVSVNDFIIKATAIASLKVPEANSAWMDSVIRQYDDVDVSVAVSTDKGLITPIIFGADRKGVLDISKDVKELAAKARANKLAPHEFQGGTISVSNLGMFGVNQFCAVINPPQSCILAIGTTTKQLVLDPDSLKGFKEINLLTVTLSADHRVVDGAVAARWLQHFRDYIEDPQNMIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FG80</accession>
    <name>A0AAU9FG80_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DMAD_12183</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">RF1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP029264">
        <property type="protein sequence ID" value="BFF94589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="4" xmlns="https://uniprot.org/uniprot">
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            <taxon>Insecta</taxon>
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                <person name="Yamamoto D."/>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="18 19">RF1</strain>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CD2_tandem_CHD5-9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEXHc_CHD6_7_8_9"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SF2_C_SNF"/>
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        </location>
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        </location>
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    </feature>
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        </source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
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    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="BFF94683.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="5513" mass="591428" checksum="B0126571EDF3C710" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FG93</accession>
    <name>A0AAU9FG93_DROMD</name>
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        <submittedName>
            <fullName evidence="11">Cilia- and flagella-associated protein 44</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DMAD_12285</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">RF1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
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    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0282">Flagellum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
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        <location>
            <begin position="465"/>
            <end position="498"/>
        </location>
    </feature>
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        <location>
            <begin position="1380"/>
            <end position="1416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1981"/>
            <end position="2005"/>
        </location>
    </feature>
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    </feature>
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        </location>
    </feature>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
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        </source>
    </evidence>
    <sequence length="2005" mass="233101" checksum="B6C4214F6DE4D7AD" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FG94</accession>
    <name>A0AAU9FG94_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Polycomb protein Sfmbt</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Scm-like with four MBT domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DMAD_12195</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Polycomb group (PcG) protein that binds to the Polycomb response elements (PREs) found in the regulatory regions of many genes. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Necessary but not sufficient to recruit a functional PcG repressive complex that represses target genes, suggesting that the recruitment of the distinct PRC1 complex is also required to allow a subsequent repression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with pho as a component of the pho-repressive complex (PhoRC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP029264">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome U"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20119">
        <property type="entry name" value="MBT_dSfmbt_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20122">
        <property type="entry name" value="MBT_dSfmbt_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20125">
        <property type="entry name" value="MBT_dSfmbt_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09580">
        <property type="entry name" value="SAM_Scm-like-4MBT"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
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            <begin position="742"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BFG00602.1"/>
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    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1270" mass="138829" checksum="C6DE1C45A024A766" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWS2</accession>
    <name>A0AAU9FWS2_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dynamin</fullName>
            <ecNumber evidence="2">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DMAD_00115</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome A"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
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        </source>
    </evidence>
    <sequence length="880" mass="98249" checksum="1BE8BE2DDB6E3577" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWW4</accession>
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        </submittedName>
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        <name evidence="2" type="ORF">DMAD_00030</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">RF1</strain>
        </source>
    </reference>
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        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
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        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BFF99899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="186" mass="21485" checksum="575AC1DE7CFBE137" modified="2024-11-27" version="1">MLRRLFEKLLDVAISTDLQLVDENTCRSAEKKPYDSLTIFTIVVLSVLCALMVLSTFYDYLFIEDQKQFSPLVKAFSARANSRVLFRIVDTKSNPNIIDCLHGMRCLSFIWVVYGHDYLVAAMGPNMNYVDMLTWFNSAFRMLITQGIYAVDTLFFLSGLLLVLIVLRVMERTKGKLNIPMMYLHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWW5</accession>
    <name>A0AAU9FWW5_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">trypsin</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DMAD_00260</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
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    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Blast:Serine protease nudel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043661599" description="trypsin" evidence="13">
        <location>
            <begin position="24"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="14">
        <location>
            <begin position="31"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BFG00223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="304" mass="33105" checksum="0BBD191B50ADB93C" modified="2024-11-27" version="1">MVHGLELGLGCFLWLLICCKVAAQTDTQPRIINGSVARVEETRHLVSIRLRKHDNNFGSGHICGGALIAANKVLTAAHCLYNNGRKRYRRPSELIVVMGTLNRYERPNGTIVSEVSSMAYMNTFSPDSMRDDVGVLFLRKALNSPNPFSVAPIQLARQVTPAGRVCQVAGWGRTEQSTLSNILMTANVSTIREQTCRSIYKSGLLPGMMCAGRLQGGTDSCQGDSGGPLVHEGRVIGVVSWGYGCAEPGLPGVYVDVQYYQQWIDERSGVARSRTSPGLGLSFSLLLGLLTFTATIQYSCRYNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWW6</accession>
    <name>A0AAU9FWW6_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Low-density lipoprotein receptor-related protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DMAD_00138</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">RF1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000214">
        <property type="entry name" value="low-density lipoprotein receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000212">
        <property type="entry name" value="Low-density lipoprotein receptor, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000171">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000008">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000065">
        <property type="entry name" value="Transmembrane protease serine 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000127">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000203">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000102">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000217">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050778">
        <property type="entry name" value="Cueball_EGF_LRP_Nidogen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513:SF44">
        <property type="entry name" value="LDL RECEPTOR RELATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513">
        <property type="entry name" value="VITELLOGENIN RECEPTOR-LIKE PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1900"/>
            <end position="1925"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="605"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="667"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="710"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="753"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="797"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="981"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="1024"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="1067"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="1111"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="1285"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="1328"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="1371"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="1415"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="1590"/>
            <end position="1633"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="1634"/>
            <end position="1676"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="1677"/>
            <end position="1720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="137"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1863"/>
            <end position="1882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="264"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="285"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="304"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="324"/>
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    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="343"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="393"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="415"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="434"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="458"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="465"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="477"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="522"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BFG00051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="2019" mass="227688" checksum="D399AD7C11E4F7D8" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWX2</accession>
    <name>A0AAU9FWX2_DROMD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Neurogenic protein mastermind</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DMAD_00597</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">RF1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:36684"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFG00662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FWX2"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349">
        <property type="entry name" value="ANKYRIN REPEAT AND LEM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349:SF2">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="92"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="313"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="677"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="751"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="861"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="899"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="403"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="692"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="751"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="782"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="861"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="899"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="918"/>
            <end position="937"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BFG00662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="952" mass="101592" checksum="94F0996D15812A18" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWX8</accession>
    <name>A0AAU9FWX8_DROMD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase</fullName>
            <ecNumber evidence="6">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DMAD_00041</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">RF1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 9">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="8">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="6"/>
    <dbReference type="EMBL" id="AP029266">
        <property type="protein sequence ID" value="BFF99914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAU9FWX8"/>
    <dbReference type="Proteomes" id="UP001500889">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042592">
        <property type="term" value="P:homeostatic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16212">
        <property type="entry name" value="EFh_NorpA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13361">
        <property type="entry name" value="PH_PLC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08591">
        <property type="entry name" value="PI-PLCc_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000024">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1230.10:FF:000005">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.240:FF:000001">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000008">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1230.10">
        <property type="entry name" value="Phospholipase C beta, distal C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016280">
        <property type="entry name" value="PLC-beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042531">
        <property type="entry name" value="PLC-beta_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037862">
        <property type="entry name" value="PLC-beta_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053945">
        <property type="entry name" value="PLCB1-4-like_EFh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF36">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE BETA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17787">
        <property type="entry name" value="PH_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22631">
        <property type="entry name" value="PLCB1-4-like_EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000956">
        <property type="entry name" value="PLC-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69989">
        <property type="entry name" value="C-terminal domain of PLC-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
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    <keyword evidence="4 6 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PI-PLC Y-box" evidence="12">
        <location>
            <begin position="550"/>
            <end position="666"/>
        </location>
    </feature>
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        <location>
            <begin position="666"/>
            <end position="794"/>
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        <location>
            <begin position="487"/>
            <end position="529"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="842"/>
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        <location>
            <begin position="999"/>
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        <location>
            <begin position="503"/>
            <end position="523"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="852"/>
            <end position="863"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1007"/>
            <end position="1031"/>
        </location>
    </feature>
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        <location>
            <position position="334"/>
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    <feature type="active site" evidence="7">
        <location>
            <position position="381"/>
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    <feature type="binding site" evidence="8">
        <location>
            <position position="335"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        <location>
            <position position="364"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
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    <feature type="binding site" evidence="8">
        <location>
            <position position="366"/>
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        <ligand>
            <name>Ca(2+)</name>
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        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000956-2"/>
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        <source>
            <dbReference type="RuleBase" id="RU361133"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50008"/>
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        <source>
            <dbReference type="EMBL" id="BFF99914.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001500889"/>
        </source>
    </evidence>
    <sequence length="1095" mass="124855" checksum="D0434D458AE4E2BC" modified="2024-11-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAU9FWY3</accession>
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        <submittedName>
            <fullName evidence="25">Basement membrane-specific heparan sulfate proteoglycan core protein</fullName>
        </submittedName>
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    <gene>
        <name evidence="25" type="ORF">DMAD_00392</name>
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        <name type="scientific">Drosophila madeirensis</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="30013"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25 26">RF1</strain>
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    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <location evidence="1">Extracellular matrix</location>
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        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="match status" value="3"/>
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        <property type="match status" value="13"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
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                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="9">Serine/threonine-protein phosphatase</fullName>
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        <name type="scientific">Drosophila madeirensis</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-02" db="EMBL/GenBank/DDBJ databases">
            <title>A chromosome-level genome assembly of Drosophila madeirensis, a fruit fly species endemic to Madeira island.</title>
            <authorList>
                <person name="Tomihara K."/>
                <person name="Llopart A."/>
                <person name="Yamamoto D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">RF1</strain>
        </source>
    </reference>
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        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
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            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
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            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
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            <name>Mn(2+)</name>
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        <text evidence="2">Belongs to the PPP phosphatase family. PP-1 subfamily.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
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        <property type="entry name" value="STPPase_N"/>
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    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF472">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE PP1-BETA CATALYTIC SUBUNIT"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
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        <property type="match status" value="1"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
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        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="11">
        <location>
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            <end position="125"/>
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